CN108967283B - Perinereis aibuhitensis freshness keeping method with addition of anti-stress factors - Google Patents
Perinereis aibuhitensis freshness keeping method with addition of anti-stress factors Download PDFInfo
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- CN108967283B CN108967283B CN201810636687.6A CN201810636687A CN108967283B CN 108967283 B CN108967283 B CN 108967283B CN 201810636687 A CN201810636687 A CN 201810636687A CN 108967283 B CN108967283 B CN 108967283B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/40—Culture of aquatic animals of annelids, e.g. lugworms or Eunice
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/03—Non-macromolecular organic compounds
- D21H17/05—Non-macromolecular organic compounds containing elements other than carbon and hydrogen only
- D21H17/06—Alcohols; Phenols; Ethers; Aldehydes; Ketones; Acetals; Ketals
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/03—Non-macromolecular organic compounds
- D21H17/05—Non-macromolecular organic compounds containing elements other than carbon and hydrogen only
- D21H17/14—Carboxylic acids; Derivatives thereof
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H21/00—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties
- D21H21/14—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties characterised by function or properties in or on the paper
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H27/00—Special paper not otherwise provided for, e.g. made by multi-step processes
Abstract
The invention provides a method for preserving perinereis aibuhitensis added with anti-stress factors, which comprises the steps of weighing and storing the living clamworm and bionic preservative paper according to the mass ratio of 3:1, firstly uniformly paving 1/3 bionic preservative paper at the bottom of a box, then paving the clamworm on the bottom preservative paper, and finally uniformly paving 2/3 bionic preservative paper on the surface of the clamworm; after the laying is finished, the surface of the box is tightly sleeved by a rubber band. The invention has the advantages of simple and easily obtained materials, simple and convenient operation method and low processing cost, the prepared preservative paper has light weight, softness, looseness, difficult hardening, good water absorption and moisture retention effects and high air permeability, can reduce the energy loss and abrasion of the clam worm activity, provides a suitable living environment for the clam worm, and improves the environmental adaptability and the anti-stress capability of the clam worm by adding sea mud, glucose, vitamin C and vitamin E anti-stress factors, particularly improves the anti-temperature difference stress capability, the anoxia tolerance and the immunity of the clam worm.
Description
Technical Field
The invention belongs to the field of clam worm storage, and particularly relates to a perinereis aibuhitensis freshness-retaining method added with an anti-stress factor.
Background
Nereid is a link animal growing on the coastal zone and the intertidal zone near the estuary, belonging to the phylum Annella, order Hymenopoda, family Nereidae and commonly known as "sea worm", "sea maggot", "sea centipede" and "sea leech". The Nereid contains abundant proteins, amino acids, unsaturated fatty acids, iron, phosphorus, vitamins and the like, has high nutritive value, is also rich in three enzyme systems of fibrinolytic enzyme, plasminogen activator, collagenase and the like, and is a multifunctional biological resource with rich nutrition, rapid growth and high yield value. In recent years, the method is widely applied to the fishing industry, water plant cultivation, medicine, pesticide, food and environmental monitoring.
With the development of the clam worm breeding industry, the increasing demand of people on clam worms is promoted by factors such as special aquaculture, fishing, medicinal use, eating and the like, so that the development of clam worm trade and export for outward transportation in various places is promoted, but the clam worms are extremely easy to die and are easily influenced by stress factors such as temperature change, infection, trauma, blood loss, oxygen deficiency, transportation and the like to cause the change of the function and metabolism of the clam worms, and the death of the clam worms can be caused by over-strong stress reaction or over-long duration. Naturally storing at normal temperature for 1-2 days at most. Therefore, it is very sensitive to the packing state and the storage and transportation time. In the prior art, the nereis bait for storage and transportation is usually prepared by sintering mica sheets at high temperature, namely vermiculite, and is used as a water-absorbing and heat-insulating liner in the process of storage and transportation of the nereis bait. However, the article is large and heavy, and the long-distance transportation cost is high; at the same time, although it can absorb water, it can not retain moisture, and can not absorb the peculiar smell discharged from Nereid, so that it is not favourable for Nereid to survive in long-distance transportation. At present, the problems of short time, incapability of keeping fresh for a long time and low survival rate of the clamworm during storage and transportation exist, and the clamworm is extremely easy to die in the storage and transportation process, so that the storage and transportation development of the clamworm is limited. Therefore, the invention provides a method for keeping the perinereis aibuhitensis fresh by adding an anti-stress factor aiming at the problems.
Disclosure of Invention
The invention aims to provide a perinereis aibuhitensis freshness-keeping method with an anti-stress factor added.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for keeping Nereid aibuhitensis fresh by adding anti-stress factors comprises weighing living Nereid and bionic preservative paper according to a mass ratio of 3:1, storing, uniformly paving 1/3 bionic preservative paper at the bottom of a box, paving the Nereid on the bottom preservative paper, and uniformly paving 2/3 bionic preservative paper on the surface of the Nereid; after the laying is finished, the surface of the box is tightly sleeved by a rubber band.
