CN108934748A - A kind of Rhizoma Gastrodiae plantation symbiosis halimasch production kind culture medium and cultural method with excellent production performance - Google Patents
A kind of Rhizoma Gastrodiae plantation symbiosis halimasch production kind culture medium and cultural method with excellent production performance Download PDFInfo
- Publication number
- CN108934748A CN108934748A CN201810651124.4A CN201810651124A CN108934748A CN 108934748 A CN108934748 A CN 108934748A CN 201810651124 A CN201810651124 A CN 201810651124A CN 108934748 A CN108934748 A CN 108934748A
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- China
- Prior art keywords
- halimasch
- culture medium
- symbiosis
- rhizoma gastrodiae
- production performance
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
Rhizoma Gastrodiae plantation symbiosis provided by the invention with excellent production performance produces kind of a culture medium with halimasch, including bamboo pole, solid culture medium and water, the solid culture medium is made of corn flour 55~85, sawdust 20~30, wheat bran 10~25,2~5 parts of land plaster, 1~5 part of white sugar, 1~5 part of agar powder, 1~3 part of potassium dihydrogen phosphate, 1~3 part of magnesium sulfate, 0.1~0.3 part of acetic acid.It utilizes the Rhizoma Gastrodiae plantation symbiosis method that halimasch produces kind of culture medium culture halimasch of the present invention with excellent production performance: (1) acquiring Wild gastrodia and grow the halimasch shoestring in preferable regional soil；(2) prepare bamboo pole；(3) semisolid culturemedium is prepared；(4) it packs；(5) it is inoculated with；(6) it cultivates.The method of the present invention, the halimasch mycelia turned out is sturdy, and mutual adhesion is less, and separation is easy not hurt mycelia.
Description
Technical field
The invention belongs to Rhizoma Gastrodiae planting technology field, in particular to Rhizoma Gastrodiae plants the symbiosis culture of halimasch.
Background technique
Rhizoma Gastrodiae (Gastrodiaelata) is China's tradition rare traditional Chinese medicine, is orchid family Orchidaceae perennial herb
Plant, rhizomes is plump, no greenery, cannot absorb the nutrition in soil by root as general green plants, can not lead to
It crosses photosynthesis and synthesizes substance required for itself, in its growth course, it is necessary to pass through a kind of fungi-halimasch
(Armillariamellea) nutrition is provided for it.Armillaria in Basidiomycetes, Agaricales, fungi a kind of facultative parasitism
Fungi is often born on dead wood or timber, is known as stronger decomposing force to lignin and fiber, mycelia and shoestring can be with Rhizoma Gastrodiae blocks
Stem combines, and provides nutrition required for growth for Rhizoma Gastrodiae.The halimasch pure culture that can be directly used for bacterium material culture is known as producing kind
Or cultivar.During gastrodin cultivation, halimasch production kind is directly used in the culture of bacteria stick, bacterium branch.
It is produced for Rhizoma Gastrodiae and obtains high yield, fine quality production effect, it is crucial institute that cultivating, which has the halimasch of excellent production performance,
?.
Currently, the culture of halimasch production kind is generally primary raw material by bottling, sterilizing, connect using the short tree rod of small weedtree
Kind and by the culture of certain time, after mycelia covers with bottle as production kind use.But it is existing that weedtree stick is used to consolidate to be main
Mycelia is very thin after halimasch that body culture medium raw material obtains is mature and stick to each other is tangled, and is not readily separated, and when separation easily hurts mycelia,
Cause success rate of vaccination during Rhizoma Gastrodiae is planted not high, or timely, enough can not provide growth required nutrition for Rhizoma Gastrodiae, makes
It is smaller or downright bad at Rhizoma Gastrodiae component when harvest.
Goal of the invention
It is an object of that present invention to provide a kind of, and the Rhizoma Gastrodiae plantation symbiosis with excellent production performance produces kind of a culture medium with halimasch
And cultural method, the halimasch mycelia turned out is sturdy, and mutual adhesion is less, and separation is easy not hurt mycelia.
Rhizoma Gastrodiae plantation symbiosis provided by the invention with excellent production performance produces kind of a culture medium, including bamboo with halimasch
Rod, solid culture medium and water, the solid culture medium is by corn flour 55~85, sawdust 20~30, wheat bran 10~25, land plaster 2
~5 parts, 1~5 part of white sugar, 1~5 part of agar powder, 1~3 part of potassium dihydrogen phosphate, 0.1~0.3 part of 1~3 part of magnesium sulfate, acetic acid group
At.
