CN108898394A - A kind of safflower DNA Rapid identification Internet of Things anti-fake traceability system and anti-fake source tracing method - Google Patents
A kind of safflower DNA Rapid identification Internet of Things anti-fake traceability system and anti-fake source tracing method Download PDFInfo
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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Abstract
The invention belongs to safflowers to identify anti-counterfeit field, and in particular to a kind of safflower DNA Rapid identification Internet of Things anti-fake traceability system and anti-fake source tracing method.The DNA chip and retrospect label used including cloud, DNA chip, identification apparatus, retrospect label, DNA searching terminal, retrospective retrival terminal safflower DNA Internet of Things anti-counterfeiting system, have being used for quickly detecting by DNA chip for biological detection experimental ability, and it is true and false by the identification of DNA big data analysis, it carries out anti-fake.Do not have being traced by tracing label for biological detection experimental ability, check safflower production, processing, storage, transaction, logistics, the information of terminal and detection, cloud can analyse whether to exist abnormal and analysis result is issued inquiry.By big data make trace tag queries to data as time and space change, even if copying identical retrospect label, abnormal request for information also can be by system discovery.It realizes the duplication for having efficiently controlled fake producer, forms preferably counterfeit protection measures.
Description
Technical field
The invention belongs to safflowers to identify anti-counterfeit field, and in particular to a kind of safflower DNA Rapid identification Internet of Things is anti-fake to trace to the source
System and anti-fake source tracing method.
Background technique
Safflower is the dry flower also known as red blue flower of composite family safflower plant safflower (Carthamus tinctorius L.),
Alias has safflower, thorn safflower, safflower dish etc..Safflower is China's traditional Chinese herbal medicine, there is good medical value.Safflower taste
Pungent, warm-natured, converge to heart and liver channels have activating microcirculation and removing stasis medicinal, removing blood stasis and acesodyne, blood pressure lowering and reducing blood lipid and other effects, in treatment coronary heart disease, height
There is certain curative effect in terms of blood pressure, cerebral hemorrhage disease.Safflower is recorded in version in 2015《Chinese Pharmacopoeia》In.Currently on the market
Safflower is many kinds of, and complicated component, TLC Identification be in current national Chinese materia medica preparation it is most common it is easy to operate,
Efficiently discrimination method has separation and identification capacity well, can effectively identify the authenticity of medicinal material, improves medicinal material
Quality.
Traditional is anti-fake mainly using physics modes such as anti-counterfeiting label, false proof card, anti-counterfeit packages, by the straight of assessor
Connect the subjective mode such as observation, touch, number inquiry.Therefore, as long as product is once dispatch from the factory, fake producer is gratifying to be intended to be subject to for ground
It copys, is not got rid of always by counterfeit destiny.
Summary of the invention
The present invention is in view of the above-mentioned problems, providing a kind of safflower DNA Rapid identification Internet of Things anti-fake traceability system and anti-fake tracing back
Source method.
The technical solution used in the present invention is as follows：A kind of safflower DNA Rapid identification Internet of Things anti-fake traceability system, including
Cloud, DNA chip, identification apparatus, retrospect label, DNA searching terminal, retrospective retrival terminal；
The cloud includes storage module, analysis module, cloud information receiving module, cloud message output module, described
Storage module stores safflower DNA database and safflower production, processing, storage, transaction, logistics, the information of terminal and detection,
The cloud information receiving module receives the data of DNA searching terminal and retrospective retrival terminal by network and sends data to
Analysis module, the analysis module analyze the received data of the information receiving module, related data are received from storage module, is right
Than analysis DNA data, by DNA analysis result data and analysis safflower production, processing, storage, transaction, logistics, terminal and inspection
Data and analysis result are simultaneously transmitted to message output module by the information of survey, and the cloud message output module transmits information to
DNA searching terminal, retrospective retrival terminal；
The DNA chip, including solid phase support object, gene probe；
Each retrospect label includes corresponding and unique id information, the id information, safflower production, processing, storage,
Transaction, logistics, the information of terminal and detection form the storage module in corresponding ID data packet storage beyond the clouds；
DNA searching terminal, including DNA data uploading module, DNA data reception module, the DNA data uploading module will
DNA chip detection data is transmitted to cloud, and the DNA data reception module receives cloud DNA analysis as a result, DNA searching terminal
It can be mobile phone, computer；
Retrospective retrival terminal includes retrospect label information read module, retrospective retrival data uploading module, retrospective retrival number
According to receiving module, the retrospect label information read module reads label information and label information is transmitted to retrospective retrival data
Label information and DNA testing result are transmitted to cloud, the retrospect inspection by uploading module, the retrospective retrival data uploading module
Rope data reception module receives safflower production, processing, storage, transaction, logistics, the information of terminal and detection.
