CN108575755A - A kind of method of morning pears androgenesis - Google Patents
A kind of method of morning pears androgenesis Download PDFInfo
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- CN108575755A CN108575755A CN201810445952.2A CN201810445952A CN108575755A CN 108575755 A CN108575755 A CN 108575755A CN 201810445952 A CN201810445952 A CN 201810445952A CN 108575755 A CN108575755 A CN 108575755A
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- culture medium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
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- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The method of early pears androgenesis：Androgenesis carried out to early Pear leaves successively with four kinds of culture mediums containing carbon nanotube, after 120 240d, more test tube seedling can be formed.Compared with the prior art, early pears androgenesis is fast by the present invention, and the test tube seedling quantity formed is more.
Description
Technical field：
The present invention relates to a kind of methods of plant callus induction and differentiation, are exactly a kind of early pears callus
The method of induction and differentiation.
Background technology：
Shangrao morning pears are rose family rose, and principal item has serissa serissoide and Calusena lansium to disappear, in Jiangxi Shangrao County master
It is distributed in the small towns such as the reception room or parlour town towns He Tiandun.Shangrao morning pears, it is ripe early, and epidermis is thin, pulp is tender, sweet and dilitious, has life
It is Tianjin moistening lung, clearing heat and eliminating phlegm, antipyretic the effect of relieving summer heat, suitable for people of all ages.Shangrao morning pears are full of nutrition, according to surveying and determination, 100g apples containing 8g
Acid, 8g glucose, 10g carbohydrate, 1g protein, 0.1g fat, 5mg calcium, 6mg phosphorus.Shangrao morning pears can not only be eaten raw,
It is alternatively arranged as the excellent raw material of Chinese patent drug " snow pear paste ".In recent years, the planting industry of Shangrao morning pears is rapidly developed, on
The Agricultural Integration Leading Enterprises of rich morning pears are continuously increased, and Shangrao production bases Zao Li are up to 2.2 ten thousand mu, and yield is up to more than 30,000 tons.
But due to the excellent local varieties of spy of the Jiangxi Zao Lishi, Shangrao jargonel, distribution is relatively narrow, and genuineness wild resource is less.Using
Plant tissue culture technique can quickly breed Shangrao morning pears seedling, realize its factorial praluction.Androgenesis
It is the fast numerous effective way of plant seedling, but the induction of pears callus and differentiation efficiency be not high at present, browning is more tight
Weight, power of regeneration is not strong, and the large-scale cultivation of pears seedling can not be really realized by the induction of callus and differentiation.For
These problems, the present invention develop a kind of method of early pears androgenesis, can be the work of Shangrao morning pears test tube seedling
The production of industry metaplasia is provided for a long term technical foundation.
Invention content：
The object of the present invention is to provide a kind of browning reduction, the early pears callus inductions that survival rate is high, detoxification efficiency is good
With the method for differentiation.Realizing the technical solution of the object of the invention is, a kind of method of morning pears androgenesis, special
Sign is there are following steps：(1) in superclean bench, early pears test tube seedling leaf is cut, and its back side is inoculated in training upward
Support one (MS+2-3mg/L Thidiazuron+0.3-0.5mg/L IBA+0.3-0.5g/L carbon nanotube+30g/L sucrose+7.5g/L fine jades of base
Fat), material is put into culturing room after inoculation and carries out normal condition dark culturing, the temperature of culturing room is 25 ± 2 DEG C；(2) it is training
It supports after cultivating 30-60d on base one, early pears callus is transferred to the (MS+1-2mg/L Thidiazurons+0.2-0.5mg/L of culture medium two
IBA+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dot+30g/L sucrose+7.5g/L agar), it will after inoculation
Material is put into culturing room and carries out normal condition culture, and culturing room's condition of culture is：25 ± 2 DEG C, light application time 16h/d of temperature, light
Strong 1500-2500lx；(3) after cultivating 30-60d on culture medium two, jargonel simple bud is transferred to three (MS+1-2mg/L of culture medium
Thidiazuron+0.2-0.5mg/L IBA+0.3-0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar), by material after inoculation
It is put into culturing room and carries out normal condition culture, culturing room's condition of culture is：25 ± 2 DEG C, light application time 16h/d of temperature, light intensity
1500-2500lx；(4) after cultivating 30-60d on culture medium three, jargonel adventitious bud is transferred to four (1/2MS+0.5- of culture medium
1mg/L IBA+0.1-0.3mg/L PP333+ 0.3-0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar), it will after inoculation
Material is put into culturing room and carries out normal condition culture, and culturing room's condition of culture is：25 ± 2 DEG C, light application time 16h/d of temperature, light
Strong 1500-2500lx；(5) after cultivating 30-60d on culture medium four, jargonel adventitious bud rooting forms complete healthy and strong test tube
Seedling.
