CN108966748B - Starch anti-counterfeiting tobacco coated pelleted seed and preparation method and identification method thereof - Google Patents
Starch anti-counterfeiting tobacco coated pelleted seed and preparation method and identification method thereof Download PDFInfo
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Abstract
The invention discloses a starch anti-counterfeiting tobacco coating pelleted seed and a preparation method and an identification method thereof. According to the invention, the pure starch layer is added in the naked tobacco seed coating process, and the anti-counterfeiting detection is carried out by dripping iodine liquor on the section of the pelleted seed, so that the starch on the section of the anti-counterfeiting pelleted seed is annular in color development, attractive in appearance, good in anti-counterfeiting effect, high in identification speed, low in cost and high in accuracy.
Description
Technical Field
The invention belongs to the technical field of plant pelleted seed anti-counterfeiting, and particularly relates to a starch anti-counterfeiting tobacco coated pelleted seed as well as a preparation method and an identification method thereof.
Background
As an important agricultural production data, the quality of seeds affects not only the yield of crops, but also the quality of crops. The tobacco seeds are the source of tobacco production, are carriers for playing roles in tobacco science and technology and various tobacco production data, and play an irreplaceable role in the sustainable development of the tobacco industry.
In recent years, seed enterprises at home and abroad have conducted extensive research on seed anti-counterfeiting technology in order to ensure the authenticity of seeds and reduce the possibility of counterfeiting the seeds. At present, the anti-counterfeiting method of the seeds mainly comprises two anti-counterfeiting methods, namely seed product packaging anti-counterfeiting and seed self anti-counterfeiting. The anti-counterfeiting manufacture and detection of the seed product package are simple and convenient, but the defects are that the seed product package is easily copied by copying, and the authenticity of the seed product package cannot be guaranteed after the seed product package is separated from the package. In the aspect of self anti-counterfeiting of seeds, a fluorescent anti-counterfeiting technology, a magnetic powder anti-counterfeiting technology and the like appear in recent years, but the problems that the fluorescent anti-counterfeiting identification means is too single, the magnetic powder anti-counterfeiting process is more complex and the like exist.
In the prior art, the color development anti-counterfeiting pelleted tobacco seed and the processing and testing method thereof adopt the principle of color development of iodine when encountering starch for seed anti-counterfeiting, but still have a plurality of problems: firstly, the existing anti-counterfeiting detection method is to scatter anti-counterfeiting pelletized seeds containing starch into iodine tincture for cracking, observe the color development effect of the seed coating agent after cracking to judge the anti-counterfeiting effect of the seeds, and the volume ratio of the seeds used in the method is 1: 2, the iodine tincture is brown, and interferes with the judgment of the color development change of the cracked seed coating agent; secondly, in the detection method, the anti-counterfeiting pelleted seeds are cracked in iodine tincture and then develop color, the seed coating agent part of the cracked pelleted seeds is dissociated, the appearance of the pelleted seeds is deformed, the color development edge of the seed coating agent is fuzzy and not easy to observe, and the type judgment of the anti-counterfeiting seeds is influenced; thirdly, the detection method can reduce the detection effect recognizability of the sowed anti-counterfeiting pelleted seeds.
Therefore, in order to ensure the authenticity of the tobacco coated seeds, stop counterfeit phenomena, prevent overdue coated seeds from being used in a tobacco area, realize the rapid judgment of the authenticity of the tobacco coated seeds and effectively solve the existing defects, the invention needs to invent the starch anti-counterfeiting tobacco coated pelleted seeds, the preparation method and the identification method.
Disclosure of Invention
The invention aims to provide a starch anti-counterfeiting tobacco coated pelleted seed, a preparation method of the starch anti-counterfeiting tobacco coated pelleted seed and an identification method of the starch anti-counterfeiting tobacco coated pelleted seed.
The first purpose of the invention is realized by that, the starch anti-counterfeiting tobacco coating pelleted seed uses the conventional seed coating agent and the adhesive to pellet the tobacco naked seed coating to obtain the nucleating layer, then uses the conventional seed coating agent and the adhesive to carry out coating and pelleting to the seed to obtain the pelleted layer, the nucleating layer and/or the pelleted layer comprises the starch layer obtained by pelleting pure starch, and the tobacco coating pelleted seed with the starch layer in the nucleating layer and/or the pelleted layer is obtained.
The second purpose of the invention is realized by firstly using the conventional seed coating agent and the adhesive to carry out nucleation granulation on the seeds, adding 50-70% of corn starch of the weight of the naked seeds of the tobacco when the particle size of the seeds is 0.80-1.00 mm, and spraying the conventional adhesive to pelletize to form a nucleation layer starch layer; continuously pelleting the seeds to the particle size of 1.20 mm-1.40 mm by using a conventional seed coating agent and an adhesive, adding 60% -90% of corn starch in the weight of the naked tobacco seeds, and spraying the conventional adhesive to pelletize to form a pelleting layer starch layer; and (3) continuously using the conventional seed coating agent and the adhesive to pelletize the seeds to the target particle size, and obtaining the double-layer starch anti-counterfeiting tobacco coating pelletization seeds after screening, polishing, coloring and drying.
The second purpose of the invention can also be realized by firstly using the conventional seed coating agent and the adhesive to carry out nucleation granulation on the seeds, adding 50-70% of corn starch of the weight of the naked seeds of the tobacco when the particle size of the seeds is 0.80-1.00 mm, and spraying the conventional adhesive to pelletize to form a nucleation layer starch layer; and (3) continuously using the conventional seed coating agent and the adhesive to pelletize the seeds to the target particle size, and obtaining the starch anti-counterfeiting tobacco coating pelletized seeds of the nucleation layer after screening, polishing, coloring and drying.
