CN108684738A - A kind of composition and preparation method thereof of prevention dendrobium candidum black spot - Google Patents
A kind of composition and preparation method thereof of prevention dendrobium candidum black spot Download PDFInfo
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- CN108684738A CN108684738A CN201810696674.8A CN201810696674A CN108684738A CN 108684738 A CN108684738 A CN 108684738A CN 201810696674 A CN201810696674 A CN 201810696674A CN 108684738 A CN108684738 A CN 108684738A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom not containing sulfur-to-oxygen bonds, e.g. polysulfides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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Abstract
The present invention relates to pesticide field, more particularly to a kind of composition of prevention dendrobium candidum black spot;Including following raw material by weight:Chinese herbal medicine fluid 15-25, compound amino acid 1-3, compound bacteria 1-3;Effectively inhibit the growth of pathogen, during spraying, the present composition will not damage dendrobium candidum itself, simultaneously, good preventive and therapeutic effect is played to dendrobium candidum black spot to greatest extent again, the incidence of the black spot of vegetables dendrobium candidum is controlled 5% or so, to the total effective rate of the dendrobium candidum black spot fallen ill 90% or more, control effect is notable, effectively contain and killed pathogen, the infringement of pathogen and other pests to vegetables is reduced, the growth of pathogen is effectively avoided, ensure that the safety of plant.
Description
Technical field
The present invention relates to pesticide field, more particularly to the composition of a kind of prevention dendrobium candidum black spot and its preparation side
Method.
Background technology
Dendrobium candidum, also known as ribbed hedyotis herb are the perennial herbaceous plant that grows nonparasitically upon another plant of orchid family Dendrobium, the stem of dendrobium candidum, because of table
Skin is gained the name in iron green, is a kind of Wild Medicinal of preciousness, is born in height above sea level up to the dark and damp rock in 1600 meters of mountainous region half
On, the environment of happiness warm and moist weather and half cloudy half light can not resist cold, and generally be resistant to -5 DEG C of low temperature, the stem of noble dendrobium can be divided into yellow grass,
Tens of kinds of Jin Chai, horsewhip etc., dendrobium candidum are the superfine product of the stem of noble dendrobium, and dendrobium candidum is had unique medical value, is used as medicine with its stem,
"Chinese Pharmacopoeia"Recording the stem of noble dendrobium has effects that the classics of Taoist school between the first year are opened in reinforcing stomach reg fluid, nourishing Yin and clearing heat, Tang"Taoist Scriptures"Once
Dendrobium candidum is classified as " first of Chinese nine big celestial grass ", by dendrobium candidum, Herba Saussureae Involueratae, three double ginsengs, 120 years tubers of multiflower knotweed, flowers
The Poria cocos desert cistanche of first, remote mountains ganoderma lucidum, seabed pearl, cordyceps sinensis etc. are classified as Chinese nine big celestial grass.
Dendrobium candidum has a high medicinal efficacy, and 1, the effect of promoting the production of body fluid:With the effect of promoting the production of body fluid, it is mainly shown as promotion body of gland
Secretion and internal organs movement;2, hypoglycemic effect:Dendrobium candidum pair can reduce streptozotocin and induce glycosuria blood glucose value;3, enhance body
Immunity:Dendrobium candidum particle can promote the phagocytic function of tumor animal macrophage, enhance proliferation and the differentiation of T lymphocytes
And the activity of NK cells, and the serum hemolysin value of tumor animal can be significantly improved, either to non-specific immune function, or
It is specific cellular immunity and humoral immune function, improve effect.
