CN101650274A - High pass tissue grinder and grinding method - Google Patents
High pass tissue grinder and grinding method Download PDFInfo
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- CN101650274A CN101650274A CN200910101884A CN200910101884A CN101650274A CN 101650274 A CN101650274 A CN 101650274A CN 200910101884 A CN200910101884 A CN 200910101884A CN 200910101884 A CN200910101884 A CN 200910101884A CN 101650274 A CN101650274 A CN 101650274A
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Abstract
The invention discloses a high pass tissue grinder and a grinding method. 1-5 grinding balls are placed into a sample pipe, and the diameters of the grinding balls are between 2 mm and 30 mm; a samplebox is immerged into liquid nitrogen to be cooled for 1 minute-2 minutes; the sample box reciprocates under the action of a swing mechanism; and the reciprocating times of the swing mechanism is 200times/minute-3000 times/minute. The invention has the advantages that the grinding balls are placed into the sample pipe, the sample pipe is vibrated in a reciprocating way along a vertical directionunder the action of the swing mechanism or along a horizontal direction or along an arc direction, the grinding balls impacts samples at high speed to complete the grinding of the samples, the frictional impact among the grinding balls can effectively lead to the smash of sample tissues or cells; the closed sample pipe is used so as to be difficult to cause cross infection among raw material samples; and in addition, multiple samples can be simultaneously fast and effectively ground within 2-4 minutes, thus the grinding efficiency is greatly enhanced.
Description
Technical field
The present invention relates to a kind of Ginding process, especially relate to a kind of high-flux tissue grinder and Ginding process.
Background technology
High-flux tissue grinder can be used for to multiple different materials dry grind, milling and mix and the processing that homogenizes under wet-milling or the cryogenic conditions.The kind of material comprises plant tissue, animal tissue, cell, bacterium and yeast or the like.Nucleic acid for extracting in the big flux sample at first needs sample is ground fully, makes the tissue or the clasmatosis of sample.The high flux muller not only can help vast scientific research user to conform to the principle of simplicity to free in the multiple manual labor of substance, reduces workload widely, can improve grinding efficiency and quality simultaneously.But the most complex structure of present muller, and the effect of grinding raw material sample is bad.
Summary of the invention
Technical matters to be solved by this invention provides a kind of high-flux tissue grinder simple in structure, and the good Ginding process of material sample grinding effect.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of high-flux tissue grinder, it is characterized in that it includes the support and the tilting mechanism of motor, placement sample box, described motor is connected with described support by tilting mechanism, described support is provided with the stationary installation that sample box can be fixed, but described sample box is provided with the cover plate of fixed sample pipe.
Described sample box comprises upper mounted plate and bottom plate, be provided with support column between described upper mounted plate and the described bottom plate, described upper mounted plate is provided with a plurality of first pores, and described bottom plate is provided with and corresponding second pore of described first pore.
Described tilting mechanism comprises eccentric wheel and driving shaft, and described eccentric wheel is arranged on the output shaft of motor, and eccentric wheel is connected with described support by driving shaft.
Described stationary installation comprises the bolt that passes support, is set with quick set nut on the described bolt, and described bolt can withstand the sample box that is placed in the support.
The quantity of described first pore and second pore is respectively 96.
A kind of Ginding process of high-flux tissue grinder is characterized in that it may further comprise the steps:
(1), material sample is circulated in the sample hose; (2), put into 1～5 mill ball in the sample hose, the diameter of mill ball is between 2mm～30mm, and the sample hose caliber seals sample hose greater than mill ball diameter 0.01mm～3mm again with lid; (3), all sample hoses that pour into material sample are inserted into respectively in first pore and second pore of sample box, with cover plate sample hose is fixed in the sample box then; (4), sample box is immersed in the liquid nitrogen cooling 1 minute～5 minutes; (5), have the sample box of a plurality of sample hoses to put into the support of muller storing, with stationary installation sample box is fixed in the support; (6), start muller, the tilting mechanism action in the muller, sample box to-and-fro movement under the effect of tilting mechanism; The tilting mechanism reciprocal time is 200 times/per minute～3000 time/per minute, grinds built-in temperature and is controlled at certain certain value in 20 degree～40 degree scopes.
Described mill ball is steel mill ball or ceramic grinding ball.
Sample box is made vertical direction double vibrations or horizontal direction double vibrations or is made camber line shape double vibrations.
