CN108645797A - Donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems and anti-fake source tracing method - Google Patents
Donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems and anti-fake source tracing method Download PDFInfo
- Publication number
- CN108645797A CN108645797A CN201810433418.XA CN201810433418A CN108645797A CN 108645797 A CN108645797 A CN 108645797A CN 201810433418 A CN201810433418 A CN 201810433418A CN 108645797 A CN108645797 A CN 108645797A
- Authority
- CN
- China
- Prior art keywords
- dna
- donkey
- hide gelatin
- data
- clouds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 108010010803 Gelatin Proteins 0.000 title claims abstract description 105
- 229920000159 gelatin Polymers 0.000 title claims abstract description 105
- 239000008273 gelatin Substances 0.000 title claims abstract description 105
- 235000019322 gelatine Nutrition 0.000 title claims abstract description 105
- 235000011852 gelatine desserts Nutrition 0.000 title claims abstract description 105
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000003860 storage Methods 0.000 claims abstract description 43
- 238000000018 DNA microarray Methods 0.000 claims abstract description 39
- 238000004458 analytical method Methods 0.000 claims abstract description 37
- 238000001514 detection method Methods 0.000 claims abstract description 34
- 238000004519 manufacturing process Methods 0.000 claims abstract description 20
- 238000012545 processing Methods 0.000 claims abstract description 18
- 239000000523 sample Substances 0.000 claims description 59
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 20
- 239000000047 product Substances 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 239000011324 bead Substances 0.000 claims description 10
- 238000009396 hybridization Methods 0.000 claims description 10
- 239000000377 silicon dioxide Substances 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 241000283074 Equus asinus Species 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 8
- 238000012360 testing method Methods 0.000 claims description 8
- 241000283073 Equus caballus Species 0.000 claims description 7
- 241001494479 Pecora Species 0.000 claims description 7
- 239000000839 emulsion Substances 0.000 claims description 7
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 230000004044 response Effects 0.000 claims description 6
- 239000007790 solid phase Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 235000019441 ethanol Nutrition 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 5
- 238000007689 inspection Methods 0.000 claims description 5
- 239000002184 metal Substances 0.000 claims description 5
- 229910052751 metal Inorganic materials 0.000 claims description 5
- GHKCSRZBNZQHKW-UHFFFAOYSA-N 1-sulfanylethanol Chemical class CC(O)S GHKCSRZBNZQHKW-UHFFFAOYSA-N 0.000 claims description 4
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims description 4
- 239000007984 Tris EDTA buffer Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical group [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- BKHZIBWEHPHYAI-UHFFFAOYSA-N chloroform;3-methylbutan-1-ol Chemical compound ClC(Cl)Cl.CC(C)CCO BKHZIBWEHPHYAI-UHFFFAOYSA-N 0.000 claims description 3
- 238000013461 design Methods 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 claims 1
- 239000002344 surface layer Substances 0.000 claims 1
- 230000002159 abnormal effect Effects 0.000 abstract description 5
- 238000007405 data analysis Methods 0.000 abstract description 3
- 230000008859 change Effects 0.000 abstract description 2
- 108020004414 DNA Proteins 0.000 description 71
- 239000003814 drug Substances 0.000 description 7
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 206010046788 Uterine haemorrhage Diseases 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 2
- 241001550206 Colla Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 206010013954 Dysphoria Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000034507 Haematemesis Diseases 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 241000590428 Panacea Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 240000005373 Panax quinquefolius Species 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 206010046910 Vaginal haemorrhage Diseases 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 210000003056 antler Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000003574 free electron Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000035861 hematochezia Diseases 0.000 description 1
- 208000006750 hematuria Diseases 0.000 description 1
- 208000006278 hypochromic anemia Diseases 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000006855 networking Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 229940126532 prescription medicine Drugs 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
- G06K17/00—Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations
- G06K17/0022—Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations arrangements or provisions for transferring data to distant stations, e.g. from a sensing device
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
- G06Q30/00—Commerce
- G06Q30/018—Certifying business or products
- G06Q30/0185—Product, service or business identity fraud
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04L—TRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
- H04L67/00—Network arrangements or protocols for supporting network services or applications
- H04L67/01—Protocols
- H04L67/12—Protocols specially adapted for proprietary or special-purpose networking environments, e.g. medical networks, sensor networks, networks in vehicles or remote metering networks
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Business, Economics & Management (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Theoretical Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Entrepreneurship & Innovation (AREA)
- Development Economics (AREA)
- Pathology (AREA)
- Computer Networks & Wireless Communication (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Signal Processing (AREA)
- Computing Systems (AREA)
- Accounting & Taxation (AREA)
- Medical Informatics (AREA)
- Economics (AREA)
- Finance (AREA)
- Marketing (AREA)
- Strategic Management (AREA)
- General Business, Economics & Management (AREA)
- Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
Abstract
The invention belongs to donkey-hide gelatin to identify anti-counterfeit field, and in particular to a kind of donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems and anti-fake source tracing method.The DNA chip and retrospect label used including high in the clouds, DNA chip, identification apparatus, retrospect label, DNA searching terminals, retrospective retrival terminal donkey-hide gelatin DNA Internet of Things anti-counterfeiting systems, have being used for quickly detecting by DNA chip for biological detection experimental ability, and it is true and false by the discriminating of DNA big data analysis, it carries out anti-fake.Do not have being traced by tracing label for biological detection experimental ability, checks that the information of donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and detection, high in the clouds can analyse whether to exist abnormal and analysis result issued inquiry.By big data make retrospect tag queries to data change with time and space, even if copying identical retrospect label, abnormal request for information also can be by system discovery.It realizes the duplication for having efficiently controlled fake producer, forms preferably counterfeit protection measures.
