CN108949578A - A kind of bolete liquid spawn culture medium and preparation method thereof - Google Patents

A kind of bolete liquid spawn culture medium and preparation method thereof Download PDF

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CN108949578A
CN108949578A CN201710346729.8A CN201710346729A CN108949578A CN 108949578 A CN108949578 A CN 108949578A CN 201710346729 A CN201710346729 A CN 201710346729A CN 108949578 A CN108949578 A CN 108949578A
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张胜友
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Abstract

The present invention relates to a kind of bolete liquid spawn culture mediums and preparation method thereof, it is made by the raw material of following parts by weight: 18~24 parts of glucose, 5~8 parts of yeast powder, 2~4 parts of soybean powder, KH2PO40.2~0.6 part, MgSO4·H20.25~0.65 part of O, 15~23 parts of fertile soil, 40~55 parts of soft pin, 1200~1500 parts of water.The present invention uses potato, sucrose, agar, MgSO4、KH2PO4、H2The raw materials such as O prepare bacterium culture medium according to a certain percentage, are particularly suitable for the preparation of yellowish-brown bolete strain and mycelial Submerged fermentation.Culture medium prepared by the present invention can make Boletus impolitus mycelial growth rate fast, not will form former base between growth period of hypha, while providing the high excellent parent species of yield for the artificial cultivation of Boletus impolitus fructification；It can be formed within mycelium pellet 3~5 days, Boletus impolitus yield increases by 20~30% than traditional mother culture media.

Description

A kind of bolete liquid spawn culture medium and preparation method thereof
Technical field
The present invention relates to liquid fungus strain culture medium field, a kind of bolete liquid spawn culture medium and its system are referred in particular to Preparation Method.
Background technique
Beef liver mushroom is the general designation of the fungies such as Boletaceae and pinecone Boletaceae, wherein except a small number of kinds are toxic or taste Bitter and cannot eat outer, most of kind is edible.Yunnan Province's beef liver mushroom is resourceful, mainly there is yellow, white, black, red ox Liver bacterium.Bolete because fleshy hypertrophy, gain the name like beef liver by pole, is rare rare wild edible fungus, is one of " four big bacterium kings ", For the fructification of Boletaceae fungi Boletus speciosus, Boletus queletii Schulzer. Hedwigia, Boletus regius, boletus satanas.Suillus albidipes (Peck) Sing taste It is delicious, it is full of nutrition.The bacterium thallus is larger, and meat is plump, and handle is sturdy, and food flavor is tasty and delicious, full of nutrition, is a kind of worldwide Name edible mushroom.Also there is the habit of edible Suillus albidipes (Peck) Sing extensively in every Western Europe country, and in addition to fresh cook, most of chip drying adds Work is at various inner wrappings, and for preparing soup stock or being made into soy sauce medicinal extract, it is edible that salted product are also made.
Bolete is rich in the minerals such as protein, carbohydrate, vitamin and calcium, phosphorus, iron；Bolete is rare bacterium Class, fragrance is unique, full of nutrition, has the function of preventing and curing diseases, keep fit and healthy, especially has good curative effect to diabetes.In addition, Its water extract has resistance inhibitor action to the growth of small white mouse sarcoma S-180, and the inhibiting rate to sarcoma S-180 is 100%, to Emhorn abdomen The inhibiting rate of water cancer is 90%, while being that China is exported to the famous edible of America and Europe there are also resisiting influenza virus, the anti-effect to cure cold Bacterium.The bacterium thallus is larger, and meat is plump, and handle is sturdy, and food flavor is tasty and delicious, full of nutrition, is a kind of worldwide famous edible mushroom.According to It analyzes, 20.2g containing protein, carbohydrate 64.2g in 100g dry product bolete, 338 kilocalories of heat, ash content 4.0g, Ca23mg, P500mg, Fe50mg, the riboflavin 3.68mg. bacterium have heat-clearing solution tired, blood-nourishing and in, wind-evil dispelling and cold-evil expelling, relaxing muscles and tendons and Blood, qi-restoratives are refreshed oneself and other effects, are one of the raw materials of Chinese patent drug " SHUJIN WAN ".It is gynaecology's good medicine again, women leukorrhea disease and not can be controlled Pregnant disease.Bolete contains 8 kinds of amino acid needed by human, also containing alkaloids such as gland fat purine, choline and putrescine.Treat waist-leg pain Bitterly, numb in every limb, tic of limbs, in addition, there are also resisiting influenza virus, the anti-effect to cure cold.It can be seen that cepe really woods Multiple functional in middle mushroom, food medicine dual-purpose treasure.It is micro- that immunity of organisms, improvement body can be remarkably reinforced in frequent edible bolete Circulation.
