CN112190513A

CN112190513A - Pomegranate peel and schisandra chinensis fermentation stock solution as well as preparation method and application thereof

Info

Publication number: CN112190513A
Application number: CN202011236180.5A
Authority: CN
Inventors: 刘慧敏; 许鹏飞; 王伟; 刘清雷; 王肖肖; 雷胜楠; 孙士欣
Original assignee: Shanghai Institute of Technology
Current assignee: Shanghai Institute of Technology
Priority date: 2020-11-09
Filing date: 2020-11-09
Publication date: 2021-01-08
Anticipated expiration: 2040-11-09
Also published as: CN112190513B

Abstract

The invention provides a pomegranate bark and schisandra chinensis fermentation stock solution as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The preparation method of the pomegranate bark and schisandra chinensis fermentation stock solution provided by the invention comprises the following steps: inoculating fermentation bacteria to pericarpium Granati and fructus Schisandrae chinensis, fermenting, centrifuging, and collecting supernatant to obtain pericarpium Granati and fructus Schisandrae chinensis fermentation stock solution; the zymophyte comprises lactobacillus plantarum and streptococcus thermophilus. According to the invention, the lactobacillus plantarum and the streptococcus thermophilus are adopted to carry out whole-plant fermentation on the pomegranate rind and the schisandra chinensis, so that all functional components and the activity of the plants are reserved, the loss of the active components is avoided, the content of the active components is increased, and the prepared fermentation stock solution has a good DPPH free radical removing function and whitening and freckle removing effects; in addition, the preparation method of the invention uses pure natural plant fermentation, does not add any chemical component, belongs to pure natural and green raw materials, is safe and environment-friendly, and meets the requirements of modern cosmetics on functionality and safety.

Description

Pomegranate peel and schisandra chinensis fermentation stock solution as well as preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to pomegranate bark and schisandra chinensis fermentation stock solution as well as a preparation method and application thereof.

Background

With the rapid development of modern biotechnology and the improvement of living standard, people improve the consumption grade of beauty products, natural, green, safe and functional cosmetics without side effect are more and more favored by consumers, and the ferment as a pure natural raw material product just meets the requirements of modern cosmetics. However, the research on the ferment cosmetics in China is still in the beginning stage, the functional effect of the ferment applied to the cosmetics is poor, such as the whitening effect or the anti-aging effect, the anti-oxidation effect and the like, and the development of a ferment product with good functional effect applied to the cosmetic industry is urgently needed at present.

Disclosure of Invention

In order to solve the problems, the invention provides a pomegranate bark and schisandra chinensis fermentation stock solution as well as a preparation method and application thereof. The fermentation stock solution prepared by the preparation method of the pomegranate bark and schisandra chinensis fermentation stock solution provided by the invention contains rich ellagic acid, lignan, flavone and other substances with good antioxidant activity, is not added with any chemical component, belongs to a pure natural and green raw material, is safe and environment-friendly, and meets the requirements of modern cosmetics on functionality and safety.

In order to achieve the above purpose, the invention provides the following technical scheme:

the invention provides a preparation method of pomegranate rind and schisandra chinensis fermentation stock solution, which comprises the following steps: fermenting pericarpium Granati and fructus Schisandrae chinensis to obtain pericarpium Granati and fructus Schisandrae chinensis fermentation stock solution; the fermentation starter comprises lactobacillus plantarum and streptococcus thermophilus, and the ratio of the viable count of the lactobacillus plantarum to the viable count of the streptococcus thermophilus is (0:1) - (1: 0).

Preferably, the pomegranate bark and the schisandra chinensis are powder.

Preferably, the mass ratio of the pomegranate bark powder to the schisandra fruit powder is (9:1) - (1: 9).

Preferably, the initial system of the fermentation further comprises glucose and water; the addition amount of the glucose is 0-10% of the total mass of the fermentation initial system; the ratio of the mass of the water to the mass sum of the pomegranate rind and the schisandra chinensis is (10:1) - (120: 1); the pH value of the fermentation initial system is 4-7.

Preferably, the viable count of the zymocyte is 10⁸～10¹⁰CFU/mL, the inoculation amount of the zymophyte is 0.2-1.0% by taking the volume of the fermentation initial system as a reference.

