CN108714189B - Liver-protecting intestinal microecological regulator based on liver-intestinal axis and preparation method thereof - Google Patents
Liver-protecting intestinal microecological regulator based on liver-intestinal axis and preparation method thereof Download PDFInfo
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/64—Orobanchaceae (Broom-rape family)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention relates to a liver-protecting intestinal microecological regulator based on a liver-intestinal axis and a preparation method thereof, wherein the regulator is prepared from the following raw materials in parts by weight: 100-300 parts of humifuse euphorbia herb, 60-210 parts of Ampelopsis grossedentata leaf, 100-300 parts of raspberry, 100-300 parts of purslane, 60-180 parts of rhizoma polygonati, 50-150 parts of cistanche, 5-20 parts of yeast beta-glucan, 10-50 parts of fructo-oligosaccharide and 10-60 parts of soybean oligosaccharide. Compared with the single use of medicines or prebiotics, the composition has the advantages that the curative effect is remarkably improved, the composition takes the health-care theory of the traditional Chinese medicine and the liver-intestine axis theory of the modern medical research as guidance, the components are matched with each other, the synergy is realized, the composition is safe and has no toxic or side effect, the tolerance and the drug resistance can not be generated, the accumulation in the body can not be realized, and the functions of protecting the liver and regulating the intestinal microecology are definite; the preparation method of the invention not only can furthest reserve each main active component, improve the yield of the extract, but also can effectively reduce the extraction time and reduce the energy consumption.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a liver-protecting intestinal microecological regulator based on a liver-intestinal axis and a preparation method thereof.
Background
The liver is an organ with the main functions of metabolism and detoxification, while the intestinal tract is an organ with the main functions of digestion and absorption of nutrients, which are generally considered to be independent and not interfered with each other. With the development of science and technology and the continuous and deep research, more and more evidences indicate that the liver and the intestinal tract are closely related and mutually influenced in biological function, and the theory of the "liver-intestine axis" is born. In the embryonic stage, the liver and intestinal tract commonly originate in the foregut, and after maturation the two organs are mainly associated with each other by the portal vein: approximately 70% of the blood supply in the liver comes from the portal vein, while the return of venous blood from the gut is the main source of portal blood, and many of the nutrients and toxins absorbed through the gut need to be metabolized by the liver. The human intestinal tract inhabits a large number of microorganisms, and the microorganisms live and evolve together with human beings to the present, so that a stable intestinal tract micro-ecological system is gradually formed, and the intestinal tract micro-ecological system has the physiological functions of maintaining the integrity of intestinal epithelial cells and intestinal mucosa barriers, promoting the nutrient absorption and intestinal tract peristalsis of human bodies, synthesizing various vitamins, inhibiting the growth of pathogenic flora, regulating immunity and the like, and is an indispensable element for human health. In recent years, the relationship between intestinal micro-ecology and health and diseases has increasingly become a focus and a hot spot in the field of global life science research.
The liver can not only remove various toxins from the intestinal tract and enterogenous microbes, but also regulate the structural function of intestinal flora through bile acid secretion and intestinal liver circulation, maintain the intestinal microecological balance, and the intestinal flora can convert primary bile acid discharged into the intestinal tract by the liver into secondary bile acid which is then reabsorbed by the intestinal wall, so that the obtained recycled bile acid can stimulate bile secretion, can dredge the biliary tract, eliminate harmful substances such as mycotoxin and bacterial endotoxin, and play a role in protecting the liver and the gallbladder. A number of clinical studies have shown that intestinal micro-ecology plays a crucial role in the pathophysiological processes of the liver, which in turn affects the structure and function of intestinal micro-ecology when suffering from liver diseases. Normally, only trace amounts of intestinal microorganisms can pass through the intestinal mucosal barrier and reach the liver via the portal vein, but instead can activate the reticuloendothelial system of the liver, so that the reticuloendothelial system is continuously in an activated state. When the intestinal microecological balance is disordered, the intestinal mucosa barrier can be damaged, a large amount of intestinal microorganisms and toxins can be shifted to the liver, the immune tolerance of the liver is damaged, the inherent immune system of the liver is excessively activated to generate inflammatory cascade reaction, bacteremia and endotoxemia are caused, and the liver injury is induced. When the liver is damaged and has pathological changes, the synthesis function of the liver is reduced, the albumin in the blood plasma is rapidly reduced, and the abdominal cavity hydrops is easily caused; meanwhile, the liver secretes insufficient bile, so that bile salt is lacked in the intestinal tract, portal hypertension is formed, and the gastrointestinal tract is extravasated with blood and is anoxic, so that portal hypertensive gastroenteropathy occurs; the impaired liver has a reduced synthesis of coagulation factors, which aggravates gastrointestinal bleeding symptoms and further worsens intestinal microecological balance. Therefore, the intestinal microecology is closely related to the liver, and the intestinal dysbiosis and the liver disease are causal, so that a series of pathological changes of the organism are caused. The close relationship between the two also suggests that attention should be paid to the regulation of intestinal micro-ecology when liver diseases occur, and to the protection of the liver when intestinal micro-ecology is disturbed. Provides a brand new idea for researching and preventing intestinal microecological imbalance and pathogenesis of liver diseases.
