CN108721154A - A kind of skin care item and preparation method thereof for skin moisture-keeping and barrier reparation - Google Patents
A kind of skin care item and preparation method thereof for skin moisture-keeping and barrier reparation Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9711—Phaeophycota or Phaeophyta [brown algae], e.g. Fucus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The invention discloses a kind of skin care item for skin moisture-keeping and barrier reparation, the skin care item contain the component of following mass fraction：0.15%-0.25% marine fish skin collagen oligopeptides, 0.8%-1.2% Thallus Laminariae (Thallus Eckloniae) extracts, 0.04%-0.06% low molecular weight sodium hyaluronates.The invention also discloses the preparation methods of above-mentioned skin care item.There is skin care item of the present invention ideal moisturizing, nutrition repair, subject the symptoms such as rear face skin sensitivity, erythema are used continuously and are obviously improved it has been confirmed by experiments that, and impaired skin barrier is fast and effeciently repaired, and moisture content of skin significantly improves；All raw materials of skin care item of the present invention are natural extract or conventional skin care product additive, and the addition of each ingredient also complies with the custom requirements of skin care item production, is used for a long time safe and reliable.
Description
Technical field
The present invention relates to cosmetic technical fields, specifically, being a kind of skin care for skin moisture-keeping and barrier reparation
Product and its preparation method.
Background technology
The quality of skin moisture-keeping ability, can reflect skin barrier function it is normal whether.Skin moisture-keeping ability reduces
Skin barrier function is caused to decline, skin barrier function, which declines, further reduces skin moisture-keeping ability, causes vicious circle.Institute
It is the basis of skin nursing with moisturizing, and crucial, moisture-retention function of skin decline can lead to a variety of skin problems, it is skin care gold
One layer of the most bottom of word tower is the basis of skin care.When skin water shortage, cell metabolism and information transmission etc. are affected, or even go out
Existing obstacle, the physiological structure of skin change, such as dry, coarse, tarnish, and elasticity reduces, and wrinkle increases or deepens, color
Element increases, and top it all off water shortage causes skin barrier function obstacle, not only increases dermopathic neurological susceptibility, and can induce
With some skin diseases of exacerbation, such as dermatitis, eczema, psoriasis and acne.
Cuticula is the outermost layer of four layer tissues (basal layer, spinous layer, stratum granulosum, cuticula) of skin epidermis, by about 30 μ
M wide, the flat hexagonal horn cell composition of 0.8 μ m-thick, generally 10-20 confluent monolayer cells, thickness are 10-15 μm.Epidermis
Top layer also has lipid bilayer to form sebum film, it is the layer protecting film for being mixed by sweat and sebum and being formed.It anticipates from physiology
From justice, to prevent, Skin Cell internal moisture from losing cuticula, the erosion of external microbe and chemical substance provides extremely
Effective barrier action.
According to investigation, all to feel be " sensitive " by 60% or more Asia women and 50% European women.
The skin characteristic of this kind of crowd is：It is easy allergy, the apparent skin of red blood silk on the face is easy general red, is easy by bad border factor, season
Section variation and the stimulation of facial product etc..Most of document about sensitive skin concentrates on such a hypothesis, i.e., sensitive
Property skin Producing reason be due to stratum corneum barrier impaired or compound to the raising of Cutaneous permeation caused by.Skin as a result,
Skin barrier function damage has become the mechanism that the generally accepted sensitive of people generates.Repair the moisturizing shield of skin barrier
Skin product are generally configured with following 3 kinds of base stocks：(1) hygroscopic agent raw material includes glycerine, urea etc., and water can be absorbed from environment
Point, supplement distributes the moisture of loss from cuticula；(2) sealer raw material such as vaseline, silicone oil etc. can be formed thin in skin surface
Aqueous thin oil film plays the role of reinforcing skin barrier；(3) addition and epidermis, dermal components are same or analogous " bionical "
Raw material has the function of repairing skin barrier, such as ceramide, hyaluronic acid.These ingredients are mostly artificial synthesized change
Substance is learned, is used for a long time, potential injury is likely to result in skin.
