CN102896033A - Application method of high-flux plant leaf crusher - Google Patents
Application method of high-flux plant leaf crusher Download PDFInfo
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- CN102896033A CN102896033A CN2012103000875A CN201210300087A CN102896033A CN 102896033 A CN102896033 A CN 102896033A CN 2012103000875 A CN2012103000875 A CN 2012103000875A CN 201210300087 A CN201210300087 A CN 201210300087A CN 102896033 A CN102896033 A CN 102896033A
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- grinder
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- centrifuge tube
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Abstract
A high-flux plant leaf crusher comprises a plastic centrifugal pipe and a steel ball, wherein the steel ball is positioned at the bottom of the crusher. When in use, plant leaves are cut into pieces; the pieces are placed into crushers; the crushers are covered; a plurality of crushers containing samples are added into a foamed plastic box; liquid nitrogen is injected into the box; the foamed plastic box is covered, and are subjected to standing for approximately 1min; the crushers are fetched and are placed in a centrifugal pipe box; and the centrifugal pipe box is covered and is violently oscillated for 10 seconds by using hand, such that the leave samples are completely crushed. The crusher provided by the invention has the advantages of simple operation, high speed, high efficiency, and no cross contamination among the samples.
Description
Affiliated field
The invention belongs to a kind of method of research of agricultural science, specifically a kind of using method of high flux plant leaf blade grinder.
Background technology
In the process of plant extract DNA and protein, the first step will be pulverized plant leaf blade exactly, existing grind away method is to add sample and quartz sand (or liquid nitrogen) in mortar, grind with grinding rod, grind a sample and need about 1 minute, then will shift sample, clean mortar, wiped clean, and then grind next sample.The way that this mortar grinds wastes time and energy, and the sample number is when more, because mortar cleans the problem of the mutual cross pollution of sample room that totally also can not produce.So, create a kind of convenient and strength-saving, quickness and high efficiency and can not cause again the blade grinder of sample room cross pollution and breaking method very necessary.
Summary of the invention
The present invention is a kind of using method of high-throughout plant leaf blade grinder, and it is simple to have use equipment, and processing ease, sample room mutually can cross pollutions, and sample is pulverized in batches, the advantage of quickness and high efficiency.
The structure of product of the present invention:
A kind of high flux plant leaf blade grinder is made of a plastic centrifuge tube 1 and a stainless steel ball 2.The size of centrifuge tube and steel ball is decided as required.Stainless steel ball is placed on centrifuge tube bottom (Fig. 1).
Product of the present invention is to use like this:
1. blade is removed large vein, be cut into small pieces and put into grinder.
2. the blade amount of putting into is no more than 1/4 of grinder volume.
3. build the lid of grinder.
4. a plurality of grinders are put into a foam plastic magazine, injected liquid nitrogen in box, injection rate must be flooded all grinders.
5. build the foam lid, left standstill about 1 minute, make blade quick-frozen under the ultralow temperature of liquid nitrogen become crisp thin slice.
6. with strainer grinder is pulled out, got 36 ends that install to 72 hole centrifuge tube boxes.
7. build the centrifuge tube lid, two hold the other end of centrifuge tube box, firmly shake up and down about 10 seconds fast take elbow as fulcrum.Blade in the grinder is pulverized totally under the strong repeatedly bump of steel ball in a moment.
8. open the lid, take out rapidly grinder, open the lid of grinder, pour out steel ball.(annotate: speed must be fast, can pour out otherwise pulverize sample thereupon.）
9. the leaf sample of pulverizing is transferred to new container.
10. the later operation of step 4 will be worn gloves and be prevented frostbite skin on hand.
Leaf sample is pulverized complete.
The plant leaf blade sample that adopts method of the present invention to pulverize meets the requirement of subsequent chemical analysis fully.
