CN108774631A - A kind of preparation method of rock kinases - Google Patents
A kind of preparation method of rock kinases Download PDFInfo
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- CN108774631A CN108774631A CN201811112255.1A CN201811112255A CN108774631A CN 108774631 A CN108774631 A CN 108774631A CN 201811112255 A CN201811112255 A CN 201811112255A CN 108774631 A CN108774631 A CN 108774631A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
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Abstract
The invention discloses a kind of preparation methods of rock kinases, including constitutive material and each raw material mass ratio be natural rock micro mist：Chelating agent：Complex enzyme：Pure water=1:0.05~0.1:0.1~0.5:1.5~3；The natural rock micro mist is made of following raw material according to mass percentage：Tourmaline stone micro mist 15% ~ 30%, germanite micro mist 15% ~ 25%, medical stone micro mist 15% ~ 35%, pelelith micro mist 20% ~ 35%, selenium ore micro mist 5% ~ 15%；Preparation process includes being prepared into natural rock micro mist under the conditions of high temperature and pressure Strong oxdiative after rock crushes, and pure water is added and chelating agent carries out chelatropic reaction, complex enzyme is added and is chelated, rock kinases is prepared.The present invention is by by complex enzyme and trace element chelated, being prepared the compound formulation of the trace element and enzyme of active high concentration, improving the functioning efficiency of enzyme.
Description
Technical field
The present invention relates to enzyme preparation technical fields, and in particular to a kind of preparation method of rock kinases.
Background technology
Enzyme preparation is widely used in food processing, feed, weaving, chemical industry, sewage disposal and agricultural etc. at present.
Industrial expansion causes very big pollution, and wherein cost of sewage disposal is very high, is extremely difficult to national standard, the reason is that it is difficult to by water
Molecule restores, even if using corresponding biological method such as processing with enzyme preparation sewage, it is dirty due to pollutant decomposition efficiency is low etc.
The effect of water process is poor.And agriculturally since soil long-term cultivation is unable to get recuperation, Trace Elements in Soil is serious
Missing, and single chemical fertilizer is used for a long time, balanced micro of the fruits and vegetables serious loss that the chemical fertilizer rich in elements such as N P and Ks is grown
Element, it is to inhibit insect pest using a large amount of pesticides to maintain this chemical fertilizer to promote the precondition of growth, causes vegetables and fruits pesticide residue super
Mark, can not reduce the product and method of pesticide residue effectively, using in relevant enzyme preparation degrading pesticide in the prior art
Noxious material when, degradation speed is slow, and degradation effect is poor.
Trace element is the indispensable part of institute in organism metabolic activity.For medical angle, trace element is
The missing of the supporting frame in body energy source, Trace Elements in Human Body can lead to iron-deficient heart disease, hypoferric anemia, leucoderma
Etc. diseases；Trace element is widely applied in medical product at present, such as 21 Jin Weita, multivitamin Centrum
Deng.But current research is usually limited only to how preferably to prepare trace element, is temporarily tied independent of between trace element and enzyme
Close the research of application.
1609205 A of patent CN disclose a kind of preparation method of degradation complex enzyme, select lignin peroxidase, phytic acid
Enzyme, phenol oxidase, formamidase, cyaniding hydrolase, formic acid glue hydrogen enzyme, lysozyme, tween solution, be mixed by sorting,
Dry, packaging and other steps are made, and realize degerming, decontamination, remove the works such as heavy metal pollution removing, degrading pesticide antibiotic hormone residues
With, but the type and content of enzyme are higher, and of high cost, enzymatic activity is relatively low, and reaction efficiency is relatively low.
Invention content
In order to solve existing technical problem, the present invention provides a kind of preparation method of rock kinases, by by complex enzyme
With it is trace element chelated, the trace element of active high concentration and the compound formulation of enzyme is prepared, improves the functioning efficiency of enzyme.
