CN108925982A - A kind of preparation method of high anti-oxidation activity pomegranate ferment - Google Patents
A kind of preparation method of high anti-oxidation activity pomegranate ferment Download PDFInfo
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- 235000014360 Punica granatum Nutrition 0.000 title claims abstract description 55
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 230000000694 effects Effects 0.000 title claims abstract description 20
- 230000003064 anti-oxidating effect Effects 0.000 title claims abstract description 19
- 241000219991 Lythraceae Species 0.000 title claims abstract 12
- 238000000855 fermentation Methods 0.000 claims abstract description 35
- 230000004151 fermentation Effects 0.000 claims abstract description 35
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 21
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 21
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 19
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000011218 seed culture Methods 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 19
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 14
- 239000002994 raw material Substances 0.000 claims description 13
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 11
- 239000012153 distilled water Substances 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 235000015278 beef Nutrition 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 6
- 229920000053 polysorbate 80 Polymers 0.000 claims description 6
- 239000001632 sodium acetate Substances 0.000 claims description 6
- 235000017281 sodium acetate Nutrition 0.000 claims description 6
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 6
- 239000001393 triammonium citrate Substances 0.000 claims description 6
- 235000011046 triammonium citrate Nutrition 0.000 claims description 6
- 210000002615 epidermis Anatomy 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 2
- 238000011068 loading method Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims 1
- 241000186660 Lactobacillus Species 0.000 claims 1
- 229940039696 lactobacillus Drugs 0.000 claims 1
- 230000003078 antioxidant effect Effects 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 5
- 244000294611 Punica granatum Species 0.000 description 43
- 229930014626 natural product Natural products 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 7
- 230000002000 scavenging effect Effects 0.000 description 7
- 244000269722 Thea sinensis Species 0.000 description 6
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 description 4
- 235000013824 polyphenols Nutrition 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 description 3
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 3
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 3
- 150000001450 anions Chemical class 0.000 description 3
- 239000003205 fragrance Substances 0.000 description 3
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000008204 material by function Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of preparation methods of high anti-oxidation activity pomegranate ferment, it is that juice of my pomegranate, pure water, brown sugar and catechol are packed into ferment bottle, 5~8 hours are stood, then addition lactobacillus plantarum fermentation liquid, up to high anti-oxidation activity pomegranate ferment after standing for fermentation.The method of the present invention can significantly improve ferment antioxidant activity, have preferable health-care efficacy.
Description
Technical field
The invention belongs to field of fermentation engineering, and in particular to a kind of preparation method of high anti-oxidation activity pomegranate ferment.
Technical background
Pomegranate is a kind of one of very important fruit in China.There is cultivation in China north and south, with Anhui, Jiangsu, Henan etc.
Ground cultivated area is larger, and cultivates some more good kinds.The nutrition of pomegranate is very rich, containing there are many needed by human body
Nutritional ingredient contains vitamin C and B family vitamin, organic acid, carbohydrate, protein, fat and calcium, phosphorus, potassium etc. in fruit
Minerals.According to analysis, carbohydrate containing 17% in pomegranate fruit, moisture content 79%, sugar 13~17%, one of main composition
It is Punica granatum L. polyphenol, is main oxidation-resistant active ingredient.
Enzyme food is with one or more fresh water fruits and vegetables, mushroom, Chinese herbal medicine etc. for raw material, through multiple-microorganism
Made of fermentation, the functional hair of the ingredients such as small molecule functional materials, polysaccharide and other secondary metabolites rich in
Ferment product.The manufacture craft of ferment is varied at present, and fermentation time lacks then some months, more then several years.It is with anti-oxidant, increasing
The effect of strong immune, promotion digestion, rescue protect liver etc., pursuing high health-care efficacy is requirement of the people to product.
Summary of the invention
It is an object of the present invention to provide a kind of preparation methods of high anti-oxidation activity pomegranate ferment.The method of the present invention can be significant
Ferment antioxidant activity is improved, there is preferable health-care efficacy.