Specific implementation mode：
In conjunction with following embodiments, the invention will be further described：
(1) callus induction
In superclean bench, early pears test tube seedling leaf is cut, and its back side is inoculated in one (MS+2- of culture medium upward
3mg/L Thidiazuron+0.3-0.5mg/L IBA+0.3-0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar), it will after inoculation
Material is put into culturing room and carries out normal condition dark culturing, and the temperature of culturing room is 25 ± 2 DEG C；
(2) callus differentiation budding
On culture medium one cultivate 30-60d after, by early pears callus be transferred to culture medium two (MS+1-2mg/L Thidiazurons+
0.2-0.5mg/L IBA+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dot+30g/L sucrose+7.5g/L fine jades
Fat), material is put into culturing room after inoculation and carries out normal condition culture, culturing room's condition of culture is：25 ± 2 DEG C of temperature, illumination
Time 16h/d, light intensity 1500-2500lx；
(3) regeneration bud is proliferated
On culture medium two cultivate 30-60d after, by jargonel simple bud be transferred to culture medium three (MS+1-2mg/L Thidiazurons+
0.2-0.5mg/L IBA+0.3-0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar), material is put into culture after inoculation
Room carries out normal condition culture, and culturing room's condition of culture is：25 ± 2 DEG C, light application time 16h/d, light intensity 1500- of temperature
2500lx；
(4) adventitious bud rooting
After cultivating 30-60d on culture medium three, jargonel adventitious bud is transferred to four (1/2MS+0.5-1mg/ of culture medium
LIBA+0.1-0.3mg/L PP333+ 0.3-0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar), material is put after inoculation
Enter culturing room and carry out normal condition culture, culturing room's condition of culture is：25 ± 2 DEG C, light application time 16h/d, light intensity 1500- of temperature
2500lx；
After cultivating 30-60d on culture medium four, jargonel adventitious bud rooting forms a large amount of test tube seedling.
Claims (1)
1. the method for early pears androgenesis, it is characterised in that there is following steps：(1) it in superclean bench, cuts
It takes early pears test tube seedling leaf and is inoculated in culture medium one：MS+2-3mg/L Thidiazuron+0.3-0.5mg/L IBA+0.3-
Material is put into culturing room after inoculation and carries out normal condition dark training by 0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar
It supports；(2) after cultivating 30-60d on culture medium one, early pears callus is transferred to culture medium two：MS+1-2mg/L Thidiazurons+
0.2-0.5mg/L IBA+0.3-0.5g/L carbon nanotube+0.3-0.5g/L graphene quantum dot+30g/L sucrose+7.5g/L fine jades
Material is put into culturing room after inoculation and carries out normal condition culture by fat；It (3), will be precocious after cultivating 30-60d on culture medium two
Pears simple bud is transferred to culture medium three：MS+1-2mg/L Thidiazuron+0.2-0.5mg/L IBA+0.3-0.5g/L carbon nanotubes+30g/L
Material is put into culturing room after inoculation and carries out normal condition culture by sucrose+7.5g/L agar；(4) 30- is cultivated on culture medium three
After 60d, jargonel adventitious bud is transferred to culture medium four：1/2MS+0.5-1mg/L IBA+0.1-0.3mg/L PP333+0.3-
Material is put into culturing room after inoculation and carries out normal condition culture by 0.5g/L carbon nanotube+30g/L sucrose+7.5g/L agar；
(5) after cultivating 30-60d on culture medium four, jargonel adventitious bud rooting forms a large amount of test tube seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810445952.2A CN108575755A (en) | 2018-05-03 | 2018-05-03 | A kind of method of morning pears androgenesis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810445952.2A CN108575755A (en) | 2018-05-03 | 2018-05-03 | A kind of method of morning pears androgenesis |
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| CN108575755A true CN108575755A (en) | 2018-09-28 |
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| CN201810445952.2A Pending CN108575755A (en) | 2018-05-03 | 2018-05-03 | A kind of method of morning pears androgenesis |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452156A (en) * | 2018-11-28 | 2019-03-12 | 上饶师范学院 | A method of improving early pears stem apex detoxification efficiency |
| CN111657147A (en) * | 2020-06-30 | 2020-09-15 | 富阿丽 | Differentiation culture medium and differentiation culture method for culture breeding of green Chinese onion flowers |
| CN112237142A (en) * | 2020-11-02 | 2021-01-19 | 江苏省中国科学院植物研究所 | Tissue culture medium for lycoris, callus culture method and method for establishing lycoris regeneration system |
| CN114532227A (en) * | 2022-03-07 | 2022-05-27 | 上海交通大学 | Method for inducing and proliferating calluses of agapanthus radicis roots and tips |
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| WO2011079212A2 (en) * | 2009-12-24 | 2011-06-30 | LifeSpan Extension, LLC | Methods and compositions for identifying, producing and using plant-derived products modulating cell function and aging |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452156A (en) * | 2018-11-28 | 2019-03-12 | 上饶师范学院 | A method of improving early pears stem apex detoxification efficiency |
| CN111657147A (en) * | 2020-06-30 | 2020-09-15 | 富阿丽 | Differentiation culture medium and differentiation culture method for culture breeding of green Chinese onion flowers |
| CN112237142A (en) * | 2020-11-02 | 2021-01-19 | 江苏省中国科学院植物研究所 | Tissue culture medium for lycoris, callus culture method and method for establishing lycoris regeneration system |
| CN112237142B (en) * | 2020-11-02 | 2022-04-26 | 江苏省中国科学院植物研究所 | Tissue culture medium for establishing Lycoris chinensis or lycoris aurea regeneration system and method thereof |
| CN114532227A (en) * | 2022-03-07 | 2022-05-27 | 上海交通大学 | Method for inducing and proliferating calluses of agapanthus radicis roots and tips |
| CN114532227B (en) * | 2022-03-07 | 2022-11-11 | 上海交通大学 | Method for inducing and proliferating calluses of agapanthus radicis roots and tips |
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