In view of the nutritive value and medical value that dendrobium candidum is abundant, thus with it is high can implanting, therefore at me
There is plantation in each province of state, and as Planting Years extend and area expands, various diseases aggravate therewith, Common Diseases have anthracnose,
Epidemic disease, black spot, base rot disease, southern blight etc., wherein black spot incidence is higher, and up to 50-70%, causes iron sheet when serious
Stem of noble dendrobium Severe Reduction.Early-stage study finds that the cause of disease of Yunnan dendrobium candidum black spot is caused to be Alternaria tenuissima (Alternaria
Tenuissima), the bacterium main harm dendrobium candidum young leaves and 2-3 years young leaves extracted out on plant, formed wheel line, invagination it is black
Brown circle or subcircular scab, and the blade around scab can turn yellow, plant leaf all falls off when serious.The disease is not only
The photosynthesis for having seriously affected plant causes the underproduction, while a large amount of toxin generated in the bacterium metabolic process also can be in vivo
Accumulation causes quality to decline, and 50% carbendazim, 1000 times of liquid can be used to spray 2~3 still traditional medicaments for black spot at present
Spray, there is medicament residue, human body damaged, the not notable a series of problems of disease-controlling effect, endanger larger, institute
With it is the emphasis studied at present to be improved to the prevention of traditional dendrobium candidum black spot.
Invention content
The present invention in order to solve the above technical problems, provide it is a kind of prevention dendrobium candidum black spot composition and its preparation
Method.
It is realized particular by following technical scheme:
A kind of composition of prevention dendrobium candidum black spot, including following raw material by weight:Chinese herbal medicine fluid 15-
25, compound amino acid 1-3, compound bacteria 1-3.
The Chinese herbal medicine fluid, including following raw material by weight:Alum 15-25, radix ampelopsis 10-20, Chinese holly 12-
18, Bi Collettii 10-20, tribulus terrestris 12-18, fructus piperis longi 8-12, setose thistle 3-9.
The compound amino acid is alanine, cystine, methionine by 0.2-0.8:0.1-0.3:1 mass ratio is mixed
It closes and is made.
The compound bacteria, bacillus amyloliquefaciens, bacillus laterosporus press 0.1-0.3:1 mass ratio is mixed to prepare.
A kind of composition of the prevention dendrobium candidum black spot, site of administration be stem of noble dendrobium root, stem, blade,
Using when, for stem of noble dendrobium root administration concentration be 1:30-40 is 1 for stem of noble dendrobium stem administration concentration:40-50, for stem of noble dendrobium leaf
Face administration concentration is 1:60-80, wherein administration concentration are the quality proportionings of composition and water.
A kind of preparation method of prevention dendrobium candidum black spot composition, includes the following steps:
(1) it decocts:After weighing raw material by Chinese medicine preparation, crushing and water-adding infusion, filtering repeats and adds water infusion twice, often
Secondary 30min mixes the filtrate filtered three times, and the ethyl alcohol for mixing 5 times of amounts of filtrate quality is added, and is ultrasonically treated, and recycles ethyl alcohol, adds
Thermal concentration obtains liquid and filter residue a;
(2) mixed fermentation:By the filter residue a in step (1), zymotic fluid is sprayed, ferment 15-20h, and glucose solution is added and does not have
Material is crossed, 30min is impregnated, filtrate b is recovered by filtration.
(3) liquid, filtrate b, compound bacteria and compound amino acid are mixed, mixes bottling thoroughly.
The supersound process of the step (1), frequency 45-55KHz, processing time 8-12min.
The zymotic fluid is by lactobacillus plantarum, saccharomycete by 0.1-0.3:The mass ratio of 0.2-0.4 mixes, and is added
Biogas slurry is configured to the solution that content is 10-20%.
The zymotic fluid, additive amount are the 15-25% of filter residue a mass.
The fermentation, fermentation temperature are 55-65 DEG C, relative humidity 90-95%.