The preparation method of high anti-oxidation activity pomegranate ferment of the present invention, includes the following steps:
Step 1: taking the pomegranate of juice of my pomegranate total phenol content >=0.18% as raw material, pomegranate epidermis is cleaned, then smelly
Overflow-type submergence is carried out in oxygen water sterilization 5~15 minutes, successively remove the peel after draining, beat juice, obtain juice of my pomegranate；
Step 2: the preparation of lactobacillus plantarum fermentation liquid
2a, slant strains culture: two inoculation circular rector lactobacillus plantarums are incubated in MRS slant medium, 30~37
It is cultivated 3~5 days at DEG C；
The preparation of 2b, liquid spawn: slant strains are directly forwarded to MRS seed culture medium (every 50ml seed culture medium
It is inoculated with an oese) in, stationary culture 2~4 days, are prepared seed liquor at 30~37 DEG C；
2c, expand culture: by gained seed liquor by volume 10% inoculum concentration be forwarded in MRS seed culture medium, 30
Stationary culture 1-3 days at~37 DEG C, obtains lactobacillus plantarum fermentation liquid；
The formula of the MRS slant medium constitutes as follows:
Peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate
2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, agar powder 15.0g, distilled water
1000mL.It is dissolved by heating after above-mentioned raw materials are measured mixing according to the ratio, corrects pH value 6.2, then the high pressure sterilization 15- at 121 DEG C
20min.
The formula of the MRS seed culture medium constitutes as follows:
Peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate
2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, agar powder 15.0g, distilled water
1000mL.It is dissolved by heating after above-mentioned raw materials are measured mixing according to the ratio, corrects pH value 6.2, then the high pressure sterilization 15- at 121 DEG C
20min.
Step 3: fermentation
By mass fraction by 50~80 parts of juice of my pomegranate, 20~40 parts of pure water, 20~50 parts of brown sugar and purity 80 ± 3%
0.1~0.2 part of loading ferment bottle of catechol stands 5~8 hours, adds lactobacillus plantarum fermentation liquid by the mass ratio of 10:1,
It is left to ferment 10~15 days at 30~37 DEG C, up to high anti-oxidation activity pomegranate ferment after filtering.
The beneficial effects of the present invention are embodied in:
1, raw material of the present invention is mainly pomegranate, and it is relatively inexpensive that pomegranate is only used as fruit to eat price, develop for ferment have compared with
High added value.
2, the method for the present invention can significantly improve ferment antioxidant activity, have preferable health-care efficacy.
3, present invention process is simple, and fermentation time is short, at low cost, has preferable economic value.
Specific embodiment
The present invention measures oxidation resistance according to the following method
(a) measurement of DPPH Scavenging activity:
Enzyme liquid accurately is weighed, is sufficiently dissolved with appropriate distilled water, the diluent A that concentration is 1g/100ml is made.
Each 2ml of accurate measuring dilution and 2ml concentration are 2.0 × 10-4The DPPH solution of mol/L mixes, and places after shaking up
30 minutes, be control with corresponding solvent, measuring above-mentioned solution in wavelength with ultraviolet-uisible spectrophotometer is at 517nm
Absorbance (Ai), obtain AiValue.Accurate measuring dilution 2ml solvent corresponding with 2ml is control with solvent after mixing,
Measuring above-mentioned solution in wavelength with ultraviolet-uisible spectrophotometer is the absorbance (A at 517nmj), obtain AjValue.Accurate measuring
2ml concentration is 2.0 × 10-4The DPPH solution of mol/L is mixed with distilled water, is control with corresponding solvent, is used UV, visible light
The above-mentioned solution of spectrophotometric determination is in the absorbance (A that wavelength is at 517nmc).DPPH Scavenging activity calculation formula are as follows: [1-
(Ai-Aj)/Ac]×100
(b) measurement of superoxide anion Scavenging activity:
Enzyme liquid accurately is weighed, is sufficiently dissolved with appropriate distilled water, the diluent A that concentration is 1g/100ml is made.
