CN103011935B - Boletus mother culture medium and preparation method thereof - Google Patents
Boletus mother culture medium and preparation method thereof Download PDFInfo
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- CN103011935B CN103011935B CN201210557661.5A CN201210557661A CN103011935B CN 103011935 B CN103011935 B CN 103011935B CN 201210557661 A CN201210557661 A CN 201210557661A CN 103011935 B CN103011935 B CN 103011935B
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Abstract
The invention relates to the field of fungus culture media, in particular to a boletus mother culture medium and a preparation method thereof. The boletus mother culture medium comprises the following components in part by weight: 18-24 parts of glucose, 5-8 parts of yeast powder, 2-4 parts of soybean meal, 40.2-0.6 parts of KH2PO, 0.25-0.65 parts of MgSO4.H2O, 15-23 parts of humus, 40-55 parts of dry pine needles, 14-26 parts of agar and 800-1500 parts of water. The boletus mycelium is high in growth speed; during growth of the mycelium, a primordium is not formed; an excellent mother with high yield is provided for artificial cultivation of a sporocarp; on the 14-16th day, the mycelium can grow fully on a test tube slant with the length of 5-8cm; and compared with the conventional mother culture medium, the boletus mother culture medium can increase the yield of the boletus by 20-30%.
Description
Technical field
The invention belongs to fungi culture medium field, relate in particular to a kind of bolete mother culture media and preparation method thereof.
Background technology
Beef liver mushroom is the general designation of the fungies such as Boletaceae and pinecone Boletaceae, wherein except minority kind is poisonous or bitter and can not eating, and the equal edible of most of kind.Some bolete delicious flavour, nutritious, as King Boletus, yellowish-brown bolete and grey brown bolete etc.The general thalline of edible bolete is larger, and meat is plump, and handle is sturdy, and food flavor is fragrant and sweet good to eat, nutritious, is the worldwide famous edible mushrooms of a class.
The mineral substance such as bolete rich in proteins, carbohydrate, VITAMIN and calcium, phosphorus, iron.
Bolete is precious mushroom, and some bolete kind fragrance is unique, nutritious, and some bolete kind has the function of preventing and curing diseases, improving the health, and especially diabetes is had to good curative effect.In addition, its aqueous extract of the bolete of some kind has resistance inhibitor action to the growth of small white mouse sarcoma S-180, to the inhibiting rate of sarcoma S-180, is 100%, to the inhibiting rate of ehrlich carcinoma, is 90%, also have resisiting influenza virus, the anti-effect curing cold, be that American-European famous edible mushrooms is found a good sale in by China simultaneously.
According to one's analysis, can be containing 20.2 grams, protein in 100 grams of bolete dry products, 64.2 grams, carbohydrate, 338 kilocalories of heats, 4.0 grams of ash contents, Ca23 milligram, P500 milligram, Fe50 milligram, 3.68 milligrams, riboflavin. bolete have heat-clearing solution be tired of, nourish blood and in, wind-evil dispelling and cold-evil expelling, relaxing muscles and tendons and blood, the qi-restoratives effect such as refresh oneself, be one of raw material of Chinese patent medicine " SHUJIN WAN "; You Shi gynaecology good medicine, can control women leukorrhea disease and Infertility.
In addition, also have resisiting influenza virus, the anti-effect curing cold.Visible bolete is the treasure of multiple functional in numerous mushrooms, food medicine dual-purpose really.Often edible bolete obviously enhancing body immunizing power, improve body microcirculation.
But the artificial culture of bolete is very difficult, in culturing process, mycelia produces slow, at vegetative stage, easily forms former base, affects the accumulation of mycelium to nutrition, causes normally forming sporophore.Mother culture media each component kind is many, selects the raw material difficulty of proper growth; And the trace variation of formula composition consumption can cause the difference of bolete process of growth.Though the bolete mycelium that tradition mother culture media is cultivated can obtain sporophore in planting material, but its female mycelium of planting is difficult to cover with test tube slant, what grow, within 4-7 days, will form former base, and affect the later normal growth of bolete, and the fruiting body yield of culture material output is low.
So the bolete mother culture media of suitable bolete growth is required at present.
Summary of the invention
For described problem, the object of this invention is to provide a kind of bolete mother culture media and preparation method.This bolete mycelial growth is fast, during mycelial growth, can not form former base, and the artificial culture for sporophore provides good female kind that output is high simultaneously.
