CN105379470B - A kind of preparation method and its rapid identification method of anti-fake coat pelleted seeds - Google Patents
A kind of preparation method and its rapid identification method of anti-fake coat pelleted seeds Download PDFInfo
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- CN105379470B CN105379470B CN201510805038.0A CN201510805038A CN105379470B CN 105379470 B CN105379470 B CN 105379470B CN 201510805038 A CN201510805038 A CN 201510805038A CN 105379470 B CN105379470 B CN 105379470B
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- fake
- pulvis
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- tyrosine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/79—Photometric titration
Abstract
The method for anti-counterfeit of coat pelleted seeds, key step are as follows：（1）Tyrosine powder is sieved, the fine powder fallen from sieve pore is collected；（2）Fine powder is uniformly mixed with conventional tobacco seed pelleting pulvis, quality is the 2% of conventional coating pulvis, and new pulvis is as anti-fake coating pulvis after mixing；（3）Using above-mentioned anti-fake coating pulvis, seed is handled, obtains that there are anti-fraud functional coat pelleted seeds.The present invention does not change the technological process of tobacco seed coating Pelleting, and anti-fake core material tyrosine is cheap to be easy to get, and additive amount is small, keeps the method for anti-counterfeit application cost cheap；Tyrosine can be used as food additives as a kind of extremely common amino acid, act on human body, environment nonhazardous, also have no adverse effects to seed germination itself, growth, belong to a kind of harmless method for anti-counterfeit.The detection method of the method for anti-counterfeit is easy quickly, and accuracy is high, and it is anti-risk to be not easy the person of being counterfeited for strong security.
Description
Technical field
The invention belongs to the field of anti-counterfeit technology of vegetable seeds, be related to a kind of anti-fake coat pelleted seeds preparation method and
Its rapid identification method, the anti-fake processing especially suitable for tobacco seed.
Background technology
Seed decides agricultural output and quality, directly influences as the most direct carrier of agricultural science and technology, quality
The development of planting industry and farming industry.It is evaluation Seed enterprises competitiveness key factor for kind of a quality, but in market often
It will appear fake and forged seed, fake and forged seed quality is inferior, influences the yield and quality of crops, is not only caused to peasant household
Heavy losses have an effect on Seed enterprises prestige.Own kind, brand are protected in enhancing with Seed enterprises to peasant household's sense of responsibility
The enhancing of consciousness is protected, seed is anti-fake to be become Seed enterprises social responsibility, develop the important process of oneself.
Currently, the anti-counterfeiting technology that most of Seed enterprises use much all is realized by package means, such as entirely
Cease anti-fake, two-dimension code anti-counterfeit, printing is anti-fake, ink is anti-fake, extra package material is anti-fake etc..With the hair of packages printing technology
Exhibition, traditional package anti-counterfeiting technology are easily imitated by counterfeiter, and next year kind or false kind are put into personation is packed, is caused to peasant household
Heavy losses.In recent years, fluorescence falsification preventing technology and magnetic powder anti-counterfeiting technology are had also appeared, fluorescence falsification preventing identification of means is excessively single,
And magnetic powder false proof process is complex.
Tyrosine is the catalysis substrate of tyrosinase monophenolase function, is ultimately formed the main original of excellent melanocyte and pheomelanin
Material.In skin-lightening cosmetic research and development, can also have by studying the tyrosinase analogue that synthesis is competed with tyrosine
Effect ground inhibits the generation of melanocyte.Tyrosine is widely used in medicine and food additives, also artificial in beverage additive and preparation
It is applied in insect feedstuff.Not yet find that tyrosine is applied in plant breeding at present.
Currently, coat pelleted seeds technology is widely used in crop breeding, and especially in leaf tobacco production, tobacco
Seed all uses coat pelleted seeds substantially, and there are no find a kind of easy to operate, cost in the anti-counterfeiting technology of this kind of seed
It is cheap, it can fast and accurately detect the method for anti-counterfeit of the true and false of seed.