The second purpose of the invention can also be realized by firstly using the conventional seed coating agent and the adhesive to carry out nucleation granulation and pelleting on the seeds, adding 60-90% of corn starch in the weight of the naked tobacco seeds when the particle size of the seeds is 1.20-1.40 mm, and spraying the conventional adhesive to pelletize to form a pelleted layer starch layer; and (4) continuously pelleting the seeds to a target particle size by using the conventional seed coating agent and the adhesive, and obtaining the pelleted seeds of the pelleted layer starch anti-counterfeiting tobacco coating after screening, polishing, coloring and drying.
The third purpose of the invention is realized by the method that 75% ethanol solution is used as solvent, and iodine solution with the mass fraction of 0.50% is prepared as color developing agent; and then cutting the starch anti-counterfeiting tobacco coated pelleted seeds along the central axis by using a medical dissecting blade, dropwise adding the color developing agent into the cut surface to perform iodine-starch color development reaction, observing the position where a blue color development ring appears, wherein the color development ring appears on the inner layer and the outer layer and is a double-layer starch anti-counterfeiting tobacco coated pelleted seed, the color development ring appears on the inner layer and is a nucleation layer starch anti-counterfeiting tobacco coated pelleted seed, and the color development ring appears on the outer layer and is a pelleted layer starch anti-counterfeiting tobacco coated pelleted seed.
Compared with the prior art, the invention has the following technical effects:
1. according to the invention, the pure starch layer is added in the naked tobacco seed coating process, and the anti-counterfeiting detection is carried out by dripping iodine liquor on the section of the pelleted seed, so that the starch on the section of the anti-counterfeiting pelleted seed is annular in color development, attractive in appearance, good in anti-counterfeiting effect, high in identification speed, low in cost and high in accuracy.
2. According to the processing process flow, when the naked tobacco seeds are nucleated and granulated to the particle size of 0.50 mm-0.60 mm, auxiliary materials such as bactericides, insecticides and the like are added. According to the invention, the starch layer is added when the seed nucleation granulation is carried out until the grain diameter is 0.80 mm-1.00 mm, so that the starch layer can be prevented from being mixed with the auxiliary materials, and the color development anti-counterfeiting effect can be ensured on the premise of not influencing the effects of the auxiliary materials.
3. The target grain diameter of the tobacco coated and pelleted seed is 1.60 mm-1.80 mm, and the seed surface needs to be colored according to the variety requirements (Honghuadajinyuan, MS Yunyan 87, MSK326 and the like) after being polished. According to the invention, the starch layer is added when the seeds are pelletized to the particle size of 1.20 mm-1.40 mm, so that the reduction of the anti-counterfeiting judgment reliability caused by the too close distance between the color developing ring and the appearance pigment can be avoided; meanwhile, the overlapping of two color development rings during the anti-counterfeiting detection of the pelleted tobacco seed containing the double starch layers can be avoided.
4. The invention is applied to the sown tobacco anti-counterfeiting pelleted seeds, and has obvious anti-counterfeiting detection effect and high identifiability.
5. Compared with a comparison document, the method can shorten the germination time of the anti-counterfeiting pelleted tobacco seeds under the indoor low-temperature germination condition and improve the germination index of the seeds.
6. The invention uses the original starch to carry out the coating and pelleting of the naked tobacco seeds, has simple operation and low cost and is convenient for expanding and popularizing.
Drawings
FIG. 1 is a cross-section color development effect diagram of safflower Honghuadajinyuan double-layer starch anti-counterfeiting coated pelleted seed before sowing;
FIG. 2 is a color development effect diagram of a sampling section of a safflower Honghua Dajinyuan double-layer starch anti-counterfeiting coated pelleted seed sowed for 5 d;
FIG. 3 is a cross-section color development effect diagram of MS Yunyan 87 nucleation layer starch anti-counterfeiting coated pelleted seed before sowing;
FIG. 4 is a color rendering effect diagram of a sampling section of MS cloud cigarette 87 nucleation layer starch anti-counterfeiting coated pelleted seed sowed for 5 d;
FIG. 5 is a cross-sectional color development effect diagram of the MSK326 pelleted layer starch anti-counterfeiting coated pelleted seed before sowing;
FIG. 6 is a color development effect diagram of a sampling section of a MSK326 pelleted layer starch anti-counterfeiting coated pelleted seed sowed for 5 d.
Detailed Description
The present invention is further illustrated by the following examples and the accompanying drawings, but the present invention is not limited thereto in any way, and any modifications or alterations based on the teaching of the present invention are within the scope of the present invention.
The starch anti-counterfeiting tobacco coating pelleted seed is prepared by pelleting a tobacco naked seed coating by using a conventional seed coating agent and an adhesive to obtain a nucleating layer, then continuously performing coating and pelleting by using the conventional seed coating agent and the adhesive to a target particle size to obtain a pelleted layer, wherein the nucleating layer and/or the pelleted layer comprise a starch layer obtained by pelleting pure starch to obtain the tobacco coating pelleted seed with the starch layer in the nucleating layer and/or the pelleted layer.
The starch content of the nucleation layer is 50-70% of the weight of the naked tobacco seeds, and the starch content of the pelleting layer is 60-90% of the weight of the naked tobacco seeds.
The starch is native starch, and the mesh range is 300-400 meshes.
The starch is corn starch.