Description
Technical field
The invention belongs to donkey-hide gelatin to identify anti-counterfeit field, and in particular to a kind of donkey-hide gelatin DNA Rapid identification Internet of Things is anti-fake to trace to the source
System and anti-fake source tracing method.
Background technology
The drying skin or fresh hide that donkey-hide gelatin (Asini Corii Colla) is equid donkey Equus asinus L. are through decocting
Boil, concentrate made of solid gum, and ginseng, pilose antler and be known as " nourishing Triratna ", first recorded in《Sheng Nong's herbal classic》, it is classified as top grade,
Tao Hongjing is said:" going out Donga, therefore named donkey-hide gelatin ".Donkey-hide gelatin is sweet in flavor, mild-natured, return lung, liver and kidney channel.With enriching yin of enriching blood, moisturizes, stops blooding
The effect of, it is respectfully called by ancient Chinese medicine doctor enrich blood " panacea ".Cure mainly blood deficiency chlorosis, dizziness palpitaition, impotence weakness, dysphoria and insomnia, void
It is moved in wind, xeropulmonary cough, consumptive disease cough spitting of blood, haematemesis hematuria, uterine bleeding of having blood in stool, vaginal bleeding during pregnancy.
2015 editions《Chinese Pharmacopoeia》Middle clear stipulaties donkey-hide gelatin be donkey-hide gelatin made of the skin tanning of equid donkey be certified products Ah
Glue.However as donkey-hide gelatin market development, endure that collagen material is in short supply and rise in price, since donkey-hide gelatin effect is unique, market supplies
It should not ask, adulterated low-quality goods emerges one after another, and is constantly exposed by the media.These donkey-hide gelatin inferior mostly use ox, horse, pig, sheep etc.
Animal Skin, bone, connective tissue tanning, are visually difficult to differentiate.Donkey-hide gelatin inferior circulate in the market not only to consumer bring through
The loss of Ji and health, also seriously affects the sound development in certified products donkey-hide gelatin market.
Traditional is anti-fake mainly using physics modes such as anti-counterfeiting label, false proof card, anti-counterfeit packages, by the straight of assessor
Connect the subjective mode such as observation, touch, number inquiry.As patent CN00213421.7 discloses a kind of Anti-false cylindrical paper container, including
Cover, tank bottom and tubular composite paper tank body, cover, tank bottom are fixed together with tank body, and tank body is equipped with by continuous
The point off circumference that breaking point or geosutures are constituted.This structure is easily copied.As patent CN200410012982.2 discloses one
Kind G 2 encoding method for commodity is the substance being applied in each field, commodity, equipment and other coding methods.Coding is divided into two
Grade, level encoder are merchandise classification, general important name and alias, and two level is encoded to the general important name of commodity or alias by level encoder
The commercial specification model that derives from, measurement unit, production firm, packaging label, authentication code, other marks, condition of storage
The relationship of equal codes, level encoder and two level coding is not only independent but also complementary two generations relationship, can be applied independently or together.Its
It is identified, as medical mark includes Chinese medicine mark, prescription medicine mark, imported medicine identifies, special management medicine identifies, country is basic
Medicine identifies, but coding can be replicated easily.Therefore, as long as product is once dispatch from the factory, fake producer is gratifying to be intended to be imitated for ground
It makes, is not broken away from always by counterfeit destiny.
Invention content
The present invention is in view of the above-mentioned problems, providing a kind of donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems and anti-fake tracing back
Source method.