The safflower DNA database is established in the following manner：
(1) certified products safflower sample is subjected to batch extracting；
(2) by the safflower DNA sample and DNA chip progress hybridization reaction after extraction；
It (3) will be in hybridization reaction result input database；
(4) a variety of safflower samples without brand, different size, different sources, different batches are passed sequentially through into step
(1), (2), (3) are detected, and form safflower DNA database.
In step (1), safflower sample is extracted by CTAB method, and detailed process is as follows：4ml CTAB is added in centrifuge tube
Extracting solution and 80ul mercaptoethanol preheat in 65 ± 5 DEG C of water-baths, take safflower sample, and liquid nitrogen grinding is transferred to preheating at powder
In centrifuge tube afterwards, sealing keeps the temperature 30 ± 10 minutes in 65 ± 5 DEG C of water-baths, taking-up centrifuge tube, fast cooling to room temperature,
4ml phenol: chloroform: 25: 24: 1 mixed solution of isoamyl alcohol is added, mixes, forms mixed emulsion；12000r/min centrifugation
10min takes supernatant, and the chloroform of 4ml: the mixed solution of isoamyl alcohol 24: 1 is added, in being placed at room temperature for 10- after mixing
20min, 12000r/min are centrifuged 10min, remove supernatant, and precipitating successively uses dehydrated alcohol, 70% ethanol washing, and TE buffering is added
The RNase of liquid and final concentration of 50 μ g/ml obtains safflower DNA sample in 37 DEG C of heat preservation 1h.
The gene probe includes following probe：
Probe one：GCGTGATATGCTTAAGTTCAG；
Probe two：AGTCCTACCTGATTTGAGGTC；
Probe three：TCTTTGAAGCATGAAC；
Probe four：CAGTAGGAGATAATTATC；
Probe five：TCGAGATAATTATCACAC；
Probe six：ATGAGCGTTCAAAGATTCGT.
It can also include existing known various plants DNA probe.
The DNA chip is surface plasma resonant sensor chip, and the solid phase support object is silicon chip, the solid phase
Layer of metal film is adhered on supporter surface, and the gene probe is fixed on metallic film surface.Utilize surface plasma resonance
(surface plasmon resonance, SPR) biosensor technology quickly detects the DNA of safflower.Surface plasma is total
Vibration (Surface Plasmon Resonance, SPR) is a kind of optical physics phenomenon.When a branch of P-polarized light is in certain angle
It is incident on prism end face in range, surface plasma-wave will be generated at the interface of prism and metallic film (Au or Ag).Work as incidence
When the propagation constant of light wave and the propagation constant of surface plasma-wave match, free electron in metal film is caused to generate resonance,
That is surface plasma resonance.When analysis, one layer of biomolecule identification film first is fixed in censorchip surface, then by sample to be tested
Chip surface is flowed through, if there is the molecule that can identify membrane interaction with the biomolecule of chip surface in sample, gold can be caused
Film surface variations in refractive index eventually leads to the variation of the angle SPR by detecting SPR angle change and obtains concentration, the parent of analyte
With the information such as power, kinetic constant and specificity.Occur both when safflower DNA sample after extraction is injected into chip surface special
The opposite sex combines, and so as to cause the variation of chip surface optical parameter, and shows as electronic signals, specific bond determinand
Amount it is different, the response intensity of signal is different.