The naked tobacco seeds are one or more of naked safflower Hongda, naked MS Yunyan 87 and naked MSK 326.
The target particle size is 1.60 mm-1.80 mm.
The preparation method of the starch anti-counterfeiting tobacco coated pelleted seed (the nucleating layer and the pelleted layer contain the starch layer) comprises the steps of firstly, carrying out nucleation granulation on the seed by using a conventional seed coating agent and an adhesive, adding 50-70% of corn starch of the weight of naked tobacco seeds when the particle size of the seed is 0.80-1.00 mm, and spraying the conventional adhesive to pelletize to form the starch layer of the nucleating layer; continuously pelleting the seeds to the particle size of 1.20 mm-1.40 mm by using a conventional seed coating agent and an adhesive, adding 60% -90% of corn starch in the weight of the naked tobacco seeds, and spraying the conventional adhesive to pelletize to form a pelleting layer starch layer; and (3) continuously using the conventional seed coating agent and the adhesive to pelletize the seeds to the target particle size, and obtaining the double-layer starch anti-counterfeiting tobacco coating pelletization seeds after screening, polishing, coloring and drying.
The preparation method of the starch anti-counterfeiting tobacco coating pelleted seed (the nucleation layer contains the starch layer) comprises the steps of firstly, carrying out nucleation pelleting on the seed by using a conventional seed coating agent and an adhesive, adding 50-70% of corn starch of the weight of naked tobacco seeds when the particle size of the seed is 0.80-1.00 mm, and spraying the conventional adhesive to pelletize to form the starch layer of the nucleation layer; and (3) continuously using the conventional seed coating agent and the adhesive to pelletize the seeds to the target particle size, and obtaining the starch anti-counterfeiting tobacco coating pelletized seeds of the nucleation layer after screening, polishing, coloring and drying.
The preparation method of the starch anti-counterfeiting tobacco coated and pelleted seed (the pelleted layer contains the starch layer) comprises the steps of firstly, carrying out nucleation granulation and pelleting on the seed by using a conventional seed coating agent and an adhesive, adding 60-90% of corn starch in the weight of naked tobacco seeds when the particle size of the seed is 1.20-1.40 mm, and spraying the conventional adhesive to pelletize to form the pelleted layer starch layer; and (4) continuously pelleting the seeds to a target particle size by using the conventional seed coating agent and the adhesive, and obtaining the pelleted seeds of the pelleted layer starch anti-counterfeiting tobacco coating after screening, polishing, coloring and drying.
The identification method comprises the steps of firstly using a 75% ethanol solution as a solvent, and preparing an iodine solution with the mass fraction of 0.50% as a color developing agent; and then cutting the starch anti-counterfeiting tobacco coated pelleted seeds along the central axis by using a medical dissecting blade, dropwise adding the color developing agent into the cut surface to perform iodine-starch color development reaction, observing the position where a blue color development ring appears, wherein the color development ring appears on the inner layer and the outer layer and is a double-layer starch anti-counterfeiting tobacco coated pelleted seed, the color development ring appears on the inner layer and is a nucleation layer starch anti-counterfeiting tobacco coated pelleted seed, and the color development ring appears on the outer layer and is a pelleted layer starch anti-counterfeiting tobacco coated pelleted seed.
Unless otherwise indicated, the proportions used in the present process are mass ratios.
Example 1
A double-layer starch anti-counterfeiting tobacco coating pelleted seed comprises two pelleting layers, a nucleating layer coated on the seed and a pelleting layer coated on the nucleating layer, wherein corn starch accounting for 60% of the weight of naked tobacco seeds is added in the nucleating layer, and corn starch accounting for 80% of the weight of the naked tobacco seeds is added in the pelleting layer.
The preparation method of the double-layer starch anti-counterfeiting tobacco coating pelleted seed comprises the following steps:
120 g and 160 g of corn starch with 300 meshes are weighed for later use. Adding 0.20 kg of safflower large golden element naked seeds into a coating machine for pelleting seeds, starting a power supply, spraying an adhesive to wet the surfaces of the seeds, spraying a seed coating agent to carry out nucleation and granulation, circularly spraying the adhesive and the seed coating agent until the particle size of the seeds is 0.90 mm, spraying the adhesive to wet the surfaces of the seeds, and adding 120 g of standby corn starch to carry out nucleation layer starch pelleting; after the starch layer pelleting of the nucleation layer is finished, continuously and circularly spraying the adhesive and the seed coating agent until the grain diameter of the seeds is 1.25 mm, and adding 160 g of standby corn starch for pelleting the starch layer pelleting of the pelleting layer; and after the pelleting of the starch layer of the pelleting layer is finished, continuously and circularly spraying the adhesive and the seed coating agent to finish the pelleting of the seed coating.
And screening out the coated and pelletized seeds with the diameter of 1.60 mm-1.80 mm, polishing and coloring by adopting a conventional method, and drying until the water content of the seeds is less than 2.0 percent, thus obtaining the safflower large jinyuan double-layer starch anti-counterfeiting coated and pelletized seeds.
Example 2
A nucleation layer starch anti-counterfeiting tobacco coating pelleted seed comprises two pelleting layers, a nucleation layer coated on the seed and a pelleting layer coated on the nucleation layer, wherein corn starch is added into the nucleation layer, and the weight of the corn starch is 60% of the weight of a naked tobacco seed.
The preparation method of the pelleted layer starch anti-counterfeiting tobacco coating pelleted seed comprises the following steps:
example 1 was repeated with the following differences: the adopted variety is MS Yunyan 87, 120 g of corn starch with 300 meshes is weighed for standby, and only 120 g of corn starch is added into a seed nucleating layer for pelleting.
