CN108834883A - A method of it is not pinched eggplant clothing tobacco using male sterile line cultivation cigar - Google Patents
A method of it is not pinched eggplant clothing tobacco using male sterile line cultivation cigar Download PDFInfo
- Publication number
- CN108834883A CN108834883A CN201810996895.7A CN201810996895A CN108834883A CN 108834883 A CN108834883 A CN 108834883A CN 201810996895 A CN201810996895 A CN 201810996895A CN 108834883 A CN108834883 A CN 108834883A
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- China
- Prior art keywords
- tobacco
- cigar
- sterile line
- eggplant clothing
- cultivation
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
Abstract
The invention belongs to technical field of tobacco cultivation, disclose it is a kind of do not pinched the method for eggplant clothing tobacco using male sterile line cultivation cigar, comprise the steps of:It is female parent with a certain tobacco male sterile line kind, a cigar eggplant clothing tobacco main breed is male parent, and hybridization obtains F1 generation seed;It is female parent with F1, cigar eggplant clothing tobacco main breed is recurrent parent, and backcrossing obtains BC1 for seed, and 5 generation of continuous backcross obtains BC6 seed, as cigar eggplant clothing male sterile line kind;Transformation eggplant clothing sterile line kind breeds Production of Large Fields seed using former cigar eggplant clothing kind as restorer;The production is not pinched after being buddingged with the cigarette strain that seed is sowed, and priming leaf harvests the system of drying in the air after proper mature (ripe), is fermented after tobacco leaf modulation.It is an advantage of the current invention that the nicotine for the tobacco that this method obtains, nitrogen content and tobacco leaf thickness are lower, in the course of cultivation without pinching and being not easy to lodge, production cost is greatly reduced.
Description
Technical field
It is specially a kind of not pinched eggplant using male sterile line cultivation cigar the invention belongs to tobacco planting technical field
The method of clothing tobacco.
Background technique
Cigar is a kind of important tobacco category, rolls cigar and needs three kinds of eggplant core, eggplant set, eggplant clothing raw tobacco materials,
Middle eggplant clothing is the essential part of cigar and the part of most expensive, quality of tobacco is required it is high, the cigar of domestic production
Eggplant clothing relies primarily on import.The production pilot work of cigar eggplant clothing raw tobacco material has been carried out in Some Domestic producing region at present, achieves
Some progress.For example, a kind of for avenging dedicated tobacco leaf production method (the Chinese invention patent publication No. of refined fragrant cigarette product
CN104687232A):Make stock with cigar tobacco seedlings, the two is grafted onto together as scion, carries out production cultivation by cured tobacco seedling,
Gained grafts tobacco seedlings and carries out field planting, and gained raw tobacco material product, bright characteristic is best in quality, can satisfy the refined fragrant product of snow
Demand of the class cigarette product to raw material;A kind of cultural method of cigar wrapper tobacco leaves (Chinese invention patent publication No.
CN101946622A):Seed sowing-tobacco seedlings transplanting-when tobacco seedlings are grown to carries out pinching when floral axis goes out-after tobacco leaf proper mature (ripe) by position
Harvesting, the tobacco leaf that gained tobacco leaf cigarette face is open and flat, thickness is moderate, tendon and vessel is tiny, damaged degree is small, color is deeper meet high-grade cigar
The requirement of wrapper tobacco leaves.Although both methods can produce the satisfactory eggplant clothing of a generation, can not obtain meeting item for a long time
The eggplant clothing of part.
For the cultivating and growing of eggplant clothing tobacco leaf, external high-quality eggplant clothing tobacco leaf generally takes cultivation of not pinching, due to not pinching,
It is easy to cause cigarette strain to lodge after production later period seed-setting, people take cigarette strain hack lever or tied rope to hang fixed method solution
Certainly, but this mode recruitment cost and goods and materials higher cost.The country mostly uses topping cultivation (bud is won after occurring), this planting
Training mode causes tobacco leaf to there is a problem of that nicotine content is high, blade is thick, tissue is not fine and smooth.
Currently, a kind of cultural method can produce while overcome the plant of the above problem not yet.Therefore, it is badly in need of developing one kind
Exempt from the cultural method of topping and non-lodging.
Summary of the invention
In order to solve to need to pinch in above-mentioned cultivation of the existing technology or need additional means to resistant to lodging
Problem is not pinched the method for eggplant clothing tobacco it is an object of that present invention to provide a kind of using male sterile line cultivation cigar, this hair
The bright application additionally provided using the obtained tobacco leaf of preceding method and this method.
