CN110960632B - Formula of sleep improving product and preparation method thereof - Google Patents
Formula of sleep improving product and preparation method thereof Download PDFInfo
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- CN110960632B CN110960632B CN201911086137.2A CN201911086137A CN110960632B CN 110960632 B CN110960632 B CN 110960632B CN 201911086137 A CN201911086137 A CN 201911086137A CN 110960632 B CN110960632 B CN 110960632B
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a formula of a product for improving sleep and a preparation method thereof, wherein the formula comprises the following raw materials in parts by weight: 10-15 parts of spina date seed, 6-12 parts of angelica sinensis, 6-12 parts of lily and 6-12 parts of dendrobium officinale; according to the invention, the effective components of the Chinese herbal medicines are extracted, and the different effects of the four components are utilized to generate a synergistic effect, so that a compound product with the effects of nourishing yin, promoting the secretion of saliva or body fluid, calming the heart and soothing the nerves is formed.
Description
Technical Field
The invention relates to a health-care product, in particular to a formula of a sleep-improving product and a preparation method thereof.
Background
Chinese sleep research will issue that the incidence of insomnia of adults in China is as high as 38.2%. Insomnia can cause anxiety, depression, or fear in the patient and lead to a decrease in mental efficiency and hamper social functioning. The insomnia causes abnormal expression of genes of biological clocks, further causes dysfunction of adjustment of body nerve and body fluid, can cause various diseases such as hypertension, heart disease, cerebral apoplexy, diabetes, Alzheimer disease, heart failure and the like, and seriously threatens life. The health-care food is one of the main rehabilitation modes of insomnia sub-health people, and the health-care food for improving the sleep function becomes a choice for more people due to the rise of the prevention health-care consciousness of people.
In the internal medicine of traditional Chinese medicine, insomnia is divided into 6 types: the symptoms of liver depression transforming into fire, heart fire hyperactivity, yin deficiency fire hyperactivity, phlegm heat disturbance, deficiency of heart-gallbladder qi, and deficiency of both heart and spleen. The Chinese medicinal composition has the effects of tonifying yin, removing fire, tonifying qi, nourishing blood, tonifying spleen, nourishing heart, soothing liver, regulating qi, tonifying kidney, replenishing vital essence, clearing heart fire, soothing nerves, removing food retention, harmonizing stomach, balancing viscera and qi and blood, coordinating yin and yang, and stabilizing mind and heart.
The health food for insomnia comprises two types of compounds and traditional Chinese medicines. The compound health-care product for treating insomnia mainly comprises brain platinum, the main component of the compound health-care product is melatonin, and the compound health-care product has the advantages of quick response, definite effect and the like, but can easily cause dependence reaction after being taken for a long time, and influences the secretion of the melatonin, particularly tolerance, dependence, endocrine disturbance and the like. The traditional Chinese medicine health care explains 'regulating the adverse effect of the traditional Chinese medicine and removing the harm of the traditional Chinese medicine', finds out the reason of insomnia, and helps insomniacs to recover sleep by regulating organs of the whole body; the market needs a traditional Chinese medicine product for conditioning viscera and improving sleep, and the invention solves the problem.
Disclosure of Invention
In order to solve the defects of the prior art, the invention aims to provide a formula of a sleep improving product and a preparation method thereof.
In order to achieve the above object, the present invention adopts the following technical solutions:
the formula of a product for improving sleep comprises the following raw materials in parts by weight: 10-15 parts of spina date seed, 6-12 parts of angelica sinensis, 6-12 parts of lily and 6-12 parts of dendrobium officinale.
The formula of the sleep-improving product comprises, by mass, 10 parts of spina date seeds, 6 parts of angelica sinensis, 6 parts of lily and 6 parts of dendrobium officinale.