Trishydroxymethylaminomethane-hydrochloric acid buffer solution (pH8.2) 4.5ml for taking 0.05mol/L, sets in 25 DEG C of water-baths
After twenty minutes, 1ml dilute solution and 0.4ml 25mmol/L pyrogallol solution is added in preheating, anti-in 25 DEG C of water-baths after mixing
It answers 1 minute, 8mmol/L HCl 1ml is added and terminates reaction, measures the suction of solution under ultraviolet-visible spectrophotometer at 299nm
Luminosity (Ai), obtain AiValue.Blank control group replaces sample solution with the distilled water of same volume, in UV, visible light spectrophotometric
Count the absorbance (A that solution is measured at lower 299nm0), respectively obtain A0Value.Superoxide anion Scavenging activity calculation formula are as follows: (A0-
Ai)/A0×100。
The present invention is described in detail below in conjunction with specific embodiment.
The formula of MRS slant medium used in the embodiment of the present invention constitutes as follows:
Peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate
2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, agar powder 15.0g, distilled water
1000mL.It is dissolved by heating after above-mentioned raw materials are measured mixing according to the ratio, corrects pH value 6.2, then the high pressure sterilization 15- at 121 DEG C
20min.
The formula of MRS seed culture medium used in the embodiment of the present invention constitutes as follows:
Peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate
2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, agar powder 15.0g, distilled water
1000mL.It is dissolved by heating after above-mentioned raw materials are measured mixing according to the ratio, corrects pH value 6.2, then the high pressure sterilization 15- at 121 DEG C
20min.
Embodiment 1:
In the present embodiment high anti-oxidation activity pomegranate ferment the preparation method is as follows:
1, it takes the pomegranate of juice of my pomegranate total phenol content 0.18% as raw material, pomegranate epidermis is cleaned, then in Ozone Water
It carries out overflow-type submergence sterilization 5 minutes, is successively removed the peel after draining, beats juice, obtain juice of my pomegranate；
2, the preparation of lactobacillus plantarum fermentation liquid
2a, slant strains culture: the lactobacillus plantarum strain two for being purchased from Shaanxi Sen Fu natural product Co., Ltd is inoculated with
Circular rector accesses in MRS slant medium, cultivates 3 days at 30 DEG C；
The preparation of 2b, liquid spawn: slant strains are directly forwarded to MRS seed culture medium (every 50ml seed culture medium
It is inoculated with an oese) in, stationary culture 2 days, are prepared seed liquor at 30 DEG C；
2c, expand culture: by gained seed liquor by volume 10% inoculum concentration be forwarded in MRS seed culture medium, 30
Stationary culture 1 day at DEG C, obtains lactobacillus plantarum fermentation liquid；
3, it ferments
By mass fraction by 0.2 part of catechol of 50 parts of juice of my pomegranate, 20 parts of pure water, 20 parts of brown sugar and purity 80 ± 3%
It is packed into ferment bottle, stands 5 hours, lactobacillus plantarum fermentation liquid is added by the mass ratio of 10:1, is left to ferment 10 days at 30 DEG C,
Up to high anti-oxidation activity pomegranate ferment after filtering, taste is sour-sweet, while having light fragrance.
The non-fermentation medium fully according to the preparation of 1 scheme of embodiment, the ferment to ferment completely by embodiment 1 are measured respectively
(juice of my pomegranate amount containing total phenol 0.14%), the ferment (being not added with tea phenol) to ferment completely by embodiment 1, with 1 scheme of embodiment (hair
Yeast-like fungi kind be lactobacillus acidophilus, be purchased from Shaanxi Sen Fu natural product Co., Ltd) preparation ferment, with 1 scheme of embodiment (fermentation
Strain is Lactobacillus rhamnosus, is purchased from Shaanxi Sen Fu natural product Co., Ltd) DPPH of the ferment of preparation removes vigor and super
Clear anion Scavenging activity (being shown in Table 1), as a result, it has been found that the ferment that juice of my pomegranate total phenol content improves after improving fermentation to a certain degree is anti-
Oxidation activity, addition tea polyphenols can significantly improve antioxidant activity, and preferably strain can improve the ferment after fermentation to a certain degree
Antioxidant activity.