With the rapid development of society, the pace of life is faster and faster, the dietary structure and the living habits of people are greatly changed, and the liver and intestinal micro-ecology of people is heavily burdened by long-term staying up all night, irregular diet, smoking, large amount of drinking, unreasonable medication and the like. However, most of the existing products in the market are distinguished from each other: various liver diseases are treated by commonly using liver protection tablets, bifendate, compound liver benefiting medicine and the like so as to achieve the purposes of nourishing and protecting the liver, but the problems of slow effect, long taking period and low bioavailability of the medicine exist, and even adverse reactions such as nausea, vomiting, dermatitis, rash and the like can be caused; probiotics, prebiotics or synbiotics and the like are frequently used for conditioning intestinal micro-ecology, the intestinal regulator, particularly the regulator containing probiotics, has the problems of single and unstable bacterial strains, difficult storage, low survival rate of the bacterial strains, low colonization rate and the like, and has the phenomena of low cure rate and easy relapse for many people with liver diseases. Therefore, it is an important subject to be solved at present to correctly recognize the relationship between the liver and the intestinal microecology and to develop a liver-protecting intestinal microecology regulator with safety, high efficiency, small toxic and side effects, and stable and controllable quality based on the relationship.
Disclosure of Invention
The invention aims to solve the technical problem of the prior art and provides a liver-protecting intestinal microecological regulator based on a liver-intestinal axis, which is safe and efficient, has small toxic and side effects, stable and controllable quality, does not generate tolerance and drug resistance, does not accumulate in vivo, and has the functions of protecting the liver and regulating intestinal microecological functions.
The invention aims to solve another technical problem and provides a preparation method of the liver-protecting intestinal microecological regulator based on the liver-intestinal axis, aiming at the current situation of the prior art, wherein the preparation method can not only reserve each main active component to the maximum extent and improve the extract yield, but also can effectively reduce the extraction time and reduce the energy consumption.
The technical scheme adopted by the invention for solving the technical problems is as follows: a liver-protecting intestinal microecological regulator based on a liver-intestinal axis is characterized in that: the preparation raw materials of the regulator comprise the following components in percentage by weight
100-300 parts of humifuse euphorbia herb, 60-210 parts of Ampelopsis grossedentata leaf, 100-300 parts of raspberry, 100-300 parts of purslane, 60-180 parts of rhizoma polygonati, 50-150 parts of cistanche, 5-20 parts of yeast beta-glucan, 10-50 parts of fructo-oligosaccharide and 10-60 parts of soybean oligosaccharide.
Preferably, the regulator is prepared from the following raw materials in parts by weight: 210 parts of humifuse euphorbia herb, 165 parts of ampelopsis grossedentata leaves, 240 parts of raspberry, 210 parts of purslane, 140 parts of rhizoma polygonati, 100 parts of cistanche, 16 parts of yeast beta-glucan, 24 parts of fructo-oligosaccharide and 30 parts of soybean oligosaccharide.
Preferably, the regulator comprises humifuse euphorbia herb extract, ampelopsis grossedentata leaf extract, raspberry extract, purslane extract, sealwort extract, cistanche deserticola extract, yeast beta-glucan, fructo-oligosaccharide and soybean oligosaccharide. The regulator of the invention takes the components as main active substances, and can be prepared into any preparation form suitable for oral administration, such as tablets, capsules, granules, powder, pills or oral liquid, by adopting the conventional technology of pharmaceutical science by preferably adding proper auxiliary materials.
As an improvement, the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract and the raspberry extract are obtained by post-treatment through a flash extraction and vacuum decompression method, and the purslane extract, the sealwort extract and the cistanche extract are obtained by post-treatment through a mechanochemical and ultrasonic wave auxiliary method. The flash extraction and vacuum decompression method and the mechanochemical synergistic ultrasonic auxiliary method are adopted to extract different types of components, so that the main active components can be retained to the maximum extent, the extract yield is improved, the extraction time can be effectively shortened, and the energy consumption is reduced.
The preparation method of the liver-protecting intestinal microecological regulator based on the liver-intestinal axis is characterized by comprising the following steps of:
(1) respectively preparing herba Euphorbiae Humifusae extract, Ampelopsis grossedentata leaf extract, and Rubi fructus extract;
(2) respectively preparing purslane extract, rhizoma polygonati extract and cistanche extract;
(3) mixing the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract, the raspberry extract, the purslane extract, the polygonatum extract and the cistanche extract prepared in the step (1) and the step (2), adding yeast beta-glucan, fructo-oligosaccharide and soybean oligosaccharide which are in corresponding weight components, and uniformly mixing to obtain the liver-protecting intestinal microecological regulator based on the liver-intestinal axis.
Preferably, the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract and the raspberry extract are obtained through the following steps:
(A) flash extraction: adding the ground humifuse euphorbia herb/Ampelopsis grossedentata leaf/raspberry which are sieved by a sieve of 10-80 meshes into an extraction tank, adding 8-30 times of 20-75% ethanol serving as an extraction solvent, and extracting at room temperature for 5-120 s;
(B) vacuum decompression extraction: opening a communication valve between the flash extractor and the vacuum reduced pressure extractor, and allowing the feed liquid to enter the vacuum reduced pressure extractor under the action of pressure difference for extraction until an extracting solution is obtained;
(C) centrifugal separation: centrifuging the obtained extractive solution to obtain clarified solution;
(D) and (3) concentrating under reduced pressure: concentrating the clarified solution under reduced pressure to obtain extract;
(E) spray drying: spray drying the extract to obtain herba Euphorbiae Humifusae extract/Ampelopsis grossedentata leaf extract/Rubi fructus extract.
