CN108635325A - Dendrobium stem cell extract anti-aging composition and cosmetic containing composition - Google Patents
Dendrobium stem cell extract anti-aging composition and cosmetic containing composition Download PDFInfo
- Publication number
- CN108635325A CN108635325A CN201810748161.7A CN201810748161A CN108635325A CN 108635325 A CN108635325 A CN 108635325A CN 201810748161 A CN201810748161 A CN 201810748161A CN 108635325 A CN108635325 A CN 108635325A
- Authority
- CN
- China
- Prior art keywords
- extract
- stem cell
- stem
- composition
- noble dendrobium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Biotechnology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses an anti-aging composition of a dendrobium stem cell extract and a cosmetic containing the composition, wherein the anti-aging composition comprises the dendrobium stem cell extract, a bighead atractylodes rhizome extract, lonicera caerulea juice and a turmeric extract; wherein the mass ratio of the dendrobium stem cell extract to the white atractylodes rhizome extract to the blueberries honeysuckle fruit juice to the turmeric extract is (0.5-2.5): (10-20): (5-10). The white atractylodes rhizome extract, the bluefruit honeysuckle juice and the turmeric extract are compounded with the dendrobium stem cell extract to play a role in synergistic synergism of anti-aging, the composition can obviously improve the skin quality, improve the skin elasticity, improve the skin hyperplasia capability and reduce the skin aging process, the effect is obviously higher than that of singly adding one or two extracts, and the composition is safe and has no stimulation.
Description
Technical field
The present invention relates to cosmetic technical field, more particularly to stem of noble dendrobium stem cell extract anti-apolexis composition and containing should
The cosmetics of composition.
Background technology
Wrinkle is skin aging as a result, being that skin lacks moisture, surface fat is reduced, the result of flexibility decrease.Occur
Wrinkle is the mark that bodily fuctions fall into a decline, and in general, if do not paid attention to maintaining, women starts wrinkle increasing after 28 years old
More, the age is bigger, and wrinkle is more.If malnutritive or psychological burden is overweight, wrinkle can also occur in advance.Wrinkle directly affects
The appearance of face.But the tip of the brow is unawares climbed up.Wrinkle, which is the crow's feet at the formidable enemy of beauty, especially canthus, can most show one
Personal aging.
For the different degrees of wrinkle of face, solution face smoothing wrinkle mode now mainly have injecting type face smoothing wrinkle and into
Row operation face smoothing wrinkle two ways.These beauty methods are to sacrifice the holistic health of people to exchange local of short duration beauty effect for
Fruit, this method can not keep the beautiful appearance of a people for a long time, and can make one to generate dependence, and costly, nothing
Method meets the needs of most common feminine halves.
There are many product of anti-wrinkle on the market at present, be largely be chemically synthesized it is obtained, although in a short time can
Enough bring effect, but not solving the problems, such as at all, and phenomena such as excessive chemical products can lead to skin allergy so that skin
Skin problem is more serious.
The stem of noble dendrobium is China's rare traditional Chinese medicine, is put into world's endangered protection plant, 76 kinds known to China, the whole world is about
There are 1500 kinds.《Sheng Nong's herbal classic》Deng record, the stem of noble dendrobium has eliminating impediment, lower gas, tonifying five zang organs consumptive disease, by skin heat symptoms caused by an exopathgen, Qiang Yin
And other effects, it has been investigated that the stem of noble dendrobium can significantly improve superoxide dismutase (SOD) level, lipid peroxide (LPO) is reduced, is adjusted
It is horizontal to save brain monoaminergic transmitters, inhibits similar monoamine oxidase (MAO), plays the role of anti-aging.It received in recent years
The dendrobium polysaccharide of concern also has the multiple efficacies such as significant immune-enhancing activity and anti-aging, radioresistance, such as Tiepi Fengdou
The brilliant, stem of noble dendrobium dissipates, peaceful, the beneficial intestines soup of clearing, noble dendrobium stem distillate, stem of noble dendrobium essence, yin-nourishing oral solution and stem of noble dendrobium piece etc..With dendrobium candidum processing
" maple bucket " or " earrings dendrobium " are referred to as " golden ear dendrobium ", " dendrobium ring ", " Jin Fengdou ", are in great demand in Southeast Asia and America and Europe.
