CN108738776A - A kind of bi-annual planting method of STEVIA REBAUDIANA breeding - Google Patents
A kind of bi-annual planting method of STEVIA REBAUDIANA breeding Download PDFInfo
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- CN108738776A CN108738776A CN201810539597.5A CN201810539597A CN108738776A CN 108738776 A CN108738776 A CN 108738776A CN 201810539597 A CN201810539597 A CN 201810539597A CN 108738776 A CN108738776 A CN 108738776A
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- stevia rebaudiana
- breeding
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- cultivation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention belongs to STEVIA REBAUDIANA breeding technique, the bi-annual planting methods of STEVIA REBAUDIANA breeding.Existing STEVIA REBAUDIANA breeding work sows autumn harvest in spring, can only cultivate a season within 1 year, Breeding progress is slow.Step of the present invention is：Spring first makees greenhouse cuttage and seedling culture, open country field planting；First, which harvests the first 15 days economical characters and quality determination to STEVIA REBAUDIANA original material, investigates;It formulates second and makees Hybridizing plan;Second makees to cut stem section cuttage and seedling culture on the plant made from first；After seedling is bred as, it is colonized in flowerpot；Before blooming, STEVIA REBAUDIANA parents pairing is put together；Florescence insect pollination hybridizes；Autumn weather turns cool, and potting STEVIA REBAUDIANA is moved into vinyl house；Reciprocal cross seed is harvested after seed maturity respectively.It is an advantage of the invention that：Bi-annual planting is cultivated, and the STEVIA REBAUDIANA crossbreeding work period is reduced, and improves breeding efficiency, saves breeding cost.
Description
Technical field
The invention belongs to STEVIA REBAUDIANA breeding techniques, specifically STEVIA REBAUDIANA bi-annual planting breeding method.
Background technology
The STEVIA REBAUDIANA blade 6-12% of glycosides containing synanthrin, fine work are white powder, and sugariness is 250--450 times of sucrose, calorific value
It is the 1/300 of sucrose, is a kind of natural sweetener of sugariness low in calories, high.It is not absorbed, is not generated after synanthrin glycosides intake human body
Heat is diabetes and the sweetener that bariatric patients are applicable in, and is the important source material of food and pharmaceutical industries.It is widely used in sugar
In fruit, cake, beverage, solid beverage, fried pot foods, seasoning, preserved fruit.STEVIA REBAUDIANA quickly breeds sweetleaf in STEVIA REBAUDIANA industry
The kind of synanthrin glycosides quickly improves STEVIA REBAUDIANA output blade, for increasing the total output of stevioside, for improving STEVIA REBAUDIANA
The economic benefit of cultivation, for promoting human health to suffer from important role.
STEVIA REBAUDIANA different lines intermolecular hybrid is the important channel for cultivating STEVIA REBAUDIANA new varieties.Traditional STEVIA REBAUDIANA hybridization is educated
Kind, breeder every year will be according to breeding objective and existing each parent in upper 1 year and the performance in the generation earlier, system
Determine Hybridizing plan, carries out crossbreeding work.It forms the previous year observation, compare parent, the Working mould that latter year is hybridized
Formula.Crossing work can only be carried out in the next year after observing, comparing parent in this operating mode, and whole process needs 2 years
Time.Two stages of the work of above-mentioned crossbreeding are closed and are carried out in same year by the present invention, by being used for for previous latter year
The cultivation work of hybridization advances to current year progress, it will help the progress for accelerating breeding improves the working efficiency of breeding.
Invention content
The purpose of the present invention is to provide the methods of STEVIA REBAUDIANA bi-annual planting breeding.
