CN108704049B - Traditional Chinese medicine composition with antioxidant function and preparation method of health food thereof - Google Patents
Traditional Chinese medicine composition with antioxidant function and preparation method of health food thereof Download PDFInfo
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- CN108704049B CN108704049B CN201810949246.1A CN201810949246A CN108704049B CN 108704049 B CN108704049 B CN 108704049B CN 201810949246 A CN201810949246 A CN 201810949246A CN 108704049 B CN108704049 B CN 108704049B
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- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/236—Ligusticum (licorice-root)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/535—Perilla (beefsteak plant)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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Abstract
The invention provides a traditional Chinese medicine composition with an antioxidant function and a preparation method of health food thereof, belonging to the technical field of functional food, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 3-45 parts of honeysuckle, 1-9 parts of purple perilla, 1-12 parts of ligusticum wallichii, 1-21 parts of cortex lycii radicis and 1-18 parts of rhizoma cyperi. The formula composition of the traditional Chinese medicine composition is based on the anti-aging theory of traditional Chinese medicine, and further adopts modern pharmacological screening analysis to take honeysuckle, perilla, ligusticum wallichii, cortex lycii radicis and rhizoma cyperi as main raw materials. The main component of the traditional Chinese medicine composition is flavonoid, and the traditional Chinese medicine composition can be used for relieving skin aging, blood vessel aging, organ deterioration and other symptoms caused by increase of free radicals in a human body. Meanwhile, toxicological tests show that the health food with the antioxidant function prepared by the invention is safe to eat and has no toxic or side effect.
Description
Technical Field
The invention relates to the technical field of functional foods, in particular to a traditional Chinese medicine composition with an antioxidant function and a preparation method of a health-care food.
Background
The organism can generate active oxygen free radicals through a chemical reaction of single electron transfer, and the types of the free radicals are various. Under normal conditions, the free radicals keep in a dynamic equilibrium state in the body, are continuously generated and are continuously eliminated by a self defense system, and a large amount of accumulation cannot be generated to influence the health of a human body. When the balance of reactive oxygen species production and clearance is disrupted, the level increases, resulting in abnormal conditions of oxidative stress in the body. Oxidative Stress (OS) was proposed by professor Soha in 1990 on the basis of free radicals. Long-term exposure to such abnormal conditions can lead to oxidative stress injury, and free radicals attack the body to produce a series of chemical reactions. The accumulation of a large amount of free radicals can also damage the structure and the function of a biological membrane, thereby causing serious chronic diseases such as cancer, cardiovascular and cerebrovascular diseases, body aging, neurodegenerative diseases, immune system diseases, diabetes, various inflammations and the like.
With the increasing pressure in modern life and work, the environmental pollution is increasingly serious, and a large amount of radiation is brought by using mobile phones, computers and the like every day, so that free radicals in human bodies are increased, the skin is aged, blood vessels are aged, organs are declined, and various diseases are caused. Therefore, the development of the health food with the antioxidant function has important significance. However, the types of health foods with antioxidant function known in the prior art are few, and the antioxidant function is not remarkable. Thus, there is a need for a product with better antioxidant capacity and more comprehensive effect.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine composition with an antioxidant function and a preparation method of a health food, and the traditional Chinese medicine composition can be used for treating, relieving and improving a series of injuries and symptoms of middle-aged and elderly people caused by organism oxidation.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a traditional Chinese medicine composition with an antioxidant function, which comprises the following raw materials in parts by weight: 3-45 parts of honeysuckle, 1-9 parts of purple perilla, 1-12 parts of ligusticum wallichii, 1-21 parts of cortex lycii radicis and 1-18 parts of rhizoma cyperi.
Preferably, the feed comprises the following raw materials in parts by weight: 4-15 parts of honeysuckle, 2-3 parts of purple perilla, 2-4 parts of ligusticum wallichii, 2-7 parts of cortex lycii radicis and 2-6 parts of rhizoma cyperi.
The invention provides an antioxidant health food of the traditional Chinese medicine composition.
Preferably, the health food is in the form of powder, granules or tablets.
The invention provides a preparation method of granules in health food, which comprises the following steps:
1) mixing honeysuckle, perilla, ligusticum wallichii, cortex lycii radicis and rhizoma cyperi with an extraction solvent, soaking and extracting, and filtering to obtain an extracting solution, wherein the extraction solvent is water or an ethanol water solution;
2) concentrating the extracting solution obtained in the step 1) to obtain an extract;
3) mixing the extract obtained in the step 2) with auxiliary materials, and performing wet granulation to obtain granules, wherein the auxiliary materials are food auxiliary materials.
