CN108902210A - A kind of safe and non-toxic essential oil for preventing and treating Bradysia fungus gnat larva - Google Patents
A kind of safe and non-toxic essential oil for preventing and treating Bradysia fungus gnat larva Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a kind of safe and non-toxic essential oils for preventing and treating Bradysia fungus gnat larva, are related to Biocidal field, it is characterised in that raw material is plurality of Chinese.Compared with the prior art, the composition of prevention and treatment olaiphage larva in Chinese Chive of the invention is safe and efficient, promotes agricultural product high yield, realizes the safe and nontoxic of agricultural product.
Description
Technical field
The present invention is application for a patent for invention《A kind of composition for preventing and treating Bradysia fungus gnat larva》（Application number：
2015100378274）Divisional application.It is related to Biocidal field, and in particular to a kind of prevention and treatment olaiphage larva in Chinese Chive
Composition and preparation method thereof.
Background technique
Leek category Liliaceae herbaceos perennial is the vegetables that numerous citizen, common people enjoy a lot.And Bradysia fungus gnat
（Bradysia odoriphaga Yang et Zhang）, the Liliaceaes such as main harm leek, shallot, onion, spring onion, garlic
Vegetables also endanger lettuce, green vegetables, celery etc. once in a while, are one of primary pests of allium vegetables.Its larva endangers form：Spring,
The young stem rot that two season of autumn mainly causes leek is rotten, keeps fragrant-flowered garlic leaf withered and yellow and dead.Summer larva, which moves downward, to eat into bulb, severe one bulb
It rots, whole pier leek is dead.
The method for preventing and treating olaiphage larva in Chinese Chive at present is mainly the organic phosphorus equal chemical pesticides of A Wei bacterium element ﹑, but is deposited
In following defect：1. olaiphage larva in Chinese Chive is lived in underground, general pesticide is difficult to be killed；2. using methylamine
The high residues such as phosphorus, Azodrin, highly toxic organophosphorus pesticide are watered pouring root and kill bradysia odoriphaga, because a large amount of organophosphorus pesticides can be by Radix Folium Allii tuberosi
Portion, which absorbs, to be remained in leek body, so being not easy simply to be washed, is poisoned after just eating people.
Also occur some botanical pesticides currently on the market, main function mechanism is mostly to have in virose using some
Medicine is deployed, though being plant source, is not still detached from big poison, and there are hidden danger in production and sales, and influences fragrant-flowered garlic
The natural growth of dish, causes the decline and taste deterioration of leek nutritive value.
Summary of the invention
Technical assignment of the invention is to provide a kind of prevention and treatment olaiphage larva in Chinese Chive for the above the deficiencies in the prior art
Composition, leek can be promoted to grow on the basis of guaranteeing vermins-proof effect.
The present invention the technical solution to solve the technical problem is that：A kind of prevention and treatment olaiphage larva in Chinese Chive granule, it is special
Sign is that bulk pharmaceutical chemicals and weight ratio are：10 ~ 35 parts of fructus piperis longi, 15 ~ 65 parts of bitter pot Lu, 10 ~ 40 parts of lycopodium calvatum, cloves 30 ~ 80
Part, 20 ~ 75 parts of asarum, 20 ~ 90 parts of rattan fiber crops, 3 ~ 30 parts of fortune holly fern rhizome, 2 ~ 40 parts of Gui Ding, 5 ~ 40 parts of red halloysite, 2 ~ 25 parts of sea lettuce, dog
1 ~ 20 part of bone wood, 10 ~ 60 parts of Herba Lycopi, 2 ~ 8 parts of centella, red 10 ~ 40 parts of back ma, 73 ~ 20 parts of crab, 8 ~ 20 parts of lycopodium,
8 ~ 60 parts of winebibber's grass, 5 ~ 15 parts of baccate sedge herb, 6 ~ 25 parts of faint scent osmanthus；10 ~ 15 parts of cassiae nodosae,semen, sea is 40 ~ 70 parts alizarin, Semen Leonuri
29 ~ 50 parts, 20 ~ 45 parts of asparagus fern, 20 ~ 30 parts of mussel, 17 ~ 30 parts of needle sand；
Preparation method is：
（1）Fructus piperis longi, crab seven, fortune holly fern rhizome, bitter pot Lu, cloves, asarum, Gui Ding, faint scent osmanthus coarse powder are passed through into supercritical fluid CO2
Extraction obtains mixed essential oil, and the dregs of a decoction are spare；
（2）By lycopodium calvatum, rattan fiber crops, dog bone wood, Herba Lycopi, centella, red back ma, Jiu Xiancao, red halloysite, lycopodium, sea lettuce, mountain barnyard grass
It is small that the water that the dregs of a decoction in son, cassiae nodosae,semen, Hai Qian, Semen Leonuri, asparagus fern, mussel, needle sand and step 1 mix 3 times of amounts of addition impregnates 12
Shi Hou is decocted 2 times, and 1 hour every time, collecting decoction stood filtration, it is spare to obtain filtrate；
（3）By filtrate obtained in step 2,15% propylene glycol is added, mixes, 4 DEG C of refrigeration standings filter to get filtrate；
（4）The resulting mixed essential oil of step 1 is added in filtrate obtained in step 1, is uniformly mixed, 100 DEG C of circulation steam sterilizations
30 minutes, packaging.
