CN108752094B - Compound pleurotus nebrodensis bud-inducing agent - Google Patents

Compound pleurotus nebrodensis bud-inducing agent Download PDF

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CN108752094B
CN108752094B CN201810593022.1A CN201810593022A CN108752094B CN 108752094 B CN108752094 B CN 108752094B CN 201810593022 A CN201810593022 A CN 201810593022A CN 108752094 B CN108752094 B CN 108752094B
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bud
pleurotus nebrodensis
compound
inducing agent
agent
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CN108752094A (en
Inventor
张红刚
郭霄飞
张程
郭尚
张勇
郭明慧
石建森
周伟
刘晶
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Institute Of Edible Fungi Shanxi Academy Of Agricultural Sciences
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Institute Of Edible Fungi Shanxi Academy Of Agricultural Sciences
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D5/00Fertilisers containing magnesium
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/10Fertilisers containing plant vitamins or hormones
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions

Abstract

The invention relates to the technical field of edible fungus cultivation, in particular to a compound pleurotus nebrodensis bud forcing agent which comprises brassinolide, potassium dihydrogen phosphate and magnesium sulfate. The bud promoting agent can regulate the mycelium culture period, improve the bud promoting condition of the pleurotus nebrodensis, improve the enzyme activity to achieve the effect of increasing the yield, save the link of low-temperature bud promoting, improve the bud rate, ensure even bud formation, is convenient to apply, reduces the cultivation difficulty, is suitable for the industrial production of the pleurotus nebrodensis and is suitable for popularization.