But the artificial cultivation of bolete is also highly difficult at present, and during artificial cultivation, mycelia slow growth is grown in mycelia Stage former base easy to form influences accumulation of the mycelium to nutrition, leads to that fructification cannot be normally formed.Bolete Mother culture Base each component type is relatively more, selects to be suitble to the raw material of bolete growth difficult；And the micro variation of Formulation Ingredients dosage can be led Cause the difference of bolete growth course.Though the bolete mycelium of traditional mother culture media culture can obtain son in culture material Entity, but its parent species mycelium is difficult to cover with test tube slant, just will form bolete former base in 5~8d of growth, thus shadow The later normal growth of bolete is rung, and the fruiting body yield of compost output is low.So suitable Boletus impolitus growth Boletus impolitus mother culture media is required at present.
Summary of the invention
The purpose of the present invention is being improved and being innovated for disadvantage present in background technique and problem, a kind of ox is provided Liver bacteria liquid bacterium culture medium, this culture medium can make the growth of bolete mycelia fast, not will form former base between growth period of hypha, The high excellent parent species of yield are provided simultaneously for the artificial cultivation of fructification.
It is a further object of the present invention to provide the preparation methods of the culture medium.
Culture medium of the present invention is made by the raw material of following parts by weight: 18~24 parts of glucose, 5~8 parts of yeast powder, Huang 2~4 parts of bean powder, KH2PO4O.2~0.6 part, MgSO4·H2O O.25~0.65 part, 15~23 parts of fertile soil, soft pin 40~ 55 parts, 1200~1500 parts of water.
Culture medium of the present invention can also be made by the raw material of following parts by weight: 18~20 parts of corn flour, sucrose 4~6 Part, 3~3.5 parts of glucose, KH2PO4 O.5~0.8 part, MgSO4·H2O O.4~0.6 part, 1~2 part of peptone, soybean powder 3~4 parts, 5~8 parts of yeast powder, 18~22 parts of fertile soil, 46~55 parts of soft pin, 2000~2400 parts of water.
The preparation method of above two culture medium weighs each raw material for standby the following steps are included: 1) by institute's expense, cleans Tankage；2) the load weighted raw material in addition to fertile soil, soft pin is put into container, is added 200~300 parts of water, stirs It mixes uniformly, steam heating is boiled 20~30 minutes, is stirred when boiling, and standing 5~placed after ten minutes with 4 layers of filtered through gauze is standby With；3) load weighted fertile soil is put into beaker, 200~250 parts of water is added, boil extraction 15~22 minutes, filtered, will filter Liquid is added in container；4) load weighted soft pin is put into beaker, 200~250 parts of water is added, boil 15~20 points of extraction Clock takes leaching liquor to be added in container；5) by step 2,3), 4) made from liquid mix and stir evenly, heating 3~5 Minute, 90~100 DEG C of temperature；6) adding water constant volume, and be dispensed into triangular flask, every bottle of 500ml has dispensed silica gel plug in rear plug, It the additional non-woven fabrics of brown paper and is tied up with rope tight on cover, is then charged into high-pressure sterilizing pot；121 DEG C of sterilising temp, pressure 105KPa, Time 20~40 minutes to get Boletus impolitus mother culture media.
