CN108834883A - A method of it is not pinched eggplant clothing tobacco using male sterile line cultivation cigar - Google Patents

A method of it is not pinched eggplant clothing tobacco using male sterile line cultivation cigar Download PDF

Info

Publication number
CN108834883A
CN108834883A CN201810996895.7A CN201810996895A CN108834883A CN 108834883 A CN108834883 A CN 108834883A CN 201810996895 A CN201810996895 A CN 201810996895A CN 108834883 A CN108834883 A CN 108834883A
Authority
CN
China
Prior art keywords
tobacco
cigar
sterile line
eggplant clothing
cultivation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201810996895.7A
Other languages
Chinese (zh)
Other versions
CN108834883B (en
Inventor
阳苇丽
杨兴有
靳冬梅
向杰
周开绪
李爱军
秦艳青
赵宇
李斌
代羽
肖洁
杨吉光
侯涛
曾勇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DAZHOU BRANCH OF SICHUAN TOBACCO Corp
Original Assignee
DAZHOU BRANCH OF SICHUAN TOBACCO Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DAZHOU BRANCH OF SICHUAN TOBACCO Corp filed Critical DAZHOU BRANCH OF SICHUAN TOBACCO Corp
Priority to CN201810996895.7A priority Critical patent/CN108834883B/en
Publication of CN108834883A publication Critical patent/CN108834883A/en
Application granted granted Critical
Publication of CN108834883B publication Critical patent/CN108834883B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/45Tobacco

Abstract

The invention belongs to technical field of tobacco cultivation, disclose it is a kind of do not pinched the method for eggplant clothing tobacco using male sterile line cultivation cigar, comprise the steps of:It is female parent with a certain tobacco male sterile line kind, a cigar eggplant clothing tobacco main breed is male parent, and hybridization obtains F1 generation seed;It is female parent with F1, cigar eggplant clothing tobacco main breed is recurrent parent, and backcrossing obtains BC1 for seed, and 5 generation of continuous backcross obtains BC6 seed, as cigar eggplant clothing male sterile line kind;Transformation eggplant clothing sterile line kind breeds Production of Large Fields seed using former cigar eggplant clothing kind as restorer;The production is not pinched after being buddingged with the cigarette strain that seed is sowed, and priming leaf harvests the system of drying in the air after proper mature (ripe), is fermented after tobacco leaf modulation.It is an advantage of the current invention that the nicotine for the tobacco that this method obtains, nitrogen content and tobacco leaf thickness are lower, in the course of cultivation without pinching and being not easy to lodge, production cost is greatly reduced.