Realizing the object of the invention technical scheme is:
A bolete mother culture media, metering by weight includes following component:
Glucose 18-24 part
Yeast powder 5-8 part
Analysis for soybean powder 2-4 part
KH
2pO
40.2-0.6 part
MgSO
4h
2o 0.25-0.65 part
Vegetable mould 15-23 part
Dry and soft pin 40-55 part
Agar 14-26 part
Water 800-1500 part
As preferred version, in the present invention, the preferable amount of each component is:
Glucose 19-22 part
Yeast powder 6-7.5 part
Analysis for soybean powder 2.5-3.5 part
KH
2pO
40.3-0.53 part
MgSO
4h
2o 0.4-0.6 part
Vegetable mould 18-21 part
Dry and soft pin 48-52 part
Agar 16-24 part
Water 900-1300 part
In the present invention, the optimum amount of each component is:
20 parts of glucose
6.5 parts of yeast powders
3 parts of analysis for soybean powder
KH
2pO
40.5 part
MgSO
4h
20.45 part of O
20 parts of vegetable mould
50 parts, dry and soft pin
20 parts, agar
1000 parts, water
The method of preparing bolete mother culture media of the present invention, according to the following steps operation:
Step 1, raw material weighing, cleaning vessel equipment;
Step 2, by the 18-24 part glucose weighing up, 5-8 part yeast powder, 2-4 part analysis for soybean powder, 0.2-0.6 part
KH
2pO
4, 0.25-0.65 part MgSO
4h
2o, puts into container, adds 200-300 part water, places standby;
Step 3, the 15-23 part vegetable mould weighing up is put into beaker add 200-250 part water boil lixiviate 20 minutes, filter, filtrate is added in container;
Step 4, the dry and soft pin of 40-55 part is put into beaker add 200-250 part water boil lixiviate 15 minutes, get vat liquor and join in same container;
Step 5, by the mixed solution heating 1-3 minute in said vesse, make temperature reach 90-100 ℃; Add again 14-26 part agar, after heating and dissolving completely to agar for 2-3 minute, add water constant volume, minute install in test tube;
Step 6, by the material in test tube under 120 ℃ of temperature, pressure 101.33 KPa, with sterilizing in 20-30 minute, obtain bolete mother culture media.
Described glucose is pharmaceutical glucose.Glucose provides the bolete required carbon source of growing.
It is higher that yeast powder makes microorganism absorb speed and the efficiency of various nutrition.
Analysis for soybean powder provides beef liver mycelial growth required nitrogenous source, KH
2pO
4with MgSO
4h
2o provides the mineral substance such as the needed phosphorus potassium of beef liver mycelial growth and magnesium; Vegetable mould increases the former nutrition vegetatively of bolete, makes the mycelia physical efficiency of bolete adapt to artificial medium, accelerates its speed of growth.In biological cycle process humous, accumulate a large amount of mineral substance, take calcium, potassium as main, also had sulphur, phosphorus etc.
Bolete is and the Applying Ectomycorrhizal Fungi of pine tree symbiosis, adds dry and soft pin composition in substratum, is mainly the element making up in the pine tree of the bolete needs that artificial medium lacks.Pine needle is rich in carbohydrate, crude protein, crude fat, multiple amino acids and lot of trace ore deposit element, multivitamin, bioflavonoid material, essential oil, chlorophyll, unsaturated fatty acids, enzyme and coenzyme isoreactivity material.
Agar dissolves the cooling solidifying liq that is, and makes substratum become solid state, and convenient operation is convenient to switching and is gone down to posterity, also convenient preservation.
Mother culture media in the present invention, the Mycelium growth rate of cultivating bolete is fast, and early growth period does not form former base; Within mycelia 14-16 days, can cover with the test tube slant of 5-8 centimetre, in test tube, not form former base, bolete output increases 20-30% than traditional mother culture media.
Specific embodiment
Dry and soft pin and vegetable mould pick up from wild Boletus edulis vegetatively below, and the dry and soft pin in other place and vegetable mould are also applicable.
embodiment 1
By the 18-24 part glucose weighing up, 5-8 part yeast powder, 2-4 part analysis for soybean powder, 0.2-0.6 part
KH
2pO
4, 0.25-0.65 part MgSO
4h
2o, puts into container, adds 200-300 part water, places standby;
The 15-23 part vegetable mould weighing up is put into beaker and add 200-250 part water boil lixiviate 15-22 minute, filter, filtrate is added in container; The dry and soft pin of 40-55 part is put into beaker and add 200-250 part water boil lixiviate 15-20 minute, get vat liquor and add in container;
Above material is at vessel in heating 1-3 minute, temperature 90-100 ℃; Add again 14-22 part agar, after heating and dissolving completely to agar for 2-3 minute, add water constant volume, minute install in test tube;
Sterilizing, 120 ℃ of temperature, pressure 101.33 KPa, 30 minutes time obtained bolete mother culture media.