Culture medium of the present invention can also be made by the raw material of following parts by weight: 20~25 parts of peeling potatoes, grape Sugared 16~22 parts, 5~8 parts of yeast powder, 3~4 parts of soybean powder, KH2PO4O.2~0.5 part, MgSO4·H2O is O.30~0.60 Part, 4~8 parts of peptone, 18~25 parts of fertile soil, 35~50 parts of soft pin, 1800~2000 parts of water.
The preparation method of above-mentioned culture medium weighs each raw material for standby, cleans container the following steps are included: 1) by institute's expense Equipment；2) by the peeled potatoes weighed up slice or item, add 200~300 parts of water to heat on electromagnetic oven and boil 20 minutes, make Potato is thoroughly well cooked but not mushy, then takes supernatant with 4 layers of filtered through gauze；Then other originals in addition to fertile soil, soft pin are added Material stirs evenly, and steam heating is boiled 20~30 minutes, stirs when boiling, standing 5~put after ten minutes with 4 layers of filtered through gauze It sets, it is spare；3) load weighted fertile soil is put into beaker, 200~250 parts of water is added, boil extraction 15~22 minutes, mistake Filter, filtrate is added in container；4) load weighted soft pin is put into beaker, 200~250 parts of water is added, boil extraction 15 ~20 minutes, leaching liquor is taken to be added in container；5) by step 2,3), 4) made from liquid mix and stir evenly, add Heat 3~5 minutes, 90~100 DEG C of temperature；6) add water constant volume, and be dispensed into triangular flask, every bottle of 500ml has dispensed silicon in rear plug Rubber plug the additional non-woven fabrics of brown paper and is tied up tight on cover with rope, is then charged into high-pressure sterilizing pot；121 DEG C of sterilising temp, pressure 105KPa, the time 20~40 minutes to get Boletus impolitus mother culture media.
Glucose of the present invention is medical glucose.
It is best that soft pin of the present invention and fertile soil, which are picked up from wild Boletus impolitus growing location,.Other places Soft pin and fertile soil can also be applied.
Glucose used is pharmaceutical glucose in the present invention, and glucose provides carbon source needed for Boletus impolitus is grown.Ferment Female powder makes microorganism that the speed of various nutrition and more efficient be absorbed and utilized.Soybean powder provides nitrogen needed for Boletus impolitus silk is grown Source, KH2PO4With MgSO4·H2O provides the minerals such as phosphorus, potassium and magnesium required for Boletus impolitus silk is grown；Fertile soil increases The nutrition of bolete original growing location, makes the mycelium of Boletus impolitus adapt to artificial medium, accelerates its speed of growth. A large amount of minerals are had accumulated during biological cycle humous, based on calcium, potassium, there are also sulphur, phosphorus etc..Boletus impolitus be with The Applying Ectomycorrhizal Fungi of pine tree symbiosis adds soft pin composition in liquid spawn culture medium, mainly makes up artificial medium The element in pine tree that the Boletus impolitus lacked needs.Pine needle rich in carbohydrate, crude protein, crude fat, a variety of amino acid and Lot of trace mine element, multivitamin, biological flavone substance, essential oil, chlorophyll, unsaturated fatty acid, enzyme and coenzyme etc. Active material.Boletus impolitus liquid spawn mother culture media in the present invention cultivates the mycelial growth speed of Boletus impolitus Degree is fast, and bacterium ball is uniform in size；It is formed within mycelium pellet 3~4 days, bolete yield increases by 25~35% than traditional mother culture media.