Preferably, the extraction temperature of the vacuum reduced pressure extraction is 40-65 ℃, the extraction pressure is 0.05-0.1 MPa, and the extraction time is 20-40 min; the concentration temperature of the reduced pressure concentration is 50 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15.
Preferably, the spray drying process parameters are that the nozzle temperature is 160-180 ℃, the outlet temperature is 70-85 ℃, and the feeding speed is 10-20 mL/min.
Preferably, the purslane extract, the sealwort extract and the cistanche extract are obtained by the following steps:
(A) mechanical chemical extraction: adding the coarse purslane/rhizoma polygonati/cistanche salsa into a ball milling tank, and adding Na with the volume concentration of 5-30%2CO3As grinding auxiliary agent, the extraction time is 3 min-15 min, and the condensed water is started in the whole process;
(B) ultrasonic-assisted extraction: transferring the material obtained by mechanochemical extraction into an ultrasonic extractor, and adding 10-30 times of water as an extraction solvent to obtain an extracting solution;
(C) centrifugal separation: centrifuging the obtained extractive solution to obtain clarified solution;
(D) and (3) concentrating under reduced pressure: concentrating the clear liquid under reduced pressure, wherein the concentration temperature is 60 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract;
(E) spray drying: and (3) spray drying the extract, wherein the process parameters are that the nozzle temperature is 130-160 ℃, the outlet temperature is 60-80 ℃, and the feeding speed is 10-20 mL/min, so that the purslane extract/the rhizoma polygonati extract/the cistanche extract is obtained.
Preferably, the extraction temperature of the ultrasonic-assisted extraction is 40-80 ℃, the extraction time is 20-80 min, and the ultrasonic power is 100-.
Compared with the prior art, the invention has the advantages that:
in the formula of the regulator, multiple natural products are scientifically and reasonably matched, and are synergistic, so that the regulator can protect the liver and promote intestinal microecological regulation. The humifuse euphorbia herb and the purslane enter liver and large intestine channels together for matching use, cooling blood and removing toxicity, treating diarrhea and dysentery simultaneously, particularly having stronger inhibiting effect on various intestinal pathogenic bacteria such as escherichia coli, dysentery bacillus, salmonella, shigella, staphylococcus aureus and the like, and being matched with the Ampelopsis grossedentata to further strengthen the inhibiting effect on the pathogenic bacteria such as the escherichia coli, the staphylococcus aureus and the like, while intestinal probiotic activated proliferation factors such as yeast beta-glucan, fructo-oligosaccharide, soybean oligosaccharide and the like can effectively promote the proliferation of probiotics, and can effectively inhibit the generation and the excretion of intestinal putrefactive substances, the raw materials are organically combined, and the balance of intestinal microecology can be effectively maintained; meanwhile, the combination of the humifuse euphorbia herb, the ampelopsis grossedentata leaf and the purslane can play a role in protecting the liver by increasing the toxin expelling capability of the liver, while the raspberry can enhance the activity of antioxidant enzymes of liver tissues and inhibit the generation of inflammatory factors, and the cistanche deserticola has stronger antioxidant function and reduces the generation of free radicals, thereby reducing the damage of the free radicals to the liver tissues and mitochondria, further improving the oxidation resistance of the liver, reducing lipid peroxidation, and the two have synergistic interaction with the sealwort, can obviously enhance the capability of organism in removing free radicals and lipid peroxides in cells, and the yeast beta-glucan has a matching effect with the yeast beta-glucan, can promote immune cells to generate specific combination, stimulate lymphocytes, macrophages and the like in vivo to generate immune activity, effectively improve the immunity of organisms, promote the recovery of damaged tissues and organs such as livers and the like, and further improve the intestinal microecological balance through a liver-intestinal axis. Therefore, the invention organically combines a plurality of natural products for use, promotes the discharge of liver toxin, repairs damaged liver tissues and protects the liver under the synergistic action on the microbial level, the cell level and the metabolic level, and can effectively regulate the micro-ecological balance of the intestinal tract of an organism and promote the health of the intestinal tract.
Compared with the single use of medicaments or the single use of prebiotics, the composition has obviously improved curative effect, is provided under the guidance of the health-care theory of the traditional Chinese medicine and the liver-intestine axis theory of the modern medical research, and has the advantages that the components are mutually matched and synergized, and not only are the single substances superposed;
the liver-protecting intestinal microecological regulator mainly takes natural traditional Chinese medicines and prebiotics as raw materials, is basically homologous in medicine and food or a new food raw material, is safe, has no toxic or side effect, cannot generate tolerance and drug resistance, cannot accumulate in vivo, and has exact functions of protecting liver and regulating intestinal microecological;
in the preparation method, the flash extraction is cooperated with the vacuum decompression method and the mechanochemical cooperation ultrasonic wave auxiliary method are adopted to extract different types of components, so that not only can main active components be retained to the maximum extent, the extract yield is improved, but also the extraction time can be effectively reduced, and the energy consumption is reduced.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1:
the liver-protecting intestinal micro-ecological regulator based on the liver-intestinal axis of the embodiment is prepared by adopting the following raw materials by weight: 150 parts of humifuse euphorbia herb, 130 parts of ampelopsis grossedentata leaves, 200 parts of raspberry, 150 parts of purslane, 120 parts of rhizoma polygonati, 60 parts of cistanche, 8 parts of yeast beta-glucan, 15 parts of fructo-oligosaccharide and 20 parts of soybean oligosaccharide.
