CN108157712A - A kind of fermentation juice of my pomegranate and preparation method thereof - Google Patents

A kind of fermentation juice of my pomegranate and preparation method thereof Download PDF

Info

Publication number
CN108157712A
CN108157712A CN201711407932.8A CN201711407932A CN108157712A CN 108157712 A CN108157712 A CN 108157712A CN 201711407932 A CN201711407932 A CN 201711407932A CN 108157712 A CN108157712 A CN 108157712A
Authority
CN
China
Prior art keywords
pomegranate
juice
fermentation
preparation
lactobacillus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711407932.8A
Other languages
Chinese (zh)
Inventor
吕昌勇
徐致远
苏米亚
刘振民
应杰
沈玲
孙颜君
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Bright Dairy and Food Co Ltd
Bright Dairy and Food Co Ltd
Original Assignee
Shanghai Bright Dairy and Food Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Bright Dairy and Food Co Ltd filed Critical Shanghai Bright Dairy and Food Co Ltd
Priority to CN201711407932.8A priority Critical patent/CN108157712A/en
Publication of CN108157712A publication Critical patent/CN108157712A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135Bacteria or derivatives thereof, e.g. probiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/165Paracasei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/173Reuteri
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/175Rhamnosus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/231Lactis
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/517Bifidum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/533Longum

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

The invention discloses a kind of fermentation juices of my pomegranate and preparation method thereof, which includes the following steps:S1:By pomegranate raw material juice, reduced sugar and water after mixing, by filtering and sterilization obtains mixed liquor;S2:Inoculating starter when liquid to be mixed is cooled to preset temperature, and ferment under the preset temperature, obtain fermentation juice of my pomegranate;S3:Homogeneous micronization is carried out to fermentation juice of my pomegranate.Reduced sugar include but are not limited to it is one or more in glucose, galactolipin, lactose, fructose syrup, mass percent of the reduced sugar in mixed liquor be 0.5%~2%;Leavening includes but are not limited to probiotics, and the additive amount of probiotics is 1 × 106~1 × 109Cfu/mL, the time of fermentation is 12~96h.The no added stabilizer of fermentation juice of my pomegranate, stable system, clean taste, sweet and sour taste, slurry viscosity is low, good fluidity, it can directly apply or be applied to other products as dispensing, and the preparation method of the fermentation juice of my pomegranate is simple for process, be suitble to factory's large-scale production.