Preferably, the fermentation conditions are: the fermentation temperature is 33-41 ℃, and the fermentation time is 12-60 h.

The invention provides a pomegranate bark and schisandra chinensis fermentation stock solution, which is prepared by the preparation method in the technical scheme.

The invention provides application of the pomegranate bark and schisandra chinensis fermentation stock solution in cosmetics.

Preferably, the cosmetic is a whitening and/or anti-aging cosmetic.

Preferably, the fermentation raw liquid is used as it is or as a functional material added to cosmetics.

Has the advantages that:

the invention provides a preparation method of pomegranate rind and schisandra chinensis fermentation stock solution, which comprises the following steps: inoculating fermentation bacteria to pericarpium Granati and fructus Schisandrae chinensis, fermenting, centrifuging, and collecting supernatant to obtain pericarpium Granati and fructus Schisandrae chinensis fermentation stock solution; the fermentation bacteria comprise lactobacillus plantarum and streptococcus thermophilus, and the ratio of the number of the viable bacteria of the lactobacillus plantarum to the number of the viable bacteria of the streptococcus thermophilus is (0:1) - (1: 0). According to the method, the pomegranate rind and the schisandra chinensis are subjected to whole-plant fermentation by adopting the lactobacillus plantarum and the streptococcus thermophilus, all functional components and the activity of the plants are reserved, the loss of the active components is avoided, the content of the active components is improved, and the prepared fermentation stock solution has a good DPPH free radical removing function and a whitening and freckle removing effect; in addition, the preparation method of the invention uses pure natural plant fermentation, does not add any chemical component, belongs to pure natural and green raw materials, is safe and environment-friendly, and meets the requirements of modern cosmetics on functionality and safety.

Furthermore, the invention has mild fermentation conditions, further avoids the loss of active ingredients, improves the content of the active ingredients in the fermentation stock solution and improves the functional activity.

Furthermore, the fermentation stock solution prepared by the invention contains rich antioxidant active substances such as ellagic acid, lignan, flavone and the like, wherein the lignan not only can help a human body to resist oxygen free radicals, but also can directly promote the human body to produce the antioxidant substances by self, so that the problem of weak antioxidant capacity is fundamentally solved, and the antioxidant capacity of the human body is improved; in addition, ellagic acid can block melanin generation, and has effects of whitening skin, removing speckle and resisting oxidation.

Furthermore, the fermentation substrate used in the invention is pomegranate rind, and the part of pomegranate which can not be directly used is recycled, so that the recycling value of resources is improved, and a good effect is achieved.

Detailed Description

The invention provides a preparation method of pomegranate rind and schisandra chinensis fermentation stock solution, which comprises the following steps: fermenting pericarpium Granati and fructus Schisandrae chinensis to obtain pericarpium Granati and fructus Schisandrae chinensis fermentation stock solution; the fermentation starter bacteria comprise lactobacillus plantarum and streptococcus thermophilus, and the ratio of the viable count of the lactobacillus plantarum to the viable count of the streptococcus thermophilus is (0:1) - (1:0), more preferably (5:1) - (1:5), and even more preferably 1: 1. According to the invention, the lactobacillus plantarum and the streptococcus thermophilus are adopted to carry out whole-plant fermentation on the pomegranate rind and the schisandra chinensis, so that all functional components and the activity of the plants are reserved, the loss of the active components is avoided, the content of the active components is increased, and the prepared fermentation stock solution has a good DPPH free radical removing function and whitening and freckle removing effects; in addition, the preparation method of the invention uses pure natural plant fermentation, does not add any chemical component, belongs to pure natural and green raw materials, is safe and environment-friendly, and meets the requirements of modern cosmetics on functionality and safety.

