CN102288462A - High-throughput tissue grinder - Google Patents
High-throughput tissue grinder Download PDFInfo
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- CN102288462A CN102288462A CN 201110168356 CN201110168356A CN102288462A CN 102288462 A CN102288462 A CN 102288462A CN 201110168356 CN201110168356 CN 201110168356 CN 201110168356 A CN201110168356 A CN 201110168356A CN 102288462 A CN102288462 A CN 102288462A
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Abstract
The invention relates to a high-throughput tissue grinder, which comprises a base, a driving component, an oscillation component and a clamp component, wherein the driving component and the oscillation component are arranged on the base; the clamp component is driven by the oscillation component to oscillate and used for clamping a sample; the oscillation component comprises a guide rod which is fixed on the base and a bracket arm which is arranged on the guide rod in a vertical sliding mode and driven by the driving component; a vertical sliding rail which is matched with the guide rod is arranged on the side face of the bracket arm; and the clamp component is fixed on the bracket arm. By arranging the oscillation component which comprises the guide rod and the bracket arm and matching the guide rod and the vertical sliding rail which is arranged on the bracket arm, the clamp component which is positioned on the bracket arm vibrates in the vertical direction, higher-frequency oscillation can be born, and processing efficiency is greatly improved; moreover, because resonance is difficult to produce, the noise of the working process of the high-throughput tissue grinder is lower.
Description
[technical field]
The present invention relates to a kind of bio-science research instrument, especially relate to the broken fast high-flux tissue beveller of a kind of animal vegetable tissue cell.
[background technology]
Cell breaking technology is meant and utilizes outside destroy cell membrane and cell membrane, makes cellular content comprise the technology that the purpose product component discharges, and is the basis of the interior nonsecreting type biochemical substances (product) that synthesizes of separation and purification cell.Developed the various kinds of cell breaking method at present, so that adapt to different purposes and dissimilar cell membrane fragmentations.Breaking method can be advised to receive and is Mechanical Method and on-mechanical method two big classes.Mechanical Method comprises the high-pressure homogenization crush method, the Tang Zhu that shakes hits crush method, the high-speed stirred pearl is ground crush method, ultrasonic disruption method; The on-mechanical method comprises osmotic pressure impact grinding method, freeze thawing crush method, the molten crush method of enzyme, chemically fragmenting method, scaling agent crush method.
The general broken time of on-mechanical method is long, is not suitable for the fast detecting aspect of sample.The Mechanical Method general operation is more violent, and wherein high-pressure homogenization crush method and high-speed stirred pearl are ground crush method and be mainly used in industrial extraction to various materials.The ultrasonic disruption method is because the restriction of equipment is applied to industrial fewer.It is that isopyknic a small amount of tissue samples and highdensity mill ball (ZircoBeads) are put into sealable 2ml screw top micro tube that the Tang Zhu that shakes hits crush method, adds damping fluid and stable elements to 1 again.The volume of 5ml was organized the beveller vibration at high speed 8 seconds with 6500rpm, had a rest 8 seconds, vibrated 8 seconds again and got final product.The method is at present the fastest and once can handles the method for maximum samples---a machine can be handled 2400 samples at one day at most, this concerning handle in a small amount and a plurality of sample easily, but then can't satisfy the demand when big for treatment capacity.
The high-flux tissue beveller that the Tang Zhu that shakes hits crush method mainly is at the adding in vitro that sample the is housed Tang Zhu that shakes, the test tube group carried out putting into after liquid nitrogen is handled carry out the level vibration on the instrument, Tang Zhuneng breaks up cell tissue in vitro so that shake, and reaches the purpose of extracting its inner nucleic acid.The test tube group is when level is vibrated, and instrument can produce significantly resonance, therefore when carrying out the structural design of high-flux tissue beveller, need evade the problem of resonance, and the frequency of vibration can not be provided with too high, this has seriously restricted the treatment effeciency of sample.
Safety detection for enetically modified food, in various test items, because the accuracy of detection of nucleic acids is the highest, therefore the safety detection project that is provided with mainly is that nucleic acid to the genetically modified plants of its raw-food material detects, promptly need smudge cells, extraction nucleic acid to test, this just causes the detection limit of food inspection mechanism very big, and common high-flux tissue beveller can't satisfy its requirement.