The control effect of the composition of the prevention dendrobium candidum black spot of preparation is described further below:
1, several mu of dendrobium candidum planting sites are randomly selected, are randomly divided into 2 groups, are managed using daily management mode,
The difference is that first group dendrobium candidum plantation in, composition prepared by the primary present invention is sprayed to dendrobium candidum weekly,
Dispenser is carried out according to the administration concentration on root, stem, blade face respectively, second group is prevented with reference to first group, to dendrobium candidum
The drug of 201710639738.6 disclosed prevention dendrobium candidum black spots is sprayed, the mu amount of spraying is identical, and test period is entire
Dendrobium candidum planting season respectively counts the incidence of dendrobium candidum black spot, and the results are shown in Table 1:
Table 1
| Project | Average attack rate % |
| First group | 5.12 |
| Second group | 8.65 |
2, several mu of dendrobium candidum planting sites are randomly selected, are randomly divided into 2 groups, class has occurred in every group of plantation dendrobium candidum
Like the black spot disease of degree, it is managed using daily management mode, the difference is that, first group of iron sheet stone to morbidity
Dry measure used in former times sprays weekly composition prepared by the primary present invention, carries out dispenser according to the administration concentration on root, stem, blade face respectively, the
Two groups are treated with reference to first group, and 201710639738.6 disclosed prevention dendrobium candidum black spots are sprayed to dendrobium candidum
Drug, the mu amount of spraying is identical, indicates the treatment to dendrobium candidum black spot with total effective rate, wherein total effective rate generation
Table be restored to before the onset of state and the state of an illness be eased, need the summation of the two situation of further medication observation, as a result such as
Shown in table 2:
Table 2
| Project | Total effective rate % |
| First group | 90.5 |
| Second group | 85.4 |
In conclusion the beneficial effects of the present invention are:The combination of dendrobium candidum black spot is prevented made of Chinese medicine
Object effectively inhibits the growth of pathogen, and during spraying, the present composition will not cause to hinder to dendrobium candidum itself
Evil, meanwhile, and good preventive and therapeutic effect is played to dendrobium candidum black spot to greatest extent, to the black spot of vegetables dendrobium candidum
Incidence control 5% or so, to the total effective rate of the dendrobium candidum black spot fallen ill 90% or more, control effect
Significantly, effectively contain and killed pathogen, reduce the infringement of pathogen and other pests to vegetables, effectively avoid disease
The growth of opportunistic pathogen ensure that the safety of plant.
The Chinese medicine that the present invention uses has the effect of stronger antibacterial bacteriostatic, can effectively play inhibition and kill pathogen
Effect, to achieve the effect that it is anti-, control and have both, meanwhile, also added in formula improve plant resistance, strengthen immunity
Compound bacteria and compound amino acid, the resistance of plant and the resistance to disease and insect can be effectively improved, soil buffer can be enhanced
Ability is retained moisturizing, enhancing crop drought resistance, cold-resistant, waterlogging-resistant ability;Blade protection film can also be strengthened simultaneously, pathogen is resisted and invade
Dye, it is disease-resistant, it is pest-resistant, effectively reduce the incidence of dendrobium candidum black spot.
Specific implementation mode
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited in these realities
Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit
Range.
Embodiment 1
A kind of composition of prevention dendrobium candidum black spot, including following raw material by weight:Chinese herbal medicine fluid 15kg,
Compound amino acid 1kg, compound bacteria 1kg;The Chinese herbal medicine fluid, including following raw material by weight:Alum 15kg, radix ampelopsis
10kg, Chinese holly 12kg, Bi Collettiis 10kg, tribulus terrestris 12kg, fructus piperis longi 8kg, setose thistle 3kg;The compound amino acid is the third ammonia
Acid, cystine, methionine press 0.2:0.1:1 mass ratio is mixed to prepare;The compound bacteria, bacillus amyloliquefaciens, side spore
Bacillus presses 0.1:1 mass ratio is mixed to prepare.
A kind of preparation method of prevention dendrobium candidum black spot composition, includes the following steps:
(1) it decocts:After weighing raw material by Chinese medicine preparation, crushing and water-adding infusion, filtering repeats and adds water infusion twice, often
Secondary 30min mixes the filtrate filtered three times, and the ethyl alcohol for mixing 5 times of amounts of filtrate quality is added, under the frequency of 45KHz, ultrasound
8min is handled, ethyl alcohol is recycled, heating is concentrated to give liquid and filter residue a;
(2) mixed fermentation:By the filter residue a in step (1), 15% zymotic fluid of filter residue a mass is sprayed, at 55 DEG C, phase
Ferment 15h in the environment of being 90% to humidity, and glucose solution is added and did not had material, impregnates 30min, filtrate b is recovered by filtration;Institute
The zymotic fluid stated is by lactobacillus plantarum, saccharomycete by 0.1:0.2 mass ratio mixing, is added biogas slurry, being configured to content is
10% solution.