Fructus piperis longi in prescription, the nearly mature or mature fruit cluster of the drying for being Piperaceae plant fructus piperis longi Piper longum L..
Bitter pot Lu is cucurbitaceous plant small gourd Lagenaria siceraria（Molina） Standl var.
microcarpa（Naud.）The fruit of Hara.
Lycopodium calvatum is the drying herb of lycopsid lycopod Lycopodium japonicum Thunb..
Cloves is the dry flower of plant clove of myrtaceae Eugenia caryophllata Thunb.
Asarum is aristolochiaceae plant Herba Asari Asarum heterotropoides Fr. Var.mandshuricum
(Max ~ im.) Kitag., Seoul asarum Asarum sieboldii Miq.var.seoulense Nakai or asarum sieboldii
The drying herb of Asaru ~ m sieboldii Miq..
Rattan fiber crops, Urticaceae Elatostematis E rattan of plant fiber crops Elatostema laevigatum（Blume） Hassk.
（Procris laevigata Blume）, with all herbal medicine.
Fortune holly fern rhizome is the rhizome of Dryopteridaceae plant mountainous region rhizome of cyrtomium.
Gui Ding, the young okra fruit for being lauraceae plant cortex cinnamomi Cinnamomum cassia Presl are real.
Red halloysite is silicates mineral halloysite race halloysite, the main [ Al4 containing hydrated aluminium silicate
（Si4O10）（OH）8.4H2O].
Sea lettuce is Ulvaceae plant sea lettuce Ulva lactuca L, U. pertusa Ulva pertusa Kjellm, sliver stone
The frond of water shield Ulva fasciata Delile.
Dog bone wood, for madder wort South Mountain flower Prismatomeris tetrandra (Roxb.) k. Schum.'s
Root.
Herba Lycopi is the stem of Lamiaceae plant lycopus lucidus Lycopus lucidus Tu rcz. var.hirtus Regel
Leaf.
Centella is umbelliferae centella Centella asiatica（L.）Urb. drying herb.
Red back ma is mussel section mountain waste of flax platymiscium red back mountain hemp rod Alchornea trewioides（Benth.）
Muell. ~ Arg leaf.
Crab seven, for Liliaceae Smilacina plant tube flower Smilacina japonica Smilacina henryi（Baker） Wang et
The rhizomes and root of Tang.
Lycopodium is lycopsid lycopod copodium japonicum Thunb., crowfoot lycopod Lycopodium
Centro ~ chinense Ching, Spora Lycopodii Palhinhaea cernua（L.）Franco et Vasc. and single fringe
The spore of lycopod Lycopodium cernuum L..
Winebibber grass, for sandalwood plants come into leaves Thesium chinese Thesium longifolium Turcaz., dew column Thesium chinese
Thesium himalense Royle, Thesium longifolium Hand.-Mazz Thesium longiflorum Hand. ~ Mazz. or Thesium refractum C. A. Meyer
The herb or root of Thesium refractum C.A.Mey..
Baccate sedge herb is Cyperaceae carex baccate sedge herb Carex baccans Nees seed.
Flower Sarcococca ruscifolia Stapf root is fanned for Buxaceae Sarcococca plant open country in faint scent osmanthus.
Cassiae nodosae,semen is the seed of leguminous plant chick-pea Cicer arietinum L.
Hai Qian is Sargassaceae plant sargassum Sargassum enerve C.Ag., Sargassum henslowianum Sargassum
Henslowianum C.Ag., sargassum thunbergii Sargassum thunbergii（Mert.）O.Kuntze or Sargassum horneri argassum
horneri（Turn.）C. the frond of Ag.
Semen Leonuri is the dry mature fruit of Lamiaceae plant motherwort Leonurus japonicus Houtt..