Advantages of the present invention and the utility model has the advantages that
The present invention uses potato, sucrose, agar, MgSO4、KH2PO4、H2The raw materials such as O prepare Spawn incubation according to a certain percentage Base and liquid spawn culture medium, culture yellowish-brown bolete fructification amputated body parts prepare strain, using fluid nutrient medium according to Liquid cultivating method carries out mycelial Submerged fermentation.Culture medium provided in the present invention is particularly suitable for yellowish-brown beef liver The preparation of bacterium strain and mycelial Submerged fermentation.Using the cultural method, yellowish-brown bolete mycelium can be quickly carried out Fermented and cultured.
Boletus impolitus mycelial growth rate is fast in the present invention, not will form former base between growth period of hypha, while being Huang The artificial cultivation of brown bolete fructification provides the high excellent parent species of yield；It can be formed within mycelium pellet 3~5 days, yellowish-brown beef liver Bacterium yield increases by 20~30% than traditional mother culture media.
Specific embodiment
It is of the invention for ease of understanding, several embodiments of the present invention are shown below.But the present invention can be with many not With form realize, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is makes to this The disclosure of invention is more thorough and comprehensive.
Unless otherwise defined, the skill of all technical and scientific terms and technical field of the invention used herein The normally understood meaning of art personnel is identical.Term used in the description is intended merely to describe specifically to implement purpose, is not It is designed to limit the invention.
Embodiment 1:
1, by medicinal glucose 20g, yeast powder 6.5g, soybean powder 3g, KH2PO4 O.5g、MgSO4·H2O.5g, O is put into 1000ml measuring cup is added 300ml water, stirs evenly, and steam heating is boiled 20 minutes, is stirred when boiling, and stands after five minutes with 4 Layer filtered through gauze, is placed spare；
2, fertile soil 20g is put into beaker and 200ml boiling boiling is added extraction 20 minutes, filtering, filtrate is added to 1000ml measuring cup In, it is spare.
3,200ml boiling boiling is added extraction 15 minutes in soft pin 50g, leaching liquor is taken to pour into 1000ml measuring cup, it is spare.
4,1-3 step liquid obtained is mixed and is stirred evenly, heated 3 minutes, 90 DEG C of temperature.
5, Jia Shui is settled to 1000ml, is dispensed into triangular flask, every bottle of 500ml, has dispensed silica gel plug in rear plug, on cover The additional non-woven fabrics of brown paper simultaneously ties up tight, conventional high temperature high pressure sterilization with rope, and 121 DEG C of temperature, pressure 105KPa, 30 minutes i.e. Obtain Boletus impolitus liquid spawn mother culture media.
Embodiment 2:
1, by medicinal glucose 18g, yeast powder 5g, soybean powder 2g, KH2PO4 O.2g、MgSO4·H2O.25g, O is put into container In, 220ml water is added, stirs evenly, steam heating is boiled 25 minutes, is stirred when boiling, with 4 layers of gauze mistake after standing 8 minutes Filter is placed spare；
2, fertile soil 15g is put into beaker and 240ml boiling boiling is added extraction 20 minutes, filtering, filtrate is added in container, standby With.
3,250ml boiling boiling is added extraction 20 minutes in soft pin 40g, leaching liquor is taken to pour into container, it is spare.
4,1-3 step liquid obtained is mixed and is stirred evenly, heated 5 minutes, 100 DEG C of temperature.
5, Jia Shui is settled to 1000ml, is dispensed into triangular flask, every bottle of 500ml, has dispensed silica gel plug in rear plug, on cover The additional non-woven fabrics of brown paper simultaneously ties up tight, conventional high temperature high pressure sterilization with rope, and 121 DEG C of temperature, pressure 105KPa, 35 minutes i.e. Obtain Boletus impolitus liquid spawn mother culture media.
Embodiment 3:
1, by medicinal glucose 24g, yeast powder 8g, soybean powder 4g, KH2PO4 O.6g、MgSO4·H2O.65g, O is put into container In, 300ml water is added, stirs evenly, steam heating is boiled 30 minutes, is stirred when boiling, and is stood after ten minutes with 4 layers of gauze mistake Filter is placed spare；
2, fertile soil 23g is put into beaker and 250ml boiling boiling is added extraction 22 minutes, filtering, filtrate is added in container, standby With.