Description

A kind of fermentation juice of my pomegranate and preparation method thereof
Technical field
The present invention relates to food technology field, more particularly to a kind of fermentation juice of my pomegranate and preparation method thereof.
Background technology
Pomegranate is a kind of berry, full of nutrition, containing a large amount of organic acid, carbohydrate, protein, fat, vitamin and The minerals such as calcium, phosphorus, potassium.Juice of my pomegranate is predominantly sold or is fabricated to directly as agricultural product for the application of pomegranate at present Beverage.
At present, the research fermented to pomegranate is relatively fewer.The problem of juice of my pomegranate fermentation process is primarily present is formula It is complex, and feedstock processing and production technology are complex, industrialized production has certain difficulty or due to pomegranate What juice contained in itself can be directly less using carbon source and nitrogen source, needs to be fermented with specific microorganism.
Therefore, still lack a kind of formula at present simply, strain is adaptable, and the ferment making that process operability is strong Method, this is those skilled in the art's urgent problem to be solved.
Invention content
An embodiment of the present invention provides a kind of fermentation juice of my pomegranate and preparation method thereof, the no added stabilizations of fermentation juice of my pomegranate Agent, stable system, clean taste, sweet and sour taste, slurry viscosity is low, good fluidity, can directly apply or be applied to as dispensing Other products, and the preparation method of the fermentation juice of my pomegranate is simple for process, is suitble to factory's large-scale production.
In a first aspect, an embodiment of the present invention provides a kind of fermentation juice of my pomegranate preparation method, this method includes following step Suddenly:
S1:By pomegranate raw material juice, reduced sugar and water after mixing, by filtering and sterilization obtains mixed liquor;
S2:Inoculating starter when liquid to be mixed is cooled to preset temperature, and ferment under the preset temperature, it obtains Ferment juice of my pomegranate.
The preparation method of juice of my pomegranate of the present invention does not need to the additives such as addition stabilizer, preservative, effectively reduces preparation The flow of method does not need to ferment using yeast, will not generate fermented glutinous rice flavor, but has taste and the fermentation of juice of my pomegranate simultaneously Flavor, mouthfeel alcohol just, simultaneously because this method suitable factory large-scale production simple for process.
Preferably, the pomegranate raw material juice includes one or both of Carthaginian apple raw juice and concentrated granada juice.
Preferably, the content of the pomegranate raw material juice is 50% Carthaginian apple raw juice~2.5 times concentrated pomegranate Normal juice.
Preferably, the reduced sugar include but are not limited to one kind in glucose, galactolipin, lactose, fructose syrup or It is a variety of;Mass percent of the reduced sugar in mixed liquor is 0.5%~2%.
The present invention adds in a small amount of reduced sugar and plays the role of excitation during the fermentation and accelerate fermenting speed.
Preferably, it is 90~95 DEG C that required temperature is sterilized in step S1, and required time is 5~10min.
Preferably, preset temperature is 25~45 DEG C in step S2.
The present invention is fermented under mesophilic condition, is assigned while nutriment intrinsic in Carthaginian apple raw juice is kept Fermented product nutrition and ferment local-flavor, effectively avoiding since fermentation temperature is higher owes, the fermentation juice of my pomegranate mouthfeel of preparation The problem of good.
Preferably, the leavening includes but are not limited to probiotics;The additive amount of the probiotics is 1 × 106~1 ×109Cfu/mL, wherein cfu/mL are number of viable in every milliliter of mixed liquor;The time of fermentation is 12~96h.
Preferably, the probiotics for bifidobacterium bifidum, bifidobacterium longum, lactobacillus acidophilus, Lactobacillus paracasei, One or more of lactobacillus reuteri, lactobacillus plantarum, Lactobacillus rhamnosus and Lactococcus lactis.
Preferably, the probiotics is lactobacillus plantarum, and the lactobacillus plantarum is lactobacillus plantarum ST-III.
In the present invention using lactobacillus plantarum especially with lactobacillus plantarum ST-III as leavening, be both to ferment Agent is probiotics again, can not only be fermented to juice of my pomegranate, and probiotics directly improves host (eater) Tiny ecosystem and puts down Weighing apparatus plays beneficial effect.In addition to this, lactobacillus plantarum ST-III has effects that reduce cholesterol and high-efficient fat reducing, makes hair Ferment juice of my pomegranate has health-care efficacy, and with the safety that can be eaten for a long time.It cannot for fat or other reasons The crowd of polysaccharide was eaten, which does not have to not only worry to cause due to eating excessive sugar equally suitable for drinking Obesity, and at the same time hyperlipidemia can be improved, the health status of inside and outside minor, comprehensively improvement eater, prevention cardiovascular diseases, The generation of the metabolic disturbance diseases such as diabetes improves the quality of life of eater.
Preferably, the leavening further includes lactobacillus bulgaricus and/or streptococcus thermophilus;The bulgarian milk The additive amount of bacillus and/or streptococcus thermophilus is 1 × 106~1 × 109Cfu/mL, wherein cfu/mL is in every milliliter of mixed liquors Number of viable.
It can accelerate fermenting speed by adding in lactobacillus bulgaricus and/or streptococcus thermophilus in the present invention, save Fermentation time.
Preferably, it further includes upon step s 2:
S3:Homogeneous micronization is carried out to fermentation juice of my pomegranate;
The temperature of the homogeneous is the fermentation temperature in step S2;The homogeneous includes level-one homogeneous and/or two level is equal Matter;The pressure of level-one homogeneous and double-stage homogenization and be 15~25MPa.
The present invention carries out homogenization for fermentation juice of my pomegranate, can enhance the stable state of fermentation juice of my pomegranate, avoid sending out Ferment juice of my pomegranate bleed generates the problems such as precipitation, and fermentation juice of my pomegranate is caused to go bad and cannot eat.