The bionic preservative paper comprises the following components in parts by weight: 10-20 parts of dry paper with the fiber content of more than 60 percent, 1-10 parts of sea mud with the salinity of 1.018-1.020 percent, 1-2 parts of glucose, 0.1-0.5 part of vitamin C and 0.1-1 part of vitamin E.
Preferably, the bionic preservative paper consists of the following components in parts by weight: 15 parts of dry paper with the fiber content of more than 60 percent, 5 parts of sea mud with the salinity of 1.018 percent, 1.25 parts of glucose, 0.25 part of vitamin C and 0.5 part of vitamin E.
The preparation method of the bionic preservative paper comprises the following steps:
1) soaking dry paper with the fiber content of more than 60% in seawater with the salinity of 1.018% -1.020% for more than 24 hours until the paper is flocculent;
2) beating the paper pulp obtained in the step 1) for 15-20min by using a beating machine to prepare soft, loose and non-coagulated paper pulp;
3) mixing the paper pulp obtained in the step 2) with sea mud, glucose, vitamin C and vitamin E, and stirring for 5-10min to fully fuse all the components;
4) dehydrating the paper pulp obtained in the step 3) to obtain paper pulp clusters with most of water removed;
5) dividing the paper mass obtained in the step 4) into small pieces, and stirring in a stirrer for 10-15min to a dispersed and uniform state;
6) drying the bionic preservative paper obtained in the step 5) in a drying oven at 60 ℃ until the moisture content is 15-20%, and during drying, turning over the preservative paper to ensure uniform drying;
7) crushing the preservative paper obtained in the step 6) in a crusher to obtain light, soft, loose and non-hardened bionic preservative paper;
8) storing the preservative paper obtained in the step 7) in a dry refrigerator with the temperature of 10-15 ℃.
The invention has the advantages that:
the material is simple and easy to obtain, the operation method is simple and convenient, the processing cost is low, the prepared preservative paper is light, soft and loose, is not easy to harden, has good water absorption and moisture retention effects and high air permeability, can reduce the energy loss and abrasion of the clam worm activity, provides a suitable living environment for the clam worm, and is added with sea mud, glucose, vitamin C and vitamin E anti-stress factors, so that the environmental adaptability and anti-stress capability of the clam worm are improved, and particularly the temperature difference stress resistance, the anoxia tolerance and the immunity of the clam worm are improved.
The perinereis aibuhitensis is fond to inhabit in the clam soil, the paper is used for replacing the beach soil environment in the proposal of the application, and seawater and sea mud are utilized to create a self-suitable ecological environment for the perinereis aibuensis. The sea mud is rich in mineral substances, algae elements, humus and the like, has a good moisturizing effect, and is capable of improving the adaptability of the clamworm to the environment, relieving the stress reaction, reducing the activity of the clamworm, and reducing the energy consumption and water loss. Glucose, vitamin C and vitamin E are used for improving the stress resistance of the clamworm, improving the temperature difference stress resistance, the anoxia tolerance and the immunity, and promoting the normal metabolism of the organism, thereby improving the survival rate or the growth rate of the clamworm.
Detailed Description
Example 1
A method for keeping the Nereid sericulture fresh with added anti-stress factors comprises the steps of weighing the living Nereid and the preservative paper according to a ratio of 3:1, storing the living Nereid and the preservative paper in a foam box with the specification of 10.5cm by 3.4cm of inner diameter, 12.5cm by 3.4cm of outer diameter and 1cm of wall thickness, uniformly paving 1/3 bionic preservative paper at the bottom of the box, paving the Nereid on the bottom preservative paper, and uniformly paving 2/3 bionic preservative paper on the surface of the Nereid sericulture; after the laying is finished, the surface of the box is tightly sleeved by a rubber band. Under the preservation condition of 10 ℃, the method is used for storing the clamworm, the survival time of the clamworm is as long as about 30 days, the preservation time is obviously prolonged compared with the natural preservation, and the survival rate reaches 100 percent.
The bionic preservative paper comprises the following components in parts by weight: 15 parts of dry paper with the fiber content of more than 60 percent, 5 parts of sea mud with the salinity of 1.018 percent, 1.25 parts of glucose powder, 0.25 part of vitamin C powder and 0.5 part of vitamin E powder.