3,250ml boiling boiling is added extraction 20 minutes in soft pin 55g, leaching liquor is taken to pour into container, it is spare.
4,1-3 step liquid obtained is mixed and is stirred evenly, heated 5 minutes, 100 DEG C of temperature.
5, Jia Shui is settled to 1500ml, is dispensed into triangular flask, every bottle of 500ml, has dispensed silica gel plug in rear plug, on cover The additional non-woven fabrics of brown paper simultaneously ties up tight, conventional high temperature high pressure sterilization with rope, and 121 DEG C of temperature, pressure 105KPa, 40 minutes i.e. Obtain Boletus impolitus liquid spawn mother culture media.
Embodiment 4:
1, peeled potatoes 20g is sliced, and is added water 200ml to heat on electromagnetic oven and is boiled 20 minutes, keeps potato thoroughly well cooked but not mushy, then Supernatant is taken with 4 layers of filtered through gauze.
2, by glucose 16g, yeast powder 5g, soybean powder 3g, KH2PO4 0.2g、MgSO4·H2O 0.3g, peptone 4g add Enter and stirred evenly in upper step clear liquid, steam heating is boiled 20 minutes, is stirred when boiling, and standing is put with 4 layers of filtered through gauze after five minutes It sets, it is spare.
3, fertile soil 18g is put into beaker, 200ml water is added, boil extraction 15 minutes, filtrate is added to appearance by filtering It is spare in device.
4, soft pin 35g is put into beaker, 200ml water is added, boiled extraction 15 minutes, leaching liquor is taken to be added to container In, it is spare.
5,2-4 step liquid obtained is mixed and is stirred evenly, heated 3 minutes, 90 DEG C of temperature.
6, water 1200ml constant volume is added, and is dispensed into triangular flask, every bottle of 500ml, has dispensed silica gel plug in rear plug, on cover The additional non-woven fabrics of brown paper is simultaneously tied up tightly with rope, and high-pressure sterilizing pot is then charged into；121 DEG C of sterilising temp, pressure 105KPa, the time 30 minutes to get Boletus impolitus mother culture media.
Embodiment 5:
1, peeled potatoes 25g is cut, and is added water 300ml to heat on electromagnetic oven and is boiled 20 minutes, keeps potato thoroughly well cooked but not mushy, then Supernatant is taken with 4 layers of filtered through gauze.
2, by glucose 22g, yeast powder 8g, soybean powder 4g, KH2PO4 0.5g、MgSO4·H2O 0.6g, peptone 8g add Enter and stirred evenly in upper step clear liquid, steam heating is boiled 30 minutes, is stirred when boiling, and is stood after ten minutes with 4 layers of filtered through gauze It places, it is spare.
3, fertile soil 25g is put into beaker, 250 parts of water is added, boil extraction 20 minutes, filtrate is added to appearance by filtering It is spare in device.
4, soft pin 50g is put into beaker, 250 parts of water is added, boiled extraction 20 minutes, leaching liquor is taken to be added to container In, it is spare.
5,2-4 step liquid obtained is mixed and is stirred evenly, heated 5 minutes, 100 DEG C of temperature.
6, water 1200ml constant volume is added, and is dispensed into triangular flask, every bottle of 500ml, has dispensed silica gel plug in rear plug, on cover The additional non-woven fabrics of brown paper is simultaneously tied up tightly with rope, and high-pressure sterilizing pot is then charged into；121 DEG C of sterilising temp, pressure 105KPa, the time 35 minutes to get Boletus impolitus mother culture media.
Embodiment 6:
1, peeled potatoes 22g is cut, and is added water 250ml to heat on electromagnetic oven and is boiled 20 minutes, keeps potato thoroughly well cooked but not mushy, then Supernatant is taken with 4 layers of filtered through gauze.
