CN108753611B

CN108753611B - Soft tissue digestion device for primary cell culture

Info

Publication number: CN108753611B
Application number: CN201810582871.7A
Authority: CN
Inventors: 李华涛
Original assignee: Qingdao Agricultural University
Current assignee: Qingdao Agricultural University
Priority date: 2018-06-06
Filing date: 2018-06-06
Publication date: 2023-03-24
Anticipated expiration: 2038-06-06
Also published as: CN108753611A

Abstract

The invention discloses a soft tissue digestion device for primary cell culture, which comprises a base, a digestion vessel, a spherical stirring rotor, a glass ball and a rotor traction disc, wherein the rotor traction disc is positioned in the base and is driven to rotate by a motor; the rotor traction disc comprises blades arranged in a crossed manner, magnets are arranged on each blade at equal intervals, the distance between every two adjacent magnets is larger than the diameter of the glass ball, and the magnets on the two blades are arranged in a crossed manner; the spherical stirring rotor comprises an iron core and a ceramic layer wrapped outside the iron core; after the spherical stirring rotor is placed in the digestion vessel, the spherical stirring rotor is adsorbed by the corresponding magnet; a digestion vessel placing area is arranged on the upper surface of the base, and the blades are located below the digestion vessel placing area. The invention does not need to shake at corresponding time intervals, and the digestion time is shorter than that of the prior method, and the cells after digestion can be inoculated only by washing without manually blowing and beating.

Description

Soft tissue digestion device for primary cell culture

Technical Field

The invention relates to a soft tissue digestion device for primary cell culture.

Background

In human or animal body (or embryo tissue), due to the tight combination of multiple cells, it is not favorable for the growth and reproduction of each cell in vitro culture, even if 1mm is adopted ³ The tissue mass of (a), only a few cells in the periphery may survive and grow, if a large number of cells need to be obtained, the existing tissue mass must be fully dispersed to dissociate the cells, and the currently adopted method is as follows:

shearing the tissue into small blocks (or paste), loosening intercellular bridging structure by biochemical action of enzyme and chemical action of nonenzymatic enzyme to make the blocks become flocculent, and mechanically dispersing or electromagnetically stirring by sucking tube or oscillating in bead-shaking bottle to fully disperse cell blocks and obtain cell suspension containing a small amount of cell blocks and a large amount of single cells.

However, the above method has the following disadvantages:

1. the collagenase is required to be fully contacted with the tissues by shaking once every 5 minutes or once every 30 minutes, so that the effect of rapid digestion is achieved. Therefore, the operation of workers at corresponding time intervals is required, and the workload is large.

2. The digestive juice is properly blown and sucked by the gun head manually, so that the cell mass and the undigested and complete tissue mass which are stuck together are separated. Human factors have a great influence.

3. The individual types of tissue cells are digested for a longer time, increasing the damage to the cells by digestive enzymes.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides the soft tissue digestion device for primary cell culture, which does not need to vibrate at corresponding intervals, shortens the digestion time compared with the prior method, and can inoculate cells only by washing without manually blowing and beating the digested cells.

The technical scheme adopted by the invention for solving the technical problem is as follows: a soft tissue digestion device for primary cell culture, which comprises a base, a digestion vessel, a spherical stirring rotor, a glass ball and a rotor traction disc,

the rotor traction disc is positioned in the base and is driven to rotate by a motor; the rotor traction disc comprises crossed blades, magnets are arranged on each blade at equal intervals, the distance between every two adjacent magnets is larger than the diameter of the glass ball, and the magnets on the two blades are arranged in a crossed mode;

the spherical stirring rotor comprises an iron core and a ceramic layer wrapped outside the iron core; after the spherical stirring rotors are placed in the digestion vessel, the spherical stirring rotors are adsorbed by the corresponding magnets;

the upper surface of the base is provided with a digestion vessel placing area, and the blades are positioned below the digestion vessel placing area.

Furthermore, the surface of the glass ball is a frosted surface.

Further, a blade indicator of the rotor traction disc is arranged in the digestion vessel placing area, and a spherical stirring rotor position point is drawn on the blade indicator; and in the static state of the rotor traction disc, the magnets on the blades correspond to the position points.

Furthermore, a controller for controlling the motor is arranged on the base, and the motor can be operated by the controller to work at regular time and interval time.

The invention has the beneficial effects that: the device saves time and labor, is uniform in digestion and has small damage to cells. Can drive spherical stirring rotor when rotor traction disc rotates and rotate, promote the interior glass ball of digestion ware and remove when the rotor removes, reach the effect of dispersion soft tissue piece through extrusion between the glass pearl and grinding, and then fast get to make the tissue piece digest into the unicell.

The device does not need to oscillate at intervals of corresponding time, and can set the working time of the motor to work at intervals. The digestion time is short, and the working efficiency is improved. After digestion, the cells can be inoculated only by washing without manually blowing.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention (not shown with a protective film);

FIG. 2 is a schematic view of the base structure of the present invention;

FIG. 3 is a schematic view of the arrangement of the spherical stirring rotor and glass spheres of the present invention;

FIG. 4 is a schematic diagram of the position of the magnet after the two blades are overlapped;

FIG. 5 is a cross-sectional view of a spherical mixing rotor of the present invention;

in the figure, a base 1, a digestion vessel placing area 11, a blade indicator 12, a position point 13, a control key 14, a display screen 15, a digestion vessel 2, a cover 21, a spherical stirring rotor 3, an iron core 31, a ceramic layer 32, a glass ball 4, a rotor traction disc 5, a blade 51, a magnet 52 and a motor 6.