Preferably, the volume ratio of the total mass of the honeysuckle flower, the perilla, the ligusticum wallichii, the cortex lycii radicis and the rhizoma cyperi in the step 1) to the extraction solvent is 1g (10-30) mL.
Preferably, the volume concentration of the ethanol water solution in the step 1) is 30-80%.
Preferably, the auxiliary materials in the step 3) comprise a filling agent and a flavoring agent.
The invention provides a preparation method of powder in health food, which comprises the following steps:
A) mixing honeysuckle, perilla, ligusticum wallichii, cortex lycii radicis and rhizoma cyperi with an extraction solvent, soaking and extracting, and filtering to obtain an extracting solution, wherein the extraction solvent is water or an ethanol water solution;
B) centrifuging the extracting solution in the step A), and taking a supernatant to obtain a refined solution;
C) and B), after spray drying the refined liquid in the step B), mixing the refined liquid with auxiliary materials to obtain powder, wherein the auxiliary materials are food auxiliary materials.
Preferably, the auxiliary material is xylitol and/or L-arabinose.
The invention has the beneficial effects that: the components of honeysuckle flower, perilla, ligusticum wallichii, cortex lycii radicis, rhizoma cyperi and the like in the traditional Chinese medicine composition with the antioxidant function are all raw materials for health-care food, wherein the honeysuckle flower and the perilla are both food and traditional Chinese medicinal materials. The formula composition of the traditional Chinese medicine composition is based on the anti-aging theory of traditional Chinese medicine, and further adopts modern pharmacological screening analysis to take honeysuckle, perilla, ligusticum wallichii, cortex lycii radicis and rhizoma cyperi as main raw materials. The honeysuckle flower in the formula is used as a monarch drug for clearing away heart-fire and lung-heat and removing skin toxicity; cortex Lycii has effects of cooling blood, removing heat, clearing lung-heat, and lowering fire; the nutgrass galingale rhizome is used as a ministerial drug for soothing the liver and relieving depression and regulating qi to widen the circulation of qi; chuan Xiong can activate blood and move qi, and Zi Su can regulate qi and strengthen qi, which are used as adjuvant drugs. The medicines are used together to play the efficacies of clearing heat, cooling blood, soothing liver and regulating qi, and are suitable for the upper symptoms of qi stagnation, blood stasis and deficient fire inflammation. The main component of the traditional Chinese medicine composition is flavonoid, and the traditional Chinese medicine composition can be used for relieving skin aging, blood vessel aging, organ deterioration and other symptoms caused by increase of free radicals in a human body. Meanwhile, toxicological tests show that the health food with the antioxidant function prepared by the invention is safe to eat and has no toxic or side effect.
Drawings
FIG. 1 Effect of samples of example 5 on mouse body weight;
FIG. 2 Effect of the sample of example 5 on the MDA content of serum;
FIG. 3 Effect of the samples of example 5 on the carbonyl content of proteins in serum;
FIG. 4 effect of sample in example 5 on SOD activity in serum;
FIG. 5 Effect of the samples of example 5 on the levels of GSH in serum.
Detailed Description
The invention provides a traditional Chinese medicine composition with an antioxidant function, which comprises the following raw materials in parts by weight: 3-45 parts of honeysuckle, 1-9 parts of purple perilla, 1-12 parts of ligusticum wallichii, 1-21 parts of cortex lycii radicis and 1-18 parts of rhizoma cyperi.
In the invention, the antioxidant composition comprises 3-45 parts of honeysuckle, preferably 4-15 parts of honeysuckle, and more preferably 5 parts of honeysuckle, wherein the honeysuckle is commercially available. The flos Lonicerae is dried bud or flower of Lonicera japonica Thunb (Lonicera japonica Thunb) of Caprifoliaceae, is sweet and cold in nature, and enters lung, heart and stomach channels, and has effects of clearing away heat and toxic materials and dispelling wind and heat. The honeysuckle flower mainly contains effective components such as volatile oil, trace elements, organic acids, triterpenoid saponins, flavonoids and the like, has the effects of bacteriostasis, antivirus, anti-inflammation, fever reduction, immune system resistance, oxidation resistance and the like, wherein the total phenols and the total flavonoids have the oxidation resistance. Extraction of honeysuckle flowerThe liquid can eliminate H in vitro of mice2O2The honeysuckle flower extract has the function of antioxidation, and can reduce the synthesis capability of neutrophils of a scalded mouse and the release capability of lysosomal enzyme. The honeysuckle is proved by research to obviously increase the T-AOC content, SOD activity, GSH content and MDA content in the serum of rats, so the honeysuckle has the function of improving the in-vivo antioxidant capacity.
