CN206940908U - Antisitic defect NK cell culture apparatus - Google Patents
Antisitic defect NK cell culture apparatus Download PDFInfo
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- CN206940908U CN206940908U CN201720714368.3U CN201720714368U CN206940908U CN 206940908 U CN206940908 U CN 206940908U CN 201720714368 U CN201720714368 U CN 201720714368U CN 206940908 U CN206940908 U CN 206940908U
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- ware
- stepper motor
- bottom plate
- cell
- culture dish
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Abstract
The utility model discloses a kind of antisitic defect NK cell culture apparatus, including the culture dish being made up of ware body and ware lid；Also include rocking device, the device that rocks includes bottom plate, is arranged on the support bar of the side portion of bottom plate one, the ware set for laying culture dish and the first stepper motor for being arranged on the bearing of another side portion of bottom plate and being arranged on bearing, the ware, which is put, to be provided with and the upper end of support bar and the pivot being rotatably assorted, first stepper motor is located at ware set lower section, and the cam for promoting ware set to be moved around pivot center physical pendulum is provided with the rotating shaft of the first stepper motor.The utility model antisitic defect NK cell culture apparatus, ware body is placed in ware set, then rotated by the first stepper motor driving cam, cam rotation can promote culture dish to be rotated around pivot center, so that the culture medium and cell in culture dish are ceaselessly rocked, reach the effect of stirring, will not damage cell when can promote the merisis of cell, and stir.
Description
Technical field
Technical field of cell culture is the utility model is related to, more particularly to a kind of NK Tissue Culture Dish.
Background technology
NK (natural killer cell, NK cell) is the important immunocyte of body, not only with resisting
Tumour, viral infection resisting and immunological regulation are relevant, and participate in hypersensitivity and autoimmune disease in some cases
Occur.
With the development of biological therapy clinical practice, NK cells are more and more extensive in the research and application of medical field.Will
It is used for clinical treatment using NK cells, it is necessary first to expand substantial amounts of NK cells in vitro.
In NK cell cultivation process, it usually needs culture medium is stirred, to promote the flourish of NK cells.It is existing
Having in technology generally has mechanical agitation and ultrasonic wave to stir two kinds of forms of plate the agitating mode of cell culture medium.Existing machinery stirs
It is that agitating shaft is directly placed into culture medium to mix, and paddle can collide cell in whipping process, easily cause cellular damage；
And ultrasonic wave stirs, ultrasonic activation can produce a very large impact to cell, or even easily cause cell rupture；Therefore prior art
Middle generally use mechanical system is touched without paddle existing for solution mechanical agitation in the prior art to culture medium mechanical agitation
Hinder the technical problem of cell.
Utility model content
In view of this, the purpose of this utility model is to provide a kind of antisitic defect NK cell culture apparatus, to solve NK cells
In incubation, mechanical agitation is easily damaged the technical problem of cell.
The utility model antisitic defect NK cell culture apparatus, including the culture dish being made up of ware body and ware lid；Also include shaking
Dynamic device, the device that rocks include bottom plate, are arranged in the support bar of the side portion of bottom plate one, the ware for laying culture dish
Cover and be arranged on the bearing of another side portion of bottom plate and the first stepper motor being arranged on bearing, the ware, which is put, to be set
It is equipped with and is located at ware set lower section, and the first stepping electricity with the upper end of support bar and the pivot being rotatably assorted, first stepper motor
The cam for promoting ware set to be moved around pivot center physical pendulum is provided with the rotating shaft of machine.
Further, the support bar by sleeve pipe, be inserted in sleeve pipe and position can along sleeve pipe up and down adjust interior bar, be connected to
The U-shaped fork and the bolt for fixing interior bar of interior bar upper end supporting pivot form；The bearing by sleeve, be inserted in sleeve
And position can along sleeve up and down adjust pillar, be arranged on pillar upper end and be fixedly connected with the first stepper motor supporting plate,
And the bolt for fixing pillar forms；It is provided with the second stepper motor being vertically arranged in the middle part of the bottom plate, described
The rotating disk being fixedly connected in the rotating shaft of two stepping motor, positioning hole is provided with the rotating disk, the bottom of the ware body is provided with
For the pin coordinated with positioning hole.
The beneficial effects of the utility model：
1st, the utility model antisitic defect NK cell culture apparatus, ware body is placed in ware set, then passes through the first stepping electricity
Machine driving cam rotates, and cam rotation can promote culture dish to be rotated around pivot center so that culture medium in culture dish and
Cell is ceaselessly rocked, and reaches the effect of stirring, and will not damage cell when can promote the merisis of cell, and stir.
2nd, the utility model antisitic defect NK cell culture apparatus, it can also turn down the height of support bar and bearing, make ware
Set separates with support bar and bearing, and makes in the locating piece of pin insertion rotating disk of ware set bottom, realizes the connection of ware set and rotating disk,
So as to be rotated by the second driving stepper motor culture dish, the function of rotary stand of culture dish can be played so that can be in culture dish
In be inoculated with, be coated with, the operation such as making sheet.