The retrospect label is RFID RFID tag.Radio frequency identification, RFID (Radio Frequency
Identification) technology, also known as radio frequency identification are a kind of communication technologys, are commonly called as electronic tag.Radio can be passed through
Signal identification specific objective simultaneously reads and writes related data, connects without establishing mechanical or optics between identifying system and specific objective
Touching.Convenient for identification, make the information Rapid input of production, processing, storage, transaction, logistics, terminal and detection.Item can also be taken
Shape code, two dimensional code isotype, but need to be scanned one by one, it is unfavorable for the typing of high-volume information.
The retrospect label is arranged in DNA chip.Result can directly be will test after tester's detection to chase after by scanning
The label that traces back connects cloud, will test data and is transmitted to cloud, and obtains cloud analysis as a result, coherent detection data are saved in cloud
On end.
Agent, logistics, consumer need that proof of identity is registered and carried out on beyond the clouds, and the agent, logistics are to cloud
The data of correspondence ID in the storage module of end are surrounded by reading permission, number of the consumer to the correspondence ID in the storage module of cloud
It reads permission according to being surrounded by and forbids the user read permission in addition to the manager of cloud.Reading every time in this way has corresponding identity
Information produces exception reporting if the consumer of terminal repeatedly inquires, and informs the larger possible generation retrospect label of consumer
The case where being replicated, if there is consumer retracts commodity, sales end can typing correlation sales information, reduce the possibility of cloud erroneous judgement
Property.
After each ID data packet starts equipped with timing routine, self-generating ID on the cloud storage module after a certain period of time,
Retrospective retrival terminal can not retrieve.Cloud manager can be according to the characteristic of safflower product, and DNA is set according to actual conditions has
The effect phase avoids the retrospect label on expired product or expired product from shifting and uses.
A kind of anti-fake source tracing method of above-mentioned safflower DNA Rapid identification Internet of Things anti-fake traceability system, including it is following：
Personnel with detectability, detect in the following ways：
(1) DNA sample hybridize instead with DNA chip by the DNA sample that tested safflower sample is extracted by CTAB method
It answers, obtains response data；
(2) by retrospective retrival terminal scanning retrospect label, by response data by DNA searching terminal be uploaded to cloud and
Data in the DNA database of cloud compare, and obtain true and false result；
(3) it will test place, time, result are transmitted to cloud retrospect label safflower sample and correspond to ID number corresponding to batch
According to packet；
Do not have the personnel of detectability, identifies in the following ways：
(1) by retrospective retrival terminal scanning retrospect label, safflower production, processing, storage, transaction, logistics, terminal are obtained
With the information of detection；
(2) cloud carries out analysis according to historical query information and analysis result is sent to retrospective retrival terminal.
Beneficial effects of the present invention are as follows：The DNA chip and retrospect label that safflower DNA Internet of Things anti-counterfeiting system uses, tool
The Chinese medicine trading company of standby biological detection experimental ability, large-scale transaction place, hospital etc. can quickly be examined by DNA chip
It surveys, and true and false by the identification of DNA big data analysis, carries out anti-fake.Do not have pharmacy, the consumer etc. of biological detection experimental ability
It can be traced by tracing label, check safflower production, processing, storage, transaction, logistics, the information of terminal and detection, cloud
End can analyse whether to exist abnormal and analysis result is issued inquiry.By big data make to trace the data that arrive of tag queries with
Time and space and change, even if copying identical retrospect label, abnormal request for information also can be by system discovery.It realizes
The duplication of fake producer has been efficiently controlled, preferably counterfeit protection measures are formed.
Detailed description of the invention
Fig. 1 is the schematic diagram of safflower DNA Rapid identification Internet of Things anti-fake traceability system.
Fig. 2 is safflower DNA rapid identification method flow chart.
Specific embodiment
With reference to the accompanying drawing and specific embodiment, the present invention can be better described.