In addition, with Retsch is the low-frequency degree high-flux tissue beveller of representative, the reason of grinding owing to low-frequency degree makes broken need chronic of histocyte, make histocyte not exclusively broken, because to return to normal temperature be very fast to histocyte temperature in process of lapping after liquid nitrogen is handled, therefore its processing time is organized the clasmatosis time above the best usually, this just makes liquid nitrogen handle sample, the extraction of clasmatosis process and nucleic acid can not organically combine, make screw top micro tube time-consuming and expense manpower in shifting the working environment process, had a strong impact on the processing speed of sample.In addition because liquid nitrogen is handled and clasmatosis between interval time very long, gesture utilize the environment of ultralow temperature smudge cells, these factors all make the extraction efficiency of nucleic acid reduce.
[summary of the invention]
The technical problem to be solved in the present invention is, at organizing beveller to have the inner defective that is easy to generate resonance in the prior art, can only take low-frequency oscillation to carry out sample process and cause, cause that sample process efficient is low, the incomplete problem of clasmatosis, a kind of high-flux tissue beveller is provided, it adopts the mode of vertical vibration and greatly reduces the problem of internal resonance, thereby can use high frequency oscillation to carry out sample process.
The technical solution adopted for the present invention to solve the technical problems is: construct a kind of high-flux tissue beveller, comprise base, be arranged on driven unit on the described base and oscillation component and drive the clamp assembly that produces vibration and clamping sample by described oscillation component, described oscillation component comprises the guide rod that is fixed on the described base, the support arm that vertically is slidingly arranged on the described guide rod and is driven by described driven unit, described support arm side is provided with the vertical slide rail that matches with described guide rod, and described clamp assembly is fixed on the described support arm.
According to the described high-flux tissue beveller of claim 1, it is characterized in that, described driven unit comprises high-frequency electric machines, by motor-driven bent axle, be arranged on crankshaft end and drive the roller of described support arm vibration.
In the described high-flux tissue beveller of the invention described above, described base is provided with the roller groove that is complementary with described roller, the bottom of described support arm be arranged on described roller groove directly over.
In the described high-flux tissue beveller of the invention described above, described clamp assembly comprises the I shape support that is fixed on the described support arm, be arranged on the described I shape support and ambulatory splint that liftable is regulated, be arranged on the adapter between described ambulatory splint and the I shape support, and described adapter is provided with the pilot hole of accommodating a plurality of sample tube.
In the described high-flux tissue beveller of the invention described above, described clamp assembly also comprises sample tube that matches with the pilot hole of described adapter and the mill ball that is placed in the described sample tube.
In the described high-flux tissue beveller of the invention described above, the material of described mill ball is stainless steel, chromium steel, zirconia or tungsten carbide; The diameter of described mill ball is 0.1-1.5 millimeter, 2 millimeters, 3 millimeters, 4 millimeters, 5 millimeters, 7 millimeters, 10 millimeters, 12 millimeters, 20 millimeters, 25 millimeters or 30 millimeters.
In the described high-flux tissue beveller of the invention described above, the material of described adapter is PE, PS, textile or resin or ELISA Plate or centrifuge tube.
In the described high-flux tissue beveller of the invention described above, the back up pad that described base comprises the base plate that is placed on the table top, be connected with described base plate by beam, the vibration damper plate that is connected with described back up pad by damping spring.
In the described high-flux tissue beveller of the invention described above, also comprise control desk, be provided with the circuit board of the described machine operation of control in the described control desk and be arranged on the knob that is used to adjust each circuit parameter on the described control desk.
In the described high-flux tissue beveller of the invention described above, described circuit board comprises power input unit, timing unit, driver element and performance element.
In the described high-flux tissue beveller of the invention described above, also comprise the protective cover that is located on the described base, described driven unit, oscillation component and clamp assembly are arranged between described protective cover and the base.
In the described high-flux tissue beveller of the invention described above, be provided with travel switch between described protective cover and the base, described travel switch is controlled described motor and is quit work when described protective cover breaks away from base.