Plant stem cell (plant stem cell) is undifferentiated cell, has very strong self-renewing and persistently divides
Split ability.Studies have shown that the skin care item containing stem cell can stimulate cell turnover, promote collagen secretion, using dry thin
The effect of born of the same parents is more rapidly more effective than general skin care item.Chinese patent CN 102670438B disclose a kind of anti-aging beauty-care shield
Skin product, containing plant stem cell active matter 0.01~3wt% of 0.001~3wt%, Dragosine carnosine, the plant is dry thin
Cytoactive object is PhytocelltecTMMalusDomestica apples stem cell, PhytocelltecTMSolarVitis grapes
Stem cell, Phytocell tecTMAlp Rose rose leafs stem cell, PhytocelltecTM Argan nuts stem cell,
At least one of RegenistemRiceAp red rice stem cells.The cosmetics and skincare product has improvement skin quality, improves skin elasticity
Effect.
Currently, about stem of noble dendrobium stem cell in skin care item, there are no relevant report, therefore, the present invention selects the stem of noble dendrobium dry
Cell is compounded with other plant extract, works out the composition of anti-aging.
Invention content
The object of the present invention is to provide a kind of anti-apolexis composition and purposes containing stem of noble dendrobium stem cell extract,
The preferable anti-aging anti-wrinkle function of the anti-apolexis composition.
To achieve the above object, the technical solution used in the present invention：A kind of anti-apolexis composition, including stem of noble dendrobium stem cell
Extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E；Wherein stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, indigo plant
Fruit honeysuckle fruit juice, Turmeric P.E mass ratio be (0.5~2.5):(10~20):(10~20):(5~10).
Single extract is usually used in the prior art and carries out anti-aging, however its effect is ideal not to the utmost, the application hair
A person of good sense has found by numerous studies, by stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E one
Determine mass ratio to be compounded, anti-aging effects are significantly better than independent extract；And it does not find to use the stem of noble dendrobium in the prior art
Stem cell extract is applied in anti-aging skin care product, and stem of noble dendrobium stem cell has powerful inoxidizability and activity of fighting against senium, can
To increase the concentration of the SOD in human plasma, the ability of oxygen radical is resisted in enhancing, but also can enhance the dense of collagen
Degree, fundamentally the problem of the aging of prevention face.
Preferably, stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be
(1~2.5):(15~20):(10~15):(5~10).
In the present composition, the proportioning of component can influence the effect of composition, the study found that in the preferred proportioning
In range, the antioxidant effect of the present composition is more preferably.
Preferably, stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be
1:15:15:8。
Applicant has been surprisingly found that in many experiments, under the specific quality proportioning, the present composition it is anti-oxidant
Effect reaches best.
Preferably, stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E, mass ratio be
1.5:17:10:5。
Applicant has been surprisingly found that in many experiments, and under the specific weight proportion, the present composition has good
Antioxidant effect.
Preferably, the mass ratio of the Rhizoma Atractylodis Macrocephalae extract and blue fruit honeysuckle fruit juice is 1:1.
Applicant has been surprisingly found that in many experiments, under the specific weight proportion, the present composition it is anti-oxidant
Effect reaches best.
Application of the anti-apolexis composition in preparing cosmetics.
A kind of cosmetics containing anti-apolexis composition.
Preferably, mass percent of the anti-apolexis composition in the cosmetics is 5~10%.
Preferably, mass percent of the anti-apolexis composition in the cosmetics is 6%.
Preferably, further include the component of following mass percents：Thickener 0.1~0.25%, moisturizer 5~15%, breast
Agent 1~4%, preservative 0.1~0.5%, grease 1.5~3.5%, surplus are deionized water.
Antioxidant composition main component and effect used in the present invention are as follows：
The stem of noble dendrobium：It has been investigated that the stem of noble dendrobium can significantly improve superoxide dismutase (SOD) level, lipid peroxide is reduced
(LPO), it is horizontal that brain monoaminergic transmitters are adjusted, inhibits similar monoamine oxidase (MAO), plays the role of anti-aging.Closely
A little dendrobium polysaccharide being concerned in year also have the multiple efficacies such as significant immune-enhancing activity and anti-aging, radioresistance, such as
Capsule Dendrobii, the stem of noble dendrobium dissipate, clearing is peaceful, beneficial intestines soup, noble dendrobium stem distillate, stem of noble dendrobium essence, yin-nourishing oral solution and stem of noble dendrobium piece etc..With dendrobium candidum
" the maple bucket " or " earrings dendrobium " of processing is referred to as " golden ear dendrobium ", " dendrobium ring ", " Jin Fengdou ", is in great demand in Southeast Asia
And it is American-European.
