CN108850021A - A kind of composition and preparation method thereof for preventing and treating dendrobium candidum soft rot - Google Patents
A kind of composition and preparation method thereof for preventing and treating dendrobium candidum soft rot Download PDFInfo
- Publication number
- CN108850021A CN108850021A CN201810696677.1A CN201810696677A CN108850021A CN 108850021 A CN108850021 A CN 108850021A CN 201810696677 A CN201810696677 A CN 201810696677A CN 108850021 A CN108850021 A CN 108850021A
- Authority
- CN
- China
- Prior art keywords
- dendrobium candidum
- composition
- soft rot
- liquid
- preventing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/04—Pteridophyta [fern allies]; Filicophyta [ferns]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
- A01N65/385—Tobacco
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to pesticide field, in particular to a kind of composition for preventing and treating dendrobium candidum soft rot；Including following raw material by weight：Chinese herbal medicine fluid 30-40, plant extraction liquid 15-25, compound amino acid 2-8, compound bacteria 3-9；The composition of the application preparation effectively inhibits the growth of pathogen, during spraying, the present composition will not damage dendrobium candidum itself, simultaneously, good preventive and therapeutic effect is played to dendrobium candidum soft rot to greatest extent again, to the disease incidence control of the soft rot of vegetables dendrobium candidum 2.26%, to the total effective rate of the dendrobium candidum soft rot fallen ill 90% or more, control efficiency is significant.
Description
Technical field
The present invention relates to pesticide field, in particular to a kind of composition for preventing and treating dendrobium candidum soft rot and its preparation side
Method.
Background technique
Dendrobium candidum, also known as ribbed hedyotis herb are the perennial herbaceous plant that grows nonparasitically upon another plant of orchid family Dendrobium, the stem of dendrobium candidum, because of table
Skin is gained the name in iron green, is a kind of Wild Medicinal of preciousness, is born in the height above sea level rock dark and damp up to 1600 meters of mountainous region half
On, like the environment of warm and moist weather and half yin and half yang, can not resist cold, be generally resistant to -5 DEG C of low temperature, dendrobium nobile can be divided into yellow grass,
Tens of kinds of Jin Chai, horsewhip etc., dendrobium candidum is the superfine product of dendrobium nobile, and dendrobium candidum is had unique medical value, is used as medicine with its stem,
《Chinese Pharmacopoeia》Recording dendrobium nobile has effects that reinforcing stomach reg fluid, nourishing Yin and clearing heat, and Tang opens the classics of Taoist school between the first year《Taoist Scriptures》Once
Dendrobium candidum is classified as " first of Chinese nine big mesonas ", by dendrobium candidum, Herba Saussureae Involueratae, three double ginsengs, 120 years tubers of multiflower knotweed, flowers
The Poria cocos desert cistanche of first, remote mountains ganoderma lucidum, seabed pearl, cordyceps sinensis etc. are classified as Chinese nine big mesonas.
Dendrobium candidum has high medicinal efficacy, 1, the effect of promoting the production of body fluid：With the effect of promoting the production of body fluid, it is mainly shown as promotion body of gland
Secretion and internal organs movement；2, hypoglycemic effect：Dendrobium candidum induces glycosuria blood glucose value to that can reduce streptozotocin；3, enhance body
Immunity：Dendrobium candidum particle can promote the phagocytic function of tumor animal macrophage, enhance the proliferation and differentiation of T lymphocyte
And the activity of NK cell, and the serum hemolysin value of tumor animal can be significantly improved, either to non-specific immune function, or
It is specific cellular immunity and humoral immune function, improve effect.
In view of dendrobium candidum nutritive value abundant and medical value, thus with it is high can implanting, therefore at me
There is plantation in each province of state, and most important dendrobium candidum plantation is disease control, multiple diseases easily occurs in dendrobium candidum plantation, such as soft
Maize ear rot is the Major Diseases of dendrobium candidum, the adjoint miscellaneous bacteria decomposition of protein glue of the soft rot Chang Yin as caused by bacterium generate indoles and
Stench occurs；The soft rot as caused by bread mold has grey black mustiness object on diseased tissues surface, is the sporangiophore and spore of germ
Ascus.Scab slabbing is extended up by petiole, makes leaf-head ecto-entad eventually, is constantly rotted；When being extended up by root, leaf-head
It then rots from inside to outside, is in milk yellow mud shape, has stink, finally lose commodity value.Due to root and the aggrieved rear tissue of stem foot
Become fragile, leaf-head easily falls off, and a touching is fallen, and morbidity advanced stage diseased plant is then voluntarily toppled over.Storage period leaf-head easily comes off or rots, by
Evil leaf vein becomes dark brown.