Implement high-flux tissue beveller of the present invention, has following beneficial effect: comprise that by oscillation component is set the vertical slide rail that is provided with on guide rod and the support arm cooperatively interacts, make the clamp assembly in the vertical direction that is positioned on the support arm vibrate, the direction of vibration that is positioned at the sample tube on the clamp assembly also is vertical, be positioned at the invisible spectro Tang Zhu of shaking and produce high-frequency moving up and down with respect to invisible spectro sample, in vitro freezing sample and mill ball are collided mutually, realize the smudge cells tissue thereby the grinding shearing force of its generation and impact make the histocyte wall break rapidly.Because base is arranged on the table top, the vibration on the vertical direction is born by base, and its inside is not easy to produce resonance, thereby can bear the vibration of higher frequency, has significantly improved its treatment effeciency; In addition, owing to be not easy to produce resonance, the noise of its course of work is littler.
In general, implement high-flux tissue beveller of the present invention, have following concrete advantage:
1, adopts high frequency type vertical oscillation system that in vitro freezing sample was finished in the shortest time, improved clasmatosis efficient.
2, the adapter that uses on this instrument can directly be put into liquid nitrogen container and preserves or handle, and it is centrifugal also can to put into hydro-extractor, for whole extraction experimentation has been saved the time and reduced operation steps.
3, solved the problem that adapter can not directly be put into liquid nitrogen container, freezing processing and break process better be connected, crushing effect is better.
The invention will be further described below in conjunction with drawings and Examples.
[description of drawings]
Figure 1 shows that the outside drawing of high-flux tissue beveller preferred embodiment of the present invention;
Figure 2 shows that the structural representation of high-flux tissue beveller preferred embodiment of the present invention;
Figure 3 shows that the structural representation of driven unit in the high-flux tissue beveller preferred embodiment of the present invention;
Figure 4 shows that the structural representation of clamp assembly in the high-flux tissue beveller preferred embodiment of the present invention;
Figure 5 shows that the circuit diagram of circuit board in the high-flux tissue beveller preferred embodiment of the present invention.
[embodiment]
In high-flux tissue beveller of the present invention, comprise base 10, be arranged on driven unit 20 on the base and oscillation component 30 and drive the clamp assembly 40 that produces vibration and clamping sample by oscillation component.Wherein, oscillation component comprises the guide rod 31 that is fixed on the base, the support arm 32 that vertically is slidingly arranged on the guide rod 31 and is driven by driven unit, and support arm 32 sides are provided with the vertical slide rail 33 that matches with guide rod 31, and clamp assembly is fixed on the support arm 32.When driven unit drove support arm 32 in the oscillation component along guide rod 31 in the vertical direction high-frequency to-and-fro movements, the sample of the clamping in the clamp assembly produced vibration, thereby reaches the effect of vibration smudge cells.
Shown in Fig. 1,2,3,4, in the preferred embodiment of high-flux tissue beveller of the present invention, mainly comprise base 10, driven unit 20, oscillation component 30, clamp assembly 40, control desk 51 (see figure 1)s and housing 52 (see figure 1)s.Its middle shell 52 is accommodated in all component wherein, and housing 5252 mainly is divided into two parts: control area and operating area, control area are provided with control desk 51, are used for setup parameter, operation and display parameter; The operating area comprises base 10, driven unit 20, oscillation component 30 and clamp assembly 40, is mainly used in sample is carried out break process.Preferably in control desk 51, be provided with the circuit board of the described machine operation of control and be arranged on the knob that is used to adjust each circuit parameter on the described control desk 51, as speed governing knob 4, start key 5, stop key 8 etc.; Also can various display device be set on control desk 51, be used to show working time, running parameter etc., as real-time grinding frequency indicator gauge 6, time control table 7 etc.
In order to reduce the programming rate of the sample after liquid nitrogen is handled; to guarantee cytoclastic effect; preferably on base 10, be provided with protective cover 1 in this preferred embodiment; this protective cover 1 all is accommodated in driven unit 20, oscillation component 30 and clamp assembly 40 wherein, makes in driven unit 20, oscillation component 30 and clamp assembly 40 enclosure space between protective cover 1 and base 10.Need put into or when taking out sample, can open this protective cover 1 and operate; When vibrating the break process process, then protective cover 1 can be covered.Can between protective cover 1 and base 10 safety lock be set, protective cover 1 is opened by mistake and is opened when being placed on instrument work.
As shown in Figure 1; in the preferred embodiment of high-flux tissue beveller of the present invention, can preferably on housing 52, be provided with protective cover handle 1, gas spring support 2, speed governing knob 4, start key 5, grind frequency indicator gauge 6, time control table 7, stop key 8, fan mouth 9, external power supply mouth 10, power switch 11 etc. in real time.
In this preferred embodiment, oscillation component 30 comprises guide rod 31 and support arm 32, and wherein guide rod 31 vertically is arranged on the base 10, and support arm 32 is provided with vertical slide rail 33, this vertical slide rail 33 matches with guide rod 31, makes support arm 32 to slide up and down along guide rod 31.
Shown in Fig. 2,3, in this preferred embodiment, driven unit 20 comprises high-frequency electric machines 21, bent axle (not shown) and roller 23.Wherein roller 23 is arranged on crankshaft end, is used to drive support arm 32 and produces vibration up and down; High-frequency electric machines 21 can drive bent axle by belt 21a and rotate, and makes the roller 23 that is positioned at crankshaft end produce eccentric rotary.Particularly, a crankshaft base 24 can be set on base, bent axle is arranged in this crankshaft base 24 by the bearing rotation; And expose at the two ends of bent axle and respectively at being provided with belt pulley and roller 23.The roller groove 12 that is complementary with roller 23 preferably is set on base 10, the bottom of support arm 32 be positioned at roller groove 12 directly over.When roller 23 in roller groove 12 during eccentric rotary, roller 23 is jack-up support arm 32 repeatedly, makes support arm 32 upwards slide along guide rail; When roller 23 returns to extreme lower position, support arm 32 at the effect lower edge of self gravitation guide rail to lower slider.So just can realize utilizing driven unit 20 driving arm arms 32 to pump, produce the vibration on the vertical direction, its oscillation frequency is by the rotating speed decision of motor 21.
As shown in Figure 4, in this preferred embodiment, clamp assembly 40 comprises support 41, ambulatory splint 42 and adapter 43.Its medium-height trestle 41 is fixedly installed on the support arm 32, preferably support 41 is set to I shape, and this I shape support 41 comprises top plate 41a, bottom plate 41b and middle connecting plate 41c.Ambulatory splint 42 is arranged on support top plate 41a below by the screw connection, and adapter 43 is arranged between ambulatory splint 42 and the bracket bottom laminate 41b.Set screw can be regulated the position of ambulatory splint 42, thereby the adapter 43 of different size can be clamped between the bottom plate 41b of ambulatory splint 42 and support.
In this preferred embodiment, clamp assembly 40 also can comprise special-purpose sample tube and be placed on the interior mill ball (not shown) of sample tube; Wherein sample tube matches with the pilot hole of adapter 43.Also can use the test tube of standard to come the splendid attire sample as required, the pilot hole that this moment need adapter 43 is set to standard size, also can be equipped with a plurality of adapters 43 with beveller according to different sizes, to adapt to not isometric test tube; And then detection limit is per sample selected different sample tubes and adapter 43 for use.Wherein the material of mill ball can be stainless steel, chromium steel, zirconia or tungsten carbide etc.; The diameter of mill ball can be set to 0.1-1.5 millimeter, 2 millimeters, 3 millimeters, 4 millimeters, 5 millimeters, 7 millimeters, 10 millimeters, 12 millimeters, 20 millimeters, 25 millimeters or 30 millimeters, can select the mill ball of different-diameter and material according to the thickness of cell.
Fig. 5 is a board circuit synoptic diagram of the present invention.Described circuit board comprises power input unit, timing unit, driver element and performance element.Wherein power input unit A, B comprise single-phase alternating current input, double-pole (band safety apparatus) gauge tap of AC220V 50Hz, also can comprise the pilot lamp whether power supply is connected.
Timing unit C preferably is set comprises time switch, timer and display screen.Wherein display screen can dynamically show the time of timer, can be excess time, also can be the time of having worked.Time set scope in the timer can be 0.001 second to 9999 hours multiple combination.Dynamically Show Color, static Show Color can freely be set by three looks, have the outage memory function, and integrated relay is done output control.
Driver element D comprises that 400W direct current generator 21 and DC motor speed-regulating drive plate and speed show composition; Pilot relay work when timer picks up counting; finish for motor 21 power supplies until timing by the relay energized; if in normal course of operation; when throwing open protective cover 1; be arranged on travel switch control stopping by force motor 21 work on the protective cover 1, timing pause TP is until building protective cover 1; then continue the front timing, motor 21 works on to this timing end.Have instant speed governing arbitrarily and the instant dynamically function of display speed when work.