Chinese patent literature CN107638331A discloses a kind of maintenance skin barrier and resists the moisturizing emulsion of free radical,
Including following weight percent composition：Polyalcohol 2%-6%, thickening suspending agent 0.2%-3%, natural saualane 1%-5%, breast
Agent 2.5%-4.5%, ceramide NP 0.1%-3%, niacinamide 0.1%-5%, Lycopene 0.1%-2%, grape pip carry
It is water to take object 0.5-3%, ferulic acid 0.1%-1%, Co-Q10 0.1%-2%, vitamin E 0.1-2%, surplus.It is Chinese special
Sharp document CN105748315B discloses a kind of composition for repairing skin barrier, and the composition is by weight percentage by following originals
Material is made：Sodium Hyaluronate 0.1%-1%, trehalose 0.5%-3%, moisturizer 5%-25% and water 71%-94.4%.China
Patent CN 108309920A disclose a kind of skin care item and preparation method thereof for repairing skin barrier, which repairs skin screen
The skin care item of barrier are by honeysuckle, wild chrysanthemum, menthol, borneol, vitamin E, propolis, Seabuckthorn Oil, glycerine, wormwood essential oil, wool
Fat, bisabolol, jojoba seed oil, Cer NP, Butyrospermum parkii fruit fat and Sodium Hyaluronate are made.But about the present invention
The active component of middle use yet there are no report in the application of skin barrier reparation.
Invention content
First purpose of the present invention is, for deficiency in the prior art, to provide a kind of for skin moisture-keeping and barrier
The skin care item of reparation.
Second object of the present invention is to provide a kind of preparation method of above-mentioned skin care item.
To realize above-mentioned first purpose, the technical solution adopted by the present invention is that：
A kind of skin care item for skin moisture-keeping and barrier reparation, the skin care item contain the group of following mass fraction
Point：0.15%-0.25% marine fish skin collagen oligopeptides, 0.8%-1.2% Thallus Laminariae (Thallus Eckloniae) extracts, 0.04%-0.06% low molecules
Measure Sodium Hyaluronate.
Preferably, the skin care item contain the component of following mass fraction：0.2% marine fish skin collagen oligopeptide,
1.0% Thallus Laminariae (Thallus Eckloniae) extract, 0.05% low molecular weight sodium hyaluronate.
Preferably, the skin care item are emulsifiable paste, are made of the raw material of following mass fraction：
It is highly preferred that above-mentioned emulsifiable paste is made of the raw material of following mass fraction：
Preferably, the skin care item are lotion, are made of the raw material of following mass fraction：
It is highly preferred that above-mentioned emulsion is made of the raw material of following mass fraction：
It is highly preferred that in above-mentioned skin care item, the marine fish skin collagen oligopeptide average molecular weight is less than 1000Da, low point
Son amount Sodium Hyaluronate average molecular weight is 3kDa-10kDa.
It is highly preferred that in above-mentioned skin care item, the preparation method of the Thallus Laminariae (Thallus Eckloniae) extract is：Kelp medicine materical crude slice is taken, is ground into thick
Powder adds 10~30 times of water, 70~90 DEG C of Extracting temperature, extraction time 8-12 hour to extract 3 times；Merge No. 3 extracting solutions, decompression
It is concentrated into and is equivalent to medicine materical crude slice 0.25g/ml；2~8 DEG C of low temperature is placed 12 hours, and precipitation is separated off, and supernatant is dried in vacuo, i.e.,
Obtain Thallus Laminariae (Thallus Eckloniae) extract.