Further, the bamboo pole is length 10-20cm, the fresh bamboo branch item of 3~5cm of diameter.
Further, the weight ratio of the solid culture medium and water is 1:(2~3).
Kind of a culture medium culture is produced with halimasch using the Rhizoma Gastrodiae plantation symbiosis of the present invention with excellent production performance
The method of halimasch, comprising the following steps:
(1) acquire: acquisition Wild gastrodia grows the halimasch shoestring in preferable regional soil, carefully cleans silt, and with sterile
Water rinses 2~3 times, impregnates 30s in 75% alcoholic solution, with aseptic water washing 4~5 times, 40s is rinsed every time, with sterile filter
Paper blots its surface moisture；
(2) prepare bamboo pole: choosing fresh bamboo branch of uniform size, the bamboo pole of equal length is whittled into knife, clear water, which is cleaned, to be used in combination
30s disinfection is impregnated in 75% alcoholic solution；
(3) semisolid culturemedium is prepared: by corn flour 55~85, sawdust 20~30, wheat bran 10~25,2~5 parts of land plaster, white
1~5 part of sugar, 1~5 part of agar powder, 1~3 part of potassium dihydrogen phosphate, 1~3 part of magnesium sulfate, 0.1~0.3 part of acetic acid composition, it is mixed with water
Conjunction is mixed thoroughly in semi-solid；
(4) it packs: semisolid culturemedium being contained in polyethylene bag body, 4~8 bamboo poles are uniformly inserted into semisolid culturemedium
In, the binding and fixing hard annulus at sack forms the sack of fixed shape, tight mouth vial-type compost is integrally formed after installing
Bag；
(5) it is inoculated with: the halimasch shoestring that step (1) obtains is seeded at culture material bag sack, one section of long 1cm of every bottle of inoculation~
The shoestring of 2cm or so closes covering sack with waste paper；
(6) it cultivates: the material bag after inoculation being transferred to the culturing room being protected from light and is cultivated under the conditions of 22~25 DEG C of temperature to halimasch
After shoestring covers with material bag.
Further, in step (3), the weight ratio of the solid culture medium and water is 1: (2~3).
Further, bamboo pole length is 10~20cm in step (2), and bamboo pole diameter is 3~5cm.
Compared with prior art, the invention has the following advantages:
1, after the method for the invention replaces weedtree rod, halimasch mature using bamboo pole, growth rhythm is uniform, and halimasch mycelia is thick
Strong and mutual adhesion is less, and separation is easy and does not hurt mycelia, is conducive to provide to kind fiber crops rapidly during Rhizoma Gastrodiae is planted
Nutrition, give full measure after Rhizoma Gastrodiae is mature, head it is big.
3, the method for the invention replaces weedtree rod using bamboo pole, and after using weedtree rod, halimasch mature, ring is hard
Degree is still higher, can utilize, economize on resources again.
4, in culture medium of the present invention, with corn flour for main compost, cooperate soybean powder and microelement, can be honey
Nutrition needed for the growth of ring bacterium provides.
Specific embodiment
Rhizoma Gastrodiae plantation symbiosis to of the present invention with excellent production performance below with halimasch produce kind of culture medium and
Cultural method is described further.
Embodiment 1
Culture medium prescription: by length 10cm, the bamboo pole of diameter 5cm, solid culture medium and water composition, the solid culture medium is by jade
Rice flour 55, sawdust 30,12 parts of wheat bran, 5 parts of land plaster, 1 part of white sugar, agar powder 5 divide, 1 part of potassium dihydrogen phosphate, 3 parts of magnesium sulfate, vinegar
0.1 part of composition of acid.The weight ratio of the solid culture medium and water is 1: 2.