Description

Compound pleurotus nebrodensis bud-inducing agent
Technical Field
The invention relates to the technical field of edible fungus cultivation, in particular to a compound pleurotus nebrodensis bud promoter.
Background
Pleurotus nebrodensis is also called Pleurotus ferulae Lanzi and Pleurotus ferulae Lanzi. The pleurotus nebrodensis has tender meat, delicious taste and high medicinal value, is known as "bolete on grassland", can regulate physiological balance of human body and enhance immune function of human body, and its polysaccharide and organic extract can be used for preventing and treating cardiovascular diseases of old people, rickets of children, osteomalacia, osteoporosis and other diseases. The pleurotus nebrodensis belongs to rare edible fungi, is one of a few mushroom species with independent intellectual property rights in China, and has short large-scale cultivation time and high economic value.
The pleurotus nebrodensis mainly exists in the cultivation process: the bud emergence is uneven and low, namely, the fungus sticks in production have more buds but low survival rate, or have few buds, no mushroom and difficult control; secondly, compared with other mushroom species, the hypha of the pleurotus nebrodensis still needs a post-maturation period of more than 60 days after growing in a fungus bag, bud forcing is carried out at a low temperature of 3-8 ℃, failure is caused by high technical level requirements in production frequently, so that the industrial development of the pleurotus nebrodensis is restricted, and the problems in pleurotus nebrodensis production are researched and solved, so that the pleurotus nebrodensis industry is forced to be in the forefront.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a compound pleurotus nebrodensis bud promoting agent, which solves the problems that buds are not uniform, the bud rate is low, bud promotion needs to be carried out at low temperature and the like in the conventional pleurotus nebrodensis cultivation.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a compound Pleurotus nebrodensis bud inducing agent comprises brassinolide, potassium dihydrogen phosphate and magnesium sulfate, wherein the brassinolide, potassium dihydrogen phosphate and magnesium sulfate are mixed and prepared by adding water, and sprayed to the Pleurotus nebrodensis bud inducing stage.
The concentration of water added into the brassinolide is 0.01g/L, the concentration of potassium dihydrogen phosphate is 10g/L, and the concentration of magnesium sulfate is 5 g/L.
The room temperature of the bud forcing stage is 18-23 ℃.
The temperature of the bud inducing liquid for spraying is 10-15 ℃.
The bud forcing agent is sprayed under the following conditions: the effective accumulated temperature of the fungus stick reaches 1600 ℃.
The spraying method of the bud promoter comprises the following steps: opening the fungus stick, and spraying a bud promoting agent on the surface of the material.
The bud promoting agent is sprayed once every 10 hours, and is sprayed twice in total.
The amount of the bud forcing agent sprayed each time is 0.3-1.3 ml.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a compound pleurotus nebrodensis bud promoting agent, which is used in the bud promoting stage, so that the link of promoting buds at low temperature is omitted, and pleurotus nebrodensis can be cultivated in any season without cooling. The bud promoting agent can regulate the mycelium culture period, improve the bud promoting condition of the pleurotus nebrodensis, improve the enzyme activity to achieve the effect of increasing the yield, save the link of low-temperature bud promoting, improve the bud rate, ensure even bud formation, is convenient to apply, reduces the cultivation difficulty, is suitable for the industrial production of the pleurotus nebrodensis and is suitable for popularization.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A compound pleurotus nebrodensis bud forcing agent comprises brassinolide, monopotassium phosphate and magnesium sulfate, wherein the brassinolide, the monopotassium phosphate and the magnesium sulfate are mixed and added with water to prepare the bud forcing agent, and the bud forcing agent is sprayed to the pleurotus nebrodensis bud forcing stage. The concentration of the brassinolide is 0.01g/L, the concentration of the monopotassium phosphate is 10g/L, and the concentration of the magnesium sulfate is 5 g/L. The bud forcing stage is at room temperature of 18 ℃.
The spraying method of the bud promoter comprises the following steps: when the effective accumulated temperature of the fungus sticks reaches 1600 ℃, opening the fungus sticks, spraying a bud-promoting agent on the surface of the material, and controlling the temperature of the bud-promoting liquid to be 15 ℃. The bud promoter is sprayed once every 10h, and is sprayed twice in total, and the spraying amount is 1.3ml each time.
The growth condition of pleurotus nebrodensis after bud forcing by the bud forcing agent in the embodiment is as follows: the growth speed of hypha is 3.12mm/d, the bud ratio is 82.84%, the biological efficiency is 57.51%, the average yield is 221.16d, and the bud days are 113 +/-3.7 d.
Example 2
A compound pleurotus nebrodensis bud forcing agent comprises brassinolide, monopotassium phosphate and magnesium sulfate, wherein the brassinolide, the monopotassium phosphate and the magnesium sulfate are mixed and added with water to prepare the bud forcing agent, and the bud forcing agent is sprayed to the pleurotus nebrodensis bud forcing stage. The concentration of the brassinolide is 0.01g/L, the concentration of the monopotassium phosphate is 10g/L, and the concentration of the magnesium sulfate is 5 g/L. The bud forcing stage is at 23 ℃ at room temperature.
The spraying method of the bud promoter comprises the following steps: when the effective accumulated temperature of the fungus sticks reaches 1600 ℃, opening the fungus sticks, spraying a bud-promoting agent on the surface of the material, and controlling the temperature of the bud-promoting liquid to be 10 ℃. The bud promoter is sprayed once every 10h, and is sprayed twice, and the spraying amount is 1ml each time.
The growth condition of pleurotus nebrodensis after bud forcing by the bud forcing agent in the embodiment is as follows: the growth speed of hypha is 5.76mm/d, the bud ratio is 98.76%, the biological efficiency is high, the average yield is 251.16d, and the bud days are 108 +/-2.9 d.
Example 3
A compound pleurotus nebrodensis bud forcing agent comprises brassinolide, monopotassium phosphate and magnesium sulfate, wherein the brassinolide, the monopotassium phosphate and the magnesium sulfate are mixed and added with water to prepare the bud forcing agent, and the bud forcing agent is sprayed to the pleurotus nebrodensis bud forcing stage. The concentration of the brassinolide is 0.01g/L, the concentration of the monopotassium phosphate is 10g/L, and the concentration of the magnesium sulfate is 5 g/L. The bud forcing stage is at room temperature of 20 ℃.
The spraying method of the bud promoter comprises the following steps: when the effective accumulated temperature of the fungus sticks reaches 1600 ℃, opening the fungus sticks, spraying a bud-promoting agent on the surface of the material, and controlling the temperature of the bud-promoting liquid to be 13 ℃. The bud promoter is sprayed once every 10h, and is sprayed twice in total, and the spraying amount is 0.3ml each time.
The growth condition of pleurotus nebrodensis after bud forcing by the bud forcing agent in the embodiment is as follows: the growth speed of hypha is 3.75mm/d, the bud ratio is 81.34%, the biological efficiency is 56.32%, the average yield is 217.28d, and the bud-forming days are 115 +/-3.8 d.
Comparative example
The formula of the bud-promoting agent for spraying comprises: gibberellin 920 (GA) 0.01g/L + potassium dihydrogen phosphate 10g/L + magnesium sulfate 5 g/L; 0.01g/L of naphthylacetic acid (NAA), 10g/L of monopotassium phosphate and 5g/L of magnesium sulfate; brassinolide (BR)0.01g/L + potassium dihydrogen phosphate 10g/L + magnesium sulfate 5g/L and blank control.
After the pleurotus nebrodensis fungi bag is full, when the accumulated temperature reaches 1600 ℃, the room temperature is controlled to be 18-23 ℃, the bud promoting agent in the comparative example is sprayed on the opening material surface of the pleurotus nebrodensis fungi bag, 10ml of the bud promoting agent is sprayed every 10 hours, the spraying is carried out twice, and the growth condition of the pleurotus nebrodensis after the spraying is shown in table 1:
table 1: growth of Pleurotus nebrodensis under the action of different bud-promoting agents
Formulation of Gibberellin 920 (GA) 0.01g/L + phosphorus 10g/L of potassium dihydrogen acid + 5g/L of magnesium sulfate Naphthylacetic acid (NAA)0.01g/L + phosphoric acid 10g/L of potassium dihydrogen + 5g/L of magnesium sulfate Brassinolide (BR)0.01g/L + phosphorus 10g/L of potassium dihydrogen acid + 5g/L of magnesium sulfate Blank space (spraying of the spray 1ml)
Growth of hyphae Speed (mm- d) 5.76 4.51 5.76 3.14
Bud ratio (%) 87.54 82.84 98.76 73.32
Biological effect Percentage (%) 57.67 54.32 62.79 53.41
Average yield (g) 213.68 201.28 251.16 186.64
Days of bud formation (d) 112±5.3 122±1.8 108±2.9 121± 2.4
Growth of hyphae Potential energy *** *** **** **
Compared with a blank group, the bud promoting agent with different combinations can shorten the bud emergence days of fruiting and increase the unit yield by spraying, but the bud promoting agent cultured by brassinolide has the best effect, is shortened by nearly 13 days compared with a contrast, and has the best biological efficiency and product yield.
Although only the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art, and all changes are encompassed in the scope of the present invention.
The invention relates to the technical field of edible fungus cultivation, in particular to a compound pleurotus nebrodensis bud forcing agent which comprises brassinolide, potassium dihydrogen phosphate and magnesium sulfate. The bud promoting agent can regulate the mycelium culture period, improve the bud promoting condition of the pleurotus nebrodensis, improve the enzyme activity to achieve the effect of increasing the yield, save the link of low-temperature bud promoting, improve the bud rate, ensure even bud formation, is convenient to apply, reduces the cultivation difficulty, is suitable for the industrial production of the pleurotus nebrodensis and is suitable for popularization.