The preparation method of the bionic preservative paper comprises the following steps:
1) soaking the dry paper in seawater with salinity of 1.018% for 36h until the paper is flocculent;
2) beating the paper pulp obtained in the step 1) for 18min by using a beating machine to prepare soft, loose and non-coagulated paper pulp;
3) mixing the paper pulp obtained in the step 2) with sea mud, glucose, vitamin C and vitamin E, and stirring for 8min to fully fuse all the components;
4) dehydrating the paper pulp obtained in the step 3) to obtain paper pulp clusters with most of water removed;
5) dividing the paper mass obtained in the step 4) into small pieces, and stirring in a stirrer for 12min to a dispersed and uniform state;
6) drying the bionic preservative paper obtained in the step 5) in a drying oven at 60 ℃ until the moisture content is 18%, and during drying, turning over the preservative paper should be paid attention to ensure uniform drying;
7) crushing the preservative paper obtained in the step 6) in a crusher to obtain light, soft, loose and non-hardened bionic preservative paper;
8) and (3) storing the preservative paper obtained in the step 7) in a dry refrigerator with the temperature of 12 ℃.
Example 2
A method for keeping Nereid Perinereis fresh with addition of anti-stress factor comprises weighing living Nereis Perrei and fresh-keeping paper at a ratio of 3:1, and storing in a foam box with inner diameter of 10.5cm 3.4cm, outer diameter of 12.5cm 3.4cm, and wall thickness of 1 cm. Firstly, uniformly paving 1/3 bionic preservative paper at the bottom of the box, then paving the clamworm on the bottom preservative paper, and finally uniformly paving 2/3 bionic preservative paper on the surface of the clamworm; after the laying is finished, the surface of the box is tightly sleeved by a rubber band. Under the preservation condition of 15 ℃, the method is used for storing the clamworm, the survival time of the clamworm is about 25 days, the preservation time is obviously prolonged compared with the natural preservation, and the survival rate reaches 99.9 percent.
The bionic preservative paper comprises the following components in parts by weight: 10 parts of dry paper with the fiber content of more than 60 percent, 2 parts of sea mud with the salinity of 1.020 percent, 1 part of glucose powder, 0.1 part of vitamin C powder and 0.1 part of vitamin E powder.
The preparation method of the bionic preservative paper comprises the following steps:
1) soaking the dry paper in seawater with salinity of 1.020% for 24h until the dry paper is flocculent;
2) beating the paper pulp obtained in the step 1) for 15min by using a beating machine to prepare soft, loose and non-coagulated paper pulp;
3) mixing the paper pulp obtained in the step 2) with sea mud, glucose, vitamin C and vitamin E, and stirring for 5min to fully fuse all the components;
4) dehydrating the paper pulp obtained in the step 3) to obtain paper pulp clusters with most of water removed;
5) dividing the paper mass obtained in the step 4) into small pieces, and stirring in a stirrer for 10min to a dispersed and uniform state;
6) drying the bionic preservative paper obtained in the step 5) in a drying oven at 60 ℃ until the moisture content is 15%, and during drying, turning over the preservative paper should be paid attention to ensure uniform drying;
7) crushing the preservative paper obtained in the step 6) in a crusher to obtain light, soft, loose and non-hardened bionic preservative paper;
8) and (3) storing the preservative paper obtained in the step 7) in a dry refrigerator with the temperature of 10 ℃.
Example 3
A method for keeping the Nereid sericulture fresh with added anti-stress factors comprises the steps of weighing the living Nereid and the preservative paper according to a ratio of 3:1, storing the living Nereid and the preservative paper in a foam box with the specification of 10.5cm by 3.4cm of inner diameter, 12.5cm by 3.4cm of outer diameter and 1cm of wall thickness, uniformly paving 1/3 bionic preservative paper at the bottom of the box, paving the Nereid on the bottom preservative paper, and uniformly paving 2/3 bionic preservative paper on the surface of the Nereid sericulture; after the laying is finished, the surface of the box is tightly sleeved by a rubber band. Under the preservation condition of 12 ℃, the method is used for storing the clamworm, the survival time of the clamworm is as long as about 30 days, the preservation time is obviously prolonged compared with the natural preservation, and the survival rate reaches 100 percent.
The bionic preservative paper comprises the following components in parts by weight: 20 parts of dry paper with the fiber content of more than 60 percent, 10 parts of sea mud with the salinity of 1.020 percent, 2 parts of glucose powder, 0.5 part of vitamin C powder and 1 part of vitamin E powder.
The preparation method of the bionic preservative paper comprises the following steps:
1) soaking the dry paper in seawater with salinity of 1.020% for 48h until the dry paper is flocculent;
2) beating the paper pulp obtained in the step 1) for 20min by using a beating machine to prepare soft, loose and non-coagulated paper pulp;
3) mixing the paper pulp obtained in the step 2) with sea mud, glucose, vitamin C and vitamin E, and stirring for 10min to fully fuse all the components;
4) dehydrating the paper pulp obtained in the step 3) to obtain paper pulp clusters with most of water removed;
5) dividing the paper mass obtained in the step 4) into small pieces, and stirring in a stirrer for 15min to a dispersed and uniform state;
6) drying the bionic preservative paper obtained in the step 5) in a drying oven at 60 ℃ until the moisture content is 20%, and during drying, turning over the preservative paper should be paid attention to ensure uniform drying;
7) crushing the preservative paper obtained in the step 6) in a crusher to obtain light, soft, loose and non-hardened bionic preservative paper;
8) and (3) storing the preservative paper obtained in the step 7) in a dry refrigerator with the temperature of 15 ℃.
