CN108902207A - A kind of human papilloma virus inactivator and preparation method thereof - Google Patents
A kind of human papilloma virus inactivator and preparation method thereof Download PDFInfo
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- CN108902207A CN108902207A CN201810564550.4A CN201810564550A CN108902207A CN 108902207 A CN108902207 A CN 108902207A CN 201810564550 A CN201810564550 A CN 201810564550A CN 108902207 A CN108902207 A CN 108902207A
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- papilloma virus
- human papilloma
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- 241000701806 Human papillomavirus Species 0.000 title claims abstract description 41
- 230000000937 inactivator Effects 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims abstract description 29
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000004471 Glycine Substances 0.000 claims abstract description 14
- 229960005323 phenoxyethanol Drugs 0.000 claims abstract description 14
- 235000002732 Allium cepa var. cepa Nutrition 0.000 claims abstract description 9
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract description 9
- 244000246386 Mentha pulegium Species 0.000 claims abstract description 9
- 235000016257 Mentha pulegium Nutrition 0.000 claims abstract description 9
- 235000004357 Mentha x piperita Nutrition 0.000 claims abstract description 9
- 239000010231 banlangen Substances 0.000 claims abstract description 9
- 235000001050 hortel pimenta Nutrition 0.000 claims abstract description 9
- 241001116389 Aloe Species 0.000 claims abstract description 7
- 235000011399 aloe vera Nutrition 0.000 claims abstract description 7
- VOPQXYCIRQXBGE-UHFFFAOYSA-M dimethyl-bis(sulfanyl)azanium chloride Chemical compound [Cl-].S[N+](C)(C)S VOPQXYCIRQXBGE-UHFFFAOYSA-M 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 9
- 241000234282 Allium Species 0.000 claims description 8
- 239000000287 crude extract Substances 0.000 claims description 8
- 229920001661 Chitosan Polymers 0.000 claims description 7
- 239000008363 phosphate buffer Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- 239000002002 slurry Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 4
- 230000006196 deacetylation Effects 0.000 claims description 3
- 238000003381 deacetylation reaction Methods 0.000 claims description 3
- 238000005189 flocculation Methods 0.000 claims description 3
- 230000016615 flocculation Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 235000014676 Phragmites communis Nutrition 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 241001631646 Papillomaviridae Species 0.000 claims 1
- 239000004744 fabric Substances 0.000 claims 1
- 230000000415 inactivating effect Effects 0.000 abstract description 4
- 230000002421 anti-septic effect Effects 0.000 abstract description 3
- 239000000419 plant extract Substances 0.000 abstract description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 abstract 2
- 244000291564 Allium cepa Species 0.000 abstract 1
- 235000019270 ammonium chloride Nutrition 0.000 abstract 1
- 230000002779 inactivation Effects 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 208000009608 Papillomavirus Infections Diseases 0.000 description 2
- 239000012930 cell culture fluid Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 208000021145 human papilloma virus infection Diseases 0.000 description 2
- 201000010153 skin papilloma Diseases 0.000 description 2
- 206010059313 Anogenital warts Diseases 0.000 description 1
- 208000007860 Anus Neoplasms Diseases 0.000 description 1
- 208000000907 Condylomata Acuminata Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 208000000260 Warts Diseases 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 201000011165 anus cancer Diseases 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 208000019065 cervical carcinoma Diseases 0.000 description 1
- 201000004196 common wart Diseases 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 208000003154 papilloma Diseases 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002477 vacuolizing effect Effects 0.000 description 1
Classifications
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
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- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
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- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
- A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
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Abstract
The invention belongs to biological antiseptic and field of medical technology, it is related to a kind of human papilloma virus inactivator and preparation method thereof.The human papilloma virus inactivator is made of following components according to the mass fraction：Dimercaptodimethyl ammonium chloride 0.5-3 parts by weight；Glycine 0.5-3 parts by weight；Phenoxyethanol 3-8 parts by weight；Plant component extracting solution 0.5-3 parts by weight；With water 83-95.5 parts by weight；Wherein, the plant component extracting solution includes the component extracting solution of following plant：Honeysuckle, Radix Isatidis, onion, aloe and peppermint.Human papilloma virus inactivator of the invention has a preferable inactivating efficacy to human papilloma virus, and containing plant extract component, it is at low cost, have a wide range of application.
Description
Technical field
The invention belongs to biological antiseptic and field of medical technology, inactivate more particularly, to a kind of human papilloma virus
Agent and preparation method thereof.
Background technique
Human papilloma virus is a kind of papilloma vacuolating virus A category for belonging to papovaviridae, is spherical DNA virus,
The scaly epithelium of human skin mucous membrane can be caused to be proliferated.Show as the symptoms such as verruca vulgaris, genital wart.With sharp wet in venereal disease
The disease incidence of wart rapidly rises and cervical carcinoma, cancer of anus etc. increase, and human papilloma virus infection increasingly causes the pass of people
Note.