Compared with prior art, advantage of the present invention is the rotation of motor, and the tilting mechanism driving arm is moved reciprocatingly, and the sample box in the support moves with support, muller simple in structure.When sample hose was seated in the sample box, sample hose was inserted in first pore and second pore.Motor makes the driving shaft movement in vertical direction by eccentric wheel, thereby makes the sample hose movement in vertical direction in the sample box.Set nut can make the sample box in the quick fixed support of bolt fast.
The advantage of this Ginding process is for putting into mill ball in sample hose, sample hose is made vertical direction double vibrations or horizontal direction double vibrations or camber line shape double vibrations under the effect of tilting mechanism, under high-frequency vibration, mill ball impacts sample at a high speed and finishes grinding to sample, and the friction bump between ball and the ball can effectively cause the fragmentation of sample tissue or cell.Use the sample hose of closure, be difficult for causing the cross-infection between material sample.In 2～4 minutes, can grind fast and effectively simultaneously a plurality of samples, improved grinding efficiency widely.
Description of drawings
Fig. 1 is for putting into the synoptic diagram of mill ball in the sample hose of the present invention;
Fig. 2 is a sample box structural drawing of the present invention;
Fig. 3 is grinding machine structure figure of the present invention.
Embodiment
Embodiment describes in further detail the present invention below in conjunction with accompanying drawing.
Embodiment one: a kind of high-flux tissue grinder, it includes motor 1, places the support 3 and the tilting mechanism of sample box 2, motor 1 is connected with support 3 by tilting mechanism, support 3 is provided with the stationary installation that sample box 2 can be fixed, but sample box 2 is provided with the cover plate 5 of fixed sample pipe 4.Sample box 2 comprises upper mounted plate 6 and bottom plate 7, is provided with support column 8 between upper mounted plate 6 and the bottom plate 7, and upper mounted plate 6 is provided with a plurality of first pores 9, and 7 are provided with and first pore, 9 corresponding second pores 10 on the bottom plate.Tilting mechanism comprises eccentric wheel 11 and driving shaft 12, and eccentric wheel 11 is arranged on the output shaft of motor 1, and eccentric wheel 11 is connected with support by driving shaft 12.
Stationary installation comprises the bolt 13 that passes support 3, is set with quick set nut 14 on the bolt 13, and bolt 13 can withstand the sample box 2 that is placed in the support 3.The quantity of first pore 9 and second pore 10 is respectively 96.
A kind of Ginding process of high-flux tissue grinder is characterized in that it may further comprise the steps:
(1), material sample 15 is circulated in the sample hose 4; (2), put into 2 steel mill balls 16 in the sample hose 4, the diameter of steel mill ball 16 is 5mm, and sample hose 4 calibers are greater than steel mill ball 16 diameter 0.2mm, again with 17 sealings of sample hose 4 usefulness lids; (3), all sample hoses 4 that pour into material sample 15 are inserted into respectively in first pore 9 and second pore 10 of sample box 2, with cover plate 5 sample hose 4 is fixed in the sample box 2 then; (4), with cooling in the sample box 2 immersion liquid nitrogen 1 minute 15 seconds; (5), have the sample box 2 of a plurality of sample hoses 4 to put into the support 3 of muller storing, with stationary installation sample box 2 is fixed in the support 3; (6), start muller, the tilting mechanism action in the muller, sample box 2 is made the vertical direction double vibrations under the effect of tilting mechanism; The tilting mechanism reciprocal time is 300 a times/per minute, and thermostatic control is at 25 degree in the muller.
Embodiment two: a kind of Ginding process of high-flux tissue grinder is characterized in that it may further comprise the steps: (1), material sample 15 is circulated in the sample hose 4; (2), put into 3 ceramic grinding balls 16 in the sample hose 4, the diameter of ceramic grinding ball 16 is between the 10mm, and sample hose 4 calibers are greater than ceramic grinding ball 16 diameter 1mm, again with 17 sealings of sample hose 4 usefulness lids; (3), all sample hoses 4 that pour into material sample 15 are inserted into respectively in first pore 9 and second pore 10 of sample box 2, with cover plate 5 sample hose 4 is fixed in the sample box 2 then; (4), with cooling in the sample box 2 immersion liquid nitrogen 3 minutes; (5), have the sample box 2 of a plurality of sample hoses 4 to put into the support 3 of muller storing, with stationary installation sample box 2 is fixed in the support 3; (6), start muller, the tilting mechanism action in the muller, sample box 2 is made the horizontal direction double vibrations under the effect of tilting mechanism; The tilting mechanism reciprocal time is 1000 a times/per minute, and thermostatic control is at 30 degree in the muller.