A preparation method of a sleep-improving product comprises the following steps:
preparing materials according to a raw material formula; the formula comprises the following raw materials in parts by weight: 10-15 parts of spina date seed, 6-12 parts of angelica sinensis, 6-12 parts of lily and 6-12 parts of dendrobium officinale;
preparing the wild jujube seed extract, extracting,
heating and refluxing semen Ziziphi Spinosae raw material with solvent, filtering, and concentrating to obtain semen Ziziphi Spinosae extract;
preparing an extract of Chinese angelica root,
extracting radix Angelicae sinensis with solvent, filtering, and concentrating to obtain radix Angelicae sinensis extract;
preparing a lily extract, extracting the lily with water,
extracting Bulbus Lilii raw material with solvent, filtering, and concentrating to obtain Bulbus Lilii extract;
preparing the dendrobium officinale extract, namely preparing the dendrobium officinale extract,
extracting herba Dendrobii with solvent, filtering, and concentrating to obtain herba Dendrobii extract;
mixing the extracts to obtain the final product.
In the preparation method of the sleep-improving product,
preparing materials according to a raw material formula; the formula comprises the following raw materials in parts by weight: 10-15 parts of spina date seed, 6-12 parts of angelica sinensis, 6-12 parts of lily and 6-12 parts of dendrobium officinale;
preparing the wild jujube seed extract, extracting,
heating and refluxing semen Ziziphi Spinosae raw material with 8 times of 70% ethanol, filtering, and concentrating to obtain semen Ziziphi Spinosae extract;
preparing an extract of Chinese angelica root,
soaking radix Angelicae sinensis in 6 times of water, extracting, filtering, and concentrating to obtain radix Angelicae sinensis extract;
preparing a lily extract, extracting the lily with water,
extracting Bulbus Lilii raw material with 7.5 times of 80% ethanol, filtering, and concentrating to obtain Bulbus Lilii extract;
preparing the dendrobium officinale extract, namely preparing the dendrobium officinale extract,
taking a dendrobium officinale raw material, soaking and extracting the dendrobium officinale raw material with 80 times of water, filtering and concentrating for later use to obtain a dendrobium officinale extract;
mixing the extracts to obtain the final product.
In the preparation method of the sleep-improving product,
the specific process for preparing the spina date seed extract comprises the following steps:
taking semen Ziziphi Spinosae raw material, extracting with 8 times of 70% ethanol under reflux at 70 deg.C twice, each for 1.5 hr, and filtering; mixing filtrates, concentrating under reduced pressure at 60 deg.C and 0.09MPa for 1 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; and crushing the dry extract, and sieving the crushed dry extract with a 60-mesh sieve to obtain extract powder for later use, thereby obtaining the spina date seed extract.
In the preparation method of the sleep-improving product,
the specific process for preparing the angelica extract comprises the following steps:
extracting radix Angelicae sinensis with 6 times of water at 90 deg.C for two times (2 hr each time), and filtering; mixing filtrates, concentrating under reduced pressure at 80 deg.C and 0.09MPa for 3 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; pulverizing the dry extract, and sieving with 60 mesh sieve to obtain extract powder.
In the preparation method of the sleep-improving product,
the specific process for preparing the lily extract comprises the following steps:
taking lily raw material, condensing and refluxing for two times at 60 ℃ by using 7.5 times of 80% ethanol, extracting for 3 hours each time, and filtering; mixing filtrates, concentrating under reduced pressure at 60 deg.C and 0.09MPa for 1 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; and crushing the dry extract, and sieving the crushed dry extract with a 60-mesh sieve to obtain extract powder for later use, thereby obtaining the lily extract.
In the preparation method of the sleep-improving product,
the specific process for preparing the dendrobium officinale extract comprises the following steps:
leaching the dendrobium officinale raw material twice with 80 times of water at 90 ℃ for 1h each time, and filtering; mixing filtrates, concentrating under reduced pressure at 80 deg.C and 0.09MPa for 2 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; and crushing the dry extract, and sieving the crushed dry extract with a 60-mesh sieve to obtain extract powder for later use, thereby obtaining the dendrobium officinale extract.