Existing disinfectant and bactericidal agent have the antibacterial effect of wide spectrum mostly, specifically for a certain virus inactivator compared with
It is few, therefore, it is badly in need of a kind of human papilloma virus inactivator in biological antiseptic and field of medical technology.
Summary of the invention
The object of the present invention is to provide a kind of human papilloma virus inactivators and preparation method thereof.The human papilloma virus
Inactivator has a preferable inactivating efficacy to human papilloma virus, and containing plant extract component, it is at low cost, have a wide range of application.
To achieve the goals above, the present invention provides a kind of human papilloma virus inactivator, human papilloma virus inactivation
Agent is made of following components according to the mass fraction：
Wherein, the plant component extracting solution includes the component extracting solution of following plant：Honeysuckle, Radix Isatidis, onion, reed
Luxuriant growth and peppermint.
In accordance with the present invention it is preferred that the content of the dimercaptodimethyl ammonium chloride is 1~2 parts by weight, the glycine
Content be 1~2 parts by weight, the content of the Phenoxyethanol is 4~5 parts by weight, and the content of the plant component extracting solution is 1
~2 parts by weight, the content of the water are 89~93 parts by weight.
In the present invention, honeysuckle, Radix Isatidis, onion, aloe and peppermint mass ratio be preferably 1：0.8-1.2：0.8-
1.2：0.8-1.2：1.5-2.5, according to a kind of specific embodiment, honeysuckle, Radix Isatidis, onion, aloe and peppermint quality
Than being 1：1：1：1：2.
According to the present invention, above-mentioned plant component extracting solution can be obtained by various modes, according to the present invention a kind of preferred reality
Mode is applied, the plant component extracting solution is made by following steps：
A) honeysuckle, Radix Isatidis, onion, aloe and peppermint are ground or is crushed with pulverizer, obtain slurry；
B) slurry is filtered, obtains crude extract；
C) crude extract is mixed with phosphate buffer, it is then poly- according to every liter of mixed liquor addition 500-1500mg shell
The standard addition chitosan of sugar flocculates；
D) liquid after flocculation is filtered separation, obtained transparency liquid is the plant component extracting solution.
The extracting solution of various plants can also individually be made, then extracting solution is mixed, obtain the plant component extracting solution.
In accordance with the present invention it is preferred that the average molecular weight of the chitosan is 3 × 105Da~6 × 105Da, deacetylation
For 80-90%.
In accordance with the present invention it is preferred that the PH range of the phosphate buffer is 5.8~8.2.
In accordance with the present invention it is preferred that the additional amount of the phosphate buffer is 8-12 times of the crude extract.
In accordance with the present invention it is preferred that the filtering is carried out using 60~160 mesh filter clothes.
The second aspect of the present invention provides the preparation method of above-mentioned human papilloma virus inactivator, and this method includes：It will plant
Object component extracting solution, dimercaptodimethyl ammonium chloride, glycine, Phenoxyethanol and water mixing, obtain the human papilloma virus
Inactivator.Specifically, dimercaptodimethyl ammonium chloride is sequentially added into plant component extracting solution, glycine, Phenoxyethanol, so
After water is added.
Human papilloma virus inactivator of the invention has preferable inactivating efficacy to human papilloma virus, and contains plant extract
Ingredient, it is at low cost, have a wide range of application.
Other features and advantages of the present invention will then part of the detailed description can be specified.
Specific embodiment
The preferred embodiment of the present invention is described in more detail below.Although the following describe preferred implementations of the invention
Mode, however, it is to be appreciated that may be realized in various forms the present invention without that should be limited by the embodiments set forth herein.
Preparation example 1
Plant component extracting solution is prepared using following methods.
1) by honeysuckle, Radix Isatidis, onion, aloe, peppermint according to 1：1：1：1：2 ratio is ground or is broken with pulverizer
It is broken, obtain slurry；
2) slurry is filtered with 60~160 mesh filter clothes, obtains crude extract；
3) crude extract is mixed with 10 times of volume phosphate buffers (pH7.0), is then added according to every liter of mixed liquor
Adding 1000mg chitosan, (average molecular weight is 5.5 × 105Da, deacetylation 86%) standard addition chitosan wad a quilt with cotton
It is solidifying；
D) liquid after flocculation is filtered separation, obtained transparency liquid is the plant component extracting solution.