2, by glucose 20g, yeast powder 6g, soybean powder 3.5g, KH2PO4 0.3g、MgSO4·H2O 0.5g, peptone 6g It walks in addition and is stirred evenly in clear liquid, steam heating is boiled 30 minutes, is stirred when boiling, with 4 layers of filtered through gauze after standing 6 minutes It places, it is spare.
3, fertile soil 20g is put into beaker, 200 parts of water is added, boil extraction 20 minutes, filtrate is added to appearance by filtering It is spare in device.
4, soft pin 40g is put into beaker, 250 parts of water is added, boiled extraction 15 minutes, leaching liquor is taken to be added to container In, it is spare.
5,2-4 step liquid obtained is mixed and is stirred evenly, heated 5 minutes, 100 DEG C of temperature.
6, water 1200ml constant volume is added, and is dispensed into triangular flask, every bottle of 500ml, has dispensed silica gel plug in rear plug, on cover The additional non-woven fabrics of brown paper is simultaneously tied up tightly with rope, and high-pressure sterilizing pot is then charged into；121 DEG C of sterilising temp, pressure 105KPa, the time 40 minutes to get Boletus impolitus mother culture media.
Embodiment 7:
1, by corn flour 18g, sucrose 4g, medicinal glucose 3g, KH2PO4 O.5g、MgSO4·H2O O.4g, peptone 1g, Huang Bean powder 3g, yeast powder 5g are put into 1000ml measuring cup, and 200ml water is added, stirs evenly, and steam heating is boiled 20 minutes, when boiling Stirring stands after five minutes with 4 layers of filtered through gauze, places spare；
2, fertile soil 18g is put into beaker and 200ml boiling boiling is added extraction 20 minutes, filtering, filtrate is added to 1000ml measuring cup In, it is spare.
3,200ml boiling boiling is added extraction 15 minutes in soft pin 46g, leaching liquor is taken to pour into 1000ml measuring cup, it is spare.
4,1-3 step liquid obtained is mixed and is stirred evenly, heated 3 minutes, 100 DEG C of temperature.
5, add water constant volume, be dispensed into triangular flask, every bottle of 500ml, silica gel plug in rear plug dispensed, on cover outside brown paper Add non-woven fabrics and tie up tight, conventional high temperature high pressure sterilization with rope, 121 DEG C of temperature, pressure 105KPa, 25 minutes up to yellowish-brown beef liver Bacteria liquid strain mother culture media.
Embodiment 8:
1, by corn flour 20g, sucrose 6g, medicinal glucose 3.5g, KH2PO4 O.8g、MgSO4·H2O O.6g, peptone 2g, Soybean powder 4g, yeast powder 8g are put into container, and 300ml water is added, stirs evenly, and steam heating is boiled 30 minutes, are stirred when boiling It mixes, stands after ten minutes with 4 layers of filtered through gauze, place spare；
2, fertile soil 22g is put into beaker and 250ml boiling boiling is added extraction 22 minutes, filtering, filtrate is added in container, standby With.
3,250ml boiling boiling is added extraction 20 minutes in soft pin 55g, leaching liquor is taken to pour into container, it is spare.
4,1-3 step liquid obtained is mixed and is stirred evenly, heated 5 minutes, 90 DEG C of temperature.
5, add water constant volume, be dispensed into triangular flask, every bottle of 500ml, silica gel plug in rear plug dispensed, on cover outside brown paper Add non-woven fabrics and tie up tight, conventional high temperature high pressure sterilization with rope, 121 DEG C of temperature, pressure 105KPa, 30 minutes up to yellowish-brown beef liver Bacteria liquid strain mother culture media.
Embodiment 9:
1, by corn flour 18g, sucrose 5g, medicinal glucose 3.5g, KH2PO4 O.6g、MgSO4·H2O O.5g, peptone 1.5g, soybean powder 4g, yeast powder 7g are put into container, and 250ml water is added, stirs evenly, and steam heating is boiled 25 minutes, while boiling Side stirring is placed spare with 4 layers of filtered through gauze after standing 6 minutes；
2, fertile soil 20g is put into beaker and 200ml boiling boiling is added extraction 20 minutes, filtering, filtrate is added in container, standby With.