(3) liquid, filtrate b, compound bacteria and compound amino acid are mixed, mixes bottling thoroughly.
Embodiment 2
A kind of composition of prevention dendrobium candidum black spot, including following raw material by weight:Chinese herbal medicine fluid 25kg,
Compound amino acid 3kg, compound bacteria 3kg;The Chinese herbal medicine fluid, including following raw material by weight:Alum 25kg, radix ampelopsis
20kg, Chinese holly 18kg, Bi Collettiis 20kg, tribulus terrestris 18kg, fructus piperis longi 12kg, setose thistle 9kg;The compound amino acid is the third ammonia
Acid, cystine, methionine press 0.8:0.3:1 mass ratio is mixed to prepare;The compound bacteria, bacillus amyloliquefaciens, side spore
Bacillus presses 0.3:1 mass ratio is mixed to prepare.
A kind of preparation method of prevention dendrobium candidum black spot composition, includes the following steps:
(1) it decocts:After weighing raw material by Chinese medicine preparation, crushing and water-adding infusion, filtering repeats and adds water infusion twice, often
Secondary 30min mixes the filtrate filtered three times, and the ethyl alcohol for mixing 5 times of amounts of filtrate quality is added, under the frequency of 55KHz, ultrasound
12min is handled, ethyl alcohol is recycled, heating is concentrated to give liquid and filter residue a;
(2) mixed fermentation:By the filter residue a in step (1), 25% zymotic fluid of filter residue a mass is sprayed, at 65 DEG C, phase
Ferment 15-20h in the environment of being 95% to humidity, and glucose solution is added and did not had material, impregnates 30min, filtrate is recovered by filtration
b;The zymotic fluid is by lactobacillus plantarum, saccharomycete by 0.3:0.4 mass ratio mixing, is added biogas slurry, is configured to content
For 20% solution.
(3) liquid, filtrate b, compound bacteria and compound amino acid are mixed, mixes bottling thoroughly.
Embodiment 3
A kind of composition of prevention dendrobium candidum black spot, including following raw material by weight:Chinese herbal medicine fluid 20kg,
Compound amino acid 2kg, compound bacteria 2kg;The Chinese herbal medicine fluid, including following raw material by weight:Alum 20kg, radix ampelopsis
15kg, Chinese holly 15kg, Bi Collettiis 15kg, tribulus terrestris 15kg, fructus piperis longi 10kg, setose thistle 6kg;The compound amino acid is the third ammonia
Acid, cystine, methionine press 0.5:0.2:1 mass ratio is mixed to prepare;The compound bacteria, bacillus amyloliquefaciens, side spore
Bacillus presses 0.2:1 mass ratio is mixed to prepare.
A kind of preparation method of prevention dendrobium candidum black spot composition, includes the following steps:
(1) it decocts:After weighing raw material by Chinese medicine preparation, crushing and water-adding infusion, filtering repeats and adds water infusion twice, often
Secondary 30min mixes the filtrate filtered three times, and the ethyl alcohol for mixing 5 times of amounts of filtrate quality is added, under the frequency of 50KHz, ultrasound
10min is handled, ethyl alcohol is recycled, heating is concentrated to give liquid and filter residue a;
(2) mixed fermentation:By the filter residue a in step (1), the zymotic fluid of the 15-25% of filter residue a mass is sprayed, at 60 DEG C,
Relative humidity is fermented 18h in the environment of being 93%, and glucose solution is added and did not had material, impregnates 30min, filtrate b is recovered by filtration;
The zymotic fluid is by lactobacillus plantarum, saccharomycete by 0.2:0.3 mass ratio mixing, is added biogas slurry, being configured to content is
15% solution.
(3) liquid, filtrate b, compound bacteria and compound amino acid are mixed, mixes bottling thoroughly.