Second aspect, an embodiment of the present invention provides a kind of fermentation juice of my pomegranate, the fermentation juice of my pomegranate in first aspect by appointing The preparation method of fermentation juice of my pomegranate described in one is made.
The present invention at least has the advantages that compared with prior art:
Preparation method raw material type in the present invention is few, and formula is simple, simple production process, and factory is suitble to give birth on a large scale Production;Using made from preparation method of the present invention ferment juice of my pomegranate without yeast ferment, no fermented glutinous rice flavor, mouthfeel alcohol just, it is sour-sweet can Mouthful, ferment local-flavor is strong, and not only the taste with juice of my pomegranate also has ferment local-flavor, and color, smell and taste are all good;Slurry viscosity is low, mobility It is good, it can directly apply or be applied to other products as dispensing;The body of fermentation juice of my pomegranate in the case where no stabilizer adds System still has high stability;Fermentation juice of my pomegranate refrigeration shelf-life can reach 1 month, freezing shelf-life can reach 24 months, the shelf-life after sterilization processing can reach 12 months under normal temperature condition, and refrigerate and freezen protective fermentation Viable count in juice of my pomegranate is maintained at 107More than cfu/mL, stability and microbiological indicator are splendid, greatly extend guarantor The matter phase expands sale radius.
What deserves to be explained is streptococcus thermophilus, lactobacillus bulgaricus, bifidobacterium bifidum, bifidobacterium longum, acidophilus Lactobacillus, Lactobacillus paracasei, lactobacillus reuteri, Lactobacillus rhamnosus and Lactococcus lactis are purchased from Hansen Corp. of section.Plant Lactobacillus ST-III (CGMCC No.0847) is purchased from Shanghai Bright Dairy & Food Co., Ltd..
Specific embodiment
Purpose, technical scheme and advantage to make the embodiment of the present invention are clearer, below in the embodiment of the present invention Technical solution is clearly and completely described, it is clear that described embodiment is part of the embodiment of the present invention rather than complete The embodiment in portion, based on the embodiments of the present invention, those of ordinary skill in the art are in the premise for not making creative work Lower all other embodiments obtained, shall fall within the protection scope of the present invention.
An embodiment of the present invention provides a kind of preparation method for the juice of my pomegranate that ferments, this method may comprise steps of:
S1:By pomegranate raw material juice, reduced sugar and water after mixing, by filtering and sterilization obtains mixed liquor.
S2:Inoculating starter when liquid to be mixed is cooled to preset temperature, and ferment under the preset temperature, it obtains Ferment juice of my pomegranate.
Effectively fermentation juice of my pomegranate is preserved for convenience, which further comprises:
S3:To ferment juice of my pomegranate homogeneous.
Specific steps in the label and label of above-mentioned S1, S2, S3, which are not used in, limits each step in preparation method Sequentially, each step in method, as long as in logic rationally, the sequence of each step can change.
Above-mentioned leavening, includes but are not limited to probiotics.Probiotics is selected from the permission of national correlation department regulation and is eating It is used in conduct industry and generally acknowledges one or more of strain with prebiotic function, preferably bifidobacterium bifidum (Bifidobacterium bifidum), bifidobacterium longum (Bifidobacterium longum), lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus paracasei (Lactobacillus paracasei), Luo Yishi breast bars Bacterium (Lactobacillus reuteri), lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus rhamnosus One or more of (Lactobacillus rhamnosus) and Lactococcus lactis (Lactococcus lactis), It is more preferably one or more of Lactobacillus paracasei, lactobacillus plantarum, Lactobacillus rhamnosus and lactobacillus acidophilus, Most preferably it is lactobacillus plantarum.Above-mentioned lactobacillus plantarum is preferably lactobacillus plantarum ST-III.The additive amount of probiotics compared with It is 1 × 10 goodly6~1 × 109Cfu/mL is more preferably 2 × 106~6 × 107Cfu/mL, above-mentioned cfu/mL are every milliliter Number of viable in mixed liquor.
Meanwhile can also be added in leavening lactobacillus bulgaricus (Lactobacillus bulgaricus) and/or Streptococcus thermophilus (Streptococcus thermophilus);It is more preferably lactobacillus bulgaricus, is most preferably Bao Jiali Sub- lactobacillus and streptococcus thermophilus.The additive amount of leavening is preferably 1 × 106~1 × 109Cfu/mL, more preferably for 2 × 106~6 × 107Cfu/mL is most preferably 9 × 106Cfu/mL, above-mentioned cfu/mL are the viable count in every milliliter of mixed liquor Amount.
Above-mentioned pomegranate raw material juice, including one or both of Carthaginian apple raw juice (juice of my pomegranate stoste) and concentrated granada juice.Compared with It is one or both of Carthaginian apple raw juice and concentrated granada juice goodly;Best is Carthaginian apple raw juice;The content of Carthaginian apple raw juice is more preferably It is most preferably 93.8% for 80%~2 times of concentration.Mass percent of the pomegranate raw material juice in mixed liquor for 50%~ 95%.
Above-mentioned reduced sugar includes one or more in glucose, galactolipin, lactose, fructose syrup.Preferably grape One or more of sugar, lactose and fructose syrup;Most preferably it is glucose.Reduced sugar additive amount is preferably 0.5% ~2%, it is more preferably 1.0~1.5%.
Uniformly mixed in above-mentioned steps S1 is after raw material is mixed by proportioning, to stir or recycle under 20~80 DEG C of environment 10~30 minutes, it is preferred that stir or recycle 12~20 minutes under 45~55 DEG C of environment.
Filtering in step S1 refers to filters commonly used in the art, it is therefore an objective to remove impurity, the fruit table in raw material Skin, fiber or a small amount of pomegranate fruit for containing etc..
Sterilization in step S1 can be 90~95 DEG C and handle 5~10 minutes that preferably 92~94 DEG C handle 7~9 points Clock.
Preset temperature is preferably 25~45 DEG C in step S2, is more preferably 28~34 DEG C.Fermentation time is preferably 12 ~96 hours, more preferably 24~72 hours.