First with morbidity in front of the onset for soft rot at present, the petiole base and basal part of stem on close ground spray agriculture in time
With 200 mg/litre of streptomysin or new phytomycin, 1000 times of liquid of Dexon powder or 38% phonetic 800 times of liquid of copper bacterium ester of evil frost, or
300 milligrams of 400~600 times of liquid of 50% 600~800 times of ambam liquid or 77% copper hydroxide wettable powder or chloramphenicol/
It rises, sprays 1 time within 7~10 days, totally 2~3 times, severe one progress pouring root treatment, but spraying for traditional medicament, it is residual to there is drug
It staying, causes harm to the human body, the not significant a series of problems of disease-controlling effect, harm is larger, so, it is soft to traditional dendrobium candidum
It is the emphasis studied at present that the prevention and treatment of maize ear rot, which improves,.
Summary of the invention
The present invention in order to solve the above technical problems, provide it is a kind of prevent and treat dendrobium candidum soft rot composition and its preparation
Method.
It is realized particular by following technical scheme：
A kind of composition for preventing and treating dendrobium candidum soft rot, including following raw material by weight：Chinese herbal medicine fluid 30-
40, plant extraction liquid 15-25, compound amino acid 2-8, compound bacteria 3-9.
The Chinese herbal medicine fluid, including following raw material by weight：Lygodium japonicum 15-25, granatum 10-20, the four seasons
Green 12-18, geranium wilfordii 10-20, tribulus terrestris 3-9, cape jasmine 8-12, Radix Ophiopogonis 3-9.
The plant extraction liquid, including following raw material by weight：Tobacco leaf 10-20, radish 3-9, Moringa 10-20,
Pine bark 12-18, jasmine 3-9.
The compound amino acid is alanine, cystine, methionine, two or more mixed in tryptophan
Close object.
The compound bacteria, bacillus licheniformis, colloid bacillus cereus press 0.2-0.6:1 mass ratio is mixed to prepare.
A kind of composition of the prevention and treatment dendrobium candidum soft rot, site of administration be dendrobium nobile root, stem, blade,
It is 1 for dendrobium nobile root administration concentration when application:30-40 is 1 for dendrobium nobile stem administration concentration:40-50, for dendrobium leaf
Face administration concentration is 1:60-80, wherein administration concentration is the quality proportioning of composition and water.
A kind of preparation method of prevention and treatment dendrobium candidum soft rot composition, includes the following steps：
(1) Chinese herbal medicine fluid is prepared：After weighing raw material by Chinese medicine preparation, crushing and water-adding infusion, filtering repeats and adds water infusion
Twice, each 30min mixes the filtrate filtered three times, the ethyl alcohol for mixing 5 times of filtrate quality amounts is added, 38-42KHz's
It is ultrasonically treated 8-12min under frequency, recycles ethyl alcohol, heating is concentrated to give herb liquid and filter residue a；
(2) plant extraction liquid is prepared：Plant material is weighed by formula ratio, is smashed to pieces, the 8-12% of plant material quality is added
Cellulase, 15-25min is patted in rubbing repeatedly, the ethyl alcohol of 5 times of material quality amounts is added, under the frequency of 35-45KHz
It is ultrasonically treated 8-12min, after recycling ethyl alcohol, vegetation water is filtered to obtain, then filter residue is added into water infusion, endures to 1/3 water of amount of water
, filter, gained filtrate mixed with vegetation water again, obtains composite plant liquid and filter residue b；
(3) mixed fermentation：Filter residue a in step (1) and the filter residue b of step (2) is mixed, fermentation liquid is sprayed, ferment 15-
20h is added glucose solution and did not had material, impregnates 30min, filtrate c is recovered by filtration
(4) herb liquid, plant extraction liquid, filtrate c, compound bacteria and compound amino acid are mixed, mixes bottling thoroughly.