The preparation method of the liver-protecting intestinal microecological regulator based on the liver-intestinal axis comprises the following steps:
(1) respectively preparing herba Euphorbiae Humifusae extract, Ampelopsis grossedentata leaf extract, and Rubi fructus extract;
adding pulverized herba Euphorbiae Humifusae/Ampelopsis grossedentata leaf/Rubi fructus sieved with 10 mesh sieve into an extraction tank, adding 10 times of 30% ethanol as extraction solvent, and extracting at room temperature for 10 s; opening a communicating valve between the flash extractor and the vacuum reduced pressure extractor, allowing the feed liquid to enter the vacuum reduced pressure extractor under the action of pressure difference, extracting at 40 deg.C under 0.05MPa for 20min to obtain extractive solution; carrying out centrifugal separation on the obtained extracting solution, and then carrying out reduced pressure concentration, wherein the concentration temperature is 50 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract; spray drying the extract at a nozzle temperature of 160 ℃, an outlet temperature of 70 ℃ and a feeding speed of 10mL/min to obtain a humifuse euphorbia herb extract, a ampelopsis grossedentata leaf extract or a raspberry extract;
(2) respectively preparing purslane extract, rhizoma polygonati extract and cistanche extract;
adding coarse powder of herba Portulacae/rhizoma Polygonati/Cistanchis herba into ball milling tank, adding 5% Na2CO3As grinding auxiliary agent, extracting for 3min, and starting condensed water in the whole process; transferring the material obtained by mechanochemical extraction into an ultrasonic extractor, adding 10 times of water as an extraction solvent, wherein the extraction temperature is 40 ℃, the extraction time is 20min, and the ultrasonic power is 150W to obtain an extracting solution; carrying out centrifugal separation on the obtained extracting solution, and then carrying out reduced pressure concentration, wherein the concentration temperature is 60 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract; spray drying the extract at a nozzle temperature of 130 ℃, an outlet temperature of 60 ℃ and a feeding speed of 10mL/min to obtain a purslane extract or a rhizoma polygonati extract or a cistanche extract;
(3) mixing the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract, the raspberry extract, the purslane extract, the polygonatum extract and the cistanche extract prepared in the step (1) and the step (2), adding yeast beta-glucan, fructo-oligosaccharide and soybean oligosaccharide which are in corresponding weight components, and uniformly mixing to obtain the liver-protecting intestinal microecological regulator based on the liver-intestine axis.
Example 2:
the liver-protecting intestinal micro-ecological regulator based on the liver-intestinal axis of the embodiment is prepared by adopting the following raw materials by weight: 180 parts of humifuse euphorbia herb, 150 parts of ampelopsis grossedentata leaves, 220 parts of raspberry, 180 parts of purslane, 140 parts of rhizoma polygonati, 80 parts of cistanche, 13 parts of yeast beta-glucan, 20 parts of fructo-oligosaccharide and 40 parts of soybean oligosaccharide.
The preparation method of the liver-protecting intestinal microecological regulator based on the liver-intestinal axis comprises the following steps:
(1) respectively preparing herba Euphorbiae Humifusae extract, Ampelopsis grossedentata leaf extract, and Rubi fructus extract;
adding pulverized herba Euphorbiae Humifusae or Ampelopsis grossedentata leaf or Rubi fructus sieved with 24 mesh sieve into an extraction tank, adding 15 times of 40% ethanol as extraction solvent, and extracting at room temperature for 40 s; opening a communicating valve between the flash extractor and the vacuum reduced pressure extractor, allowing the feed liquid to enter the vacuum reduced pressure extractor under the action of pressure difference, extracting at 50 deg.C under 0.07MPa for 25min to obtain extractive solution; carrying out centrifugal separation on the obtained extracting solution, and then carrying out reduced pressure concentration, wherein the concentration temperature is 50 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract; spray drying the extract at a nozzle temperature of 165 ℃, an outlet temperature of 75 ℃ and a feeding speed of 14mL/min to obtain a humifuse euphorbia herb extract, a Ampelopsis grossedentata leaf extract or a raspberry extract;
(2) respectively preparing purslane extract, rhizoma polygonati extract and cistanche extract;
adding coarse powder of herba Portulacae or rhizoma Polygonati or Cistanchis herba into ball milling tank, adding 10% Na2CO3As grinding auxiliary agent, extracting for 10min, and starting condensed water in the whole process; transferring the material obtained by mechanochemical extraction into an ultrasonic extractor, adding 20 times of water as an extraction solvent, wherein the extraction temperature is 55 ℃, the extraction time is 40min, and the ultrasonic power is 250W to obtain an extracting solution; carrying out centrifugal separation on the obtained extracting solution, and then carrying out reduced pressure concentration, wherein the concentration temperature is 60 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract; spray drying the extract at nozzle temperature of 140 deg.C and outlet temperature of 65 deg.C and feeding speed of 14mL/min to obtain herba Portulacae extract or rhizoma Polygonati extract or Cistanchis herba extract;
(3) mixing the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract, the raspberry extract, the purslane extract, the polygonatum extract and the cistanche extract prepared in the step (1) and the step (2), adding yeast beta-glucan, fructo-oligosaccharide and soybean oligosaccharide which are in corresponding weight components, and uniformly mixing to obtain the liver-protecting intestinal microecological regulator based on the liver-intestine axis.
