CN108771636A - 一种溶菌酶制剂 - Google Patents
一种溶菌酶制剂 Download PDFInfo
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- CN108771636A CN108771636A CN201810918495.4A CN201810918495A CN108771636A CN 108771636 A CN108771636 A CN 108771636A CN 201810918495 A CN201810918495 A CN 201810918495A CN 108771636 A CN108771636 A CN 108771636A
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- chitosan
- lysozyme
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Abstract
本发明涉及到一种溶菌酶制剂,包括溶酶菌A试剂,所述溶酶菌A试剂中有效成分为溶菌酶提纯液与硅油在高速搅拌下形成的溶酶菌‑壳聚糖‑硅油。将其混入其他护肤品组分中涂抹在皮肤上时,硅油大分子因自身分子量大以及空间位阻的情况无法从毛孔深入皮肤深处进行清洁,因此在皮肤表面形成均匀的水膜,壳聚糖作为载体,对连接在其分子链表面的溶酶菌有良好的缓释作用;一部分壳聚糖与硅油大分子一同覆盖在皮肤表面,另一部分位于毛孔处即毛孔中的溶菌酶和皮肤表面的硅油分子之间,溶酶菌小分子从毛孔处深入进皮肤中,起到深层净化皮肤和人体内环境的作用,之后壳聚糖释放完所有的溶菌酶后对毛孔缩小有一定的作用效果。
Description
技术领域
本发明涉及一种溶菌酶,特别涉及一种溶菌酶制剂。
背景技术
目前化妆品种类繁多,其作用、效果也大有不同,例如祛斑、祛痘、美白、修复等;但是这些美容产品大多是治标不治本,无法真正的从根源去除皮肤表面的色斑、闭口、痘痘等,而究其根源,则是因为人体内部经络不通、血液淤堵,从而导致肤色暗沉、体虚肥胖、免疫功能下降等情况。
溶菌酶(lysozyme)又称胞壁质酶(muramidase)或N-乙酰胞壁质聚糖水解酶(N-acetylmuramide glycanohydrlase),是一种能水解致病菌中黏多糖的碱性酶。主要通过破坏细胞壁中的N-乙酰胞壁酸和N-乙酰氨基葡糖之间的β-1,4糖苷键,使细胞壁不溶性黏多糖分解成可溶性糖肽,导致细胞壁破裂内容物逸出而使细菌溶解。溶菌酶还可与带负电荷的病毒蛋白直接结合,与DNA、RNA、脱辅基蛋白形成复盐,使病毒失活。因此,该酶具有抗菌、消炎、抗病毒等作用。
目前,将溶菌酶添加在化妆品中能对部分皮肤问题有效改善,例如各类斑的去除,如申请号为“201510414877.X”的中国专利所公开的一种人溶菌酶精华液,对皮肤具有明显的美白滋润、护肤美容的效果。
例如国际公布号为“WO2006/079288A1”的PCT国际专利所公开的一种人溶菌酶在制备治疗痤疮的化妆品中的应用,可有效用于痤疮的治疗。
综上所述,溶菌酶在化妆品领域的添加应用已然成问一种全新的领域,也为化妆品制造提供了一种全新的思路。
本发明公开一共溶菌酶制剂,对人体具有良好的净化效果,使涂覆在皮肤上的化妆品发挥出更大的功效。
发明内容
本发明的目的是提供一种溶菌酶制剂。
本发明的上述技术目的是通过以下技术方案得以实现的:一种溶菌酶制剂,包括溶酶菌A试剂,所述溶酶菌A试剂中有效成分为溶菌酶提纯液与硅油在高速搅拌下形成的溶酶菌-壳聚糖-硅油。
作为优选,还包括以下组分,以下组分按照重量计:
作为优选,所述溶酶菌-壳聚糖-硅油的制备方法主要包括以下步骤:
步骤1,蛋液分离:去除蛋黄,去除杂质;