Brief description of the drawings
Fig. 1 is that the sectional structure of the utility model antisitic defect NK cell culture apparatus is illustrated.
Embodiment
The utility model is further described with reference to the accompanying drawings and examples.
As illustrated, the present embodiment antisitic defect NK cell culture apparatus, including the culture dish being made up of ware body 1 and ware lid 2；
Also include rocking device, the device that rocks includes bottom plate 3, is arranged in the support bar 4 of the side portion of bottom plate one, for laying and trains
The ware set 5 of ware and the first stepper motor 7 for being arranged on the bearing 6 of another side portion of bottom plate and being arranged on bearing are supported,
The ware puts to be provided with is located at ware set lower section with the upper end of support bar and the pivot 8 being rotatably assorted, first stepper motor,
And first stepper motor rotating shaft on be provided with promote ware set around pivot center physical pendulum move cam 9.
The present embodiment antisitic defect NK cell culture apparatus, ware body is placed in ware set, then driven by the first stepper motor
Moving cam rotates, and cam rotation can promote culture dish to be rotated around pivot center, so that culture medium and cell in culture dish
Ceaselessly rocked, reach the effect of stirring, will not damage cell when can promote the merisis of cell, and stir.
In the present embodiment, the support bar 4 by sleeve pipe 401, be inserted in sleeve pipe and position can along sleeve pipe up and down adjust along
Bar 402, the U-shaped fork 403 for being connected to interior bar upper end supporting pivot and the bolt 404 for fixing interior bar form；The bearing
6 by sleeve 601, be inserted in sleeve and position can along sleeve up and down adjust pillar 602, be arranged on pillar upper end and and the first step
The supporting plate 603 and the bolt 604 for fixing pillar that stepper motor is fixedly connected form；It is provided with the middle part of the bottom plate
The second stepper motor 10 being vertically arranged, the rotating disk 11 being fixedly connected in the rotating shaft of second stepper motor, on the rotating disk
Positioning hole 12 is provided with, the bottom of the ware body is provided with the pin 13 for coordinating with positioning hole.
This improvement makes it possible to turn down the height of support bar and bearing, ware set is separated with support bar and bearing, and make ware
In the locating piece of pin insertion rotating disk for covering bottom, the connection of ware set and rotating disk is realized, so as to pass through the second driving stepper motor
Culture dish rotates, and can play the function of rotary stand of culture dish so that can be inoculated with, is coated with culture dish, the operation such as making sheet.
Finally illustrate, above example is only unrestricted to illustrate the technical solution of the utility model, although ginseng
The utility model is described in detail according to preferred embodiment, it will be understood by those within the art that, can be to this
The technical scheme of utility model is modified or equivalent substitution, without departing from the objective and model of technical solutions of the utility model
Enclose, it all should cover among right of the present utility model.
Claims (2)
1. a kind of antisitic defect NK cell culture apparatus, including the culture dish being made up of ware body and ware lid；It is characterized in that：Also include
Device is rocked, the device that rocks includes bottom plate, is arranged on the support bar of the side portion of bottom plate one, the ware for laying culture dish
Cover and be arranged on the bearing of another side portion of bottom plate and the first stepper motor being arranged on bearing, the ware, which is put, to be set
It is equipped with and is located at ware set lower section, and the first stepping electricity with the upper end of support bar and the pivot being rotatably assorted, first stepper motor
The cam for promoting ware set to be moved around pivot center physical pendulum is provided with the rotating shaft of machine.
2. antisitic defect NK cell culture apparatus according to claim 1, it is characterised in that：The support bar by sleeve pipe, insert
In sleeve pipe and interior bar that position can be adjusted along above and below sleeve pipe, it is connected to the U-shaped fork of interior bar upper end supporting pivot and for solid
Determine the bolt composition of interior bar；The bearing by sleeve, be inserted in sleeve and position can along sleeve up and down adjust pillar, be arranged on
Pillar upper end and the supporting plate that is fixedly connected with the first stepper motor and the bolt for fixing pillar form；The bottom plate
Middle part be provided with the second stepper motor being vertically arranged, the rotating disk being fixedly connected in the rotating shaft of second stepper motor, institute
State and positioning hole is provided with rotating disk, the bottom of the ware body is provided with the pin for coordinating with positioning hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720714368.3U CN206940908U (en) | 2017-06-19 | 2017-06-19 | Antisitic defect NK cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720714368.3U CN206940908U (en) | 2017-06-19 | 2017-06-19 | Antisitic defect NK cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206940908U true CN206940908U (en) | 2018-01-30 |
Family
ID=61364416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720714368.3U Active CN206940908U (en) | 2017-06-19 | 2017-06-19 | Antisitic defect NK cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206940908U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112481082A (en) * | 2020-12-18 | 2021-03-12 | 韩雪梅 | Anti-pollution formula bacteria culture device of clinical laboratory |
-
2017
- 2017-06-19 CN CN201720714368.3U patent/CN206940908U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112481082A (en) * | 2020-12-18 | 2021-03-12 | 韩雪梅 | Anti-pollution formula bacteria culture device of clinical laboratory |
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