CN108813152A - A kind of caragana microphylla base solid state fermentation feed and preparation method thereof - Google Patents
A kind of caragana microphylla base solid state fermentation feed and preparation method thereof Download PDFInfo
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- CN108813152A CN108813152A CN201810701314.2A CN201810701314A CN108813152A CN 108813152 A CN108813152 A CN 108813152A CN 201810701314 A CN201810701314 A CN 201810701314A CN 108813152 A CN108813152 A CN 108813152A
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- caragana microphylla
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- composite bacteria
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- 241000223025 Caragana microphylla Species 0.000 title claims abstract description 49
- 238000010563 solid-state fermentation Methods 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims description 9
- 241000894006 Bacteria Species 0.000 claims abstract description 46
- 239000002131 composite material Substances 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 18
- 244000025254 Cannabis sativa Species 0.000 claims abstract description 13
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 7
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 7
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 7
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 7
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims description 41
- 230000004151 fermentation Effects 0.000 claims description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 35
- 239000003795 chemical substances by application Substances 0.000 claims description 21
- 238000013329 compounding Methods 0.000 claims description 18
- 239000002994 raw material Substances 0.000 claims description 17
- 244000138286 Sorghum saccharatum Species 0.000 claims description 16
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 16
- 230000004913 activation Effects 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- 238000010298 pulverizing process Methods 0.000 claims description 7
- 230000003252 repetitive effect Effects 0.000 claims description 7
- 239000008187 granular material Substances 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims 1
- 241001061906 Caragana Species 0.000 abstract description 7
- 239000000463 material Substances 0.000 description 7
- 244000005700 microbiome Species 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002068 microbial inoculum Substances 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 244000062793 Sorghum vulgare Species 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 235000019713 millet Nutrition 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 206010007247 Carbuncle Diseases 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Mycology (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to field of feed, propose a kind of method of caragana microphylla base solid state fermentation feed, component including following quality proportioning, caragana microphylla 100-300, sugar grass 100-300, composite bacteria agent, the quality of the composite bacteria agent are the 0.8-1.2% of the caragana microphylla and the sugar grass quality sum, and the composite bacteria agent includes the component of following quality proportioning：Aspergillus oryzae 50-60, lactobacillus plantarum 40-50.A kind of method that the present invention also proposes caragana microphylla base solid state fermentation feed.Through the above technical solutions, solving the problems, such as to lack in the prior art, safety is good, caragana feed at low cost.
Description
Technical field
The invention belongs to caragana feed technical fields, are related to a kind of method of caragana microphylla base solid state fermentation feed.
Background technique
The auxiliary material of higher cost is needed to cooperate in existing caragana feed method mostly, such as bean powder, corn etc. causes to apply
Value is low, higher cost.The ensiling time is longer, generally requires 30~50 days.Partial fermentation microbial inoculum may have it is pathogenic, it is existing
Having listed bacillus subtilis in EU criteria cannot be used in feed microbial inoculum.
Need to add 5% or so corn flour, 0.2% urea, corn protein powder and wheat etc. in CN1539312A
It causes feed cost high, is unfavorable for commercialized running.
It includes bacillus subtilis that microbial inoculum is added in CN104605155A, and according to European Union's newest standards, bacillus subtilis
Bacterium may be not suitable for use in the degradation of cellulose in feed containing pathogenic.
Caragana feed fermentation time is longer in CN104543361A, after needing loess to seal 30 days.
Therefore, a kind of caragana feed method at low cost and easily operated production process or necessary is invented.
Summary of the invention
A kind of method that the present invention proposes caragana microphylla base solid state fermentation feed, solve lack in the prior art safety it is good,
The problem of caragana feed at low cost.