The fermentation liquid is by cellulase, saccharomycete by 0.1-0.3:The mass ratio of 0.2-0.4 mixes, and addition is gone
Ionized water is configured to the solution that content is 10-20%.
The fermentation liquid, additive amount are the 15-25% of filter residue a, filter residue b mixing quality.
The fermentation, fermentation temperature are 55-65 DEG C, relative humidity 90-95%.
The control efficiency of the composition of the prevention and treatment dendrobium candidum soft rot of preparation is described further below：
1, several mu of dendrobium candidum planting sites are randomly selected, are randomly divided into 2 groups, are managed using daily management mode,
The difference is that first group in dendrobium candidum plantation, primary composition prepared by the present invention is sprayed to dendrobium candidum weekly,
It is administered respectively according to the administration concentration on root, stem, blade face, second group is prevented and treated referring to first group, to dendrobium candidum
The composition of commercially available prevention and treatment dendrobium candidum soft rot is sprayed, the mu amount of spraying is identical, and test period is entire dendrobium candidum planting season,
The disease incidence of dendrobium candidum soft rot is counted respectively, the results are shown in Table 1：
Table 1
| Project | Average attack rate % |
| First group | 2.26 |
| Second group | 4.65 |
2, several mu of dendrobium candidum planting sites are randomly selected, are randomly divided into 2 groups, class has occurred in every group of plantation dendrobium candidum
Like the soft rot disease of degree, it is managed using daily management mode, the difference is that, first group of iron sheet stone to morbidity
Dry measure used in former times sprays weekly primary composition prepared by the present invention, is administered respectively according to the administration concentration on root, stem, blade face, the
Two groups are treated referring to first group, and the composition of commercially available treatment dendrobium candidum soft rot, the mu amount of spraying are sprayed to dendrobium candidum
It is identical, the treatment condition to dendrobium candidum soft rot is indicated with total effective rate, wherein before the onset of total effective rate representative is restored to
State and the state of an illness be eased, need the summation of the two situation of further medication observation, the results are shown in Table 2：
Table 2
| Project | Total effective rate % |
| First group | 91.5 |
| Second group | 88.3 |
In conclusion the beneficial effects of the present invention are：The combination of dendrobium candidum soft rot is prevented and treated made of Chinese medicine
Object effectively inhibits the growth of pathogen, and during spraying, the present composition will not cause to hurt to dendrobium candidum itself
Evil, meanwhile, and good preventive and therapeutic effect is played to dendrobium candidum soft rot to greatest extent, to the soft rot of vegetables dendrobium candidum
Disease incidence control 2.26%, to the total effective rate of the dendrobium candidum soft rot fallen ill 90% or more, control efficiency
Significantly, effectively contain and killed pathogen, reduce the infringement of pathogen and other pests to vegetables, effectively avoid disease
The growth of opportunistic pathogen ensure that the safety of vegetables.
The Chinese medicine that the present invention uses has the effect of stronger antibacterial bacteriostatic, can effectively play inhibition and kill pathogen
Effect, thus achieve the effect that it is anti-, control and have both, meanwhile, also added in formula improve plant resistance, strengthen immunity
Compound bacteria and compound amino acid, the resistance of plant and the resistance to disease and insect can be effectively improved, soil buffer can be enhanced
Ability is retained moisturizing, enhances crop drought resistance, cold-resistant, waterlogging-resistant ability；Blade protection film can also be strengthened simultaneously, pathogen is resisted and invade
Dye, it is disease-resistant, it is pest-resistant, the disease incidence of dendrobium candidum soft rot is effectively reduced.
Specific embodiment
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited to these realities
Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit
Range.