Description

A method of it is not pinched eggplant clothing tobacco using male sterile line cultivation cigar
Technical field
It is specially a kind of not pinched eggplant using male sterile line cultivation cigar the invention belongs to tobacco planting technical field The method of clothing tobacco.
Background technique
Cigar is a kind of important tobacco category, rolls cigar and needs three kinds of eggplant core, eggplant set, eggplant clothing raw tobacco materials, Middle eggplant clothing is the essential part of cigar and the part of most expensive, quality of tobacco is required it is high, the cigar of domestic production Eggplant clothing relies primarily on import.The production pilot work of cigar eggplant clothing raw tobacco material has been carried out in Some Domestic producing region at present, achieves Some progress.For example, a kind of for avenging dedicated tobacco leaf production method (the Chinese invention patent publication No. of refined fragrant cigarette product CN104687232A):Make stock with cigar tobacco seedlings, the two is grafted onto together as scion, carries out production cultivation by cured tobacco seedling, Gained grafts tobacco seedlings and carries out field planting, and gained raw tobacco material product, bright characteristic is best in quality, can satisfy the refined fragrant product of snow Demand of the class cigarette product to raw material;A kind of cultural method of cigar wrapper tobacco leaves (Chinese invention patent publication No. CN101946622A):Seed sowing-tobacco seedlings transplanting-when tobacco seedlings are grown to carries out pinching when floral axis goes out-after tobacco leaf proper mature (ripe) by position Harvesting, the tobacco leaf that gained tobacco leaf cigarette face is open and flat, thickness is moderate, tendon and vessel is tiny, damaged degree is small, color is deeper meet high-grade cigar The requirement of wrapper tobacco leaves.Although both methods can produce the satisfactory eggplant clothing of a generation, can not obtain meeting item for a long time The eggplant clothing of part.
For the cultivating and growing of eggplant clothing tobacco leaf, external high-quality eggplant clothing tobacco leaf generally takes cultivation of not pinching, due to not pinching, It is easy to cause cigarette strain to lodge after production later period seed-setting, people take cigarette strain hack lever or tied rope to hang fixed method solution Certainly, but this mode recruitment cost and goods and materials higher cost.The country mostly uses topping cultivation (bud is won after occurring), this planting Training mode causes tobacco leaf to there is a problem of that nicotine content is high, blade is thick, tissue is not fine and smooth.
Currently, a kind of cultural method can produce while overcome the plant of the above problem not yet.Therefore, it is badly in need of developing one kind Exempt from the cultural method of topping and non-lodging.
Summary of the invention
In order to solve to need to pinch in above-mentioned cultivation of the existing technology or need additional means to resistant to lodging Problem is not pinched the method for eggplant clothing tobacco it is an object of that present invention to provide a kind of using male sterile line cultivation cigar, this hair The bright application additionally provided using the obtained tobacco leaf of preceding method and this method.
Present invention seek to address that needing to take cultivation step of not pinching in the production of eggplant clothing, to reduce vane thickness, reduce Nicotine content increases flexibility, eggplant clothing quality of tobacco can be improved, while solution never pinches and cultivates the easy lodging of cigarette strain in production Problem solves the problems, such as that the cost of traditional lodging-prevention method is excessively high.
Tobacco male sterile line is usually to be used as tobacco bred breeding, is applied to production for the breeding first generation of hybrid, or Conventional kind of transformation is sterile line, after the cultivation of these male sterile line tobacco breds all to protect variety source by breeding units It will do it topping, not yet find to exempt from culture and utility of pinching using tobacco male sterile line at present.
The technical scheme adopted by the invention is as follows:
A method of it is not pinched eggplant clothing tobacco, is comprised the steps of using male sterile line cultivation cigar:
It (1) is female parent with tobacco male sterile line kind, cigar eggplant clothing tobacco main breed is male parent, and hybridization obtains F1 Seed;
It (2) is female parent with F1, cigar eggplant clothing tobacco main breed is recurrent parent, and backcrossing obtains BC1 seed, then continuously It was returned for 5 generations, obtains BC6 seed, as cigar eggplant clothing male sterile line kind;