Embodiment three: a kind of Ginding process of high-flux tissue grinder is characterized in that it may further comprise the steps: (1), material sample 15 is circulated in the sample hose 4; (2), put into 5 ceramic grinding balls 16 in the sample hose 4, the diameter of ceramic grinding ball 16 is between the 2-10mm, and sample hose 4 calibers are greater than ceramic grinding ball 16 diameter 2.5mm, again with 17 sealings of sample hose 4 usefulness lids; (3), all sample hoses 4 that pour into material sample 15 are inserted into respectively in first pore 9 and second pore 10 of sample box 2, with cover plate 5 sample hose 4 is fixed in the sample box 2 then; (4), with cooling in the sample box 2 immersion liquid nitrogen 4 minutes; (5), have the sample box 2 of a plurality of sample hoses 4 to put into the support 3 of muller storing, with stationary installation sample box 2 is fixed in the support 3; (6), start muller, the tilting mechanism action in the muller, sample box 2 is made camber line shape double vibrations under the effect of tilting mechanism; The tilting mechanism reciprocal time is 2500 a times/per minute, and thermostatic control is at 35 degree in the muller.
Claims (8)
1. high-flux tissue grinder, it is characterized in that it includes the support and the tilting mechanism of motor, placement sample box, described motor is connected with described support by tilting mechanism, described support is provided with the stationary installation that sample box can be fixed, but described sample box is provided with the cover plate of fixed sample pipe.
2. a kind of high-flux tissue grinder according to claim 1, it is characterized in that described sample box comprises upper mounted plate and bottom plate, be provided with support column between described upper mounted plate and the described bottom plate, described upper mounted plate is provided with a plurality of first pores, and described bottom plate is provided with and corresponding second pore of described first pore.
3. a kind of high-flux tissue grinder according to claim 1 is characterized in that described tilting mechanism comprises eccentric wheel and driving shaft, and described eccentric wheel is arranged on the output shaft of motor, and eccentric wheel is connected with described support by driving shaft.
4. a kind of high-flux tissue grinder according to claim 1 is characterized in that described stationary installation comprises the bolt that passes support, is set with quick set nut on the described bolt, and described bolt can withstand the sample box that is placed in the support.
5. a kind of high-flux tissue grinder according to claim 2 is characterized in that the quantity of described first pore and second pore is respectively 96.
6. the Ginding process of a kind of high-flux tissue grinder according to claim 1 is characterized in that it may further comprise the steps:
(1), material sample is circulated in the sample hose; (2), put into 1～5 mill ball in the sample hose, the diameter of mill ball is between 2mm～30mm, and the sample hose caliber seals sample hose greater than mill ball diameter 0.01mm～3mm again with lid; (3), all sample hoses that pour into material sample are inserted into respectively in first pore and second pore of sample box, with cover plate sample hose is fixed in the sample box then; (4), sample box is immersed in the liquid nitrogen cooling 1 minute～5 minutes; (5), have the sample box of a plurality of sample hoses to put into the support of muller storing, with stationary installation sample box is fixed in the support; (6), start muller, the tilting mechanism action in the muller, sample box to-and-fro movement under the effect of tilting mechanism; The tilting mechanism reciprocal time is 200 times/per minute～3000 time/per minute, grinds built-in temperature and is controlled at certain certain value in 20 degree～40 degree scopes.
7. the Ginding process of a kind of high-flux tissue grinder according to claim 6 is characterized in that described mill ball is steel mill ball or ceramic grinding ball.