Characteristics of the present invention:
Breaking method of the present invention has been compared following characteristics with existing mortar polishing;
1. will grind and change freezing crashing into.It is to be ground to the master with mortar that existing plant leaf blade is pulverized.Sample of the every grinding of this method approximately needs 1 minutes, then also will shift sample, cleans mortar, 2-3 minute again.Use the present invention packs leaf sample into after the grinder, together with grinder freezing through about one minute under-196 ℃ the ultralow temperature in liquid nitrogen, so that leaf sample becomes loose crisp thin slice, then in a moment broken at the strong repeatedly bump Leaf of steel ball is fine powder.Pulverizing speed is fast, and efficient is high.
2. become the single batch processing that is operating as one by one.It is staff one alms bowl that mortar grinds blade, grind singly, and grinder of the present invention can batch process, once pulverizes 36 samples.In conjunction with the advantage of characteristics 1, estimate that operating efficiency can improve 100 times.
3. it is convenient to shift sample.Because the tube wall in the grinder is smooth plastic tube wall, when shifting, sample first sample is toppled over out, then use distilled water wash bottle one flushing thoroughly.
4. if plant leaf blade is used for the extraction of DNA, need not shift sample, pour out steel ball after, directly just in centrifuge tube, continue further work.More convenient.
Description of drawings
Fig. 1 is high flux plant leaf blade grinder profilograph.
Among the figure: 1 centrifuge tube; 2 steel balls.
The specific embodiment
The fast-crushing of embodiment 1. Sorghum Leaves:
1. Sorghum Leaves except being cut into small pieces, the large arteries and veins of defoliation is put into grinder.
2. the blade amount of putting into is no more than 1/4 of grinder volume.
3. build the grinder lid.
4. a plurality of grinders are put into a foam plastic magazine, injected liquid nitrogen in box, injection rate must be flooded whole grinders.
5. build the foam lid, left standstill 1 minute.
6. with strainer grinder is pulled out, got 36 ends that install to the centrifuge tube box in 72 holes.
7. build the centrifuge tube lid, two hold the other end of centrifuge tube box, firmly shake up and down for 10 seconds fast take elbow as fulcrum.Blade in the grinder is pulverized totally under the strong repeatedly bump of steel ball.
8. open the lid, take out rapidly grinder, open the lid of grinder, pour out steel ball.
Sorghum Leaves is pulverized complete.
The fast-crushing of embodiment 2. maize leafs:
1. maize leaf being removed large vein is cut into small pieces and puts into grinder.
2. the blade amount of putting into is no more than 1/4 of grinder volume.
3. build the lid of grinder.
4. a plurality of grinders are put into a foam plastic magazine, injected liquid nitrogen in box, injection rate must be flooded whole grinders.
5. build the foam lid, left standstill 1 minute.
6. with strainer grinder is pulled out, got 36 ends that install to the centrifuge tube box in 72 holes.
7. build the centrifuge tube lid, two hold the other end of centrifuge tube box, firmly shake up and down for 10 seconds fast take elbow as fulcrum.Blade in the grinder is pulverized totally under the strong repeatedly bump of steel ball.
8. open the lid, take out rapidly grinder, open the grinder lid and pour out steel ball.
The maize leaf blade is pulverized complete.
Claims (5)
- A kind of using method of high flux plant leaf blade grinder by the dress sample, freezing, pulverize, shift four steps of sample and form, it is characterized in that crushing process does not need to grind one by one, as long as with about 10 seconds of lower concussion on hand.
- The dress sampleBlade is removed large vein, be cut into small pieces and put into grinder;The blade amount of putting into is no more than 1/4 of grinder volume;Build the lid of grinder.
- FreezingA plurality of grinders are put into a foam plastic magazine, inject liquid nitrogen in box, injection rate must be flooded all grinders;Build the foam lid, left standstill about 1 minute.
- Pulverize1. with strainer grinder is pulled out, got 36 ends that install to 72 hole centrifuge tube boxes;Build the centrifuge tube lid, two hold the other end of centrifuge tube box, firmly shake up and down about 10 seconds take elbow as fulcrum.