The technical scheme is that：A kind of preparation method of rock kinases, including constitutive material and each raw material quality
Than for natural rock micro mist：Chelating agent：Complex enzyme：Pure water=1: 0.05~0.1 : 0.1~0.5 : 1.5~3；It is described natural
Rock micro mist is made of following raw material according to mass percentage：Tourmaline stone micro mist 15% ~ 30%, germanite micro mist 15% ~ 25%, wheat
Meal stone micro mist 15% ~ 35%, pelelith micro mist 20% ~ 35%, selenium ore micro mist 5% ~ 15%；
Preparation process is as follows：
（1）Tourmaline stone, germanite, medical stone, pelelith, selenium ore are ground into 1000 mesh with down toward nanoscale, difference respectively
It is prepared into the micro mist of various rocks, according to the constitutive material and mass percent of natural rock micro mist, it is micro- to weigh corresponding rock
Powder is put into pyroreaction kettle, temperature is 120 DEG C, pressure is 2 × 105It is reacted under the conditions of the high temperature and pressure Strong oxdiative of Pa
5h forms natural rock micro mist；
（2）By step（1）In natural rock micro mist cooled to room temperature, then be added pure water, chelating agent, be in temperature
Under conditions of 28 DEG C, chelatropic reaction is carried out for 24 hours；
（3）In step（2）After middle chelatropic reaction, then complex enzyme is added in cooled to room temperature into pyroreaction kettle,
It stirs evenly, carries out chelatropic reaction for 24 hours under normal temperature condition, rock kinases is prepared.
Preferably, the mass ratio for the constitutive material and each raw material that the rock kinases includes is natural rock micro mist：Chelating agent：
Complex enzyme：Pure water=1:0.08:0.3:2.
Preferably, the natural rock micro mist is made of following raw material according to mass percentage：Tourmaline stone micro mist
20%, germanite micro mist 20%, medical stone micro mist 25%, pelelith micro mist 25%, selenium ore micro mist 10%.
Preferably, the complex enzyme is one kind in animal protease or plant complex enzyme.
Preferably, the complex enzyme is organic phosphorus degrading enzyme, carbamatic hydrolase and pyrethroid ester hydrolase
Mixture.
In a kind of preparation method of rock kinases of the present invention, Tourmaline stone contains whole macroelements of animal needs, such as
K, Na, Ca, Mg etc., the trace element such as Cu, Mo and rare earth element, share 50 various trace elements.A large amount of germanium is to divide in germanite
Bulk state is present in the silicate mine, sulfide mine and various types of coals of various metals.Medical stone contains needed for animal
Whole macroelements, if K, Na, Ca, Mg and Cu, Mo etc. trace element and rare earth element, as many as about 58 kinds.Contain in pelelith
There are tens kinds of minerals such as Na, Mg, Al, Si, Ca, Ti, Mn, Fe, Ni, Co, Mo and trace element.Rich in Se members in selenium ore
Element.Contain abundant trace element in natural rock, by being prepared into natural rock micro mist, and water mixing be added, with chelating agent,
After complex enzyme chelating, the micro- cell activity that can remarkably promote enzyme, fast and effeciently microelement-supplementing adjust body
It is balanced.The high catalytic efficiency of rock kinases can use plant complex enzyme that can use animal protein for animal for plant
Enzyme.Rock kinases prepared by the present invention can be widely applied to medicine, health care, beauty, improvement soil, environmental protection, sewage are controlled
Reason, plantation cultivation etc..In terms of sewage disposal, the Equilibrium of Trace Elements water quality in rock kinases, and promote the growth of enzyme, soon
Speed decomposes the pollutant in water, to effectively administer sewage.In terms of planting cultivation, while using rock kinases, it can continue
Using pesticide, rock kinases, which is mainly combined by enzyme with chemistry of pesticide molecule, is converted into other innocuous substances, to agriculture of effectively degrading
Medicine remains.By taking organic phosphorus degrading enzyme as an example, organic phosphorus degrading enzyme by cut off noxious material in P-O keys, P-F keys, P-S keys,
Macromolecular moment toxic, not soluble in water is degraded into small molecule that is nontoxic, being dissolved in water and will not cause two to environment by P-CN keys
Secondary pollution is a kind of nontoxic, efficient, environmentally friendly biological high-tech product.Organic phosphorus degrading enzyme is added in some noxious materials,
Its degradation speed can be made to improve 1000 to 2450 times；And rock kinases is the accelerating agent of organic phosphorus degrading enzyme, can quickly be supplemented micro-
Secondary element promotes organic phosphorus degrading enzyme and chemistry of pesticide point by the neutralization of acid-base neutralization reaction, trace element and trace element
Son combines and is converted into other innocuous substances, promotes the growing of crops, effective degrading pesticide residues.