It can also include fermentation juice of my pomegranate additive, above-mentioned fermentation juice of my pomegranate addition in mixed liquor in above-mentioned steps S1 Object includes flavor substance, pigment, fruit juice, prebiotics, PURE WHEY, milk protein powder, one kind in maltodextrin or more Kind.It is preferably one or more in fruit juice and prebiotics, it is more preferably fruit juice.Above-mentioned flavor substance is preferably eaten Use essence.Prebiotics are preferably inulin.Ferment juice of my pomegranate additive additive amount for 0~12%, preferably 0.5~ 12%, it is more preferably 2~8%.
Step S3 can be will ferment juice of my pomegranate homogeneous or use mulser refinement.By verify fermentation juice of my pomegranate into Row refines or using mulser, fermentation juice of my pomegranate is sheared by emulsification pump, refines fermentation juice of my pomegranate, so as to make hair Ferment juice of my pomegranate have better mobility and make fermentation juice of my pomegranate in shelf-life internal stability more preferably.
The mode of above-mentioned refinement preferably uses homogenization.Above-mentioned homogeneous is preferably comprised level-one homogeneous, more preferably wraps Include level-one homogeneous and double-stage homogenization.The pressure summation of level-one homogeneous and double-stage homogenization is preferably 15~25MPa, more preferably for 19~21MPa.
It can be refrigerated, freezed or sterilization processing to be preserved to fermentation juice of my pomegranate.The temperature of freezen protective It is -70 to -10 DEG C.It is stored refrigerated to refer to be 2 to 10 DEG C.Sterilization refers to that further sterilization can be 85~137 DEG C of processing 4s~5min, preferably 90~95 DEG C 10~30s of processing.Sterilization, enzyme deactivation under certain conditions, avoid harmful microorganism Pollution.Sterilization postcooling to temperature is preferably 4~30 DEG C, is more preferably 15~25 DEG C so that fermentation juice of my pomegranate obtained Cool to required temperature.
Before freezen protective, first fermentation juice of my pomegranate can also be cooled down, it is preferable to be cooled to temperature for the temperature of cooling Ground is 4~30 DEG C, is more preferably 15~25 DEG C.Fermentation juice of my pomegranate after cooling can also carry out canned.Filling can use fills Installing is standby to be filled in clean container, and preferable container is plastic barrel, and filling volume is less than 90%.
Fermentation juice of my pomegranate refrigeration, freezen protective, viable count keep viable bacteria 107More than cfu/mL.Stored refrigerated guarantees the quality Phase can reach 1 month, and the shelf-life of freezen protective can reach 24 months, and the shelf-life after sterilization processing at normal temperatures can reach 12 months.
The preparation method of above-mentioned fermentation juice of my pomegranate, by using the raw material centainly matched, by raw material by sterilizing, fermenting, The series of steps of homogeneous, refrigeration, freezing or sterilization processing etc., so as to obtain a kind of fermentation juice of my pomegranate, fermentation juice of my pomegranate warp Refrigeration, freezen protective are crossed, viable count keeps viable bacteria 107More than cfu/mL, by refrigerating, freezing or sterilization processing shelf-life point It not can reach 1 month, 24 months and room temperature 12 months, stability and microbiological indicator are good, greatly extend the shelf-life, expand Big sale radius.
In addition, the fermented fruits and vegetables juice as made from above-mentioned preparation method, without stabilizer, system is more stable, clean taste, acid Sweet tea is tasty, and slurry viscosity is low, good fluidity, can directly apply or be applied to other products as dispensing, and simple for process, It is suitble to factory's large-scale production.
In addition, it also shows in conjunction with the embodiments, using the fermented fruits and vegetables juice acid obtained by above-mentioned fermented fruits and vegetables juice composition Sweet tea is tasty, and existing common pomegranate on the market is superior in appearance luster, structural state, mouthfeel, flavor, sour-sweet degree etc. Juice has good color and luster, stability, taste and flavor.
In addition, by filtering and the optimization of the technological parameter of homogeneous etc., the appearance luster of the juice of my pomegranate that ferments, structural state, Mouthfeel, flavor, sour-sweet degree are further promoted, and are much better than existing juice of my pomegranate.
In another particular embodiment of the invention, a kind of fermentation juice of my pomegranate is additionally provided, by any of the above-described kind of fermentation pomegranate The preparation method of juice is made.
Viable bacteria content in above-mentioned fermentation juice of my pomegranate is more than 107cfu/mL.The upper limit is not provided with herein for viable bacteria content, This is because in the art, the requirement of lower limiting value can be usually set to viable bacteria content, but generally will not determination of the upper limit value. The characteristics of this is due to fermentation to a certain extent is caused, the growth of viable bacteria content impossible no maximum within the regular hour, Generally reaching certain peak value can begin to decline later.Therefore, even if indefinite specific upper limit value herein, for this field It is also clear for technical staff.
Above-mentioned specific embodiment is further illustrated below by embodiment, but therefore do not limit the present invention to institute Among the scope of embodiments stated.Test method without specific conditions in the following example, according to conventional methods and conditions, Or it is selected according to product manual.
Embodiment 1
1st, it matches
Table 1
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 30min at 20 DEG C by the proportioning of table 1, filtered, 90 DEG C are killed Bacterium 5min obtains mixed liquor, and wherein juice of my pomegranate content is 40%;
(2) mixed liquor is cooled to 25 DEG C, adds leavening according to the proportioning of table 1, ferment at 25 DEG C, 96 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate by 54 DEG C, 15Mpa homogeneous, 85 DEG C of 300s sterilization processings are cooled to 4 DEG C, filling, can Carry out room temperature transport and storage.
After testing, after placement after 12 months, mould, saccharomycete, Escherichia coli, pathogenic bacteria do not detect;Products obtained therefrom is used It is appraised through comparison in taste.
Embodiment 2
