CN105794633A - Micro-cutting method for rapid propagation of Dalbergia odorifera seedlings - Google Patents
Micro-cutting method for rapid propagation of Dalbergia odorifera seedlings Download PDFInfo
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- CN105794633A CN105794633A CN201410826835.2A CN201410826835A CN105794633A CN 105794633 A CN105794633 A CN 105794633A CN 201410826835 A CN201410826835 A CN 201410826835A CN 105794633 A CN105794633 A CN 105794633A
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Abstract
The invention discloses a micro-cutting method for rapid propagation of Dalbergia odorifera seedlings. The method is as below: inoculating the seeds of Dalbergia odorifera into a bud induction culture medium and cultivating seedlings; cutting the obtained seedlings into stem segments, inserting the segments into a micro-cutting culture medium, and cultivating to grow lateral buds; cutting the lateral buds into stem segments, and inserting the stem segments into a fresh micro-cutting culture medium; cutting the obtained lateral buds into stem segments and inoculating the stem segments into a rooting culture medium, and cultivating for rooting, so as to obtain the Dalbergia odorifera seedlings. The process is simple and easy to operate, has rapid proliferation rate, resolves the problems of low germination rate and slow proliferation rate of seeds in seedlings breeding. The method achieves long-term, large-scale and rapid propagation of Dalbergia odorifera seedlings, can rapidly continuously obtain high-quality Dalbergia odorifera seedlings in a large amount and is practical.
Description
Technical field
The invention belongs to agricultural biological technical field, be specially a kind of method utilizing test tube stem section micro-cuttage Fast-propagation Dalbergia odorifera seedling.
Background technology
Dalbergia odorifera (Dalbergiaodorifera), endangered species, Hainan special product, the aiphyllium of pulse family Papillionoideae.Its timber is referred to as " Huanghua Pear ", is commonly called as " blood pressure lowering wood ", is Lignum Dalbergiae Odoriferae in Compendium of Material Medica, its wood flour soaked can blood pressure lowering, blood fat, its timber is wooden hard, and texture is beautiful, is the superior material making allusion hardwood furniture.Originate in the southern Chinese island of Hainan, be distributed mainly on the ground such as China Hainan, Guangdong, Fujian, two grades of Top-rated protected wild plants of country, endangered species.The heartwood of Dalbergia odorifera is most valuable, heartwood shows bronzing, dense materials is firmly heavy, have a fine grain attractive in appearance, self-assembling formation natural patterns, anticorrosive anti-wear, do not split and have a downwarp, and distribute fragrance, prolonged do not move back, be make high-grade redwood furniture, artware, musical instrument and engraving, inlay, the first-class material of art designing's decoration.Its timber is the Lignum Dalbergiae Odoriferae oil of gained after distillation, can make the fixastive on spice.Root heartwood and trunk heartwood can for Lignum Dalbergiae Odoriferaes, and hyoscine, for good analgesics.
The propagation method employing seminal propagation that Dalbergia odorifera is traditional, the method Problems existing: (1) seed resource is few, gathers difficulty in a large number；(2) seed not for shelf-stable, the life-span is short, it is impossible to realize regular seeding and seedling raising；
In recent years, cottage propagation and tissue culture propagation become the research emphasis of Dalbergia odorifera sapling multiplication.The existing problem of cottage propagation is: (1) cottage propagation survival rate is low, even all dead；(2) cuttage bar resource scarcity, batch breeds difficulty.Tissue culture propagation adopts the mode that callus induction, differentiation and proliferation callus and evoking adventive bud are taken root to obtain aseptic seedling at present, and this mode there is the problem that (1) callus induction time is longer；(2) Calli Differentiation is that the frequency of adventitious bud is relatively low, and the adventitious bud aberration rate differentiated is high；(3) the Elongation of adventitious bud speed that Calli Differentiation goes out is slow, and the time reaching the standard of taking root is long.These problems seriously hinder the large-scale production of Dalbergia odorifera seedling, thus cannot meet the market demand.For that purpose it is necessary to foundation energy is a large amount of, the production technology system of fast-propagation Dalbergia odorifera seedling.
Summary of the invention
A kind of method that it is an object of the invention to provide micro-cuttage Fast-propagation Dalbergia odorifera seedling, utilizes stem section sprouting of lateral bud and the fireballing feature of elongation in test tube, it is achieved that the purpose of the extensive fast-propagation of Dalbergia odorifera seedling.