According to the invention, pomegranate rind and schisandra chinensis are fermented, wherein the pomegranate rind and the schisandra chinensis are powder, and the particle size and the mesh number of the powder are preferably 30-100, more preferably 40-80, and even more preferably 50. The powdered pomegranate rind and the powdered schisandra chinensis can be used for fermentation more fully, so that the active ingredients in the raw materials are released fully. In the present invention, the mass ratio of the pomegranate bark powder to the schisandra fruit powder is preferably (9:1) to (1:9), more preferably (7:3) to (3:7), and still more preferably 1: 1. The pomegranate bark is rich in amino acid, VC and other nutritional ingredients, and also contains various trace elements such as copper, iron, zinc, manganese and the like, and components with oxidation resistance such as ellagitannin, ellagic acid, anthocyanin and the like, wherein the ellagitannin not only has strong capacity of removing free radicals and oxidation resistance, but also can block melanin generation, and has the effects of whitening, fading spots and oxidation resistance; the schisandra chinensis contains lignans, which not only can help the human body to resist oxygen free radicals, but also can directly promote the human body to produce antioxidant substances by self, thus fundamentally solving the problem of weak antioxidant capacity and improving the antioxidant capacity of the human body. According to the invention, the pomegranate bark and the schisandra chinensis are used for pure plant fermentation, and the prepared fermentation stock solution contains rich ellagic acid, lignanoid, flavone and other antioxidant active substances and has good antioxidant effect; in addition, the fermentation substrate used in the invention is pomegranate rind, and the part of pomegranate which can not be directly used is recycled, so that the recycling value of resources is improved, and a good effect is achieved.

In the present invention, the initial system of the fermentation preferably further comprises glucose and water; the addition amount of the glucose is 0-20% of the total mass of the fermentation initial system, preferably 5-15%, further preferably 10%, the fermentation initial system is all substances contained in the fermentation reaction, and the glucose provides an adequate carbon source for the zymophyte; the ratio of the mass of the water to the mass sum of the pomegranate rind and the schisandra chinensis is (10:1) - (120:1), more preferably (10:1) - (40:1), and still more preferably 20: 1; the pH value of the fermentation initial system is 4-7, and the preferable pH value is 6.

After the initial fermentation system is prepared, the initial fermentation system is preferably sterilized, the sterilization is preferably carried out under high pressure, and the sterilization of the initial system can ensure that the subsequent fermentation is not polluted by mixed bacteria, so that the fermentation fails.

After the initial fermentation system is sterilized, the invention adds zymophyte to the initial fermentation system, wherein the zymophyte comprises Lactobacillus plantarum and streptococcus thermophilus, and the Lactobacillus plantarum is preferably Lactobacillus plantarum CICC 21794(Lactobacillus plantarum); the Streptococcus thermophilus is preferably Streptococcus thermophilus CICC 20364(Streptococcus thalivarius). In the present invention, the ratio of the number of viable bacteria of lactobacillus plantarum to streptococcus thermophilus is (5:1) to (1:5), and more preferably 1: 1; in the present invention, the number of viable bacteria of said fermentation tubes is 10⁸～10¹⁰CFU/mL, more preferably 10⁹CFU/mL; the inoculation amount of the fermentation tubes is preferably 0.2-1.0%, more preferably 1%, based on the volume of the fermentation initiation system. In the invention, the fermentation temperature is preferably 33-41 ℃, and more preferably 39 ℃; the fermentation time is preferably 12-60 h, more preferably 24-48 h, and even more preferably 36 h. The lactobacillus plantarum and the streptococcus thermophilus are selected for mixed fermentation, the fermentation condition is mild, the loss of active ingredients is further avoided, the content of the active ingredients in the fermentation stock solution is improved, and the functional activity is improved.

After the fermentation is finished, the fermentation liquid is preferably sterilized, the zymophyte in the fermentation liquid is inactivated, and the fermentation is stopped. In the present invention, the fermentation sterilization mode is preferably high-pressure steam sterilization, and the sterilization condition is preferably sterilization at 121 ℃ for 15 min. After sterilization, the invention preferably centrifuges the sterilized fermentation liquor, and takes the supernatant fluid to obtain the pomegranate rind and schisandra chinensis fermentation stock solution. In the invention, the rotation speed of the centrifugation is preferably 4000-10000 rpm, and more preferably 10000 rpm; the time for centrifugation is preferably 5-15 min, and more preferably 10 min.

