CN108699515A - 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞 - Google Patents

由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞 Download PDF

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CN108699515A
CN108699515A CN201780013255.3A CN201780013255A CN108699515A CN 108699515 A CN108699515 A CN 108699515A CN 201780013255 A CN201780013255 A CN 201780013255A CN 108699515 A CN108699515 A CN 108699515A
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cells
pluripotent stem
derived
endocrine progenitor
human pluripotent
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Chinese (zh)
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N.S.克里斯特弗森
U.多恩
M.汉森
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Novo Nordisk AS
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/845—Gamma amino butyric acid [GABA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN201780013255.3A 2016-02-24 2017-02-24 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞 Pending CN108699515A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16157181 2016-02-24
EP16157181.5 2016-02-24
PCT/EP2017/054390 WO2017144695A1 (en) 2016-02-24 2017-02-24 Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors

Publications (1)

Publication Number Publication Date
CN108699515A true CN108699515A (zh) 2018-10-23

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CN201780013255.3A Pending CN108699515A (zh) 2016-02-24 2017-02-24 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞

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US (1) US11274280B2 (cg-RX-API-DMAC7.html)
EP (1) EP3420073B1 (cg-RX-API-DMAC7.html)
JP (1) JP7123802B2 (cg-RX-API-DMAC7.html)
CN (1) CN108699515A (cg-RX-API-DMAC7.html)
ES (1) ES2967942T3 (cg-RX-API-DMAC7.html)
MA (1) MA45747A (cg-RX-API-DMAC7.html)
WO (1) WO2017144695A1 (cg-RX-API-DMAC7.html)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113069440A (zh) * 2021-04-08 2021-07-06 澳门大学 甲状腺素的新的应用、培养多能干细胞的方法和培养基
CN116286663A (zh) * 2016-06-21 2023-06-23 詹森生物科技公司 人多能干细胞衍生的功能性β细胞的体外产生

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2705001C2 (ru) 2011-12-22 2019-11-01 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека в одногормональные инсулинположительные клетки
AU2014278178B2 (en) 2013-06-11 2021-01-21 President And Fellows Of Harvard College SC-beta cells and compositions and methods for generating the same
SG11201609473XA (en) 2014-05-16 2016-12-29 Janssen Biotech Inc Use of small molecules to enhance mafa expression in pancreatic endocrine cells
CN107614678B (zh) 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
EP3420073B1 (en) 2016-02-24 2023-11-08 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
SG11202001906PA (en) 2017-09-11 2020-04-29 Novo Nordisk As Enrichment of nkx6.1 and c-peptide co-expressing cells derived in vitro from stem cells
WO2019099725A1 (en) 2017-11-15 2019-05-23 Semma Therapeutics, Inc. Islet cell manufacturing compositions and methods of use
WO2019169351A1 (en) 2018-03-02 2019-09-06 Semma Therapeutics, Inc. Methods of enhancing stem cell differentiation into beta cells
EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
AU2018439389A1 (en) * 2018-08-30 2021-02-18 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
EP3953451A1 (en) 2019-04-08 2022-02-16 Novo Nordisk A/S Generation of pancreatic endoderm from stem cell derived definitive endoderm
TW202115245A (zh) 2019-06-27 2021-04-16 丹麥商諾佛 儂迪克股份有限公司 安全免疫隱形細胞
JP2023503302A (ja) 2019-11-22 2023-01-27 ノヴォ ノルディスク アー／エス スピン凝集した神経系マイクロスフェアおよびその応用
WO2021174201A1 (en) * 2020-02-28 2021-09-02 The Trustees Of The University Of Pennsylvania Tissue culture media for survival and engraftment of human pancreatic islets
US20230340595A1 (en) 2020-03-02 2023-10-26 Novo Nordisk A/S Use of pluripotent markers to detect contaminating residual undifferentiated pluripotent stem cells
WO2022043518A1 (en) 2020-08-28 2022-03-03 Novo Nordisk A/S Method for screening in vitro population of stem cell derived beta like cells and novel markers thereof
WO2022129472A1 (en) 2020-12-18 2022-06-23 Novo Nordisk A/S Safe immuno-stealth cells
WO2022136215A1 (en) 2020-12-21 2022-06-30 Novo Nordisk A/S Safe immuno-stealth cells
CA3236375A1 (en) * 2021-10-28 2023-05-04 Lorenz Studer Compositions and methods for promoting in vitro maturation of cells
WO2023118050A1 (en) 2021-12-21 2023-06-29 Novo Nordisk A/S Use of novel markers to detect pluripotent stem cells
WO2024151541A1 (en) 2023-01-09 2024-07-18 Sana Biotechnology, Inc. Type-1 diabetes autoimmune mouse
WO2024243236A2 (en) 2023-05-22 2024-11-28 Sana Biotechnology, Inc. Methods of delivery of islet cells and related methods
WO2025093467A1 (en) 2023-10-31 2025-05-08 Novo Nordisk A/S Methods for preventing unwanted cells arising during differentiation of human pluripotent stem cells