Present invention seek to address that needing to take cultivation step of not pinching in the production of eggplant clothing, to reduce vane thickness, reduce
Nicotine content increases flexibility, eggplant clothing quality of tobacco can be improved, while solution never pinches and cultivates the easy lodging of cigarette strain in production
Problem solves the problems, such as that the cost of traditional lodging-prevention method is excessively high.
Tobacco male sterile line is usually to be used as tobacco bred breeding, is applied to production for the breeding first generation of hybrid, or
Conventional kind of transformation is sterile line, after the cultivation of these male sterile line tobacco breds all to protect variety source by breeding units
It will do it topping, not yet find to exempt from culture and utility of pinching using tobacco male sterile line at present.
The technical scheme adopted by the invention is as follows:
A method of it is not pinched eggplant clothing tobacco, is comprised the steps of using male sterile line cultivation cigar:
It (1) is female parent with tobacco male sterile line kind, cigar eggplant clothing tobacco main breed is male parent, and hybridization obtains F1
Seed;
It (2) is female parent with F1, cigar eggplant clothing tobacco main breed is recurrent parent, and backcrossing obtains BC1 seed, then continuously
It was returned for 5 generations, obtains BC6 seed, as cigar eggplant clothing male sterile line kind;
(3) using cigar eggplant clothing tobacco main breed used in step (1) as restorer, cigar sterile line product are bred
Kind production seed;
(4) cigarette strain that the cigar eggplant clothing sterile line variety seeds are sowed is not pinched after buddingging, priming leaf after proper mature (ripe)
The system of drying in the air is harvested, is fermented after tobacco leaf modulation.
Specifically, the cigar eggplant clothing tobacco main breed includes CX1 and Havana92 in the above method.
Specifically, the tobacco male sterile line kind is cultivation safflower tobacco sterile line in the above method.
Specifically, the tobacco male sterile line kind includes MSKY14 and MS Yun yan85 in the above method.
The present invention also provides a kind of tobacco leaves as made from preceding method.
Specifically, above-mentioned tobacco leaf, the nicotine content of the tobacco leaf of the cigar eggplant clothing male sterile line kind is relative to original
The nicotine content for the tobacco leaf that the topping cultivation of cigar eggplant clothing tobacco main breed obtains reduces by 50% or more.
Specifically, above-mentioned tobacco leaf, the total nitrogen content of the tobacco leaf of the cigar eggplant clothing male sterile line kind is relative to original
The total nitrogen content for the tobacco leaf that the topping cultivation of cigar eggplant clothing tobacco main breed obtains reduces by 50% or more.
Specifically, above-mentioned tobacco leaf, the vane thickness of the tobacco leaf of the cigar eggplant clothing male sterile line kind is relative to original
The vane thickness for the tobacco leaf that the topping cultivation of cigar eggplant clothing tobacco main breed obtains reduces by 50% or more.
The present invention also provides a kind of application of preceding method in cigar tobacco cultivation.
Beneficial effects of the present invention are:
It is provided by the invention not pinched the method for eggplant clothing tobacco using male sterile line cultivation cigar, it can be effectively reduced
Nicotine, total nitrogen content and the tobacco leaf thickness of the tobacco leaf of acquisition produce more good tobacco leaf, improve the product of cigar obtained
Matter.Meanwhile the tobacco that this method obtains is not necessarily to pinch and will not lodge in the course of cultivation, it, can without measure resistant to lodging
15/hm2 or more of production labor is reduced, production cost is greatly reduced.
Specific embodiment
Further explaination is done to the present invention combined with specific embodiments below.
Embodiment 1
It a kind of is not pinched the side of eggplant clothing tobacco using male sterile line cultivation cigar the purpose of the present embodiment is that providing
Method, the specific method is as follows:
The first step:Excellent cigar eggplant clothing tobacco main breed CX1 is selected, male sterile line kind MSKY14 is selected,
It is maternal, CX1 as male parent using sterile line MSKY14, hybridization obtains the (i.e. MSKY14 × CX1 hybridization of F1 (MSKY14 × CX1) seed
The seed of acquisition, similarly hereinafter).