Embodiment 1
A kind of composition for preventing and treating dendrobium candidum soft rot, including following raw material by weight：Chinese herbal medicine fluid 30kg,
Plant extraction liquid 15kg, compound amino acid 2kg, compound bacteria 3kg；The Chinese herbal medicine fluid, including following original by weight
Material：Lygodium japonicum 15kg, granatum 10kg, Chinese holly 12kg, geranium wilfordii 10kg, tribulus terrestris 3kg, cape jasmine 8kg, Radix Ophiopogonis 3kg；It is described
Plant extraction liquid, including following raw material by weight：Tobacco leaf 10kg, radish 3kg, Moringa 10kg, pine bark 12kg, jasmine
Jasmine 3kg；The compound amino acid is alanine, cystine, methionine, two or more the mixing in tryptophan
Object；The compound bacteria, bacillus licheniformis, colloid bacillus cereus press 0.2:1 mass ratio is mixed to prepare.
A kind of preparation method of prevention and treatment dendrobium candidum soft rot composition, includes the following steps：
(1) Chinese herbal medicine fluid is prepared：After weighing raw material by Chinese medicine preparation, crushing and water-adding infusion, filtering repeats and adds water infusion
Twice, each 30min mixes the filtrate filtered three times, the ethyl alcohol for mixing 5 times of filtrate quality amounts is added, in the frequency of 38KHz
Lower ultrasonic treatment 8min, recycles ethyl alcohol, and heating is concentrated to give herb liquid and filter residue a；
(2) plant extraction liquid is prepared：Plant material is weighed by formula ratio, is smashed to pieces, 8% fibre of plant material quality is added
Plain enzyme is tieed up, 15min is patted in rubbing repeatedly, and the ethyl alcohol of 5 times of amounts of material quality is added, is ultrasonically treated under the frequency of 35KHz
8min filters to obtain vegetation water, then filter residue is added water infusion after recycling ethyl alcohol, endures to 1/3 water of amount of water, filters, will
Gained filtrate mixes with vegetation water again, obtains composite plant liquid and filter residue b；
(3) mixed fermentation：Filter residue a in step (1) and the filter residue b of step (2) is mixed, the 15% of mixing quality is sprayed
Fermentation liquid, at 55 DEG C, relative humidity is fermented 15-20h in the environment of being 90%, and glucose solution is added and did not had material, impregnates
Filtrate c is recovered by filtration in 30min；The fermentation liquid is by cellulase, saccharomycete by 0.1:0.2 mass ratio mixing, adds
Enter deionized water, is configured to the solution that content is 10%；
(4) herb liquid, plant extraction liquid, filtrate c, compound bacteria and compound amino acid are mixed, mixes bottling thoroughly.
Embodiment 2
A kind of composition for preventing and treating dendrobium candidum soft rot, including following raw material by weight：Chinese herbal medicine fluid 40kg,
Plant extraction liquid 25kg, compound amino acid 8kg, compound bacteria 9kg；The Chinese herbal medicine fluid, including following original by weight
Material：Lygodium japonicum 25kg, granatum 20kg, Chinese holly 18kg, geranium wilfordii 20kg, tribulus terrestris 9kg, cape jasmine 12kg, Radix Ophiopogonis 9kg；Institute
The plant extraction liquid stated, including following raw material by weight：Tobacco leaf 20kg, radish 9kg, Moringa 20kg, pine bark 18kg,
Jasmine 9kg；The compound amino acid is alanine, cystine, methionine, two or more mixed in tryptophan
Close object；The compound bacteria, bacillus licheniformis, colloid bacillus cereus press 0.6:1 mass ratio is mixed to prepare.