Using having the beneficial effect that for above-mentioned technical proposal：
（1）The present invention is by complex enzyme and trace element chelated, and fast and effeciently microelement-supplementing, improves the work of rock kinases
Property, improve the functioning efficiency of rock kinases.
（2）Animal protease is used for animal in the present invention, plant complex enzyme, the rock being prepared are used for plant
Kinases is widely used in the fields such as medicine, environmental improvement, plantation cultivation；It, can when especially rock kinases is used to plant cultivation field
It effectively degrades remaining pesticide.
（3）Preparation method is simple, easily operated, and production cost is low, can be widely applied to produce.
Specific implementation mode
With reference to specific embodiment, the present invention is described in further detail.
Embodiment 1
A kind of preparation method of rock kinases, including constitutive material and the quality of each raw material be：Natural rock micro mist 100g, chelating
Agent 8g, complex enzyme 30g, pure water 200g；The natural rock micro mist is made of the quality of following raw material and raw material：Tourmaline stone
Micro mist 20g, germanite micro mist 20g, medical stone micro mist 25g, pelelith micro mist 25g, selenium ore micro mist 10g；
Preparation process is as follows：
（1）Tourmaline stone, germanite, medical stone, pelelith, selenium ore are ground into 1000 mesh with down toward nanoscale, difference respectively
It is prepared into the micro mist of various rocks, according to the constitutive material and quality of natural rock micro mist, weighs corresponding rock micro mist, is put into
In pyroreaction kettle, temperature is 120 DEG C, pressure is 2 × 1055h is reacted under the conditions of the high temperature and pressure Strong oxdiative of Pa, forms day
Right rock micro mist；
（2）By step（1）In natural rock micro mist cooled to room temperature, 200g pure water, 8g chelating agents is then added,
Under conditions of temperature is 28 DEG C, chelatropic reaction is carried out for 24 hours；
（3）In step（2）After middle chelatropic reaction, then it is compound that 30g is added in cooled to room temperature into pyroreaction kettle
Enzyme stirs evenly, and carries out chelatropic reaction for 24 hours under normal temperature condition, rock kinases is prepared.
The complex enzyme is the mixture of organic phosphorus degrading enzyme, carbamatic hydrolase and pyrethroid ester hydrolase.
The rock kinases of the present embodiment is applied to plantation cultivation field, for example, tealeaves, radish, eggplant, leek, shallot kind
It plants, normal use pesticide in planting process, and uses rock kinases simultaneously, quality inspection, quality inspection are carried out to the product after maturation
Test commission Ministry of Agriculture's food quality supervision verification test center（Shanghai）It carries out, inspection result shows that all products do not have
Pesticide residue, by taking tealeaves and eggplant as an example, specific inspection result is as follows：
（1）Shown in tea quality inspection result chart 1：
1 tealeaves inspection result report of table
According to table 1 it is found that carrying out Tea planting using rock kinases, obtained tea products do not have pesticide residue, illustrate rock kinases
The pesticide residue that the Tea planting that can effectively degrade uses in the process.
（2）Shown in eggplant quality test results chart 2：
2 eggplant inspection result report of table
According to table 2 it is found that carrying out eggplant plantation using rock kinases, obtained product does not have pesticide residue, illustrates that rock kinases can have
The pesticide used in effect degradation eggplant planting process.
In summary content and inspection result be it is found that in planting breeding process, using using rock while different pesticides
Kinases, can quick microelement-supplementing, promote crop growth, and remaining pesticide of degrading.