Asparagus fern is Liliaceae asparagus fern Asparagus setaceus（Kunth）Jessop [A. plumosus Baker]
Root tuber.
Mussel is Mollusca lamellibranchiata Mytilidae Trachyostracous mussel Mytilus crassitesta Lischke；Mussel
M. edulis L.；Perna viridis M.（Chloromya） smaragdinus Chemnitz；Flat top clam Modiolus
modiolus （L.）；Clam M. philippinarum Hanley pushes up partially in Philippine, is used as medicine with meat.
Needle sand, fines when for steel needle processed under mill, forges vinegar with fire and quenches, finely ground to be used as medicine.Main component is iron, and impurity is oxidation
Iron（Hyrate）Deng, but the also elements such as normal carbon containing, phosphorus, silicon, sulphur.
Compared with prior art, invention has beneficial effect following prominent：
1, the purpose of prescription of the present invention not " kills " olaiphage larva in Chinese Chive, but by the drug ingedient of said medicine into
Row is driven, and interferes the growing environment of olaiphage larva in Chinese Chive；
2, fructus piperis longi, crab seven, fortune holly fern rhizome, bitter pot Lu, cloves, asarum, Gui Ding, faint scent osmanthus mixed volatilization oil component can be effectively
Expel olaiphage larva in Chinese Chive；
3, the leek inoculation after lycopodium calvatum, rattan fiber crops, dog bone wood, Herba Lycopi, centella, red back ma, winebibber's grass mixing Aqueous extracts impregnate
Olaiphage larva in Chinese Chive, using sterile water as blank control, 5d corrected mortality 79.93%.
4, the active constituent Yi Bei Duo Huo Su contained in mussel（MSM）, MSM can reduce olaiphage larva in Chinese Chive secretion silk
Line ability；
5, lycopodium, baccate sedge herb, sea lettuce, cassiae nodosae,semen, Hai Qian, Semen Leonuri, asparagus fern, mussel facilitate leek growth, and red halloysite,
Needle sand provides minerals and metal ion；
6, easy to use, highly-safe, safety interval is short, and will not damage to ambient enviroment.
Specific embodiment
Below in conjunction with actual conditions, elaborate to a specific embodiment of the invention.
Embodiment 1
In embodiment 1, bulk pharmaceutical chemicals and weight ratio are：10 parts of fructus piperis longi, 15 parts of bitter pot Lu, 40 parts of lycopodium calvatum, cloves 30
Part, 20 parts of asarum, 20 parts of rattan fiber crops, 3 parts of fortune holly fern rhizome, 40 parts of Gui Ding, 5 parts of red halloysite, 2 parts of sea lettuce, 1 part of wood of dog bone, 10 parts of Herba Lycopi,
2 parts of centella, red 10 parts of back ma, 73 parts of crab, 8 parts of lycopodium, 8 parts of winebibber's grass, 15 parts of baccate sedge herb, 25 parts of faint scent osmanthus.
The preparation method of embodiment 1 is：
（1）Fructus piperis longi, crab seven, fortune holly fern rhizome, bitter pot Lu, cloves, asarum, Gui Ding, faint scent osmanthus coarse powder are extracted into volatile oil respectively, and
Mixing for standby use after collection, the dregs of a decoction are spare；
（2）By lycopodium calvatum, rattan fiber crops, dog bone wood, Herba Lycopi, centella, red back ma, Jiu Xiancao, red halloysite, lycopodium, sea lettuce, mountain barnyard grass
The dregs of a decoction in son and step 1 mix the water that 3 times of amounts are added, and decoct 2 times, 1 hour every time, collecting decoction stood filtration, obtains filtrate
It is spare；
（3）By filtrate obtained in step 2,30% emulsifier is added and microemulsion is made in 40% water；
（4）Volatile oil obtained in step 1 is sprayed into microemulsion obtained in step 2, is mixed.
Wherein, the method for volatile oil being extracted in step 1 can be steam distillation extraction method.
Percentage in the step 3 is calculated with filtrate weight, and emulsifier used is anhydrous sorbitol list oil
Acid esters（Sorbester p17）.
Embodiment 2
In example 2, bulk pharmaceutical chemicals and weight ratio are：35 parts of fructus piperis longi, 65 parts of bitter pot Lu, 10 parts of lycopodium calvatum, 80 parts of cloves,
75 parts of asarum, 90 parts of rattan fiber crops, 30 parts of fortune holly fern rhizome, 2 parts of Gui Ding, 40 parts of red halloysite, 25 parts of sea lettuce, 20 parts of wood of dog bone, Herba Lycopi
60 parts, 8 parts of centella, red 40 parts of back ma, 7 20 parts of crab, 20 parts of lycopodium, 60 parts of winebibber's grass, 5 parts of baccate sedge herb, faint scent osmanthus 6
Part.
