CN108785552A - A kind of preparation method of giantreed rhizome composition of alkaloids - Google Patents
A kind of preparation method of giantreed rhizome composition of alkaloids Download PDFInfo
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- CN108785552A CN108785552A CN201810773733.7A CN201810773733A CN108785552A CN 108785552 A CN108785552 A CN 108785552A CN 201810773733 A CN201810773733 A CN 201810773733A CN 108785552 A CN108785552 A CN 108785552A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a kind of preparation methods of giantreed rhizome composition of alkaloids, and steps are as follows：Giantreed rhizome medicinal material coarse powder is soaked into 4-6h, decocts 2-3h, filtered through gauze, the dregs of a decoction decoct once again, merge decocting liquid and concentrate, being adjusted to containing alcohol 70-80% with ethyl alcohol carries out alcohol precipitation, stand 23-25h, it filters, filtrate concentrates to recycle ethyl alcohol, and it is 10.5-11.5 that alcohol medicinal extract, which is alkalized with concentrated ammonia liquor to pH, with chloroform extraction 4-6 times, merge chloroform layer, detached through alkaline silica gel column chromatography after reduced pressure, uses volume ratio 45 successively:1,35:1,25:1,18:1,1:1 methylene chloride-methanol elution, collects 18:1 elution fraction, concentrates to obtain the final product.This method is easy, easy to operate, is successfully prepared composition of alkaloids in giantreed rhizome, has the function of being depressured spasmolysis, can be prepared applied to drug as the active component of giantreed rhizome.
Description
Technical field
The present invention relates to a kind of preparation methods of giantreed rhizome composition of alkaloids.
Background technology
Giantreed rhizome Arundo Donax Rhizoma also known as Lu Ditou, stair bar are grass family giantreed platymiscium giantreedArundo donax L. fresh or dry rhizome, be used as medicine first recorded in《The south of the Five Ridges is gathered medicinal herbs record》.Giantreed is that the former of giantreed rhizome is planted
Object also known as reed giantreed（《Collected statements on the herbal foundation》）, green bamboo（《Classify herbal》）, it is a kind of plant that distribution on global is wider, resource
It is abundant, it is distributed widely in Asia, south of europe, Africa the north and Middle East.China is distributed mainly on southwest, south China and river
The ground such as Soviet Union, Zhejiang, Hunan.Giantreed is chiefly used in paper industry in China.Giantreed is used as cicarisantia, hemostasis on the ground such as Europe and India
Medicine, lactifuge, antidiarrheal agent, diuretics, or for treat have a toothache, bronchitis etc., medical value is notable.Giantreed rhizome slight bitter, taste
It is sweet, it is cold in nature, have the function of clearing heat-fire, preventing or arresting vomiting promote the production of body fluid, inducing diuresis for treating strangurtia, cure mainly pyreticosis go mad, consumptive disease hectic fever due to yin, difficult urination,
The diseases such as gonorrhoea, acute toothache.Clinically it is mainly used for hyperpyrexia caused by treating children's upper respiratory tract infection, it is curative for effect, be
The primary raw material medicinal material of Chinese medicine preparation reed Huang electuary and cold fever eliminating injection.Giantreed rhizome main chemical compositions are given birth to for a variety of indoles
Alkaloids, there is a decompression spasmolysis, can spasm caused by antagonism histamine, serotonin, acetylcholine.Therefore, giantreed rhizome have compared with
Good medicinal Development volue.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of giantreed rhizome composition of alkaloids.
The present invention is realized by following technical solution：
A kind of preparation method of giantreed rhizome composition of alkaloids, includes the following steps：Giantreed rhizome medicinal material coarse powder is soaked into 4-
6h decocts 2-3h, filtered through gauze, and the dregs of a decoction decoct once again, merges decocting liquid and concentrates, being adjusted to containing alcohol 70-80% with ethyl alcohol carries out
Alcohol precipitation stands 23-25h, filters, and filtrate concentrates to recycle ethyl alcohol, and it is 10.5-11.5 that alcohol medicinal extract, which is alkalized with concentrated ammonia liquor to pH, uses
Chloroform extraction 4-6 times merges chloroform layer, is detached through alkaline silica gel column chromatography after reduced pressure, uses body successively
Product ratio 45:1,35:1,25:1,18:1,1:1 methylene chloride-methanol elution, collects 18:1 elution fraction, concentrates to obtain the final product.
Preferably, in the preparation method, giantreed rhizome medicinal material coarse powder is soaked into 5h.
Preferably, in the preparation method, 2.5h is decocted.
Preferably, it in the preparation method, is adjusted to containing alcohol 75% with ethyl alcohol and carries out alcohol precipitation.
Preferably, in the preparation method, it is static for 24 hours.
Preferably, in the preparation method, it is 11 that alcohol medicinal extract, which is alkalized with concentrated ammonia liquor to pH,.
Preferably, in the preparation method, with chloroform extraction 5 times.
Preferably, in the preparation method, 1kg giantreed rhizomes carry out immersion decoction using 9-11L water.
The technology of the present invention effect：
This method is easy, easy to operate, is successfully prepared composition of alkaloids in giantreed rhizome, has the function of decompression spasmolysis, can be with
Active component as giantreed rhizome is prepared applied to drug.
Specific implementation mode
The essentiality content of the present invention is specifically introduced with reference to embodiment.