Example 3
A pelleted starch anti-counterfeiting tobacco coated pelleted seed comprises two pelleted layers, a nucleating layer coated on the seed and a pelleted layer coated on the nucleating layer, wherein corn starch is added into the nucleating layer, and the weight of the corn starch is 80% of that of a naked tobacco seed.
The preparation method of the pelleted layer starch anti-counterfeiting tobacco coating pelleted seed comprises the following steps:
example 1 was repeated with the following differences: the adopted variety is MSK326, 160 g of corn starch with 300 meshes is weighed for standby, and only 160 g of corn starch is added into the seed pelleting layer for pelleting.
Example 4
The starch anti-counterfeiting tobacco coated and pelletized seeds prepared in the examples 1 to 3 and the corresponding varieties of coated and pelletized seeds without corn starch are used as materials for anti-counterfeiting identification. The specific method comprises the following steps: dissolving 2.00 g of elemental iodine into 456.02 ml of 75% ethanol to prepare an iodine color developing agent with the mass fraction of 0.50%, cutting 100 starch anti-counterfeiting tobacco coated and pelleted seeds prepared in the embodiments 1-3 and corresponding varieties of coated and pelleted seeds without corn starch along the central axis by using a medical dissecting blade, dripping 20 ul of the color developing agent into the cut surface to develop color, and observing the color development result.
The results show that: the three types of starch anti-counterfeiting tobacco coated and pelletized seeds can clearly display blue anti-counterfeiting rings, and the safflower large golden dollars, MS Yunyan 87 and MSK326 coated and pelletized seeds without corn starch have no color rings. The safflower gold double-layer starch anti-counterfeiting tobacco coating pelleted seed prepared in the example 1 has an inner blue anti-counterfeiting ring and an outer blue anti-counterfeiting ring which are clear, and a section color development effect chart before sowing is shown in a figure 1; the inner layer of the MS cloud cigarette 87 nucleating layer starch anti-counterfeiting tobacco coating pelleted seed prepared in the example 2 is provided with a clear blue anti-counterfeiting ring, and a section color development effect chart before sowing is shown in a figure 3; the outer layer of the MSK326 pelleted layer starch anti-counterfeiting tobacco pelleted seed prepared in the example 3 is provided with a clear blue anti-counterfeiting ring, and a section color development effect graph before sowing is shown in figure 5.
Example 5
Appearance quality parameters of the starch anti-counterfeiting tobacco coated and pelleted seeds prepared in examples 1-3 are measured according to GB/T25240-.
The results show that: the appearance quality of the double-layer starch anti-counterfeiting tobacco pelleted seed prepared in the example 1 meets the national standard requirements, the water content is 1.60%, the particle size is 1.68 mm, the uniformity is 95.70%, the single seed rate is 100.00%, the seed yield is 100.00%, the cracking rate is 100.00%, the cracking time is 63 s, and the single-seed compressive strength is 1.23N.
The appearance quality of the starch anti-counterfeiting tobacco pelleted seed with the nucleation layer prepared in the embodiment 2 meets the national standard requirements, the water content is 1.52%, the particle size is 1.75 mm, the uniformity is 95.50%, the single seed rate is 100.00%, the seed yield is 100.00%, the cracking rate is 100.00%, the cracking time is 16 s, and the single-seed compressive strength is 1.33N.
The appearance quality of the pelleted starch anti-counterfeiting tobacco coated pelleted seed prepared in the example 3 meets the national standard requirements, the water content is 1.85%, the particle size is 1.76 mm, the uniformity is 95.80%, the single seed rate is 100.00%, the cracking time is 32 s, and the single-seed compressive strength is 1.14N.
Example 6
Indoor germination detection is carried out on the starch anti-counterfeiting tobacco coated and pelleted seeds prepared in the embodiments 1-3 and the corresponding varieties of tobacco coated and pelleted seeds without corn starch at the temperature of 25 ℃. The specific method comprises the following steps: 2 layers of wet filter paper are padded in the culture dishes, 100 coated and pelleted seeds to be tested are evenly placed in each culture dish, and the culture dish is covered with a cover. Setting 4 times for each sample to be tested, germinating at 25 deg.C under light/dark culture for 12 h/12 h, keeping filter paper wet during germination, and counting the number of germinated seeds per day to 14 days. And (3) calculating the germination potential, the germination rate, the germination time and the germination index of the sample to be tested according to a formula: germination potential (%) = (number of seeds germinated in 7 days/number of seeds examined) × 100%; germination rate (%) = (number of seeds germinated in 14 days/number of seeds examined) × 100%; germination time (d) = Σ (Gt × Dt)/Σ Gt; germination index = Σ (Gt/Dt), where Gt is the number of sprouts at different times and Dt is the number of days of germination.
The test result shows that: the germination vigor, the germination rate, the germination time and the germination index of the safflower gold-coated and pelletized seeds prepared in example 1 are respectively 97.25%, 99.00%, 5.88 d and 17.03, and the germination rate, the germination time and the germination index of the safflower gold-coated and pelletized seeds without corn starch are respectively 97.25%, 99.00%, 5.79 d and 17.27. The analysis result of the difference significance shows that the germination potential, the germination rate, the germination time and the germination index of the safflower gold-element starch anti-counterfeiting tobacco coated and pelletized seed have no statistical difference with the corresponding parameters of the safflower gold-element tobacco coated and pelletized seed without adding the corn starch.