A kind of preparation method of prevention and treatment dendrobium candidum soft rot composition, includes the following steps：
(1) Chinese herbal medicine fluid is prepared：After weighing raw material by Chinese medicine preparation, crushing and water-adding infusion, filtering repeats and adds water infusion
Twice, each 30min mixes the filtrate filtered three times, the ethyl alcohol for mixing 5 times of filtrate quality amounts is added, in the frequency of 42KHz
Lower ultrasonic treatment 12min, recycles ethyl alcohol, and heating is concentrated to give herb liquid and filter residue a；
(2) plant extraction liquid is prepared：Plant material is weighed by formula ratio, is smashed to pieces, the 12% of plant material quality is added
Cellulase, 25min is patted in rubbing repeatedly, and the ethyl alcohol of 5 times of amounts of material quality is added, is ultrasonically treated under the frequency of 45KHz
12min filters to obtain vegetation water, then filter residue is added water infusion after recycling ethyl alcohol, endures to 1/3 water of amount of water, filtering,
Gained filtrate is mixed with vegetation water again, obtains composite plant liquid and filter residue b；
(3) mixed fermentation：Filter residue a in step (1) and the filter residue b of step (2) is mixed, the 25% of mixing quality is sprayed
Fermentation liquid, at 65 DEG C, relative humidity is fermented 20h in the environment of being 95%, and glucose solution is added and did not had material, impregnates
Filtrate c is recovered by filtration in 30min；The fermentation liquid is by cellulase, saccharomycete by 0.3：0.4 mass ratio mixing, adds
Enter deionized water, is configured to the solution that content is 20%；
(4) herb liquid, plant extraction liquid, filtrate c, compound bacteria and compound amino acid are mixed, mixes bottling thoroughly.
Embodiment 3
A kind of composition for preventing and treating dendrobium candidum soft rot, including following raw material by weight：Chinese herbal medicine fluid 35kg,
Plant extraction liquid 20kg, compound amino acid 5kg, compound bacteria 6kg；The Chinese herbal medicine fluid, including following original by weight
Material：Lygodium japonicum 20kg, granatum 15kg, Chinese holly 15kg, geranium wilfordii 15kg, tribulus terrestris 6kg, cape jasmine 10kg, Radix Ophiopogonis 6kg；Institute
The plant extraction liquid stated, including following raw material by weight：Tobacco leaf 15kg, radish 6kg, Moringa 15kg, pine bark 15kg,
Jasmine 6kg；The compound amino acid is alanine, cystine, methionine, two or more mixed in tryptophan
Close object；The compound bacteria, bacillus licheniformis, colloid bacillus cereus press 0.4:1 mass ratio is mixed to prepare.
A kind of preparation method of prevention and treatment dendrobium candidum soft rot composition, includes the following steps：
(1) Chinese herbal medicine fluid is prepared：After weighing raw material by Chinese medicine preparation, crushing and water-adding infusion, filtering repeats and adds water infusion
Twice, each 30min mixes the filtrate filtered three times, the ethyl alcohol for mixing 5 times of filtrate quality amounts is added, in the frequency of 40KHz
Lower ultrasonic treatment 10min, recycles ethyl alcohol, and heating is concentrated to give herb liquid and filter residue a；
(2) plant extraction liquid is prepared：Plant material is weighed by formula ratio, is smashed to pieces, the fibre of plant material quality 10% is added
Plain enzyme is tieed up, 20min is patted in rubbing repeatedly, and the ethyl alcohol of 5 times of amounts of material quality is added, is ultrasonically treated under the frequency of 40KHz
10min filters to obtain vegetation water, then filter residue is added water infusion after recycling ethyl alcohol, endures to 1/3 water of amount of water, filtering,
Gained filtrate is mixed with vegetation water again, obtains composite plant liquid and filter residue b；
(3) mixed fermentation：Filter residue a in step (1) and the filter residue b of step (2) is mixed, the 20% of mixing quality is sprayed
Fermentation liquid, at 60 DEG C, relative humidity is fermented 18h in the environment of being 93%, and glucose solution is added and did not had material, impregnates
Filtrate c is recovered by filtration in 30min；The fermentation liquid is by cellulase, saccharomycete by 0.2:0.3 mass ratio mixing, adds
Enter deionized water, is configured to the solution that content is 15%；
(4) herb liquid, plant extraction liquid, filtrate c, compound bacteria and compound amino acid are mixed, mixes bottling thoroughly.
Claims (10)
1. a kind of composition for preventing and treating dendrobium candidum soft rot, which is characterized in that including following raw material by weight：Medium-height grass
Medical fluid 30-40, plant extraction liquid 15-25, compound amino acid 2-8, compound bacteria 3-9.