Invention content
For in the prior art, shortage is a kind of easy to operate, of low cost in coat pelleted seeds anti-counterfeiting technology and examines
Survey the true and false fast and accurately method for anti-counterfeit the problem of, the present invention provides the preparation method of a kind of anti-fake coat pelleted seeds and its fast
Fast identification method.This method particular content is as follows：
A kind of preparation method of anti-fake coat pelleted seeds, includes the following steps：
（1）By grain size it is 0.9mm mesh screens by tyrosine powder, is sieved, collect the tyrosine that is fallen from sieve pore
Fine powder；
（2）Tyrosine powder after screening is uniformly mixed with conventional tobacco seed pelleting pulvis, quality is conventional coating pulvis
2%, new pulvis is as anti-fake coating pulvis after mixing；
（3）Using above-mentioned anti-fake coating pulvis, vernalization initiation, granulation, Pelleting coating, polishing, dry etc. are carried out to seed
Processing, obtains having anti-fraud functional coat pelleted seeds.
The rapid identification method of above-mentioned anti-fake coat pelleted seeds, is carried out in two steps：
（1）It is 1 that the first step, which is with volume ratio,:After 1 formic acid, acetic acid mixture dissolve coating agent, by dissolved packet
Clothing solution, point drip on silica gel plate, silica gel plate are placed under 254nm ultraviolet lamps, are in apparent blue light at silica gel plate drop
Spot；
（2）Second step is that above-mentioned silica gel plate is immersed the ninhydrin ethyl alcohol that prepared mass percent is 0.25% in advance
In solution, after taking-up, apparent yellow spotting is presented at drop for dry heat.
It is exactly anti-fake coat pelleted seeds that blue hot spot and the seed of yellow spotting is presented respectively in the identification of above two step.
The present invention does not change the technological process of tobacco seed coating Pelleting, and anti-fake core material tyrosine is cheap easily
, and additive amount is small, keeps the method for anti-counterfeit application cost cheap；During authentication, it need to only be directed to coating pulvis and be grasped
Make, not damaged to seed itself, i.e., the method is Nondestructive Identification；Tyrosine can be used as food as a kind of extremely common amino acid
Product additive acts on human body, environment nonhazardous, also has no adverse effects to seed germination itself, growth, belongs to a kind of harmless
Method for anti-counterfeit.
The evaluation program step of this anti-fake coat pelleted seeds is less, and the material and tool used is less, in qualification process
Occur two states, have identification speed it is fast, it is at low cost, accuracy it is high the advantages of.
Specific implementation mode
The embodiment of the present invention is given below, these embodiments are only a kind of special cases in protection domain.
In following embodiments, the kind of plant chooses tobacco bred：MS clouds and mists 87 and the big gold dollar naked seed of safflower, conventional coating
Pulvis, adhesive, coating equipment in sugar production line etc. are all from China Tobacco Seed Yuxi Co., Ltd., and tyrosine is purchased from the auspicious immortal object section in Shanghai
Skill Co., Ltd.
Embodiment one
（One）The preparation of anti-counterfeiting tobacco pelleted seed
Take 50g tyrosine powder to be poured on the 0.9mm mesh screens of aperture, shake mesh screen, collect fallen from sieve pore it is uniform,
Careful tyrosine powder bulk or the thicker powder of grain size need to be ground to its can be by mesh screen until；
50g tyrosine powder after screening is uniformly mixed with 2.5kg conventional tobacco seed pelleting pulvis, coating pulvis is made；
According to YC/T368-2010《Tobacco seed primed and pelleted seeds production technology regulation》It is required that with above-mentioned packet
Clothing agent is granulated MS clouds and mists 87 and the big gold dollar naked seed of safflower, Pelleting coating, polishing, is dried, and anti-fake coating is produced
Pelleted seed.