The invention has the advantages that:
according to the invention, different Chinese herbal medicines are extracted by different extraction methods, so that the obtained extract has high activity and high yield;
the components of the extract are matched with each other, so that the extract has a synergistic effect, and can improve sleep, prolong the sleep time of animals and shorten the sleep latency time of the animals;
the product prepared by the formula can be obtained by extracting through a conventional process, is low in cost, and is rich in applicable dosage forms, such as tablets, capsules, granules, powder, oral liquid, honey paste and the like.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
A preparation method of a sleep-improving product comprises the following steps:
preparing the wild jujube seed extract, extracting,
heating and refluxing semen Ziziphi Spinosae raw material with solvent, filtering, and concentrating to obtain semen Ziziphi Spinosae extract; preferably, the solvent is 8 times of 70% ethanol;
preferably, the specific process for preparing the spina date seed extract comprises the following steps: taking semen Ziziphi Spinosae raw material, extracting with 8 times of 70% ethanol under reflux at 70 deg.C twice, each for 1.5 hr, and filtering; mixing filtrates, concentrating under reduced pressure at 60 deg.C and 0.09MPa for 1 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; crushing the dry extract, and sieving the crushed dry extract with a 60-mesh sieve to obtain extract powder for later use to obtain the spina date seed extract; the semen Ziziphi Spinosae extract contains spina date seed saponin (200mg/g-250 mg/g).
Preparing an extract of Chinese angelica root,
extracting radix Angelicae sinensis with solvent, filtering, and concentrating to obtain radix Angelicae sinensis extract; as a preference, the solvent is selected from 6 times of water;
preferably, the specific process for preparing the angelica extract comprises the following steps: extracting radix Angelicae sinensis with 6 times of water at 90 deg.C for two times (2 hr each time), and filtering; mixing filtrates, concentrating under reduced pressure at 80 deg.C and 0.09MPa for 3 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; pulverizing the dry extract, and sieving with 60 mesh sieve to obtain extract powder; the radix Angelicae sinensis extract contains polysaccharide (85mg/g-95 mg/g).
Preparing a lily extract, extracting the lily with water,
extracting Bulbus Lilii raw material with solvent, filtering, and concentrating to obtain Bulbus Lilii extract; preferably, the solvent is 7.5 times of 80% ethanol;
as a preferred method, the specific process for preparing the lily extract comprises: taking lily raw material, condensing and refluxing for two times at 60 ℃ by using 7.5 times of 80% ethanol, extracting for 3 hours each time, and filtering; mixing filtrates, concentrating under reduced pressure at 60 deg.C and 0.09MPa for 1 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; crushing the dry extract, and sieving the crushed dry extract with a 60-mesh sieve to obtain extract powder for later use to obtain a lily extract; the Bulbus Lilii extract contains Bulbus Lilii saponin (100mg/g-130 mg/g).
Preparing the dendrobium officinale extract, namely preparing the dendrobium officinale extract,
extracting herba Dendrobii with solvent, filtering, and concentrating to obtain herba Dendrobii extract; as a preference, the solvent is 80 times of water; as an optimization, the specific process for preparing the dendrobium officinale extract comprises the following steps: leaching the dendrobium officinale raw material twice with 80 times of water at 90 ℃ for 1h each time, and filtering; mixing filtrates, concentrating under reduced pressure at 80 deg.C and 0.09MPa for 2 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; crushing the dry extract, and sieving the crushed dry extract with a 60-mesh sieve to obtain extract powder for later use to obtain the dendrobium officinale extract; the herba Dendrobii extract contains Dendrobium officinale polysaccharide (800mg/g-900 mg/g).
Mixing according to the formula; the formula comprises the following raw materials in parts by weight: 10-15 parts of spina date seed extract, 6-12 parts of angelica sinensis extract, 6-12 parts of lily extract and 6-12 parts of dendrobium officinale extract.
Pharmacology:
zizyphi Spinosae semen is sweet, sour and neutral in nature, and enters liver, gallbladder and heart meridians. Has the functions of nourishing heart, tonifying liver, calming heart, tranquilizing, arresting sweating and promoting the production of body fluid. The semen Ziziphi Spinosae extract comprises fatty oil, flavonoids, triterpenes, alkaloids and organic acids. The record of compendium of materia medica states that the jujube kernel is sour and sour, so it is mainly indicated for liver disease, cold and heat accumulation of qi, chronic diarrhea, and pain under the umbilicus. It is sweet and moist in property, so it is used to treat insomnia due to gallbladder deficiency, polydipsia and sweating; unprocessed herbs for gallbladder heat and good sleep are also indicated for jueyin and shaoyang. Modern pharmacological research shows that the wild jujube seed extract has the functions of tranquilizing and hypnotizing, calming, resisting convulsion, easing pain, reducing temperature, lowering blood pressure, strengthening heart and resisting anoxia. Saponins, flavonoids and alkaloids of semen Ziziphi Spinosae extract are effective components of semen Ziziphi Spinosae extract, and have sedative and hypnotic effects.