The preparation method of embodiment 2 is：
（1）It is packed into diafiltration kettle after above-mentioned raw materials are crushed, adds the ethyl alcohol that 3 times of amount volumetric concentrations are 99%, is heated to 50 DEG C, heating 6
Hour, obtain percolate and filter residue；
（2）The percolate that step 1 is obtained injects distillation still, distills to obtain distillate；
（3）After the filter residue dealcoholysis that step 1 is obtained, 3 times of amount water is added to decoct 1 hour, filtering, filtrate is concentrated into relative density 1.36
（60℃）；
（4）It after the concentration filtrate spray drying that step 3 is obtained, the humic acid of weight such as is mixed into, and sprays into distillation obtained by step 1
Liquid, drying and granulating is at granule.
Embodiment 3
In embodiment 3, bulk pharmaceutical chemicals and weight ratio are：22 parts of fructus piperis longi, 45 parts of bitter pot Lu, 20 parts of lycopodium calvatum, 55 parts of cloves,
40 parts of asarum, 55 parts of rattan fiber crops, 16 parts of fortune holly fern rhizome, 21 parts of Gui Ding, 22 parts of red halloysite, 13 parts of sea lettuce, 10 parts of wood of dog bone, Herba Lycopi 35
Part, 5 parts of centella, red 25 parts of back ma, 7 11 parts of crab, 14 parts of lycopodium, 34 parts of winebibber's grass, 10 parts of baccate sedge herb, faint scent osmanthus 15
Part.
The preparation method of embodiment 3：
（1）Fructus piperis longi, crab seven, fortune holly fern rhizome, bitter pot Lu, cloves, asarum, Gui Ding, faint scent osmanthus coarse powder are passed through into supercritical fluid CO2
Extraction obtains mixed essential oil, and the dregs of a decoction are spare；
（2）By lycopodium calvatum, rattan fiber crops, dog bone wood, Herba Lycopi, centella, red back ma, Jiu Xiancao, red halloysite, lycopodium, sea lettuce, mountain barnyard grass
The dregs of a decoction in son and step 1 mix the water that 3 times of amounts are added, and decoct 2 times, 1 hour every time, collecting decoction stood filtration, obtains filtrate
It is spare；
（3）By filtrate obtained in step 2,10% propylene glycol is added, mixes, refrigeration stands and filters to get filtrate；
（4）The resulting mixed essential oil of step 1 is added in filtrate obtained in step 3, is uniformly mixed, 100 DEG C of circulation steam sterilizations
30 minutes, packaging.
Percentage in the step 3 is calculated with filtrate weight.
Embodiment 4
In example 4, bulk pharmaceutical chemicals and weight ratio are：22 parts of fructus piperis longi, 45 parts of bitter pot Lu, 20 parts of lycopodium calvatum, 55 parts of cloves,
40 parts of asarum, 55 parts of rattan fiber crops, 16 parts of fortune holly fern rhizome, 21 parts of Gui Ding, 22 parts of red halloysite, 13 parts of sea lettuce, 10 parts of wood of dog bone, Herba Lycopi 35
Part, 5 parts of centella, red 25 parts of back ma, 7 11 parts of crab, 14 parts of lycopodium, 34 parts of winebibber's grass, 10 parts of baccate sedge herb, faint scent osmanthus 15
Part, 5 parts of cassiae nodosae,semen, sea are 10 parts, 8 parts of Semen Leonuri, 30 parts of asparagus fern, 10 parts of mussel, 5 parts of needle sand alizarin.
The preparation method of embodiment 4：
（1）Fructus piperis longi, crab seven, fortune holly fern rhizome, bitter pot Lu, cloves, asarum, Gui Ding, faint scent osmanthus coarse powder are extracted into volatile oil respectively, and
Mixing for standby use after collection, the dregs of a decoction are spare；
（2）By lycopodium calvatum, rattan fiber crops, dog bone wood, Herba Lycopi, centella, red back ma, Jiu Xiancao, red halloysite, lycopodium, sea lettuce, mountain barnyard grass
The dregs of a decoction in son, cassiae nodosae,semen, Hai Qian, Semen Leonuri, asparagus fern, mussel, needle sand and step 1 mix the filtrate being added in step 2, decoct
2 times, 1 hour every time, collecting decoction stood filtration, it is spare to obtain filtrate；
（3）By filtrate obtained in step 2,40% emulsifier is added and microemulsion is made in 30% water；
（4）Volatile oil obtained in step 1 is sprayed into microemulsion obtained in step 2, is mixed.