（Two）The identification method of above-mentioned anti-counterfeiting tobacco coat pelleted seeds is as follows：
Take it is above-mentioned have anti-fraud functional 2 capsuled seeds, crumb coating take out naked seed, the coating auxiliary material crumbed is placed in
In 0.5mlEP pipes, it is 1 that 80ul volume ratios, which are added,:1 formic acid, acetic acid mixture, cover tightly EP pipe lids, firmly shake, and stand, wait for
Liquid, solids stratification dip upper solution with 0.3mm capillaries, and same position repeats point sample 8 times on silica gel plate, by silica gel
Plate, which is entirely placed under the ultraviolet light of 254nm, to be irradiated, and clearly blue light is shown immediately at anti-fake seed point sample on silica gel plate
Spot.
0.2g ninhydrins are taken to be dissolved in ethyl alcohol l00ml, it is that 0.25% ninhydrin solution is spare to prepare mass fraction.Use tweezers
The silica gel plate after anti-fake seed point sample is gripped, prepared ethanol solution of ninhydrin in advance is immersed, is ensured complete with solution at point sample
Full connected after 2 seconds, takes out silica gel plate, is heated 2 minutes at hair-dryer alignment point sample or silica gel plate is placed in 120 DEG C of baking sheets
Clearly yellow spotting is presented in heating 1 minute on silica gel plate at anti-fake seed point sample.
Contrast experiment
For the practical application effect of the prominent present invention, spy is following contrast test
Contrast experiment one
Embodiment one is repeated, there is following difference：125g tyrosine and 2.5kg conventional tobacco seed pelleting pulvis is uniform
Mixing, obtains anti-fake coating pulvis.
It is mixed respectively with MS clouds and mists 87 and the big gold dollar naked seed of safflower with anti-fake coating pulvis, anti-counterfeiting tobacco Pelleting coating is made
Seed.
Take conventional tobacco seed-coating pelleted seed as a control group, hereinafter referred to as CK.
（One）Quality versus's experiment is done to above two groups of seeds.
Experimental result such as table 1.
Table 1：Quality versus's experimental result of anti-fake coat pelleted seeds and control group
Experiment shows：By table 1 the results show that anti-fake capsuled seed presentation quality after doping tyrosine with to compare CK basic
Indistinction, water content less than 3.0%, single seed rate and there is seed rate to be more than 99.0%, cleavage rate 100%, grain size 1.6 ~ 1.8mm it
Between, for simple grain compression strength between 1.0 ~ 3.0N, the uniformity is more than 95.0%, meets standard GB/T/T25240-2010《Tobacco
Coat pelleted seeds》Requirement；And the germination percentage of anti-fake capsuled seed and CK seeds are also suitable, tyrosine mass fraction is 2% real
It is even better to apply example 1, shows anti-fake coating pulvis on seed vitality without influence.
（Two）The identification of anti-counterfeiting tobacco coat pelleted seeds
It takes respectively conventional and there are anti-fraud functional MS clouds and mists 87 and each 2 of the big gold dollar coat pelleted seeds of safflower, take
Go out naked seed, coating auxiliary material is respectively placed in after crumbing in 0.5mlEP pipes, and it is 1 that 80ul volume ratios, which are added,:1 formic acid, acetic acid mixing
Liquid covers tightly EP pipe lids, firmly shakes, and stands, waits for liquid, solids stratification, dip upper solution with 0.3mm capillaries, exist respectively
Same position repeats point sample 8 times on silica gel plate, is respectively entirely placed on silica gel plate under the ultraviolet light of 254nm and irradiates, conventional
Seed on silica gel plate at point sample without color change, and show clearly blue light immediately on the silica gel plate of anti-fake seed point sample
Spot.
0.2g ninhydrins are taken to be dissolved in ethyl alcohol l00ml, it is that 0.25% ninhydrin solution is spare to prepare mass fraction.Use tweezers
Silica gel plate after conventional seed and anti-fake seed point sample is gripped respectively, is immersed prepared ethanol solution of ninhydrin in advance, is ensured point
It is completely attached to solution at sample, after 2 seconds, takes out silica gel plate, heated 2 minutes at hair-dryer alignment point sample or be placed on silica gel plate
It is heated 1 minute in 120 DEG C of baking sheets, without color change at conventional seed point sample, and clearly yellow is presented at anti-fake seed point sample
Spot.