Dang Gui is sweet, pungent and warm in nature. It enters liver, heart and spleen meridians. Has the functions of enriching blood, promoting blood circulation, regulating menstruation, relieving pain, moistening intestine and relaxing bowels. The Chinese angelica extract is recorded in Ben Cao gang mu for treating headache, pain of heart and abdomen, moistening intestines, stomach, muscles, bones and skin, treating carbuncle-abscess, expelling pus, relieving pain, and tonifying blood. Modern pharmacological studies show that the angelica extract can reduce myocardial excitability; dilate coronary artery and increase coronary flow; reducing oxygen consumption of cardiac muscle and lowering blood pressure; has antiarrhythmic, antiatherosclerotic and microcirculation improving effects. The chemical components of the angelica extract are mainly divided into volatile oil part and water-soluble part, the volatile oil part comprises neutral, acidic and phenolic oil 3 parts, wherein ligustilide is the main component of the volatile oil part of the angelica extract, and the water-soluble part mainly comprises ferulic acid, polysaccharide and the like. The organic acid is one of the main active ingredients of the angelica sinensis extract, and the compound is mainly ferulic acid, so that the angelica sinensis extract has the effects of effectively protecting cardiac muscle cells, tranquilizing, protecting liver and benefiting gallbladder. Ferulic acid can inhibit hypoxia-induced endothelin release, increase nuclear transcription inhibitory protein expression and cerebral cortex microvascular endothelial cell (BMEC) activity, and protect rat cerebral cortex BMEC from hypoxia injury. The polysaccharides extracted from radix Angelicae sinensis can affect hematopoietic system of animal body, and stimulate proliferation of hematopoietic progenitor cells.
Bulbus Lilii has effects of moistening lung, tranquilizing mind, caring skin, and can be used for treating chronic cough due to yin deficiency, vexation, pavor, absentmindedness, insomnia and dreaminess. The record of Shen nong Ben Cao Jing: the lily extract has sweet taste and mild nature, and is mainly used for treating pathogenic factors abdominal distension, cardiodynia, facilitating urination and defecation, and tonifying middle-jiao and Qi. At present, scholars at home and abroad totally separate or detect more than 100 compounds from plant bulbs of 3 medicinal lily extracts, wherein the compounds mainly comprise steroid saponins and phenolic acid glyceride compounds, and in addition, the scholars also contain components such as sterol, flavone, phenylpropanoid, alkaloid, polysaccharide and the like. Modern pharmacological experimental research shows that the lily extract and the effective components thereof have various pharmacological activities, including cough relieving, phlegm eliminating, tranquilizing, hypnosis, immunoregulation, anti-tumor, anti-oxidation, anti-inflammation, anti-hypoxic stress injury, anti-depression, blood sugar reducing, bacteriostasis and the like.
Dendrobium officinale is a dried stem of an orchid plant, is a rare traditional Chinese medicine, is listed as the head of the nine-large Chinese mesona in the Collection, is well known as the gold in the medicine, and has the effects of promoting the production of body fluid, tonifying stomach, nourishing yin, clearing heat, prolonging life and enhancing immunity. The book records dendrobium nobile in Shen nong Ben Cao Jing: "sweet and mild". Mainly injure the middle-jiao, remove arthralgia, descend qi, tonify five internal organs, strengthen yin and make the stomach and intestine weak, and can be taken for a long time. Modern pharmacological research shows that the dendrobium officinale extract has various chemical components, the main active components are dendrobium polysaccharide, dendrobine, amino acid, stilbenes and derivatives thereof and various volatile components, and recently 18 new compounds are found from the dendrobium officinale extract. The dendrobium nobile lindl has the functions of nourishing skin, reducing blood sugar, resisting tumor, protecting liver, benefiting gallbladder, tonifying five internal organs, strengthening body, nourishing yin, and enhancing the functions of spleen and stomach after being taken for a long time, thereby enabling the human body to be flexible, enhancing the immunity and prolonging the life.