Second step:It is female parent with F1 (MSKY14 × CX1), eggplant clothing kind CX1 is recurrent parent, and backcrossing obtains BC1 seed;
Using eggplant clothing kind CX1 as recurrent parent, and backcross progeny 5 generation of continuous backcross again, until obtaining BC6 seed, as cigar eggplant
The sterile line new varieties MSCX1 of clothing kind CX1.
Third step:It is restorer with eggplant clothing kind CX1, MSCX1 is sterile line, breeds the seed of MSCX1 production.
Third step:Cultivation of not pinching according to cigar tobacco produces routine techniques, by cigar eggplant clothing male sterile line MSCX1
Sowing, nursery, transplanting, fertilising, field management.Cigarette strain is not required to pinch after buddingging, and after proper mature (ripe), the harvesting of tobacco leaf Divisional priming leaf is dried in the air
System.
Effect example 1
By MSCX1, CX1 according to the same terms growing and cultivation, three groups are set, MSCX1 is processing group, the topping of CX1 kind
Cultivation be control 1, CX1 do not pinch cultivation be compare 2.Hack lever tied rope lodging-prevention is applied to control 2, other measures are identical.Compare
Quality of tobacco, the production labor cost of MSCX1 and control group that method provided by the invention obtains.Specific comparative situation is shown in Table 1
With table 2.
Table 1
Table 2
Processing | The amount of labour used/hm2 | Goods and materials cost (member/hm2) | Totle drilling cost (member/hm2) |
Processing | 0 | 0 | 0 |
Control 1 | 15 | 0 | 1500 |
Control 2 | 45 | 1500 | 6000 |
As can be seen from Table 1, relative to processing group (MSCX1), the nicotine of the tobacco leaf (control 1) of the plant for cultivation of pinching contains
Amount increases by 135%, and total nitrogen increases by 82.5%, and vane thickness increases by 61.7%, i.e., method provided by the invention can be effectively reduced
Tobacco leaf nicotine, nitrogen content, tobacco leaf thickness reduce.It is well known that nicotine, nitrogen content and tobacco leaf thickness are to directly affect tobacco leaf product
Matter determines that the important indicator of quality of tobacco, method provided by the invention can produce more good tobacco leaf compared with the existing technology.
As shown in Table 2, relatively conventional cultivation of not pinching, the MSCX1 that method provided by the invention obtains pinch and will not lodge due to being not necessarily to,
Totle drilling cost is zero, greatly reduces the topping or the expenditure to project resistant to lodging in cultivation, substantially reduces production cost.
Embodiment 2
As in the first embodiment, implementing this method using other kinds, the specific method is as follows:
The first step:Excellent cigar eggplant clothing tobacco main breed Havana92 is selected, male sterile line kind MS is selected
Yun yan85 is maternal, Havana92 as male parent using sterile line MS Yun yan85, and hybridization obtains F1 (MS Yun yan85 × Havana92) kind
Son.
Second step:It is female parent with F1 (MS Yun yan85 × Havana92), cigar eggplant clothing kind Havana92 is circulation parent
This, backcrossing obtains BC1 seed;5 generation of continuous backcross again, until obtaining BC6 seed, as cigar eggplant clothing kind Havana92's
Sterile line new varieties MS Havana92.
Third step:It is restorer with eggplant clothing kind Havana92, MS Havana92 is sterile line, breeds MS Havana92
The seed of production.
Third step:Cultivation of not pinching according to cigar tobacco produces routine techniques, by cigar eggplant clothing male sterile line MS
The sowing of Havana92 seed, nursery, transplanting, fertilising, field management.Cigarette strain is not required to pinch after buddingging, after proper mature (ripe), by tobacco leaf branch
Position priming leaf harvests the system of drying in the air.
Effect example 2
By MS Havana92, conventional H avana92 according to the same terms growing and cultivation, three groups, MS Havana92 are set
Cultivation do not pinch as processing group, conventional H avana92 kind pinches cultivation as control 1, and it is pair that conventional H avana92, which does not pinch and cultivates,
According to 2.2 application hack lever tied rope lodging-prevention of control, other measures are identical.The MS that method more provided by the invention obtains
The quality of tobacco of Havana92 and control group, topping and lodging-prevention production labor cost.Specific comparative situation is shown in Tables 1 and 2.