The technical solution used in the present invention is as follows：A kind of donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system, including
High in the clouds, DNA chip, identification apparatus, retrospect label, DNA searching terminals, retrospective retrival terminal；
The high in the clouds includes storage module, analysis module, high in the clouds information receiving module, high in the clouds message output module, described
Storage module stores the information of donkey-hide gelatin DNA databases and donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and detection,
The high in the clouds information receiving module receives the data of DNA searching terminals and retrospective retrival terminal by network and sends data to
Analysis module, the data of the analysis module analysis described information receiving module reception receive related data from storage module, are right
Than analysis DNA data, by DNA analysis result data and analysis donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and inspection
Data and analysis result are simultaneously transmitted to message output module by the information of survey, and the high in the clouds message output module transmits information to
DNA searching terminals, retrospective retrival terminal；
The DNA chip, including solid phase support object, gene probe, the gene probe include according to donkey, pig, ox, sheep,
The probe of the gene design of horse, rabbit, including it is as follows：
Donkey, AACGTAGGTCTGACGTGACTCCCCGA；
Pig, CCTATATACCGCCATCTTC；
Ox, GAGGAGCCTGTTCCGTAATCGAT；
Sheep, TCAAGCACACTACAAAGTATCGC；
Horse, GTAAATTAAGAAAGAGAGCTTAATT；
Rabbit, ACACCTTGCTAGGCCACACCCACGGG；
Can also include existing known many animals DNA detection probes；
Each retrospect label includes corresponding and unique id information, the id information, donkey-hide gelatin production, processing, store,
Transaction, logistics, the information of terminal and detection form the storage module in corresponding ID data packets storage beyond the clouds；
DNA searching terminals, including DNA data uploading module, DNA data reception modules, the DNA data uploading module will
DNA chip detection data is transmitted to high in the clouds, and the DNA data reception modules receive high in the clouds DNA analysis as a result, DNA searching terminals
Can be mobile phone, computer；
Retrospective retrival terminal includes retrospect label information read module, retrospective retrival data uploading module, retrospective retrival number
According to receiving module, the retrospect label information read module reads label information and label information is transmitted to retrospective retrival data
Label information and DNA testing results are transmitted to high in the clouds, the retrospect inspection by uploading module, the retrospective retrival data uploading module
Rope data reception module receives the information of donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and detection.
The donkey-hide gelatin DNA databases are established in the following manner：
(1) certified products donkey-hide gelatin sample is extracted into donkey-hide gelatin DNA sample；
(2) the donkey-hide gelatin DNA sample after extraction is subjected to hybridization reaction with DNA chip；
It (3) will be in hybridization reaction result input database；
(4) a variety of donkey-hide gelatin samples without brand, different size, different sources, different batches are passed sequentially through into step
(1), (2), (3) are detected, and form donkey-hide gelatin DNA databases.
In step (1), donkey-hide gelatin sample is extracted by silica bead method, and detailed process is as follows：
(a) 4ml CTAB extracting solutions and 80ul mercaptoethanols are added in centrifuge tube, is preheated in 65 ± 5 DEG C of water-baths,
Donkey-hide gelatin sample is taken, powder is ground into, is transferred in the centrifuge tube after preheating, sealing keeps the temperature 30 ± 10 points in 65 ± 5 DEG C of water-baths
Clock takes out centrifuge tube, and 4ml chloroform-isoamyl alcohol mixed solutions are added in fast cooling to room temperature, and mixing forms mixed emulsion；
(b) 100ul silica bead suspension is added into mixed emulsion, mixing stands 10 minutes, 4 degree of conditions of 8000rpm15s
Lower centrifugation goes supernatant, precipitation to be washed 2 times with 1ml cleaning solutions respectively, and 1ml ice ethyl alcohol washs 2 times, 60 DEG C of drying 5min；
(c) after addition 100ul TE buffer solutions mix well, 10 minutes is kept the temperature in 56 degree of water-baths, is then centrifuged for
12000rpm 5min take the donkey-hide gelatin DNA sample that supernatant is required.
The DNA chip is surface plasma resonant sensor chip, and the solid phase support object is silicon chip, the solid phase
Layer of metal film is adhered on supporter surface, and the gene probe is fixed on metallic film surface.Utilize surface plasma resonance
(surface plasmon resonance, SPR) biosensor technology quickly detects the DNA of donkey-hide gelatin.Surface plasma is total
Shake (Surface Plasmon Resonance, SPR) be a kind of optical physics phenomenon.When a branch of P polarization light is in certain angle
It is incident on prism end face in range, surface plasma-wave will be generated at the interface of prism and metallic film (Au or Ag).Work as incidence
When the propagation constant of light wave and the propagation constant of surface plasma-wave match, free electron in metal film is caused to generate resonance,
That is surface plasma resonance.When analysis, first one layer of biomolecule is fixed in censorchip surface and identify film, then by sample to be tested
Chip surface is flowed through, if there is the molecule that can identify membrane interaction with the biomolecule of chip surface in sample, gold can be caused
Film surface variations in refractive index eventually leads to the variation of the angles SPR and obtains concentration, the parent of analyte by detecting SPR angle changes
With the information such as power, kinetic constant and specificity.Occur both when donkey-hide gelatin DNA sample after extraction is injected into chip surface special
The opposite sex combines, and changes so as to cause chip surface optical parameter, and show as electronic signals, specific bond determinand
Amount it is different, the response intensity of signal is different.
The retrospect label is RFID RFID tags.Radio frequency identification, RFID (Radio
FrequencyIdentification) technology, also known as radio frequency identification are a kind of communication technologys, are commonly called as electronic tag.It can
Specific objective is identified by radio signals and reads and writes related data, without establishing machinery between identifying system and specific objective
Or optical contact.Convenient for identification, make the information Rapid input of production, processing, storage, transaction, logistics, terminal and detection.Also may be used
It to take bar code, Quick Response Code isotype, but needs to be scanned one by one, is unfavorable for the typing of high-volume information.