In the present invention, the antioxidant composition includes 1 to 9 parts, preferably 2 to 3 parts, and more preferably 2 parts of perilla, which is commercially available. The Perillae herba is dried stem of Labiatae family Perilla frutescens (Perilla frutescens Britt), is pungent and warm, and has effects of regulating qi-flowing, relieving pain, and preventing miscarriage. Perilla frutescens contains volatile oil, terpenoids, glycosides, phenolic acids, flavonoids, etc., and has antipyretic, antimicrobial, antiemetic, antitumor, blood glucose reducing, blood lipid reducing, antioxidant, antiinflammatory, and antiallergic effects. The perilla extract can promote the reduction of ferric ions to ferrous ions and inhibit the formation of lecithin oxidation products. The perilla extract has obvious effect on scavenging free radicals and has strong oxidation resistance. The total flavonoid content is positively correlated with the clearance rate of free radicals.
In the invention, the antioxidant composition comprises 1-12 parts, preferably 2-4 parts, and more preferably 2 parts of ligusticum wallichii, and the ligusticum wallichii is sourced from the market. Rhizoma Ligustici Chuanxiong is dried rhizome of Umbelliferae plant rhizoma Ligustici Chuanxiong (Ligustrum chuanxiong Hort), is pungent and warm, and enters liver, gallbladder and pericardium channels, and has effects of promoting blood circulation and activating qi-flowing, dispelling pathogenic wind and relieving pain. Rhizoma Ligustici Chuanxiong mainly contains volatile oil, alkaloid, phenolic acid and organic acids, and has effects of scavenging free radicals, treating cardiovascular and cerebrovascular diseases, treating central nervous system, relieving pain, tranquilizing mind, treating digestive system, treating respiratory system, and treating urinary system. The rhizoma Ligustici Chuanxiong alkaloid can reduce NO and malondialdehyde content in rat body, thereby improving superoxide dismutase activity in rat body, and has antioxidant activity. It can also reduce nitric oxide synthase activity in rats, thereby reducing nerve function and brain tissue damage in rats. Ligustrazine has oxygen free radical resisting effect, and can be used for treating cerebral ischemia reperfusion injury. The total flavonoids in rhizoma Ligustici Chuanxiong have various biological activities such as antioxidant and antiaging effects. The flavonoid component in Perillae herba has effects of resisting aging, scavenging and inhibiting free radicals.
In the invention, the antioxidant composition comprises 1-21 parts, preferably 2-7 parts and more preferably 3 parts of cortex lycii radicis, wherein the cortex lycii radicis is sourced from the market. Cortex Lycii is dry root bark of fructus Lycii (Lycium chinense Mill) of Solanaceae, is sweet and cold in nature, and enters lung, liver and kidney channels, and has effects of cooling blood, removing heat, clearing lung-heat, and lowering fire. Cortex Lycii mainly contains effective components such as flavonoids and alkaloids, and has effects of regulating blood lipid, lowering blood pressure, and scavenging free radicals. The cortex Lycii extractive solution can improve free radical scavenging ability and oxidation resistance by reducing the generation of oxygen free radicals in organism, and has therapeutic effect on damage of pancreatic islet function.
In the invention, the antioxidant composition comprises 1-18 parts, preferably 2-6 parts and more preferably 2 parts of rhizoma cyperi, wherein the rhizoma cyperi is sourced from the market. The rhizoma Cyperi is dried rhizome of Cyperus rotundus L of Cyperaceae, and has effects of dispersing stagnated liver qi, relieving qi stagnation, regulating qi-flowing, relieving epigastric distention, regulating menstruation and relieving pain. The rhizoma Cyperi mainly contains volatile oil and flavonoid, and has tranquilizing, antiinflammatory, blood sugar lowering, nervous system protecting, antiallergic, and antioxidant effects. The total flavonoids in herba Aconiti lateralis Preparata has high medicinal value, and has anticancer, antitumor, cardiovascular and cerebrovascular disease resisting, antiinflammatory, analgesic, immunity regulating, blood sugar lowering, antioxidant, antiaging and radioprotective effects. In addition, the cypermethrin has better cleaning capability to free radicals and active oxygen ions. The higher the concentration of cyperus rotundus flavone, the more thoroughly the free radicals are eliminated.