Example 3:
the liver-protecting intestinal micro-ecological regulator based on the liver-intestinal axis of the embodiment is prepared by adopting the following raw materials by weight: 210 parts of humifuse euphorbia herb, 165 parts of ampelopsis grossedentata leaves, 240 parts of raspberry, 210 parts of purslane, 140 parts of rhizoma polygonati, 100 parts of cistanche, 16 parts of yeast beta-glucan, 24 parts of fructo-oligosaccharide and 30 parts of soybean oligosaccharide.
The preparation method of the liver-protecting intestinal microecological regulator based on the liver-intestinal axis comprises the following steps:
(1) respectively preparing herba Euphorbiae Humifusae extract, Ampelopsis grossedentata leaf extract, and Rubi fructus extract;
adding pulverized herba Euphorbiae Humifusae or Ampelopsis grossedentata leaf or Rubi fructus sieved with 24 mesh sieve into an extraction tank, adding 20 times of 55% ethanol as extraction solvent, and extracting at room temperature for 50 s; opening a communicating valve between the flash extractor and the vacuum reduced pressure extractor, allowing the feed liquid to enter the vacuum reduced pressure extractor under the action of pressure difference, extracting at 50 deg.C under 0.08MPa for 25min to obtain extractive solution; carrying out centrifugal separation on the obtained extracting solution, and then carrying out reduced pressure concentration, wherein the concentration temperature is 50 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract; spray drying the extract at a nozzle temperature of 165 ℃, an outlet temperature of 75 ℃ and a feeding speed of 15mL/min to obtain a humifuse euphorbia herb extract, a ampelopsis grossedentata leaf extract or a raspberry extract;
(2) respectively preparing purslane extract, rhizoma polygonati extract and cistanche extract;
adding coarse powder of herba Portulacae or rhizoma Polygonati or Cistanchis herba into ball milling tank, adding 10% Na2CO3As grinding auxiliary agent, extracting for 4min, and starting condensed water in the whole process; transferring the material obtained by mechanochemical extraction into an ultrasonic extractor, adding 15 times of water as an extraction solvent, wherein the extraction temperature is 65 ℃, the extraction time is 30min, and the ultrasonic power is 350W to obtain an extracting solution; carrying out centrifugal separation on the obtained extracting solution, and then carrying out reduced pressure concentration, wherein the concentration temperature is 60 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract; spray drying the extract at a nozzle temperature of 160 ℃, an outlet temperature of 60 ℃ and a feeding speed of 10mL/min to obtain a purslane extract or a rhizoma polygonati extract or a cistanche extract;
(3) mixing the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract, the raspberry extract, the purslane extract, the polygonatum extract and the cistanche extract prepared in the step (1) and the step (2), adding yeast beta-glucan, fructo-oligosaccharide and soybean oligosaccharide which are in corresponding weight components, and uniformly mixing to obtain the liver-protecting intestinal microecological regulator based on the liver-intestine axis.
Example 4:
the liver-protecting intestinal micro-ecological regulator based on the liver-intestinal axis of the embodiment is prepared by adopting the following raw materials by weight: 280 parts of humifuse euphorbia herb, 210 parts of ampelopsis grossedentata leaves, 260 parts of raspberry, 280 parts of purslane, 170 parts of rhizoma polygonati, 140 parts of cistanche, 20 parts of yeast beta-glucan, 45 parts of fructo-oligosaccharide and 50 parts of soybean oligosaccharide.
The preparation method of the liver-protecting intestinal microecological regulator based on the liver-intestinal axis comprises the following steps:
(1) respectively preparing herba Euphorbiae Humifusae extract, Ampelopsis grossedentata leaf extract, and Rubi fructus extract;
adding herba Euphorbiae Humifusae or Ampelopsis grossedentata leaf or Rubi fructus which are crushed and sieved by a 50-mesh sieve into an extraction tank, adding 30 times of 60% ethanol as an extraction solvent, and extracting at room temperature for 100 s; opening a communicating valve between the flash extractor and the vacuum reduced pressure extractor, allowing the feed liquid to enter the vacuum reduced pressure extractor under the action of pressure difference, extracting at 60 deg.C under 0.1MPa for 40min to obtain extractive solution; carrying out centrifugal separation on the obtained extracting solution, and then carrying out reduced pressure concentration, wherein the concentration temperature is 50 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract; spray drying the extract at a nozzle temperature of 175 deg.C, an outlet temperature of 80 deg.C, and a feeding speed of 18mL/min to obtain herba Euphorbiae Humifusae extract, Ampelopsis grossedentata leaf extract or Rubi fructus extract;
(2) respectively preparing purslane extract, rhizoma polygonati extract and cistanche extract;
adding coarse powder of herba Portulacae or rhizoma Polygonati or Cistanchis herba into ball milling tank, adding 25% Na2CO3As grinding auxiliary agent, extracting for 10min, and starting condensed water in the whole process; transferring the material obtained by mechanochemical extraction into an ultrasonic extractor, adding 25 times of water as an extraction solvent, wherein the extraction temperature is 75 ℃, the extraction time is 60min, and the ultrasonic power is 450W to obtain an extracting solution; carrying out centrifugal separation on the obtained extracting solution, and then carrying out reduced pressure concentration, wherein the concentration temperature is 60 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract; spray drying the extract at a nozzle temperature of 160 ℃, an outlet temperature of 75 ℃ and a feeding speed of 18mL/min to obtain a purslane extract or a polygonatum extract or a cistanche extract;
(3) mixing the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract, the raspberry extract, the purslane extract, the polygonatum extract and the cistanche extract prepared in the step (1) and the step (2), adding yeast beta-glucan, fructo-oligosaccharide and soybean oligosaccharide which are in corresponding weight components, and uniformly mixing to obtain the liver-protecting intestinal microecological regulator based on the liver-intestine axis.