It is highly preferred that in above-mentioned skin care item, the cream preparation method includes the following steps：
(1) each ingredient in addition to vitamin E in oil phase is taken, is heated to 70-80 DEG C, is melted；
(2) each in addition to low molecular weight sodium hyaluronate, carbomer, marine fish skin collagen oligopeptide, essence in phase of fetching water
Ingredient is added in appropriate purified water, is heated to 70-80 DEG C, dissolving；
(3) in advance that carbomer purifying is water-swellable, then it is added in (2)；
(4) (1) liquid is added in (3), is sufficiently stirred, vitamin E, low-molecular-weight hyaluronic acid are added when 40-50 DEG C
Sodium solution, marine fish skin collagen oligopeptide solution and essence, continue stirring until cold；
(5) dispense to get.
It is highly preferred that in above-mentioned skin care item, the emulsion preparation method includes the following steps：
(1) each ingredient in addition to vitamin E in oil phase is taken, is heated to 70-80 DEG C, is melted；
(2) except low molecular weight sodium hyaluronate, xanthans, carbomer, marine fish skin collagen oligopeptide, essence in phase of fetching water
Outer each ingredient, is added in appropriate purified water, is heated to nearly 70-80 DEG C, dissolving；
(3) in advance that carbomer, xanthans is water-swellable with purifying in right amount, then it is added in (2)；
(4) (1) liquid is added in (3), is sufficiently stirred, vitamin E, low-molecular-weight hyaluronic acid are added at nearly 40-50 DEG C
Sodium solution, marine fish skin collagen oligopeptide solution and essence, continue stirring until cold；
(5) dispense to get.
To realize above-mentioned second purpose, the technical solution adopted by the present invention is that：
Preparation method for skin moisture-keeping and the emulsifiable paste of barrier reparation as described above, includes the following steps：
(1) each ingredient in addition to vitamin E in oil phase is taken, is heated to 70-80 DEG C, is melted；
(2) each in addition to low molecular weight sodium hyaluronate, carbomer, marine fish skin collagen oligopeptide, essence in phase of fetching water
Ingredient is added in appropriate purified water, is heated to nearly 70-80 DEG C, dissolving；
(3) in advance that carbomer is water-swellable with purifying in right amount, then it is added in (2)；
(4) (1) liquid is added in (3), is sufficiently stirred, vitamin E, low-molecular-weight hyaluronic acid are added at nearly 40-50 DEG C
Sodium solution, marine fish skin collagen oligopeptide solution and essence, continue stirring until cold；
(5) dispense to get.
Preparation method for skin moisture-keeping and the lotion of barrier reparation as described above, includes the following steps：
(1) each ingredient in addition to vitamin E in oil phase is taken, is heated to 70-80 DEG C, is melted；
(2) except low molecular weight sodium hyaluronate, xanthans, carbomer, marine fish skin collagen oligopeptide, essence in phase of fetching water
Outer each ingredient, is added in appropriate purified water, is heated to nearly 70-80 DEG C, dissolving；
(3) in advance that carbomer, xanthans is water-swellable with purifying in right amount, then it is added in (2)；
(4) (1) liquid is added in (3), is sufficiently stirred, vitamin E, low-molecular-weight hyaluronic acid are added at nearly 40-50 DEG C
Sodium solution, marine fish skin collagen oligopeptide solution and essence, continue stirring until cold；
(5) dispense to get.
The active constituent of Thallus Laminariae (Thallus Eckloniae) extract includes fucoxanthine, laminarin, trehalose etc., has skin care moisturizing, antioxygen
The functions such as change, and can delay skin aging；Marine fish skin collagen oligopeptide (average molecular weight<1000Da) can effectively it be inhaled by skin
It receives, has that nutrition reparation, removing wrinkle and resisting aging be old, water lock moisturizing and other effects；Low molecular weight sodium hyaluronate (average molecular weight 3KDa~
It can 10KDa) be absorbed into inside skin and play better moisturizing and barrier repair function.
The invention has the advantages that：
1, zoopery proves, skin care item of the invention have apparent repairing effect to mouse damaged skin, are effectively increased
Epidermis, dermis thickness improve skin moisture content, reduce the loss of percutaneous moisture, have good barrier repairing effect.