The invention is realized in this way：
A kind of bi-annual planting method of STEVIA REBAUDIANA breeding, includes the following steps：
(1) first makees seedling in greenhouse：Time early March, cuttage and seedling culture；
(2) first are colonized：Field field planting mid-April；
(3) first make economical character and quality determination：The first 15 days agronomy for carrying out STEVIA REBAUDIANA original material is harvested in first
The measurement of shape and quality is investigated;
(4) the second Hybridizing plan made is formulated：According to the researching determining for respectively hybridizing original material to STEVIA REBAUDIANA for the year and over the years
As a result, formulating the second Hybridizing plan made;
(5) second make cuttage and seedling culture：Stem section is cut on the plant that early July is made from first carries out cuttage and seedling culture, sunshade net covering
Shading；
(6) first make harvest time：Mid-July；
(7) second make potting：After second makees seedling incubation, it is colonized in flowerpot and cultivates；
(8) second make isolation cultivation：Before second blooms, each parents' pairing of the STEVIA REBAUDIANA for being ready for hybridization is put together,
And cultivation is isolated in the space that 500m or more is carried out with other STEVIA REBAUDIANA materials；At the florescence, the pollination that parents are carried out by insect hybridizes；
(9) second make Protectorate cultivation：Early October, weather turn cool, and potting STEVIA REBAUDIANA is moved into vinyl house, is protected
The cultivation of shield ground, ensures seed maturity；
(10) second make seed harvest：The seed for harvesting reciprocal cross after seed maturity respectively, for next work observation, compare.
It is characteristic of the invention that：
The crossbreeding of STEVIA REBAUDIANA is often to be hybridized using the breeding parent with different economical characters and quality trait, with
Make on the basis of parents to learn from other's strong points to offset one's weaknesses after phase hybridization, maximize favourable factors and minimize unfavourable ones, therefrom selects in yielding ability, insect resistace, disease-resistant
Property, Flood resistance, strong drought resistance, there is chrysanthemum Glycosides Contents height etc. breakthrough merit to combine.Go through the sight of work early period to parent
It is the basis for formulating next step breeding plan to examine, detect and compare.The growing process of usual the beginning of July, STEVIA REBAUDIANA have reached or
Close to the harvest time of STEVIA REBAUDIANA, various economical characters and qualitative characteristics are determined person, can rely on current year and successive dynasties pair early period
The observation of each parent material determines the Hybridizing plan of next work.The advantageous effect of the method for the present invention is：It is miscellaneous by previous 2 years
It hands over breeding work to focus on 1 year to carry out, reduces the period of STEVIA REBAUDIANA crossbreeding work, improve work efficiency；Due under
The stem section of interception STEVIA REBAUDIANA is used for cuttage on the STEVIA REBAUDIANA plant that half a year cultivation can directly be grown in the field, and eliminates spring cultivation
Training will occupy the expense of the occupied lagging facility of vinyl house nursery, thermal insulation material and management in winter.
The present invention has following difference compared with existing cultivation and breeding technique：
1. making full use of sunlight-heat reaources, first makees to make to intersect with second to carry out.
2. the first work and second is made all to utilize stem section cuttage, sowed without using seed, the saving Vegetative growth time, simultaneously
Utilize selection fine individual plant.
It is carried out 3. outdoor cropping and Protectorate cultivation intersect, ensures the temperature condition of growth of stevia rebaudiana.
Meaning of the present invention is to realize Temperate Region in China STEVIA REBAUDIANA breeding work bi-annual planting cultivation for the first time in the world, add
Fast Breeding progress；The operating costs for also saving breeding work simultaneously, have saved cost.The present invention has novelty and creation
Property, the method for the present invention is suitable for southern hemisphere and northern hemisphere mid latitudes, has practicability.
It is an advantage of the invention that：
1. reducing the STEVIA REBAUDIANA crossbreeding work period；Improve breeding efficiency.
2. saving breeding cost.
Specific implementation mode：
Below by specific implementation mode, the present invention will be further described.
32 degree of areas of north latitude, STEVIA REBAUDIANA parent material：F11-11 is selected and txb2.