The formula composition of the antioxidant composition is based on the anti-aging theory of traditional Chinese medicine, and is further determined by screening and analyzing through modern pharmacology, wherein the main raw materials are honeysuckle, perilla stem, ligusticum wallichii, cortex lycii radicis and rhizoma cyperi. The honeysuckle flower in the formula is used as a monarch drug for clearing away heart-fire and lung-heat and removing skin toxicity; cortex Lycii has effects of cooling blood, removing heat, clearing lung-heat, and lowering fire; the nutgrass galingale rhizome is used as a ministerial drug for soothing the liver and relieving depression and regulating qi to widen the circulation of qi; the rhizoma ligustici wallichii is used as an adjuvant drug for activating blood and promoting qi circulation, and the perilla stem is used as an adjuvant drug for regulating qi and regulating qi. The medicines are used together to play the efficacies of clearing heat, cooling blood, soothing liver and regulating qi, and are suitable for the upper symptoms of qi stagnation, blood stasis and deficient fire inflammation. The main component of the antioxidant composition is flavonoid, and the antioxidant composition can relieve the symptoms of skin aging, blood vessel aging, organ deterioration and the like caused by the increase of free radicals in a human body.
The invention provides an antioxidant health food comprising the traditional Chinese medicine composition in the scheme; the content of total flavonoids in the antioxidant health food is preferably more than or equal to 97.60 mg/g; the antioxidant health food preferably also comprises auxiliary materials; the auxiliary materials are food auxiliary materials.
In the present invention, the auxiliary material preferably includes a filler and a flavoring agent, and more preferably further includes a wetting agent and/or a binder; the filler is preferably one or more of starch, dextrin, pregelatinized starch, lactose, microcrystalline cellulose, sucrose, mannitol, sorbitol and inorganic salts, more preferably dextrin and/or lactose; the flavoring agent is preferably one or more of a sweetener, a spice and an essence, and is more preferably a sweetener; the sweetener is preferably sodium cyclamate and/or xylitol, more preferably sodium cyclamate; the wetting agent is preferably distilled water or ethanol water solution, and more preferably ethanol water solution with the volume fraction of 50%; the adhesive is preferably one or more of starch slurry, cellulose derivative, polyvinylpyrrolidone, sodium carboxymethyl cellulose solution, gelatin and sucrose, and more preferably starch slurry with a volume fraction of 10%.
In the invention, the dosage form of the antioxidant health food is soup, decoction, powder, granules or tablets, preferably powder, granules or tablets; the tablet is preferably a chewable tablet.
In the invention, when the dosage form of the antioxidant health food is granules, the mass ratio of the extract to the auxiliary materials is preferably (9-10) to (10-11), and is preferably 99: 101, a first electrode and a second electrode; the auxiliary materials are preferably dextrin, lactose, sodium cyclamate and starch slurry with the volume fraction of 10%; the weight parts of the extract, the dextrin, the lactose, the sodium cyclamate and the starch slurry with the volume fraction of 10 percent are preferably 49.5 parts, 16.5 parts, 33 parts, 1 part and 10 parts.
The invention provides a preparation method of a granular health food, which comprises the following steps:
1) mixing honeysuckle, perilla, ligusticum wallichii, cortex lycii radicis and rhizoma cyperi with an extraction solvent, soaking and extracting, and filtering to obtain an extracting solution, wherein the extraction solvent is water or an ethanol water solution;
2) concentrating the extracting solution obtained in the step 1) to obtain an extract;
3) mixing the extract obtained in the step 2) with auxiliary materials, and performing wet granulation to obtain granules.