The technical proposal of the invention is realized in this way：
A kind of caragana microphylla base solid state fermentation feed, the component including following quality proportioning,
Caragana microphylla 100-300, sugar grass 100-300, composite bacteria agent,
The quality of the composite bacteria agent is the 0.8-1.2% of the caragana microphylla and the sugar grass quality sum,
The composite bacteria agent includes the component of following quality proportioning：Aspergillus oryzae 50-60, lactobacillus plantarum 40-50.
A kind of method that the present invention also proposes caragana microphylla base solid state fermentation feed, includes the following steps,
A) caragana microphylla and sweet sorghum stalk are uniformly mixed, obtain compounding raw material,
B) by composite bacteria agent and water according to 0.015~0.03:1 mass ratio is matched, wherein the temperature of water
It is 33-43 DEG C, places 1~2h of activation after mixing evenly, obtain bacterium solution,
C) water and step b) are added into the compounding raw material that step a) is obtained, obtains wet stock, and the wet stock
Water content be 65~70%,
D) wet stock is put into fermentation vat and forms fermentation layer, the fermentation layer is 10~15cm, then described
Ferment the layer upper surface sprinkling bacterium solution and water, compacting；Repetitive operation is until fermentation vat is filled.
Further, it remixes uniformly, obtains after the caragana microphylla and the sweet sorghum stalk being carried out pulverization process in step a)
To the compounding raw material.
Further, the caragana microphylla and the sweet sorghum stalk are subjected to pulverization process, obtain 4~10cm of length, fineness degree
Filiform or granular material less than or equal to 2mm.
Beneficial effects of the present invention are：
1, the non-grain fodder in the present invention mainly carries out solid state fermentation with caragana microphylla and sweet sorghum stalk compounding, auxiliary without other
Material addition.The reason is that having soluble sugar abundant in sweet sorghum stalk, required carbon source can be provided for microorganism.Due to sweet tea height
There is soluble sugar abundant in fine strain of millet stalk, easily utilized by microorganism, shorten starting of microorganism during ensiling
Time, while there are the fungus strains of decomposition of cellulose in composite bacteria agent, still can be micro- life when soluble sugar content reduces
Carbon source needed for object provides, it is possible to greatly shorten the ensiling period.
2, caragana microphylla and sweet sorghum stalk are handled by beneficial microorganism, and protein content in caragana microphylla can be made full use of higher high with sweet tea
The soluble sugar being rich in fine strain of millet stalk reduces anti-battalion to improve plant-eating animal to coarse-fibred digestive utilization ratio and palatability
Support the factor.Biological compound feed of the invention is full of nutrition, and protein content is high, absorption easy to digest, and containing abundant vitamin and
Minerals, amino acid etc..
The advantages that caragana microphylla, which has, checks winds and fixes drifting sand, and resists poor carbuncle, resistance to dry early, is a kind of recyclable regeneration Shrub populations,
Caragana microphylla is fabricated to Caragana Microphylla biological mixed feed, plantation fodder grass can be not only reduced and occupy the area in farmland, and can solve
Because stump caragana microphylla can not efficiently use, and problem of environmental pollution caused by burning.
Caragana Microphylla biological mixed feed of the invention is at low cost, and the solid state fermentation production process of use is easily operated, technique at
This is lower.
3, it will especially be handled after caragana microphylla and sugar grass pulverization process as 4~10cm of length, fineness degree is less than or equal to 2mm's
Filamentous or granular material, can contribute to ferment, and increase the uniformity coefficient of feed after fermentation, to improve feeding quality.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1：
A kind of caragana microphylla base solid state fermentation feed, the component including following quality proportioning,
Caragana microphylla 100Kg, sugar grass 300Kg, composite bacteria agent 320g,
The composite bacteria agent includes the component of following quality proportioning：Aspergillus oryzae 50, lactobacillus plantarum 40.