The germination vigor, the germination rate, the germination time and the germination index of the MS Yunyan 87 starch anti-counterfeiting tobacco coated and pelleted seeds prepared in the example 2 are respectively 96.50%, 97.50%, 5.75 d and 17.23, and the germination rate, the germination time and the germination index of the MS Yunyan 87 tobacco coated and pelleted seeds without corn starch are respectively 96.75%, 97.50%, 5.76 d and 18.09. The analysis result of the difference significance shows that the germination potential, the germination rate, the germination time and the germination index of the MS Yunyan 87 starch anti-counterfeiting tobacco pelleted seed have no statistical difference with the corresponding parameters of the MS Yunyan 87 tobacco pelleted seed without the corn starch.
The germination vigor, the germination rate, the germination time and the germination index of the MSK326 starch anti-counterfeiting tobacco coated and pelleted seeds prepared in example 3 are respectively 92.50%, 95.00%, 5.73 d and 16.93, and the germination rate, the germination time and the germination index of the MSK326 tobacco coated and pelleted seeds without corn starch are respectively 91.75%, 94.80%, 5.78 d and 16.79. The result of the analysis of the difference significance shows that the germination potential, the germination rate, the germination time and the germination index of the MSK326 starch anti-counterfeiting tobacco coated pelleted seed have no statistical difference with the corresponding parameters of the MSK326 tobacco coated pelleted seed without adding the corn starch.
Example 7
Indoor germination detection is carried out on the starch anti-counterfeiting tobacco coated and pelleted seeds prepared in the embodiments 1-3 and the corresponding varieties of tobacco coated and pelleted seeds without corn starch at the temperature of 15 ℃. The specific method comprises the following steps: 2 layers of wet filter paper are padded in the culture dishes, 100 coated and pelleted seeds to be tested are evenly placed in each culture dish, and the culture dish is covered with a cover. Setting 4 times for each sample to be tested, germinating at 25 deg.C under light/dark culture for 12 h/12 h, keeping filter paper wet during germination, and counting the number of seeds germinated every day to 21 days. Calculating the germination rate, the germination time and the germination index of the reference samples according to a formula: germination rate (%) = (number of seeds germinated in 14 days/number of seeds examined) × 100%; germination time (d) = Σ (Gt × Dt)/Σ Gt; germination index = Σ (Gt/Dt), where Gt is the number of sprouts at different times and Dt is the number of days of germination.
The test result shows that: the germination rate, the germination time and the germination index of the safflower gold-element starch anti-counterfeiting tobacco coated and pelletized seed prepared in the example 1 are respectively 97.25%, 11.40 d and 8.70, and the germination rate, the germination time and the germination index of the safflower gold-element tobacco coated and pelletized seed without the corn starch are respectively 98.00%, 11.71 d and 8.46. The difference significance analysis result shows that the germination rate of the safflower golden corn starch anti-counterfeiting tobacco coated and pelletized seed has no statistical difference compared with the germination rate of the safflower golden corn starch non-added tobacco coated and pelletized seed, but the germination time of the safflower golden corn starch anti-counterfeiting tobacco coated and pelletized seed is significantly shortened, and the germination index is significantly increased.
The germination rate, the germination time and the germination index of the MS Yunyan 87 starch anti-counterfeiting tobacco coating pelleted seed prepared in the example 2 are respectively 99.25%, 9.69 d and 10.30, and the germination rate, the germination time and the germination index of the MS Yunyan 87 tobacco coating pelleted seed without the corn starch are respectively 98.00%, 10.77 d and 9.26. The difference significance analysis result shows that the germination rate of the MS Yunyan 87 starch anti-counterfeiting tobacco coated pelleted seed has no statistical difference compared with the germination rate of the MS Yunyan 87 tobacco coated pelleted seed without corn starch, but the germination time of the MS Yunyan 87 starch anti-counterfeiting tobacco coated pelleted seed is obviously shortened, and the germination index is obviously increased.
The germination rate, the germination time and the germination index of the MSK326 starch anti-counterfeiting tobacco coated and pelleted seed prepared in example 3 are respectively 89.50%, 11.99 d and 7.58, and the germination rate, the germination time and the germination index of the MSK326 tobacco coated and pelleted seed without the corn starch are respectively 90.00%, 12.26 d and 7.27. The difference significance analysis result shows that the germination rate and the germination time of the MSK326 starch anti-counterfeiting tobacco coated pelleted seed have no statistical difference compared with the germination rate of the MSK326 coated pelleted seed without adding the corn starch, but the germination time of the MSK326 starch anti-counterfeiting tobacco coated pelleted seed is significantly shortened, and the germination index is significantly increased.
Example 8
The starch anti-counterfeiting tobacco coated and pelletized seeds prepared in the embodiments 1 to 3 and corresponding tobacco coated and pelletized seeds without corn starch are subjected to floating seedling emergence rate measurement and post-emergence anti-counterfeiting identification in a greenhouse. The specific method comprises the following steps: adopt 200 holes to educate seedling tray, fill moist seedling substrate, every hole seeding 1 seed that awaits measuring after the pressure hole, every sample that awaits measuring sows 3 seedling tray. After sowing for 5 days, dissolving 2.00 g of elemental iodine in 456.02 ml of 75% ethanol to prepare an iodine developing agent with the mass fraction of 0.50%; gently taking out the tobacco coated and pelleted seeds from the matrix by using tweezers, taking 50 granules for each treatment, and air-drying at room temperature; and (3) drying the taken tobacco coated pelleted seeds, cutting the pelleted seeds along the central axis by using a medical scalpel blade, dripping 20 ul of iodine color developing agent into the cut surfaces for developing, and observing the developing result. And (4) continuing to perform germination detection on the rest of the tobacco coated and pelleted seeds in the floating disc, and counting the rate of emergence in 14 days.