In the invention, honeysuckle, perilla, ligusticum wallichii, cortex lycii radicis and rhizoma cyperi are mixed with water or ethanol water solution for soaking and extraction, and the extract is obtained by filtering; the volume ratio of the total mass of the honeysuckle, the perilla, the ligusticum wallichii, the cortex lycii radicis and the rhizoma cyperi to the extraction solvent is 1g (10-30) mL, preferably 1g (15-25) mL, and more preferably 1g (18 mL); the volume concentration of the ethanol water solution is 30-80%, preferably 40-75%, and more preferably 70%. In the invention, the extraction temperature is preferably 80-100 ℃, and more preferably 100 ℃; the extraction frequency is preferably 1-3 times, and more preferably 2 times; the extraction time is preferably 90-240 min, and more preferably 210 min; the filtration mode is preferably plate-and-frame filter pressing.
After the extracting solution is obtained, centrifuging the extracting solution, and taking supernate to obtain refined solution; the centrifuging device is preferably an ultracentrifuge; the centrifugation time is preferably 20-40 min, and more preferably 30 min; the rotating speed of the centrifugation is preferably 7000-8000 r/min, and more preferably 7500 r/min.
After the refined liquid is obtained, concentrating the refined liquid to obtain an extract; the concentration mode is preferably reduced pressure concentration; the pressure of the reduced pressure concentration is preferably-80 kPa to-40 kPa, and more preferably-60 kPa; the relative density of the extract obtained by the reduced pressure concentration is preferably 1.1-1.3, and more preferably 1.2.
After the extract is obtained, mixing the extract with auxiliary materials, and performing wet granulation to obtain granules; the wet granulation method preferably comprises the steps of mixing the extract with auxiliary materials, sequentially carrying out primary drying, crushing, primary screening, soft material preparation, granulation, secondary drying and secondary screening to obtain granules. In the present invention, the first drying method is preferably vacuum drying; the first drying temperature is preferably 60-90 ℃, and more preferably 80 ℃; the first drying time is preferably 1-3 h, and more preferably 2 h; the crushing mode is preferably grinding and crushing; the granularity of the first sieve is preferably 90-110 meshes, and more preferably 100 meshes; when the soft material is prepared, starch slurry with volume concentration of 10% is preferably adopted as an adhesive; the granulation is preferably carried out by sieving with a 14-mesh sieve by adopting an extrusion method; the second drying mode is preferably fluidized bed drying; the second drying temperature is preferably 60-90 ℃, and more preferably 80 ℃; the second drying time is preferably 10-60 min, and more preferably 30 min; the granularity of the second screening is preferably 14-18 meshes, and more preferably 16 meshes.
The granules prepared by the invention have the advantages of good taste, uniform granularity, consistent color and luster, and no phenomena of moisture absorption, softening, caking, deliquescence and the like.
In the invention, when the health food is granules, the dosage of the traditional Chinese medicine composition is preferably 3-9 g/day, and more preferably 6 g/day.
The invention provides a preparation method of a health food tablet, which comprises the following steps:
a) mixing flos Lonicerae, Perillae herba, rhizoma Ligustici Chuanxiong, cortex Lycii and rhizoma Cyperi with water or ethanol water solution, soaking and extracting, and filtering to obtain extractive solution;
b) concentrating the extracting solution obtained in the step a) to obtain an extract;
c) mixing the extract obtained in the step b) with auxiliary materials, carrying out wet granulation, and tabletting the obtained granules to obtain tablets; the auxiliary materials are the same as the auxiliary materials of the granules, and the details are not repeated here.
In the invention, honeysuckle, perilla, rhizoma ligustici wallichii, cortex lycii radicis and rhizoma cyperi are mixed with water or ethanol water solution for reflux extraction, and the extract is obtained by filtration; the volume ratio of the total mass of the honeysuckle, the perilla, the ligusticum wallichii, the cortex lycii radicis and the rhizoma cyperi to the extraction solvent is 1g (10-30) mL, preferably 1g (15-25) mL, and more preferably 1g (16 mL); the volume concentration of the ethanol water solution is 30-80%, preferably 40-75%, and more preferably 70%. In the invention, the extraction temperature is preferably 80-100 ℃, more preferably 85-95 ℃, and most preferably 90 ℃; the extraction frequency is preferably 1-3 times, and more preferably 2 times; the extraction time is preferably 90-240 min, and more preferably 150 min; the filtration mode is preferably plate-and-frame filter pressing.