Embodiment one：
DNA chip is prepared, detailed process is as follows：
It can be Au or Ag 1. adhering to the metal film of one layer of 150nm by metal coating technique in silicon chip surface；
2. in probe genetic fragment sulfhydrylation, then by the probe points of different sulfhydrylations to different zones, in 4 DEG C of conditions
It is lower to be kept for 12 hours, wherein the gene probe includes following probe：
Probe one：GCGTGATATGCTTAAGTTCAG；
Probe two：AGTCCTACCTGATTTGAGGTC；
Probe three：TCTTTGAAGCATGAAC；
Probe four：CAGTAGGAGATAATTATC；
Probe five：TCGAGATAATTATCACAC；
Probe six：ATGAGCGTTCAAAGATTCGT；
3. then wash the unbonded probe of metallic film surface with deionized water, then with being dried with nitrogen.
There is good stability by DNA chip prepared by the above method, can be flowed for a long time on the market with product
Logical, the DNA probe on surface is designed for the DNA sequence dna of safflower, and some existing DNA of plants probes are being added, are passing through spy
Needle tests the DNA information of safflower sample, has specificity, good test effect.
Embodiment two：
Safflower DNA database is constructed, detailed process is as follows：
1. safflower sample is extracted by CTAB method, detailed process is as follows：In centrifuge tube be added 4ml CTAB extracting solution and
80ul mercaptoethanol preheats in 65 ± 5 DEG C of water-baths, takes safflower sample, centrifugation of the liquid nitrogen grinding at powder, after being transferred to preheating
Guan Zhong, sealing, keeps the temperature 30 ± 10 minutes in 65 ± 5 DEG C of water-baths, takes out centrifuge tube, and 4ml phenol is added in fast cooling to room temperature
: chloroform: 25: 24: 1 mixed solution of isoamyl alcohol mixes, and forms mixed emulsion；12000r/min is centrifuged 10min, takes
The chloroform of 4ml: the mixed solution of isoamyl alcohol 24: 1 is added in clear liquid, in being placed at room temperature for 10-20min after mixing,
12000r/min is centrifuged 10min, removes supernatant, and precipitating successively uses dehydrated alcohol, 70% ethanol washing, and TE buffer and end is added
Concentration is that the RNase of 50 μ g/ml obtains safflower DNA sample in 37 DEG C of heat preservation 1h.
2. by safflower DNA sample and DNA chip progress hybridization reaction after extraction：Safflower DNA sample is injected into DNA chip
Surface, hybridization reaction 5 hours at 45 DEG C.
3. the spr signal of each probe area is obtained, in input database；
4. a variety of safflower samples without brand, different size, different sources, different batches are passed sequentially through step 1 to 3
It is detected, forms safflower DNA database.
The DNA information in safflower sample is extracted by step 1, relatively other methods, extracting concentration is high, relatively complete
It is whole, hybridization reaction is carried out with DNA chip, reacts sensitiveer, conjugation is relatively high, observes the data in DNA database, discovery
Without brand, different size, different sources, different batches safflower sample data it is roughly the same, have part have differences.
Embodiment three：
Check the validity of DNA chip and safflower DNA database, testing staff does not know the true and false of tested safflower sample：
1. tested safflower sample is extracted by CTAB method, detailed process is as follows：4ml CTAB is added in centrifuge tube to mention
Liquid and 80ul mercaptoethanol are taken, is preheated in 65 ± 5 DEG C of water-baths, takes safflower sample, liquid nitrogen grinding is at powder, after being transferred to preheating
Centrifuge tube in, sealing keep the temperature 30 ± 10 minutes in 65 ± 5 DEG C of water-baths, and taking-up centrifuge tube, fast cooling to room temperature adds
Enter 4ml phenol: chloroform: 25: 24: 1 mixed solution of isoamyl alcohol, mix, forms mixed emulsion；12000r/min centrifugation
10min takes supernatant, and the chloroform of 4ml: the mixed solution of isoamyl alcohol 24: 1 is added, in being placed at room temperature for 10- after mixing
20min, 12000r/min are centrifuged 10min, remove supernatant, and precipitating successively uses dehydrated alcohol, 70% ethanol washing, and TE buffering is added
The RNase of liquid and final concentration of 50 μ g/ml obtains safflower DNA sample in 37 DEG C of heat preservation 1h.2. by the safflower DNA sample after extraction
Product and DNA chip carry out hybridization reaction：Safflower DNA sample is injected into DNA chip surface, hybridization reaction 5 hours at 45 DEG C.
