CN108782238A - A kind of method of morning pears stem with bud Plantlet in vitro - Google Patents
A kind of method of morning pears stem with bud Plantlet in vitro Download PDFInfo
- Publication number
- CN108782238A CN108782238A CN201810445955.6A CN201810445955A CN108782238A CN 108782238 A CN108782238 A CN 108782238A CN 201810445955 A CN201810445955 A CN 201810445955A CN 108782238 A CN108782238 A CN 108782238A
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- bud
- culture
- culture medium
- vitro
- stem
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The method of early pears stem with bud Plantlet in vitro：Plantlet in vitro carried out to early pears stem with bud successively with four kinds of culture mediums containing carbon nanotube or graphene quantum dot, subculture again after 360d is viable to form complete test tube seedling.Compared with the prior art, the early pears stem with bud Plantlet in vitro time is long, survival rate is high, Plantlet in vitro effect is good by the present invention.
Description
Technical field：
The present invention relates to a kind of method of plant stem with bud Plantlet in vitro, be exactly a kind of early pears stem with bud from
The method that body preserves.
Background technology：
The excellent local jargonel germ plasm resource of the Jiangxi Zao Lishi, Shangrao Shangrao County reception room or parlour and the towns Tian Dundeng.Shangrao morning pears
There are two main breed is general, i.e. " serissa serissoide " and " Calusena lansium disappears ", wherein " serissa serissoide " quality is even better, be Shangrao morning pears most
Improved seeds, it is ripe early, and epidermis it is thin it is thin, meat is tender and crisp, core lacks that juice is more, taste is fragrant and sweet, the Qing Dynasty is once recommended as tribute.Currently,
The form that Shangrao morning pears germ plasm resource mostly uses greatly field gene bank preserves, time-consuming, laborious, costly, and is vulnerable to natural calamity and people
For influences such as errors.Plantlet in vitro is to utilize the means of Plant Tissue Breeding are interior in a limited space to preserve vegetable material, is had
Land used is few, it is artificial less, the advantages that consumption is few, be now widely used in the Germ-plasma resources protection of various Local Excellent plants.Plantlet in vitro
The shortcomings that be preserve during be easy that culture medium and moisture is made to gradually decrease, cause Plantlet in vitro vegetable material be easy death,
The purpose of long-term Plantlet in vitro in can not realizing for a long time.For these problems, the present invention develops a kind of early pears stem with bud
The method of Plantlet in vitro can provide technical foundation for the medium-term and long-term preservation of Shangrao morning pears germ plasm resource.
Invention content：
The object of the present invention is to provide the early pears bands that a kind of Plantlet in vitro time is long, survival rate is high, Plantlet in vitro effect is good
The method of leaf stem section Plantlet in vitro.Realizing the technical solution of the object of the invention is, a kind of side of morning pears stem with bud Plantlet in vitro
Method, it is characterised in that have following steps：(1) it in superclean bench, cuts early pears stem with bud and is inoculated in culture medium
Material is put into culturing room after inoculation and carries out conventional strip by one (MS+0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar)
Part culture, culturing room's condition of culture are：25 ± 2 DEG C, light application time 14h/d, light intensity 1000-1500lx of temperature；(2) in culture medium
After cultivating 7-10d on one, early pears stem with bud is transferred to the (MS+0.3-0.5g/L graphene quantum dot+30g/L sugarcanes of culture medium two
Sugar+0g/L agar), material is put into culturing room after inoculation and carries out normal condition culture, culturing room's condition of culture is：Temperature 25 ±
2 DEG C, light application time 14h/d, light intensity 1000-1500lx；(3) after cultivating 7-10d on culture medium two, early pears stem with bud is turned
Enter culture medium three (MS+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dot+30g/L sucrose+0g/L agar),
Material is put into culturing room after inoculation and carries out normal condition culture, culturing room's condition of culture is：25 ± 2 DEG C of temperature, light application time
14h/d, light intensity 1000-1500lx；(4) after cultivating 7-10d on culture medium three, early pears stem with bud is transferred to culture medium four
(MS+1-2mg/L Thidiazuron+0.2-0.5mg/L IBA+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dots+
30g/L sucrose+7.5g/L agar), material is put into culturing room after inoculation and carries out normal condition culture, culturing room's condition of culture
For：25 ± 2 DEG C, light application time 14h/d, light intensity 1000-1500lx of temperature；(5) after cultivating 360d on culture medium four, by early pears
Stem with bud takes out, renewed vaccination to subculture medium (MS+1-2mg/L Thidiazuron+0.2-0.5mg/L IBA+30g/L sucrose+
7.5g/L agar) on carry out normal condition culture, culturing room's condition of culture is：25 ± 2 DEG C, light application time 16h/d of temperature, light intensity
1500-2500lx, it is viable to form complete test tube seedling.
