CN204211741U - Beveller - Google Patents

Abstract

The utility model discloses a kind of beveller, comprise base, be arranged on driven unit and the oscillation component on described base and driven to produce by described oscillation component and vibrate and the clamp assembly of grasping tissue sample, described base is provided with multiple transit bolt, described transit bolt comprises the threaded stud of tool, stud outwards evenly separates four support blades, the tread surface of a pair support blade is provided with friction layer, and another is provided with teeth layer to the tread surface of support blade; Each support blade is equipped with antirattle spring, and the upper end of this antirattle spring contacts susceptor surface under transit bolt tightening state.The utility model increases transit bolt and is used in transportation the protection of instrument.Support blade with friction layer rubs for increasing on shiny surface, prevents the movement of instrument in transportation.Support blade with teeth layer is used for increasing stability in slightly soft ground, prevents the movement of instrument in transportation.

Description

Beveller

Technical field

The utility model relates to a kind of bio-science research instrument, particularly relates to a kind of beveller.

Background technology

Cell breaking technology refers to and utilizes outside destroy cytolemma and cell walls, the technology making entocyte comprise object product component to discharge, and is the basis of the nonsecreting type biochemical substances (product) of synthesis in separation and purification cell.Develop various kinds of cell breaking method at present, to adapt to different purposes and the fragmentation of dissimilar cell walls.Breaking method can be advised and be received as mechanical process and the large class of on-mechanical method two.Mechanical process comprises high-pressure homogenization crush method, the Tang Zhu that shakes hits crush method, the grinding of high-speed stirring pearl crush method, ultrasonic fragmentation; On-mechanical method comprises osmotic shock crush method, freeze thawing crush method, the molten crush method of enzyme, chemically fragmenting method, washing agent crush method.

The on-mechanical method general broken time is long, is not suitable for the rapid detection aspect of sample.Mechanical process general operation is relatively more violent, and wherein high-pressure homogenization crush method and high-speed stirring pearl grinding crush method are mainly used in the industrial extraction to various material.Ultrasonic fragmentation, due to the restriction of equipment, is applied to industrial fewer.It is that isopyknic a small amount of tissue samples and highdensity mill ball (ZircoBeads) are put into sealable 2ml screw-cap micro tube that the Tang Zhu that shakes hits crush method, then adds damping fluid and stable elements to 1.The volume of 5ml, with 6500rpm tissue grinder instrument vibration at high speed 8 seconds, has a rest 8 seconds, then vibrates 8 seconds.This method is at present the soonest and once can process the method for maximum sample---machine at most can at process in a day 2400 samples, and this, but then cannot satisfy the demand time larger for treatment capacity concerning process in a small amount and multiple sample easily.

The beveller that the Tang Zhu that shakes hits crush method mainly in vitro adds the Tang Zhu that shakes what be equipped with sample, put into after test tube group is carried out liquid nitrogen process on instrument and carry out horizontal oscillations, to shake, Tang Zhuneng breaks up cell tissue in vitro, reaches the object extracting its inner nucleic acid.Test tube group is when horizontal oscillations, and instrument can produce and significantly resonate, and therefore when carrying out the structure design of beveller, needs the problem of evading resonance, and vibration frequency can not arrange too high, this seriously constrains the processing efficiency of sample.

For the safety detection of genetically modified food, in various test item, accuracy due to detection of nucleic acids is the highest, therefore the safety detection project arranged mainly detects the nucleic acid of the transgenic plant of its food raw material, namely need smudge cells, extract nucleic acid test, this just causes the detection limit of food inspection mechanism very large, and common beveller cannot meet its requirement.In addition, take Retsch as the low-frequency degree beveller of representative, it is chronic that reason due to low-frequency degree grinding makes histocyte fragmentation need, make histocyte not exclusively broken, because histocyte temperature return in process of lapping is very fast to normal temperature after liquid nitrogen process, therefore its treatment time exceedes the best histocyte broken time usually, this just makes liquid nitrogen process sample, the extraction of cytoclasis process and nucleic acid can not organically combine, make screw-cap micro tube time-consuming and expense manpower in transfer Working environment process, have a strong impact on the processing speed of sample.In addition because interval time between liquid nitrogen process and cytoclasis is very long, gesture utilizes the environment of very low temperature smudge cells, and these factors all make the extraction efficiency of nucleic acid reduce.