The retrospect label is arranged in DNA chip.Directly testing result can be chased after by scanning after tester's detection
The label that traces back connects high in the clouds, detection data is transmitted to high in the clouds, and obtain high in the clouds analysis result, coherent detection data are saved in cloud
On end.
Agent, logistics, consumer need that proof of identity is registered and carried out on beyond the clouds, and the agent, logistics are to cloud
The data of correspondence ID in the storage module of end are surrounded by reading permission, number of the consumer to the correspondence ID in the storage module of high in the clouds
It reads permission according to being surrounded by and forbids the user read permission in addition to the manager of high in the clouds.Reading every time in this way has corresponding identity
Information produces exception reporting if the consumer of terminal repeatedly inquires, and retrospect label may be occurred by informing that consumer is larger
The case where being replicated, if there is consumer retracts commodity, sales end can typing correlation sales information, reduce the possibility of high in the clouds erroneous judgement
Property.
Each ID data packets are equipped with consumer's reading times restricted program on the high in the clouds storage module, are set to from identity and disappear
Restriction ID data packets are only capable of being registered the certain number of user's reading after the user of expense person reads ID data packets for the first time.
After each ID data packets start equipped with timing routine, self-generating ID on the high in the clouds storage module after a certain period of time,
Retrospective retrival terminal can not retrieve.High in the clouds manager can be according to the characteristic of donkey-hide gelatin product, and be set according to actual conditions DNA has
The effect phase avoids the retrospect label on expired product or expired product from shifting and uses.
A kind of anti-fake source tracing method of above-mentioned donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems, including it is following：
Personnel with detectability, detect in the following ways：
(1) DNA sample for being tested donkey-hide gelatin sample is extracted by silica bead method, and DNA sample with DNA chip hybridize instead
It answers, obtains response data；
(2) by retrospective retrival terminal scan retrospect label, by response data by DNA searching terminals be uploaded to high in the clouds with
Data in the DNA databases of high in the clouds are compared, and obtain true and false result；
(3) detection site, time, result are transmitted to high in the clouds retrospect label donkey-hide gelatin sample and correspond to ID numbers corresponding to batch
According to packet；
The personnel for not having detectability, identify in the following ways：
(1) retrospect label is scanned by retrospective retrival terminal, obtains donkey-hide gelatin production, processing, storage, transaction, logistics, terminal
With the information of detection；
(2) high in the clouds according to historical query information analyze and analysis result is sent to retrospective retrival terminal.
Beneficial effects of the present invention are as follows：The DNA chip and retrospect label that donkey-hide gelatin DNA Internet of Things anti-counterfeiting systems use, tool
The Chinese medicine trading company of standby biological detection experimental ability, large-scale transaction place, hospital etc. can quickly be examined by DNA chip
It surveys, and true and false by the discriminating of DNA big data analysis, carries out anti-fake.Do not have pharmacy, the consumer etc. of biological detection experimental ability
It can be traced by tracing label, check the information of donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and detection, cloud
End can analyse whether to exist abnormal and analysis result is issued inquiry.By big data make the data that retrospect tag queries arrive with
It time and space and changes, even if copying identical retrospect label, abnormal request for information also can be by system discovery.It realizes
The duplication of fake producer has been efficiently controlled, preferably counterfeit protection measures are formed.
Description of the drawings
Fig. 1 is the schematic diagram of donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems.
Fig. 2 is donkey-hide gelatin DNA rapid identification method flow charts.
Specific implementation mode
Below in conjunction with the accompanying drawings and specific embodiment, the present invention can be better described.
Embodiment one：
DNA chip is prepared, detailed process is as follows：
Can be Au or Ag 1. adhering to the metal film of one layer of 150nm by metal coating technique in silicon chip surface；
2. in probe genetic fragment sulfhydrylation, then by the probe points of different sulfhydrylations to different zones, in 4 DEG C of conditions
It is lower keep 12 hours, including according to donkey, pig, ox, sheep, horse,
The probe of the gene design of rabbit：
Donkey, AACGTAGGTCTGACGTGACTCCCCGA；
Pig, CCTATATACCGCCATCTTC；
Ox, GAGGAGCCTGTTCCGTAATCGAT；
Sheep, TCAAGCACACTACAAAGTATCGC；
Horse, GTAAATTAAGAAAGAGAGCTTAATT；
Rabbit, ACACCTTGCTAGGCCACACCCACGGG.
3. then washing the unbonded probe of metallic film surface with deionized water, then dried up with nitrogen.
There is good stability by DNA chip prepared by the above method, can for a long time be flowed on the market with product
Logical, the DNA probe on surface is designed for the DNA sequence dna of donkey-hide gelatin and its common adulterant, some existing DNA of plants are being added
Probe has specificity, good test effect by the DNA information of probe test donkey-hide gelatin sample.