The present invention utilizes a small amount of seed to obtain aseptic seedling, with aseptic seedling stem sections for outer implant, promotes that lateral bud is constantly sprouted by test tube micro-cuttage and carries out fast breeding, it is achieved Dalbergia odorifera seedling vast propagation.
The technical solution adopted in the present invention: a kind of method of micro-cuttage Fast-propagation Dalbergia odorifera seedling, including three processes: the acquisition of aseptic seedling, stem section micro-cuttage, stem section are taken root.
1, the acquisition of aseptic seedling
Choose the capsule of Dalbergia odorifera sterilized after seed is stripped out, seed is inoculated into and lures bud culture medium to carry out being cultured to growing up to seedling, described in lure bud culture medium be with MS culture medium for minimal medium additional 6-benzyladenine, α-naphthaleneacetic acid.
2, stem section micro-cuttage
Seedling step 1 obtained is cut into stem section, is downwardly in micro-cuttage culture medium by the base portion of stem section to carry out being cultured to and grows lateral bud, then lateral bud is cut into stem section, is then downwardly in fresh micro-cuttage culture medium by its base portion and cultivates.Described micro-cuttage culture medium is with MS culture medium for minimal medium additional 6-benzyladenine, heteroauxing.
3, stem section is taken root
Lateral bud step 2 obtained is cut into stem section, and is inoculated on root media, and first light culture carries out illumination cultivation again, and taking root to stem section to obtain Dalbergia odorifera seedling.The 1/2MS culture medium that described root media is improvement is minimal medium attached α-naphthaleneacetic acid.
Present invention process is simple, simple to operate, growth rate is fast, solve and utilize seed amount in Dalbergia odorifera seminal propagation seedling few, germination percentage is low, the problem that reproduction speed is slow, achieve the long-term extensive fast breeding of Dalbergia odorifera seedling, can be a large amount of, quickly, continue to obtain high-quality Dalbergia odorifera test tube Seedling, practical, efficient method is provided for Dalbergia odorifera vast propagation propagation, scale for Dalbergia odorifera seedling, factorial praluction is laid a good foundation, also for protecting wild Dalbergia odorifera germ plasm resource to open a practicable approach.
Detailed description of the invention
The invention will be further described below.
The method of micro-cuttage Fast-propagation Dalbergia odorifera seedling provided by the present invention, takes root including the acquisition of aseptic seedling, stem section micro-cuttage, stem section.
1) acquisition of aseptic seedling
Choose the capsule of Dalbergia odorifera sterilized after seed is stripped out, seed is inoculated into and lures bud culture medium to carry out being cultured to and grow up to seedling, cultivation temperature degree is 25～27 DEG C, and intensity of illumination is 15～20 μm of ol m-2·s-1, light application time is 12h d-1, cultivate 20～30 days；It is described that to lure bud culture medium be with MS culture medium for minimal medium additional 6-benzyladenine 0.5mg L-1, α-naphthaleneacetic acid 0.1mg L-1。
2) stem section micro-cuttage
Seedling step 1 obtained is cut into the stem section with 1～2 joint, it is downwardly in micro-cuttage culture medium by the base portion of stem section to carry out being cultured to growing lateral bud, lateral bud is cut into the stem section with 1～2 joint again, then its base portion is downwardly in fresh micro-cuttage culture medium and cultivates, cultivation temperature degree is 25～29 DEG C, and intensity of illumination is 30～40 μm of ol m-2·s-1, light application time is 12h d-1；Described micro-cuttage culture medium is with MS culture medium for minimal medium additional 6-benzyladenine 2.0mg L-1, heteroauxing 0.1mg L-1。
3) stem section is taken root
Lateral bud step 2 obtained is cut into the stem section with 1～2 joint, and is inoculated on root media, and first light culture carries out illumination cultivation 20～30 days in 3 days again, and taking root to stem section to obtain Dalbergia odorifera seedling.Described root media is the 1/2MS culture medium (1/2NH of improvement4NO3+1/2CaCl2) for minimal medium attached α-naphthaleneacetic acid 0.5mg L-1。