Test result is shown in Table 2.
Table 2：The antifalse effect contrast and experiment of anti-fake coat pelleted seeds and control group
Experiment shows：By anti-fake treated seed, significant reaction is detected, accuracy rate is higher, the processed seed in end,
It detects unchanged.
Claims (2)
1. a kind of preparation method of anti-fake coat pelleted seeds, it is characterised in that：It mainly includes the following steps that：
（1）By aperture it is 0.9mm mesh screens by tyrosine powder, is sieved, collect the tyrosine fine powder that is fallen from sieve pore；
（2）Tyrosine powder after screening is uniformly mixed with conventional tobacco seed pelleting pulvis, quality is the 2% of conventional coating pulvis,
New pulvis is as anti-fake coating pulvis after mixing；
（3）Using above-mentioned anti-fake coating pulvis, vernalization initiation is carried out to seed, granulation, Pelleting coating, polishing, is dried, is obtained
To anti-fake coat pelleted seeds.
2. a kind of rapid identification method of anti-fake coat pelleted seeds, it is characterised in that：It is divided to following two steps to carry out：
（1）It is 1 that the first step, which is with volume ratio,:1 formic acid, acetic acid mixture are chosen and contain junket by method of claim 1 is obtained
The anti-fake coat pelleted seeds of propylhomoserin powder, its coating agent is dissolved, and by dissolved Coating Solution, point drips on silica gel plate, will
Silica gel plate is placed under 254nm ultraviolet lamps, in apparent blue hot spot at silica gel plate drop；
（2）Second step is that above-mentioned silica gel plate is immersed the ethanol solution of ninhydrin that prepared mass percent is 0.25% in advance
In, after taking-up, apparent yellow spotting is presented at drop for dry heat.
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| CN201510805038.0A CN105379470B (en) | 2015-11-20 | 2015-11-20 | A kind of preparation method and its rapid identification method of anti-fake coat pelleted seeds |
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| CN105379470A CN105379470A (en) | 2016-03-09 |
| CN105379470B true CN105379470B (en) | 2018-09-25 |
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| CN108966748B (en) * | 2018-08-01 | 2021-06-01 | 玉溪中烟种子有限责任公司 | Starch anti-counterfeiting tobacco coated pelleted seed and preparation method and identification method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101322455A (en) * | 2008-07-28 | 2008-12-17 | 李海涛 | Compound aminoacid seed afterbirth substrate for treating sandy wasteland |
| CN101403694A (en) * | 2008-10-31 | 2009-04-08 | 浙江大学 | Fluorescence anti-counterfeit and detection method for pellet seed |
| CN101971727A (en) * | 2010-08-27 | 2011-02-16 | 浙江大学 | Preparation method and discrimination method of coating seeds with anti-counterfeit function |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5501053B2 (en) * | 2010-03-25 | 2014-05-21 | 株式会社サカタのタネ | Method for producing effective microorganism-coated seeds requiring a decompression step |
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- 2015-11-20 CN CN201510805038.0A patent/CN105379470B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101322455A (en) * | 2008-07-28 | 2008-12-17 | 李海涛 | Compound aminoacid seed afterbirth substrate for treating sandy wasteland |
| CN101403694A (en) * | 2008-10-31 | 2009-04-08 | 浙江大学 | Fluorescence anti-counterfeit and detection method for pellet seed |
| CN101971727A (en) * | 2010-08-27 | 2011-02-16 | 浙江大学 | Preparation method and discrimination method of coating seeds with anti-counterfeit function |
Non-Patent Citations (2)
| Title |
|---|
| 种子包衣技术的应用与发展;吕伟 等;《天津农林科技》;19971231(第4期);第39-41页 * |
| 薄层色谱法对几种氨基酸鉴定实验的改进;张秋霞 等;《金华职业技术学院学报》;20011231(第3期);第45-46页 * |
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