Bi-annual planting cultural method, includes the following steps：
(1) first makees seedling in greenhouse：March 8 time, cuttage and seedling culture；
(2) first are colonized：April 15；
(3) first make economical character and quality determination：The first 15 days agronomy for carrying out STEVIA REBAUDIANA original material is harvested in first
The measurement of shape and quality is investigated.Field investigation and indoor detection show that F11-11 roguings system plant type is compact, and blade is thick and solid, plant
Property in great numbers is good, it is contemplated that single-strain blade yield can be relatively high, acquires its blade and carries out far infrared indoors and measures containing for its glucoside RA
Amount is 7.32%；And the plant growing way of txb2 is general, but the content of its RA is up to 8.8%.It is desirable that the hybridization for passing through both
Therefrom select that plant type is good, leaf yield is high, while the higher new varieties of RA contents.But from the point of view of the remaining season of growth, when
Year remaining time cannot meet next season growth of stevia rebaudiana and develop required sunlight-heat reaources, if plant again one season sweetleaf
Chrysanthemum, seed meeting can not be ripe.If according to the prior art, this work will postpone till coming year progress.And we are according to sweetleaf
The growth and development characteristic of chrysanthemum and facility condition at that time, having formulated a set of effective STEVIA REBAUDIANA parents current year measures, current year
Group pair, the technical solution of current year hybridization.
(4) second make cuttage and seedling culture：Stem section, which is cut, on the plant that July 9 made F11-11 choosings and txb2 from first carries out skewer
Insert nursery, sunshade net covering shading；
(5) on July 20 first made plant harvest；
(6) second make potting：Second makees August 5 days after seedling incubation, is colonized respectively in different flowerpots；
(7) second make isolation cultivation：Before second blooms, each parents' pairing of the STEVIA REBAUDIANA for being ready for hybridization is put together,
And cultivation is isolated in the space that 500m or more is carried out with other STEVIA REBAUDIANA materials；The pollination that florescence is carried out parents by insect hybridizes；
(8) second make Protectorate cultivation：Early October, weather turn cool, and potting STEVIA REBAUDIANA is moved into vinyl house, is protected
The cultivation of shield ground, ensures seed maturity；
(9) second make seed harvest：The seed of reciprocal cross is harvested after November 20 seed maturity respectively.
As a result of technical strategies, by natural conditions and freeze prevention measure reasonable link, implement STEVIA REBAUDIANA 1 year
Two make breeding method, carry the hybrid seed that the previous year obtains STEVIA REBAUDIANA parents than traditional method, improve the work of breeding
Efficiency.Meanwhile STEVIA REBAUDIANA parents seedling growing in summer and hybridization also save the required lagging facility of spring next year nursery, heat preservation
The expense of material and labor management.
Above-described embodiment is the preferred embodiment of the present invention, is not intended to limit the present invention, all spirit in the present invention and
In spirit, protection scope of the present invention is belonged to.
Claims (1)
1. a kind of bi-annual planting method of STEVIA REBAUDIANA breeding, steps are as follows：
(1) first makees seedling in greenhouse：Time early March, cuttage and seedling culture；
(2) first are colonized：Open country field planting mid-April；
(3) first make economical character and quality determination：The first 15 days agronomy for carrying out STEVIA REBAUDIANA original material is harvested in first
The measurement of shape and quality is investigated;
(4) the second Hybridizing plan made is formulated：According to the researching determining for respectively hybridizing original material to STEVIA REBAUDIANA for the year and over the years
As a result, formulating the second Hybridizing plan made;
(5) second make cuttage and seedling culture：Stem section is cut on the plant that early July is made from first carries out cuttage and seedling culture, sunshade net covering
Shading；
(6) first make harvest time：Mid-July；
(7) second make potting：After second makees seedling incubation, it is colonized in flowerpot and cultivates；
(8) second make isolation cultivation：Before second blooms, each parents' pairing of the STEVIA REBAUDIANA for being ready for hybridization is put together,
And cultivation is isolated in the space that 500m or more is carried out with other STEVIA REBAUDIANA materials；At the florescence, the pollination that parents are carried out by insect hybridizes；
(9) second make Protectorate cultivation：Early October, weather turn cool, and potting STEVIA REBAUDIANA is moved into vinyl house, is protected
The cultivation of shield ground, ensures seed maturity；
(10) second make seed harvest：The seed for harvesting reciprocal cross after seed maturity respectively, for next work observation, compare.