(3) using cigar eggplant clothing tobacco main breed used in step (1) as restorer, cigar sterile line product are bred Kind production seed;
(4) cigarette strain that the cigar eggplant clothing sterile line variety seeds are sowed is not pinched after buddingging, priming leaf after proper mature (ripe) The system of drying in the air is harvested, is fermented after tobacco leaf modulation.
Specifically, the cigar eggplant clothing tobacco main breed includes CX1 and Havana92 in the above method.
Specifically, the tobacco male sterile line kind is cultivation safflower tobacco sterile line in the above method.
Specifically, the tobacco male sterile line kind includes MSKY14 and MS Yun yan85 in the above method.
The present invention also provides a kind of tobacco leaves as made from preceding method.
Specifically, above-mentioned tobacco leaf, the nicotine content of the tobacco leaf of the cigar eggplant clothing male sterile line kind is relative to original The nicotine content for the tobacco leaf that the topping cultivation of cigar eggplant clothing tobacco main breed obtains reduces by 50% or more.
Specifically, above-mentioned tobacco leaf, the total nitrogen content of the tobacco leaf of the cigar eggplant clothing male sterile line kind is relative to original The total nitrogen content for the tobacco leaf that the topping cultivation of cigar eggplant clothing tobacco main breed obtains reduces by 50% or more.
Specifically, above-mentioned tobacco leaf, the vane thickness of the tobacco leaf of the cigar eggplant clothing male sterile line kind is relative to original The vane thickness for the tobacco leaf that the topping cultivation of cigar eggplant clothing tobacco main breed obtains reduces by 50% or more.
The present invention also provides a kind of application of preceding method in cigar tobacco cultivation.
Beneficial effects of the present invention are:
It is provided by the invention not pinched the method for eggplant clothing tobacco using male sterile line cultivation cigar, it can be effectively reduced Nicotine, total nitrogen content and the tobacco leaf thickness of the tobacco leaf of acquisition produce more good tobacco leaf, improve the product of cigar obtained Matter.Meanwhile the tobacco that this method obtains is not necessarily to pinch and will not lodge in the course of cultivation, it, can without measure resistant to lodging 15/hm2 or more of production labor is reduced, production cost is greatly reduced.
Specific embodiment
Further explaination is done to the present invention combined with specific embodiments below.
Embodiment 1
It a kind of is not pinched the side of eggplant clothing tobacco using male sterile line cultivation cigar the purpose of the present embodiment is that providing Method, the specific method is as follows:
The first step:Excellent cigar eggplant clothing tobacco main breed CX1 is selected, male sterile line kind MSKY14 is selected, It is maternal, CX1 as male parent using sterile line MSKY14, hybridization obtains the (i.e. MSKY14 × CX1 hybridization of F1 (MSKY14 × CX1) seed The seed of acquisition, similarly hereinafter).
Second step:It is female parent with F1 (MSKY14 × CX1), eggplant clothing kind CX1 is recurrent parent, and backcrossing obtains BC1 seed; Using eggplant clothing kind CX1 as recurrent parent, and backcross progeny 5 generation of continuous backcross again, until obtaining BC6 seed, as cigar eggplant The sterile line new varieties MSCX1 of clothing kind CX1.
Third step:It is restorer with eggplant clothing kind CX1, MSCX1 is sterile line, breeds the seed of MSCX1 production.
Third step:Cultivation of not pinching according to cigar tobacco produces routine techniques, by cigar eggplant clothing male sterile line MSCX1 Sowing, nursery, transplanting, fertilising, field management.Cigarette strain is not required to pinch after buddingging, and after proper mature (ripe), the harvesting of tobacco leaf Divisional priming leaf is dried in the air System.
Effect example 1
By MSCX1, CX1 according to the same terms growing and cultivation, three groups are set, MSCX1 is processing group, the topping of CX1 kind Cultivation be control 1, CX1 do not pinch cultivation be compare 2.Hack lever tied rope lodging-prevention is applied to control 2, other measures are identical.Compare Quality of tobacco, the production labor cost of MSCX1 and control group that method provided by the invention obtains.Specific comparative situation is shown in Table 1 With table 2.
Table 1
Table 2
Processing The amount of labour used/hm2 Goods and materials cost (member/hm2) Totle drilling cost (member/hm2)
Processing 0 0 0
Control 1 15 0 1500
Control 2 45 1500 6000