2, it is verified by experiments, skin care item of the present invention have ideal moisturizing, trophism, after subject's continuous use
The symptoms such as skin of face sensitivity, erythema are obviously improved, and impaired skin barrier is fast and effeciently repaired, and moisture content of skin is bright
It is aobvious to improve.
3, all raw materials of skin care item of the present invention are natural extract or legal skin-care product additive, the addition of each ingredient
The custom requirements of skin care item production are also complied with, are used for a long time safe and reliable.
Description of the drawings
Fig. 1 is the measurement (single test) using transepidermal water content after product.
Fig. 2 is the measurement (follow-on test) using transepidermal water content after product.
Specific implementation mode
The invention will be further elucidated with reference to specific embodiments.It should be understood that these embodiments are merely to illustrate this hair
It is bright rather than limit the scope of the invention；In addition, it should also be understood that, after having read the content of the invention recorded, art technology
Personnel can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Fixed range.
Preparations of the embodiment 1-3 for skin moisture-keeping and the emulsifiable paste of barrier reparation
1 raw material
1 embodiment 1-3 of table is formulated with comparative example 1
2 preparation methods
The preparation of 2.1 Thallus Laminariae (Thallus Eckloniae) extracts
Kelp medicine materical crude slice is taken, coarse powder is ground into, 10 times of water, 70 DEG C of Extracting temperature, 12 hours extraction times is added to extract 3 times；It closes
And No. 3 extracting solutions, it is concentrated under reduced pressure into and is equivalent to medicine materical crude slice 0.25g/ml；2 DEG C of low temperature is placed 12 hours, and precipitation, supernatant are separated off
Liquid is dried in vacuo to get Thallus Laminariae (Thallus Eckloniae) extract.
The preparation of 2.2 emulsifiable pastes
(1) each ingredient in addition to vitamin E in oil phase is taken, is heated to 70-80 DEG C, is melted；
(2) each ingredient in phase of fetching water in addition to Sodium Hyaluronate, carbomer, marine fish skin collagen oligopeptide, essence, is added
In appropriate purified water, it is heated to nearly 70-80 DEG C, dissolving；
(3) in advance that carbomer is water-swellable with purifying in right amount, then it is added in (2)；
(4) (1) liquid is added in (3), is sufficiently stirred, vitamin E, sodium hyaluronate solution, sea are added at nearly 40-50 DEG C
Foreign fish skin collagen oligopeptide solution and essence, continue stirring until cold；
(5) dispense to get.
Preparations of the embodiment 4-6 for skin moisture-keeping and the lotion of barrier reparation
1 raw material
2 embodiment 4-5 of table is formulated with comparative example 2-4
2 preparation methods
The preparation of 2.1 Thallus Laminariae (Thallus Eckloniae) extracts
Kelp medicine materical crude slice is taken, coarse powder is ground into, 30 times of water, 90 DEG C of Extracting temperature, 8 hours extraction times is added to extract 3 times；It closes
And No. 3 extracting solutions, it is concentrated under reduced pressure into and is equivalent to medicine materical crude slice 0.25g/ml；2 DEG C of low temperature is placed 12 hours, and precipitation, supernatant are separated off
Liquid is dried in vacuo to get Thallus Laminariae (Thallus Eckloniae) extract.
The preparation of 2.2 lotions
(1) each ingredient in addition to vitamin E in oil phase is taken, is heated to 70-80 DEG C, is melted；
(2) except low molecular weight sodium hyaluronate, xanthans, carbomer, marine fish skin collagen oligopeptide, essence in phase of fetching water
Outer each ingredient, is added in appropriate purified water, is heated to nearly 70-80 DEG C, dissolving；
(3) in advance that carbomer, xanthans is water-swellable with purifying in right amount, then it is added in (2)；
(4) (1) liquid is added in (3), is sufficiently stirred, vitamin E, low-molecular-weight hyaluronic acid are added at nearly 40-50 DEG C
Sodium solution, marine fish skin collagen oligopeptide solution and essence, continue stirring until cold；
(5) dispense to get.
