CN1088976C - Composite diniconazole plant root-growing promoter - Google Patents

Composite diniconazole plant root-growing promoter Download PDF

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Publication number
CN1088976C
CN1088976C CN99121199A CN99121199A CN1088976C CN 1088976 C CN1088976 C CN 1088976C CN 99121199 A CN99121199 A CN 99121199A CN 99121199 A CN99121199 A CN 99121199A CN 1088976 C CN1088976 C CN 1088976C
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root
kcl
potassium chloride
rootage
promoter
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CN99121199A
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CN1268293A (en
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黄桂琴
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Nankai University
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Nankai University
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Abstract

The present invention relates to a promoter for the rootage of plants, which belongs to a plant growth regulator and is prepared by compounding 100 to 1000 ppm of potassium chloride (KCL) and 1 to 10 ppm of uniconazole (S-3307). The promotion functions of the promoter to the rootage and the root growth of cucumber seminal leaves and mung bean hypocotyls are very obvious, synergistic functions are reinforced, and the potassium chloride can induce the S-3307 to increase rootage. Root fresh weight is especially and obviously increased, and the inhibition functions of the S-3307 to root length are reversed. The mixed use of the potassium chloride and the S-3307 can enhance drug effect and reduce the dosage of the S-3307. The present invention has the advantages of remarkably enhanced drug effect, low cost, high safety, reliability and convenient use, and is extensively used for the rootage of plants.