The experimental study proves that the nereis stored by the method can survive for 25-30 days under the condition that the appropriate storage temperature of the nereis is 10-15 ℃, and the storage time is obviously prolonged compared with the natural storage. When the environmental temperature is changed, the Nereid can survive for 15-18 days when stored at the environmental temperature of 20 ℃, and can survive for 10-13 days when the environmental temperature reaches 25 ℃. When the environmental temperature reaches 30 ℃, the clamworm still can survive for about 5 days.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (3)
1. A preservation method of perinereis aibuhitensis added with anti-stress factors is characterized by comprising the following steps: weighing live clamworms and bionic preservative paper according to the mass ratio of 3:1 for storage, firstly uniformly paving 1/3 bionic preservative paper at the bottom of a box, then paving clamworms on the bottom preservative paper, and finally uniformly paving 2/3 bionic preservative paper on the surfaces of the clamworms; after the laying is finished, the surface of the box is tightly sleeved by a rubber band;
the bionic preservative paper comprises the following components in parts by weight: 10-20 parts of dry paper with the fiber content of more than 60 percent, 1-10 parts of sea mud with the salinity of 1.018-1.020 percent, 1-2 parts of glucose, 0.1-0.5 part of vitamin C and 0.1-1 part of vitamin E.
2. The preservation method of perinereis aibuhitensis added with the anti-stress factor according to claim 1, which is characterized in that: the bionic preservative paper comprises the following components in parts by weight: 15 parts of dry paper with the fiber content of more than 60 percent, 5 parts of sea mud with the salinity of 1.018 to 1.020 percent, 1.25 parts of glucose, 0.25 part of vitamin C and 0.5 part of vitamin E.
3. The preservation method of perinereis aibuhitensis added with the anti-stress factor according to claim 1, which is characterized in that: the preparation method of the bionic preservative paper comprises the following steps:
1) soaking dry paper with the fiber content of more than 60% in seawater with the salinity of 1.018% -1.020% for more than 24 hours until the paper is flocculent;
2) beating the paper pulp obtained in the step 1) for 15-20min by using a beating machine to prepare soft, loose and non-coagulated paper pulp;
3) mixing the paper pulp obtained in the step 2) with sea mud, glucose, vitamin C and vitamin E, and stirring for 5-10min to fully fuse all the components;
4) dehydrating the paper pulp obtained in the step 3) to obtain paper pulp clusters with most of water removed;
5) dividing the paper mass obtained in the step 4) into small pieces, and stirring in a stirrer for 10-15min to a dispersed and uniform state;
6) drying the bionic preservative paper obtained in the step 5) in a drying oven at 60 ℃ until the moisture content is 15-20%, and during drying, turning over the preservative paper to ensure uniform drying;
7) crushing the preservative paper obtained in the step 6) in a crusher to obtain light, soft, loose and non-hardened bionic preservative paper;
8) storing the preservative paper obtained in the step 7) in a dry refrigerator with the temperature of 10-15 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810636687.6A CN108967283B (en) | 2018-06-20 | 2018-06-20 | Perinereis aibuhitensis freshness keeping method with addition of anti-stress factors |
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| CN201810636687.6A CN108967283B (en) | 2018-06-20 | 2018-06-20 | Perinereis aibuhitensis freshness keeping method with addition of anti-stress factors |
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| Publication Number | Publication Date |
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| CN108967283A CN108967283A (en) | 2018-12-11 |
| CN108967283B true CN108967283B (en) | 2021-02-02 |
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Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3698358A (en) * | 1970-02-13 | 1972-10-17 | Takeda Chemical Industries Ltd | Method for maintaining freshness of aquatic animals |
| JPS56109555A (en) * | 1980-02-03 | 1981-08-31 | Nichiro Gyogyo Kk | Preservation of live bait for fishing |
| CN2383807Y (en) * | 1999-08-20 | 2000-06-21 | 陈宗远 | Box for transporting and storing living aquatics |
| CN103734093B (en) * | 2013-12-30 | 2016-04-27 | 盐城工学院 | Perinereis aibihitensis Grube heteronereis is cultivated and synchronous collection method |
| CN107149102A (en) * | 2017-05-22 | 2017-09-12 | 福建海唯美海洋生物科技有限公司 | The health-preserving food formula and manufacturing process of a kind of marine organisms |
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