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014201167A1 (en) * 2013-06-11 2014-12-18 President And Fellows Of Harvard College SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME
WO2015028614A1 (en) * 2013-08-30 2015-03-05 Novo Nordisk A/S Generation of endocrine progenitor cells from human pluripotent stem cells using small molecules
CN104411815A (zh) * 2012-04-30 2015-03-11 大学健康网络 用于从hPSC生成胰腺祖细胞和功能性β细胞的方法和组合物
CN104903440A (zh) * 2012-09-03 2015-09-09 诺和诺德股份有限公司 使用小分子从多能干细胞产生胰内胚层
US20150250824A1 (en) * 2014-03-07 2015-09-10 The Research Foundation For The State University Of New York Methods and compositions for expansion of stem cells and other cells

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2516625T3 (da) 2009-12-23 2024-09-09 Janssen Biotech Inc Differentiering af humane embryonale stamceller
AU2011250912A1 (en) 2010-05-12 2012-11-22 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
WO2012175633A1 (en) 2011-06-21 2012-12-27 Novo Nordisk A/S Efficient induction of definitive endoderm from pluripotent stem cells
US9708608B2 (en) * 2012-09-27 2017-07-18 Institut National De La Sante Et De La Recherche Medicale (Inserm) Methods for producing a population of pancreatic beta-cells
MX2015008578A (es) 2012-12-31 2015-09-07 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas en celulas endocrinas pancreaticas mediante el uso de relugadores de hb9.
US20150368616A1 (en) * 2013-02-14 2015-12-24 The Cleveland Clinic Foundation Methods for induction of cell fates from pluripotent cells
CN105531365A (zh) 2013-04-23 2016-04-27 耶达研究及发展有限公司 分离的幼稚多能干细胞和产生所述细胞的方法
WO2014179198A1 (en) 2013-04-29 2014-11-06 Wisconsin Alumni Research Foundation Methods for producing insulin-secreting beta cells from human pluripotent stem cells
SG11201609473XA (en) 2014-05-16 2016-12-29 Janssen Biotech Inc Use of small molecules to enhance mafa expression in pancreatic endocrine cells
EP3420073B1 (en) 2016-02-24 2023-11-08 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
AU2018439389A1 (en) 2018-08-30 2021-02-18 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104411815A (zh) * 2012-04-30 2015-03-11 大学健康网络 用于从hPSC生成胰腺祖细胞和功能性β细胞的方法和组合物
CN104903440A (zh) * 2012-09-03 2015-09-09 诺和诺德股份有限公司 使用小分子从多能干细胞产生胰内胚层
WO2014201167A1 (en) * 2013-06-11 2014-12-18 President And Fellows Of Harvard College SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME
WO2015028614A1 (en) * 2013-08-30 2015-03-05 Novo Nordisk A/S Generation of endocrine progenitor cells from human pluripotent stem cells using small molecules
US20150250824A1 (en) * 2014-03-07 2015-09-10 The Research Foundation For The State University Of New York Methods and compositions for expansion of stem cells and other cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHENG-RAN XU ET AL.: "Dynamics of genomic H3K27me3 domains and role of EZH2 during pancreatic endocrine specification", 《THE EMBO JOURNAL》 *
DONGHUI ZHANG ET AL.: "Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells", 《CELL RESEARCH》 *
FELICIA W. PAGLIUCA ET AL.: "Generation of Functional Human Pancreatic β Cells In Vitro", 《CELL》 *
GUY R. BRISSON, ET AL.: "The Stimulus-Secretion Coupling of Glucose-Induced Insulin Release VII. A PROPOSED SITE OF ACTION FOR ADENOSINE-3",5"-CYCLIC MONOPHOSPHATE", 《JOURNAL OF CLINICAL INVESTIGATION VOLUME》 *
INDRI PURWANA ET AL.: "GABA Promotes Human β-Cell Proliferation and Modulates Glucose Homeostasis", 《DIABETES》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116286663A (zh) * 2016-06-21 2023-06-23 詹森生物科技公司 人多能干细胞衍生的功能性β细胞的体外产生
CN113069440A (zh) * 2021-04-08 2021-07-06 澳门大学 甲状腺素的新的应用、培养多能干细胞的方法和培养基
WO2022213502A1 (zh) * 2021-04-08 2022-10-13 澳门大学 甲状腺素的新的应用、培养多能干细胞的方法和培养基

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ES2967942T3 (es) 2024-05-06
WO2017144695A1 (en) 2017-08-31
EP3420073B1 (en) 2023-11-08
WO2017144695A9 (en) 2017-10-19
US20190085295A1 (en) 2019-03-21
JP2019510482A (ja) 2019-04-18
EP3420073A1 (en) 2019-01-02
MA45747A (fr) 2019-01-02
EP3420073C0 (en) 2023-11-08
US11274280B2 (en) 2022-03-15
JP7123802B2 (ja) 2022-08-23

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