Preparation method is as follows,
A) caragana microphylla and sweet sorghum stalk are uniformly mixed, obtain compounding raw material,
B) by composite bacteria agent and water according to 0.015:1 mass ratio is matched, wherein the temperature of water is 33-
43 DEG C, activation 1h is placed after mixing evenly, obtains bacterium solution,
C) bacterium solution that water and step b) are obtained is added into the compounding raw material that step a) is obtained, what is made is wet
The water content of material is 65%,
D) wet stock is put into fermentation vat and forms fermentation layer, the fermentation layer is 10~15cm, then described
Ferment the layer upper surface sprinkling bacterium solution and water, compacting；Repetitive operation is filled up to fermentation vat,
E) it is sealed by fermentation.
Embodiment 2：
A kind of caragana microphylla base solid state fermentation feed, the component including following quality proportioning,
Caragana microphylla 300Kg, sugar grass 100Kg, composite bacteria agent 480g,
The composite bacteria agent includes the component of following quality proportioning：Aspergillus oryzae 50, lactobacillus plantarum 40.
Preparation method is as follows,
A) caragana microphylla and sweet sorghum stalk are uniformly mixed, obtain compounding raw material,
B) by composite bacteria agent and water according to 0.03:1 mass ratio is matched, wherein the temperature of water is 33-43
DEG C, activation 2h is placed after mixing evenly, obtains bacterium solution,
C) bacterium solution that water and step b) are obtained is added into the compounding raw material that step a) is obtained, what is made is wet
The water content of material is 70%,
D) wet stock is put into fermentation vat and forms fermentation layer, the fermentation layer is 10~15cm, then described
Ferment the layer upper surface sprinkling bacterium solution and water, compacting；Repetitive operation is filled up to fermentation vat,
E) it is sealed by fermentation.
Embodiment 3：
A kind of caragana microphylla base solid state fermentation feed, the component including following quality proportioning,
Caragana microphylla 100Kg, sugar grass 100Kg, composite bacteria agent 200g,
The composite bacteria agent includes the component of following quality proportioning：Aspergillus oryzae 55, lactobacillus plantarum 45.
Preparation method is as follows,
A) caragana microphylla and sweet sorghum stalk are uniformly mixed, obtain compounding raw material,
B) by composite bacteria agent and water according to 0.02:1 mass ratio is matched, wherein the temperature of water is 33-43
DEG C, activation 1h is placed after mixing evenly, obtains bacterium solution,
C) bacterium solution that water and step b) are obtained is added into the compounding raw material that step a) is obtained, what is made is wet
The water content of material is 67%,
D) wet stock is put into fermentation vat and forms fermentation layer, the fermentation layer is 10~15cm, then described
Ferment the layer upper surface sprinkling bacterium solution and water, compacting；Repetitive operation is filled up to fermentation vat,
E) it is sealed by fermentation.
Embodiment 4：
A kind of caragana microphylla base solid state fermentation feed, the component including following quality proportioning,
Caragana microphylla 100Kg, sugar grass 100Kg, composite bacteria agent 200g,
The composite bacteria agent includes the component of following quality proportioning：Aspergillus oryzae 55, lactobacillus plantarum 45.
Preparation method is as follows,
A) caragana microphylla and the sweet sorghum stalk are subjected to pulverization process, obtain 4~10cm of length, fineness degree is less than etc.
It is uniformly mixed in the filiform or granular material of 2mm, then by smashed caragana microphylla and sweet sorghum stalk, obtains compounding raw material,
B) by composite bacteria agent and water according to 0.02:1 mass ratio is matched, wherein the temperature of water is 33-43
DEG C, activation 1h is placed after mixing evenly, obtains bacterium solution,
C) bacterium solution that water and step b) are obtained is added into the compounding raw material that step a) is obtained, what is made is wet
The water content of material is 67%,
D) wet stock is put into fermentation vat and forms fermentation layer, the fermentation layer is 10~15cm, then described
Ferment the layer upper surface sprinkling bacterium solution and water, compacting；Repetitive operation is filled up to fermentation vat,
E) it is sealed by fermentation.