Description

Composite diniconazole plant root-growing promoter
The invention belongs to the plant growth regulator class.
In agricultural production practice, be sought after plant rooting promoter, be mainly used in cuttage.Cuttage has special meaning in the breeding of plant improved seeds, the offspring who cultivates with cottage method could be complete, keeps the good characteristic of parent singlely.The plant seed that has in addition seldom or be difficult to germinate also needs to breed in a large number with cuttage.Root-inducing promoter commonly used at present has heteroauxin (IAA), indolebutyric acid (IBA), and methyl (NAA) etc., but these prices are expensive, use also inconvenient.
Uniconazole P (S-3307) is the efficient plant growth delayer, and its mode of action is mainly synthetic by the inhibition blocking-up GA to the kaurene enzymic activity; Thereby tissue growth is subjected to press down between the envoy, (execute born Lu Dingzhi " S-3307; pp333 is to the comparison of rice seedling root system and overground part growth effect " in suitable concentration range hestening rooting, Plant Physiology Communications 1993,29 (4): 272~274, Duan Rongqi etc. " application of isolated cucumber cotyledon rooting test method " Nankai University's journal, 1998 (3): 109~112), the increase though take root is declared but root is long.
Past, we once found not only the formation and the root growth of plant adventive root are had obvious facilitation as the potassium of one of plant macronutrient, and promote root to extend (Z.R.Zhao especially, G.R.Li and G.Q.Huang " PromotiveEffect of potassium on Adeventitious Root Formation in Some Plants ", Plant Science79 (1991)-47-50; Zhao Zhongren etc. " potassium is taken root to isolated cucumber cotyledon and the facilitation of root growth " Plant Physiology Communications 1991,27 (3): 197~199.
The object of the invention provides a kind of composite diniconazole plant root-growing promoter, potassium chloride and S-3307 are compound, and the number of not only taking root of using with increases, and the root fresh weight especially obviously increases, the adding of potassium chloride can reduce the using dosage of S-3307, thereby reduce cost, potassium chloride and S-3307, the missible oil of S-3307 5% is all water-soluble, and is easy to use.
The present invention is by potassium chloride (KCl) and uniconazole P (S-3307) compound composition, the chemical name of uniconazole P (RS)-E-1-(4-chlorphenyl)-4,4-dimethyl-2-(1,2, the 4-triazol-1-yl)-1-amylene-3 alcohol, general Sumiseven by name.
The common proportioning of the present invention consists of: potassium chloride: uniconazole P=100~1000ppm: 1~10ppm, and the method for preparation is to adopt method in common, the uniconazole P missible oil of use 5% adds the water preparation, adds the aqueous solution of potassium chloride, gets final product.
The present invention is to cucumber cotyledons and mung bean hypocotyl is taken root and the facilitation of root growth is very significant, and synergy is strengthened, and potassium chloride along with the increase of concentration, reverses the long effect of S-3307 inhibition root and obviously strengthens in 100~1000ppm scope.The potassium chloride drug effect that the S-3307 of 1ppm adds 100ppm is better than the S-3307 of 10ppm.The KCl of 100ppm promotes that the mung bean embryo shaddock number of taking root is 25, and S-3307 (effect that the number of taking root of 1~10ppm)+KCl all uses separately greater than KCl, taking root for counting is respectively 37; 46; 44.The heteroauxin of the 10ppm number of taking root is 27, be better than S-3307 (1~10ppm) effect of using separately, but obviously be less than S-3307 and the compound effect of using with of KCl, (1~10ppm)+KCl is taken root and is severally lacked 10 respectively than S-3307; 19; 17.
Drug effect of the present invention significantly improves, and cost is low, and is safe and reliable, easy to use, can be widely used in plant establishment.Following embodiment can demonstrate fully characteristics of the present invention.
Embodiment 1
Adopt the cucumber cotyledons rooting method (with reference to Zhao Shenren, Huang Guiqin, " a kind of new assay method of taking root ", Botany Gazette 1990,32 (10): 815-818), select for use cucumber seeds (Cucumis Sativns L.) to grind No. 4 for Tianjin, soaking seed was sowed on 0.7% agar after 2 hours, and cultivated 72 hours in 26 ℃ of darkrooms, and it is standby to win cotyledon, per 10 cotyledons are placed in the 6cm culture dish, in have a garden filter paper and 3ml to treat test solution, 26 ℃ of darkrooms are cultivated and are measured 10 cotyledons number of taking root, the long and root fresh weight of root after 5 days respectively, test result sees Table 1-4, and the control treatment test result of table 1-3 sees Table 4.