The invention provides a pomegranate bark and schisandra chinensis fermentation stock solution, which is prepared by the preparation method in the technical scheme. In the invention, the content of ellagic acid in the fermentation stock solution is preferably more than or equal to 90mg/g, the content of lignan is preferably more than or equal to 40mg/g, and the content of flavone is preferably more than or equal to 80 mg/g.

The invention provides application of the pomegranate bark and schisandra chinensis fermentation stock solution in cosmetics. In the present invention, the kind of the cosmetic is preferably a whitening and/or anti-aging cosmetic. In the present invention, the fermentation raw liquid is preferably used as it is or added to cosmetics as a functional material. In the invention, when the fermentation stock solution is used for preparing the anti-aging essence, the mass of the fermentation stock solution accounts for 3-8% of the total mass of the essence.

In order to further illustrate the present invention, the following examples are provided to describe a pomegranate rind and schisandra chinensis fermentation stock solution and a preparation method and application thereof in detail, but they should not be construed as limiting the scope of the present invention.

Example 1

Weighing 2.5g of pomegranate rind and schisandra chinensis powder respectively, mixing with 100mL of deionized water, adding 3g of food-grade glucose, and adding food-grade Na₂CO₃Adjusting the initial pH value to 6.0, sterilizing and cooling to obtain a fermentation initial system; adding plant lactobacillus bacteria liquid and streptococcus thermophilus liquid into the initial fermentation system for fermentation, wherein the viable count of the plant lactobacillus bacteria liquid and the streptococcus thermophilus liquid is 10⁹CFU/mL, the addition of the two bacterial liquids is 0.5mL, and the two bacterial liquids are cultured in a constant temperature shaking table at 39 ℃ for 36 hours; after the fermentation is finished, the obtained fermentation liquor is sterilized for 15min at 121 ℃ in a high-pressure steam sterilization pot. After sterilization, centrifuging the sterilized fermentation liquor for 10min at 4 ℃ under the condition of 10000r/min, and taking supernatant fluid to obtain pomegranate rind and schisandra chinensis fermentation stock solution.

Example 2

The preparation method is the same as example 1, except that the fermentation time is 24 h.

Example 3

The preparation method is the same as that of example 1, except that the glucose addition amount is 0%.

Example 4

The preparation method was the same as example 1, except that the amounts of the added Lactobacillus plantarum solution and Streptococcus thermophilus solution were 0.25 mL.

Example 5

The preparation method was the same as example 1, except that the temperature of the fermentation was 35 ℃.

Example 6

The preparation method was the same as example 1, except that 0.5mL of the plant lactobacillus solution was added.

Example 7

The preparation method was the same as example 1, except that only 0.5mL of Streptococcus thermophilus solution was added.

Comparative example 1

Weighing 2.5g of pomegranate bark and schisandra chinensis powder respectively, mixing with 100mL of ethanol with the volume percentage of 70%, and then carrying out ultrasonic treatment for 40min at the ultrasonic power of 400w and the ultrasonic temperature of 50 ℃ to obtain an alcohol extract of the pomegranate bark and the schisandra chinensis.

Comparative example 2

The preparation method is the same as example 1, except that only 5.0g of pomegranate rind powder is added.

Comparative example 3

The preparation method is the same as example 1, except that only 5.0g of schisandra chinensis powder is added.

Evaluation of Performance

1 measurement of content of antioxidant active substances in pomegranate peel and schisandra chinensis fermentation stock solution

1.1 measurement of ellagic acid content in pomegranate rind and Schisandra chinensis fermentation stock solution

The content of ellagic acid in the solutions prepared in examples 1 to 7 and comparative examples 1 to 3 was measured by spectrophotometry.

Preparing a standard curve: precisely weighing ellagic acid standard substance, and diluting with distilled water to obtain concentration gradient shown in Table 1; preparing 0.2mol/L NaOH solution; respectively sucking 2mol of standard product diluent and 2mol of NaOH solution, placing the standard product diluent and the 2mol of NaOH solution in a 100mL volumetric flask, uniformly mixing, and measuring the absorbance at 357 nm; making a standard curve to obtain a standard concentration equation, wherein the standard equation is as follows: Y-0.05179X-0.0029, R²0.99971. And (4) measuring the content of the ellagic acid in the sample. The sample testing method is the same as the standard solution testing method, and the solution is diluted by 10 times before testing; the test results are substituted into the equation to obtain the content of the ellagic acid, and the test results are shown in table 2.