As can be seen from Table 1, relative to processing group (MSCX1), the nicotine of the tobacco leaf (control 1) of the plant for cultivation of pinching contains Amount increases by 135%, and total nitrogen increases by 82.5%, and vane thickness increases by 61.7%, i.e., method provided by the invention can be effectively reduced Tobacco leaf nicotine, nitrogen content, tobacco leaf thickness reduce.It is well known that nicotine, nitrogen content and tobacco leaf thickness are to directly affect tobacco leaf product Matter determines that the important indicator of quality of tobacco, method provided by the invention can produce more good tobacco leaf compared with the existing technology. As shown in Table 2, relatively conventional cultivation of not pinching, the MSCX1 that method provided by the invention obtains pinch and will not lodge due to being not necessarily to, Totle drilling cost is zero, greatly reduces the topping or the expenditure to project resistant to lodging in cultivation, substantially reduces production cost.
Embodiment 2
As in the first embodiment, implementing this method using other kinds, the specific method is as follows:
The first step:Excellent cigar eggplant clothing tobacco main breed Havana92 is selected, male sterile line kind MS is selected Yun yan85 is maternal, Havana92 as male parent using sterile line MS Yun yan85, and hybridization obtains F1 (MS Yun yan85 × Havana92) kind Son.
Second step:It is female parent with F1 (MS Yun yan85 × Havana92), cigar eggplant clothing kind Havana92 is circulation parent This, backcrossing obtains BC1 seed;5 generation of continuous backcross again, until obtaining BC6 seed, as cigar eggplant clothing kind Havana92's Sterile line new varieties MS Havana92.
Third step:It is restorer with eggplant clothing kind Havana92, MS Havana92 is sterile line, breeds MS Havana92 The seed of production.
Third step:Cultivation of not pinching according to cigar tobacco produces routine techniques, by cigar eggplant clothing male sterile line MS The sowing of Havana92 seed, nursery, transplanting, fertilising, field management.Cigarette strain is not required to pinch after buddingging, after proper mature (ripe), by tobacco leaf branch Position priming leaf harvests the system of drying in the air.
Effect example 2
By MS Havana92, conventional H avana92 according to the same terms growing and cultivation, three groups, MS Havana92 are set Cultivation do not pinch as processing group, conventional H avana92 kind pinches cultivation as control 1, and it is pair that conventional H avana92, which does not pinch and cultivates, According to 2.2 application hack lever tied rope lodging-prevention of control, other measures are identical.The MS that method more provided by the invention obtains The quality of tobacco of Havana92 and control group, topping and lodging-prevention production labor cost.Specific comparative situation is shown in Tables 1 and 2.
Table 3
Table 4
Processing The amount of labour used/hm2 Goods and materials cost (member/hm2) Totle drilling cost (member/hm2)
Processing 0 0 0
Control 1 15 0 1500
Control 2 45 1500 6000
As can be seen from Table 3, relative to processing group (MS Havana92 does not pinch), the tobacco leaf of the plant for cultivation of pinching is (right Increase according to nicotine, total nitrogen 1), vane thickness increases, i.e., tobacco leaf nicotine can be effectively reduced in method provided by the invention, nitrogen contains Amount, tobacco leaf thickness reduce.As shown in Table 4, relatively conventional cultivation of not pinching, the technology that method provided by the invention obtains is due to being not necessarily to It topping and will not lodge, this work totle drilling cost is zero, greatly reduces in cultivation the topping or to project resistant to lodging Cost.
Contrast table 1 and table 3, table 2 and table 4 are it is found that nicotine of tobacco leaf of processing group etc. contains in embodiment 1 and embodiment 2 Amount is greatly lowered, and the reduction amplitude of amount of labour used etc. is larger.It is only exemplified by above-mentioned two embodiment as space is limited, but From in the actual application of above-described embodiment and the method for offer of the invention it is found that method provided by the invention can be used for A variety of male sterile lines cultivation cigars are not pinched eggplant clothing tobacco, however it is not limited to a certain kind.
The present invention is not limited to above-mentioned optional embodiment, anyone can show that other are each under the inspiration of the present invention The product of kind form.Above-mentioned specific embodiment should not be understood the limitation of pairs of protection scope of the present invention, protection of the invention Range should be subject to be defined in claims, and specification can be used for interpreting the claims.