Claims (10)
1. a kind of composition of prevention dendrobium candidum black spot, which is characterized in that including following raw material by weight:Medium-height grass
Liquid 15-25, compound amino acid 1-3, compound bacteria 1-3.
2. a kind of composition of prevention dendrobium candidum black spot as described in claim 1, which is characterized in that the Chinese herbal medicine
Liquid, including following raw material by weight:Alum 15-25, radix ampelopsis 10-20, Chinese holly 12-18, Bi Collettiis 10-20, tribulus terrestris
12-18, fructus piperis longi 8-12, setose thistle 3-9.
3. a kind of composition of prevention dendrobium candidum black spot as described in claim 1, which is characterized in that the compound ammonia
Base acid is alanine, cystine, methionine by 0.2-0.8:0.1-0.3:1 mass ratio is mixed to prepare.
4. a kind of composition of prevention dendrobium candidum black spot as described in claim 1, which is characterized in that described is compound
Bacterium, bacillus amyloliquefaciens, bacillus laterosporus press 0.1-0.3:1 mass ratio is mixed to prepare.
5. a kind of composition of prevention dendrobium candidum black spot as described in claim 1-4 is any, which is characterized in that apply portion
Position is stem of noble dendrobium root, stem, blade, is 1 for stem of noble dendrobium root administration concentration in application:30-40 gives for stem of noble dendrobium stem
Concentration is 1:40-50 is 1 for stem of noble dendrobium blade face administration concentration:60-80, wherein administration concentration are the quality of composition and water
Proportioning.
6. a kind of preparation method of prevention dendrobium candidum black spot composition as described in claim 1, which is characterized in that including
Following steps:
(1) it decocts:After weighing raw material by Chinese medicine preparation, crushing and water-adding infusion, filtering repeats and adds water infusion twice, every time
30min mixes the filtrate filtered three times, and the ethyl alcohol for mixing 5 times of amounts of filtrate quality is added, and is ultrasonically treated, and recycles ethyl alcohol, heating
It is concentrated to give liquid and filter residue a;
(2) mixed fermentation:By the filter residue a in step (1), zymotic fluid is sprayed, ferment 15-20h, and glucose solution is added and did not had object
Material impregnates 30min, filtrate b is recovered by filtration;
(3) liquid, filtrate b, compound bacteria and compound amino acid are mixed, mixes bottling thoroughly.
7. the preparation method of prevention dendrobium candidum black spot composition as claimed in claim 6, which is characterized in that the step
(1) supersound process, frequency 45-55KHz, processing time 8-12min.
8. the preparation method of prevention dendrobium candidum black spot composition as claimed in claim 6, which is characterized in that the fermentation
Liquid is by lactobacillus plantarum, saccharomycete by 0.1-0.3:The mass ratio of 0.2-0.4 mixes, and biogas slurry is added, being configured to content is
The solution of 10-20%.
9. the preparation method of prevention dendrobium candidum black spot composition as claimed in claim 6, which is characterized in that the fermentation
Liquid, additive amount are the 15-25% of filter residue a mass.
10. the preparation method of prevention dendrobium candidum black spot composition as claimed in claim 6, which is characterized in that the hair
Ferment, fermentation temperature are 55-65 DEG C, relative humidity 90-95%.
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| CN107846876A (en) * | 2014-12-29 | 2018-03-27 | Fmc有限公司 | Bacillus amyloliquefaciens RTI301 compositions and for beneficial to plant growth and treatment plant disease method |
| CN105594698A (en) * | 2015-12-19 | 2016-05-25 | 佛山市艳晖生物科技有限公司 | Bacillus laterosporus wettable powder preparation method |
| CN106906167A (en) * | 2017-04-26 | 2017-06-30 | 北京农学院 | One plant of biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens and its application |
| CN107251923A (en) * | 2017-07-31 | 2017-10-17 | 贵州盛达生植物发展有限公司 | A kind of medicine for preventing and treating dendrobium candidum black spot and preparation method thereof |
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| Title |
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| 熊辅信 等: "《中药现代研究荟萃》", 31 May 2002, 云南科学技术出版社 * |
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Application publication date: 20181023 |
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