8. the Ginding process of a kind of high flux muller according to claim 6 is characterized in that sample box makes vertical direction double vibrations or horizontal direction double vibrations or make camber line shape double vibrations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910101884A CN101650274A (en) | 2009-08-31 | 2009-08-31 | High pass tissue grinder and grinding method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910101884A CN101650274A (en) | 2009-08-31 | 2009-08-31 | High pass tissue grinder and grinding method |
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| CN101650274A true CN101650274A (en) | 2010-02-17 |
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| CN200910101884A Pending CN101650274A (en) | 2009-08-31 | 2009-08-31 | High pass tissue grinder and grinding method |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101852695A (en) * | 2010-04-30 | 2010-10-06 | 宁波新芝生物科技股份有限公司 | High-flux tissue grinder |
| CN102288462A (en) * | 2011-01-25 | 2011-12-21 | 向华 | High-throughput tissue grinder |
| CN102814214A (en) * | 2012-08-20 | 2012-12-12 | 广西壮族自治区药用植物园 | Huperzia serrate spore wall breaking method |
| CN102824943A (en) * | 2012-08-23 | 2012-12-19 | 中国人民解放军军事医学科学院卫生装备研究所 | Universal cell rapid breaker using bead vibrating and grinding |
| CN102896033A (en) * | 2012-08-22 | 2013-01-30 | 王丽华 | Application method of high-flux plant leaf crusher |
| CN103018072A (en) * | 2012-11-30 | 2013-04-03 | 安徽科技学院 | Application method of cluster type plane leaf collecting and crushing device |
| CN104596819A (en) * | 2015-01-19 | 2015-05-06 | 中国人民解放军第四军医大学 | Grinding device for biological tissue |
| CN105754987A (en) * | 2014-12-14 | 2016-07-13 | 仲恺农业工程学院 | Method for rapidly crushing plants in batches to extract biomacromolecules from leaves |
| CN106885722A (en) * | 2017-02-17 | 2017-06-23 | 中国农业科学院棉花研究所 | A kind of crops dry grind away method |
| CN107058085A (en) * | 2017-03-09 | 2017-08-18 | 武汉赛维尔生物科技有限公司 | A kind of full-automatic tissue grinder instrument of high flux |
| CN108753611A (en) * | 2018-06-06 | 2018-11-06 | 青岛农业大学 | The soft tissue slaking apparatus of primitive cell culture |
| US10537897B2 (en) | 2016-09-30 | 2020-01-21 | Industrial Technology Research Institute | Grinding machine and a slight gyration module |
-
2009
- 2009-08-31 CN CN200910101884A patent/CN101650274A/en active Pending
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101852695A (en) * | 2010-04-30 | 2010-10-06 | 宁波新芝生物科技股份有限公司 | High-flux tissue grinder |
| CN101852695B (en) * | 2010-04-30 | 2011-11-30 | 宁波新芝生物科技股份有限公司 | High-flux tissue grinder |
| CN102288462A (en) * | 2011-01-25 | 2011-12-21 | 向华 | High-throughput tissue grinder |
| CN102288462B (en) * | 2011-01-25 | 2012-07-18 | 北京托摩根生物科技有限公司 | High-throughput tissue grinder |
| CN102814214B (en) * | 2012-08-20 | 2014-11-12 | 广西壮族自治区药用植物园 | Huperzia serrate spore wall breaking method |
| CN102814214A (en) * | 2012-08-20 | 2012-12-12 | 广西壮族自治区药用植物园 | Huperzia serrate spore wall breaking method |
| CN102896033A (en) * | 2012-08-22 | 2013-01-30 | 王丽华 | Application method of high-flux plant leaf crusher |
| CN102824943A (en) * | 2012-08-23 | 2012-12-19 | 中国人民解放军军事医学科学院卫生装备研究所 | Universal cell rapid breaker using bead vibrating and grinding |
| CN102824943B (en) * | 2012-08-23 | 2014-12-10 | 中国人民解放军军事医学科学院卫生装备研究所 | Universal cell rapid breaker using bead vibrating and grinding |
| CN103018072A (en) * | 2012-11-30 | 2013-04-03 | 安徽科技学院 | Application method of cluster type plane leaf collecting and crushing device |
| CN105754987A (en) * | 2014-12-14 | 2016-07-13 | 仲恺农业工程学院 | Method for rapidly crushing plants in batches to extract biomacromolecules from leaves |
| CN104596819A (en) * | 2015-01-19 | 2015-05-06 | 中国人民解放军第四军医大学 | Grinding device for biological tissue |
| CN104596819B (en) * | 2015-01-19 | 2017-04-05 | 中国人民解放军第四军医大学 | A kind of lapping device for biological tissue |
| US10537897B2 (en) | 2016-09-30 | 2020-01-21 | Industrial Technology Research Institute | Grinding machine and a slight gyration module |
| CN106885722A (en) * | 2017-02-17 | 2017-06-23 | 中国农业科学院棉花研究所 | A kind of crops dry grind away method |
| CN107058085A (en) * | 2017-03-09 | 2017-08-18 | 武汉赛维尔生物科技有限公司 | A kind of full-automatic tissue grinder instrument of high flux |
| CN108753611A (en) * | 2018-06-06 | 2018-11-06 | 青岛农业大学 | The soft tissue slaking apparatus of primitive cell culture |
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Application publication date: 20100217 |