Specific implementation mode：
In conjunction with following embodiments, the invention will be further described：
(1) it cultivates for the first time
In superclean bench, cuts early pears stem with bud and be inoculated in culture medium one (MS+0.3-0.5g/L carbon is received
Mitron+30g/L sucrose+0g/L agar), material is put into culturing room after inoculation and carries out normal condition culture, item is cultivated by culturing room
Part is：25 ± 2 DEG C, light application time 16h/d, light intensity 1500-2500lx of temperature；
(2) it cultivates for second
After cultivating 7-10d on culture medium one, early pears stem with bud is transferred to (the MS+0.3-0.5g/L graphite of culture medium two
Alkene quantum dot+30g/L sucrose+0g/L agar), material is put into culturing room after inoculation and carries out normal condition culture, culturing room's training
Foster condition is：25 ± 2 DEG C, light application time 16h/d, light intensity 1500-2500lx of temperature；
(3) third time is cultivated
After cultivating 7-10d on culture medium two, early pears stem with bud is transferred to culture medium three, and (MS+0.3-0.5g/L carbon is received
Mitron+0.3-0.5g/L graphene quantum dot+30g/L sucrose+0g/L agar), material is put into culturing room after inoculation and is carried out often
CMC model is advised, culturing room's condition of culture is：25 ± 2 DEG C, light application time 16h/d, light intensity 1500-2500lx of temperature；
(4) the 4th cultures
On culture medium three cultivate 7-10d after, by early pears stem with bud be transferred to culture medium four (MS+1-2mg/L Thidiazurons+
0.2-0.5mg/L IBA+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dot+30g/L sucrose+7.5g/L fine jades
Fat), material is put into culturing room after inoculation and carries out normal condition culture, culturing room's condition of culture is：25 ± 2 DEG C of temperature, illumination
Time 16h/d, light intensity 1500-2500lx；
After cultivating 360d on culture medium four, early pears stem with bud is taken out, renewed vaccination to subculture medium (MS+1-
2mg/L Thidiazuron+0.2-0.5mg/L IBA+30g/L sucrose+7.5g/L agar) on carry out normal condition culture, culturing room training
Foster condition is：25 ± 2 DEG C, light application time 16h/d of temperature, light intensity 1500-2500lx, the viable complete test tube seedling of formation.
Claims (1)
1. the method for early pears stem with bud Plantlet in vitro, it is characterised in that there is following steps：(1) it in superclean bench, cuts
Early pears stem with bud is simultaneously inoculated in culture medium one：MS+0.3-0.5g/L carbon nanotube+30g/L sucrose+0g/L agar, connects
Material is put into culturing room after kind and carries out normal condition culture；(2) after cultivating 7-10d on culture medium one, by early pears stem segment with bud
Section is transferred to culture medium two：Material, is put by MS+0.3-0.5g/L graphene quantum dot+30g/L sucrose+0g/L agar after inoculation
Culturing room carries out normal condition culture；(3) after cultivating 7-10d on culture medium two, early pears stem with bud is transferred to culture medium three：
MS+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dot+30g/L sucrose+0g/L agar, by material after inoculation
It is put into culturing room and carries out normal condition culture；(4) after cultivating 7-10d on culture medium three, early pears stem with bud is transferred to culture
Base four：MS+1-2mg/L Thidiazuron+0.2-0.5mg/L IBA+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantums
+ 30g/L sucrose+7.5g/L agar is selected, material, which is put into culturing room, after inoculation carries out normal condition culture；(5) in culture medium four
After upper culture 360d, early pears stem with bud is taken out, again subculture, it is viable to form complete test tube seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810445955.6A CN108782238A (en) | 2018-05-03 | 2018-05-03 | A kind of method of morning pears stem with bud Plantlet in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810445955.6A CN108782238A (en) | 2018-05-03 | 2018-05-03 | A kind of method of morning pears stem with bud Plantlet in vitro |
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| Publication Number | Publication Date |
|---|---|
| CN108782238A true CN108782238A (en) | 2018-11-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810445955.6A Pending CN108782238A (en) | 2018-05-03 | 2018-05-03 | A kind of method of morning pears stem with bud Plantlet in vitro |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255707A (en) * | 2014-09-15 | 2015-01-07 | 上海交通大学 | Method for improving preservation effect of cymbidium type protocorm |
| CN104255708A (en) * | 2014-09-15 | 2015-01-07 | 上海交通大学 | Method for optimizing vitrification ultra-low temperature preservation effect of cymbidium type protocorm |
-
2018
- 2018-05-03 CN CN201810445955.6A patent/CN108782238A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255707A (en) * | 2014-09-15 | 2015-01-07 | 上海交通大学 | Method for improving preservation effect of cymbidium type protocorm |
| CN104255708A (en) * | 2014-09-15 | 2015-01-07 | 上海交通大学 | Method for optimizing vitrification ultra-low temperature preservation effect of cymbidium type protocorm |
Non-Patent Citations (5)
| Title |
|---|
| 杨芳: "秋子梨组织培养与离体保存的研究", 《万方数据知识服务平台》 * |
| 洪森荣等: "离体保存技术在植物种质资源保存中的应用 ", 《上饶师范学院学报》 * |
| 赵振杰等: "碳纳米材料对植物生长发育的调节作用", 《作物杂志》 * |
| 赵胜利: "桃梨离体再生体系建立及试管苗种质保存研究", 《万方数据知识服务平台》 * |
| 邵建柱: "苹果等果树种质资源的离体保存研究", 《万方数据知识服务平台》 * |
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Application publication date: 20181113 |