The test result shows that: after 5 d of sowing, performing anti-counterfeiting identification, wherein the double-layer color developing ring can be clearly observed on the anti-counterfeiting pelleted safflower golden starch seed prepared in the example 1, and the color developing effect graph of the sampling section after 5 d of sowing is shown in a figure 2; the MS Yunyan 87 starch anti-counterfeiting tobacco coating pelleted seed prepared in the embodiment 2 can clearly observe an inner layer color developing ring, and a color developing effect picture of a sampling section after 5 d sowing is shown in a figure 4; the MSK326 starch anti-counterfeiting tobacco coating pelleted seed prepared in the example 3 can clearly observe an outer layer color developing ring, and a color developing effect picture of a sampling section after 5 d sowing is shown in figure 6. The seedling emergence rate statistics is carried out after the seeds are sowed for 14 d, the seedling emergence rate of the anti-counterfeiting Honghuadajinyuan tobacco coated and pelleted seeds prepared in the example 1 is 95.82%, and compared with the seedling emergence rate of the Honghuadajinyuan tobacco coated and pelleted seeds without corn starch, the seedling emergence rate has no statistical difference; the emergence rate of the MS Yunyan 87 starch anti-counterfeiting tobacco pelleted seed prepared in the example 2 is 94.55%, and compared with the emergence rate of the MS Yunyan 87 tobacco pelleted seed without corn starch, the emergence rate of the seed has no statistical difference; the emergence rate of the MSK326 starch anti-counterfeiting tobacco coating pelleted seed prepared in the example 3 is 94.91%, and compared with the emergence rate of the MSK326 tobacco coating pelleted seed without adding the corn starch, the emergence rate of the MSK326 starch anti-counterfeiting tobacco coating pelleted seed is not statistically different.
Example 9
A starch anti-counterfeiting tobacco coating pelleted seed is characterized in that a conventional seed coating agent and an adhesive are used for pelleting a tobacco naked seed coating to obtain a nucleating layer, then the conventional seed coating agent and the adhesive are continuously used for pelleting to a target particle size to obtain a pelleted layer, 50% of corn starch of the weight of the tobacco naked seed is added into the nucleating layer, 60% of corn starch of the weight of the tobacco naked seed is added into the pelleted layer, and the seed is a double-layer starch anti-counterfeiting tobacco coating pelleted seed. The corn starch is native starch with 300 meshes.
The preparation method of the double-layer starch anti-counterfeiting tobacco coating pelleted seed comprises the following steps:
performing nucleation granulation on seeds by using a conventional seed coating agent and an adhesive, adding 50% of corn starch of the weight of naked tobacco seeds when the particle size of the seeds is 0.80mm, and spraying the conventional adhesive to perform nucleation layer starch layer pelletization; after the starch layer of the nucleation layer is pelletized, conventional seed coating agents and adhesives are used for pelletizing seeds until the particle size is 1.20mm, 60 percent of corn starch of the weight of naked tobacco seeds is continuously added, and the conventional adhesives are sprayed for pelletizing the starch layer of the pelletization layer; and after pelleting of the starch layer of the pelleting layer is finished, pelleting the seeds to a target particle size (1.60 mm-1.80 mm) by using a conventional seed coating agent and an adhesive, and screening, polishing, coloring and drying to obtain the double-layer starch anti-counterfeiting tobacco pelleted seeds.
The identification method described in this embodiment is to use 75% ethanol solution as solvent, and prepare iodine solution with mass fraction of 0.50% as color developing agent; and then cutting the starch anti-counterfeiting tobacco coated pelleted seed along the central axis by using a medical dissecting blade, dropwise adding the color developing agent into the section to perform iodine-starch color development reaction, observing the position where a blue color development ring appears, and allowing the inner layer and the outer layer to have color development rings to form double-layer starch anti-counterfeiting tobacco coated pelleted seeds.
Example 10
A starch anti-counterfeiting tobacco coating pelleted seed is characterized in that a conventional seed coating agent and an adhesive are used for pelleting a tobacco naked seed coating to obtain a nucleating layer, then the conventional seed coating agent and the adhesive are continuously used for pelleting to a target particle size to obtain a pelleted layer, corn starch is added into the nucleating layer, the amount of the corn starch is 70% of the weight of the tobacco naked seed, and the seed is the starch anti-counterfeiting pelleted seed with the nucleating layer. The corn starch is native starch with 325 meshes.
The preparation method of the nucleation layer starch anti-counterfeiting tobacco coating pelleted seed comprises the following steps:
performing nucleation granulation on seeds by using a conventional seed coating agent and an adhesive, adding corn starch accounting for 70% of the weight of naked tobacco seeds when the particle size of the seeds is 1.00mm, and spraying the conventional adhesive to perform nucleation layer starch layer pelletization; after the starch layer of the nucleation layer is pelletized, conventional seed coating agents and adhesives are used for pelletizing the seeds to a specified particle size (1.60 mm-1.80 mm), and the pelletized seeds of the starch anti-counterfeiting tobacco coating of the nucleation layer are obtained after screening, polishing, coloring and drying.