Wherein, the method for volatile oil being extracted in step 1 can be steam distillation extraction method.
Percentage in the step 3 is calculated with filtrate weight.Emulsifier used is polyoxyethylene sorbitan mountain
Pears alcohol monostearate（Polysorbate60）.
Embodiment 5
In embodiment 5, bulk pharmaceutical chemicals and weight ratio are：10 parts of fructus piperis longi, 15 parts of bitter pot Lu, 40 parts of lycopodium calvatum, cloves 30
Part, 20 parts of asarum, 20 parts of rattan fiber crops, 3 parts of fortune holly fern rhizome, 40 parts of Gui Ding, 5 parts of red halloysite, 2 parts of sea lettuce, 1 part of wood of dog bone, 10 parts of Herba Lycopi,
2 parts of centella, red 10 parts of back ma, 73 parts of crab, 8 parts of lycopodium, 8 parts of winebibber's grass, 15 parts of baccate sedge herb, 25 parts of faint scent osmanthus, the Huis
15 parts of beans, sea is 70 parts, 50 parts of Semen Leonuri, 20 parts of asparagus fern, 30 parts of mussel, 30 parts of needle sand alizarin.
The preparation method of embodiment 5 is：
（1）It is packed into diafiltration kettle after above-mentioned raw materials are crushed, adds the ethyl alcohol that 4 times of amount volumetric concentrations are 98%, is heated to 45 DEG C, heating
10 hours, obtain percolate and filter residue；
（2）The percolate that step 1 is obtained injects distillation still, distills to obtain distillate；
（3）After the filter residue dealcoholysis that step 1 is obtained, 4 times of amount water are added to decoct 2 times, 1 hour every time, filtering, filtrate was concentrated after merging
To relative density 1.34（60℃）；
（4）It after the concentration filtrate spray drying that step 3 is obtained, the bentonite of weight such as is mixed into, and sprays into distillation obtained by step 1
Granule is made in liquid, granulator.
Embodiment 6
In embodiment 6, bulk pharmaceutical chemicals and weight ratio are：35 parts of fructus piperis longi, 65 parts of bitter pot Lu, 10 parts of lycopodium calvatum, 80 parts of cloves,
75 parts of asarum, 90 parts of rattan fiber crops, 30 parts of fortune holly fern rhizome, 2 parts of Gui Ding, 40 parts of red halloysite, 25 parts of sea lettuce, 20 parts of wood of dog bone, Herba Lycopi
60 parts, 8 parts of centella, red 40 parts of back ma, 7 20 parts of crab, 20 parts of lycopodium, 60 parts of winebibber's grass, 5 parts of baccate sedge herb, faint scent osmanthus 6
Part, 10 parts of cassiae nodosae,semen, sea is 40 parts, 29 parts of Semen Leonuri, 45 parts of asparagus fern, 20 parts of mussel, 17 parts of needle sand alizarin.
The preparation method of embodiment 6 is：
（1）Fructus piperis longi, crab seven, fortune holly fern rhizome, bitter pot Lu, cloves, asarum, Gui Ding, faint scent osmanthus coarse powder are passed through into supercritical fluid CO2
Extraction obtains mixed essential oil, and the dregs of a decoction are spare；
（2）By lycopodium calvatum, rattan fiber crops, dog bone wood, Herba Lycopi, centella, red back ma, Jiu Xiancao, red halloysite, lycopodium, sea lettuce, mountain barnyard grass
It is small that the water that the dregs of a decoction in son, cassiae nodosae,semen, Hai Qian, Semen Leonuri, asparagus fern, mussel, needle sand and step 1 mix 3 times of amounts of addition impregnates 12
Shi Hou is decocted 2 times, and 1 hour every time, collecting decoction stood filtration, it is spare to obtain filtrate；
（3）By filtrate obtained in step 2,15% propylene glycol is added, mixes, 4 DEG C of refrigeration standings filter to get filtrate；
（4）The resulting mixed essential oil of step 1 is added in filtrate obtained in step 1, is uniformly mixed, 100 DEG C of circulation steam sterilizations
30 minutes, packaging.
Percentage in the step 3 is calculated with filtrate weight.
Diafiltration, distillation in preparation process described in embodiment 1 ~ 6, circulation diafiltration, supercritical fluid CO2 extraction, dealcoholysis,
Filtrate concentration, microemulsion preparation, granule preparation, steam sterilization, can using technique in the prior art realization, not by
Technique, equipment are limited.