2. a kind of composition for preventing and treating dendrobium candidum soft rot as described in claim 1, which is characterized in that the Chinese herbal medicine
Liquid, including following raw material by weight：Lygodium japonicum 15-25, granatum 10-20, Chinese holly 12-18, geranium wilfordii 10-20,
Tribulus terrestris 3-9, cape jasmine 8-12, Radix Ophiopogonis 3-9.
3. a kind of composition for preventing and treating dendrobium candidum soft rot as described in claim 1, which is characterized in that the plant mentions
Liquid is taken, including following raw material by weight：Tobacco leaf 10-20, radish 3-9, Moringa 10-20, pine bark 12-18, jasmine 3-9.
4. a kind of composition for preventing and treating dendrobium candidum soft rot as described in claim 1, which is characterized in that the compound ammonia
Base acid is alanine, cystine, methionine, two or more the mixture in tryptophan.
5. a kind of composition for preventing and treating dendrobium candidum soft rot as described in claim 1, which is characterized in that described is compound
Bacterium, bacillus licheniformis, colloid bacillus cereus press 0.2-0.6:1 mass ratio is mixed to prepare.
6. a kind of composition for preventing and treating dendrobium candidum soft rot a method as claimed in any one of claims 1 to 5, which is characterized in that application portion
Position is dendrobium nobile root, stem, blade, is 1 for dendrobium nobile root administration concentration in application:30-40 gives for dendrobium nobile stem
Concentration is 1:40-50 is 1 for dendrobium nobile blade face administration concentration:60-80, wherein administration concentration is the quality of composition and water
Proportion.
7. a kind of preparation method for preventing and treating dendrobium candidum soft rot composition as described in claim 1, which is characterized in that including
Following steps：
(1) Chinese herbal medicine fluid is prepared：After weighing raw material by Chinese medicine preparation, crushing and water-adding infusion, filtering repeats and adds water infusion two
Secondary, each 30min mixes the filtrate filtered three times, the ethyl alcohol for mixing 5 times of filtrate quality amounts is added, in the frequency of 38-42KHz
It is ultrasonically treated 8-12min under rate, recycles ethyl alcohol, heating is concentrated to give herb liquid and filter residue a；
(2) plant extraction liquid is prepared：Plant material is weighed by formula ratio, is smashed to pieces, the fibre of the 8-12% of plant material quality is added
Plain enzyme is tieed up, 15-25min is patted in rubbing repeatedly, and the ethyl alcohol of 5 times of amounts of material quality is added, ultrasonic under the frequency of 35-45KHz
8-12min is handled, after recycling ethyl alcohol, vegetation water is filtered to obtain, then filter residue is added into water infusion, endures to 1/3 water of amount of water,
Filtering, gained filtrate is mixed with vegetation water again, obtains composite plant liquid and filter residue b；
(3) mixed fermentation：Filter residue a in step (1) and the filter residue b of step (2) is mixed, fermentation liquid is sprayed, ferment 15-20h,
Glucose solution is added and did not had material, impregnates 30min, filtrate c is recovered by filtration
(4) herb liquid, plant extraction liquid, filtrate c, compound bacteria and compound amino acid are mixed, mixes bottling thoroughly.
8. the preparation method of prevention and treatment dendrobium candidum soft rot composition as claimed in claim 7, which is characterized in that the fermentation
Liquid is by cellulase, saccharomycete by 0.1-0.3:The mass ratio of 0.2-0.4 mixes, and deionized water is added, being configured to content is
The solution of 10-20%.
9. the preparation method of prevention and treatment dendrobium candidum soft rot composition as claimed in claim 7, which is characterized in that the fermentation
Liquid, additive amount are the 15-25% of filter residue a, filter residue b mixing quality.