The identification method described in this embodiment is to use 75% ethanol solution as solvent, and prepare iodine solution with mass fraction of 0.50% as color developing agent; and then cutting the starch anti-counterfeiting tobacco coated pelleted seed along the central axis by using a medical dissecting blade, dropwise adding the color developing agent into the section to perform iodine-starch color development reaction, observing the position where a blue color development ring appears, and allowing a color development ring to appear on the inner layer to form the starch anti-counterfeiting tobacco coated pelleted seed with the nucleation layer.
Example 11
A starch anti-counterfeiting tobacco coating pelleted seed is characterized in that a conventional seed coating agent and an adhesive are used for pelleting a tobacco naked seed coating to obtain a nucleating layer, then the conventional seed coating agent and the adhesive are continuously used for pelleting to a target particle size to obtain a pelleted layer, and corn starch is added to the pelleted layer, wherein the weight of the corn starch is 90% of that of the tobacco naked seed, and the seed is the pelleted layer starch anti-counterfeiting tobacco coating pelleted seed. The corn starch is native starch with 400 meshes.
The preparation method of the pelleted layer starch anti-counterfeiting tobacco coating pelleted seed comprises the following steps:
performing nucleation granulation and pelleting on seeds by using a conventional seed coating agent and an adhesive, adding 90% of corn starch of the weight of naked tobacco seeds when the particle size of the seeds is 1.40mm, and spraying the conventional adhesive to perform pelleting layer starch layer pelleting; and after pelleting of the starch coating of the pelleting layer is finished, pelleting the seeds to a specified particle size (1.60 mm-1.80 mm) by using a conventional seed coating agent and an adhesive, and screening, polishing, coloring and drying to obtain the pelleted seeds of the starch anti-counterfeiting tobacco coating of the pelleting layer.
The identification method described in this embodiment is to use 75% ethanol solution as solvent, and prepare iodine solution with mass fraction of 0.50% as color developing agent; and then cutting the starch anti-counterfeiting tobacco coated pelleted seed along the central axis by using a medical dissecting blade, dropwise adding the color developing agent into the cut surface to perform iodine-starch color development reaction, observing the position where a blue color development ring appears, and allowing a color development ring to appear on the outer layer to obtain the pelleted starch anti-counterfeiting tobacco coated pelleted seed with the pelleted layer.
Example 12
A starch anti-counterfeiting tobacco coating pelleted seed is characterized in that a conventional seed coating agent and an adhesive are used for pelleting a tobacco naked seed coating to obtain a nucleating layer, then the conventional seed coating agent and the adhesive are continuously used for pelleting to a target particle size to obtain a pelleted layer, 55% of corn starch in weight of the tobacco naked seed is added into the nucleating layer, 70% of corn starch in weight of the tobacco naked seed is added into the pelleted layer, and the seed is a double-layer starch anti-counterfeiting tobacco coating pelleted seed. The corn starch is native starch with 300 meshes.
The preparation method of the double-layer starch anti-counterfeiting tobacco coating pelleted seed comprises the following steps:
performing nucleation granulation on seeds by using a conventional seed coating agent and an adhesive, adding 55 percent of corn starch of the weight of naked tobacco seeds when the particle size of the seeds is 0.85 mm, and spraying the conventional adhesive to perform nucleation layer starch layer pelletization; after the starch layer of the nucleation layer is pelletized, conventional seed coating agents and adhesives are used for pelletizing seeds until the particle size is 1.25 mm, corn starch with 70% of the weight of naked tobacco seeds is continuously added, and the conventional adhesives are sprayed for pelletizing the starch layer of the pelletization layer; and after pelleting of the starch layer of the pelleting layer is finished, pelleting the seeds to a target particle size (1.60 mm-1.80 mm) by using a conventional seed coating agent and an adhesive, and screening, polishing, coloring and drying to obtain the double-layer starch anti-counterfeiting tobacco pelleted seeds.
The identification method described in this embodiment is to use 75% ethanol solution as solvent, and prepare iodine solution with mass fraction of 0.50% as color developing agent; and then cutting the starch anti-counterfeiting tobacco coated pelleted seed along the central axis by using a medical dissecting blade, dropwise adding the color developing agent into the section to perform iodine-starch color development reaction, observing the position where a blue color development ring appears, and allowing the inner layer and the outer layer to have color development rings to form double-layer starch anti-counterfeiting tobacco coated pelleted seeds.
Example 13
A starch anti-counterfeiting tobacco coating pelleted seed is characterized in that a conventional seed coating agent and an adhesive are used for pelleting a tobacco naked seed coating to obtain a nucleating layer, then the conventional seed coating agent and the adhesive are continuously used for pelleting to a target particle size to obtain a pelleted layer, corn starch is added into the nucleating layer, the amount of the corn starch is 65% of the weight of the tobacco naked seed, and the seed is the starch anti-counterfeiting pelleted seed with the nucleating layer.
The preparation method of the nucleation layer starch anti-counterfeiting tobacco coating pelleted seed comprises the following steps:
performing nucleation granulation on seeds by using a conventional seed coating agent and an adhesive, adding 65 percent of corn starch of the weight of naked tobacco seeds when the particle size of the seeds is 0.95 mm, and spraying the conventional adhesive to perform nucleation layer starch layer pelletization; after the nucleation layer starch layer is pelletized, conventional seed coating agent and adhesive are used for pelletizing the seeds to a specified particle size, and the pelletized seeds are screened, polished, colored and dried to obtain the nucleation layer starch anti-counterfeiting tobacco coating seeds.