3,250ml boiling boiling is added extraction 15 minutes in soft pin 50g, leaching liquor is taken to pour into container, it is spare.
4,1-3 step liquid obtained is mixed and is stirred evenly, heated 5 minutes, 100 DEG C of temperature.
5, add water constant volume, be dispensed into triangular flask, every bottle of 500ml, silica gel plug in rear plug dispensed, on cover outside brown paper Add non-woven fabrics and tie up tight, conventional high temperature high pressure sterilization with rope, 121 DEG C of temperature, pressure 105KPa, 35 minutes up to yellowish-brown beef liver Bacteria liquid strain mother culture media.
The cultural method of culture medium of the present invention: Boletus impolitus is implanted into mother culture media, static in 28 DEG C of incubators Culture 24 hours, such as Boletus impolitus, cepe or the brown bolete of ash.
Culture medium of the present invention is used as Boletus impolitus parent species liquid spawn culture medium, is aseptically accessed yellowish-brown Bolete test tube stock stands 24 hours, starts to start bottle swingging machine shaking flask under 28 DEG C of environment temperatures, initially forms mycelia within 3 days Ball, mycelium pellet is grown within 5 days, can be inoculated with.
Embodiment of the present invention is only the description carried out to the preferred embodiment of the present invention, not to the present invention Conception and scope is defined, and under the premise of not departing from design philosophy of the present invention, engineers and technicians are to this hair in this field The all variations and modifications that bright technical solution is made should all fall into protection scope of the present invention, the claimed skill of the present invention Art content, is all described in the claims.

Claims (7)

1. a kind of bolete liquid spawn culture medium, it is characterised in that it is made by the raw material of following parts by weight:
18~24 parts of glucose, 5~8 parts of yeast powder, 2~4 parts of soybean powder, KH2PO4O.2~0.6 part, MgSO4·H2O O.25 ~0.65 part, 15~23 parts of fertile soil, 40~55 parts of soft pin, 1200~1500 parts of water.
2. a kind of bolete liquid spawn culture medium, it is characterised in that it is made by the raw material of following parts by weight:
20~25 parts of peeling potatoes, 16~22 parts of glucose, 5~8 parts of yeast powder, 3~4 parts of soybean powder, KH2PO4O.2~ 0.5 part, MgSO4·H2O O.30~0.60 part, 4~8 parts of peptone, 18~25 parts of fertile soil, 35~50 parts of soft pin, water 1800~2000 parts.
3. a kind of bolete liquid spawn culture medium, it is characterised in that it is made by the raw material of following parts by weight:
18~20 parts of corn flour, 4~6 parts of sucrose, 3~3.5 parts of glucose, KH2PO4 O.5~0.8 part, MgSO4·H2O O.4 ~0.6 part, 1~2 part of peptone, 3~4 parts of soybean powder, 5~8 parts of yeast powder, 18~22 parts of fertile soil, soft pin 46~55 Part, 2000~2400 parts of water.
4. according to claim 1 to a kind of bolete liquid spawn culture medium described in one of 3, it is characterised in that the grape Sugar is medical glucose.
5. according to claim 1 to a kind of bolete liquid spawn culture medium described in one of 3, it is characterised in that described is dry and soft Needle and fertile soil are picked up from wild Boletus impolitus growing location.