embodiment 2
By the 19-22 part glucose weighing up, 6-7.5 part yeast powder, 2.5-3.5 part analysis for soybean powder, 0.3-0.53 part
KH
2pO
4, 0.4-0.6 part MgSO
4h
2o, puts into container, adds 200-300 part water, places standby; The 18-21 part vegetable mould weighing up is put into beaker and add 200-250 part water boil lixiviate 15-22 minute, filter, filtrate is added in container; The dry and soft pin of 48-52 part is put into beaker and add 200-250 part water boil lixiviate 15-20 minute, get vat liquor and add in container;
Above material is at vessel in heating 1-3 minute, temperature 90-100 ℃; Add again 16-21 part agar, after heating and dissolving completely to agar for 2-3 minute, add water constant volume, minute install in test tube;
Sterilizing, 120 ℃ of temperature, pressure 101.33 KPa, 25 minutes time obtained bolete mother culture media.
embodiment 3
By 20 parts of glucose that weigh up, 6.5 parts of yeast powders, 3 parts of analysis for soybean powder, 0.5 part of KH
2pO
4, 0.45 part of MgSO
4h
2o, puts into container, adds 300 parts of water, places standby; 20 parts of vegetable mould that weigh up are put into beaker and add 200 parts of water boil lixiviate 15-22 minute, filter, filtrate is added in container; 50 parts of dry and soft pins are put into beaker and add 200 parts of water boil lixiviate 15-20 minute, get vat liquor and add in container;
Above material is at vessel in heating 1-3 minute, temperature 90-100 ℃; Add again 20 parts of agar, after heating and dissolving completely to agar for 2-3 minute, add water constant volume, minute install in test tube;
Sterilizing, 120 ℃ of temperature, pressure 101.33 KPa, 30 minutes time obtained bolete mother culture media.
embodiment 4
By 20 grams of the medicinal glucose weighing up, 6.5 grams of yeast powders, 3 grams of KH of analysis for soybean powder
2pO
40.5 gram, MgSO
4h
20.5 gram of O, puts into 1000 ml vol cups, adds 300 ml waters, places standby; The vegetable mould weighing up is put into beaker and add 200 ml waters to boil lixiviate 20 minutes, filter, filtrate is added in 1000 ml vol cups; Add 200 ml waters to boil lixiviate 15 minutes on dry and soft pin, get vat liquor and pour 1000 ml vol cups into;
Above material boils 1-2 minute together, after all medicines dissolve, adds 20 grams of agar, after being heated to agar and dissolving completely, add water and be settled to 1000 milliliters, divide and install in test tube, conventional autoclave sterilization, 120 ℃ of temperature, pressure 101.33 KPa, obtain bolete mother culture media for 30 minutes.
embodiment 5
By 18 parts of glucose that weigh up, 5 parts of yeast powders, 2 parts of analysis for soybean powder, 0.2 part of KH
2pO
4, 0.25 part of MgSO
4h
2o, puts into container, adds 220 parts of water, places standby; 15 parts of vegetable mould that weigh up are put into beaker and add 240 parts of water boil lixiviates 20 minutes, filter, filtrate is added in container; 40 parts of dry and soft pins are put into beaker and add 250 parts of water boil lixiviates 15 minutes, get vat liquor and add in container;
Above material is in vessel in heating 3 minutes, 100 ℃ of temperature; Add again 14 parts of agar, after heating and dissolving completely to agar for 3 minutes, add water constant volume, minute install in test tube;
Sterilizing, 120 ℃ of temperature, pressure 101.33 KPa, 30 minutes time obtained bolete mother culture media.
embodiment 6
By 24 parts of glucose that weigh up, 8 parts of yeast powders, 4 parts of analysis for soybean powder, 0.6 part of KH
2pO
4, 0.65 part of MgSO
4h
2o, puts into container, adds 200-300 part water, places standby; 23 parts of vegetable mould that weigh up are put into beaker and add 200-250 part water boil lixiviate 20 minutes, filter, filtrate is added in container; 55 parts of dry and soft pins are put into beaker and add 50 parts of water boil lixiviates 20 minutes, get vat liquor and add in container;
Above material is in vessel in heating 3 minutes, 100 ℃ of temperature; Add again 22 parts of agar, after heating and dissolving completely to agar for 3 minutes, add water constant volume, minute install in test tube;
Sterilizing, 120 ℃ of temperature, pressure 101.33 KPa, 40 minutes time obtained bolete mother culture media.