1st, it matches
Table 2
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 28min at 30 DEG C by the proportioning of table 2, filtered, 91 DEG C are killed Bacterium 5min obtains mixed liquor, and wherein juice of my pomegranate content is 50%;
(2) mixed liquor is cooled to 26 DEG C, adds leavening according to the proportioning of table 2, ferment at 26 DEG C, 90 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) fermentation juice of my pomegranate by emulsification pump is refined, is cooled to 15 DEG C, filling, transport and storage under the conditions of 10 DEG C.
After testing, after placement after 2 months, lactic acid bacteria number 6.7*107Cfu/mL, mould, saccharomycete, Escherichia coli, Pathogenic bacteria meet beverage national standard microorganism limitation;Products obtained therefrom is appraised through comparison for taste.
Embodiment 3
1st, it matches
Table 3
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 25min at 40 DEG C by the proportioning of table 3, filtered, 92 DEG C are killed Bacterium 6min obtains mixed liquor, and wherein juice of my pomegranate content is 70%;
(2) mixed liquor is cooled to 27 DEG C, adds leavening according to the proportioning of table 3, ferment at 27 DEG C, 80 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) fermentation juice of my pomegranate is passed through, 15Mpa level-one homogeneous, 3Mpa double-stage homogenizations are cooled to 16 DEG C, filling, at 2 DEG C Deepfreeze transports and storage.
After testing, after placement after 2 months, lactic acid bacteria number 9.11*107Cfu/mL, mould, saccharomycete, in large intestine bar Bacterium, pathogenic bacteria meet beverage national standard microorganism limitation;Products obtained therefrom is appraised through comparison for taste.
Embodiment 4
1st, it matches
Table 4
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 20min at 45 DEG C by the proportioning of table 4, filtered, 92 DEG C are killed Bacterium 7min obtains mixed liquor, and wherein juice of my pomegranate content is 80%;
(2) mixed liquor is cooled to 28 DEG C, adds leavening according to the proportioning of table 4, ferment at 28 DEG C, 72 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate by 57 DEG C, 15Mpa level-ones homogeneous, 4Mpa double-stage homogenizations, 90 DEG C, 30s sterilization processings, It is cooled to 18 DEG C, it is filling, room temperature transport and storage can be carried out.
After testing, after placement after 12 months, mould, saccharomycete, Escherichia coli, pathogenic bacteria do not detect;Products obtained therefrom is used It is appraised through comparison in taste.
Embodiment 5
1st, it matches
Table 5
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 15min at 50 DEG C by the proportioning of table 5, filtered, 93 DEG C are killed Bacterium 8min obtains mixed liquor, and wherein juice of my pomegranate content is 93.8%;
(2) mixed liquor is cooled to 30 DEG C, adds leavening according to the proportioning of table 5, ferment at 30 DEG C, 48 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate by 60 DEG C, 18Mpa level-one homogeneous, 2Mpa double-stage homogenizations, 90 DEG C of 15s sterilization processings, It is cooled to 20 DEG C, it is filling, room temperature transport and storage can be carried out.
After testing, after placement after 12 months, mould, saccharomycete, Escherichia coli, pathogenic bacteria do not detect;Products obtained therefrom is used It is appraised through comparison in taste.
Embodiment 6
1st, it matches
Table 6
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 12min at 55 DEG C by the proportioning of table 6, filtered, 94 DEG C are killed Bacterium 9min obtains mixed liquor, and wherein juice of my pomegranate content is 1.2 times of stostes;
(2) mixed liquor is cooled to 34 DEG C, adds leavening according to the proportioning of table 6, ferment at 34 DEG C, 24 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate by 62 DEG C, 18Mpa level-ones homogeneous, 3Mpa double-stage homogenizations, 95 DEG C of 10s sterilization processings, It is cooled to 22 DEG C, it is filling, room temperature transport and storage can be carried out.
After testing, after placement after 12 months, mould, saccharomycete, Escherichia coli, pathogenic bacteria do not detect;Products obtained therefrom is used It is appraised through comparison in taste.
Embodiment 7
1st, it matches
Table 7
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 11min at 65 DEG C by the proportioning of table 7, filtered, 94 DEG C are killed Bacterium 9min obtains mixed liquor, and wherein juice of my pomegranate content is 2 times of stostes;
(2) mixed liquor is cooled to 38 DEG C, adds leavening according to the proportioning of table 7, ferment at 38 DEG C, 48 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate by 63 DEG C, 22Mpa homogeneous is cooled to 24 DEG C, filling, is carried out in subzero 10 DEG C of conditions Transport and storage.
After testing, after placement after 24 months, lactic acid bacteria number 4.5*107Cfu/mL, mould, saccharomycete, Escherichia coli, Pathogenic bacteria do not detect;Products obtained therefrom is appraised through comparison for taste.
Embodiment 8
1st, it matches
Table 8
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 10min at 70 DEG C by the proportioning of table 8, filtered, 95 DEG C are killed Bacterium 10min obtains mixed liquor, and wherein juice of my pomegranate content is 2.5 times of stostes;
(2) mixed liquor is cooled to 38 DEG C, adds leavening according to the proportioning of table 8, ferment at 40 DEG C, after 30 hours Stuck fermentation obtains fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate by 64 DEG C, 20Mpa level-one homogeneous, 3Mpa double-stage homogenizations are cooled to 25 DEG C, filling, It is transported and is stored in subzero 70 DEG C of conditions.
After testing, after placement after 24 months, lactic acid bacteria number 5.7*107Cfu/mL, mould, saccharomycete, Escherichia coli, Pathogenic bacteria do not detect;Products obtained therefrom is appraised through comparison for taste.
Embodiment 9
1st, it matches
Table 9
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 10min at 80 DEG C by the proportioning of table 9, filtered, 95 DEG C are killed Bacterium 10min obtains mixed liquor, and wherein juice of my pomegranate content is 3 times of stostes;
(2) mixed liquor is cooled to 45 DEG C, adds leavening according to the proportioning of table 9, ferment at 45 DEG C, 12 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate by 66 DEG C, 21Mpa level-one homogeneous, 4Mpa double-stage homogenizations, 137 DEG C of 4s sterilization processings, It is cooled to 30 DEG C, it is filling, room temperature transport and storage can be carried out.