Table 3
Table 4
Processing | The amount of labour used/hm2 | Goods and materials cost (member/hm2) | Totle drilling cost (member/hm2) |
Processing | 0 | 0 | 0 |
Control 1 | 15 | 0 | 1500 |
Control 2 | 45 | 1500 | 6000 |
As can be seen from Table 3, relative to processing group (MS Havana92 does not pinch), the tobacco leaf of the plant for cultivation of pinching is (right
Increase according to nicotine, total nitrogen 1), vane thickness increases, i.e., tobacco leaf nicotine can be effectively reduced in method provided by the invention, nitrogen contains
Amount, tobacco leaf thickness reduce.As shown in Table 4, relatively conventional cultivation of not pinching, the technology that method provided by the invention obtains is due to being not necessarily to
It topping and will not lodge, this work totle drilling cost is zero, greatly reduces in cultivation the topping or to project resistant to lodging
Cost.
Contrast table 1 and table 3, table 2 and table 4 are it is found that nicotine of tobacco leaf of processing group etc. contains in embodiment 1 and embodiment 2
Amount is greatly lowered, and the reduction amplitude of amount of labour used etc. is larger.It is only exemplified by above-mentioned two embodiment as space is limited, but
From in the actual application of above-described embodiment and the method for offer of the invention it is found that method provided by the invention can be used for
A variety of male sterile lines cultivation cigars are not pinched eggplant clothing tobacco, however it is not limited to a certain kind.
The present invention is not limited to above-mentioned optional embodiment, anyone can show that other are each under the inspiration of the present invention
The product of kind form.Above-mentioned specific embodiment should not be understood the limitation of pairs of protection scope of the present invention, protection of the invention
Range should be subject to be defined in claims, and specification can be used for interpreting the claims.
Claims (9)
1. a kind of do not pinched the method for eggplant clothing tobacco using male sterile line cultivation cigar, which is characterized in that include following step
Suddenly:
It (1) is female parent with tobacco male sterile line kind, cigar eggplant clothing tobacco main breed is male parent, and hybridization obtains F1 generation kind
Son;
It (2) is female parent with F1 generation, cigar eggplant clothing tobacco main breed is recurrent parent, and backcrossing obtains BC1 seed, then continuously returns
It handed over for 5 generations, obtains BC6 seed, as cigar eggplant clothing male sterile line kind;
(3) using cigar eggplant clothing tobacco main breed used in step (1) as restorer, breeding cigar sterile line kind is raw
Production seed;
(4) cigarette strain that the cigar eggplant clothing sterile line variety seeds are sowed is not pinched after buddingging, and priming leaf harvests after proper mature (ripe)
The system of drying in the air is fermented after tobacco leaf modulation.
2. according to the method described in claim 1, it is characterized in that:The cigar eggplant clothing tobacco main breed include CX1 and
Havana92。
3. according to the method described in claim 2, it is characterized in that:The tobacco male sterile line kind is cultivation safflower tobacco
Sterile line.
4. according to the method described in claim 3, it is characterized in that:The tobacco male sterile line kind includes MSKY14 and MS
Yun yan85.
5. a kind of tobacco leaf as made from method as claimed in claim 4.
6. tobacco leaf according to claim 5, it is characterised in that:The tobacco leaf of the cigar eggplant clothing male sterile line kind
Nicotine content relative to the nicotine content of tobacco leaf that the topping cultivation of former cigar eggplant clothing tobacco main breed obtains reduce by 50% with
On.
7. tobacco leaf according to claim 5, it is characterised in that:The tobacco leaf of the cigar eggplant clothing male sterile line kind
Total nitrogen content relative to the total nitrogen content of tobacco leaf that the topping cultivation of former cigar eggplant clothing tobacco main breed obtains reduce by 50% with
On.
8. tobacco leaf according to claim 5, it is characterised in that:The tobacco leaf of the cigar eggplant clothing male sterile line kind
Vane thickness relative to the vane thickness of tobacco leaf that the topping cultivation of former cigar eggplant clothing tobacco main breed obtains reduce by 50% with
On.
9. application of the method in cigar tobacco cultivation described in a kind of claim 1-4 any one.
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Cited By (1)
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CN110692478A (en) * | 2019-11-13 | 2020-01-17 | 四川省烟草公司达州市公司 | Cigar tobacco cultivation method for one-plant production of eggplant-coated tobacco leaves and eggplant-cored tobacco leaves |
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CN110692478A (en) * | 2019-11-13 | 2020-01-17 | 四川省烟草公司达州市公司 | Cigar tobacco cultivation method for one-plant production of eggplant-coated tobacco leaves and eggplant-cored tobacco leaves |
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