Embodiment 1
A kind of preparation method of giantreed rhizome composition of alkaloids, includes the following steps：Giantreed rhizome medicinal material coarse powder is soaked into 5h,
2.5h, filtered through gauze are decocted, the dregs of a decoction decoct once again, merge decocting liquid and concentrate, and being adjusted to containing alcohol 75% with ethyl alcohol carries out alcohol precipitation,
It standing for 24 hours, filters, to recycle ethyl alcohol, it is 11 that alcohol medicinal extract, which is alkalized with concentrated ammonia liquor to pH, for filtrate concentration, with chloroform extraction 5 times,
Merge chloroform layer, detached through alkaline silica gel column chromatography after reduced pressure, uses volume ratio 45 successively:1,35:1,25:1,
18:1,1:1 methylene chloride-methanol elution, collects 18:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg giantreed rhizomes carry out immersion decoction using 10L water.
Embodiment 2
A kind of preparation method of giantreed rhizome composition of alkaloids, includes the following steps：Giantreed rhizome medicinal material coarse powder is soaked into 4h,
2h, filtered through gauze are decocted, the dregs of a decoction decoct once again, merge decocting liquid and concentrate, being adjusted to containing alcohol 70% with ethyl alcohol carries out alcohol precipitation, quiet
23h to be set, is filtered, to recycle ethyl alcohol, it is 10.5 that alcohol medicinal extract, which is alkalized with concentrated ammonia liquor to pH, for filtrate concentration, with chloroform extraction 4 times,
Merge chloroform layer, detached through alkaline silica gel column chromatography after reduced pressure, uses volume ratio 45 successively:1,35:1,25:1,
18:1,1:1 methylene chloride-methanol elution, collects 18:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg giantreed rhizomes carry out immersion decoction using 9L water.
Embodiment 3
A kind of preparation method of giantreed rhizome composition of alkaloids, includes the following steps：Giantreed rhizome medicinal material coarse powder is soaked into 6h,
3h, filtered through gauze are decocted, the dregs of a decoction decoct once again, merge decocting liquid and concentrate, and being adjusted to containing alcohol 70-80% with ethyl alcohol carries out alcohol precipitation,
25h is stood, is filtered, filtrate concentration is to recycle ethyl alcohol, and it is 11.5 that alcohol medicinal extract, which is alkalized with concentrated ammonia liquor to pH, with chloroform extraction 6
It is secondary, merge chloroform layer, detached through alkaline silica gel column chromatography after reduced pressure, uses volume ratio 45 successively:1,35:1,
25:1,18:1,1:1 methylene chloride-methanol elution, collects 18:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg giantreed rhizomes carry out immersion decoction using 11L water.
This method is easy, easy to operate, is successfully prepared composition of alkaloids in giantreed rhizome, has the function of being depressured spasmolysis,
It can be prepared applied to drug as the active component of giantreed rhizome.
Claims (8)
1. a kind of preparation method of giantreed rhizome composition of alkaloids, it is characterised in that include the following steps：Giantreed root herb is thick
Powder soaks 4-6h, decocts 2-3h, filtered through gauze, and the dregs of a decoction decoct once again, merges decocting liquid and concentrates, is adjusted to contain with ethyl alcohol
Alcohol 70-80% carries out alcohol precipitation, stands 23-25h, filters, and filtrate concentrates to recycle ethyl alcohol, and alcohol medicinal extract is alkalized with concentrated ammonia liquor is to pH
10.5-11.5 merges chloroform layer with chloroform extraction 4-6 times, through alkaline silica gel column chromatography point after reduced pressure
From successively with volume ratio 45:1,35:1,25:1,18:1,1:1 methylene chloride-methanol elution, collects 18:1 elution fraction is dense
It contracts to obtain the final product.
2. preparation method according to claim 1, it is characterised in that：Giantreed rhizome medicinal material coarse powder is soaked into 5h.
3. preparation method according to claim 1, it is characterised in that：Decoct 2.5h.
4. preparation method according to claim 1, it is characterised in that：It is adjusted to containing alcohol 75% with ethyl alcohol and carries out alcohol precipitation.
5. preparation method according to claim 1, it is characterised in that：It is static for 24 hours.
6. preparation method according to claim 1, it is characterised in that：It is 11 that alcohol medicinal extract, which is alkalized with concentrated ammonia liquor to pH,.
7. preparation method according to claim 1, it is characterised in that：With chloroform extraction 5 times.
8. preparation method according to claim 1, it is characterised in that：1kg giantreed rhizomes using 9-11L water impregnate and be decocted
It boils.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810773733.7A CN108785552A (en) | 2018-07-15 | 2018-07-15 | A kind of preparation method of giantreed rhizome composition of alkaloids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810773733.7A CN108785552A (en) | 2018-07-15 | 2018-07-15 | A kind of preparation method of giantreed rhizome composition of alkaloids |
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| Publication Number | Publication Date |
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| CN108785552A true CN108785552A (en) | 2018-11-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810773733.7A Withdrawn CN108785552A (en) | 2018-07-15 | 2018-07-15 | A kind of preparation method of giantreed rhizome composition of alkaloids |
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| CN (1) | CN108785552A (en) |
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2018
- 2018-07-15 CN CN201810773733.7A patent/CN108785552A/en not_active Withdrawn
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Application publication date: 20181113 |