3. obtaining the spr signal of each probe area, the spr signal data that will test are compared with safflower DNA database,
The sample that similarity is more than 90% is that very, the sample being less than is false.
4. a variety of safflower samples are passed sequentially through step 1 to detect to 3, the identification true and false is obtained as a result, detection accuracy
It is 100%.Illustrate that DNA chip and safflower DNA database can be effectively used for the identification of the safflower true and false.
Through this embodiment, it may be said that bright DNA chip and safflower DNA database can be effectively used for the identification of the safflower true and false.It is pseudo-
The DNA chip detection datas of pinkish red style product and safflower DNA database similarity are below 85%, and certified products safflower sample
DNA chip detection data and safflower DNA database similarity are above 95%, can obviously distinguish, pass through DNA chip in this way
Testing result error is small.It cloud can be between 85%-95% to the setting of certified products similarity limit.
Example IV：
A kind of safflower DNA Rapid identification Internet of Things anti-fake traceability system, including cloud, DNA chip, identification apparatus, retrospect
Label, DNA searching terminal, retrospective retrival terminal；
The cloud includes storage module, analysis module, cloud information receiving module, cloud message output module, described
Storage module stores safflower DNA database and safflower production, processing, storage, transaction, logistics, the information of terminal and detection,
The cloud information receiving module receives the data of DNA searching terminal and retrospective retrival terminal by network and sends data to
Analysis module, the analysis module analyze the received data of the information receiving module, related data are received from storage module, is right
Than analysis DNA data, by DNA analysis result data and analysis safflower production, processing, storage, transaction, logistics, terminal and inspection
Data and analysis result are simultaneously transmitted to message output module by the information of survey, and the cloud message output module transmits information to
DNA searching terminal, retrospective retrival terminal；
The DNA chip, including solid phase support object, gene probe；
Each retrospect label includes corresponding and unique id information, the id information, safflower production, processing, storage,
Transaction, logistics, the information of terminal and detection form the storage module in corresponding ID data packet storage beyond the clouds；
DNA searching terminal, including DNA data uploading module, DNA data reception module, the DNA data uploading module will
DNA chip detection data is transmitted to cloud, and the DNA data reception module receives cloud DNA analysis result；
Retrospective retrival terminal includes retrospect label information read module, retrospective retrival data uploading module, retrospective retrival number
According to receiving module, the retrospect label information read module reads label information and label information is transmitted to retrospective retrival data
Label information and DNA testing result are transmitted to cloud, the retrospect inspection by uploading module, the retrospective retrival data uploading module
Rope data reception module receives safflower production, processing, storage, transaction, logistics, the information of terminal and detection.
The DNA chip and retrospect label that safflower DNA Internet of Things anti-counterfeiting system uses, have in biological detection experimental ability
Medicine trading company, large-scale transaction place, hospital etc. can be used for quickly detecting by DNA chip, and pass through DNA big data analysis
Identify it is true and false, carry out it is anti-fake.Not having pharmacy, consumer of biological detection experimental ability etc. can be chased after by tracing label
It traces back, checks safflower production, processing, storage, transaction, logistics, the information of terminal and detection, cloud can analyse whether there is exception simultaneously
Analysis result is issued into inquiry.By big data make trace tag queries to data as time and space change, i.e.,
Just identical retrospect label is copied, abnormal request for information also can be by system discovery.Realization has efficiently controlled fake producer's
Duplication forms preferably counterfeit protection measures.
The retrospect label is RFID RFID tag.The retrospect label is arranged in DNA chip.DNA retrieval is eventually
End and retrospective retrival terminal can be a terminal, i.e., the dress handled simultaneous with RFID radio frequency recognition function and networking data
It sets, such as with mobile phone, the terminal of RFID identification function.