1 different disposal of table is compared with the oxidation resistance of this programme
Embodiment 2:
In the present embodiment high anti-oxidation activity pomegranate ferment the preparation method is as follows:
1, it takes the pomegranate of juice of my pomegranate total phenol content 0.21% as raw material, pomegranate epidermis is cleaned, then in Ozone Water
It carries out overflow-type submergence sterilization 15 minutes, is successively removed the peel after draining, beats juice, obtain juice of my pomegranate；
2, the preparation of lactobacillus plantarum fermentation liquid
2a, slant strains culture: the lactobacillus plantarum strain for being purchased from Shaanxi Sen Fu natural product Co., Ltd is accessed into MRS
In slant medium, cultivated 5 days at 37 DEG C；
The preparation of 2b, liquid spawn: slant strains are directly forwarded to MRS seed culture medium (every 50ml seed culture medium
It is inoculated with an oese) in, stationary culture 4 days, are prepared seed liquor at 37 DEG C；
2c, expand culture: by gained seed liquor by volume 10% inoculum concentration be forwarded in MRS seed culture medium, 37
Stationary culture 3 days at DEG C, obtain lactobacillus plantarum fermentation liquid；
3, it ferments
By mass fraction by 0.2 part of catechol of 80 parts of juice of my pomegranate, 40 parts of pure water, 50 parts of brown sugar and purity 80 ± 3%
It is packed into ferment bottle, stands 8 hours, lactobacillus plantarum fermentation liquid is added by the mass ratio of 10:1, is left to ferment 15 days at 37 DEG C,
Up to high anti-oxidation activity pomegranate ferment after filtering, taste is sour-sweet, while having light fragrance.
The non-fermentation medium fully according to the preparation of 2 scheme of embodiment, the ferment to ferment completely by embodiment 2 are measured respectively
(juice of my pomegranate amount containing total phenol 0.14%), the ferment (being not added with tea phenol) to ferment completely by embodiment 2, with 2 scheme of embodiment (hair
Yeast-like fungi kind be lactobacillus acidophilus, be purchased from Shaanxi Sen Fu natural product Co., Ltd) preparation ferment, with 2 scheme of embodiment (fermentation
Strain is Lactobacillus rhamnosus, is purchased from Shaanxi Sen Fu natural product Co., Ltd) DPPH of the ferment of preparation removes vigor and super
Clear anion Scavenging activity (being shown in Table 2), as a result, it has been found that the ferment that juice of my pomegranate total phenol content improves after improving fermentation to a certain degree is anti-
Oxidation activity, addition tea polyphenols can significantly improve antioxidant activity, and preferably strain can improve the ferment after fermentation to a certain degree
Antioxidant activity.
2 different disposal of table is compared with this programme oxidation resistance
Embodiment 3:
In the present embodiment high anti-oxidation activity pomegranate ferment the preparation method is as follows:
1, it takes the pomegranate of juice of my pomegranate total phenol content 0.21% as raw material, pomegranate epidermis is cleaned, then in Ozone Water
It carries out overflow-type submergence sterilization 10 minutes, is successively removed the peel after draining, beats juice, obtain juice of my pomegranate；
2, the preparation of lactobacillus plantarum fermentation liquid
2a, slant strains culture: the lactobacillus plantarum strain for being purchased from Shaanxi Sen Fu natural product Co., Ltd is accessed into MRS
In slant medium, cultivated 5 days at 33 DEG C；
The preparation of 2b, liquid spawn: slant strains are directly forwarded to MRS seed culture medium (every 50ml seed culture medium
It is inoculated with an oese) in, stationary culture 4 days, are prepared seed liquor at 33 DEG C；
2c, expand culture: by gained seed liquor by volume 10% inoculum concentration be forwarded in MRS seed culture medium, 33
Stationary culture 3 days at DEG C, obtain lactobacillus plantarum fermentation liquid；
3, it ferments
By mass fraction by 0.1 part of catechol of 60 parts of juice of my pomegranate, 30 parts of pure water, 35 parts of brown sugar and purity 80 ± 3%
It is packed into ferment bottle, stands 8 hours, lactobacillus plantarum fermentation liquid is added by the mass ratio of 10:1, is left to ferment 12 days at 33 DEG C,
Up to high anti-oxidation activity pomegranate ferment after filtering, taste is sour-sweet, while having light fragrance.