Claims (5)
1. a kind of preparation method of rock kinases, it is characterised in that：Including constitutive material and each raw material mass ratio be natural rock
Stone micro mist：Chelating agent：Complex enzyme：Pure water=1: 0.05~0.1 : 0.1~0.5 : 1.5~3；The natural rock micro mist is pressed
It is made of following raw material according to mass percentage：Tourmaline stone micro mist 15% ~ 30%, germanite micro mist 15% ~ 25%, medical stone micro mist
15% ~ 35%, pelelith micro mist 20% ~ 35%, selenium ore micro mist 5% ~ 15%；
Preparation process is as follows：
（1）Tourmaline stone, germanite, medical stone, pelelith, selenium ore are ground into 1000 mesh with down toward nanoscale, difference respectively
It is prepared into the micro mist of various rocks, according to the constitutive material and mass percent of natural rock micro mist, it is micro- to weigh corresponding rock
Powder is put into pyroreaction kettle, temperature is 120 DEG C, pressure is 2 × 105It is reacted under the conditions of the high temperature and pressure Strong oxdiative of Pa
5h forms natural rock micro mist；
（2）By step（1）In natural rock micro mist cooled to room temperature, then be added pure water, chelating agent, be in temperature
Under conditions of 28 DEG C, chelatropic reaction is carried out for 24 hours；
（3）In step（2）After middle chelatropic reaction, then complex enzyme is added in cooled to room temperature into pyroreaction kettle,
It stirs evenly, carries out chelatropic reaction for 24 hours under normal temperature condition, rock kinases is prepared.
2. a kind of preparation method of rock kinases as described in claim 1, it is characterised in that：The composition that the rock kinases includes is former
The mass ratio of material and each raw material is natural rock micro mist：Chelating agent：Complex enzyme：Pure water=1:0.08:0.3:2.
3. a kind of preparation method of rock kinases as described in claim 1, it is characterised in that：The natural rock micro mist is according to matter
Amount percentage meter is made of following raw material：Tourmaline stone micro mist 20%, germanite micro mist 20%, medical stone micro mist 25%, pelelith micro mist
25%, selenium ore micro mist 10%.
4. a kind of preparation method of rock kinases as described in claim 1, it is characterised in that：The complex enzyme is animal protease
Or one kind in plant complex enzyme.
5. a kind of preparation method of rock kinases as described in claim 1 or 4, it is characterised in that：The complex enzyme is organophosphor
The mixture of degrading enzyme, carbamatic hydrolase and pyrethroid ester hydrolase.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811112255.1A CN108774631B (en) | 2018-09-25 | 2018-09-25 | A kind of preparation method of rock kinases |
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| CN201811112255.1A CN108774631B (en) | 2018-09-25 | 2018-09-25 | A kind of preparation method of rock kinases |
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| CN108774631A true CN108774631A (en) | 2018-11-09 |
| CN108774631B CN108774631B (en) | 2019-01-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111803518A (en) * | 2020-07-22 | 2020-10-23 | 烟台冠玉生物科技有限公司 | Liquid trace element, preparation method and application |
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| CN101156659A (en) * | 2007-11-12 | 2008-04-09 | 河南三农生态产业有限公司 | Organic swine core premix |
| CN104829272A (en) * | 2009-12-22 | 2015-08-12 | 拉尔科营养品有限公司 | Organically chelated mineral compositions and methods thereof |
| CN105053726A (en) * | 2015-09-11 | 2015-11-18 | 安徽科技学院 | Fodder capable of stimulating appetite of pheasant for table purpose |
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2018
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156659A (en) * | 2007-11-12 | 2008-04-09 | 河南三农生态产业有限公司 | Organic swine core premix |
| CN104829272A (en) * | 2009-12-22 | 2015-08-12 | 拉尔科营养品有限公司 | Organically chelated mineral compositions and methods thereof |
| CN105053726A (en) * | 2015-09-11 | 2015-11-18 | 安徽科技学院 | Fodder capable of stimulating appetite of pheasant for table purpose |
Non-Patent Citations (3)
| Title |
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| PILON CRISTIANE等: "Effects of soil and foliar application of soluble silicon on mineral nutrifion，gas exchange，and growth of potato plants", 《CROP SCIENCE》 * |
| 王冠等: "不同载体性质及对植酸酶活性的影响", 《国外畜牧学-猪与禽》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111803518A (en) * | 2020-07-22 | 2020-10-23 | 烟台冠玉生物科技有限公司 | Liquid trace element, preparation method and application |
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