Agent, logistics, consumer need that proof of identity is registered and carried out on beyond the clouds, and the agent, logistics are to cloud
The data of correspondence ID in the storage module of end are surrounded by reading permission, number of the consumer to the correspondence ID in the storage module of cloud
It reads permission according to being surrounded by and forbids the user read permission in addition to the manager of cloud.Reading every time in this way has corresponding identity
Information produces exception reporting if the consumer of terminal repeatedly inquires, and informs the larger possible generation retrospect label of consumer
The case where being replicated, if there is consumer retracts commodity, sales end can typing correlation sales information, reduce the possibility of cloud erroneous judgement
Property.
After each ID data packet starts equipped with timing routine, self-generating ID on the cloud storage module after a certain period of time,
Retrospective retrival terminal can not retrieve.Retrospect label can be set according to actual conditions according to the characteristic of itself product in medicinal material manufacturer
The validity period of data retrieval, retrieval number, timeliness one arrive, then trace label automatic damage, even if personator has recycled retrospect mark
Label, and scheme in vain.
Medicinal material manufacturer can according to the actual situation nullify specified retrospect tag ID when specific.Make to trace label
The validity of ID among the control of medicinal material manufacturer, allows fake producer to have no exploits always.
Cloud manager needs the safety of strict control cloud data, can set management code key, it is ensured that cloud data are tight
Lattice rest in production firm.
The above description is only an embodiment of the present invention, the protection scope being not intended to limit the invention；Protection of the invention
Range is limited by the claim in claims, and all according to made equivalence changes and modification is invented, all in this hair
Within the protection scope of bright patent.
Claims (10)
1. a kind of safflower DNA Rapid identification Internet of Things anti-fake traceability system, it is characterised in that：Including cloud, DNA chip, identification
Device, retrospect label, DNA searching terminal, retrospective retrival terminal；
The cloud includes storage module, analysis module, cloud information receiving module, cloud message output module, the storage
Module stores safflower DNA database and safflower production, processing, storage, transaction, logistics, the information of terminal and detection, described
Cloud information receiving module receives the data of DNA searching terminal and retrospective retrival terminal by network and sends data to analysis
Module, the analysis module analyze the received data of the information receiving module, receive related data from storage module, to score
Analyse DNA data, by DNA analysis result data and analysis safflower production, processing, storage, transaction, logistics, terminal and detection
Data and analysis result are simultaneously transmitted to message output module by information, and the cloud message output module transmits information to DNA
Searching terminal, retrospective retrival terminal；
The DNA chip, including solid phase support object, gene probe；
Each retrospect label includes corresponding and unique id information, and the id information, processing, storage, is handed at safflower production
Easily, logistics, terminal and the information of detection form the storage module in corresponding ID data packet storage beyond the clouds；
DNA searching terminal, including DNA data uploading module, DNA data reception module, the DNA data uploading module is by DNA
Chip detection data is transmitted to cloud, and the DNA data reception module receives cloud DNA analysis result；
Retrospective retrival terminal includes tracing label information read module, retrospective retrival data uploading module, retrospective retrival data to connect
Module is received, the retrospect label information read module reads label information and label information is transmitted to retrospective retrival data and uploads
Label information and DNA testing result are transmitted to cloud, the retrospective retrival number by module, the retrospective retrival data uploading module
Safflower production, processing, storage, transaction, logistics, the information of terminal and detection are received according to receiving module.
2. safflower DNA Rapid identification Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：It is described red
Flower DNA database is established in the following manner：
(1) certified products safflower sample is subjected to batch extracting；
(2) by the safflower DNA sample and DNA chip progress hybridization reaction after extraction；
It (3) will be in hybridization reaction result input database；
(4) by it is a variety of without brand, different size, different sources, different batches safflower sample pass sequentially through step (1),
(2), (3) are detected, and form safflower DNA database.