(1) acquire: acquisition Wild gastrodia grows the halimasch shoestring in preferable regional soil, carefully cleans silt, is used in combination
Aseptic water washing 2~3 times, 30s is impregnated in 75% alcoholic solution, with aseptic water washing 4~5 times, 40s is rinsed every time, with nothing
Bacterium filter paper blots its surface moisture；
(2) prepare bamboo pole: choosing fresh bamboo branch of uniform size, be whittled into length with knife and want the bamboo pole waited, clear water, which is cleaned, to be used in combination
30s disinfection is impregnated in 75% alcoholic solution；
(3) it prepares semisolid culturemedium: the solid culture medium of formula ratio being mixed with water and is mixed thoroughly in semi-solid；
(4) it packs: semisolid culturemedium being contained in polyethylene bag body, 4 bamboo poles are uniformly inserted into semisolid culturemedium,
Binding and fixing hard annulus at sack forms the sack of fixed shape, tight mouth vial-type culture material bag is integrally formed after installing；
(5) it is inoculated with: the halimasch shoestring that step (1) obtains is seeded at culture material bag sack, every bottle of inoculation 1cm one section long is left
Right shoestring closes covering sack with waste paper；
(6) it cultivates: the material bag after inoculation being transferred to the culturing room being protected from light and is cultivated under the conditions of 22 DEG C of temperature to halimasch shoestring
After covering with material bag.
Embodiment 2
Culture medium prescription: by length 15cm, the bamboo pole of diameter 4cm, solid culture medium and water composition, the solid culture medium is by jade
Rice flour 85, sawdust 20,25 parts of wheat bran, 2 parts of land plaster, 5 parts of white sugar, 1 part of agar powder, 3 parts of potassium dihydrogen phosphate, 1 part of magnesium sulfate, vinegar
0.3 part of composition of acid.The weight ratio of the solid culture medium and water is 1: 3.
(1) acquire: acquisition Wild gastrodia grows the halimasch shoestring in preferable regional soil, carefully cleans silt, is used in combination
Aseptic water washing 2~3 times, 30s is impregnated in 75% alcoholic solution, with aseptic water washing 4~5 times, 40s is rinsed every time, with nothing
Bacterium filter paper blots its surface moisture；
(2) prepare bamboo pole: choosing fresh bamboo branch of uniform size, be whittled into length with knife and want the bamboo pole waited, clear water, which is cleaned, to be used in combination
30s disinfection is impregnated in 75% alcoholic solution；
(3) it prepares semisolid culturemedium: the solid culture medium of formula ratio being mixed with water and is mixed thoroughly in semi-solid；
(4) it packs: semisolid culturemedium being contained in polyethylene bag body, 4 bamboo poles are uniformly inserted into semisolid culturemedium,
Binding and fixing hard annulus at sack forms the sack of fixed shape, tight mouth vial-type culture material bag is integrally formed after installing；
(5) it is inoculated with: the halimasch shoestring that step (1) obtains is seeded at culture material bag sack, every bottle of inoculation 1cm one section long is left
Right shoestring closes covering sack with waste paper；
(6) it cultivates: the material bag after inoculation being transferred to the culturing room being protected from light and is cultivated under the conditions of 22 DEG C of temperature to halimasch shoestring
After covering with material bag.
Embodiment 3
Culture medium prescription: by length 10cm, the bamboo pole of diameter 5cm, solid culture medium and water composition, the solid culture medium is by jade
Rice flour 60, sawdust 27,18 parts of wheat bran, 3 parts of land plaster, 4 parts of white sugar, 3 parts of agar powder, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, vinegar
0.3 part of composition of acid.The weight ratio of the solid culture medium and water is 1: 3.
(1) acquire: acquisition Wild gastrodia grows the halimasch shoestring in preferable regional soil, carefully cleans silt, is used in combination
Aseptic water washing 2~3 times, 30s is impregnated in 75% alcoholic solution, with aseptic water washing 4~5 times, 40s is rinsed every time, with nothing
Bacterium filter paper blots its surface moisture；
(2) prepare bamboo pole: choosing fresh bamboo branch of uniform size, be whittled into length with knife and want the bamboo pole waited, clear water, which is cleaned, to be used in combination
30s disinfection is impregnated in 75% alcoholic solution；
(3) it prepares semisolid culturemedium: the solid culture medium of formula ratio being mixed with water and is mixed thoroughly in semi-solid；
(4) it packs: semisolid culturemedium being contained in polyethylene bag body, 4 bamboo poles are uniformly inserted into semisolid culturemedium,
Binding and fixing hard annulus at sack forms the sack of fixed shape, tight mouth vial-type culture material bag is integrally formed after installing；
(5) it is inoculated with: the halimasch shoestring that step (1) obtains is seeded at culture material bag sack, every bottle of inoculation 1cm one section long is left
Right shoestring closes covering sack with waste paper；
(6) it cultivates: the material bag after inoculation being transferred to the culturing room being protected from light and is cultivated under the conditions of 22 DEG C of temperature to halimasch shoestring
After covering with material bag.