The effect of improving sleep of the product is verified through experiments;
preparing a product for experiment;
the needed raw materials of the wild jujube seed extract, the angelica extract, the lily extract and the dendrobium officinale extract can be purchased from commercial sources.
The above raw material extract can be prepared by the following method.
1. Preparation of wild jujube seed extract
Extracting semen Ziziphi Spinosae material selected by quality standard test with 8 times of 70% ethanol under reflux at 70 deg.C for two times, each for 1.5 hr, and filtering; mixing filtrates, concentrating under reduced pressure at 60 deg.C and 0.09MPa for 1 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; the dry extract is crushed and sieved by a 60-mesh sieve to obtain extract powder for later use.
2. Preparation of Angelica sinensis extract
Extracting radix Angelicae sinensis with 6 times of water at 90 deg.C twice for 2 hr, and filtering; mixing filtrates, concentrating under reduced pressure at 80 deg.C and 0.09MPa for 3 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; the dry extract is crushed and sieved by a 60-mesh sieve to obtain extract powder for later use.
3. Preparation of Lily extract
Extracting radix Angelicae sinensis with 7.5 times of 80% ethanol at 60 deg.C for 3 hr twice, and filtering; mixing filtrates, concentrating under reduced pressure at 60 deg.C and 0.09MPa for 1 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; the dry extract is crushed and sieved by a 60-mesh sieve to obtain extract powder for later use.
4. Preparing dendrobium officinale extract
Extracting radix Angelicae sinensis with 80 times of water at 90 deg.C for two times (each for 1 hr), and filtering; mixing filtrates, concentrating under reduced pressure at 80 deg.C and 0.09MPa for 2 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; the dry extract is crushed and sieved by a 60-mesh sieve to obtain extract powder for later use.
5. Mixing the obtained mixed spina date seed extract, the angelica sinensis extract, the lily extract and the dendrobium officinale extract according to nine different formulas obtained by orthogonal experiments;
the nine formulations are as follows:
example 1: 10g of spina date seed, 6g of angelica, 6g of lily and 6g of dendrobium officinale
Example 2: 12.5g of spina date seed, 9g of angelica, 9g of lily and 6g of dendrobium officinale
Example 3: 15g of spina date seed, 12g of angelica, 12g of lily and 6g of dendrobium officinale
Example 4: 15g of spina date seed, 6g of angelica, 9g of lily and 9g of dendrobium officinale
Example 5: 10g of spina date seed, 9g of angelica, 12g of lily and 9g of dendrobium officinale
Example 6: 12.5g of spina date seed, 12g of angelica, 6g of lily and 9g of dendrobium officinale
Example 7: 12.5g of spina date seed, 6g of angelica, 12g of lily and 12g of dendrobium officinale
Example 8: 15g of spina date seed, 9g of angelica, 6g of lily and 12g of dendrobium officinale
Example 9: 10g of spina date seed, 12g of angelica, 9g of lily and 12g of dendrobium officinale
And (3) verification experiment: functional evaluation for sleep improvement of the present invention
1 materials and methods
1.1 sample: the product obtained by mixing the formulas of the above 9 examples
1.2 Experimental animals: the clean-grade ICR mice are female, have the weight of 18-22 g, and are provided by Shanghai Jihui laboratory animal feeding company Limited, and the total number of the mice is 100.
1.3 dose selection: in this experiment, 100 animals were randomly divided by body weight into 10 experimental groups, one negative control group (distilled water) and 9 example groups, each of which was 10 animals. The samples were weighed daily for one week after acclimatization, and the samples were gavaged for 30 days at 10-fold dosage in human body according to "health food testing and evaluation specifications" (2003 edition) and orally at 2.0mL/100 gBW.