After the extracting solution is obtained, concentrating the extracting solution to obtain an extract; the concentration mode is preferably reduced pressure concentration; the pressure of the reduced pressure concentration is preferably-80 kPa to-40 kPa, and more preferably-60 kPa; the relative density of the extract obtained by the reduced pressure concentration is preferably 1.1-1.3, and more preferably 1.15.
After the extract is obtained, the auxiliary materials and the extract are mixed, wet granulation is carried out, and the obtained granules are tabletted to obtain the chewable tablet. In the present invention, the wet granulation method is the same as the above-mentioned method for preparing granular health food, and is not described herein again.
In the invention, when the dosage form of the health food is a tablet, the dosage of the tablet is preferably 4-12 g/day, and more preferably 8 g/day.
The invention provides a preparation method of powder of health food in the scheme, which comprises the following steps:
A) mixing flos Lonicerae, Perillae herba, rhizoma Ligustici Chuanxiong, cortex Lycii and rhizoma Cyperi with water or ethanol water solution, soaking and extracting, and filtering to obtain extractive solution;
B) centrifuging the extracting solution in the step A), and taking a supernatant to obtain a refined solution;
C) carrying out spray drying on the refined solution in the step B), and mixing with auxiliary materials to obtain powder; the auxiliary material is a food auxiliary material, preferably xylitol and/or L-arabinose, and more preferably xylitol.
In the invention, honeysuckle, perilla, rhizoma ligustici wallichii, cortex lycii radicis and rhizoma cyperi are mixed with water or ethanol water solution for soaking and extraction, and the extract is obtained by filtering; the mass-volume ratio of the honeysuckle, the perilla, the ligusticum wallichii, the cortex lycii radicis and the rhizoma cyperi to the water or ethanol water solution is 1g (10-30) mL, preferably 1g (15-25) mL, and more preferably 1g (16 mL); the volume concentration of the ethanol water solution is 30-80%, preferably 40-75%, and more preferably 70%. In the invention, the extraction temperature is preferably 80-100 ℃, more preferably 85-95 ℃, and most preferably 90 ℃; the extraction frequency is preferably 1-3 times, and more preferably 2 times; the extraction time is preferably 50-120 min, and more preferably 60-90 min; the filtration mode is preferably plate-and-frame filter pressing.
After the extracting solution is obtained, centrifuging the extracting solution, and taking supernate to obtain refined solution; the centrifuging device is preferably an ultracentrifuge; the centrifugation time is preferably 20-40 min, and more preferably 30 min; the rotation speed of the centrifugation is preferably 8000-12000 r/min, and more preferably 10000 r/min.
After the refined liquid is obtained, spray drying is carried out on the refined liquid to obtain a dried product, and the dried product is mixed with auxiliary materials to obtain powder; the temperature of the spray drying is preferably 100-140 ℃, and more preferably 120 ℃; the mass ratio of the dried product to the auxiliary material is preferably 100: 7.
In the invention, when the dosage form of the health food is powder, the dosage of the powder is preferably 2-6 g/day, and more preferably 4 g/day.
The following examples are provided to describe the preparation method of the antioxidant Chinese medicinal composition and health food thereof in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
Weighing 3kg of honeysuckle, 3kg of perilla, 3kg of ligusticum wallichii, 7kg of cortex lycii radicis and 6kg of rhizoma cyperi, adding 330L of water, decocting and extracting for 120min, filtering, mixing filter residues with 330L of water, and decocting for 90min again. Mixing the two filtrates to obtain extractive solution. Centrifuging the extractive solution with ultracentrifuge at 7500r/min for 30 min. Concentrating the supernatant under reduced pressure of-60 kPa to obtain extract with relative density of 1.2.
Mixing the extract with 4.4kg dextrin, and performing primary drying at 90 deg.C. Pulverizing, sieving with 100 mesh sieve, making soft mass with 10 vol% starch slurry as binder, extruding, sieving with 14 mesh sieve, granulating, drying at 80 deg.C, sieving with 16 mesh sieve, and grading to obtain granule.