The skin irritation of 7 lotion of embodiment is evaluated
New Zealand White Rabbit 8, experiment is selected to cut within first 1 day the top diamond wool in animal subject back, left and right each about 3cm
× 3cm, during which cannot damage epidermis, otherwise select spare rabbit.Take skin care item prepared by 0.5mL embodiments 1 and embodiment 4 straight
It connects coated on the skin of right side, prevents rabbit from shaking and losing tested material, with two layers of gauze and one layer of plastic sheet covering, then use
Bandage is fixed, using left side skin as blank control.Using blocking test, the application time is 4 hours.After the test, clear water is used
Tested area is rinsed, and dermoreaction situation was observed in 1,24,48 and 72 hour, according to《Cosmetics health specification (2007 editions)》In
Skin irritation test carries out dermoreaction scoring, as a result shows that the integral mean value of each experimental animal skin wound repair is 0, does not draw
The skin adverse reactions such as erythema or oedema are played, show that the skin care item of the present invention have cutaneous safety.
The moisture retention of 8 emulsifiable paste of embodiment is evaluated
1 test crowd
Tested number：15 people；Age：20 years old~40 years old；Gender：10 people of female, male 5 people；Health status：Subjects skin is strong
Health, no skin disease and allergies.
Experimental group：Moisturizing emulsifiable paste obtained in embodiment 1；
Control group：Base cream obtained in comparative example 1.
2 experimentations
Test environment temperature is 25 DEG C, and relative humidity (RH) is 61%, and 10min exposes tested position before measuring, simultaneously
Examinee is allowed to quiet down in the environment.The square Experimental Area of 3.5cm × 3.5cm is respectively marked on the forearm of left and right, and
Experimental Area is numbered, left arm smears the moisturizing emulsifiable paste of experimental group, and the symmetrical region of right arm smears the moisturizing emulsifiable paste of control group.It is first
Recipient site first is cleaned using alkaline perfumed soap, is dried after rinsing well.Before makeup application, Corneometer CM825 are used
Tester measures the cuticula moisture for being tested position.It takes 0.1g experimental products to be applied to recipient site, uniformly applies on the skin
Smear 2min.In 10min, 40min, 60min, 120min and 180min using 825 testers of Corneometer CM respectively into
Row is single and continuous measurement, tests out keratoderma moisture content value (its linear module with a.u. to indicate), and record
Test result.It chooses 10 points in tested region to measure, and it is single test (Fig. 1) to record mean values.Exist simultaneously
Intermediate point is chosen in tested region and measures 70s, and it is follow-on test (Fig. 2) to record mean values.
3 experimental results
It is apparently higher than control group using the transepidermal water content of experimental group moisturizing emulsifiable paste, difference has conspicuousness (P>
0.05)。
The skin barrier repair of 9 lotion of embodiment is evaluated
1 test crowd
Sensitive, 30, man 1, female 29, average age 28.4 (19~50) year, average course of disease 20 months.
Inclusion criteria：(1) meet sensitive criterion, i.e. skin of face after environmental stimuli by being susceptible to scabies
Itch, be scorching hot, shouting pain, the subjective sensations such as tight, companion or not with the objective signs such as erythema, furfur, lactic acid prick test >=3 point, nothing
There is small degree change in wound skin detection skin；(2) subject during observation without using other antiallergies are oral and external application
Drug；(3) physician guidance use is pressed during research by test product.
2 experimental methods
Moisturizing emulsion after daily face cleaning at the cheek in external application embodiment 4, each 1 time sooner or later, continuously with 28 days.Observation period
Between do not use other similar lotions and drug, respectively after on-test the 0th and 28 day assessment curative effect.Evaluation index includes breast
It is sour prick test scoring (scoring of abbreviation lactic acid), water content of stratum corneum, absolute through epidermis fluid loss (TEWL) and VISIA erythema
Value.