Claims (9)

1. a kind of do not pinched the method for eggplant clothing tobacco using male sterile line cultivation cigar, which is characterized in that include following step Suddenly:
It (1) is female parent with tobacco male sterile line kind, cigar eggplant clothing tobacco main breed is male parent, and hybridization obtains F1 generation kind Son;
It (2) is female parent with F1 generation, cigar eggplant clothing tobacco main breed is recurrent parent, and backcrossing obtains BC1 seed, then continuously returns It handed over for 5 generations, obtains BC6 seed, as cigar eggplant clothing male sterile line kind;
(3) using cigar eggplant clothing tobacco main breed used in step (1) as restorer, breeding cigar sterile line kind is raw Production seed;
(4) cigarette strain that the cigar eggplant clothing sterile line variety seeds are sowed is not pinched after buddingging, and priming leaf harvests after proper mature (ripe) The system of drying in the air is fermented after tobacco leaf modulation.
2. according to the method described in claim 1, it is characterized in that:The cigar eggplant clothing tobacco main breed include CX1 and Havana92。
3. according to the method described in claim 2, it is characterized in that:The tobacco male sterile line kind is cultivation safflower tobacco Sterile line.
4. according to the method described in claim 3, it is characterized in that:The tobacco male sterile line kind includes MSKY14 and MS Yun yan85.
5. a kind of tobacco leaf as made from method as claimed in claim 4.
6. tobacco leaf according to claim 5, it is characterised in that:The tobacco leaf of the cigar eggplant clothing male sterile line kind Nicotine content relative to the nicotine content of tobacco leaf that the topping cultivation of former cigar eggplant clothing tobacco main breed obtains reduce by 50% with On.
7. tobacco leaf according to claim 5, it is characterised in that:The tobacco leaf of the cigar eggplant clothing male sterile line kind Total nitrogen content relative to the total nitrogen content of tobacco leaf that the topping cultivation of former cigar eggplant clothing tobacco main breed obtains reduce by 50% with On.
8. tobacco leaf according to claim 5, it is characterised in that:The tobacco leaf of the cigar eggplant clothing male sterile line kind Vane thickness relative to the vane thickness of tobacco leaf that the topping cultivation of former cigar eggplant clothing tobacco main breed obtains reduce by 50% with On.
9. application of the method in cigar tobacco cultivation described in a kind of claim 1-4 any one.
CN201810996895.7A 2018-08-29 2018-08-29 Method for cultivating cigar tobacco without topping by utilizing male sterile line Active CN108834883B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810996895.7A CN108834883B (en) 2018-08-29 2018-08-29 Method for cultivating cigar tobacco without topping by utilizing male sterile line

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810996895.7A CN108834883B (en) 2018-08-29 2018-08-29 Method for cultivating cigar tobacco without topping by utilizing male sterile line

Publications (2)

Publication Number Publication Date
CN108834883A true CN108834883A (en) 2018-11-20
CN108834883B CN108834883B (en) 2020-04-28

Family

ID=64233837

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810996895.7A Active CN108834883B (en) 2018-08-29 2018-08-29 Method for cultivating cigar tobacco without topping by utilizing male sterile line

Country Status (1)

Country Link
CN (1) CN108834883B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110692478A (en) * 2019-11-13 2020-01-17 四川省烟草公司达州市公司 Cigar tobacco cultivation method for one-plant production of eggplant-coated tobacco leaves and eggplant-cored tobacco leaves

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090117212A1 (en) * 2007-11-06 2009-05-07 Alliance One International, Inc. Tobacco cultivar aob 176 and products therefrom
US20090119806A1 (en) * 2007-11-06 2009-05-07 Alliance One International, Inc. Tobacco cultivar aob 175 and products therefrom
CN101946622A (en) * 2010-08-31 2011-01-19 海南建恒哈瓦那雪茄有限公司 Method for cultivating cigar wrapper tobacco leaves
CN104687232A (en) * 2015-01-15 2015-06-10 湖北中烟工业有限责任公司 Special tobacco leaf production method for Xueyaxiang cigarette products
CN105123305A (en) * 2015-08-06 2015-12-09 中国烟草总公司福建省公司 Flue-cured tobacco nicotine content reducing method
CN105145348A (en) * 2015-11-02 2015-12-16 贵州省烟草公司遵义市公司 Breeding and propagation method of sterile line novel variety of flue-cured tobacco
CN105230476A (en) * 2015-10-29 2016-01-13 湖北省烟草科学研究院 Breeding method for male sterile hybrid of low-nicotine burley tobacco
CN105686053A (en) * 2016-03-15 2016-06-22 河南中烟工业有限责任公司 Method for manufacturing coats and covers of cigars
CN107466840A (en) * 2017-09-05 2017-12-15 湖南省烟草公司郴州市公司 A kind of producing method for seed of flue-cured tobacco male sterile line kind