After testing, after placement after 12 months, mould, saccharomycete, Escherichia coli, pathogenic bacteria do not detect;Products obtained therefrom is used It is appraised through comparison in taste.
Comparative example 1
1st, it matches
With embodiment 1.
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 30min by the proportioning of table 1 at 20 DEG C, no filtering, 90 DEG C 5min is sterilized, obtains mixed liquor;
(2) mixed liquor is cooled to 25 DEG C, adds leavening according to the proportioning of table 1, ferment at 25 DEG C, 96 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate through 85 DEG C of 300s sterilization processings, be cooled to 4 DEG C, it is filling, room temperature transport and storage can be carried out It deposits.
After testing, after placement after 12 months, mould, saccharomycete, Escherichia coli, pathogenic bacteria do not detect;Products obtained therefrom is used It is appraised through comparison in taste.
Fermentation juice of my pomegranate obtained by comparative example 1 in dispensing without filtering, without homogeneous after fermentation, it is filling after at 19 days Start bleed, state labile and lasting aggravation, occur more serious precipitation after 25 days, therefore test and terminate.
Comparative example 2
1st, it matches
Table 10
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 30min at 20 DEG C by the proportioning of table 10, filtered, 90 DEG C 5min is sterilized, obtains mixed liquor;
(2) mixed liquor is cooled to 25 DEG C, adds leavening according to the proportioning of table 10, ferment at 25 DEG C, 96 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate by 54 DEG C, 15Mpa homogeneous, 85 DEG C of 300s sterilization processings are cooled to 4 DEG C, filling, can Carry out room temperature transport and storage.
After testing, after placement after 12 months, mould, saccharomycete, Escherichia coli, pathogenic bacteria do not detect;Products obtained therefrom is used It is appraised through comparison in taste.
Comparative example 3
1st, it matches
Table 11
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 10min at 80 DEG C by the proportioning of table 11, filtered, 95 DEG C 10min is sterilized, obtains mixed liquor, wherein juice of my pomegranate content is 3.57 times of Normal juice;
(2) mixed liquor is cooled to 45 DEG C, adds leavening according to the proportioning of table 11, ferment at 45 DEG C, 12 hours Stuck fermentation afterwards obtains fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate by 66 DEG C, 21Mpa level-one homogeneous, 4Mpa double-stage homogenizations, 137 DEG C of 4s sterilization processings, It is cooled to 30 DEG C, it is filling, room temperature transport and storage can be carried out.
Through experiment, since pomegranate inspissated juice content is higher in the design, when carrying out dispensing, viscosity is larger, and loss is larger, And pipeline easily blocks, and does not have production operability.
Comparative example 4
1st, it matches
Table 12
2nd, method
(1) pomegranate raw material, reduced sugar and water are stirred 30min at 20 DEG C by the proportioning of table 12, filtered, 90 DEG C 5min is sterilized, obtains mixed liquor;
(2) mixed liquor is cooled to 25 DEG C, adds leavening according to the proportioning of table 12, ferment at 25 DEG C, 120 is small When after stuck fermentation, obtain fermentation juice of my pomegranate;
(3) will fermentation juice of my pomegranate by 54 DEG C, 15Mpa homogeneous, 85 DEG C of 300s sterilization processings are cooled to 4 DEG C, filling, can Carry out room temperature transport and storage.
Through overtesting, no added reduced sugar in the design, after fermentation 120 hours, lactic acid bacteria number does not increase significantly Add, ph lowers without apparent, shows not ferment, and shows an important factor for reduced sugar comparatively fast ferments for fermentation juice of my pomegranate.
Comparative example 5
It is compared with buying commercially available juice of my pomegranate
To juice of my pomegranate and the fermentation juice of my pomegranate progress obtained of comparative example 2, comparative example 5 of fermenting made from embodiment 1-9 Taste and flavor judges experiment, matter of uniformly going bail for end of term product.Examination by sensory organs project is:Appearance luster, structural state, mouthfeel, Flavor, sour-sweet degree, sensory evaluation scores standard are as shown in table 13.This experiment number is participated in as 50 people, sensory evaluation scores project is averaged Value, score is higher, represents more close to the best features of product, and counts tested personnel to the degree of liking of product, sensory evaluation scores Result it is as shown in table 14.
13 sensory evaluation scores standard of table
14 results of sensory evaluation of table
Results of sensory evaluation shows:Fermentation juice of my pomegranate obtained by the embodiment of the present invention is in original taste and flavor On the basis of, the flavor of fermentation is imparted, mouthfeel is more soft, naturally, the state of product, taste flavor are good.
From embodiment 1-9 and 5 effect data of comparative example relatively from the point of view of, consumer is to preparation method provided by the present invention Obtained fermentation juice of my pomegranate preference degree is slightly better than to existing commercially available juice of my pomegranate, is being mainly reflected in mouthfeel more alcohol just, and ferment stone Pomegranate juice taste is denseer, and acidity is more preferably natural.
Comparison example 2, although can successfully ferment, juice of my pomegranate content is relatively low, therefore the side such as color, flavor, mouthfeel Face is more flat, and sensory scores are relatively low.
Fermentation juice of my pomegranate composition provided by the present invention, fermentation juice of my pomegranate and preparation method thereof have been carried out in detail above It is thin to introduce.Specific case used herein is expounded the principle of the present invention and embodiment, and above example is said The bright method and its core concept for being merely used to help understand the present invention.It should be pointed out that the ordinary skill for the art For personnel, without departing from the principle of the present invention, can also to the present invention some improvement and modification can also be carried out, these change It is also fallen within the protection scope of the claims of the present invention into modification.