Claims (7)

1. The compound pleurotus nebrodensis bud inducing agent is characterized in that: the compound preparation is prepared by mixing brassinolide, monopotassium phosphate and magnesium sulfate, adding water, and spraying the mixture to a pleurotus nebrodensis bud forcing stage; the concentration of the brassinolide is 0.01g/L, the concentration of the monopotassium phosphate is 10g/L, and the concentration of the magnesium sulfate is 5 g/L.
2. The compound pleurotus nebrodensis bud inducing agent as claimed in claim 1, wherein: the room temperature of the bud forcing stage is 18-23 ℃.
3. The compound pleurotus nebrodensis bud inducing agent as claimed in claim 1, wherein: the temperature of the bud inducing liquid for spraying is 10-15 ℃.
4. The compound pleurotus nebrodensis bud inducing agent as claimed in claim 1, wherein: the bud forcing agent is sprayed under the following conditions: the effective accumulated temperature of the fungus stick reaches 1600 ℃.
5. The compound pleurotus nebrodensis bud inducing agent according to claim 1, wherein the spraying method of the bud inducing agent comprises the following steps: opening the fungus stick, and spraying a bud promoting agent on the surface of the material.
6. The compound pleurotus nebrodensis bud inducing agent according to claim 5, wherein the compound pleurotus nebrodensis bud inducing agent comprises the following components in percentage by weight: the bud promoting agent is sprayed once every 10 hours, and is sprayed twice in total.
7. The compound pleurotus nebrodensis bud inducing agent according to claim 6, wherein the compound pleurotus nebrodensis bud inducing agent comprises the following components in percentage by weight: the amount of the bud forcing agent sprayed each time is 0.3-1.3 ml.
CN201810593022.1A 2018-06-11 2018-06-11 Compound pleurotus nebrodensis bud-inducing agent Active CN108752094B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101434500A (en) * 2008-11-28 2009-05-20 浙江林学院 Fertilizer containing plant charcoals and preparation thereof
CN102875223A (en) * 2011-07-14 2013-01-16 湖北省烟草公司襄阳市公司 Leaf fertilizer special for tobacco
CN104177184A (en) * 2014-08-26 2014-12-03 万客齐食品(云南)有限公司 Artichoke foliage spraying fertilizer
CN106431619A (en) * 2016-08-31 2017-02-22 横县生产力促进中心 Jasmine foliar spray fertilizer promoting blossoms

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101434500A (en) * 2008-11-28 2009-05-20 浙江林学院 Fertilizer containing plant charcoals and preparation thereof
CN102875223A (en) * 2011-07-14 2013-01-16 湖北省烟草公司襄阳市公司 Leaf fertilizer special for tobacco
CN104177184A (en) * 2014-08-26 2014-12-03 万客齐食品(云南)有限公司 Artichoke foliage spraying fertilizer
CN106431619A (en) * 2016-08-31 2017-02-22 横县生产力促进中心 Jasmine foliar spray fertilizer promoting blossoms

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
不同生长调节剂处理白灵菇幼菇对产质量的影响;苟永平 等;《食用菌》;20031231;第7页右侧栏1.2节,第7页右侧栏倒数第1-4行,第8页左侧栏倒数第1-8行 *

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