Embodiment two：
Donkey-hide gelatin DNA databases are built, detailed process is as follows：
1. certified products donkey-hide gelatin sample is extracted by silica bead method, detailed process is as follows：
(a) 4ml CTAB extracting solutions and 80ul mercaptoethanols are added in centrifuge tube, is preheated in 65 ± 5 DEG C of water-baths,
Donkey-hide gelatin sample is taken, powder is ground into, is transferred in the centrifuge tube after preheating, sealing keeps the temperature 30 ± 10 points in 65 ± 5 DEG C of water-baths
Clock takes out centrifuge tube, and 4ml chloroform-isoamyl alcohol mixed solutions are added in fast cooling to room temperature, and mixing forms mixed emulsion；
(b) 100ul silica bead suspension is added into mixed emulsion, mixing stands 10 minutes, 4 degree of conditions of 8000rpm15s
Lower centrifugation goes supernatant, precipitation to be washed 2 times with 1ml cleaning solutions respectively, and 1ml ice ethyl alcohol washs 2 times, 60 DEG C of drying 5min；
(c) after addition 100ul TE buffer solutions mix well, 10 minutes is kept the temperature in 56 degree of water-baths, is then centrifuged for
12000rpm 5min take the donkey-hide gelatin DNA sample that supernatant is required.
2. the donkey-hide gelatin DNA sample after extraction is carried out hybridization reaction with DNA chip：Donkey-hide gelatin DNA
Sample injection is to DNA chip surface, hybridization reaction 5 hours at 45 DEG C.
3. the spr signal of each probe area is obtained, in input database；
4. a variety of donkey-hide gelatin samples without brand, different size, different sources, different batches are passed sequentially through step 1 to 3
It is detected, forms donkey-hide gelatin DNA databases.
The DNA information in donkey-hide gelatin sample is extracted by step 1, relatively other methods, extracting concentration is high, relatively complete
It is whole, hybridization reaction is carried out with DNA chip, reaction is sensitiveer, and conjugation is relatively high, observes the data in DNA databases, finds
Without brand, different size, different sources, different batches donkey-hide gelatin sample data it is roughly the same, have part have differences.
Embodiment three：
Check that the validity of DNA chip and donkey-hide gelatin DNA databases, testing staff do not know the true and false of tested donkey-hide gelatin sample：
1. tested donkey-hide gelatin sample is extracted by silica bead method, detailed process is as follows：
(a) 4ml CTAB extracting solutions and 80ul mercaptoethanols are added in centrifuge tube, is preheated in 65 ± 5 DEG C of water-baths,
Donkey-hide gelatin sample is taken, powder is ground into, is transferred in the centrifuge tube after preheating, sealing keeps the temperature 30 ± 10 points in 65 ± 5 DEG C of water-baths
Clock takes out centrifuge tube, and 4ml chloroform-isoamyl alcohols (24: 1) mixed solution is added in fast cooling to room temperature, and mixing forms mixing breast
Liquid；
(b) 100ul silica bead suspension is added into mixed emulsion, mixing stands 10 minutes, 4 degree of items of 8000rpm 15s
It is centrifuged under part, supernatant, precipitation is gone to be washed 2 times with 1ml cleaning solutions respectively, 1ml ice ethyl alcohol washs 2 times, 60 DEG C of drying 5min；
(c) after addition 100ul TE buffer solutions mix well, 10 minutes is kept the temperature in 56 degree of water-baths, is then centrifuged for
12000rpm 5min take the donkey-hide gelatin DNA sample that supernatant is required.
2. the donkey-hide gelatin DNA sample after extraction is carried out hybridization reaction with DNA chip：Donkey-hide gelatin DNA sample is injected into DNA chip
Surface, hybridization reaction 5 hours at 45 DEG C.
3. obtaining the spr signal of each probe area, the spr signal data of detection and donkey-hide gelatin DNA databases are compared,
The sample that similarity is more than 90% is true, and the sample being less than is false.
4. a variety of donkey-hide gelatin samples are passed sequentially through step 1 to be detected to 3, the identification true and false is obtained as a result, detection accuracy
It is 100%.Illustrate that DNA chip and donkey-hide gelatin DNA databases can be effectively used for authenticity of hide glue discriminating.
Through this embodiment, it may be said that bright DNA chip and donkey-hide gelatin DNA databases can be effectively used for authenticity of hide glue discriminating.It is pseudo-
The DNA chip detection data of product donkey-hide gelatin sample and donkey-hide gelatin DNA database similarities are below 85%, and certified products donkey-hide gelatin sample
DNA chip detection data is above 95% with donkey-hide gelatin DNA database similarities, can obviously distinguish, pass through DNA chip in this way
Testing result error is small.It high in the clouds can be between 85%-95% to the setting of certified products similarity limit.