It is raw material that the present invention, which selects the stem cell of the stem of noble dendrobium, and plant stem cell includes about development of plants and to grow all
Formula possesses the cell of eternal life power, is the root (origin) of plant life power.Plant stem cell, which is present in, to be referred to as point
In the special tectonic of raw tissue, there is very surprising power of regeneration.These allow plant constantly to be grown between centuries,
And generate completely new organ.Plant stem cell is the primary raw material and source of skin progenitor cell metabolism, can quickly be activated not
Dormancy stem cell promotes stem cell self-regeneration update, the first viability of reduction epidermal stem cells to keep Skin Cell newborn in a steady stream, from
And reach promotion, firm skin, desalinate the anti-aging effects of wrinkle, while can be easier to be absorbed by the skin the nutrition of plant at
Point；Accelerate human metabolism.
Rhizoma Atractylodis Macrocephalae：Rhizoma Atractylodis Macrocephalae gas faint scent, bitter, it is sweet, warm-natured, enter spleen, stomach, can it is anti-oxidant, slow down aging.Rhizoma Atractylodis Macrocephalae has antioxygen
Change act on, can effective anti-lipid peroxidation, reduce tissue lipid peroxide content, avoid harmful substance to histocyte
The destruction of structure and function, can be to the autoxidation haemolysis of anti-mouse and human red blood cells；Rhizoma Atractylodis Macrocephalae can improve 12 monthly age above mouse
Erythrocyte sod (superoxide dismutase) activity, enhances the effect of scavenging activated oxygen, reduces damage of the oxygen radical to body
Evil, and inhibit the activity of mouse brain monoamine oxidase B；Aged mouse gavage Rhizoma Atractylodis Macrocephalae decocting 4 weeks, is remarkably improved serum SOD-PX
The vigor of (GlutathioneS-transferase Pl), hence it is evident that reduce MDA (malonaldehyde) content in red blood cell；Rhizoma Atractylodis Macrocephalae, which has, delays Aged Mice kidney
The effect of dirty aging can make the Renal Structure of aged mouse have clear improvement；In addition, the also adjustable gastrointestinal motility function of Rhizoma Atractylodis Macrocephalae,
Liver protection, enhancing body's immunity, diuresis.
Blue fruit honeysuckle：With effects for removing toxic heat, there is anti-inflammatory and anti-virus ability, capillary rupture, drop can be prevented
Low blood pressure, the function of detoxification for improving liver, and there is antitumor efficacy, the malaise symptoms after radiotherapy can be alleviated, chemotherapy can be slowed down
The effect that quantity of leucocyte reduces afterwards.Contain a large amount of anthocyanin in blue fruit honeysuckle, there is anti-oxidant and removing ray, and applicant
It was found that blue fruit honeysuckle fruit juice has effects that unexpected anti-aging anti-wrinkle with other extracts compounding of the invention.
Turmeric：Main component is curcumin, turmeric ketone etc., with scavenging hydroxyl, ultra-oxygen anion free radical
Ability can protect collagen and elastin laminin, and protection corium fabric is injury-free, and skin is full of consolidation vigor, Neng Gouyou
The effect of imitating the activity for inhibiting TYR enzyme, playing skin whitening.
The beneficial effects of the present invention are：Stem of noble dendrobium stem cell has powerful inoxidizability and activity of fighting against senium, Ke Yizeng
Add the concentration of the SOD in human plasma, enhancing to resist the ability of oxygen radical, but also the concentration of collagen can be enhanced,
The problem of fundamentally preventing facial aging, while adding with whitening, skin-active, oxygen radical indigo plant fruit honeysuckle fruit can be reduced
Juice, Rhizoma Atractylodis Macrocephalae extract, Turmeric P.E are compounded with stem of noble dendrobium stem cell extract, play the synergy synergistic effect of anti-wrinkle,
Under a variety of extract synergistic effects, product can be obviously improved skin quality, improve skin elasticity, improve the Reproductive activity of skin and reduce
The ager process that declines of skin, it is with obvious effects to add one of which or two kinds of extracts higher than independent and safe and non-stimulating.
Specific implementation mode
Each raw material involved by following embodiment is the commercially available universal product unless otherwise instructed.
The preparation method of stem of noble dendrobium stem cell extract of the present invention is：1) good stem of noble dendrobium leaf is chosen, and is cleaned
Disinfection, and it is cut into last shape, manufacture callus；2) callus is inoculated in solid medium and carries out Fiber differentiation, cut more
For the regeneration site of injured tissue to carry out squamous subculture, the product after culture, which be transferred in fluid nutrient medium, is enlarged training
It supports, transfers to bioreactor and carry out large-scale culture, until obtaining the stem of noble dendrobium stem cell suspension of saturation；3) 2) it will obtain in
Stem of noble dendrobium stem cell suspension is obtained, is filtered with 1 μm of filter, is removed culture medium, harvest the cell of retention；And cell is put into -80 DEG C
Superfreeze 2 hours, then take out defrosting 30 minutes, repeat aforesaid operations 5 times, after last time thaw at RT, with super
Sonication instrument smudge cells collect filtrate, in being concentrated in rotating vacuum evaporator after filter filtering, you can obtain stone
Dry measure used in former times stem cell extract.
The making of above-mentioned callus and cultural method and the squamous subculture used expand culture, extensive training
Foster mode is all made of method well known in the art, is not described in detail herein to obtain the stem cell of saturation.
A kind of anti-apolexis composition, including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, turmeric carry
Take object；The stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be (0.5~
2.5):(10~20):(10~20):(5~10).
The anti-apolexis composition of the present invention can be by by stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice
It is mixed to get by the mass ratio with Turmeric P.E.
The present invention also provides above-mentioned anti-apolexis compositions in cosmetics, cosmetics to include following mass percent
Component, anti-apolexis composition 5~10%, thickener 0.1~0.25%, moisturizer 5~15%, emulsifier 1~4%, preservative
0.1~0.5%, grease 1.5~3.5%, surplus are deionized water；
The thickener is in carbomer, xanthans, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymers
At least one.
The moisturizer is at least one of hydrolysis Sodium Hyaluronate, trehalose, glucan.
The emulsifier is stearine, stereth -2 and the combination of stereth -21, Phosphoric acid monohexadecyl ester
At least one of potassium.
The preservative selects at least one of potassium sorbate, Phenoxyethanol.
The grease is in grape seed oil, Butyrospermum parkii fruit fat, jojoba seed oil, dimethyl silicone polymer, saualane
At least one of.
The preparation method of above-mentioned cosmetics, includes the following steps：
1) moisturizer, deionized water are added in water phase pot, are heated to 85~90 DEG C, stirring and dissolving is water phase；
2) oil phase pot is added in the oiliness material such as the grease, emulsifier, is heated with stirring to 80-85 DEG C, after dispersion completely
It is pumped into emulsification pot, is oil phase；
3) material in water phase pot is slowly pumped into emulsification pot through filter screen and is emulsified, emulsification stops after 3-6 minutes
Homogeneous, open cycle water cooling, is kept stirring and vacuumizes defoaming；
4) 45 DEG C are cooled to, anti-apolexis composition and preservative is added, stirs evenly, when continuing to be cooled to 36 DEG C, discharging,
It gets product.
Embodiment 1
A kind of anti-apolexis composition, including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, turmeric carry
Take object；The stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 1:15:
15:8。
The preparation method of the composition is as described above.
The anti-apolexis composition is used in cosmetics, and cosmetics include the ingredient of following mass percent：Anti-aging combines
Object 5%, carbomer 0.1%, hydrolysis Sodium Hyaluronate 1%, glycerine 10%, trehalose 2%, stearine 3%, sorbic acid
Potassium 0.3%, jojoba seed oil 1.5%, surplus are deionized water.The preparation method of the cosmetics is as described above.
Embodiment 2
A kind of anti-apolexis composition, including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, turmeric carry
Take object；The stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 1.5:
17:10:5。
The anti-apolexis composition is used in cosmetics, and cosmetics include the ingredient of following mass percent：Anti-aging combines
Object 6%, xanthans 0.2%, trehalose 15%, Phosphoric acid monohexadecyl ester potassium 1%, Phenoxyethanol 0.3%, Butyrospermum parkii fruit fat 2%,
Surplus is deionized water.
Embodiment 3
A kind of anti-apolexis composition, including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, turmeric carry
Take object；The stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 2:16:
10:8。
Above-mentioned anti-apolexis composition is used in cosmetics, and cosmetics include the ingredient of following mass percent：Anti-aging group
Close object 8%, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer 0.15%, glucan 10%, 2% stearyl alcohol
Polyethers -2 and the combination of 2% stereth -21, potassium sorbate 0.2%, dimethyl silicone polymer 1.5%, surplus is deionization
Water.
Embodiment 4
A kind of anti-apolexis composition, including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, turmeric carry
Take object；The stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 0.5:
10:20:10。
Above-mentioned composition is used in cosmetics, and cosmetics include the ingredient of following mass percent：Anti-apolexis composition
10%, carbomer 0.15%, glucan 5%, trehalose 10%, Phosphoric acid monohexadecyl ester potassium 3%, potassium sorbate 0.1%, grape pip
Oil 1.5%, dimethyl silicone polymer 1%, saualane 1%, surplus is deionized water.
Embodiment 5
A kind of anti-apolexis composition, including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, turmeric carry
Take object；The stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 2.5:
20:12:10。
Above-mentioned composition is used in cosmetics, and cosmetics include the ingredient of following mass percent：Anti-apolexis composition
8%, xanthans 0.15%, carbomer 0.1%, glucan 5%, Phosphoric acid monohexadecyl ester potassium 1%, stearine 1%, benzene oxygen
Ethyl alcohol 0.3%, potassium sorbate 0.2%, dimethyl silicone polymer 3.5%, surplus are deionized water.
Embodiment 6
A kind of anti-apolexis composition, including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, turmeric carry
Take object；The stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 2.5:
10:10:5。
Above-mentioned composition is used in cosmetics, and cosmetics include the ingredient of following mass percent：Anti-apolexis composition
8%, xanthans 0.2%, glucan 10%, Phosphoric acid monohexadecyl ester potassium 2%, stearine 1%, Phenoxyethanol 0.3%, mountain
Potassium sorbate 0.2%, dimethyl silicone polymer 3.5%, surplus are deionized water.
Embodiment 7
A kind of anti-apolexis composition, including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, turmeric carry
Take object；The stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 0.5:
20:20:10。
Above-mentioned composition is used in cosmetics, and cosmetics include the ingredient of following mass percent：Anti-apolexis composition
6%, carbomer 0.2%, trehalose 10%, Phosphoric acid monohexadecyl ester potassium 3%, Phenoxyethanol 0.4%, dimethyl silicone polymer
3.5%, surplus is deionized water.
Comparative example 1
The anti-apolexis composition formula of this comparative example 1 is：Including Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E,
The Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 15:15:8.
Comparative example 2
The formula of the anti-apolexis composition of this comparative example 2 is：Including stem of noble dendrobium stem cell extract, blue fruit honeysuckle fruit juice, ginger
Yellow extract, the stem of noble dendrobium stem cell extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 1:15:8.
Comparative example 3
The formula of the anti-apolexis composition of this comparative example 3 is：Including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, turmeric
Extract, the stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, Turmeric P.E mass ratio be 1:15:8.
Comparative example 4
The formula of the anti-apolexis composition of this comparative example 4 is：Including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, Lan Guo
Honeysuckle fruit juice, the stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice mass ratio be 1:15:15.
Comparative example 5
The formula of the anti-apolexis composition of this comparative example 5 is：Including blue fruit honeysuckle fruit juice, Turmeric P.E, the indigo plant fruit
Honeysuckle fruit juice, Turmeric P.E mass ratio be 15:8.
Comparative example 6
The formula of the anti-apolexis composition of this comparative example 6 is：Including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, described
Stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract mass ratio be 1:15.
Comparative example 7
The formula of the anti-apolexis composition of this comparative example 7 is：Including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, Lan Guo
Honeysuckle fruit juice, Turmeric P.E, the stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E
Mass ratio is 0.25:15:15:8.
Comparative example 8
The formula of the anti-apolexis composition of this comparative example 8 is：Including stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, Lan Guo
Honeysuckle fruit juice, Turmeric P.E, the stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E
Mass ratio is 1:6:8:8.
The preparation method of composition of comparative example 1~8 is to be mixed to get each ingredient by the mass ratio.
Effect example 1
1~7 senile-resistant efficacy evaluation test of the embodiment of the present invention
Method：
It is prepared respectively with the phosphate buffer (PBS) of 0.2mol/L pH=6.8：
Sample solution 1：1 composition concentration of embodiment is the solution of 0.2mg/ml；
Sample solution 2：2 composition concentration of embodiment is the solution of 0.2mg/ml；
Sample solution 3：3 composition concentration of embodiment is the solution of 0.2mg/ml；
Sample solution 4：4 composition concentration of embodiment is the solution of 0.2mg/ml；
Sample solution 5：5 composition concentration of embodiment is the solution of 0.2mg/ml；
Sample solution 6：6 composition concentration of embodiment is the solution of 0.2mg/ml；
Sample solution 7：7 composition concentration of embodiment is the solution of 0.2mg/ml；
Sample solution 8：1 composition concentration of comparative example is the solution of 0.2mg/ml；
Sample solution 9：2 composition concentration of comparative example is the solution of 0.2mg/ml；
Sample solution 10：3 composition concentration of comparative example is the solution of 0.2mg/ml；
Sample solution 11：4 composition concentration of comparative example is the solution of 0.2mg/ml；
Sample solution 12：5 composition concentration of comparative example is the solution of 0.2mg/ml；
Sample solution 13：6 composition concentration of comparative example is the solution of 0.2mg/ml；
Sample solution 14：7 composition concentration of comparative example is the solution of 0.2mg/ml；
Sample solution 15：8 composition concentration of comparative example is the solution of 0.2mg/ml；
In the sample solution of 0.9ml, the phosphate buffer of 1mlL- tyrosine (0.3mol) and 1ml pH=6.8 is added
(PBS), it is preheated 10 minutes in 37 DEG C of thermostatic water baths, adds 0.1ml tyrosinase aqueous solutions (1.0mg/ml, mcllvains
Buffer solution), it is sufficiently stirred, after being reacted 15 minutes at 37 DEG C, measures 475nm absorbances (D1).In addition, with above-mentioned phosphate buffer
It is similarly reacted instead of style solution, measures absorbance (D2).In order to compare, it is being not added with sample solution and tyrosinase
Under the conditions of, measure absorbance (D3).Inhibitory activity against tyrosinase is calculated with following formula.
Inhibitory activity against tyrosinase (%)=(D2-D1)/(D2-D3) × 100
Above-mentioned prepare liquid is the composition of Examples 1 to 7 and comparative example 1~8.Obtain table 1；
Table 1
As can be seen from Table 1, stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E
Senile-resistant efficacy is all had, wherein both stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract compounding BELAND fruit honeysuckle fruit juice, turmeric carries
Take object compounding effect good, and four to compound its senile-resistant efficacy best.
The quality of stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E in comparative example 7,8
Than not within the scope of the present invention, it can be seen that its effect is not as good as the present invention, it is seen that stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract,
Four kinds of blue fruit honeysuckle fruit juice, Turmeric P.E extracts are combined by weight ratio of the present invention, make cosmetics of the present invention
Antioxidant effect it is more preferable, also, as shown in Table 1, when stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, ginger
The mass ratio of yellow extract is 1:15:15:When 8, the antioxidant effect of cosmetics of the present invention is best, this is that applicant has been surprisingly found that
's.
Effect example 2
Improve wrinkle of skin effect assessment
The composition of the present invention has good oxidation resistant effect.This effect example has investigated Rhizoma Atractylodis Macrocephalae extract and blue fruit honeysuckle
Influence of the mass ratio of fruit juice to present composition anti-aging effects.In order to investigate the influence, we according to embodiment 1 side
Method is prepared for different compositions.Each experimental group composition includes stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit
Juice, Turmeric P.E, the stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio
For (0.5~2.5):(10~20):(10~20):(5~10)；Wherein the quality of Rhizoma Atractylodis Macrocephalae extract and blue fruit honeysuckle fruit juice is such as
Shown in the following table 2.
Table 2
Test method：
Experimental subjects：Choose the healthy volunteer 20 for there are the wrinkles such as dry line, microgroove, crow's feet, expression line, neck line to perplex
Name,
Man, each 10 of female, age 38-41 Sui, no serious systemic disease, without immune deficiency or autoimmune disease, nothing
Activity anaphylactia, to skin-care cosmetics without severe allergy history, it is 1 month nearly in used hormone medicine or immune
Inhibitor does not participate in other clinical tests, does not smear the external preparations such as any cosmetics.4 groups are randomly divided into, every group of 5 people.
After each group volunteer warm water cleaning skin, phase is uniformly smeared in face, eye circumference, neck, the back of the hand (same position)
The cosmetics answered, daily each 1 time of morning and evening after being used continuously 8 weeks, use moisture of skin measuring instrument Corneometer CM825 to survey
The water content (%) in skin of dorsum of hand cuticula is measured, scattering and disappearing using transepidermal water, to measure skin of dorsum of hand water-dispersed by analyzer TM300
Vector [TEML, g/ (hm2)]。
Anti-wrinkle efficacy assessments are carried out using wrinkle of skin tester SV600, silicon mould is first used to replicate the skin of institute's measurement site
Wrinkle model, computer carry out grade separation and statistical disposition according to wrinkle of skin depth.Before calculating sample use, after use
Skin mean roughness R3 (takes arithmetic mean number, wherein R1 to indicate the R1 values measured by 5 equal lengths, different segment
In certain length peak value between minimum at a distance from, μm).As a result Δ R3 is used (i.e.：Using before sample with use sample after
The difference of skin mean roughness) it indicates, numerical value is higher, and it is better to represent effect improving wrinkles.
Facial skin elasticity (it is 450mpa that negative pressure is constant in test process) is tested using skin elasticity tester MPA580,
As a result the ratio of springback capacity Ur (recovery value for cancelling negative pressure skin after 0.1 second) and the maximum tension amount Uf of skin is used to indicate,
Numerical value illustrates that skin elasticity is better closer to 1.
As a result table 3 is obtained：
Table 3
As seen from Table 3, the mass ratio of Rhizoma Atractylodis Macrocephalae extract and blue fruit honeysuckle fruit juice is 1:1, anti-aging effects are best.Change
Kind skin hydration degree reduces water loss amount, improves skin elasticity and wrinkle and other effects most preferably.
Guinea pig skin stimulation test
0.5g Examples 1 to 7 anti-apolexis compositions are respectively taken, being diluted to 100g using deionized water is configured to given the test agent.
Given the test agent using skin irritation test method (《Cosmetics health specification》2007 editions toxicology test methods) it is tested.
Experimental animal：Cavy weight 250g~300g.
Experimental result：Through 14 days Skin Irritation Tests, stimulate the reaction is had no.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is protected to the present invention
The limitation of range is protected, although being explained in detail to the present invention with reference to preferred embodiment, those skilled in the art should
Understand, technical scheme of the present invention can be modified or replaced equivalently, without departing from the essence of technical solution of the present invention
And range.
Claims (10)
1. a kind of anti-apolexis composition, which is characterized in that include stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit
Juice, Turmeric P.E；The mass ratio of the stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae extract, blue fruit honeysuckle fruit juice, Turmeric P.E
For (0.5~2.5):(10~20):(10~20):(5~10).
2. anti-apolexis composition according to claim 1, which is characterized in that the stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae carry
Take object, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be (1~2.5):(15~20):(10~15):(5~10).
3. anti-apolexis composition according to claim 1, which is characterized in that the stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae carry
Take object, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 1:15:15:8.
4. anti-apolexis composition according to claim 1, which is characterized in that the stem of noble dendrobium stem cell extract, Rhizoma Atractylodis Macrocephalae carry
Take object, blue fruit honeysuckle fruit juice, Turmeric P.E mass ratio be 1.5:17:10:5.
5. anti-apolexis composition according to claim 1, which is characterized in that the Rhizoma Atractylodis Macrocephalae extract and blue fruit honeysuckle fruit juice
Mass ratio be 1:1.
6. according to application of any one of Claims 1 to 5 anti-apolexis composition in preparing cosmetics.
7. a kind of cosmetics containing the anti-apolexis composition as described in any one of Claims 1 to 5.
8. cosmetics according to claim 7, which is characterized in that matter of the anti-apolexis composition in the cosmetics
It is 5~10% to measure percentage.
9. cosmetics according to claim 7, which is characterized in that matter of the anti-apolexis composition in the cosmetics
It is 6% to measure percentage.