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090117212A1 (en) * 2007-11-06 2009-05-07 Alliance One International, Inc. Tobacco cultivar aob 176 and products therefrom
US20090119806A1 (en) * 2007-11-06 2009-05-07 Alliance One International, Inc. Tobacco cultivar aob 175 and products therefrom
CN101946622A (en) * 2010-08-31 2011-01-19 海南建恒哈瓦那雪茄有限公司 Method for cultivating cigar wrapper tobacco leaves
CN104687232A (en) * 2015-01-15 2015-06-10 湖北中烟工业有限责任公司 Special tobacco leaf production method for Xueyaxiang cigarette products
CN105123305A (en) * 2015-08-06 2015-12-09 中国烟草总公司福建省公司 Flue-cured tobacco nicotine content reducing method
CN105230476A (en) * 2015-10-29 2016-01-13 湖北省烟草科学研究院 Breeding method for male sterile hybrid of low-nicotine burley tobacco
CN105145348A (en) * 2015-11-02 2015-12-16 贵州省烟草公司遵义市公司 Breeding and propagation method of sterile line novel variety of flue-cured tobacco
CN105686053A (en) * 2016-03-15 2016-06-22 河南中烟工业有限责任公司 Method for manufacturing coats and covers of cigars
CN107466840A (en) * 2017-09-05 2017-12-15 湖南省烟草公司郴州市公司 A kind of producing method for seed of flue-cured tobacco male sterile line kind

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
时向东等: "栽培密度对雪茄外包皮烟叶片化学成分和物理特性的影响", 《中国烟草学报》 *
李浩等: "优质丰产白肋烟品种达白1号的选育与应用", 《贵州农业科学》 *
秦艳青等: "优质雪茄茄衣生产技术探讨", 《江西农业学报》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110692478A (en) * 2019-11-13 2020-01-17 四川省烟草公司达州市公司 Cigar tobacco cultivation method for one-plant production of eggplant-coated tobacco leaves and eggplant-cored tobacco leaves

Also Published As

Publication number Publication date
CN108834883B (en) 2020-04-28

Similar Documents

Publication Publication Date Title
Ercan et al. Influence of growing season and donor plant age on anther culture response of some pepper cultivars (Capsicum annuum L.)
CHIANG SHIONG et al. Production of haploid plants from anther cultures and secondary embryoids of winter oilseed rape, Brassica napus ssp. oleifera
CN106489570B (en) A kind of cigarette strain breeding method improving the early blossoming tobacco field output value
CN109220793B (en) Breeding method of new butterfly orchid variety
CN101019505A (en) Breeding process of rice blast-resisting high quality and high yield round-grained rice variety Zhendao-10
CN104429932A (en) Novel indica-japonica hybrid gene introgression sheath blight resistance breeding method
CN106258937B (en) A kind of quickly breeding is disease-resistant, the method for good quality wheat strain
CN110679478A (en) Breeding method of forage grass type BMR sorghum sterile line
CN114041419A (en) Breeding method of new high-quality rice variety with false smut resistance
CN108834883A (en) A method of it is not pinched eggplant clothing tobacco using male sterile line cultivation cigar
CN110100672B (en) Method for rapidly producing rice photo-thermo-sensitive sterile line stock
CN107347625B (en) Strong application of the female self-mating system LC-03 in pumpkin breeding
CN101810136A (en) Cauliflower cytoplasmic male sterile line breeding method and application of male sterile line
CN103190336A (en) Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids
CN104351000A (en) Culture method of pomelo hybrid
CN109601370B (en) Cascade selection method suitable for breeding new tobacco strain
Mattingly et al. The use of anther-derived haploids in Nicotiana: III. Isolation of nullisomics from monosomic lines
CN110100721A (en) A kind of purplish red leaf sightseeing type multipurpose cabbage type rape selection
CN114532225B (en) Tissue culture rapid propagation and cultivation method for paphiopedilum delbrueckii
CN105075854A (en) Breeding method for early-maturing and disease-resistant broccoli
CN105918109B (en) A kind of fast dish selection of purple
CN112616651B (en) Breeding method of glyphosate-resistant cotton genic male sterile dual-purpose line
CN109105257B (en) Method for tissue culture and rapid propagation of flue-cured tobacco seedlings
CN108552047B (en) Breeding method of flue-cured tobacco with high fragrance and efficient utilization of nitrogen
CN104472060A (en) Method for increasing germination rate of flemingiaphilippinensis seeds

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant