CN108651527A - A kind of plant insecticide preparation method based on root of Stellera chamaejasme - Google Patents
A kind of plant insecticide preparation method based on root of Stellera chamaejasme Download PDFInfo
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- CN108651527A CN108651527A CN201810420036.3A CN201810420036A CN108651527A CN 108651527 A CN108651527 A CN 108651527A CN 201810420036 A CN201810420036 A CN 201810420036A CN 108651527 A CN108651527 A CN 108651527A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The plant insecticide preparation method based on root of Stellera chamaejasme that the invention discloses a kind of.Root-knot nematode is a kind of omnivorousness plant pathogeny line insect of height specialized form.The present invention is specific as follows：One, the Chinese Stellera Root root of drying is crushed.Two, a kind of gained powder is added in ethyl alcohol and is impregnated.Three, ultrasonic wave extraction is carried out three times to soak.Four, extracting solution is carried out being filtered under diminished pressure concentration.Five, extract is added in ethanol water and is dissolved.It obtains extracting preceding liquid.Six, to liquid extractant continuous extraction at room temperature before extraction obtained by step 7, extract liquor is obtained.Extract liquor is carried out to be filtered under diminished pressure concentration.Obtain target extract.Seven, target extract is dried.The present invention improves the extraction rate of Chinese Stellera Root root extract, and extraction can be completed in 13 hours.The present invention has good nematode killing effect.
Description
Technical field
The invention belongs to biological source insecticide technical fields, and in particular to a kind of vegetable source pesticide based on root of Stellera chamaejasme
Agent preparation method.
Background technology
Chinese Stellera Root (Stellera chamaejasme L) refers to Thymelaeceae stellera herbaceos perennial, also known as disconnected
Intestines grass, chervil etc..Chinese Stellera Root be usually grown in height above sea level 2000-4200m on tailo, alpine meadow, be distributed widely in me
State southwest, North China, northeast, the Northwest.Chinese Stellera Root is more toxic and complete stool is toxic, and root toxicity is better than other positions, domestic animal
Poultry, which is easily eaten by mistake, to cause to poison by food.Chinese Stellera Root biomass is big, and population advantage is apparent, is in when being competed with other steppe plants
Advantage easily inhibits herbage growth, is spread without restraint in northern grassland now, herbage is caused to degenerate year after year, caused grassland appearance
Retrogression of succession has jeopardized the development of grassland and animal husbandry.
Root-knot nematode (Meloidogyne) is a kind of omnivorousness plant pathogeny line insect of height specialized form.Known vegetable of causing harm
The nematode of dish mainly has high bow root-knot nematode, peanut root-knot nematode, M hapla, Meloidogyne incognita, Java root knot line
Worm and beet root-knot nematode etc..Nematode host range is extensive, often cause harm melon, solanaceous vegetables, beans and radish, calabash radish, lettuce,
30 various vegetables such as Chinese cabbage, moreover it is possible to propagate some fungies and bacterial disease.Root-knot nematode is mainly caused harm the roots of various vegetables,
It shows as lateral root and fibrous root more normally increases, and form the spherical or conical white root to differ in size on the fibrous root of young root
Tumor, some are in beads shape.Killed strain overground part growth is short and small, slow, leaf color is abnormal, as a result few, low output, or even causes plant
It is dead ahead of time.
Invention content
The plant insecticide preparation method based on root of Stellera chamaejasme that the purpose of the present invention is to provide a kind of.
The present invention is specific as follows：
It no longer changes Step 1: the root of Chinese Stellera Root is taken to be dried to quality under conditions of 30~40 DEG C.
Step 2: being crushed to the Chinese Stellera Root root dried in step 1,300 the polished standard screens is used in combination to be sieved
Choosing.Obtain Chinese Stellera Root root powder.
Step 3: Chinese Stellera Root root powder obtained by step 2 is pressed solid-liquid ratio 1:A, which is added in ethyl alcohol, impregnates 12~24
Hour, 15≤a≤25.Obtain initial ethanol soak.
Step 4: being separated by filtration the initial ethanol soak obtained by step 3 for Chinese Stellera Root root soak and immersion
Ethyl alcohol afterwards.Chinese Stellera Root root soak is pressed into solid-liquid ratio 1 again:B is added to after immersion in ethyl alcohol, and 3≤b≤5 obtain target second
Alcohol soak.
Step 5: being carried out ultrasonic wave three times at a temperature of 35~45 DEG C to targeted ethanol soak obtained by step 4
Extraction.The time of each ultrasonic wave extraction is 30~50min.Chinese Stellera Root root ethanol extract is obtained after filtering.
Step 6: carrying out being filtered under diminished pressure concentration to Chinese Stellera Root root ethanol extract obtained by step 5, ethyl alcohol leaching is obtained
Cream extract.
It is dissolved Step 7: ethanol extract extract obtained by step 6 is added in ethanol water.It obtains extracting preceding liquid.
Step 8: being extracted at room temperature with extractant to liquid before extraction obtained by step 7, extract liquor is obtained.Extract liquor is carried out
It is filtered under diminished pressure concentration.Obtain target extract.
Step 9: target extract is put into baking oven, being dried to quality under conditions of 45~55 DEG C no longer changes.
Obtain target insecticide.
Further, the concentration that is filtered under diminished pressure in step 6 and step 9 is completed by Rotary Evaporators.
Further, in step 8, co-continuous extraction five times, and the extract liquor of five extraction gained is mixed.
Further, in step 2 ethyl alcohol a concentration of 95%.
Further, in step 7 ethanol water a concentration of 30%.
The invention has the advantages that：
1, the present invention improves the extraction rate of Chinese Stellera Root root extract, and extraction can be completed in 13 hours.
2, the present invention has good nematode killing effect.
3, the present invention enables to the harmful Chinese Stellera Root of script to become to generate value, can effectively contain Chinese Stellera Root pair
The damage of grassland environment.
Description of the drawings
Fig. 1 is to carry out nematode in the target insecticide of 8mg/ml concentration and tag the result block diagram of experiment.
Specific implementation mode
Below in conjunction with attached drawing, the invention will be further described.
Step 1: the root of Chinese Stellera Root is taken to be put into baking oven, being dried to quality under conditions of 35 DEG C no longer changes.
Step 2: being crushed to the Chinese Stellera Root root dried in step 1,300 the polished standard screens is used in combination to be sieved
Choosing.Obtain Chinese Stellera Root root powder.
Step 3: Chinese Stellera Root root powder obtained by step 2 is pressed solid-liquid ratio 1:20(g：Ml) it is added to a concentration of 95%
Ethyl alcohol in impregnate 12 hours (a concentration of 95% ethyl alcohol that x grams of root of Stellera chamaejasme portion powder is added to 20x milliliters).It obtains
Initial ethanol soak.
Step 4: being separated by filtration the initial ethanol soak obtained by step 3 for Chinese Stellera Root root soak and immersion
Ethyl alcohol afterwards.Chinese Stellera Root root soak is pressed into solid-liquid ratio 1 again:4 are added to after immersion in ethyl alcohol, obtain targeted ethanol immersion
Liquid.
It is carried Step 5: being carried out ultrasonic wave three times at a temperature of 40 DEG C to targeted ethanol soak obtained by step 4
It takes.The time of each ultrasonic wave extraction is 30min.Chinese Stellera Root root ethanol extract is obtained after filtering.
Step 6: with Rotary Evaporators be filtered under diminished pressure to Chinese Stellera Root root ethanol extract obtained by step 5 dense
Contracting, obtains ethanol extract extract.
It is dissolved Step 7: ethanol extract extract obtained by step 6 is added in a concentration of 30% ethanol water.
It obtains extracting preceding liquid.
Step 8: mixing five extractions with after extractant at room temperature continuous extraction five times to liquid before extraction obtained by step 7
The extract liquor of gained.Extract liquor is carried out with Rotary Evaporators to be filtered under diminished pressure concentration.Obtain target extract.
Step 9: target extract is put into baking oven, being dried to quality under conditions of 50 DEG C no longer changes.Obtain mesh
Mark insecticide.
Extractant is selected according to one in following four embodiments：
Embodiment one,
Extractant uses ethyl alcohol.
Embodiment two,
Extractant uses petroleum ether.
Embodiment three,
Extractant uses chloroform.
Example IV,
Extractant uses ethyl acetate.
Embodiment five,
Extractant uses sterile water.
Caenorhabditis elegans (Caenorhabditis elegans) is a kind of internationally recognized model organism, is that one kind is more
Cell biological, simple in structure, gene sequencing has been completed, and the breeding cycle is short, and culture difficulty is small, can be in laboratory mass propagation.
Compared with other model animals, it is possible to reduce the error caused by individual otherness.Be conducive to carry out bio-toxicity evaluation
Related experiment standardization and normalization, it can also be used to toxicologic study and toxicity assessment.Caenorhabditis elegans has in nematode
Representativeness, target insecticide prepared in accordance with the present invention is to the killing effect of Caenorhabditis elegans it can be seen that target insecticide pair
The killing effect of all kinds of root-knot nematodes.Therefore the present invention carries out killing for target insecticide using Caenorhabditis elegans as subjects
Worm effect test.Specific test procedure is as follows：
The specific method of experiment of tagging is：
1. Caenorhabditis elegans solution is shaken up；Obtain nematode suspension.
2. nematode suspension is implanted sequentially in all a sample holes of five piece of 24 porocyte culture plates；Nematode is outstanding in various kinds hole
The volume of supernatant liquid is V1.Record the quantity of nematode in various kinds hole, V1=100 μ L.
3. the target insecticide that five embodiments obtain is mixed with the medicament mother liquor of dimethyl sulfoxide (DMSO) (DMSO) respectively.And
It is prepared in colorimetric cylinder and obtains five insecticide solution groups.The target desinsection that five insecticide solution groups are obtained with five embodiments
Agent corresponds.Each insecticide solution group includes the insecticide solution of eight kinds of various concentrations.In same insecticide solution group
The concentration of eight kinds of insecticide solutions be followed successively by 0.4mg/ml, 0.8mg/ml, 1.2mg/ml, 2mg/ml, 2.8mg/ml, 4mg/ml,
6mg/ml、8mg/ml。
4. obtained in step 3 five insecticide solution groups are separately added into five 24 porocyte culture plates.24 hole cells
A kind of insecticide solution in the corresponding insecticide solution group of every three sample holes addition in culture plate.(i.e. by 24 sample holes
It is divided into eight groups with three, eight groups of sample holes and eight kinds of insecticide solutions in same insecticide solution group are corresponding respectively).In various kinds hole
The addition of insecticide solution is V2.V2=300 μ L.
5. by the method for step 1 and step 4, with the medicament mother liquor and distilled water of dimethyl sulfoxide (DMSO) respectively as insecticide
Solution repeats 3 groups of experiments (making blank control group using six sample holes of another piece of 24 porocyte culture plates), removes dimethyl
The influence of sulfoxide medicament mother liquor and distilled water to nemic death rate.
6. one piece of 24 porocyte culture plates in five piece of 24 porocyte culture plates and step 5 in step 2 are positioned over temperature
In the incubator that degree is 25 DEG C, humidity is 80%.
7. being put into incubator for 24 hours, after 48h, 72h, record the amount of survival of nematode in various kinds hole respectively.
The virulence regression equation table obtained is as follows：
The results are shown in Figure 1 for the corresponding insect killing effect test of target insecticide of 8mg/ml concentration.
It can be seen that each embodiment has the effect for killing nematode.Wherein, the insecticidal effect of example IV is best.Embodiment
Five insecticidal effect is most weak.
Claims (5)
1. a kind of plant insecticide preparation method based on root of Stellera chamaejasme, it is characterised in that：Step 1: taking Chinese Stellera Root
Root is dried to quality under conditions of 30~40 DEG C and no longer changes；
Step 2: being crushed to the Chinese Stellera Root root dried in step 1,300 the polished standard screens is used in combination to be screened；
To Chinese Stellera Root root powder；
Step 3: Chinese Stellera Root root powder obtained by step 2 is pressed solid-liquid ratio 1:A, which is added in ethyl alcohol, to be impregnated 12~24 hours,
15≤a≤25；Obtain initial ethanol soak；
Step 4: being separated by filtration the initial ethanol soak obtained by step 3 for second after Chinese Stellera Root root soak and immersion
Alcohol；Chinese Stellera Root root soak is pressed into solid-liquid ratio 1 again:B is added to after immersion in ethyl alcohol, 3≤b≤5, obtains targeted ethanol leaching
Steep liquid；
It is carried Step 5: being carried out ultrasonic wave three times at a temperature of 35~45 DEG C to targeted ethanol soak obtained by step 4
It takes；The time of each ultrasonic wave extraction is 30~50min；Chinese Stellera Root root ethanol extract is obtained after filtering；
Step 6: carrying out being filtered under diminished pressure concentration to Chinese Stellera Root root ethanol extract obtained by step 5, ethanol extract extraction is obtained
Take object；
It is dissolved Step 7: ethanol extract extract obtained by step 6 is added in ethanol water；It obtains extracting preceding liquid；
Step 8: being extracted at room temperature with extractant to liquid before extraction obtained by step 7, extract liquor is obtained；Extract liquor is depressurized
Filtering and concentrating；Obtain target extract；
Step 9: target extract is put into baking oven, being dried to quality under conditions of 45~55 DEG C no longer changes；Obtain mesh
Mark insecticide.
2. a kind of plant insecticide preparation method based on root of Stellera chamaejasme according to claim 1, it is characterised in that：
The concentration that is filtered under diminished pressure in step 6 and step 9 is completed by Rotary Evaporators.
3. a kind of plant insecticide preparation method based on root of Stellera chamaejasme according to claim 1, it is characterised in that：
In step 8, co-continuous extraction five times, and the extract liquor of five extraction gained is mixed.
4. a kind of plant insecticide preparation method based on root of Stellera chamaejasme according to claim 1, it is characterised in that：
A concentration of the 95% of ethyl alcohol in step 2.
5. a kind of plant insecticide preparation method based on root of Stellera chamaejasme according to claim 1, it is characterised in that：
A concentration of the 30% of ethanol water in step 7.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810420036.3A CN108651527A (en) | 2018-05-04 | 2018-05-04 | A kind of plant insecticide preparation method based on root of Stellera chamaejasme |
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| CN201810420036.3A CN108651527A (en) | 2018-05-04 | 2018-05-04 | A kind of plant insecticide preparation method based on root of Stellera chamaejasme |
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| CN108651527A true CN108651527A (en) | 2018-10-16 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115053919A (en) * | 2022-07-04 | 2022-09-16 | 北京清源保生物科技有限公司 | Plant source preparation for killing meloidogyne cucurbitae and preparation method thereof |
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2018
- 2018-05-04 CN CN201810420036.3A patent/CN108651527A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115053919A (en) * | 2022-07-04 | 2022-09-16 | 北京清源保生物科技有限公司 | Plant source preparation for killing meloidogyne cucurbitae and preparation method thereof |
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Application publication date: 20181016 |