Utility model content

For the problems referred to above, the purpose of this utility model is to provide a kind of high efficiency beveller.

For achieving the above object, a kind of beveller described in the utility model, comprise base, be arranged on driven unit and the oscillation component on described base and driven to produce by described oscillation component and vibrate and the clamp assembly of grasping tissue sample, described oscillation component comprises the guide rod be fixed on described base, is vertically slidably arranged in the supported arm driven on described guide rod and by described driven unit, wherein said supported arm side is provided with the vertical slide rail matched with described guide rod, and described clamp assembly is fixed on described supported arm; Described driven unit comprises high-frequency electric machines, by motor-driven bent axle, is arranged on crankshaft end and drives the roller that described supported arm vibrates; Described clamp assembly comprises the I-shaped bracket be fixed on described supported arm, be arranged on described I-shaped bracket and liftable regulate ambulatory splint, be arranged on adapter between described ambulatory splint and I-shaped bracket, wherein said adapter is provided with the pilot hole of accommodating multiple sample tube; The back up pad that described base comprises the base plate be placed on table top, be connected with described base plate by cushion blocking, the vibration damper plate be connected with described back up pad by shock spring; Described base is provided with multiple transit bolt, described transit bolt comprises the threaded stud of tool, stud outwards evenly separates four support blades, and the tread surface of a pair support blade is provided with friction layer, and another is provided with teeth layer to the tread surface of support blade; Each support blade is equipped with antirattle spring, and the upper end of this antirattle spring contacts susceptor surface under transit bolt tightening state.

Preferably, described base is provided with the roller groove matched with described roller, and the bottom of described supported arm is arranged on directly over described roller groove.

Preferably, described clamp assembly comprises the sample tube matched with the pilot hole of described adapter and the mill ball be placed in described sample tube.

Preferably, also comprise the protective guard be located on described base, described driven unit, oscillation component and clamp assembly are arranged between described protective guard and base.

The beneficial effects of the utility model are:

1, adopt high frequency type vertical oscillation system that in vitro freezing sample can be made to complete in the shortest time, improve cytoclasis efficiency.

2, the adapter this instrument used directly can be put into liquid nitrogen container and carry out preserving or processing, and also can put into whizzer centrifugal, saves the time and reduce operation steps for whole extraction experimentation.

3, solve the problem that adapter directly can not put into liquid nitrogen container, freezing treatment and break process are better connected, and crushing effect is better.

4, increase transit bolt to be used in transportation the protection of instrument.Support blade with friction layer rubs for increasing on shiny surface, prevents the movement of instrument in transportation.Support blade with teeth layer is used for increasing stability in slightly soft ground, prevents the movement of instrument in transportation.

Accompanying drawing explanation

Figure 1 shows that the outside view of beveller described in the utility model embodiment;

Figure 2 shows that the structural representation of beveller described in the utility model embodiment;

Figure 3 shows that the structural representation of driven unit in beveller described in the utility model embodiment;

Figure 4 shows that the structural representation of clamp assembly in beveller described in the utility model embodiment;

Figure 5 shows that the schematic circuit of circuit card in beveller described in the utility model embodiment;

Figure 6 shows that beveller base view described in the utility model embodiment;

Figure 7 shows that the sectional view of transit bolt on base described in the utility model embodiment;

Figure 8 shows that the orthographic plan of transit bolt.

Embodiment

Below in conjunction with Figure of description, the utility model is further described.

In beveller described in the utility model, comprise base 10, be arranged on driven unit 20 and the oscillation component 30 on base and driven to produce by oscillation component and vibrate and the clamp assembly 40 of clamping sample.Wherein, oscillation component comprises the guide rod 31 be fixed on base, is vertically slidably arranged in the supported arm 32 driven on guide rod 31 and by driven unit, and supported arm 32 side is provided with the vertical slide rail 33 matched with guide rod 31, and clamp assembly is fixed on supported arm 32.When driven unit drives the supported arm 32 in oscillation component to move along guide rod 31 in the vertical direction high-frequent reciprocating, the sample of the clamping in clamp assembly produces vibration, thus reaches the effect of vibration smudge cells.

As shown in Figure 1,2,3, 4, in the preferred embodiment of beveller described in the utility model, mainly comprise base 10, driven unit 20, oscillation component 30, clamp assembly 40, supervisory control desk 51 (see Fig. 1) and housing 52 (see Fig. 1).