1.4 Main reagents: pentobarbital sodium (analytically pure, Shanghai Banyan biotechnology Limited)
1.5 test methods:
1.5.1 direct sleep test: after the stomach is not irrigated again, whether the sleep phenomenon appears is observed, and the sleep takes the disappearance of the righting reflex as an index. When the mouse is placed in the back lying position, the mouse can turn over to the right position immediately, if the mouse cannot turn over for more than 60s, the turning over reflection is considered to disappear, and the mouse enters sleep. The recovery of the righting reflex is the awakening of the animal, the period from disappearance of the righting reflex to recovery is the sleeping time of the animal, and the number of sleeping animals and the sleeping time of the control group and each dosage group are recorded.
1.5.2 test for prolonging the sleep time of sodium pentobarbital: after the last gastric lavage for 15min, 50 mg/kg is injected into the abdominal cavity of each group of animals-1bw the injection amount of the sodium pentobarbital is 0.2mL/20gbw, and whether the test sample prolongs the sleep time of the sodium pentobarbital is observed by taking the disappearance of the righting reflex for more than 1 minute as a sleep-falling judgment standard.
1.5.3 sub-threshold dose hypnotic test of sodium pentobarbital: injecting 40 mg/kg into abdominal cavity of each group of animals after last intragastric administration for 15min-1The injection amount of bw sodium pentobarbital is 0.2mL/20gbw, the number of animals falling asleep within 30 minutes is recorded by taking the disappearance of righting reflex for more than 1 minute as the standard for judging falling asleep.
1.5.4 pentobarbital sodium sleep latency test: injecting 40 mg/kg into abdominal cavity of each group of animals after last intragastric administration for 15min-1The injection amount of bw sodium pentobarbital is 0.2mL/20gbw, the influence of the sleep latency of the sample sodium pentobarbital is observed by taking the disappearance of righting reflex for more than 1 minute as the sleep onset judgment standard.
2 results of the test
2.1 the effect of the test substances on sleep time results are shown in Table 1;
TABLE 1 Effect of test substances on sleep in mice
As can be seen from Table 1, no direct sleep was observed in any of the groups. In the test for the effect of prolonging the sleep time of sodium pentobarbital, the animals in groups 1, 2, 3, 4 and 6 had an increased sleep time compared to the control group, and the difference was statistically significant (P < 0.05). In the test of the effect on the sleep incidence of the subthreshold dose of sodium pentobarbital, the difference in the sleep incidence of the animals in groups 1, 2, 3, 4, 5 and 6 was statistically significant (P <0.05) compared to the control group. In the test of the effect on sleep latency of sodium pentobarbital, the animals in groups 1, 2, 3, 4 and 6 had a shorter time to sleep latency compared to the control group, and the difference was statistically significant (P < 0.05). The health care product prepared in example 1 of the present application is suggested to have the effect of improving the sleep function of animals according to the judgment standard of "health food inspection and evaluation technical specification" (2003 edition).
And (4) analyzing results: from the above results, the optimal ratio of the active components of the invention is: 10g of spina date seed, 6g of angelica sinensis, 6g of lily and 6g of dendrobium officinale.
Experiment II, a formula component experiment proves that the four components have a synergistic effect;
TABLE 2 Experimental groups
TABLE 3 results of the experiment
And (4) analyzing results: according to the results, the optimal formula of the product for improving sleep is the formula of the wild jujube seed, the Chinese angelica, the lily and the dendrobium officinale, and the four medicinal materials have synergistic effects and cannot be used.
The invention adopts a plurality of medicinal and edible Chinese herbal medicines to extract effective components, combines the characteristics of the traditional Chinese medicine with the research of modern medicine, utilizes the different efficacies of the four components to generate synergistic action, and forms a compound product with the functions of nourishing yin, promoting the secretion of saliva or body fluid, and calming the heart and tranquilizing the mind.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.