The unlike signal that performance element E can provide according to the upstream is carried out different functions.
During use, connect the 220V power supply, turn on the power switch; Rotation speed governing knob generally about 1800 times/min, according to the difference of sample material and quantity, is done suitable adjustment to suitable vibration frequency; Sample for large volume is assurance grinding efficiency and quality, and the preliminary little processing of cutting is done in suggestion earlier; The sample of small size is packed in the 1.5/2.0mL sample tube (grind pipe), and sample size is controlled within the 1.5mL, and adds a mill ball or several mill balls; Each sample tube is put into the adapter 43 of suitable flux, is to guarantee balance, and the position of sample tube in two adapters 43 should symmetry.In sample size after a little while, can use blank pipe to do balance; The adapter 43 that will have sample was put into liquid nitrogen freezing several minutes, and general sample process according to the difference of sample material and quantity, is done suitable adjustment in 10min; Freezing back adapter 43 is placed on the fixture mount 41, adjusts and the fixed position; Cover protective cover 1 and lock the safety lock of protective cover 1, this moment, the vibration frequency indicator gauge was shown as 0000 in real time; Press " START " button of control desk 51, instrument is carried out and is ground shattering process, and the vibration frequency indicator gauge shows current vibration frequency in real time; The milling time of time control table display setting, and the milling time of instrument actual motion.In process of lapping, can adopt the operation that stops ending instrument by " STOP ", milling time makes zero; If directly open safety door, instrument is out of service equally, but the milling time non-return-to-zero, cover safety door after, grind and continue, the time control table continues the calculating of time last time; After treating that actual milling time arrives predetermined milling time, instrument is out of service automatically; Open protective cover 1 and safety lock, unclamp the ambulatory splint 42 of clamp assembly 40, take out adapter 43; Take out sample tube, process of lapping finishes, and takes out sample and carries out next step experiment.Handle 96 plant samples the whole process of good sample from intact tissue samples to fragmentation, generally in 10 minutes, finish.The plant tissue DNA that broken good sample adopts FAVORGEN company to provide extracts kit and extracts DNA, and sample DNA extracts up-to-standard rate 95.8% (carrying out statistical study DNA extraction quality with mensuration OD260nm and OD280nm value behind the extraction DNA).
Contrast Retsch beveller carries out same sample amount (96 s') clasmatosis, handle the back from liquid nitrogen and need about 30 minutes (needing each sample individual processing) to the intact time of clasmatosis, adopt identical kit to extract DNA, it is 80.8% that sample DNA extracts up-to-standard rate.
Back up pad 14 that base 10 comprises the base plate 13 that is placed on the table top, is connected with described base plate by beam 13a preferably is set in the above-described embodiments, passes through the vibration damper plate 16 that damping spring 15 is connected with described back up pad, thereby receive the internal resonance of instrument by damping spring, reduce noise.
Above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.
Claims (12)
1. high-flux tissue beveller, comprise base, be arranged on driven unit on the described base and oscillation component and drive the clamp assembly that produces vibration and clamping sample by described oscillation component, it is characterized in that, described oscillation component comprises the guide rod that is fixed on the described base, the support arm that vertically is slidingly arranged on the described guide rod and is driven by described driven unit, described support arm side is provided with the vertical slide rail that matches with described guide rod, and described clamp assembly is fixed on the described support arm.
2. according to the described high-flux tissue beveller of claim 1, it is characterized in that, described driven unit comprises high-frequency electric machines, by motor-driven bent axle, be arranged on crankshaft end and drive the roller of described support arm vibration.
3. according to the described high-flux tissue beveller of claim 2, it is characterized in that described base is provided with the roller groove that is complementary with described roller, the bottom of described support arm be arranged on described roller groove directly over.
4. according to the described high-flux tissue beveller of claim 1, it is characterized in that, described clamp assembly comprises the I shape support that is fixed on the described support arm, be arranged on the described I shape support and ambulatory splint that liftable is regulated, be arranged on the adapter between described ambulatory splint and the I shape support, and described adapter is provided with the pilot hole of accommodating a plurality of sample tube.
5. according to the described high-flux tissue beveller of claim 4, it is characterized in that described clamp assembly also comprises sample tube that matches with the pilot hole of described adapter and the mill ball that is placed in the described sample tube.