The non-fermentation medium fully according to the preparation of 3 scheme of embodiment, the ferment to ferment completely by embodiment 3 are measured respectively
(juice of my pomegranate amount containing total phenol 0.14%), the ferment (being not added with tea phenol) to ferment completely by embodiment 3, with 3 scheme of embodiment (hair
Yeast-like fungi kind be lactobacillus acidophilus, be purchased from Shaanxi Sen Fu natural product Co., Ltd) preparation ferment, with 3 scheme of embodiment (fermentation
Strain is Lactobacillus rhamnosus, is purchased from Shaanxi Sen Fu natural product Co., Ltd) DPPH of the ferment of preparation removes vigor and super
Clear anion Scavenging activity (being shown in Table 3), as a result, it has been found that the ferment that juice of my pomegranate total phenol content improves after improving fermentation to a certain degree is anti-
Oxidation activity, addition tea polyphenols can significantly improve antioxidant activity, and preferably strain can improve the ferment after fermentation to a certain degree
Antioxidant activity.
3 different disposal of table is compared with this programme oxidation resistance
Claims (7)
1. a kind of preparation method of high anti-oxidation activity pomegranate ferment, it is characterised in that include the following steps:
Step 1: taking the pomegranate of juice of my pomegranate total phenol content >=0.18% as raw material, pomegranate epidermis is cleaned, then in Ozone Water
Middle progress overflow-type submergence sterilization 5~15 minutes, successively removes the peel after draining, beats juice, obtain juice of my pomegranate；
Step 2: the preparation of lactobacillus plantarum fermentation liquid
2a, slant strains culture: two inoculation circular rector lactobacillus plantarums are incubated in MRS slant medium, at 30~37 DEG C
Culture 3~5 days；
The preparation of 2b, liquid spawn: slant strains are directly forwarded in MRS seed culture medium, and training is stood at 30~37 DEG C
It supports 2~4 days, seed liquor is prepared；
2c, expand culture: gained seed liquor being forwarded in MRS seed culture medium, stationary culture 1~3 day, obtains at 30~37 DEG C
To lactobacillus plantarum fermentation liquid；
Step 3: fermentation
The catechol of juice of my pomegranate, pure water, brown sugar and purity 80 ± 3% is packed into ferment bottle, stands 5~8 hours, adds plant
Lactobacillus ferment liquid is left to ferment 10~15 days at 30~37 DEG C, up to high anti-oxidation activity pomegranate ferment after filtering.
2. preparation method according to claim 1, it is characterised in that:
The formula of the MRS slant medium constitutes as follows:
Peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate
2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, agar powder 15.0g, distilled water
1000mL.It is dissolved by heating after above-mentioned raw materials are measured mixing according to the ratio, corrects pH value 6.2, then the high pressure sterilization 15- at 121 DEG C
20min.
3. preparation method according to claim 1, it is characterised in that:
The formula of the MRS seed culture medium constitutes as follows:
Peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate
2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, agar powder 15.0g, distilled water
1000mL.It is dissolved by heating after above-mentioned raw materials are measured mixing according to the ratio, corrects pH value 6.2, then the high pressure sterilization 15- at 121 DEG C
20min.
4. preparation method according to claim 1, it is characterised in that:
In step 2b, inoculum concentration when slant strains to be directly forwarded to MRS seed culture medium is every 50ml MRS seed culture
Base is inoculated with an oese.
5. preparation method according to claim 1, it is characterised in that:
In step 2c, seed liquor by volume 10% inoculum concentration be forwarded in MRS seed culture medium.
6. preparation method according to claim 1, it is characterised in that:
In step 3, by mass fraction by 50~80 parts of juice of my pomegranate, 20~40 parts of pure water, 20~50 parts of brown sugar and purity 80 ±
3% 0.1~0.2 part of loading ferment bottle of catechol.
7. preparation method according to claim 1 or 6, it is characterised in that:
Lactobacillus plantarum fermentation liquid is added by the mass ratio of 10:1.
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