6. a kind of preparation method of bolete liquid spawn culture medium according to claim 1 or 3, it is characterised in that including Following steps:
1) institute's expense is pressed, each raw material for standby is weighed, cleans tankage；
2) the load weighted raw material in addition to fertile soil, soft pin is put into container, is added 200~300 parts of water, stirring is equal Even, steam heating is boiled 20~30 minutes, is stirred when boiling, and standing 5~placed after ten minutes with 4 layers of filtered through gauze is spare；
3) load weighted fertile soil is put into beaker, 200~250 parts of water is added, boil extraction 15~22 minutes, filtered, it will Filtrate is added in container；
4) load weighted soft pin is put into beaker, 200~250 parts of water is added, boils extraction 15~20 minutes, takes leaching liquor It is added in container；
5) by step 2,3), 4) made from liquid mix and stir evenly, heat 3~5 minutes, 90~100 DEG C of temperature；
6) add water constant volume, and be dispensed into triangular flask, every bottle of 500ml, dispense silica gel plug in rear plug, the additional nothing of brown paper on cover Woven fabric is simultaneously tied up tightly with rope, and high-pressure sterilizing pot is then charged into；121 DEG C of sterilising temp, pressure 105KPa, the time 20~40 minutes, Up to Boletus impolitus mother culture media.
7. a kind of preparation method of bolete liquid spawn culture medium according to claim 2, it is characterised in that including with Lower step:
1) institute's expense is pressed, each raw material for standby is weighed, cleans tankage；
2) by the peeled potatoes weighed up slice or item, add 200~300 parts of water to heat on electromagnetic oven and boil 20 minutes, make Potato is thoroughly well cooked but not mushy, then takes supernatant with 4 layers of filtered through gauze；Then other originals in addition to fertile soil, soft pin are added Material stirs evenly, and steam heating is boiled 20~30 minutes, stirs when boiling, standing 5~put after ten minutes with 4 layers of filtered through gauze It sets, it is spare；
3) load weighted fertile soil is put into beaker, 200~250 parts of water is added, boil extraction 15~22 minutes, filtered, it will Filtrate is added in container；
4) load weighted soft pin is put into beaker, 200~250 parts of water is added, boils extraction 15~20 minutes, takes leaching liquor It is added in container；
5) by step 2,3), 4) made from liquid mix and stir evenly, heat 3~5 minutes, 90~100 DEG C of temperature；
6) add water constant volume, and be dispensed into triangular flask, every bottle of 500ml, dispense silica gel plug in rear plug, the additional nothing of brown paper on cover Woven fabric is simultaneously tied up tightly with rope, and high-pressure sterilizing pot is then charged into；121 DEG C of sterilising temp, pressure 105KPa, the time 20~40 minutes, Up to Boletus impolitus mother culture media.
CN201710346729.8A 2017-05-17 2017-05-17 A kind of bolete liquid spawn culture medium and preparation method thereof Pending CN108949578A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009240275A (en) * 2008-03-31 2009-10-22 Naris Cosmetics Co Ltd Liquid culture medium for boletaceae suillus basidiomycete
JP2009240276A (en) * 2008-03-31 2009-10-22 Naris Cosmetics Co Ltd Liquid culture medium for boletaceae suillus basidiomycete
CN103011935A (en) * 2012-12-20 2013-04-03 中国科学院、水利部成都山地灾害与环境研究所 Boletus mother culture medium and preparation method thereof
CN109097288A (en) * 2018-08-02 2018-12-28 周茂 A kind of fluid nutrient medium, the preparation method of Phlebopus portentosus batch production liquid spawn and the purposes of the fluid nutrient medium

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009240275A (en) * 2008-03-31 2009-10-22 Naris Cosmetics Co Ltd Liquid culture medium for boletaceae suillus basidiomycete
JP2009240276A (en) * 2008-03-31 2009-10-22 Naris Cosmetics Co Ltd Liquid culture medium for boletaceae suillus basidiomycete
CN103011935A (en) * 2012-12-20 2013-04-03 中国科学院、水利部成都山地灾害与环境研究所 Boletus mother culture medium and preparation method thereof
CN109097288A (en) * 2018-08-02 2018-12-28 周茂 A kind of fluid nutrient medium, the preparation method of Phlebopus portentosus batch production liquid spawn and the purposes of the fluid nutrient medium

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