Lactic acid prick test：At room temperature, 10% lactic acid solution, 50 μ l are applied to right side nasolabial groove and arbitrary one side
Cheek, inquires the subjective symptoms of subject in 2.5min and 5min respectively, scored by 4 point-scores (1 point is no tingling sensation, 2
It is divided into slight shouting pain, 3 points are moderate shouting pain, and 4 points are severe shouting pain), then 2 scores are added, total score >=3 point are pierced for lactic acid
Pain experiment reacting positive.
Water content of stratum corneum and TEWL in 20~25 DEG C of room temperature, the indoor measurement that relative air humidity is 50%~60%,
Rest 15min after subject washes one's face with warm water before measuring takes same position to measure every time, is measured 3 times per position and takes mean value.
45 ° of loxosis photos of subject's face normotopia and bilateral are shot using VISIA skin detection instrument, record bilateral face is red
Spot absolute value.
3 experimental results
Lactic acid scoring significantly reduces (P before relatively treating<0.01), wherein 11<3 points.Specifically score value is：0th day (4 ± 2)
Point, the 28th day (3 ± 2) point.
Water content of stratum corneum dramatically increases (P before relatively treating after 28 days<0.01), TEWL significantly reduces (P<0.01).Specifically
Water content of stratum corneum is：0th day is (45.5 ± 16.3) %, and the 28th day is (57.1 ± 12.5) %.TEWL is：It is within 0th day
(20.7±11.2)g·cm-2·h-1, the 28th day is (12.9 ± 5.9) gcm-2·h-1。
Bilateral face red spot absolute value significantly improves (P before relatively treating after 28 days<0.05).Specifically erythema absolute value is：It is left
Cheek, the 0th day is (42.2 ± 10.3), and the 28th day is (37.5 ± 8.5)；Right side cheek, the 0th day be (40.9 ± 10.5), the 28th
It is (37.1 ± 8.0).
The result shows that there is apparent recovery skin barrier function using moisturizing emulsion.
Embodiment 10 repairs the experiment of skin barrier Contrast on effect
1 experimentation
The reparation of 1.1 pairs of adhesive tape tearing model mices
Healthy female kunming mice 25,25 ± 2g of weight is taken, mouse is divided into 5 groups, respectively blank group, comparative example 2
Group, 3 groups of comparative example, 4 groups of comparative example and experimental group.Mouse back loses hair or feathers, and is cleaned out with physiological saline, viscous with mightiness belt
Skin 4-5 times at this is pasted, 2 times a day, from continuous 2 days, the 3rd day, in mouse damaged part, blank group is not dealt with, experimental group
Lotion made from embodiment 4 is smeared, remaining each group smears corresponding experiment product, 2 times a day.Continuous 7 days, detection was through the severe edema due to hypofunction of the spleen within the 8th day
Divide and loses (TEWL).
The reparation of 1.2 pairs of ultraviolet ageing model mices
Healthy female kunming mice 25,25 ± 2g of weight is taken, mouse is divided into 5 groups, respectively blank group, comparative example 2
Group, 3 groups of comparative example, 4 groups of comparative example and experimental group.Mouse back loses hair or feathers, and is cleaned out with physiological saline, ultraviolet with 400nm
Line irradiates, and 2 times a day, continues 15 days.From 16th day, in mouse damaged part, blank group is not dealt with, and experimental group, which is smeared, to be implemented
Lotion made from example 4, remaining each group smear corresponding experiment product, 2 times a day.Mouse is put to death after continuous 15 days.Inspection mouse skin,
Dermis thickness and skin moisture content.