Example IV：
A kind of donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system, including high in the clouds, DNA chip, identification apparatus, retrospect
Label, DNA searching terminals, retrospective retrival terminal；
The high in the clouds includes storage module, analysis module, high in the clouds information receiving module, high in the clouds message output module, described
Storage module stores the information of donkey-hide gelatin DNA databases and donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and detection,
The high in the clouds information receiving module receives the data of DNA searching terminals and retrospective retrival terminal by network and sends data to
Analysis module, the data of the analysis module analysis described information receiving module reception receive related data from storage module, are right
Than analysis DNA data, by DNA analysis result data and analysis donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and inspection
Data and analysis result are simultaneously transmitted to message output module by the information of survey, and the high in the clouds message output module transmits information to
DNA searching terminals, retrospective retrival terminal；
The DNA chip is the DNA chip prepared by embodiment one；
Each retrospect label includes corresponding and unique id information, the id information, donkey-hide gelatin production, processing, store,
Transaction, logistics, the information of terminal and detection form the storage module in corresponding ID data packets storage beyond the clouds；
DNA searching terminals, including DNA data uploading module, DNA data reception modules, the DNA data uploading module will
DNA chip detection data is transmitted to high in the clouds, and the DNA data reception modules receive high in the clouds DNA analysis result；
Retrospective retrival terminal includes retrospect label information read module, retrospective retrival data uploading module, retrospective retrival number
According to receiving module, the retrospect label information read module reads label information and label information is transmitted to retrospective retrival data
Label information and DNA testing results are transmitted to high in the clouds, the retrospect inspection by uploading module, the retrospective retrival data uploading module
Rope data reception module receives the information of donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and detection.
The DNA chip and retrospect label that donkey-hide gelatin DNA Internet of Things anti-counterfeiting systems use, have in biological detection experimental ability
Medicine trading company, large-scale transaction place, hospital etc. can be used for quickly detecting by DNA chip, and pass through DNA big data analysis
Differentiate it is true and false, carry out it is anti-fake.Not having pharmacy, consumer of biological detection experimental ability etc. can be chased after by tracing label
It traces back, checks that the information of donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and detection, high in the clouds can analyse whether there is exception simultaneously
Analysis result is issued into inquiry.By big data make retrospect tag queries to data change with time and space, i.e.,
Just identical retrospect label is copied, abnormal request for information also can be by system discovery.Realization has efficiently controlled fake producer's
It replicates, forms preferably counterfeit protection measures.
The retrospect label is RFID RFID tags.The retrospect label is arranged in DNA chip.DNA retrievals are eventually
End and retrospective retrival terminal can be a terminal, i.e., the dress handled simultaneous with RFID radio frequency recognition functions and networking data
It sets, such as mobile phone, terminal with RFID identification function.
Agent, logistics, consumer need that proof of identity is registered and carried out on beyond the clouds, and the agent, logistics are to cloud
The data of correspondence ID in the storage module of end are surrounded by reading permission, number of the consumer to the correspondence ID in the storage module of high in the clouds
It reads permission according to being surrounded by and forbids the user read permission in addition to the manager of high in the clouds.Reading every time in this way has corresponding identity
Information produces exception reporting if the consumer of terminal repeatedly inquires, and retrospect label may be occurred by informing that consumer is larger
The case where being replicated, if there is consumer retracts commodity, sales end can typing correlation sales information, reduce the possibility of high in the clouds erroneous judgement
Property.
After each ID data packets start equipped with timing routine, self-generating ID on the high in the clouds storage module after a certain period of time,
Retrospective retrival terminal can not retrieve.Medicinal material manufacturer can be set according to actual conditions retrospect label according to the characteristic of itself product
The term of validity of data retrieval, retrieval number, timeliness one arrive, then trace label automatic damage, even if personator has recycled retrospect mark
Label, and scheme in vain.
Medicinal material manufacturer can be nullified specified retrospect tag ID when specific according to actual conditions.Make retrospect label
The validity of ID among the control of medicinal material manufacturer, allows fake producer to have no exploits always.
High in the clouds manager needs the safety of stringent control high in the clouds data, can set management secret key, it is ensured that high in the clouds data are tight
Lattice rest in production firm.
Example the above is only the implementation of the present invention is not used for limiting the scope of the invention；The protection of the present invention
Range is limited by the claim in claims, and every according to equivalence changes made by invention and modification, all in this hair
Within the protection domain of bright patent.