3. safflower DNA Rapid identification Internet of Things anti-fake traceability system according to claim 2, it is characterised in that：Step (1)
In, safflower sample is extracted by CTAB method, and detailed process is as follows：4ml CTAB extracting solution and 80ul sulfydryl are added in centrifuge tube
Ethyl alcohol preheats in 65 ± 5 DEG C of water-baths, takes safflower sample, and liquid nitrogen grinding is at powder, in the centrifuge tube after being transferred to preheating, envelope
Mouthful, 30 ± 10 minutes are kept the temperature in 65 ± 5 DEG C of water-baths, takes out centrifuge tube, 4ml phenol: three chloromethanes is added in fast cooling to room temperature
Alkane: 25: 24: 1 mixed solution of isoamyl alcohol mixes, and forms mixed emulsion；12000r/min is centrifuged 10min, takes supernatant, adds
Enter the chloroform of 4ml: the mixed solution of isoamyl alcohol 24: 1, in being placed at room temperature for 10-20min after mixing, 12000r/min from
Heart 10min removes supernatant, and precipitating successively uses dehydrated alcohol, 70% ethanol washing, and TE buffer and final concentration of 50 μ g/ is added
The RNase of ml obtains safflower DNA sample in 37 DEG C of heat preservation 1h.
4. safflower DNA Rapid identification Internet of Things anti-fake traceability system according to claim 1, which is characterized in that the base
Because probe includes following probe：
Probe one：GCGTGATATGCTTAAGTTCAG；
Probe two：AGTCCTACCTGATTTGAGGTC；
Probe three：TCTTTGAAGCATGAAC；
Probe four：CAGTAGGAGATAATTATC；
Probe five：TCGAGATAATTATCACAC；
Probe six：ATGAGCGTTCAAAGATTCGT.
5. safflower DNA Rapid identification Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：The DNA
Chip is surface plasma resonant sensor chip, and the solid phase support object is silicon chip, the attachment of solid phase support object surface
Layer of metal film, the gene probe are fixed on metallic film surface.
6. safflower DNA Rapid identification Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：It is described to chase after
The label that traces back is RFID RFID tag.
7. safflower DNA Rapid identification Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：It is described to chase after
The label that traces back is arranged in DNA chip.
8. safflower DNA Rapid identification Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：Agent,
Logistics, consumer need to register and carry out on beyond the clouds proof of identity, and the agent, logistics are to pair in the storage module of cloud
Answer the data of ID to be surrounded by reading permission, the consumer data of the correspondence ID in the storage module of cloud are surrounded by read permission with
And forbid the user read permission in addition to the manager of cloud.
9. safflower DNA Rapid identification Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：The cloud
Hold storage module on each ID data packet be equipped with timing routine, self-generating ID start after after a certain period of time, retrospective retrival terminal without
Method retrieves.
10. a kind of anti-fake tracing to the source of the described in any item safflower DNA Rapid identification Internet of Things anti-fake traceability systems of claim 1-9
Method, which is characterized in that including following：
Personnel with detectability, detect in the following ways：
(1) DNA sample that tested safflower sample is extracted by CTAB method, carries out hybridization reaction for DNA sample and DNA chip, obtains
Take response data；
(2) by retrospective retrival terminal scanning retrospect label, response data is uploaded to cloud and cloud by DNA searching terminal
Data in DNA database compare, and obtain true and false result；
(3) it will test place, time, result are transmitted to cloud retrospect label safflower sample and correspond to ID data corresponding to batch
Packet；
Do not have the personnel of detectability, identifies in the following ways：
(1) by retrospective retrival terminal scanning retrospect label, safflower production, processing, storage, transaction, logistics, terminal and inspection are obtained
The information of survey；
(2) cloud carries out analysis according to historical query information and analysis result is sent to retrospective retrival terminal.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810432120.7A CN108898394A (en) | 2018-05-08 | 2018-05-08 | A kind of safflower DNA Rapid identification Internet of Things anti-fake traceability system and anti-fake source tracing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810432120.7A CN108898394A (en) | 2018-05-08 | 2018-05-08 | A kind of safflower DNA Rapid identification Internet of Things anti-fake traceability system and anti-fake source tracing method |
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|---|---|
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ID=64342667
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| Country | Link |
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2018
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