- ShiftOpen the lid, take out rapidly grinder, open the lid of grinder, pour out steel ball;Sample in the grinder is changed in the new container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103000875A CN102896033A (en) | 2012-08-22 | 2012-08-22 | Application method of high-flux plant leaf crusher |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103000875A CN102896033A (en) | 2012-08-22 | 2012-08-22 | Application method of high-flux plant leaf crusher |
Publications (1)
| Publication Number | Publication Date |
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| CN102896033A true CN102896033A (en) | 2013-01-30 |
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| CN2012103000875A Pending CN102896033A (en) | 2012-08-22 | 2012-08-22 | Application method of high-flux plant leaf crusher |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105018473A (en) * | 2015-09-02 | 2015-11-04 | 中国科学院新疆生态与地理研究所 | Method for crushing samples in batches for plant RNA extraction |
| CN105754987A (en) * | 2014-12-14 | 2016-07-13 | 仲恺农业工程学院 | Method for rapidly grinding plant leaves in batch and extracting biomacromolecules |
| CN105758692A (en) * | 2016-04-29 | 2016-07-13 | 中国科学院西北高原生物研究所 | Macro fungus pulverizing method suitable for laboratory use |
| CN106885722A (en) * | 2017-02-17 | 2017-06-23 | 中国农业科学院棉花研究所 | A kind of crops dry grind away method |
| CN108593496A (en) * | 2018-04-25 | 2018-09-28 | 江汉大学 | A kind of measurement method of crops cadmium ion content |
| CN110465381A (en) * | 2019-07-02 | 2019-11-19 | 苏州市吴中区固体废弃物处理有限公司 | A kind of solid waste Cold pretreatment device and technique |
Citations (4)
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| US7344675B2 (en) * | 2003-03-12 | 2008-03-18 | The Boeing Company | Method for preparing nanostructured metal alloys having increased nitride content |
| CN101650274A (en) * | 2009-08-31 | 2010-02-17 | 宁波新芝生物科技股份有限公司 | High pass tissue grinder and grinding method |
| CN102159931A (en) * | 2008-09-18 | 2011-08-17 | 恰根有限公司 | Method and apparatus for the simultaneous, automated decomposition of a plurality of biological samples |
| CN102288462A (en) * | 2011-01-25 | 2011-12-21 | 向华 | High-throughput tissue grinder |
-
2012
- 2012-08-22 CN CN2012103000875A patent/CN102896033A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7344675B2 (en) * | 2003-03-12 | 2008-03-18 | The Boeing Company | Method for preparing nanostructured metal alloys having increased nitride content |
| CN102159931A (en) * | 2008-09-18 | 2011-08-17 | 恰根有限公司 | Method and apparatus for the simultaneous, automated decomposition of a plurality of biological samples |
| CN101650274A (en) * | 2009-08-31 | 2010-02-17 | 宁波新芝生物科技股份有限公司 | High pass tissue grinder and grinding method |
| CN102288462A (en) * | 2011-01-25 | 2011-12-21 | 向华 | High-throughput tissue grinder |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754987A (en) * | 2014-12-14 | 2016-07-13 | 仲恺农业工程学院 | Method for rapidly grinding plant leaves in batch and extracting biomacromolecules |
| CN105018473A (en) * | 2015-09-02 | 2015-11-04 | 中国科学院新疆生态与地理研究所 | Method for crushing samples in batches for plant RNA extraction |
| CN105758692A (en) * | 2016-04-29 | 2016-07-13 | 中国科学院西北高原生物研究所 | Macro fungus pulverizing method suitable for laboratory use |
| CN106885722A (en) * | 2017-02-17 | 2017-06-23 | 中国农业科学院棉花研究所 | A kind of crops dry grind away method |
| CN108593496A (en) * | 2018-04-25 | 2018-09-28 | 江汉大学 | A kind of measurement method of crops cadmium ion content |
| CN110465381A (en) * | 2019-07-02 | 2019-11-19 | 苏州市吴中区固体废弃物处理有限公司 | A kind of solid waste Cold pretreatment device and technique |
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Application publication date: 20130130 |