Claims (10)
1. a kind of donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system, it is characterised in that：Including high in the clouds, DNA chip, identification
Device, retrospect label, DNA searching terminals, retrospective retrival terminal；
The high in the clouds includes storage module, analysis module, high in the clouds information receiving module, high in the clouds message output module, the storage
Module stores the information of donkey-hide gelatin DNA databases and donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and detection, described
High in the clouds information receiving module receives the data of DNA searching terminals and retrospective retrival terminal by network and sends data to analysis
Module, the data of the analysis module analysis described information receiving module reception receive related data from storage module, to score
Analyse DNA data, by DNA analysis result data and the production of analysis donkey-hide gelatin, processing, storage, transaction, logistics, terminal and detection
Data and analysis result are simultaneously transmitted to message output module by information, and the high in the clouds message output module transmits information to DNA
Searching terminal, retrospective retrival terminal；
The DNA chip, including solid phase support object, gene probe, the gene probe include according to donkey, pig, ox, sheep, horse, rabbit
Gene design probe, including it is as follows：
Donkey, AACGTAGGTCTGACGTGACTCCCCGA；
Pig, CCTATATACCGCCATCTTC；
Ox, GAGGAGCCTGTTCCGTAATCGAT；
Sheep, TCAAGCACACTACAAAGTATCGC；
Horse, GTAAATTAAGAAAGAGAGCTTAATT；
Rabbit, ACACCTTGCTAGGCCACACCCACGGG；
Each retrospect label includes corresponding and unique id information, and the id information, processing, storage, is handed at donkey-hide gelatin production
Easily, logistics, terminal and the information of detection form the storage module in corresponding ID data packets storage beyond the clouds；
DNA searching terminals, including DNA data uploading module, DNA data reception modules, the DNA data uploading module is by DNA
Chip detection data is transmitted to high in the clouds, and the DNA data reception modules receive high in the clouds DNA analysis result；
Retrospective retrival terminal includes tracing label information read module, retrospective retrival data uploading module, retrospective retrival data to connect
Module is received, the retrospect label information read module reads label information and label information is transmitted to retrospective retrival data and uploads
Label information and DNA testing results are transmitted to high in the clouds, the retrospective retrival number by module, the retrospective retrival data uploading module
The information of donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and detection is received according to receiving module.
2. donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：Ah
Glue DNA databases are established in the following manner：
(1) certified products donkey-hide gelatin sample is extracted into donkey-hide gelatin DNA sample；
(2) the donkey-hide gelatin DNA sample after extraction is subjected to hybridization reaction with DNA chip；
It (3) will be in hybridization reaction result input database；
(4) by it is a variety of without brand, different size, different sources, different batches donkey-hide gelatin sample pass sequentially through step (1),
(2), (3) are detected, and form donkey-hide gelatin DNA databases.
3. donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system according to claim 2, it is characterised in that：Step (1)
In, donkey-hide gelatin sample is extracted by silica bead method, and detailed process is as follows：
(a) 4ml CTAB extracting solutions and 80ul mercaptoethanols are added in centrifuge tube, is preheated in 65 ± 5 DEG C of water-baths, takes Ah
Glue sample, is ground into powder, is transferred in the centrifuge tube after preheating, and sealing keeps the temperature 30 ± 10 minutes in 65 ± 5 DEG C of water-baths,
Centrifuge tube is taken out, 4ml chloroform-isoamyl alcohol mixed solutions are added in fast cooling to room temperature, and mixing forms mixed emulsion；
(b) 100ul silica bead suspension is added into mixed emulsion, mixing stands 10 minutes, under the conditions of 4 degree of 8000rpm 15s
Centrifugation goes supernatant, precipitation to be washed 2 times with 1ml cleaning solutions respectively, and 1ml ice ethyl alcohol washs 2 times, 60 DEG C of drying 5min；
(c) after addition 100ul TE buffer solutions mix well, 10 minutes is kept the temperature in 56 degree of water-baths, is then centrifuged for 12000rpm
5min takes the donkey-hide gelatin DNA sample that supernatant is required.
4. donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：The DNA
Chip is surface plasma resonant sensor chip, and the solid phase support object is silicon chip, the attachment of solid phase support object surface
Layer of metal film, the gene probe are fixed on metallic film surface.
5. donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：It is described to chase after
The label that traces back is RFID RFID tags.
6. donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：It is described to chase after
The label that traces back is arranged in DNA chip.
7. donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：Agent,
Logistics, consumer need that proof of identity is registered and carried out on beyond the clouds, and the agent, logistics are to pair in the storage module of high in the clouds
Answer the data of ID to be surrounded by reading permission, the consumer data of the correspondence ID in the storage module of high in the clouds are surrounded by read permission with
And forbid the user read permission in addition to the manager of high in the clouds.
8. donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system according to claim 7, it is characterised in that：The cloud
It holds each ID data packets on storage module to be equipped with consumer's reading times restricted program, the user first of consumer is set to from identity
ID data packets are limited after secondary reading ID data packets to be only capable of being registered the certain number of user's reading.
9. donkey-hide gelatin DNA Rapid identifications Internet of Things anti-fake traceability system according to claim 1, it is characterised in that：The cloud
Hold on storage module each ID data packets to be equipped with timing routine, self-generating ID start after after a certain period of time, retrospective retrival terminal without
Method retrieves.