Comparative example 1:
the liver-protecting intestinal micro-ecological regulator based on the liver-intestinal axis of the embodiment is prepared by adopting the following raw materials by weight: 210 parts of humifuse euphorbia herb, 165 parts of ampelopsis grossedentata leaves, 240 parts of raspberry, 210 parts of purslane, 140 parts of rhizoma polygonati, 100 parts of cistanche, 16 parts of yeast beta-glucan, 24 parts of fructo-oligosaccharide and 30 parts of soybean oligosaccharide.
The preparation method of the liver-protecting intestinal microecological regulator based on the liver-intestinal axis comprises the following steps:
(1) adding humifuse euphorbia herb, Ampelopsis grossedentata leaf, raspberry, purslane, rhizoma polygonati and cistanche in corresponding weight parts into an extraction tank, adding 20 times of water, soaking for 30min, extracting by adopting a traditional extraction method, timing after boiling, and extracting for 60min to obtain an extracting solution; carrying out centrifugal separation on the obtained extracting solution, and then carrying out reduced pressure concentration, wherein the concentration temperature is 50 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract; spray drying the extract at the nozzle temperature of 165 ℃, the outlet temperature of 75 ℃ and the feeding speed of 15mL/min to obtain mixed extracts of the five raw materials;
(2) adding yeast beta-glucan, fructo-oligosaccharide and soybean oligosaccharide with corresponding weight components into the mixed extract prepared in the step (1), and uniformly mixing to obtain the liver-protecting intestinal microecological regulator based on the liver-intestinal axis.
The invention performs an administration effect test on the regulator prepared in each embodiment, and particularly relates to an efficacy evaluation test of a liver-protecting intestinal microecological regulator based on a mouse model with acute liver injury and intestinal microecological disorder.
The test method comprises the following steps: 130 Kunming mice, which weigh 20 +/-2 g and are male and female, are randomly divided into 13 groups after being adaptively fed for 1 week, and 10 mice in each group are respectively a normal control group, a model control group, a Lizhu Changle group (positive control group 1), a liver protection tablet group (positive control group 2), an example 1 group, an example 2 group, an example 3 low dose group (5.0g/kg), an example 3 medium dose group (10.0g/kg), an example 3 high dose group (20.0g/kg), an example 3 extract group (mixed with traditional Chinese medicine extracts), an example 3 prebiotic group (mixed with prebiotics), an example 4 group and an example 5 group. Except for a normal control group, each group of mice is subjected to intragastric administration with lincomycin for 0.3 mL/time, twice daily administration is carried out for three days continuously, each group of mice is subjected to intraperitoneal injection of 10% D-galactosamine with the administration dose of 0.8g/kg, and a mouse model with acute liver injury and intestinal tract micro-ecological disorder is prepared. After the model is successfully prepared, the mice of a normal control group and a model control group are filled with normal saline with equal volume, the mice of a Lizhu Changle group are filled with Lizhu Changle solution with 0.5 hundred million live bacteria/mL for 0.3 mL/time, the liver protecting tablet group, the mice of examples 1, 2, 4 and 5 and the mice of example 3 are filled with stomach after being dissolved by normal saline with the administration dosage of 10.0g/kg, the mice of example 3 are filled with stomach after being dissolved by normal saline with the administration dosage of 5.0g/kg and 20.0g/kg, the mice of example 3 are killed by cervical dislocation method after being administered once a day and continuously administered for seven days, the eyeballs of all the mice are picked to take blood, serum is separated, and the levels of endotoxin, serum alanine Aminotransferase (ALT) and glutamic Aminotransferase (AST) are measured; fixing the left liver leaves of each group of mice, preparing slices according to a frozen slicing method, performing HE staining, and observing the pathological change of liver tissues under a light microscope; fixing a small liver tissue at the position 0.5cm away from the edge of the right lobe of the liver of each group of mice by using 10% formaldehyde solution, preparing 10% liver homogenate by using cold normal saline, and measuring the content of Malondialdehyde (MDA) and superoxide dismutase (SOD) in the liver homogenate; aseptically collecting 5.0g of cecal content, adding physiological saline according to the proportion of adding 4mL of physiological saline into 1.0g of cecal content, placing the cecal content on an oscillator to oscillate for 15min to prepare suspension, centrifuging and collecting supernatant to obtain intestinal bacteria liquid, continuously diluting the intestinal bacteria liquid with the sterile physiological saline, respectively carrying out selective culture on bifidobacterium, lactobacillus, enterobacter and enterococcus, carrying out colony counting after culture, and expressing the result by a logarithmic value of the number of colonies in per gram of feces.
And (3) test results:
(1) the normal control group mice have good mental state, glossy hair color, normal ingestion, drinking and excrement and sensitive response to external stimulation in the test process; after the stomach is irrigated with lincomycin, the mice have the conditions of absentmindedness, slow movement, reduced ingestion and drinking water amount, thin and wet excrement and the like, and after D-galactosamine is injected into the abdominal cavity, the mice have listlessness, lassitude and laziness, rough and dull hair color and dull luster, are accompanied by hair removal phenomenon, and the thin and wet excrement and urine condition is further aggravated. After seven days of treatment, the positive control group and each administration group are improved to different degrees, and the mobility, the hair condition, the food intake, the water intake, the excrement dryness and the like are improved to different degrees.