Mentioned component efficient combination fast and effectively completes the task of desinsection, and production cost is low, using plant material,
Chun Ran ﹑ are nuisanceless, environmentally protective, and the above results are that experimental data sufficiently proves that relevant information is as follows.
One, desk research
1, object and method
1.1 object：
1.1.1 potting：A collection of potting is cultivated in advance without insect pest leek, flowerpot diameter 20cm.
1.1.2 test worm：The larva that Bradysia odoriphaga is acquired from the leek planting site that east saves Liaocheng City, is placed in and is covered with filter
In the glass culture dish of paper moisturizing, feed is done with the red kind vacation stem portion of the leek only son and heir, is continuously raised in (25 ± 1) DEG C insulating box
It supports.Clean culture dish is replaced in time, and timing adding water keeps suitable humidity.
1.1.3 tested medicament and grouping：
1. chlopyrifos group：Chlopyrifos（48% Le Siben missible oil）, The Dow Agrosciences, LLC. dilutes 1000 times, and dosage is
100ml/ mus.
2. testing 1 group：1 gained pesticide of embodiment, dosage 1.34kg（Crude drug）/ mu.
3. testing 2 groups：2 gained pesticide of embodiment, dosage 3.46kg（Crude drug）/ mu.
4. testing 3 groups：3 gained pesticide of embodiment, dosage 2.34kg（Crude drug）/ mu.
5. testing 4 groups：4 gained pesticide of embodiment, dosage 2.68kg（Crude drug）/ mu.
6. testing 5 groups：5 gained pesticide of embodiment, dosage 2.41kg（Crude drug）/ mu.
7. testing 6 groups：6 gained pesticide of embodiment, dosage 4.26kg（Crude drug）/ mu.
8. model group：Clear water.
9. blank group：Clear water.
1.2 method：
1.2.1 potting pharmacodynamic test：In topsoil at leek vacation stem, in addition to blank group, every basin connects 4 age of bradysia odoriphaga of 3 ages in days
Larva 20, then stablize makes it pierce in the soil of leek basal part of stem for 24 hours is poured with the medical fluid diluted, only to pour clear water
Conduct control.The survival of inspection in 5th day borer population after processing.Calculate each group death rate and corrected mortality.
1.2.2 plant evaluation in the 5th day after the processing of each group leek.
2, result
1.2.1 potting pharmacodynamic test result：It see the table below,
| Grouping | The death rate（%） | Correct the death rate（%） |
| Chlopyrifos group | 100 | 100 |
| Test 1 group | 82.6 | 81.9 |
| Test 2 groups | 91.2 | 88.4 |
| Test 3 groups | 87.1 | 86.4 |
| Test 4 groups | 89.3 | 87.3 |
| Test 5 groups | 83.7 | 82.9 |
| Test 6 groups | 90.5 | 88.5 |
| Model group | 12.8 | - |
| Blank group | - | - |
It can thus be seen that killing effect is extremely bright using 1 ~ 6 group of experiment of 1 ~ 6 gained pesticide of embodiment compared with model group
It is aobvious.
1.2.2 plant evaluation in the 5th day after the processing of each group leek：It is control, model group leek plant height, leaf with blank group
Tablet quality is decreased obviously, and 1 ~ 3 group of chlopyrifos group and experiment are compared with blank group, no significant difference.And it is high to test 4 ~ 6 groups of plant
Degree, leaf quality are better than blank group.
Two, field is studied
1, materials and methods
1.1.1 tested vegetables and region：Select the leek planting site of Liaocheng of Shandong Province.
1.1.2 tested medicament and grouping：
1. chlopyrifos group：Chlopyrifos（48% Le Siben missible oil）, The Dow Agrosciences, LLC. dilutes 1000 times, and dosage is
100ml/ mus.
②：1 gained pesticide of embodiment, dosage 1.34kg（Crude drug）/ mu.
3. testing 2 groups：2 gained pesticide of embodiment, dosage 3.46kg（Crude drug）/ mu.
4. testing 3 groups：3 gained pesticide of embodiment, dosage 2.34kg（Crude drug）/ mu.
5. testing 4 groups：4 gained pesticide of embodiment, dosage 2.68kg（Crude drug）/ mu.
6. testing 5 groups：5 gained pesticide of embodiment, dosage 2.41kg（Crude drug）/ mu.
7. testing 6 groups：6 gained pesticide of embodiment, dosage 4.26kg（Crude drug）/ mu.
8. blank group：Clear water.