embodiment 7
By 19 parts of glucose that weigh up, 6 parts of yeast powders, 2.5 parts of analysis for soybean powder, 0.3 part of KH
2pO
4, 0.4 part of MgSO
4h
2o, puts into container, adds 200 parts of water, places standby; 18 parts of vegetable mould that weigh up are put into beaker and add 250 parts of water boil lixiviates 15 minutes, filter, filtrate is added in container; 48 parts of dry and soft pins are put into beaker and add 200 parts of water boil lixiviates 15 minutes, get vat liquor and add in container;
Above material is in vessel in heating 2 minutes, 100 ℃ of temperature; Add again 16 parts of agar, after heating and dissolving completely to agar for 2 minutes, add water constant volume, minute install in test tube;
Sterilizing, 120 ℃ of temperature, pressure 101.33 KPa, 20 minutes time obtained bolete mother culture media.
embodiment 8
By 22 parts of glucose that weigh up, 7.5 parts of yeast powders, 3.5 parts of analysis for soybean powder, 0.53 part of KH
2pO
4, 0.6 part of MgSO
4h
2o, puts into container, adds 300 parts of water, places standby; The 18-21 part vegetable mould weighing up is put into beaker and add 250 parts of water boil lixiviates 22 minutes, filter, filtrate is added in container; The dry and soft pin of 48-52 part is put into beaker and add 200 parts of water boil lixiviates 20 minutes, get vat liquor and add in container;
Above material is in vessel in heating 3 minutes, 100 ℃ of temperature; Add again 21 parts of agar, after heating and dissolving completely to agar for 3 minutes, add water constant volume, minute install in test tube;
Sterilizing, 120 ℃ of temperature, pressure 101.33 KPa, 30 minutes time obtained bolete mother culture media.
embodiment 9(control group 1)
Traditional substratum MMN substratum of take is control group 1
0.25 gram of (NH of the amount of weighing up
4)
2hPO
4, 0.5 gram of KH
2pO
4, 0.15 gram of MgSO
47H
2o, 10 grams of glucose, 3 grams of maltose, 55 milligrams of CaCl
22H
2o, 0.25 milligram of NaCl, 20 milligrams of FeCl
3, 100 microgram VitB1s, 25 microgram vitamin Hs are put into container, add 500 ml waters and boil 1-2 minute, add 20 grams of agar to dissolving, water constant volume to 1000 milliliter, autoclave sterilization, 120 ℃ of temperature, pressure 101.33 KPa, obtain bolete mother culture media for 30 minutes.
embodiment 10(control group 2)
By 200 grams of the potatos weighing up, 20 grams of glucose, 2 grams of yeast leach liquors, 1 gram of KH
2pO
4, 40 mcg/ml vitamins Bs
1, put into 1000 ml vol cups, add 300 ml waters, place standby; Above material boils 30 minutes together, after all material dissolves, add 20 grams of agar, after being heated to agar and dissolving completely, with remaining water, be settled to 1000 milliliters, minute install in test tube, conventional autoclave sterilization, 120 ℃ of temperature, pressure 101.33 KPa, obtain bolete mother culture media for 30 minutes.
embodiment 11(control group 3)
By 20 grams of the glucose weighing up, 7 grams of yeast powders, 3 grams of analysis for soybean powder, 1 gram of KH
2pO
4, 0.5 gram of MgSO
4h
2o, 0.5 gram of MnSO
4h
2o, puts into 1000 ml vol cups, adds 300 ml waters, places standby; Above material boils 1-2 minute together, after all medicines dissolve, add 20 grams of agar, after being heated to agar and dissolving completely, with remaining water, be settled to 1000 milliliters, minute install in test tube, conventional autoclave sterilization, 120 ℃ of temperature, pressure 101.33 KPa, obtain bolete mother culture media for 30 minutes.
Cultural method:
Bolete is implanted in mother culture media, and static cultivation in 25 degree incubators, as Boletus bicolor, King Boletus or grey brown bolete etc.
Performance index, as following table: (embodiment 9-11 is control group)
The length of above slant medium is 8 centimetres, speed record be how many millimeters of every days of mycelial growth: millimeter/day (mm/d), can bring back to life to start to grow into from mycelia and cover with the mean value that inclined-plane calculates growth every day.As can be known from the above table, no matter the culture medium culturing bolete in the present invention, be the mycelial speed of growth, cover with test tube slant, or the resurrection time is upper, all fast short with the time than control group.