步骤2,预备液制备,将二氧化硅作为制孔剂,混入高分子壳聚糖中,制备得到壳聚糖多孔膜:将壳聚糖溶解在低浓度的乙酸溶液中形成壳聚糖-乙酸溶液,将壳聚糖-乙酸溶液逐滴添加至液体石蜡中,滴加过程中持续搅拌,滴加完毕后高速搅拌至乳化;将低浓度戊二醛溶液与碱性二氧化硅混合并逐滴滴加至混合溶液中,搅拌至完全混合后,离心过滤5-8min,去除上层清液,将下层淡黄色沉淀收集,多次洗涤得到纯净物后,冻干研磨成粉;
步骤3:将预备液体与步骤1中制备的蛋液在微酸性环境下混合,静置24-48h后,离心8-15分钟后去除上层清液取下层沉淀;
步骤4:将卤代硅烷与有机溶剂混合后逐滴滴加至步骤3中下层沉淀中,搅拌并升温至50-58℃,搅拌10-20h后,减压蒸馏/减压干燥得到高浓度溶酶菌-壳聚糖-硅油复合物质;
步骤5:多次洗涤后收集。
作为优选,所述步骤3中微酸环境为偏磷酸钠水溶液,所述步骤4中有机溶剂为DMA。
作为优选,还包括辅助制剂A,所述辅助制剂A包括青刺果油提取物、玫瑰花油提取物、白兰花油提取物;所述溶菌酶A试剂与辅助制剂A混合形成净化精油。
作为优选,还包括辅助制剂B,所述辅助制剂B包括葛根提取物、大豆异黄酮提取物、植物胎盘液;所述辅助制剂B与溶菌酶A制剂混合形成净化精华。
作为优选,还包括辅助制剂C,所述辅助制剂C包括蜂胶提取物、薄荷脑、尿囊素;所述辅助制剂C和溶菌酶A制剂混合形成净化霜。
作为优选,将所述净化精油、净化净化或净化霜均匀涂抹在身体淋巴系统所在位置后配合按摩手法按摩直至吸收。
综上所述,本发明具有以下有益效果:
1.本方案中的有效成为溶酶菌-壳聚糖-硅油,将其混入其他护肤品组分中涂抹在皮肤上时,硅油大分子因自身分子量大以及空间位阻的情况无法从毛孔深入皮肤深处进行清洁,因此在皮肤表面形成均匀的水膜,壳聚糖作为载体,对连接在其分子链表面的溶酶菌有良好的缓释作用;一部分壳聚糖与硅油大分子一同覆盖在皮肤表面,另一部分位于毛孔处即毛孔中的溶菌酶和皮肤表面的硅油分子之间,溶酶菌小分子从毛孔处深入进皮肤中,起到深层净化皮肤和人体内环境的的作用,之后壳聚糖释放完所有的溶菌酶后对毛孔缩小有一定的作用效果;
2.目前,溶酶菌的来源主要是鸡蛋清溶液中的溶菌酶多肽链,主要由129个氨基酸残基组成,包括6个色氨基残基、3个络氨酸残基和4对二硫键,以上三种基团的存在决定了溶酶菌的活性;而现有技术中对溶酶菌的制备提纯主要方法为直接结晶法、粒子交换法和亲和层析法,以上几种制备方法工艺复杂、成本较高,且对其上几种影响溶酶菌活性的残基的保留率有所影响;而本方案中通过温和的吸附手段取得鸡蛋清中的溶酶菌,极大程度上保留了溶酶菌的活性,且制备、提纯成本相对较低,操作简单易实施;
3.将硅油-溶酶菌-壳聚糖与角鲨烷、透明质酸、氨基酸、维生素B1、玻尿酸等混合,去离子水帮助溶解和在溶剂中的均匀分布,表面活性剂促使不溶物质溶解;以上几种均对皮肤有良好的保湿、补水、修复等功效,微生物B1帮助皮肤毛孔打开溶酶菌进入,角鲨烷等可帮助溶酶菌作用完毕后重新修复皮肤,使皮肤容光焕发;
4.将本方案中的溶菌酶A试剂分别添加至辅助制剂A、辅助制剂B、辅助制剂C中形成净化精油、净化净化、净化霜;将其涂抹在皮肤表面并配合按摩手法,有助于皮肤对以上物质的吸收,可加速经络循环代写、放松肌肉、安抚背部疲劳、疏通乳房对应反射经络,预防因置放堆积形成的背部变形;净化净化涂抹在胸部可激活乳房内腺体、腺泡,补充乳房内需要的大量营养和水分,达到丰盈饱满的效果;净化霜具有疏通淋巴、改善淋巴淤堵,给淋巴系统输送动能,增强NK,LAK细胞的或于都,有效促进淋巴循环代谢预防疾病。
具体实施方式
本具体实施例仅仅是对本发明的解释,其并不是对本发明的限制,本领域技术人员在阅读完本说明书后可以根据需要对本实施例做出没有创造性贡献的修改,但只要在本发明的权利要求范围内都受到专利法的保护。
实施例:
一种溶菌酶制剂,主要包括溶酶菌-硅油-壳聚糖,制备提纯方法如下所示:
步骤1,蛋液分离:去除蛋黄,去除杂质;