The identification method described in this embodiment is to use 75% ethanol solution as solvent, and prepare iodine solution with mass fraction of 0.50% as color developing agent; and then cutting the starch anti-counterfeiting tobacco coated pelleted seed along the central axis by using a medical dissecting blade, dropwise adding the color developing agent into the section to perform iodine-starch color development reaction, observing the position where a blue color development ring appears, and allowing a color development ring to appear on the inner layer to form the starch anti-counterfeiting tobacco coated pelleted seed with the nucleation layer.
Example 14
A starch anti-counterfeiting tobacco coating pelleted seed is characterized in that a conventional seed coating agent and an adhesive are used for pelleting a tobacco naked seed coating to obtain a nucleating layer, then the conventional seed coating agent and the adhesive are continuously used for pelleting to a target particle size to obtain a pelleted layer, and corn starch is added to the pelleted layer, wherein the weight of the corn starch is 85% of that of the tobacco naked seed, and the seed is the pelleted layer starch anti-counterfeiting tobacco coating pelleted seed.
The preparation method of the pelleted layer starch anti-counterfeiting tobacco coating pelleted seed comprises the following steps:
performing nucleation granulation and pelleting on seeds by using a conventional seed coating agent and an adhesive, adding corn starch accounting for 85% of the weight of naked tobacco seeds when the particle size of the seeds is 1.30 mm, and spraying the conventional adhesive to perform pelleting layer starch layer pelleting; after the pelleting layer starch layer is pelleted, the seeds are pelleted to the specified particle size by using the conventional seed coating agent and the adhesive, and the pelleted seeds of the pelleting layer starch anti-counterfeiting tobacco coating are obtained after screening, polishing, coloring and drying.
The identification method described in this embodiment is to use 75% ethanol solution as solvent, and prepare iodine solution with mass fraction of 0.50% as color developing agent; and then cutting the starch anti-counterfeiting tobacco coated pelleted seed along the central axis by using a medical dissecting blade, dropwise adding the color developing agent into the cut surface to perform iodine-starch color development reaction, observing the position where a blue color development ring appears, and allowing a color development ring to appear on the outer layer to obtain the pelleted starch anti-counterfeiting tobacco coated pelleted seed with the pelleted layer.
Claims (7)
1. A preparation method of starch anti-counterfeiting tobacco coating pelleted seeds is characterized in that conventional seed coating agents and adhesives are used for carrying out nucleation and pelleting on the seeds, when the particle size of the seeds is 0.80 mm-1.00 mm, 50% -70% of corn starch of the weight of naked tobacco seeds is added, and the conventional adhesives are sprayed for pelleting to form a nucleation layer starch layer; continuously pelleting the seeds to the particle size of 1.20 mm-1.40 mm by using a conventional seed coating agent and an adhesive, adding 60% -90% of corn starch in the weight of the naked tobacco seeds, and spraying the conventional adhesive to pelletize to form a pelleting layer starch layer; and (3) continuously using the conventional seed coating agent and the adhesive to pelletize the seeds to the target particle size, and obtaining the double-layer starch anti-counterfeiting tobacco coating pelletization seeds after screening, polishing, coloring and drying.
2. A preparation method of starch anti-counterfeiting tobacco coating pelleted seeds is characterized in that conventional seed coating agents and adhesives are used for carrying out nucleation and pelleting on the seeds, when the particle size of the seeds is 0.80 mm-1.00 mm, 50% -70% of corn starch of the weight of naked tobacco seeds is added, and the conventional adhesives are sprayed for pelleting to form a nucleation layer starch layer; and (3) continuously using the conventional seed coating agent and the adhesive to pelletize the seeds to the target particle size, and obtaining the starch anti-counterfeiting tobacco coating pelletized seeds of the nucleation layer after screening, polishing, coloring and drying.
3. A preparation method of starch anti-counterfeiting tobacco coating pelleted seeds is characterized in that conventional seed coating agents and adhesives are used for carrying out nucleation pelleting and pelleting on the seeds, when the particle size of the seeds is 1.20 mm-1.40 mm, 60% -90% of corn starch of the weight of naked tobacco seeds is added, and the conventional adhesives are sprayed for pelleting to form a pelleted layer starch layer; and (4) continuously pelleting the seeds to a target particle size by using the conventional seed coating agent and the adhesive, and obtaining the pelleted seeds of the pelleted layer starch anti-counterfeiting tobacco coating after screening, polishing, coloring and drying.
4. The method according to claim 1, 2 or 3, wherein the corn starch has a mesh size in the range of 300 to 400 mesh.
5. The method according to claim 1, 2 or 3, wherein the naked tobacco is one or more of naked Honghuadajinyuan, naked MS Yunyan 87, and naked MSK 326.
6. The method according to claim 1, 2 or 3, wherein the target particle size is 1.60mm to 1.80 mm.
7. The identification method of the starch anti-counterfeiting tobacco coated and pelleted seed obtained by the preparation method of claim 1, 2 or 3 is characterized in that 75% ethanol solution is used as a solvent, and iodine solution with the mass fraction of 0.50% is prepared as a color developing agent; and then cutting the starch anti-counterfeiting tobacco coated pelleted seeds along the central axis by using a medical dissecting blade, dropwise adding the color developing agent into the cut surface to perform iodine-starch color development reaction, observing the position where a blue color development ring appears, wherein the color development ring appears on the inner layer and the outer layer and is a double-layer starch anti-counterfeiting tobacco coated pelleted seed, the color development ring appears on the inner layer and is a nucleation layer starch anti-counterfeiting tobacco coated pelleted seed, and the color development ring appears on the outer layer and is a pelleted layer starch anti-counterfeiting tobacco coated pelleted seed.
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