(2) Compared with a normal control group, the plasma endotoxin, ALT, AST and MDA of the mice in the model control group are all obviously increased (P is less than 0.01), and the SOD is obviously reduced (P is less than 0.01), so that the successful establishment of the liver injury model is prompted. Compared with a model control group, the endotoxin, ALT, AST and MDA in the plasma of the liver protecting tablet group mice are all obviously reduced (P is less than 0.05, P is less than 0.01), and the SOD is obviously increased (P is less than 0.01); the lizhu changle group and the prebiotics group in example 3 showed no significant difference (P > 0.05) although endotoxin, ALT, AST and MDA all showed a reduction trend; SOD is in an ascending trend, but the difference is not obvious (P is less than 0.01), which indicates that the micro-ecology of the intestinal tract is independently regulated, and is helpful for improving the liver damage condition to a certain extent; the plasma endotoxin, ALT, AST and MDA of mice in the groups of example 1, example 2, example 3 and 5 are all obviously reduced (P is less than 0.05, P is less than 0.01), and the SOD is obviously increased (P is less than 0.01), which indicates that the groups can effectively improve the liver injury condition, and the comparison result among the groups shows that the extract and prebiotics are matched for use, so that the synergistic effect is good, and the efficacy of the extract prepared by the flash extraction, the vacuum decompression method and the mechanochemical synergistic ultrasonic auxiliary method is better than that of the extract prepared by the traditional extraction method. Specific test results are shown in table 1.
Table 1: comparison of the contents of endotoxin, ALT, AST, MDA and SOD in the plasma of mice in each test group: (
n＝10)
Comparison with normal control group:**p is less than 0.01; comparison with model control group:#P＜0.05，##P＜0.01
(3) the liver tissues of all groups of mice are observed to be sliced after HE staining, the liver lobules of the normal control group of mice have normal structure and no phenomena of swelling, fatty degeneration, tissue necrosis and the like, and the liver lobules and the junction area have no inflammatory cell infiltration and no fibrous connective tissue hyperplasia; compared with the normal group, the mouse in the model control group has unclear hepatic lobular boundary, has a focal necrosis area inside, has obvious hepatic cell edema and is in diffuse ballooning shape, the lobular area has hepatic cell sheet necrosis with inflammatory cell infiltration, and the convergent area also has inflammatory cell infiltration, thereby further proving that the modeling is successful; the liver protecting tablet group mouse liver lobule and manifold area structure tends to be normal, the pathological change range is reduced, and inflammatory cell infiltration is reduced; the Lizhu Changle group has slightly improved prebiotics compared with the probiotic group in the example 3, but still has swelling, large liver cell edema, balloon-like change, focal necrosis and inflammatory cell infiltration which are still obvious; in the groups of example 1, example 2, example 3 and example 4, no necrosis of liver tissues is obvious, liver cells have mild edema, and most liver cell structures are normal; the liver cells of the extract group of example 3 and the liver cells of the liver cell group of example 5 were still slightly swollen, some liver cells were slightly edematous, occasionally ballooning, focal necrosis and inflammatory cell infiltration. It is further demonstrated that the modulators of the present invention can repair damaged liver tissue and protect against liver damage.
(4) Compared with a normal control group, the number of bifidobacteria and lactobacilli in intestinal tracts of mice in the model control group is obviously reduced (P is less than 0.01), while the number of enterobacteria and enterococci is obviously increased (P is less than 0.01), which indicates that the establishment of the intestinal tract micro-ecological disorder model is successful. Compared with a model control group, the quantity of bifidobacteria and lactobacilli in intestinal tracts of mice in the lizhu Changle group is obviously increased (P is less than 0.01), and the quantity of enterobacteria and enterococci is obviously reduced (P is less than 0.05 and P is less than 0.01); example 3 the number of bifidobacteria and lactobacilli in the intestinal tract of mice in the extract group and the hepatoprotective group is increased, the number of enterobacteria and enterococci is reduced, but the difference is not significant (P is more than 0.05), which indicates that the liver-protecting medicament is used, is helpful for regulating the intestinal microecology to a certain extent, but the effect in a short time is not very obvious; the results of comparing the groups of the example 1 group, the example 2 group, the example 3 different dosage group, the example 3 prebiotic group, the example 4 group and the example 5 group show that the combination of the extract and the prebiotics has good synergistic effect and verifies that the efficacy of the extract prepared by the flash extraction and the vacuum decompression method and the mechanochemical synergistic ultrasonic wave assisted method is superior to that of the extract prepared by the traditional extraction method. Specific test results are shown in table 2.