1.2 method：The application method of each experimental group is to pour leek kind wherein before early spring leek germinates after diluting
It plants in ground, interval time needed for measurement leek stem drug residue drops to maximum allowable residual quantity after application every time.
2, result
2.1 60d each groups prevent and treat effective percentage：Chlopyrifos group 99%, experiment 1 group 88%, experiment 2 group 96%, experiment 3 group 95%,
4 group 94%, 5 group 91% of experiment, 6 group 98% of experiment are tested, compared with blank group, there are notable differences.Each experimental group and chlopyrifos group
Between, no significant difference（P>0.05）.
2.2 safety interval：It is repeatedly measured, the chlopyrifos residue amount of leek stem drops to maximum allowable residual quantity institute
Taking time is 6.8 days, and 12h measurement has no toxic residual after the present invention tests 1 ~ 6 group of medication, it is therefore seen that the peace of prescription of the present invention
Full interval is significantly shorter than chlopyrifos.
By the studies above the results show that the insecticide desinsection using prescription of the present invention is efficient high, killing ability and
Chlopyrifos is similar, but safety interval is shorter, and nontoxic, highly-safe, will not damage to ambient enviroment.
It should be noted that the specific embodiment of invention is described in detail invention, for this field
For technical staff, all exist without departing substantially from the spirit and scope of invention to the various obvious changes that it is carried out
Within the protection scope of invention.
Claims (1)
1. a kind of safe and non-toxic essential oil for preventing and treating Bradysia fungus gnat larva, which is characterized in that bulk pharmaceutical chemicals and weight ratio are：Fructus piperis longi 10
~ 35 parts, 15 ~ 65 parts of bitter pot Lu, 10 ~ 40 parts of lycopodium calvatum, 30 ~ 80 parts of cloves, 20 ~ 75 parts of asarum, 20 ~ 90 parts of rattan fiber crops, fortune holly fern rhizome 3
~ 30 parts, 2 ~ 40 parts of Gui Ding, 5 ~ 40 parts of red halloysite, 2 ~ 25 parts of sea lettuce, 1 ~ 20 part of dog bone wood, 10 ~ 60 parts of Herba Lycopi, centella 2 ~ 8
Part, red 10 ~ 40 parts of back ma, 73 ~ 20 parts of crab, 8 ~ 20 parts of lycopodium, 8 ~ 60 parts of winebibber's grass, 5 ~ 15 parts of baccate sedge herb, clearly
6 ~ 25 parts of fragrant osmanthus；10 ~ 15 parts of cassiae nodosae,semen, sea is 40 ~ 70 parts alizarin, and 29 ~ 50 parts of Semen Leonuri, 20 ~ 45 parts of asparagus fern, 20 ~ 30 parts of mussel,
17 ~ 30 parts of needle sand；
Preparation method is：
（1）Fructus piperis longi, crab seven, fortune holly fern rhizome, bitter pot Lu, cloves, asarum, Gui Ding, faint scent osmanthus coarse powder are passed through into supercritical fluid CO2
Extraction obtains mixed essential oil, and the dregs of a decoction are spare；
（2）By lycopodium calvatum, rattan fiber crops, dog bone wood, Herba Lycopi, centella, red back ma, Jiu Xiancao, red halloysite, lycopodium, sea lettuce, mountain barnyard grass
It is small that the water that the dregs of a decoction in son, cassiae nodosae,semen, Hai Qian, Semen Leonuri, asparagus fern, mussel, needle sand and step 1 mix 3 times of amounts of addition impregnates 12
Shi Hou is decocted 2 times, and 1 hour every time, collecting decoction stood filtration, it is spare to obtain filtrate；
（3）By filtrate obtained in step 2,15% propylene glycol is added, mixes, 4 DEG C of refrigeration standings filter to get filtrate；
（4）The resulting mixed essential oil of step 1 is added in filtrate obtained in step 1, is uniformly mixed, 100 DEG C of circulation steam sterilizations
30 minutes, packaging.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810678828.0A CN108902210A (en) | 2015-01-26 | 2015-01-26 | A kind of safe and non-toxic essential oil for preventing and treating Bradysia fungus gnat larva |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510037827.4A CN104604962B (en) | 2015-01-26 | 2015-01-26 | A kind of composition of prevention Bradysia fungus gnat larva |
| CN201810678828.0A CN108902210A (en) | 2015-01-26 | 2015-01-26 | A kind of safe and non-toxic essential oil for preventing and treating Bradysia fungus gnat larva |