Detailed Description

For a better understanding of the present invention, embodiments thereof are explained in detail below with reference to the accompanying drawings.

As shown in fig. 1 to 4, the present invention comprises a base 1, a digestion vessel 2, a ball-shaped stirring rotor 3, glass balls 4 and a rotor traction disc 5. In order to ensure the friction effect, the surface of the glass ball 4 is a frosted surface.

The rotor traction disc 5 is located in the base 1, as shown in fig. 1, the rotor traction disc 5 is connected with an output shaft of the motor 6 through a rotating shaft, and the rotor traction disc 5 is driven to rotate through the motor 6. The blades 51 of the rotor traction disk 5 are adjacent to the bottom surface of the upper surface of the base 1. As shown in fig. 3, the rotor traction disk 5 includes blades 51 arranged crosswise, and the two blades 51 are fixedly connected. Magnets 52 are provided at equal intervals on each of the blades 51, and as shown in fig. 1, the magnets 52 are sunk in the blades 51. The distance between two adjacent magnets 52 is larger than the diameter of the glass ball 4, so that the glass ball 4 can smoothly pass through the space between the two spherical stirring rotors 3. As shown in fig. 3, the magnets 52 of the two blades 51 are arranged to intersect, and if the two blades 51 are overlapped, the magnets 52 of the two blades 51 are staggered as shown in fig. 4.

As shown in FIG. 5, in order to prevent the cells from being damaged by the machine and form a better friction surface with the glass spheres, the spherical stirring rotor 3 comprises an iron core 31 and a ceramic layer 32 wrapped outside the iron core 31, the thickness of the ceramic layer 32 is only required to be about 0.5-2mm, and the surface of the ceramic layer 32 can also be a frosted surface.

After the spherical stirring rotors 3 are placed in the digestion vessel 2, the spherical stirring rotors 3 are adsorbed by the corresponding magnets 52 on the blades 51, and each magnet 52 adsorbs one spherical stirring rotor 3. The rotation of the blade 51 drives the rotation of the magnet 52.

A digestion vessel placing area 11 is arranged on the upper surface of the base 1, the blades 51 are positioned below the digestion vessel placing area 11, and the upper surface of the base 1 is a non-iron plate with the thickness of about 0.5-1mm, preferably a 0.5mm plastic plate. A blade indicator 12 of the rotor traction disk 5 is arranged in the digestion vessel placing area 11, and a position point 13 of a spherical stirring rotor is drawn on the blade indicator 12. In the rest state of the rotor traction disk 5, the magnet 52 on the blade 51 corresponds to the position point 13.

In order to flexibly control the digestion time and the digestion mode of cell tissues, a controller for controlling the motor 6 is arranged on the base 1, a control key 14 and a display screen 15 are arranged on the base 1, and the motor 6 can be operated to work at fixed time and at intervals by setting the timing and the working mode (continuous working or intermittent working) through the control key.

When the device is used, the digestion vessel 2 is placed in the digestion vessel placing area 11, the digestion vessel 2 is made of transparent glass, then the spherical stirring rotor 3 is correspondingly placed according to the position point 13, and finally the bottom of the digestion vessel 2 is tiled with a layer of glass balls 4, and the digestion vessel 2 is covered with the cover body 21 as shown in fig. 3. Then setting the working mode: 1. the operation is continued for several minutes. 2. Working for several minutes at intervals. The rotor traction disc 5 rotates, the spherical stirring rotors 3 are drawn to rotate by magnetic force, the glass balls 4 penetrate through gaps among the spherical stirring rotors 3, and cell tissues are digested into single cells by mutual friction.

Claims

1. A primary cell culture's soft tissue digestion device which characterized in that: it comprises a base, a digestion vessel, a spherical stirring rotor, glass balls and a rotor traction disc,

the rotor traction disc is positioned in the base and is driven to rotate by a motor; the rotor traction disc comprises blades which are arranged in a crossed manner, magnets are arranged on each blade at equal intervals, the distance between every two adjacent magnets is larger than the diameter of the glass ball, and the magnets on the two blades are arranged in a crossed manner; the magnets on the two blades are arranged in a crossed manner, and when the two blades are overlapped together, the magnets on the two blades are arranged in a staggered manner;

the spherical stirring rotor comprises an iron core and a ceramic layer wrapped outside the iron core, the thickness of the ceramic layer is 0.5-2mm, and the surface of the ceramic layer is a frosted surface; after the spherical stirring rotors are placed in the digestion vessel, the spherical stirring rotors are adsorbed by the corresponding magnets; a layer of glass balls is paved at the bottom of the digestion vessel;

a digestion vessel placing area is arranged on the upper surface of the base, and the blades are positioned below the digestion vessel placing area; the surface of the glass ball is a frosted surface;

a blade indicator of the rotor traction disc is arranged in the digestion vessel placing area, and position points of a spherical stirring rotor are drawn on the blade indicator; in the static state of a rotor traction disc, the magnet on the blade corresponds to the position point; the rotor traction disc rotates, the spherical stirring rotors are drawn by magnetic force to rotate, the glass balls pass through gaps among the spherical stirring rotors, and cell tissues are digested into single cells by mutual friction.

2. The primary cell culture soft tissue digestion apparatus of claim 1, wherein a controller is provided on the base that controls the motor, whereby the motor is operable to operate at timed and intermittent intervals.