10. a kind of anti-fake tracing to the source of claim 1-9 any one of them donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems
Method, which is characterized in that including following：
Personnel with detectability, detect in the following ways：
(1) DNA sample for being tested donkey-hide gelatin sample is extracted by silica bead method, and DNA sample and DNA chip are subjected to hybridization reaction, obtained
Take response data；
(2) retrospect label is scanned by retrospective retrival terminal, response data is uploaded to high in the clouds and high in the clouds by DNA searching terminals
Data in DNA databases are compared, and obtain true and false result；
(3) detection site, time, result are transmitted to high in the clouds retrospect label donkey-hide gelatin sample and correspond to ID data corresponding to batch
Packet；
The personnel for not having detectability, identify in the following ways：
(1) retrospect label is scanned by retrospective retrival terminal, obtains donkey-hide gelatin production, processing, storage, transaction, logistics, terminal and inspection
The information of survey；
(2) high in the clouds according to historical query information analyze and analysis result is sent to retrospective retrival terminal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810433418.XA CN108645797A (en) | 2018-05-08 | 2018-05-08 | Donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems and anti-fake source tracing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810433418.XA CN108645797A (en) | 2018-05-08 | 2018-05-08 | Donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems and anti-fake source tracing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108645797A true CN108645797A (en) | 2018-10-12 |
Family
ID=63749413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810433418.XA Withdrawn CN108645797A (en) | 2018-05-08 | 2018-05-08 | Donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems and anti-fake source tracing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108645797A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3859597A1 (en) | 2020-01-31 | 2021-08-04 | U-NICA Systems AG | A computer implemented method and system of surface identification comprising scales |
| CN116934358A (en) * | 2023-09-13 | 2023-10-24 | 澳润(山东)药业有限公司 | Donkey-hide gelatin quality tracing method and donkey-hide gelatin quality tracing system based on information verification |
-
2018
- 2018-05-08 CN CN201810433418.XA patent/CN108645797A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3859597A1 (en) | 2020-01-31 | 2021-08-04 | U-NICA Systems AG | A computer implemented method and system of surface identification comprising scales |
| WO2021152182A1 (en) | 2020-01-31 | 2021-08-05 | U-Nica Systems Ag | A computer implemented method and system of skin identification comprising scales |
| CN116934358A (en) * | 2023-09-13 | 2023-10-24 | 澳润(山东)药业有限公司 | Donkey-hide gelatin quality tracing method and donkey-hide gelatin quality tracing system based on information verification |
| CN116934358B (en) * | 2023-09-13 | 2023-12-15 | 澳润(山东)药业有限公司 | Donkey-hide gelatin quality tracing method and donkey-hide gelatin quality tracing system based on information verification |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105164508B (en) | System and method for acquiring and transmitting measurement result | |
| CN108645797A (en) | Donkey-hide gelatin DNA Rapid identification Internet of Things anti-fake traceability systems and anti-fake source tracing method | |
| CN108844922A (en) | The rapid detection method of drugs in a kind of hair | |
| US20170336428A1 (en) | RFID Tracking Systems And Methods | |
| CN101788564A (en) | Control method for reagent matched with clinical examining and analyzing system | |
| CN108898393A (en) | Chinese medicine DNA Rapid identification Internet of Things anti-fake traceability system and anti-fake source tracing method | |
| Edwards et al. | Modulation of a protein-folding landscape revealed by AFM-based force spectroscopy notwithstanding instrumental limitations | |
| CN109554474A (en) | A kind of method and kit of BCR-ABL fusion quantitative detection | |
| CN107292096A (en) | Clinical examination report auditing system and method based on bar code scan | |
| CN108614956A (en) | A kind of Internet of Things for the stem of noble dendrobium differentiates traceability system and differentiates source tracing method | |
| CN108315388A (en) | A kind of Radix Codonopsis DNA Rapid identification Internet of Things anti-fake traceability systems and anti-fake source tracing method | |
| Forooghi et al. | Authentication and discrimination of tissue origin of bovine gelatin using combined supervised pattern recognition strategies | |
| CN107449907A (en) | The lateral flow chromatography detection card and detection method of a kind of encryption | |
| CN108456718A (en) | A kind of Radix Notoginseng networking anti-fake traceability system based on DNA chip and anti-fake source tracing method | |
| CN108629388A (en) | A kind of Rhizoma Gastrodiae anti-fake traceability system and method based on surface plasma resonance technology | |
| CN108492122A (en) | A kind of Rapid identification Internet of Things anti-fake traceability system and anti-fake source tracing method | |
| CN103606101A (en) | An examining method for discriminating product authenticity based on an article vocal print identification technique | |
| Lam et al. | Evaluation of multi-target immunogenic reagents for the detection of latent and body fluid-contaminated fingermarks | |
| Zoccola et al. | Analytical Methods for the Identification and Quantitative Determination of Wool and Fine Animal Fibers: A Review | |
| CN111261244A (en) | Sample information acquisition and storage system and method | |
| CN108681906A (en) | A kind of radix pseudostellariae Internet of Things anti-fake traceability system and application process | |
| CN108866158A (en) | A kind of ganoderma lucidum DNA Rapid identification Internet of Things anti-fake traceability system and anti-fake source tracing method | |
| CN108898394A (en) | A kind of safflower DNA Rapid identification Internet of Things anti-fake traceability system and anti-fake source tracing method | |
| Salami et al. | Deep learning image analysis models pretrained on daily objects are useful for the preliminary characterization of particulate pharmaceutical samples | |
| CN113643771A (en) | Medical marketing system based on Taas platform |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181012 |
|
| WW01 | Invention patent application withdrawn after publication |