10. cosmetics according to claim 8, it is characterised in that：It further include the ingredient of following mass percents：Thickener
0.1~0.25%, moisturizer 5~15%, emulsifier 1~4%, preservative 0.1~0.5%, grease 1.5~3.5%, surplus are
Deionized water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810748161.7A CN108635325A (en) | 2018-07-09 | 2018-07-09 | Dendrobium stem cell extract anti-aging composition and cosmetic containing composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810748161.7A CN108635325A (en) | 2018-07-09 | 2018-07-09 | Dendrobium stem cell extract anti-aging composition and cosmetic containing composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108635325A true CN108635325A (en) | 2018-10-12 |
Family
ID=63751332
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810748161.7A Withdrawn CN108635325A (en) | 2018-07-09 | 2018-07-09 | Dendrobium stem cell extract anti-aging composition and cosmetic containing composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108635325A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110840807A (en) * | 2019-12-14 | 2020-02-28 | 广州尚妆优品生物科技有限公司 | Wrinkle-removing composition |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
-
2018
- 2018-07-09 CN CN201810748161.7A patent/CN108635325A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
Non-Patent Citations (1)
| Title |
|---|
| 王建新等: "《化妆品植物原料大全》", 30 June 2012 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110840807A (en) * | 2019-12-14 | 2020-02-28 | 广州尚妆优品生物科技有限公司 | Wrinkle-removing composition |
| CN110840807B (en) * | 2019-12-14 | 2022-06-07 | 广州尚妆优品生物科技有限公司 | Wrinkle-removing composition |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2021020887A (en) | Enhanced anti-aging cosmetic composition | |
| CN104644523B (en) | A kind of medical bio smoothing wrinkle suppression spot dressing and preparation method thereof | |
| KR101762594B1 (en) | Composition for curing skin using freshwater cancellous bone grafts and manufacturing method thereof | |
| TWI693899B (en) | Fermentation product of punica granatum and uses thereof | |
| KR101059471B1 (en) | Cosmetic composition for skin aging | |
| CN108670943A (en) | A kind of moisturizing, whitening, light spot composition of plant extracts | |
| CN103237555A (en) | Topical skin care formulation | |
| KR101425031B1 (en) | Cosmetic composition for anti-irritation and skin moisturization containing Citrus junos Siebold seed oil and Mangifera Indica seed oil | |
| EA021777B1 (en) | Composition containing extract of venus flytrap dionaea muscipula for cosmetic treatment of the skin and skin appendages | |
| KR101352363B1 (en) | Composition of skin external application for moisturizing comprising Scrophularia buergeriana Miq. extract | |
| KR101825704B1 (en) | A cosmetic composition comprising coffee silver skin extract | |
| US20150182443A1 (en) | Traditional chinese medicine extract composition with whitening skin-care function adn its preparation method | |
| CN108635326A (en) | Anti-aging and wrinkle-removing composition containing dendrobium stem cell extract and application thereof | |
| KR102101949B1 (en) | A skin-care agent or cosmetics containig Antrodia camphorata fruit body extract or Antrodia camphorata mycelium extract | |
| KR101841118B1 (en) | Composition for skin external application comprising extract of scenedesmus sp. | |
| TW201420128A (en) | Extract of Phalaenopsis amabilis embryo, and the preparation process and uses thereof | |
| CN108635325A (en) | Dendrobium stem cell extract anti-aging composition and cosmetic containing composition | |
| KR20110032715A (en) | Antiaging cosmetic compositions and method for producing thereof | |
| KR102171924B1 (en) | External skin preparation for relaxing allergy comprising extract of wild-peach and method of producing the same | |
| CN108635327A (en) | Dendrobium stem cell extract anti-aging composition and cosmetic containing composition | |
| CN113876671A (en) | Anti-aging composition containing rape bud extract and preparation method thereof | |
| KR102073943B1 (en) | A skin-care agent or cosmetics containig Lyophyllum ulmarium fruit body extract or Lyophyllum ulmarium mycelium extract | |
| CN108567732A (en) | Anti-aging composition containing dendrobium stem cell extract and preparation method thereof | |
| KR101872353B1 (en) | Cosmetic composition comprising the very high-pressure extract of narcissus tazetta bulb fermentation | |
| KR100946584B1 (en) | Cosmetic Composition Comprising Poncirus trifoliata fruit extract and Nymphaea caerulea extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181012 |
|
| WW01 | Invention patent application withdrawn after publication |