TABLE 1 Absorbance of ellagic acid standards

TABLE 2 ellagic acid content of solutions prepared in examples 1 to 7 and comparative examples 1 to 3

And (4) analyzing results: as can be seen from Table 2, the ellagic acid content of the pomegranate rind and schisandra chinensis fermentation stock solutions obtained in examples 1 to 5 is up to 80mg/g, which is obviously improved compared with the alcohol extract solution and the single pomegranate rind fermentation solution in the comparative example 1. Examples 6 to 7 are single strain fermentation, the ellagic acid content of the obtained pomegranate rind and schisandra chinensis fermentation stock solution is reduced compared with examples 1 to 5, but is obviously improved compared with comparative example 1 by only ultrasonic treatment, which shows that the lactobacillus plantarum and streptococcus thermophilus used in the invention have the effect of obviously improving the ellagic acid after being fermented respectively. Since the content of the pomegranate rind in examples 1 to 7 is half of that of the fermentation substrate, the content of ellagic acid, which is the pomegranate rind active substance, detected in examples 1 to 7 is higher than that of comparative example 1, and is also significantly higher than 1/2, which is the detected content of ellagic acid, of comparative example 2 in which only the pomegranate rind is fermented. Therefore, the fermentation process is more beneficial to releasing the active ellagic acid in the pomegranate rind.

1.2 measurement of lignanoid content in pomegranate rind and schisandra chinensis fermentation stock solution

The content of lignans in the solutions prepared in examples 1 to 7 and comparative examples 1 to 3 was measured by a spectrophotometer method.

Preparing a standard curve: precisely weighing Schisandrin B standard substance, dissolving with methanol, and making into Schisandrin B standard substance solution with concentration gradient shown in Table 3; sucking 1mL of the solution into a test tube, heating the solution in a water bath at 70 ℃ to volatilize methanol, and adding 10% of the methanol to the solution respectively0.5mL of a chromophoric acid solution, 3.0mL of concentrated sulfuric acid and 1.5mL of distilled water; adding a plug, shaking, heating in boiling water bath for 30min, taking out, and cooling; measuring the absorbance at 570 nm; making a standard curve to obtain a standard equation Y which is 0.16826X-0.01374, R²＝0.99929。

The sample test method is the same as the standard solution test method; the test results are substituted into the equation to obtain the ellagic acid content, and the test results are shown in Table 4.

TABLE 3 Schizandrin B Standard substance Absorbance

TABLE 4 lignan content of solutions prepared in examples 1 to 7 and comparative examples 1 to 3

And (4) analyzing results: as can be seen from Table 4, the lignan content of the pomegranate bark and schisandra chinensis fermentation stock solutions in examples 1-5 is higher than 40mg/g, which is obviously improved compared with the lignan content of the alcohol extract solution in comparative example 1. Lignans are the active ingredient of schisandra chinensis, and are not present in pomegranate rind. The lignan content of the pomegranate rind and schisandra chinensis fermentation stock solutions obtained in examples 6 and 7 is slightly lower than that of the fermentation stock solutions obtained in examples 1 to 5. Since the content of schisandra fruit in examples 1 to 7 is half of the fermentation substrate, the content of lignan detected in examples 1 to 7 is higher than that of comparative example 1, and is also significantly higher than 1/2, which is the detected content of ellagic acid, of comparative example 2 in which only pomegranate rind is fermented. Therefore, the fermentation process is more beneficial to releasing the active lignan of the schisandra chinensis.

1.3 measurement of flavone and protein contents in pomegranate rind and schisandra chinensis fermentation stock solution

The content of flavone in the solutions prepared in examples 1 to 7 and comparative examples 1 to 3 was measured by a spectrophotometer method.