6. according to the described high-flux tissue beveller of claim 5, it is characterized in that the material of described mill ball is stainless steel, chromium steel, zirconia or tungsten carbide; The diameter of described mill ball is 0.1-1.5 millimeter, 2 millimeters, 3 millimeters, 4 millimeters, 5 millimeters, 7 millimeters, 10 millimeters, 12 millimeters, 20 millimeters, 25 millimeters or 30 millimeters.
7. according to the described high-flux tissue beveller of claim 4, it is characterized in that the material of described adapter is PE, PS, textile or resin or ELISA Plate or centrifuge tube.
8. according to each described high-flux tissue beveller in the claim 1 to 7, it is characterized in that the back up pad that described base comprises the base plate that is placed on the table top, be connected with described base plate by beam, the vibration damper plate that is connected with described back up pad by damping spring.
9. described according to Claim 8 high-flux tissue beveller is characterized in that, also comprises control desk, is provided with the circuit board of the described machine operation of control in the described control desk and is arranged on the knob that is used to adjust each circuit parameter on the described control desk.
10. according to the described high-flux tissue beveller of claim 9, it is characterized in that described circuit board comprises power input unit, timing unit, driver element and performance element.
11. described according to Claim 8 high-flux tissue beveller is characterized in that, also comprises the protective cover that is located on the described base, described driven unit, oscillation component and clamp assembly are arranged between described protective cover and the base.
12., it is characterized in that according to the described high-flux tissue beveller of claim 11, be provided with travel switch between described protective cover and the base, described travel switch is controlled described motor and is quit work when described protective cover breaks away from base.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2124060U (en) * | 1992-05-16 | 1992-12-09 | 河北大学 | Mechanical oscillator |
| JP2006320888A (en) * | 2005-04-20 | 2006-11-30 | Michio Shibatani | Crushing method, crusher using the method, and crushing apparatus |
| CN1940055A (en) * | 2005-09-29 | 2007-04-04 | 希森美康株式会社 | Sample preparation kit, sample preparation container, and sample processing device |
| US20090053689A1 (en) * | 2005-07-19 | 2009-02-26 | Attogenix Biosystems Pte. Ltd | Device for processing a biological and/or chemical sample and method of using the same |
| CN101650274A (en) * | 2009-08-31 | 2010-02-17 | 宁波新芝生物科技股份有限公司 | High pass tissue grinder and grinding method |
| WO2010026657A1 (en) * | 2008-09-06 | 2010-03-11 | 平田機工株式会社 | Sample crushing device |
| CN201532327U (en) * | 2009-04-07 | 2010-07-21 | 中国疾病预防控制中心营养与食品安全所 | Multifunctional histiocyte grinder |
| CN101852695A (en) * | 2010-04-30 | 2010-10-06 | 宁波新芝生物科技股份有限公司 | High-flux tissue grinder |
-
2011
- 2011-06-21 CN CN2011101683562A patent/CN102288462B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2124060U (en) * | 1992-05-16 | 1992-12-09 | 河北大学 | Mechanical oscillator |
| JP2006320888A (en) * | 2005-04-20 | 2006-11-30 | Michio Shibatani | Crushing method, crusher using the method, and crushing apparatus |
| US20090053689A1 (en) * | 2005-07-19 | 2009-02-26 | Attogenix Biosystems Pte. Ltd | Device for processing a biological and/or chemical sample and method of using the same |
| CN1940055A (en) * | 2005-09-29 | 2007-04-04 | 希森美康株式会社 | Sample preparation kit, sample preparation container, and sample processing device |
| WO2010026657A1 (en) * | 2008-09-06 | 2010-03-11 | 平田機工株式会社 | Sample crushing device |
| CN201532327U (en) * | 2009-04-07 | 2010-07-21 | 中国疾病预防控制中心营养与食品安全所 | Multifunctional histiocyte grinder |
| CN101650274A (en) * | 2009-08-31 | 2010-02-17 | 宁波新芝生物科技股份有限公司 | High pass tissue grinder and grinding method |
| CN101852695A (en) * | 2010-04-30 | 2010-10-06 | 宁波新芝生物科技股份有限公司 | High-flux tissue grinder |
Non-Patent Citations (1)
| Title |
|---|
| 《实验室研究与探索》 20101130 陈建军等 低电压细胞破碎的微型装置设计与工艺 7-9 1-12 第29卷, 第11期 * |
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