Embodiment 1
Dimercaptodimethyl ammonium chloride, glycine are sequentially added into plant component extracting solution, then Phenoxyethanol is added
Water prepares human papilloma virus inactivator S1.The weight percent that each component accounts for composition total weight is as follows：
Embodiment 2
Dimercaptodimethyl ammonium chloride, glycine are sequentially added into plant component extracting solution, then Phenoxyethanol is added
Water prepares human papilloma virus inactivator S2.The weight percent that each component accounts for composition total weight is as follows：
Embodiment 3
Dimercaptodimethyl ammonium chloride, glycine are sequentially added into plant component extracting solution, then Phenoxyethanol is added
Water prepares human papilloma virus inactivator S3.The weight percent that each component accounts for composition total weight is as follows：
Embodiment 4
Dimercaptodimethyl ammonium chloride, glycine are sequentially added into plant component extracting solution, then Phenoxyethanol is added
Water prepares human papilloma virus inactivator S4.The weight percent that each component accounts for composition total weight is as follows：
Embodiment 5
Dimercaptodimethyl ammonium chloride, glycine are sequentially added into plant component extracting solution, then Phenoxyethanol is added
Water prepares human papilloma virus inactivator S5.The weight percent that each component accounts for composition total weight is as follows：
Embodiment 6
Dimercaptodimethyl ammonium chloride, glycine are sequentially added into plant component extracting solution, then Phenoxyethanol is added
Water prepares human papilloma virus inactivator S6.The weight percent that each component accounts for composition total weight is as follows：
Embodiment 7
Dimercaptodimethyl ammonium chloride, glycine are sequentially added into plant component extracting solution, then Phenoxyethanol is added
Water prepares human papilloma virus inactivator S7.The weight percent that each component accounts for composition total weight is as follows：
Embodiment 8
Dimercaptodimethyl ammonium chloride, glycine are sequentially added into plant component extracting solution, then Phenoxyethanol is added
Water prepares human papilloma virus inactivator S8.The weight percent that each component accounts for composition total weight is as follows：
Test case 1
Different parts human papilloma virus inactivation experiments.
1, experimental subjects：The tissue by human papilloma virus infection of different parts is taken, respectively number 1~5.
2, experimental design：Use the human papilloma virus inactivator S1-S8 made from embodiment 1-8 as test group 1-8.
Control group 1：75% ethyl alcohol.Control group 2：Clear water.
3, experimental method：Number 1~5 tissue is inactivated with test group, control group 1 and control group 2 respectively,
After 30min, the tissue after inactivation is put into cell culture fluid and is cultivated, whether observation cell culture fluid is infected afterwards for 24 hours.
4, experimental result, as shown in table 1.
Table 1
By human papilloma virus inactivation experiments, by the above results it is known that human papilloma virus of the invention inactivates
Agent has good inactivating efficacy to human papilloma virus.
Various embodiments of the present invention are described above, above description is exemplary, and non-exclusive, and
It is not limited to disclosed each embodiment.Without departing from the scope and spirit of illustrated each embodiment, for this skill
Many modifications and changes are obvious for the those of ordinary skill in art field.
Claims (9)
1. a kind of human papilloma virus inactivator, which is characterized in that the human papilloma virus inactivator is by according to the mass fraction
Following components composition：
Wherein, the plant component extracting solution includes the component extracting solution of following plant：Honeysuckle, Radix Isatidis, onion, aloe and
Peppermint.
2. human papilloma virus inactivator according to claim 1, which is characterized in that the dimercaptodimethyl ammonium chloride
Content be 1~2 parts by weight, the content of the glycine is 1~2 parts by weight, and the content of the Phenoxyethanol is 4~5 weight
Part, the content of the plant component extracting solution is 1~2 parts by weight, and the content of the water is 89~93 parts by weight.
3. human papilloma virus inactivator according to claim 1, which is characterized in that honeysuckle, Radix Isatidis, onion, reed
The mass ratio of luxuriant growth and peppermint is 1：0.8-1.2：0.8-1.2：0.8-1.2：1.5-2.5.
4. human papilloma virus inactivator according to claim 1, which is characterized in that the plant component extracting solution passes through
Following steps are made：
A) honeysuckle, Radix Isatidis, onion, aloe and peppermint are ground or is crushed with pulverizer, obtain slurry；
B) slurry is filtered, obtains crude extract；
C) crude extract is mixed with phosphate buffer, then adds 500-1500mg chitosan according to every liter of mixed liquor
Standard addition chitosan flocculates；
D) liquid after flocculation is filtered separation, obtained transparency liquid is the plant component extracting solution.
5. human papilloma virus inactivator according to claim 4, which is characterized in that the average molecular weight of the chitosan
It is 3 × 105Da~6 × 105Da, deacetylation 80-90%.
6. human papilloma virus inactivator according to claim 4, which is characterized in that the PH range of the phosphate buffer
It is 5.8~8.2.
7. human papilloma virus inactivator according to claim 4, which is characterized in that the additional amount of the phosphate buffer
It is 8-12 times of the crude extract.
8. human papilloma virus inactivator according to claim 4, which is characterized in that the filtering utilizes 60~160 mesh
Filter cloth carries out.
9. the preparation method of human papilloma virus inactivator described in any one of claim 1-8, which is characterized in that the party
Method includes：By plant component extracting solution, dimercaptodimethyl ammonium chloride, glycine, Phenoxyethanol and water are mixed, and obtain the people
Papillomavirus inactivator.
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