Claims (5)
1. a bolete mother culture media, metering by weight includes following component:
Glucose 18-24 part
Yeast powder 5-8 part
Analysis for soybean powder 2-4 part
KH
2pO
40.2-0.6 part
MgSO
4h
2o 0.25-0.65 part
Vegetable mould 15-23 part
Dry and soft pin 40-55 part
Agar 14-22 part
Water 800-1500 part.
2. bolete mother culture media according to claim 1, is characterized in that: the preferable amount of its each component is:
Glucose 19-22 part
Yeast powder 6-7.5 part
Analysis for soybean powder 2.5-3.5 part
KH
2pO
40.3-0.53 part
MgSO
4h
2o 0.4-0.6 part
Vegetable mould 18-21 part
Dry and soft pin 48-52 part
Agar 16-21 part
Water 900-1300 part.
3. bolete mother culture media according to claim 2, is characterized in that: the optimum amount of its each component is:
20 parts of glucose
6.5 parts of yeast powders
3 parts of analysis for soybean powder
KH
2pO
40.5 part
MgSO
4h
20.45 part of O
20 parts of vegetable mould
50 parts, dry and soft pin
20 parts, agar
1000 parts, water.
4. the method for preparation bolete mother culture media claimed in claim 1, according to the following steps operation:
Step 1, raw material weighing, cleaning vessel equipment;
Step 2, by load weighted glucose 18-24 part, yeast powder 5-8 part, analysis for soybean powder 2-4 part,
KH
2pO
40.2-0.6 part, MgSO
4h
2o 0.25-0.65 part, puts into container and adds 200-300 part water, places standby;
Step 3, load weighted vegetable mould 15-23 part is put into beaker, add 200-250 part water, boil lixiviate and filter after 20 minutes, filtrate is added in container;
Step 4, dry and soft pin 40-55 part is put into beaker, add 200-250 part water, boil lixiviate 15 minutes, get vat liquor and join in same container;
Step 5, by the mixed solution heating 1-3 minute in said vesse, make temperature reach 90-100 ℃; Add again 14-22 part agar, heat and dissolve completely to agar for 2-3 minute, add water constant volume, minute install in test tube;
Step 6, by the material in test tube under 120 ℃ of temperature, pressure 101.33 KPa, sterilizing 20-30 minute, obtains bolete mother culture media.
5. according to the bolete mother culture media one of claim 1-3 Suo Shu, it is characterized in that: glucose used is medical glucose.
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|---|---|---|---|
| CN201210557661.5A CN103011935B (en) | 2012-12-20 | 2012-12-20 | Boletus mother culture medium and preparation method thereof |
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|---|---|---|---|
| CN201210557661.5A CN103011935B (en) | 2012-12-20 | 2012-12-20 | Boletus mother culture medium and preparation method thereof |
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| CN103011935B true CN103011935B (en) | 2014-03-26 |
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| CN103766137B (en) * | 2013-11-07 | 2016-05-04 | 石泉 | A kind of Boletus aereus cultural method |
| CN103766138B (en) * | 2013-11-07 | 2015-06-17 | 石泉 | Phlebopus portentosus mother culture preserving method |
| CN106673806A (en) * | 2016-12-08 | 2017-05-17 | 河池市农业科学研究所 | Special solid culture medium for boletus edulis and preparation method of special solid culture medium |
| CN106588144A (en) * | 2016-12-16 | 2017-04-26 | 河南农业职业学院 | Edible mushroom mother culture medium formulation and preparation method |
| CN108949578A (en) * | 2017-05-17 | 2018-12-07 | 张胜友 | A kind of bolete liquid spawn culture medium and preparation method thereof |
| CN107162753B (en) * | 2017-06-14 | 2020-08-11 | 东莞东阳光保健品研发有限公司 | Culture medium and method for phlebopus portentosus |
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| CN101836597A (en) * | 2009-03-18 | 2010-09-22 | 西南科技大学 | New grey white variant strain with red pleurotus |
| CN101664150A (en) * | 2009-08-24 | 2010-03-10 | 天津春发食品配料有限公司 | Boletus fermenting liquid composition for preparing essence |
| CN101665379A (en) * | 2009-08-24 | 2010-03-10 | 天津春发食品配料有限公司 | Culture medium for fermenting boletus liquid |
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