Table 2: the results of quantitative detection of 4 bacteria in the intestinal tract of mice of each experimental group were compared (logN/g,
n＝10)
comparison with normal control group:**p is less than 0.01; comparison with model control group:#P＜0.05，##P＜0.01。
Claims (8)
1. a liver-protecting intestinal microecological regulator based on a liver-intestinal axis is characterized in that:
the preparation raw materials of the regulator are prepared from the following components in parts by weight:
100-300 parts of humifuse euphorbia herb, 60-210 parts of Ampelopsis grossedentata leaf, 100-300 parts of raspberry, 100-300 parts of purslane, 60-180 parts of rhizoma polygonati, 50-150 parts of cistanche, 5-20 parts of yeast beta-glucan, 10-50 parts of fructo-oligosaccharide and 10-60 parts of soybean oligosaccharide;
the preparation method of the regulator comprises the following steps:
(1) respectively preparing herba Euphorbiae Humifusae extract, Ampelopsis grossedentata leaf extract, and Rubi fructus extract;
(2) respectively preparing purslane extract, rhizoma polygonati extract and cistanche extract;
(3) mixing the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract, the raspberry extract, the purslane extract, the polygonatum extract and the cistanche extract prepared in the step (1) and the step (2), adding yeast beta-glucan, fructo-oligosaccharide and soybean oligosaccharide which are in corresponding weight components, and uniformly mixing to obtain the liver-protecting intestinal microecological regulator based on the liver-intestinal axis;
the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract and the raspberry extract are obtained through the following steps:
(A) flash extraction: adding the ground humifuse euphorbia herb/Ampelopsis grossedentata leaf/raspberry which are sieved by a sieve of 10-80 meshes into an extraction tank, adding 8-30 times of 20-75% ethanol serving as an extraction solvent, and extracting at room temperature for 5-120 s;
(B) vacuum decompression extraction: opening a communication valve between the flash extractor and the vacuum reduced pressure extractor, and allowing the feed liquid to enter the vacuum reduced pressure extractor under the action of pressure difference for extraction until an extracting solution is obtained;
(C) centrifugal separation: centrifuging the obtained extractive solution to obtain clarified solution;
(D) and (3) concentrating under reduced pressure: concentrating the clarified solution under reduced pressure to obtain extract;
(E) spray drying: spray drying the extract to obtain herba Euphorbiae Humifusae extract/Ampelopsis grossedentata leaf extract/Rubi fructus extract.
2. The liver-protective intestinal micro-ecology regulator based on liver-intestinal axis of claim 1, characterized in that: the regulator is prepared from the following raw materials in parts by weight:
210 parts of humifuse euphorbia herb, 165 parts of ampelopsis grossedentata leaves, 240 parts of raspberry, 210 parts of purslane, 140 parts of rhizoma polygonati, 100 parts of cistanche, 16 parts of yeast beta-glucan, 24 parts of fructo-oligosaccharide and 30 parts of soybean oligosaccharide.
3. The liver-protective intestinal micro-ecological regulator based on liver-intestinal axis according to claim 2, characterized in that: the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract and the raspberry extract are obtained by post-treatment through a flash extraction and vacuum decompression method, and the purslane extract, the sealwort extract and the cistanche extract are obtained by post-treatment through a mechanochemical and ultrasonic wave auxiliary method.
4. A method for preparing a liver-protecting intestinal microecological modulator based on the liver-intestine axis according to any one of claims 1 to 3, comprising the steps of:
(1) respectively preparing herba Euphorbiae Humifusae extract, Ampelopsis grossedentata leaf extract, and Rubi fructus extract;
(2) respectively preparing purslane extract, rhizoma polygonati extract and cistanche extract;
(3) mixing the humifuse euphorbia herb extract, the ampelopsis grossedentata leaf extract, the raspberry extract, the purslane extract, the polygonatum extract and the cistanche extract prepared in the step (1) and the step (2), adding yeast beta-glucan, fructo-oligosaccharide and soybean oligosaccharide which are in corresponding weight components, and uniformly mixing to obtain the liver-protecting intestinal microecological regulator based on the liver-intestinal axis.
5. The method for preparing a liver-protecting intestinal micro-ecology regulator based on liver-intestine axis according to claim 4, wherein: the extraction temperature of the vacuum reduced pressure extraction is 40-65 ℃, the extraction pressure is 0.05-0.1 MPa, and the extraction time is 20-40 min; the concentration temperature of the reduced pressure concentration is 50 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15.
6. The method for preparing a liver-protecting intestinal micro-ecology regulator based on liver-intestine axis according to claim 4, wherein: the spray drying process parameters are that the nozzle temperature is 160-180 ℃, the outlet temperature is 70-85 ℃, and the feeding speed is 10-20 mL/min.
7. The method for preparing a liver-protecting intestinal micro-ecology regulator based on liver-intestine axis according to claim 4, wherein: the purslane extract, the sealwort extract and the cistanche extract are obtained by the following steps
(A) Mechanical chemical extraction: adding the coarse purslane/rhizoma polygonati/herba cistanches into a ball milling tank,adding Na with the volume concentration of 5-30 percent2CO3As grinding auxiliary agent, the extraction time is 3 min-15 min, and the condensed water is started in the whole process;
(B) ultrasonic-assisted extraction: transferring the material obtained by mechanochemical extraction into an ultrasonic extractor, and adding 10-30 times of water as an extraction solvent to obtain an extracting solution;
(C) centrifugal separation: centrifuging the obtained extractive solution to obtain clarified solution;
(D) and (3) concentrating under reduced pressure: concentrating the clear liquid under reduced pressure, wherein the concentration temperature is 60 ℃, the vacuum degree is controlled to be-0.05 to-0.08 Mpa, and the relative density is controlled to be 1.05 to 1.15, so as to obtain an extract;
(E) spray drying: and (3) spray drying the extract, wherein the process parameters are that the nozzle temperature is 130-160 ℃, the outlet temperature is 60-80 ℃, and the feeding speed is 10-20 mL/min, so that the purslane extract/the rhizoma polygonati extract/the cistanche extract is obtained.
8. The method for preparing a liver-protective intestinal micro-ecology regulator based on liver-intestinal axis according to claim 7, wherein: the extraction temperature of the ultrasonic-assisted extraction is 40-80 ℃, the extraction time is 20-80 min, and the ultrasonic power is 100-500W.
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