Claims (1)
1. a method for micro-cuttage Fast-propagation Dalbergia odorifera seedling, it is characterized in that and includes three processes: the acquisition of aseptic seedling, stem section micro-cuttage and stem section are taken root；
(1) acquisition of aseptic seedling
Choose the pod of Dalbergia odorifera sterilized after seed is stripped out, seed is inoculated into and lures bud culture medium to carry out being cultured to and grow up to seedling, cultivation temperature is 25～27 DEG C, and intensity of illumination is 16～20 μm of ol m-2·s-1, light application time is 12h d-1, cultivate 20～30 days；It is described that to lure bud culture medium be with MS culture medium for minimal medium additional 6-benzyladenine 0.5mg L-1, α-naphthaleneacetic acid 0.1mg L-1；
(2), stem section micro-cuttage
Seedling step 1 obtained is cut into the stem section with 1～2 joint, it is downwardly in micro-cuttage culture medium by the base portion of stem section to carry out being cultured to growing lateral bud, lateral bud is cut into the stem section with 1～2 joint again, then its base portion is downwardly in fresh micro-cuttage culture medium and cultivates, cultivation temperature is 25～29 DEG C, and intensity of illumination is 30～40 μm of ol m-2·s-1, light application time is 12h d-1；Described micro-cuttage culture medium is with MS culture medium for minimal medium additional 6-benzyladenine 2.0mg L-1, heteroauxing 0.1mg L-1；
(3), stem section is taken root
Lateral bud step 2 obtained is cut into the stem section with 1～2 joint, and is inoculated on root media, and first light culture carries out illumination cultivation 20～30 days in 3 days again, and taking root to stem section to obtain Dalbergia odorifera seedling；Described root media is the 1/2MS culture medium (1/2NH of improvement4NO3+1/2CaCl2) for minimal medium attached α-naphthaleneacetic acid 0.5mg L-1。
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718936A (en) * | 2017-02-14 | 2017-05-31 | 唐春艳 | A kind of yellow wingceltis tissue-cultured seedling rooting induction method |
| CN106718261A (en) * | 2016-11-24 | 2017-05-31 | 广西炎瑞农业发展有限公司 | A kind of Huanghua Pear culture medium and preparation method thereof |
| CN108703147A (en) * | 2018-06-29 | 2018-10-26 | 中国医学科学院药用植物研究所海南分所 | A kind of dalbergia odorifera plantation water-retaining agent, preparation method and applications |
| CN109452062A (en) * | 2018-10-10 | 2019-03-12 | 莆田市生物工程研究所 | A kind of dalbergia odorifera breeding nursery research method |
| CN109757375A (en) * | 2019-02-26 | 2019-05-17 | 中国林业科学研究院热带林业研究所 | A kind of tissue-cultured seedling subculture multiplication medium of dalbergia odorifera |
| CN109937725A (en) * | 2019-05-07 | 2019-06-28 | 浙江省亚热带作物研究所 | A kind of method of dalbergia odorifera softwood cutting propagation |
| CN112690212A (en) * | 2021-01-07 | 2021-04-23 | 中国林业科学研究院热带林业研究所 | Tissue culture method of dalbergia odorifera |
-
2014
- 2014-12-29 CN CN201410826835.2A patent/CN105794633A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718261A (en) * | 2016-11-24 | 2017-05-31 | 广西炎瑞农业发展有限公司 | A kind of Huanghua Pear culture medium and preparation method thereof |
| CN106718936A (en) * | 2017-02-14 | 2017-05-31 | 唐春艳 | A kind of yellow wingceltis tissue-cultured seedling rooting induction method |
| CN108703147A (en) * | 2018-06-29 | 2018-10-26 | 中国医学科学院药用植物研究所海南分所 | A kind of dalbergia odorifera plantation water-retaining agent, preparation method and applications |
| CN109452062A (en) * | 2018-10-10 | 2019-03-12 | 莆田市生物工程研究所 | A kind of dalbergia odorifera breeding nursery research method |
| CN109757375A (en) * | 2019-02-26 | 2019-05-17 | 中国林业科学研究院热带林业研究所 | A kind of tissue-cultured seedling subculture multiplication medium of dalbergia odorifera |
| CN109757375B (en) * | 2019-02-26 | 2021-11-09 | 中国林业科学研究院热带林业研究所 | Tissue culture seedling subculture multiplication medium for dalbergia odorifera |
| CN109937725A (en) * | 2019-05-07 | 2019-06-28 | 浙江省亚热带作物研究所 | A kind of method of dalbergia odorifera softwood cutting propagation |
| CN112690212A (en) * | 2021-01-07 | 2021-04-23 | 中国林业科学研究院热带林业研究所 | Tissue culture method of dalbergia odorifera |
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Application publication date: 20160727 |