Preparing a standard curve: accurately weighing rutin standard substance, and diluting with 95% ethanol solution to obtain concentration gradient shown in Table 5; adding 5% NaNO₂1mL of the solution is fully shaken up and kept stand for 6 min; adding 10% Al (NO)₃)₃1mL of the solution is fully shaken up and kept stand for 6 min; adding 10mL of 4% NaOH solution and distilled water to a constant volume of 25mL, fully shaking up and standing for 15 min; measuring absorbance at 365 nm; making a standard curve and obtaining a standard equation Y which is 0.02646X-0.08407, R²0.99856. And (4) measuring the content of the flavone in the sample. The sample test method is the same as the standard solution test method; the test result is substituted into the equation to obtain the flavone content.

The content of protein in the solutions prepared in examples 1 to 7 and comparative examples 1 to 3 was determined by the Coomassie Brilliant blue method.

Preparing a standard curve: preparing 0.9% NaCl solution; preparing 0.1mg/mL standard protein solution by using bovine serum albumin; preparing 0.01% Coomassie brilliant blue solution by using 95% ethanol solution and concentrated phosphoric acid; setting a concentration gradient of the standard protein solution as shown in Table 6, proportioning 0.9% NaCl solution with corresponding volume to form 1mL of mixed solution, adding 4mL of 0.01% Coomassie brilliant blue solution, standing at room temperature for 3min, and measuring absorbance at 595 nm; making a standard curve to obtain a standard equation Y which is 0.00976X +0.0522, R²＝0.99768。

And (5) measuring the content of the sample protein liquid. The sample test method is the same as the standard solution test method; and substituting the test result into an equation to obtain the content of the protein liquid.

TABLE 5 rutin Standard Absorbance

TABLE 6 Absorbance of standard protein solution

TABLE 7 measurement results of flavone and protein contents of solutions prepared in examples 1 to 7 and comparative examples 1 to 3

And (4) analyzing results: compared with the contents of flavone and protein in the probiotic fermentation liquid in the examples 1-7 and the ultrasonic alcoholic extract in the comparative example 1, the contents of flavone and protein are improved after fermentation, which shows that the fermentation of lactobacillus plantarum and streptococcus thermophilus is favorable for enhancing the release or conversion of active substances such as flavone and protein in plant cells.

2. Evaluation of Oxidation resistance

The solutions prepared in examples 1 to 5 and comparative examples 1 to 3 were measured for their antioxidant ability by DPPH radical scavenging method, and the results are shown in Table 8. The specific experimental steps of DPPH free radical clearance rate are as follows:

preparing the concentration of 2 multiplied by 10 by absolute ethyl alcohol^-4Taking 2mL of prepared DPPH solution as mol/L (D0110, Iska Biotech Co., Ltd., Shanghai), putting the prepared DPPH solution into a test tube, adding 2mL of deionized water, shaking up for 4mL, and measuring the absorbance of the solution at 517nm to be A₀(ii) a Placing 2mL of the prepared DPPH solution into a test tube, adding 2mL of the reaction solution of the sample to be detected after dilution by 1000 times, standing in shade at 37 ℃ for 30min, and measuring the absorbance A at 517nm₁。

The clearance calculation formula is: clearance (%) ═ a₀-A₁)÷A₀×100％

Remarking: the greater the clearance rate, the greater the antioxidant capacity.

TABLE 8 DPPH clearance results for solutions prepared in examples 1-7 and comparative examples 1-3

And (4) analyzing results: as can be seen from table 8, the clearance rate of DPPH free radicals of the fermentation stock solutions of examples 1 and 2 after diluted 1000 times can still reach more than 90%, and the anti-oxidation effect is good and is significantly better than that of the ultrasonic alcoholic extract of comparative example 1.

Application example 1

The pomegranate rind and schisandra chinensis fermentation stock solution prepared in example 1 is used for preparing anti-aging essence, and the formula is shown in table 9.

TABLE 9 preparation formula table of fermented pomegranate rind and schisandra anti-aging essence

The preparation process comprises the following steps:

a. 85g of deionized water and 0.1g of carbomer were weighed in advance. Stirring deionized water at 600rpm until the carbomer does not spill out of the beaker, slowly pouring the weighed carbomer into the deionized water, and stirring at 600rpm for about 5 minutes.

b. Weighing the rest components, reducing stirring speed, adding the rest components into the uniformly dispersed carbomer solution, and supplementing water.

c. And after the system is uniformly dispersed, adding triethanolamine dropwise into the system until the pH value of the system is neutral and the system becomes viscous obviously.