Claims (6)
1. a kind of Rhizoma Gastrodiae plantation symbiosis with excellent production performance produces kind of a culture medium, including the training of bamboo pole, solid with halimasch
Nutriment and water, the solid culture medium is by corn flour 55~85, sawdust 20~30, wheat bran 10~25,2~5 parts of land plaster, white sugar
1~5 part, 1~5 part of agar powder, 1~3 part of potassium dihydrogen phosphate, 1~3 part of magnesium sulfate, 0.1~0.3 part of acetic acid composition.
2. the Rhizoma Gastrodiae plantation symbiosis with excellent production performance produces kind of a culture medium with halimasch according to claim 1,
It is characterized in that, the bamboo pole is 10~20cm of length, the fresh bamboo branch item of 3~5cm of diameter.
3. the Rhizoma Gastrodiae plantation symbiosis with excellent production performance produces kind of a culture medium with halimasch according to claim 1,
It is characterized in that, the weight ratio of the solid culture medium and water is 1:(2~3).
4. producing kind of a culture medium culture with halimasch using the Rhizoma Gastrodiae plantation symbiosis described in claim 1 with excellent production performance
The method of halimasch, comprising the following steps:
(1) acquire: acquisition Wild gastrodia grows the halimasch shoestring in preferable regional soil, carefully cleans silt, and with sterile
Water rinses 2~3 times, impregnates 30s in 75% alcoholic solution, with aseptic water washing 4~5 times, 40s is rinsed every time, with sterile filter
Paper blots its surface moisture；
(2) prepare bamboo pole: choosing fresh bamboo branch of uniform size, be whittled into length with knife and want the bamboo pole waited, clear water, which is cleaned, to be used in combination
30s disinfection is impregnated in 75% alcoholic solution；
(3) prepare semisolid culturemedium: by corn flour special 55~85, rice bran 20~30,5~10 parts of soybean powder, land plaster 2~
5 parts, 1~5 part of glucose, 1~5 part of agar powder, 1~3 part of potassium dihydrogen phosphate, 1~3 part of magnesium sulfate, 0.1~0.3 part of acetic acid, with
Water mixing is mixed thoroughly in semi-solid；
(4) it packs: semisolid culturemedium being contained in polyethylene bag body, 4~8 bamboo poles are uniformly inserted into semisolid culturemedium
In, the binding and fixing hard annulus at sack forms the sack of fixed shape, tight mouth vial-type compost is integrally formed after installing
Bag；
(5) it is inoculated with: the halimasch shoestring that step (1) obtains is seeded at culture material bag sack, one section of long 1cm of every bottle of inoculation~
The shoestring of 2cm or so closes covering sack with waste paper；
(6) it cultivates: the material bag after inoculation being transferred to the culturing room being protected from light and is cultivated under the conditions of 22~25 DEG C of temperature to halimasch
After shoestring covers with material bag.
5. producing kind of a culture medium with halimasch using the Rhizoma Gastrodiae plantation symbiosis with excellent production performance according to claim 4
The method for cultivating halimasch, which is characterized in that the weight ratio of the solid culture medium and water is 1: (2~3).
6. producing kind of a culture medium with halimasch using the Rhizoma Gastrodiae plantation symbiosis with excellent production performance according to claim 4
The method for cultivating halimasch, which is characterized in that bamboo pole length is 10~20cm in step (2), and bamboo pole diameter is 3~5cm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114303786A (en) * | 2021-12-09 | 2022-04-12 | 陕西春森菌业有限公司 | Preparation method of semi-solid armillaria mellea strain |
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2018
- 2018-06-22 CN CN201810651124.4A patent/CN108934748A/en active Pending
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| CN114303786A (en) * | 2021-12-09 | 2022-04-12 | 陕西春森菌业有限公司 | Preparation method of semi-solid armillaria mellea strain |
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Application publication date: 20181207 |