All component is accommodated in wherein by its middle shell 52, and housing 5252 is mainly divided into two parts: control area and operating area, and control area is provided with supervisory control desk 51, for setup parameter, operation and display parameter; Operating area comprises base 10, driven unit 20, oscillation component 30 and clamp assembly 40, is mainly used in carrying out break process to sample.In supervisory control desk 51, being preferably provided with the circuit card controlling described machine operation and being arranged on for adjusting the knob of each circuit parameter on described supervisory control desk 51, as speed governing knob 4, start key 5, stop key 8 etc.; Also various display unit can be set on supervisory control desk 51, for showing working hour, working parameter etc., as ground frequency indicator gauge 6, time controling table 7 etc. in real time.

In order to reduce the heat-up rate of the sample after liquid nitrogen process; to ensure cytoclastic effect; preferably on base 10, protective guard 1 is provided with in this preferred embodiment; driven unit 20, oscillation component 30 and clamp assembly 40 are all accommodated in wherein by this protective guard 1, make in driven unit 20, oscillation component 30 and the enclosed space of clamp assembly 40 between protective guard 1 and base 10.When needing put into or take out sample, this protective guard 1 can be opened and operate; When carrying out vibration break process process, then protective guard 1 can be covered.Can arrange safety lock between protective guard 1 and base 10, when being placed on instrument work, protective guard 1 is opened by mistake and is opened.

As shown in figs 6-8, described base 10 is provided with multiple transit bolt 60, described transit bolt comprises the threaded stud 601 of tool, stud outwards evenly separates four support blades 602, the tread surface of a pair support blade is provided with friction layer 604, and another is provided with teeth layer 605 to the tread surface of support blade; Each support blade is equipped with antirattle spring 603, and the upper end of this antirattle spring contacts susceptor surface under transit bolt tightening state.Transit bolt is used in transportation the protection of instrument.Support blade with friction layer rubs for increasing on shiny surface, prevents the movement of instrument in transportation.Support blade with teeth layer is used for increasing stability in slightly soft ground, prevents the movement of instrument in transportation.

As shown in Figure 1; in the preferred embodiment of beveller described in the utility model, preferably can be provided with protective guard handle 1, gas spring support 2, speed governing knob 4, start key 5, in real time grinding frequency indicator gauge 6, time controling table 7, stop key 8, fan mouth 9, external source mouth 10, power switch 11 etc. on housing 52.

In the preferred embodiment, oscillation component 30 comprises guide rod 31 and supported arm 32, and wherein guide rod 31 is vertically arranged on base 10, and supported arm 32 is provided with vertical slide rail 33, this vertical slide rail 33 matches with guide rod 31, and supported arm 32 can be slided up and down along guide rod 31.

As shown in Figure 2,3, in the preferred embodiment, driven unit 20 comprises high-frequency electric machines 21, bent axle (not shown) and roller 23.Wherein roller 23 is arranged on crankshaft end, vibrates up and down for driving supported arm 32 to produce; High-frequency electric machines 21 rotates by belt 21a driving crank, makes the roller 23 being positioned at crankshaft end produce eccentric rotary.Particularly, a crankshaft base 24 can be set on base, bent axle be rotated by bearing and is arranged in this crankshaft base 24; And the two ends of bent axle are exposed and respectively at being provided with belt pulley and roller 23.On base 10, preferably arrange the roller groove 12 matched with roller 23, the bottom of supported arm 32 is positioned at directly over roller groove 12.When roller 23 is in roller groove 12 during eccentric rotary, roller 23 can jack-up supported arm 32 repeatedly, makes supported arm 32 along guide rail upward sliding; When roller 23 returns to extreme lower position, supported arm 32 under the effect of self gravitation along guide rail slide downward.So just can realize utilizing driven unit 20 driving arm arm 32 to pump, produce the vibration on vertical direction, its oscillation frequency is determined by the rotating speed of motor 21.

As shown in Figure 4, in the preferred embodiment, clamp assembly 40 comprises support 41, ambulatory splint 42 and adapter 43.Its medium-height trestle 41 is fixedly installed on supported arm 32, preferably support 41 is set to I shape, and this I-shaped bracket 41 comprises top plate 41a, bottom plate 41b and middle connecting plate 41c.Ambulatory splint 42 is connected by screw and is arranged on below support top plate 41a, and adapter 43 is arranged between ambulatory splint 42 and bracket bottom laminate 41b.Set screw can regulate the position of ambulatory splint 42, thus between the bottom plate 41b that the adapter 43 of different size can be clamped in ambulatory splint 42 and support.