10. the preparation method of prevention and treatment dendrobium candidum soft rot composition as claimed in claim 7, which is characterized in that the hair
Ferment, fermentation temperature are 55-65 DEG C, relative humidity 90-95%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810696677.1A CN108850021A (en) | 2018-06-29 | 2018-06-29 | A kind of composition and preparation method thereof for preventing and treating dendrobium candidum soft rot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810696677.1A CN108850021A (en) | 2018-06-29 | 2018-06-29 | A kind of composition and preparation method thereof for preventing and treating dendrobium candidum soft rot |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108850021A true CN108850021A (en) | 2018-11-23 |
Family
ID=64297195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810696677.1A Pending CN108850021A (en) | 2018-06-29 | 2018-06-29 | A kind of composition and preparation method thereof for preventing and treating dendrobium candidum soft rot |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108850021A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105475376A (en) * | 2015-11-24 | 2016-04-13 | 广西康寿生物科技有限公司 | Natural plant bactericide for controlling dendrobium insect diseases |
| CN106929447A (en) * | 2017-03-21 | 2017-07-07 | 南京农业大学 | Bacillus licheniformis BL06 and its application |
-
2018
- 2018-06-29 CN CN201810696677.1A patent/CN108850021A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105475376A (en) * | 2015-11-24 | 2016-04-13 | 广西康寿生物科技有限公司 | Natural plant bactericide for controlling dendrobium insect diseases |
| CN106929447A (en) * | 2017-03-21 | 2017-07-07 | 南京农业大学 | Bacillus licheniformis BL06 and its application |
Non-Patent Citations (5)
| Title |
|---|
| 张洪昌 等: "《设施果树高效实用栽培与安全施肥》", 31 January 2017, 中国科学技术出版社 * |
| 杜春梅: "《芽孢杆菌在农业中的研究与应用》", 31 May 2013, 黑龙江大学出版社 * |
| 杨洪强: "《有机园艺》", 31 July 2005, 中国农业出版社 * |
| 汪多仁: "《绿色农药与化肥中间体》", 30 April 2009, 科学技术文献出版社 * |
| 熊辅信 等: "《中药现代研究荟萃》", 31 May 2002, 云南科学技术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106538466B (en) | pig breeding method | |
| KR101359553B1 (en) | A composition promoting the growth of plants and method forpreparing thereof | |
| Pala et al. | Ethnomycological studies of some wild medicinal and edible mushrooms in the Kashmir Himalayas (India) | |
| CN109566671A (en) | A kind of crops Pesticide with Chinese medicinal material and its preparation method and application | |
| CN105613037A (en) | Wild-simulated cultivation method of ganoderma lucidum karst | |
| CN111066831A (en) | Chinese herbal medicine source pesticide | |
| CN109892350A (en) | A kind of composition pesticide of plant source | |
| CN107372653B (en) | Medicament and the application of snail are prevented and treated in officinal dendrobium stem plantation production | |
| CN105272754A (en) | Compound fertilizer special for eggplant growth | |
| CN111110759A (en) | Water-eliminating ointment for treating cancerous ascites and preparation method thereof | |
| CN107365216A (en) | A kind of preventing and treating vegetables, the pesticide made of Chinese medicinal herbs fertilizer and preparation technology of tealeaves pest and disease damage | |
| CN108850021A (en) | A kind of composition and preparation method thereof for preventing and treating dendrobium candidum soft rot | |
| CN105394101A (en) | Pesticide and method for preventing and treating salvia miltiorrhiza continuous cropping withering disease | |
| CN109006317A (en) | A kind of cane planting method | |
| CN107668102A (en) | A kind of composition for preventing and treating broccoli gray mold and preparation method thereof | |
| CN107509686A (en) | A kind of cultural method of arhat chicken | |
| CN108684738A (en) | A kind of composition and preparation method thereof of prevention dendrobium candidum black spot | |
| CN108157402A (en) | A kind of plantation fertilizer Chinese medicine additive and preparation method thereof | |
| CN108812728A (en) | A kind of composition and preparation method thereof for preventing and treating dendrobium candidum anthracnose | |
| JP2001103933A (en) | Mixed food and feed additive each containing spore of bracket fungus of genus fomes | |
| CN106719906A (en) | A kind of Chinese medicine for preventing and treating Chinese yam brown spot and preparation method thereof | |
| CN105851091A (en) | Pollution-free pesticide capable of preventing and treating diseases and insect pests of cabbages | |
| CN108925314A (en) | A method of prevention and treatment dendrobium candidum disease | |
| CN106045735A (en) | Microbial organic fertilizer for preventing and treating peach leaf curl and preparation method thereof | |
| CN106342944A (en) | Chinese herbal medicine control agent for tea diseases and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181123 |