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| CN201810539597.5A CN108738776A (en) | 2018-05-30 | 2018-05-30 | A kind of bi-annual planting method of STEVIA REBAUDIANA breeding |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114667925A (en) * | 2022-04-15 | 2022-06-28 | 甘肃省农业工程技术研究院 | Northern area stevia rebaudiana hybrid combination matching and hybrid seed production method |
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|---|---|---|---|---|
| CN1555678A (en) * | 2003-12-31 | 2004-12-22 | 徐德芳 | Northern region paddy rice fast breeding technology |
| CN102487677A (en) * | 2011-11-18 | 2012-06-13 | 河南省农业科学院 | Production method for multiple cropping of corn, peanut or soybean in one year |
| CN103004450A (en) * | 2013-01-15 | 2013-04-03 | 山东农业大学 | Novel method of using land and greenhouse for anniversary cultivation of fourth-generation winter wheat |
| CN103749288A (en) * | 2014-02-24 | 2014-04-30 | 何克勤 | Stevia rebaudiana mesh enclosure hybrid method |
| CN105052503A (en) * | 2015-08-23 | 2015-11-18 | 林平 | Hybrid seed production method for stevia rebaudiana |
| CN106358630A (en) * | 2016-08-19 | 2017-02-01 | 江西省农业科学院作物研究所 | Brassica napus annual multi-generation generation adding method |
| CN106665306A (en) * | 2016-12-13 | 2017-05-17 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | Winter tobacco indoor generation adding breeding method |
| US20180070553A1 (en) * | 2014-09-02 | 2018-03-15 | Purecircle Sdn Bhd | Self-compatible stevia varieties, breeding methods therefrom and methods of preparing novel compositions using said varieties |
-
2018
- 2018-05-30 CN CN201810539597.5A patent/CN108738776A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1555678A (en) * | 2003-12-31 | 2004-12-22 | 徐德芳 | Northern region paddy rice fast breeding technology |
| CN102487677A (en) * | 2011-11-18 | 2012-06-13 | 河南省农业科学院 | Production method for multiple cropping of corn, peanut or soybean in one year |
| CN103004450A (en) * | 2013-01-15 | 2013-04-03 | 山东农业大学 | Novel method of using land and greenhouse for anniversary cultivation of fourth-generation winter wheat |
| CN103749288A (en) * | 2014-02-24 | 2014-04-30 | 何克勤 | Stevia rebaudiana mesh enclosure hybrid method |
| US20180070553A1 (en) * | 2014-09-02 | 2018-03-15 | Purecircle Sdn Bhd | Self-compatible stevia varieties, breeding methods therefrom and methods of preparing novel compositions using said varieties |
| CN105052503A (en) * | 2015-08-23 | 2015-11-18 | 林平 | Hybrid seed production method for stevia rebaudiana |
| CN106358630A (en) * | 2016-08-19 | 2017-02-01 | 江西省农业科学院作物研究所 | Brassica napus annual multi-generation generation adding method |
| CN106665306A (en) * | 2016-12-13 | 2017-05-17 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | Winter tobacco indoor generation adding breeding method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114667925A (en) * | 2022-04-15 | 2022-06-28 | 甘肃省农业工程技术研究院 | Northern area stevia rebaudiana hybrid combination matching and hybrid seed production method |
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Application publication date: 20181106 |