Embodiment 2 adopts the mung bean hypocotyl rooting test methods, with reference to [U.S.] Wei plant growth substance in the work<agricultural not 〉, Science Press, 1979,45-46 page or leaf; Select a mao mung bean (Phaseolus radiatus L.) seed soaking 4 hours for use, be placed on vernalization in the big culture dish of 2 layers of wet filter paper, the planting seed that will show money or valuables one carries unintentionally is on the thin vermiculite of humidity then, 25 ℃ of cultivations, daylight 8 hours (2000Lx) is after 12 days, cut root system, the clip seedling cotyledon saves following 3 centimetres of hypocotyls (comprising epicotyl, first pair of true leaf and trifoliate bud etc.), and it is standby that distilled water is put in segment.Put 50ml in the 50ml beaker and treat test solution, per 10 hypocotyl segments are put into beaker, 25 ℃ of cultivations, daylight 8-10 hour (2000Lx) measures per 10 hypocotyls number of taking root after 6 days, root is long, it is long to calculate take root number and root of average each hypocotyl, the results are shown in Table 5.
Table 1.KCl and S-3307 use the influence that cucumber cotyledons is taken root with
S-3307(ppm) 1ppm 3ppm 10ppm
S-3307 The number of taking root 8.3 9.3 12.3
Promote percentage (%) 31.75 47.62 195.24
S-3307 +100ppm KCl The number of taking root 22.3 26.7 29.3
Promote percentage (%) 253.97 323.81 365.08
S-3307 +300ppm KCl The number of taking root 30.3 31.3 34.6
Promote percentage (%) 380.95 396.82 449.21
S-3307 +500ppm KCl The number of taking root 28.0 34.6 35.0
Promote percentage (%) 344.44 449.21 455.56
S-3307 +1000ppm KCl The number of taking root 32.7 37.0 32.7
Promote percentage (%) 419.05 487.30 419.48
Table 2.KCl and S-3307 use with the take root influence of fresh weight of cucumber cotyledons
S-3307(ppm) 1ppm 3ppm 10ppm
S-3307 Root fresh weight (mg) 2.43 3.17 3.83
Promote percentage (%) -24.06 -0.94 19.96
S-3307 +100ppm KCl Root fresh weight (mg) 15.80 17.83 23.00
Promote percentage (%) 393.75 457.19 618.75
S-3307 +300ppm KCl Root fresh weight (mg) 31.07 32.63 33.00
Promote percentage (%) 868.75 919.69 931.25
S-3307 +500ppm KCl Root fresh weight (mg) 37.23 49.07 43.07
Promote percentage (%) 1063.44 1043.44 1245.94
S-3307 +1000ppm KCl Root fresh weight (mg) 44.70 52.47 36.89
Promote percentage (%) 1296.88 1539.69 1052.19
Table 3.KCl and S-3307 use the influence long to the cucumber cotyledons root with
S-3307(ppm) 1ppm 3ppm 10ppm
S-3307 Root long (mm) 1.27 0.87 0.73
Promote percentage (%) -4.51 -34.59 -45.11
S-3307 +100ppm KCl Root long (mm) 3.40 3.47 3.67
Promote percentage (%) 155.64 157.89 175.94
S-3307 +300ppm KCl Root long (mm) 6.16 4.23 3.10
Promote percentage (%) 363.16 218.05 133.08
S-3307 +500ppm KCl Root long (mm) 6.83 6.10 4.27
Promote percentage (%) 413.53 360.90 221.05
S-3307 +1000ppm KCl Root long (mm) 7.10 6.4 4.17
Promote percentage (%) 433.83 383.46 213.53
The influence of table 4.KCl and IAA cucumber cotyledons root growth
Handle (ppm) The number of taking root Root fresh weight (mg) Root long (mm)
0 6.3 3.2 1.3
KCl 100ppm 12.6 18.4 6.83
300ppm 16.5 22.5 11.44
1000ppm 24.2 29.1 12.66
IAA(10ppm) 20.5 19.6 8.00
Table 5.KCl and S-3307 use the influence that mung bean hypocotyl is taken root with
Handle (ppm) (each hypocotyl) takes root Root length (every hypocotyl, mm)
The number of taking root Promote percentage (%) The root of taking root is long Promote percentage (%)
Contrast 10.0 53.00 3.7
S-3307(1ppm) 15.3 53.00 4.1 10.81
S-3307(3ppm) 18.3 83.00 3.6 -2.7
S-3307(10ppm) 20.0 100.00 2.7 -27.03
1ppm S-3307 +1ppm KCl 37.0 270.00 11.7 216.22
3ppm S-3307 +100ppm KCl 46.0 360.00 9.5 156.76
10ppm S-3307 +100ppm KCl 44.0 340.00 8.4 127.03
10ppm IAA 27.0 177.00 6.8 83.78
100ppm KCl 25.0 150.00 24.5 526.16

Claims (1)

1. a composite diniconazole plant root-growing promoter is characterized in that it by the potassium chloride and the compound aqueous solution of forming of uniconazole P, and the weight ratio of described potassium chloride and uniconazole P is 100~1000ppm: 1~10ppm.
CN99121199A 1999-11-08 1999-11-08 Composite diniconazole plant root-growing promoter Expired - Fee Related CN1088976C (en)

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Publication number Priority date Publication date Assignee Title
CN106258982B (en) * 2016-09-30 2018-01-23 海南波莲水稻基因科技有限公司 A kind of plant establishment strong seedling culture base and its preparation method and application
CN106905037A (en) * 2017-02-24 2017-06-30 黑龙江八农垦大学 A kind of interacting composition for preventing the lodging of mung bean middle and later periods and improving the foliar spray conditioning agent and nutrient of yield

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5461824A (en) * 1992-08-07 1995-10-31 Dowelanco Method for controlling tree growth

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5461824A (en) * 1992-08-07 1995-10-31 Dowelanco Method for controlling tree growth

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* Cited by examiner, † Cited by third party
Title
安徽农业科学第22卷第2期 1994.6.1 烯效唑对水稻生育的调控研究 *

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