Claims (10)

1. a kind of preparation method for the juice of my pomegranate that ferments, which is characterized in that the preparation method includes the following steps:
S1:By pomegranate raw material juice, reduced sugar and water after mixing, by filtering and sterilization obtains mixed liquor;
S2:Inoculating starter when liquid to be mixed is cooled to preset temperature, and ferment under the preset temperature, obtain fermentation stone Pomegranate juice.
2. the preparation method of fermentation juice of my pomegranate according to claim 1, which is characterized in that the pomegranate raw material juice includes stone One or both of pomegranate Normal juice and concentrated granada juice;The content of the pomegranate raw material juice is dense for 50% Carthaginian apple raw juice~2.5 times Contracting Carthaginian apple raw juice.
3. the preparation method of fermentation juice of my pomegranate according to claim 1, which is characterized in that the reduced sugar is included but not only It is limited to one or more in glucose, galactolipin, lactose, fructose syrup;Quality percentage of the reduced sugar in mixed liquor Number is 0.5%~2%.
4. the preparation method of fermentation juice of my pomegranate according to claim 1, which is characterized in that required temperature is sterilized in step S1 It is 90~95 DEG C to spend, and required time is 5~10min.
5. the preparation method of fermentation juice of my pomegranate according to claim 1, which is characterized in that preset temperature is 25 in step S2 ~45 DEG C.
6. the preparation method of fermentation juice of my pomegranate according to claim 1, which is characterized in that the leavening is included but not only It is limited to probiotics;The additive amount of the probiotics is 1 × 106~1 × 109Cfu/mL, wherein cfu/mL is in every milliliter of mixed liquors Number of viable;The time of fermentation is 12~96h.
7. the preparation method of fermentation juice of my pomegranate according to claim 6, which is characterized in that the probiotics is not tally bifid Bacillus, bifidobacterium longum, lactobacillus acidophilus, Lactobacillus paracasei, lactobacillus reuteri, lactobacillus plantarum, Lactobacillus rhamnosus One or more of with Lactococcus lactis.
8. the preparation method of fermentation juice of my pomegranate according to claim 6, which is characterized in that the leavening further includes guarantor and adds Leah lactobacillus and/or streptococcus thermophilus;The additive amount of the lactobacillus bulgaricus and/or streptococcus thermophilus is 1 × 106~ 1×109Cfu/mL, wherein cfu/mL are number of viable in every milliliter of mixed liquor.
9. the preparation method of fermentation juice of my pomegranate according to claim 1, which is characterized in that further include upon step s 2:
S3:Homogeneous micronization is carried out to fermentation juice of my pomegranate;
The temperature of the homogeneous is the fermentation temperature in step S2;The homogeneous includes level-one homogeneous and/or double-stage homogenization;Level-one The pressure of homogeneous and double-stage homogenization and be 15~25MPa.
10. a kind of fermentation juice of my pomegranate, which is characterized in that the fermentation juice of my pomegranate is by any fermentation pomegranates of claim 1-9 The preparation method of juice is made.
CN201711407932.8A 2017-12-22 2017-12-22 A kind of fermentation juice of my pomegranate and preparation method thereof Pending CN108157712A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711407932.8A CN108157712A (en) 2017-12-22 2017-12-22 A kind of fermentation juice of my pomegranate and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711407932.8A CN108157712A (en) 2017-12-22 2017-12-22 A kind of fermentation juice of my pomegranate and preparation method thereof