Claims (6)
1. The product for improving sleep is characterized by comprising the following raw materials in parts by mass: 10 parts of spina date seeds, 6 parts of Chinese angelica, 6 parts of lily and 6 parts of dendrobium officinale; the wild jujube seed is a wild jujube seed extract, wherein the wild jujube seed extract is prepared by heating, refluxing and extracting wild jujube seed raw materials by using 70% ethanol in an amount which is 8 times that of the wild jujube seed raw materials, filtering and concentrating the extract for later use; the angelica is an angelica extract which is prepared by taking 6 times of angelica raw material and carrying out water extraction, filtration and concentration for later use; the lily is a lily extract, and the lily extract is obtained by extracting a lily raw material with 7.5 times of 80% ethanol, filtering and concentrating for later use; the dendrobium officinale is a dendrobium officinale extract, and the dendrobium officinale extract is prepared by taking a dendrobium officinale raw material, soaking and extracting the dendrobium officinale raw material with 80 times of water, filtering and concentrating for later use.
2. The method for preparing a product for improving sleep as claimed in claim 1, comprising the following steps:
preparing materials according to a raw material formula; the formula comprises the following raw materials in parts by mass: 10 parts of spina date seeds, 6 parts of Chinese angelica, 6 parts of lily and 6 parts of dendrobium officinale;
preparing the wild jujube seed extract, extracting,
heating and refluxing semen Ziziphi Spinosae raw material with 8 times of 70% ethanol, filtering, and concentrating to obtain semen Ziziphi Spinosae extract;
preparing an extract of Chinese angelica root,
soaking radix Angelicae sinensis in 6 times of water, extracting, filtering, and concentrating to obtain radix Angelicae sinensis extract;
preparing a lily extract, extracting the lily with water,
extracting Bulbus Lilii raw material with 7.5 times of 80% ethanol, filtering, and concentrating to obtain Bulbus Lilii extract;
preparing the dendrobium officinale extract, namely preparing the dendrobium officinale extract,
taking a dendrobium officinale raw material, soaking and extracting the dendrobium officinale raw material with 80 times of water, filtering and concentrating for later use to obtain a dendrobium officinale extract;
mixing the extracts to obtain the final product.
3. The method for preparing a product for improving sleep according to claim 2,
the specific process for preparing the spina date seed extract comprises the following steps:
taking semen Ziziphi Spinosae raw material, extracting with 8 times of 70% ethanol under reflux at 70 deg.C twice, each for 1.5 hr, and filtering; mixing filtrates, concentrating under reduced pressure at 60 deg.C and 0.09MPa for 1 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; and crushing the dry extract, and sieving the crushed dry extract with a 60-mesh sieve to obtain extract powder for later use, thereby obtaining the spina date seed extract.
4. The method for preparing a product for improving sleep according to claim 2,
the specific process for preparing the angelica extract comprises the following steps:
extracting radix Angelicae sinensis with 6 times of water at 90 deg.C for two times (2 hr each time), and filtering; mixing filtrates, concentrating under reduced pressure at 80 deg.C and 0.09MPa for 3 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; pulverizing the dry extract, and sieving with 60 mesh sieve to obtain extract powder.
5. The method for preparing a product for improving sleep according to claim 2,
the specific process for preparing the lily extract comprises the following steps:
taking lily raw material, condensing and refluxing for two times at 60 ℃ by using 7.5 times of 80% ethanol, extracting for 3 hours each time, and filtering; mixing filtrates, concentrating under reduced pressure at 60 deg.C and 0.09MPa for 1 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; and crushing the dry extract, and sieving the crushed dry extract with a 60-mesh sieve to obtain extract powder for later use, thereby obtaining the lily extract.
6. The method for preparing a product for improving sleep according to claim 2,
the specific process for preparing the dendrobium officinale extract comprises the following steps:
leaching the dendrobium officinale raw material twice with 80 times of water at 90 ℃ for 1h each time, and filtering; mixing filtrates, concentrating under reduced pressure at 80 deg.C and 0.09MPa for 2 hr, and concentrating to obtain soft extract; then freeze-drying in vacuum to obtain dry extract, wherein the sample temperature is-24 ℃, the cold hydrazine temperature is-96 ℃, the pressure is 1Pa, and the time is 24 h; and crushing the dry extract, and sieving the crushed dry extract with a 60-mesh sieve to obtain extract powder for later use, thereby obtaining the dendrobium officinale extract.
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