Comparative example 1：
Caragana microphylla 100Kg, sugar grass 100Kg, commercially available Junan em bacterium ensiling stalks leavening 80g,
Preparation method is as follows,
A) caragana microphylla and the sweet sorghum stalk are subjected to pulverization process, obtain 4~10cm of length, fineness degree is less than etc.
It is uniformly mixed in the filiform or granular material of 2mm, then by smashed caragana microphylla and sweet sorghum stalk, obtains compounding raw material,
B) by Junan em bacterium ensiling stalks leavening and water according to 0.02:1 mass ratio is matched, wherein water
Temperature is 33-43 DEG C, places activation 1h after mixing evenly, obtains bacterium solution,
C) bacterium solution that water and step b) are obtained is added into the compounding raw material that step a) is obtained, what is made is wet
The water content of material is 67%,
D) wet stock is put into fermentation vat and forms fermentation layer, the fermentation layer is 10~15cm, then described
Ferment the layer upper surface sprinkling bacterium solution and water, compacting；Repetitive operation is filled up to fermentation vat,
E) it is sealed by fermentation.
Feed made from above-described embodiment is detected, it is as a result as follows：
It follows that the caragana feed in the present invention is low in cost, production method is simple, and the compound feed egg produced
Bai Hanliang high, crude fiber content are low.And the fermentation period in above-described embodiment is in 10-15, more similar compound feed fermentation time
It is short.In comparative example 1 feed preparation feed not only fermentation time at 30 days or so, but also prepare crude protein content of feed
Low, the feed value prepared is lower.
The above is merely preferred embodiments of the present invention, be not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (4)
1. a kind of caragana microphylla base solid state fermentation feed, it is characterised in that：Component including following quality proportioning,
Caragana microphylla 100-300, sugar grass 100-300, composite bacteria agent,
The quality of the composite bacteria agent is the 0.8-1.2% of the caragana microphylla and the sugar grass quality sum,
The composite bacteria agent includes the component of following quality proportioning：Aspergillus oryzae 50-60, lactobacillus plantarum 40-50.
2. a kind of preparation method of caragana microphylla base solid state fermentation feed, it is characterised in that：Include the following steps,
A) caragana microphylla and sweet sorghum stalk are uniformly mixed, obtain compounding raw material,
B) by composite bacteria agent and water according to 0.015~0.03:1 mass ratio is matched, wherein the temperature of water is
33-43 DEG C, 1~2h of activation is placed after mixing evenly, obtains bacterium solution,
C) the obtained bacterium solution of water and step b) is added in the compounding raw material obtained to step a), obtains wet stock,
And the water content of the wet stock is 65~70%,
D) wet stock is put into fermentation vat and forms fermentation layer, the fermentation layer is 10~15cm, then in the fermentation
Spray the bacterium solution and water, compacting in layer upper surface；Repetitive operation is filled up to fermentation vat,
E) it is sealed by fermentation,
Step a) and the part step b) forward/backward operation sequence.
3. a kind of method of caragana microphylla base solid state fermentation feed according to claim 2, it is characterised in that：By institute in step a)
It is remixed uniformly after stating caragana microphylla and sweet sorghum stalk progress pulverization process, obtains the compounding raw material.
4. a kind of method of caragana microphylla base solid state fermentation feed according to claim 3, it is characterised in that：By the caragana microphylla and
The sweet sorghum stalk carries out pulverization process, obtains 4~10cm of length, and fineness degree is less than or equal to the filiform or granular material of 2mm,
Then it is uniformly mixed, obtains the compounding raw material.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810701314.2A CN108813152A (en) | 2018-06-29 | 2018-06-29 | A kind of caragana microphylla base solid state fermentation feed and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810701314.2A CN108813152A (en) | 2018-06-29 | 2018-06-29 | A kind of caragana microphylla base solid state fermentation feed and preparation method thereof |
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Application publication date: 20181116 |