Publications (1)

Publication Number Publication Date
CN108157712A true CN108157712A (en) 2018-06-15

Family

ID=62523639

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201711407932.8A Pending CN108157712A (en) 2017-12-22 2017-12-22 A kind of fermentation juice of my pomegranate and preparation method thereof

Country Status (1)

Country Link
CN (1) CN108157712A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108925982A (en) * 2018-07-03 2018-12-04 淮北师范大学 A kind of preparation method of high anti-oxidation activity pomegranate ferment
CN113875919A (en) * 2021-10-27 2022-01-04 陕西师范大学 Fermented pomegranate juice with blood pressure reducing function and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101606737A (en) * 2009-07-07 2009-12-23 张建伟 Pomegranate fermented beverage and method for making
CN102309042A (en) * 2010-12-04 2012-01-11 赵敏 Fresh pomegranate probiotic beverage and preparation method thereof
CN105167036A (en) * 2015-06-09 2015-12-23 青岛博恩高科生物技术有限公司 Pomegranate juice beverage and preparation method thereof
CN105558731A (en) * 2016-01-15 2016-05-11 福建绿泉食品有限公司 Fruit and vegetable juice based on multi-bacterium combined fermenting technology and preparing method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101606737A (en) * 2009-07-07 2009-12-23 张建伟 Pomegranate fermented beverage and method for making
CN102309042A (en) * 2010-12-04 2012-01-11 赵敏 Fresh pomegranate probiotic beverage and preparation method thereof
CN105167036A (en) * 2015-06-09 2015-12-23 青岛博恩高科生物技术有限公司 Pomegranate juice beverage and preparation method thereof
CN105558731A (en) * 2016-01-15 2016-05-11 福建绿泉食品有限公司 Fruit and vegetable juice based on multi-bacterium combined fermenting technology and preparing method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108925982A (en) * 2018-07-03 2018-12-04 淮北师范大学 A kind of preparation method of high anti-oxidation activity pomegranate ferment
CN113875919A (en) * 2021-10-27 2022-01-04 陕西师范大学 Fermented pomegranate juice with blood pressure reducing function and preparation method thereof

Similar Documents

Publication Publication Date Title
CN108094790A (en) A kind of fermented apple juice and preparation method thereof
CN108065163A (en) A kind of fermentation coconut milk and preparation method thereof
CN102524387B (en) Kefir fermented dairy product and preparation process thereof
CN102986874B (en) Kefir viable type sour milk beverage and production method thereof
US20160192682A1 (en) Formulation and process for making fermented probiotic food and beverage products
CN106509529A (en) Brown lactic acid bacteria beverage containing enzyme and preparation method thereof
CN102100252A (en) Fermented dairy product and preparation method and raw material composition thereof, lactobacillus plantarum application
CN102972522B (en) Probiotic milk and preparation method thereof
CN107873841B (en) Lactic acid bacteria beverage and production process thereof
US20200352202A1 (en) Plant-based non-dairy fermented base composition and methods of making and using the same
CN108125087A (en) A kind of fermentation mulberry juice and preparation method thereof
CN107960581A (en) A kind of fermented grape juice and preparation method thereof
CN107927170A (en) A kind of double fermentation milk-contained drinks and preparation method thereof
CN104757116B (en) A kind of pickles Yoghourt and preparation method thereof
US20050153017A1 (en) Process for the preparation of a vegetable yogurt
CN107897373A (en) A kind of double acidified milk flavor beverages and preparation method thereof
CN103168853B (en) Fermentation dairy product and preparation method thereof
CN104920605A (en) Hot-filled fermented milk and preparation method thereof
CN107125427A (en) Double fermented frozen drinks and preparation method thereof
CN104304462A (en) Fruit type fermented milk and preparation method thereof
CN108157712A (en) A kind of fermentation juice of my pomegranate and preparation method thereof
CN108056346A (en) A kind of fermentation mango juice and preparation method thereof
CN108142552A (en) A kind of acidified milk of double fermentations and preparation method thereof
CN108142553A (en) A kind of acidified milk of double fermentations and preparation method thereof
CN104542978B (en) A kind of bowl mandarin orange Yoghourt and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20180615

RJ01 Rejection of invention patent application after publication