步骤2,预备液制备,将二氧化硅作为制孔剂,混入高分子壳聚糖中,制备得到壳聚糖多孔膜:将壳聚糖溶解在低浓度的乙酸溶液中形成壳聚糖-乙酸溶液,将壳聚糖-乙酸溶液逐滴添加至液体石蜡中,滴加过程中持续搅拌,滴加完毕后高速搅拌至乳化;将低浓度戊二醛溶液与碱性二氧化硅混合并逐滴滴加至混合溶液中,搅拌至完全混合后,离心过滤5-8min,去除上层清液,将下层淡黄色沉淀收集,多次洗涤得到纯净物后,冻干研磨成粉;
步骤3:将预备液体与步骤1中制备的蛋液在微酸性环境下混合,静置24-48h后,离心8-15分钟后去除上层清液取下层沉淀;利用偏磷酸钠营造微酸性环境;
步骤4:将卤代硅烷与有机溶剂混合后逐滴滴加至步骤3中下层沉淀中,搅拌并升温至50-58℃,搅拌10-20h后,减压蒸馏/减压干燥得到高浓度硅油-溶酶菌-壳聚糖复合物质,有机溶剂采用DMA;
步骤5:多次洗涤后收集。
溶菌酶A试剂中还包括角鲨烷、透明质酸、氨基酸、维生素B1、表面活性剂、玻尿酸、去离子水,实施例1-实施例5中各物质组成相同但具体含量不同,详见下表1所示;
表1:
将实施例1-实施例5中制备得到溶酶菌A试剂分别添加至辅助制剂A、辅助制剂B和辅助制剂C中形成净化精油、净化精华和净化霜。
辅助制剂A包括:青刺果油提取物、玫瑰花油提取物、白兰花油提取物。
辅助制剂B包括:葛根提取物、大豆异黄酮提取物、植物胎盘液。
辅助制剂C包括:蜂胶提取物、薄荷脑、尿囊素。
辅助制剂A、辅助制剂B和辅助制剂C中各物质的比例关系为1:1:1,溶菌酶A试剂的添加量为25%-50%。
将制备得到的净化精油、净化净化或净化霜均匀涂抹在身体淋巴系统所在位置后配合按摩手法按摩直至吸收,持续涂抹1-3疗程后,皮肤状态有效改善。
Claims (8)
1.一种溶菌酶制剂,其特征在于,包括溶酶菌A试剂,所述溶酶菌A试剂中有效成分为溶菌酶提纯液与硅油在高速搅拌下形成的溶酶菌-壳聚糖-硅油。
2.根据权利要求1所述的一种溶菌酶制剂,其特征在于,还包括以下组分,以下组分按照重量计:
3.根据权利要求2所述的一种溶菌酶制剂,其特征在于,所述溶酶菌-壳聚糖-硅油的制备方法主要包括以下步骤:
步骤1,蛋液分离:去除蛋黄,去除杂质;
步骤2,预备液制备,将二氧化硅作为制孔剂,混入高分子壳聚糖中,制备得到壳聚糖多孔膜:将壳聚糖溶解在低浓度的乙酸溶液中形成壳聚糖-乙酸溶液,将壳聚糖-乙酸溶液逐滴添加至液体石蜡中,滴加过程中持续搅拌,滴加完毕后高速搅拌至乳化;将低浓度戊二醛溶液与碱性二氧化硅混合并逐滴滴加至混合溶液中,搅拌至完全混合后,离心过滤5-8min,去除上层清液,将下层淡黄色沉淀收集,多次洗涤得到纯净物后,冻干研磨成粉;
步骤3:将预备液体与步骤1中制备的蛋液在微酸性环境下混合,静置24-48h后,离心8-15分钟后去除上层清液取下层沉淀;
步骤4:将卤代硅烷与有机溶剂混合后逐滴滴加至步骤3中下层沉淀中,搅拌并升温至50-58℃,搅拌10-20h后,减压蒸馏/减压干燥得到高浓度溶酶菌-壳聚糖-硅油复合物质;
步骤5:多次洗涤后收集。
4.根据权利要求3所述的一种溶菌酶制剂,其特征在于:所述步骤3中微酸环境为偏磷酸钠水溶液,所述步骤4中有机溶剂为DMA。
5.根据权利要求4所述的一种溶菌酶制剂,其特征在于:还包括辅助制剂A,所述辅助制剂A包括青刺果油提取物、玫瑰花油提取物、白兰花油提取物;所述溶菌酶A试剂与辅助制剂A混合形成净化精油。
6.根据权利要求5所述的一种溶菌酶制剂,其特征在于:还包括辅助制剂B,所述辅助制剂B包括葛根提取物、大豆异黄酮提取物、植物胎盘液;所述辅助制剂B与溶菌酶A制剂混合形成净化精华。
7.根据权利要求6所述的一种溶菌酶制剂,其特征在于:还包括辅助制剂C,所述辅助制剂C包括蜂胶提取物、薄荷脑、尿囊素;所述辅助制剂C和溶菌酶A制剂混合形成净化霜。
8.根据权利要求7所述的一种溶菌酶制剂,其特征在于:将所述净化精油、净化净化或净化霜均匀涂抹在身体淋巴系统所在位置后配合按摩手法按摩直至吸收。
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