Adapter 43 is mainly used for the test tube loading multiple band sample, due to before carrying out break process, the test tube that sample is housed is needed to carry out liquid nitrogen process, if mobile test tube one by one again after processing, operating on the one hand, it is time-consuming to bother very much, makes the temperature of sample can return to normal temperature rapidly on the other hand and misses the optimum cell broken time.Preferably arrange adapter 43 and comprise pedestal 43a and lid 43b two portions, wherein pedestal 43a and lid 43b corresponding position are equipped with multiple counterbore matched with sample tube.During use, many sample tube are inserted in the counterbore of adapter pedestal 43a, then be enclosed within sample tube with the counterbore that lid 43b is corresponding, so just sample tube is positioned between pedestal 43a and lid 43b.Whole adapter 43 can load many sample tube simultaneously, realizes batch processing.Preferred adapter 43 adopts the material directly can putting into liquid nitrogen container to make, as PE, PS, textiles or resin or enzyme plate or centrifuge tube etc.; Like this when carrying out liquid nitrogen process, can directly the adapter 43 that sample tube is housed be carried out preserving or processing; And this adapter 43 directly can be fixed on beveller described in the utility model by clamp assembly 40 support 41 on carry out broken wall treatment, avoid mobile test tube one by one.

In the preferred embodiment, clamp assembly 40 also can comprise special sample tube and be placed on the mill ball (not shown) in sample tube; Wherein sample tube matches with the pilot hole of adapter 43.Also the test tube of standard can be used as required to carry out splendid attire sample, now need the pilot hole of adapter 43 to be set to standard size, also can be equipped with multiple adapter 43 according to different sizes with beveller, to adapt to not isometric test tube; And then detection limit per sample selects different sample tubes and adapter 43.Wherein the material of mill ball can be stainless steel, chromium steel, zirconium white or wolfram varbide etc.; The diameter of mill ball can be set to 0.1-1.5 millimeter, 2 millimeters, 3 millimeters, 4 millimeters, 5 millimeters, 7 millimeters, 10 millimeters, 12 millimeters, 20 millimeters, 25 millimeters or 30 millimeters, the mill ball of different diameter and material can be selected according to the thickness of cell.

Fig. 5 is board circuit schematic diagram of the present utility model.Described circuit card comprises power input unit, timing unit, driver element and actuator unit.Wherein power input unit A, B comprise single phase alternating current input, double-pole (band safety interlock) trip switch of AC220V 50Hz, also can comprise the pilot lamp whether power supply is connected.

Timing unit C is preferably set and comprises time switch, timing register and display screen.Wherein the time of display screen dynamic display timing register, can be remaining time, also can be the time worked.Time setting range in timing register can be the multiple combination of 0.001 second to 9999 hours.Dynamic Announce color, static state display color can three look free settings, have power failure memory function, the dry points output-controlling of integrated relay.

Driver element D comprises 400W direct-current machine 21 and DC motor speed-regulating drive plate and speed display and forms; Relay work while timing register starts timing; being switched on power by rly. powers until timing terminates to motor 21; if in normal course of operation; when throwing open protective guard 1; the travel switch be arranged on protective guard 1 controls to stop motor 21 to work by force, and timing pause TP, until build protective guard 1; then continue timing above, motor 21 works on to this timing and terminates.There is the function of operationally instant speed governing and instant Dynamic Announce speed arbitrarily.The unlike signal that actuator unit E can provide according to upstream, performs different functions.