Example 2
Weighing 45kg of honeysuckle, 1kg of perilla, 1kg of ligusticum wallichii, 1kg of cortex lycii radicis and 1kg of rhizoma cyperi, mixing with 245L of 70 volume percent ethanol water solution, carrying out reflux extraction at 85 ℃ for 60min, filtering, mixing filter residue with 245L of 70 volume percent ethanol water solution, and carrying out reflux extraction again at 80 ℃ for 120 min. Mixing the two filtrates to obtain extractive solution. Concentrating the extractive solution under reduced pressure of-40 kPa to obtain extract with relative density of 1.15. Mixing the extract with 6kg of dextrin, 3kg of lactose and 0.2kg of sodium cyclamate, and performing primary drying at 90 ℃. Pulverizing, sieving with 100 mesh sieve, making into soft material with 50% ethanol water solution as wetting agent, squeezing, sieving with 14 mesh sieve, granulating, drying at 60 deg.C, sieving with 16 mesh sieve, grading, and tabletting.
Example 3
Weighing 50kg of honeysuckle, 20kg of perilla, 10kg of ligusticum wallichii, 30kg of cortex lycii radicis and 20kg of rhizoma cyperi, adding 1300L of water, decocting and extracting for 90min, filtering, adding 1040L of water into filter residues, and decocting and extracting for 60 min. Mixing the two filtrates to obtain extractive solution. Centrifuging the extractive solution with ultracentrifuge at 10000r/min for 30 min. Collecting supernatant as refined solution. Spray drying the refined solution at 120 deg.C, and adding xylitol 7 wt% of the dried product to obtain powder.
Example 4 Each index of the granules prepared in example 1 was measured
The fluidity, the particle size distribution and the water content of the granules are measured according to the relevant requirements of Chinese pharmacopoeia. The measurement results of various indexes of the granules are shown in table 1; the results of the quality inspection of the granules of the present invention are shown in Table 2.
TABLE 1 measurement results of various indexes of the granules
As can be seen from the results in table 2, the quality of the granules prepared in example 1 meets the requirements of "chinese pharmacopoeia".
Example 5 study of antioxidant function of the granules of example 1
80 male Kunming mice with the weight range of 18-22 g are selected and provided by the experimental animal center of Yanbian university. The test animals are adaptively fed for one week before the experiment, and the next experiment is carried out after the physical signs are well shown.
Establishing a D-galactose oxidative damage mouse model: injecting D-galactose 120 mg/kg BW into abdominal cavity of 80 mice to form an oxidative damage model, wherein the injection amount is 0.01mL/g for 1 time per day, observing hair color change and action characteristics of the mice, and continuously administering for 6 weeks. Modeling principle: the injection of D-galactose in large amount for a long time can generate excessive active oxygen and increase the content of free radicals, thereby generating a large amount of oxidation products, causing the reduction of the functions of organelles in a body, and generating various diseases.
Randomly grouping the modeled mice into 3 tested dose groups and 1 model control group based on daily dose of human, and intragastrically administering the granules prepared in example 1 with different concentrations in 3 tested dose groups of a low dose group, a medium dose group and a high dose group, wherein the dosages are 0.24g/kg BW, 0.48g/kg BW and 0.96g/kg BW respectively, and the administration times are 1 time/day and are respectively equal to 5, 10 and 20 times of the dosage taken by human; the model control group was intragastrically filled with the same volume of distilled water. D-galactose was continuously injected into the abdominal cavity of the mice while the gavage administration was performed. After the experiment, the animals were sacrificed and the eyeball bled. The mice were weighed at 0d, 10d, 20d, 30d after administration and the MDA content, SOD activity, protein carbonyl content, and GSH content were determined.
(1) The results of the effect of the samples on the body weight of the mice are shown in table 3 and fig. 1. The results show that: the weight of the mice is continuously increased after the administration for 0-30 d, and the weight of the mice after the administration for 0, 10, 20 and 30d has no significant difference compared with the model control group in each administration group, which indicates that the weight of the mice is not affected by the test sample.
TABLE 3 Effect of granules on mouse body weight
(LG: low dose group; MG: medium dose group; HG: high dose group; MC: model control group)
(2) The results of the effect of the samples on the MDA content in the serum are shown in table 4, fig. 2, showing: compared with the MC group, the MDA content of the LG, the MG and the HG group is reduced, and the obvious difference (p is less than 0.05) is provided, which indicates that the tested sample can obviously reduce the MDA content in vivo and reduce the peroxidation damage after the mice are subjected to the oxidative damage. The result shows that the medicine has the function of reducing lipid peroxidation, and the result of the index of the experiment item is positive.