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| CN201510037827.4A Division CN104604962B (en) | 2015-01-26 | 2015-01-26 | A kind of composition of prevention Bradysia fungus gnat larva |
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| CN201810678828.0A Pending CN108902210A (en) | 2015-01-26 | 2015-01-26 | A kind of safe and non-toxic essential oil for preventing and treating Bradysia fungus gnat larva |
| CN201810681564.4A Pending CN109042746A (en) | 2015-01-26 | 2015-01-26 | A kind of plants essential oil pesticide for preventing and treating Bradysia fungus gnat larva |
| CN201510037827.4A Active CN104604962B (en) | 2015-01-26 | 2015-01-26 | A kind of composition of prevention Bradysia fungus gnat larva |
| CN201810681565.9A Pending CN108935543A (en) | 2015-01-26 | 2015-01-26 | A kind of prevention and treatment Bradysia fungus gnat larva granule |
| CN201810678829.5A Pending CN108887300A (en) | 2015-01-26 | 2015-01-26 | A kind of nontoxic granule for preventing and treating olaiphage larva in Chinese Chive |
| CN201810681561.0A Pending CN109105402A (en) | 2015-01-26 | 2015-01-26 | A kind of microemulsion for preventing and treating Bradysia fungus gnat larva |
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| CN201810681564.4A Pending CN109042746A (en) | 2015-01-26 | 2015-01-26 | A kind of plants essential oil pesticide for preventing and treating Bradysia fungus gnat larva |
| CN201510037827.4A Active CN104604962B (en) | 2015-01-26 | 2015-01-26 | A kind of composition of prevention Bradysia fungus gnat larva |
| CN201810681565.9A Pending CN108935543A (en) | 2015-01-26 | 2015-01-26 | A kind of prevention and treatment Bradysia fungus gnat larva granule |
| CN201810678829.5A Pending CN108887300A (en) | 2015-01-26 | 2015-01-26 | A kind of nontoxic granule for preventing and treating olaiphage larva in Chinese Chive |
| CN201810681561.0A Pending CN109105402A (en) | 2015-01-26 | 2015-01-26 | A kind of microemulsion for preventing and treating Bradysia fungus gnat larva |
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| CN105145488B (en) * | 2015-07-08 | 2019-09-06 | 山东省农业科学院植物保护研究所 | A method of it traps and monitors bradysia odoriphaga larva |
| CN106035409A (en) * | 2016-06-03 | 2016-10-26 | 重庆市璧山区两座种养殖农民专业合作社 | Composition for killing Chinese chive maggots |
| CN107094812A (en) * | 2017-03-09 | 2017-08-29 | 海门市嘉富来农产品有限公司 | A kind of fruit tree insecticide and preparation method thereof |
| PL235769B1 (en) * | 2017-06-20 | 2020-10-19 | Soltys Jozef Przed Techniczno Handlowe Intermark | Preparation for fighting insects and method for applying it |
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| CN102742611A (en) * | 2012-06-21 | 2012-10-24 | 毛松 | Chinese herbal medicine prescription for preventing and curing Chinese chive maggot |
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| CN1332613C (en) * | 2006-05-29 | 2007-08-22 | 李玉珠 | Plant-type pesticide |
| CN102948433A (en) * | 2011-08-25 | 2013-03-06 | 天津益博生物技术开发有限公司 | Traditional Chinese medicine pesticide |
| CN102578171B (en) * | 2012-01-11 | 2013-09-18 | 张彦斌 | Chinese herbal medicinal insecticide |
| CN102784350A (en) * | 2012-08-07 | 2012-11-21 | 孙雅儒 | Mite killing bactericide and preparation method of mite killing bactericide |
| CN103598219A (en) * | 2013-10-28 | 2014-02-26 | 柯义清 | Method for biologically preventing bradysia odoriphaga |
| CN103931678B (en) * | 2014-04-23 | 2015-11-18 | 山东省农业科学院植物保护研究所 | A kind of biopesticide and preparation technology thereof preventing and treating Bradysia fungus gnat |
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| CN102742611A (en) * | 2012-06-21 | 2012-10-24 | 毛松 | Chinese herbal medicine prescription for preventing and curing Chinese chive maggot |
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| Title |
|---|
| 李敏，郭力: "《中药学专业知识 二》", 30 April 2009, 中国医药科技出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108887300A (en) | 2018-11-27 |
| CN104604962B (en) | 2018-08-28 |
| CN104604962A (en) | 2015-05-13 |
| CN108935543A (en) | 2018-12-07 |
| CN109105402A (en) | 2019-01-01 |
| CN109042746A (en) | 2018-12-21 |
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Application publication date: 20181130 |