During use, connect 220V power supply, turn on the power switch; Rotate speed governing knob to suitable vibrational frequency, generally at 1800 times/about min, according to the difference of sample material and quantity, do suitable adjustment; Sample for large volume is guarantee mill efficiency and quality, and preliminary little process of cutting first is done in suggestion; Loaded by the sample of small volume in 1.5/2.0mL sample tube (grinding pipe), sample size controls within 1.5mL, and adds a mill ball or several mill balls; Each sample test tube is put into the adapter 43 of suitable flux, for ensureing balance, the position of sample tube in two adapters 43 should be symmetrical.When sample size is few, blank pipe can be used to balance; Adapter 43 with sample is put into liquid nitrogen freezing several minutes, and general sample process, in 10min, according to the difference of sample material and quantity, does suitable adjustment; Freezing rear adapter 43 is placed in fixture mount 41, adjusts and fixed position; Cover protective guard 1 and lock the safety lock of protective guard 1, now vibrational frequency indicator gauge is shown as 0000 in real time; Press " START " button of supervisory control desk 51, instrument performs grinding shattering process, and real-time vibrational frequency indicator gauge shows current vibrational frequency; The milling time of time controling table display-setting, and the milling time of instrument actual motion.In process of lapping, can adopt the operation stopping stopping instrument by " STOP ", milling time makes zero; If directly open relief door, instrument is out of service equally, but milling time non-return-to-zero, and after covering relief door, grinding continues, and time controling table continues the calculating of time last time; Arrive after predetermined milling time until actual milling time, instrument is automatically out of service; Open protective guard 1 and safety lock, unclamp the ambulatory splint 42 of clamp assembly 40, take out adapter 43; Take out sample tube, process of lapping terminates, and takes out the experiment that sample carries out next step.From intact tissue samples to fragmentation good sample whole process process 96 plant samples, generally completed in 10 minutes.The plant tissue DNA that broken good sample adopts FAVORGEN company to provide extracts test kit and carries out extraction DNA, and sample DNA extracts up-to-standard rate 95.8% (carrying out statistical study DNA extraction quality to measure OD260nm and OD280nm value after extracting DNA).

Contrast Retsch beveller carries out the cytoclasis of same sample amount (96), about 30 minutes (needing each sample individual curing) is needed to the cytoclasis complete time from after liquid nitrogen process, adopt identical test kit to carry out extraction DNA, it is 80.8% that sample DNA extracts up-to-standard rate.

The back up pad 14 that base 10 is comprised the base plate 13 be placed on table top, is connected with described base plate by cushion blocking 13a, the vibration damper plate 16 be connected with described back up pad by shock spring 15 are preferably set in the above-described embodiments, thus the internal resonance of instrument is received by shock spring, reduce noise.

Above; be only preferred embodiment of the present utility model; but protection domain of the present utility model is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the utility model discloses; the change that can expect easily or replacement, all should be encompassed within protection domain of the present utility model.Therefore, the protection domain that protection domain of the present utility model should define with claim is as the criterion.

Claims (4)

1. a beveller, comprise base, be arranged on driven unit and the oscillation component on described base and driven to produce by described oscillation component and vibrate and the clamp assembly of grasping tissue sample, it is characterized in that, described oscillation component comprises the guide rod be fixed on described base, is vertically slidably arranged in the supported arm driven on described guide rod and by described driven unit, wherein said supported arm side is provided with the vertical slide rail matched with described guide rod, and described clamp assembly is fixed on described supported arm; Described driven unit comprises high-frequency electric machines, by motor-driven bent axle, is arranged on crankshaft end and drives the roller that described supported arm vibrates; Described clamp assembly comprises the I-shaped bracket be fixed on described supported arm, be arranged on described I-shaped bracket and liftable regulate ambulatory splint, be arranged on adapter between described ambulatory splint and I-shaped bracket, wherein said adapter is provided with the pilot hole of accommodating multiple sample tube; The back up pad that described base comprises the base plate be placed on table top, be connected with described base plate by cushion blocking, the vibration damper plate be connected with described back up pad by shock spring; Described base is provided with multiple transit bolt, described transit bolt comprises the threaded stud of tool, stud outwards evenly separates four support blades, and the tread surface of a pair support blade is provided with friction layer, and another is provided with teeth layer to the tread surface of support blade; Each support blade is equipped with antirattle spring, and the upper end of this antirattle spring contacts susceptor surface under transit bolt tightening state.

2. beveller according to claim 1, is characterized in that, described base is provided with the roller groove matched with described roller, and the bottom of described supported arm is arranged on directly over described roller groove.

3. beveller according to claim 1, is characterized in that, described clamp assembly comprises the sample tube matched with the pilot hole of described adapter and the mill ball be placed in described sample tube.

4. beveller according to claim 1, is characterized in that, also comprises the protective guard be located on described base, and described driven unit, oscillation component and clamp assembly are arranged between described protective guard and base.