TABLE 4 MDA content determination results
Note: significant differences between each administered group and the model control group, p <0.05, are shown in the table below
(3) The results of the effect of the samples on the content of protein carbonyl in serum are shown in table 5 and fig. 3, and the results show that: compared with the MC group, the protein carbonyl content of the LG, the MG and the HG group is obviously reduced and has significant difference (p is less than 0.05), which indicates that the granules prepared in example 1 have the capability of repairing the oxidative damage of the protein. The result of the index experiment is positive.
TABLE 5 measurement results of protein carbonyl content
(4) The results of the effect of the samples on the activity of SOD in serum are shown in Table 6 and FIG. 4, and the results show that: compared with the MC group, the SOD activities of the LG, the MG and the HG group are increased, and the determination result has significance (p is less than 0.05). Along with the increase of the dosage, the SOD activity is increased, which shows that the tested sample can improve the activity of the antioxidant enzyme, and the result of the experimental index is positive.
Table 6 SOD activity assay results (x ± s, n ═ 10)
(5) The results of the effect of the samples on the serum GSH content are shown in table 7, fig. 5, and show: compared with the group of LG, MG and HG, the content of GSH is reduced, and the determination result has significance (p is less than 0.05). With increasing dose, GSH content of three groups, LM, MG, HG, varied significantly. It was shown that the GSH content in vivo was significantly increased in mice given the granules by gavage compared to the model control group, with the highest increase in the high dose group. The test sample is shown to have the function of increasing antioxidant substances, and the result of the experimental index is negative.
TABLE 7 GSH content determination results
From the above, the results of the four indexes of MDA content, SOD activity, protein carbonyl content, and GSH content all show positive, and it can be determined that the antioxidant animal experiment result of the granule prepared in example 1 is positive.
From the above embodiments, it can be seen that the present invention provides a Chinese medicinal composition with an antioxidant function and a method for preparing a health food thereof, wherein granules of the health food have the effects of reducing the content of protein carbonyl and the content of MDA, and increasing the anti-SOD activity and the content of GSH, and the results show that the health food is helpful for mice to repair peroxidation damage caused by D-galactose. According to the evaluation and judgment standard of the health food function, the traditional Chinese medicine composition and the health food thereof can be determined to have the antioxidant function.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A traditional Chinese medicine composition with an antioxidant function is composed of the following raw materials in parts by weight: 3 parts of honeysuckle, 3 parts of purple perilla, 3 parts of ligusticum wallichii, 7 parts of cortex lycii radicis and 6 parts of rhizoma cyperi.
2. An antioxidant health food comprising the Chinese medicinal composition of claim 1.
3. The antioxidant health food as claimed in claim 2, wherein the health food is in the form of powder, granule or tablet.
4. The method for preparing granules in health food according to claim 3, comprising the steps of:
1) Mixing honeysuckle, perilla, ligusticum wallichii, cortex lycii radicis and rhizoma cyperi with an extraction solvent, soaking and extracting, and filtering to obtain an extracting solution, wherein the extraction solvent is water or an ethanol water solution;
2) Concentrating the extracting solution obtained in the step 1) to obtain an extract;
3) Mixing the extract obtained in the step 2) with auxiliary materials, and performing wet granulation to obtain granules, wherein the auxiliary materials are food auxiliary materials.
5. The preparation method of the granules according to claim 4, wherein the volume ratio of the total mass of the honeysuckle flower, the perilla, the ligusticum wallichii, the cortex lycii radicis and the rhizoma cyperi in the step 1) to the extraction solvent is 1g (10-30) mL.
6. The preparation method of the granules according to claim 4 or 5, wherein the volume concentration of the ethanol aqueous solution in the step 1) is 30-80%.
7. The preparation method of the granules according to claim 6, wherein the auxiliary materials in the step 3) comprise a filler and a flavoring agent.
8. A method for preparing a powder for health food according to claim 3, comprising the steps of:
A) mixing honeysuckle, perilla, ligusticum wallichii, cortex lycii radicis and rhizoma cyperi with an extraction solvent, soaking and extracting, and filtering to obtain an extracting solution, wherein the extraction solvent is water or an ethanol water solution;
B) centrifuging the extracting solution in the step A), and taking a supernatant to obtain a refined solution;
C) and B), after spray drying the refined liquid in the step B), mixing the refined liquid with auxiliary materials to obtain powder, wherein the auxiliary materials are food auxiliary materials.
9. The method for preparing a powder according to claim 8, wherein: the auxiliary material is xylitol and/or L-arabinose.
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