Percutaneous loss of moist (TEWL) usesEvaporimeter(Cortex Technology,
Denmark it) measures.Mouse skin, dermis thickness are measured to be measured using under 5 non-overlapping visuals field of skin paraffin section under microscope
Average value determines.Skin moisture content dries the difference determination of dry weight after 12h with 1 × 1cm dermatological specimens weight in wet base with 80 DEG C of baking ovens.
2 experimental results
The reparation of 2.1 pairs of adhesive tape tearing model mouse skins
Compared with blank group, comparative example 2,3,4 groups and experimental group TEWL amounts are substantially reduced, and difference has statistical significance (P
<0.05).Without significant difference between comparative example 2,3,4 groups；Compared with comparative example 2,3,4, experimental group is substantially reduced TEWL amounts, poor
It is different that there is statistical significance (P<0.05).
| Group | Percutaneous moisture (TEWL)/gm-2·h-1 |
| Blank group | 37.1±8.1 |
| 2 groups of comparative example | 29.4±4.5 |
| 3 groups of comparative example | 31.7±4.1 |
| 4 groups of comparative example | 28.4±3.6 |
| Experimental group | 21.1±3.5 |
The reparation of 2.2 pairs of ultraviolet ageing model mices
Compared with blank group, comparative example 2,3,4 groups and experimental group epidermis, dermis thickness and moisture content of skin significantly improve,
Difference has statistical significance (P<0.05), without significant difference between comparative example 2,3,4 groups, compared with comparative example 2,3,4, experiment
The apparent epidermis of group, dermis thickness and moisture content of skin obviously increase, and difference has statistical significance (P<0.05).
Above-mentioned the results show, skin care item of the invention have apparent repairing effect to mouse damaged skin, effectively increase
Add epidermis, dermis thickness, improve skin moisture content, reduce the loss of percutaneous moisture, there is good barrier repairing effect.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, under the premise of not departing from the method for the present invention, can also make several improvement and supplement, these are improved and supplement also should be regarded as
Protection scope of the present invention.
Claims (10)
1. a kind of skin care item for skin moisture-keeping and barrier reparation, which is characterized in that skin care item contain following mass fraction
Component：0.15%-0.25% marine fish skin collagen oligopeptides, 0.8%-1.2% Thallus Laminariae (Thallus Eckloniae) extracts, low point of 0.04%-0.06%
Son amount Sodium Hyaluronate.
2. according to claim 1 be used for skin moisture-keeping and barrier reparation skin care item, which is characterized in that skin care item contain as
The component of lower mass fraction：0.2% marine fish skin collagen oligopeptide, 1.0% Thallus Laminariae (Thallus Eckloniae) extract, 0.05% low molecular weight hyalomitome
Sour sodium.
3. being used for the skin care item of skin moisture-keeping and barrier reparation according to claim 1, which is characterized in that skin care item are breast
Cream is made of the raw material of following mass fraction：
4. being used for the skin care item of skin moisture-keeping and barrier reparation according to claim 3, which is characterized in that the emulsifiable paste is by such as
The raw material of lower mass fraction forms：
5. being used for the skin care item of skin moisture-keeping and barrier reparation according to claim 1, which is characterized in that skin care item are breast
Liquid is made of the raw material of following mass fraction：
6. being used for the skin care item of skin moisture-keeping and barrier reparation according to claim 5, which is characterized in that the lotion is by such as
The raw material of lower mass fraction forms：
7. according to any skin care item for being used for skin moisture-keeping and barrier reparation of claim 1-6, which is characterized in that the sea
Foreign fish skin collagen oligopeptide average molecular weight is less than 1000Da, and low molecular weight sodium hyaluronate average molecular weight is 3kDa-
10kDa。
8. according to any skin care item for being used for skin moisture-keeping and barrier reparation of claim 1-6, which is characterized in that the sea
Preparation method with extract is：Kelp medicine materical crude slice is taken, coarse powder is ground into, adds 10~30 times of water, 70~90 DEG C of Extracting temperature, extraction
Time 8-12 hour extracts 3 times；Merge No. 3 extracting solutions, is concentrated under reduced pressure into and is equivalent to medicine materical crude slice 0.25g/ml；2~8 DEG C of low temperature is put
It sets 12 hours, is separated off precipitation, supernatant is dried in vacuo to get Thallus Laminariae (Thallus Eckloniae) extract.