The whole operation process can be carried out under the condition of no heating, and can also be properly heated at 50-70 ℃ to dissolve some indissolvable components. It should be noted that triethanolamine needs to be heated and then cooled to 45 ℃ before being added dropwise, and at high temperature, triethanolamine is volatile and has unstable chemical properties. In addition, the transparent gel prepared by the process contains bubbles, but the bubbles automatically disappear after standing for 30 min.

Questionnaire survey for fermented pomegranate rind and schisandra chinensis anti-aging essence

After determining the formula and the process of the anti-aging essence of fermented pomegranate rind and schisandra chinensis, a small sample body test questionnaire survey is carried out on the product, and the essence is used for 4 weeks without other efficacy cosmetics. The user makes an objective evaluation after use. All samples were 30 for women, the age required was 26 to 30 years, and the face had fine lines, blotches, and effective samples were 28 for oily skin 14, dry skin 14, and sensitive skin 2. Grading the product: the difference is 0 to 2.0, the average is 0.1 to 5.0, the good is 5.1 to 8.0, and the good is 8.1 to 10.0. The results of the investigation are as follows:

1. the skin of a person with sensitive skin feels uncomfortable after the product is tried twice, and the use is stopped.

2. The results of the investigation of the product by oily skin patients are shown in the following table:

TABLE 10 oily skin people using this product to score

3. The product is used by dry skin patients, and the investigation result is shown in the following table:

TABLE 11 Scoring of dry skin patients with this product

As can be seen from tables 10-11, both oily skin and dry skin have good and even good evaluation on skin feel, moisturizing, fine line repair and spot fading, and the spot fading effect is slightly weak.

According to the results of the above examples and application examples, the fermentation stock solution prepared by the preparation method of pomegranate bark and schisandra chinensis fermentation stock solution provided by the invention contains rich ellagic acid, lignan, flavone and other substances with good antioxidant activity, does not add any chemical component, belongs to a pure natural and green raw material, is safe and environment-friendly, and meets the requirements of modern cosmetics on functionality and safety.

Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.

Claims

1. A preparation method of pomegranate rind and schisandra chinensis fermentation stock solution is characterized by comprising the following steps: fermenting pericarpium Granati and fructus Schisandrae chinensis to obtain pericarpium Granati and fructus Schisandrae chinensis fermentation stock solution;

the fermentation starter comprises lactobacillus plantarum and streptococcus thermophilus, and the ratio of the viable count of the lactobacillus plantarum to the viable count of the streptococcus thermophilus is (0:1) - (1: 0).

2. The method of claim 1, wherein the pomegranate rind and the schisandra chinensis are powders.

3. The preparation method according to claim 1, wherein the mass ratio of the pomegranate bark to the schisandra fruit is (9:1) - (1: 9).

4. The method of claim 1, wherein the fermentation starter system further comprises glucose and water; the addition amount of the glucose is 0-10% of the total mass of the fermentation initial system; the ratio of the mass of the water to the mass sum of the pomegranate rind and the schisandra chinensis is (10:1) - (120: 1); the pH value of the fermentation initial system is 4-7.

5. The method according to claim 1, wherein the number of viable bacteria of the fermentation tubes is 10⁸～10¹⁰CFU/mL, the inoculation amount of the zymophyte is 0.2-1.0% by taking the volume of the fermentation initial system as a reference.

6. The method of claim 1, wherein the fermentation conditions are: the fermentation temperature is 33-41 ℃, and the fermentation time is 12-60 h.

7. A pomegranate rind and schisandra chinensis fermentation stock solution, which is obtained by the preparation method of any one of claims 1 to 6.

8. The use of the pomegranate rind and schisandra chinensis fermentation broth of claim 7 in cosmetics.

9. Use according to claim 8, characterized in that the cosmetic category is whitening and/or anti-ageing cosmetics.

10. Use according to claim 8, characterized in that the fermentation stock is used as such or added to cosmetics as a functional raw material.