9. according to any skin care item for being used for skin moisture-keeping and barrier reparation of claim 3 or 4, which is characterized in that described
Cream preparation method includes the following steps：
(1) each ingredient in addition to vitamin E in oil phase is taken, is heated to 70-80 DEG C, is melted；
(2) each ingredient in phase of fetching water in addition to low molecular weight sodium hyaluronate, carbomer, marine fish skin collagen oligopeptide, essence,
It is added in appropriate purified water, is heated to 70-80 DEG C, dissolving；
(3) in advance that carbomer purifying is water-swellable, then it is added in (2)；
(4) (1) liquid is added in (3), is sufficiently stirred, it is molten that vitamin E, low molecular weight sodium hyaluronate are added when 40-50 DEG C
Liquid, marine fish skin collagen oligopeptide solution and essence, continue stirring until cold；
(5) dispense to get.
10. any skin care item for being used for skin moisture-keeping and barrier reparation of claim 5 or 6, which is characterized in that the lotion
Preparation method includes the following steps：
(1) each ingredient in addition to vitamin E in oil phase is taken, is heated to 70-80 DEG C, is melted；
(2) in phase of fetching water in addition to low molecular weight sodium hyaluronate, xanthans, carbomer, marine fish skin collagen oligopeptide, essence
Each ingredient is added in appropriate purified water, is heated to 70-80 DEG C, dissolving；
(3) in advance that carbomer, xanthans is water-swellable with purifying in right amount, then it is added in (2)；
(4) (1) liquid is added in (3), is sufficiently stirred, be added at 40-50 DEG C vitamin E, low molecular weight sodium hyaluronate solution,
Marine fish skin collagen oligopeptide solution and essence continue stirring until cold；
(5) dispense to get.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811033414.9A CN108721154B (en) | 2018-09-05 | 2018-09-05 | Skin care product for skin moisturizing and barrier repair and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811033414.9A CN108721154B (en) | 2018-09-05 | 2018-09-05 | Skin care product for skin moisturizing and barrier repair and preparation method thereof |
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| CN108721154B CN108721154B (en) | 2021-03-30 |
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| Application Number | Title | Priority Date | Filing Date |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109793672A (en) * | 2018-12-24 | 2019-05-24 | 中健(广州)生物医药研究院有限公司 | Application of the fish oligopeptide in cosmetics |
| CN111214418A (en) * | 2020-03-13 | 2020-06-02 | 佛山市奥姿美生物科技有限公司 | Efficient barrier repair composition and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104042541A (en) * | 2014-05-31 | 2014-09-17 | 青岛博泓海洋生物技术有限公司 | Mild moisturizing lotion |
| CN106963697A (en) * | 2017-05-19 | 2017-07-21 | 广州蜜妆生物科技有限公司 | It is a kind of that there is oil-control, the facial mask liquid of pore refining effect |
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2018
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| CN104042541A (en) * | 2014-05-31 | 2014-09-17 | 青岛博泓海洋生物技术有限公司 | Mild moisturizing lotion |
| CN106963697A (en) * | 2017-05-19 | 2017-07-21 | 广州蜜妆生物科技有限公司 | It is a kind of that there is oil-control, the facial mask liquid of pore refining effect |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109793672A (en) * | 2018-12-24 | 2019-05-24 | 中健(广州)生物医药研究院有限公司 | Application of the fish oligopeptide in cosmetics |
| CN111214418A (en) * | 2020-03-13 | 2020-06-02 | 佛山市奥姿美生物科技有限公司 | Efficient barrier repair composition and application thereof |
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| Publication number | Publication date |
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| CN108721154B (en) | 2021-03-30 |
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