CN108633928A - One Plant Extracts sterilization bacteriostat stoste and preparation method thereof - Google Patents
One Plant Extracts sterilization bacteriostat stoste and preparation method thereof Download PDFInfo
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- CN108633928A CN108633928A CN201810374317.XA CN201810374317A CN108633928A CN 108633928 A CN108633928 A CN 108633928A CN 201810374317 A CN201810374317 A CN 201810374317A CN 108633928 A CN108633928 A CN 108633928A
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- plant extracts
- stoste
- sterilization bacteriostat
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/18—Euphorbiaceae [Spurge family], e.g. ricinus [castorbean]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/30—Polygonaceae [Buckwheat family], e.g. red-knees or rhubarb
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
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- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention is suitable for biological antibiotic field, one Plant Extracts sterilization bacteriostat and preparation method thereof is provided, with gleditsia sinensis, rheum officinale, asparagus fern, Rhizoma Atractylodis Macrocephalae, radix euphorbiae lantu, numb seed, aloe is to prepare plant sterilization bacteriostatic agent primary raw material, the active component of several plants of the optimum option with bactericidal bacteriostasis, extraction wherein active ingredient, water base sterilization bacteriostat is prepared out using pure bio-extract, it can be with direct body contact, it will not be to people and animals, environment generates any harm, the biological raw material that the present invention uses is simple and easy to get, it prepares convenient and simple, it is easy to popularization and application, staphylococcus aureus can effectively be killed, Candida albicans, Escherichia coli, the bacteriums such as pathogenic pyogenic baeceria.
Description
Technical field
The invention belongs to biological antibiotic field more particularly to a Plant Extracts sterilization bacteriostats.
Background technology
Sterilization bacteriostat currently on the market is mostly that chemicals mixes, therefore has toxic side effect, and chemistry is residual
Staying can be to environmental danger.Many sterilization bacteriostats also contain the inflammable and explosive ingredients such as ethyl alcohol, cause security risk.Some are killed
Bacterium bacteriostatic agent contains peroxide, has strong corrosivity, easily causes secondary harm.
Invention content
In view of the above problems, the purpose of the present invention is to provide a Plant Extracts sterilization bacteriostat stoste and its preparations
Method, it is intended to solve existing sterilization bacteriostat using there are the not strong technologies of security risk, strong toxicity, bactericidal and bacteriostatic effect to ask
Topic.
The preparation method of one Plant Extracts sterilization bacteriostat stoste, the method includes following step：
A, weigh according to a certain percentage gleditsia sinensis, rheum officinale, asparagus fern, Rhizoma Atractylodis Macrocephalae, radix euphorbiae lantu, numb seed, aloe dried plant raw material, powder
Breakdown mill is mixed to get powdered plants, and obtained powdered plants are put into heating container, and water then is added into heating container,
Process by immersion, heating, cooling, filtering obtains yellowish liquid；
B, it takes dry antibody source attrition grinding to obtain antibody source powder, a round is taken to be put into nutrient source, be added
A certain amount of afore mentioned antibodies source powder and water simultaneously stir, and so that mixture is become mixing body of paste, then be added into mixing paste body
Antibody lures source, lasting to stir, it is to be mixed uniformly after mixture is heated, keep certain temperature and humidity, finally will mixing
Object dries to obtain ferment；
C, the yellowish liquid of step a is taken to be mixed according to certain mass ratio with the ferment of step b, in room temperature or heating
Under the conditions of stirring until all dissolving, obtain sterilization bacteriostat stoste.
The present invention also provides a Plant Extracts sterilization bacteriostat stoste, the plant extracts sterilization bacteriostat stostes
It is prepared by the preparation method of the Plant Extracts sterilization bacteriostat stoste.
The present invention also provides a Plant Extracts sterilization bacteriostat, the plant extracts sterilization bacteriostat is by the plant
Object extract sterilization bacteriostat stoste is with water according to mass ratio 1:(20~200) are mixed to get.
The present invention is mainly former to prepare plant sterilization bacteriostatic agent with gleditsia sinensis, rheum officinale, asparagus fern, Rhizoma Atractylodis Macrocephalae, radix euphorbiae lantu, numb seed, aloe
Material, the active component of several plants of the optimum option with bactericidal bacteriostasis, extraction wherein active ingredient, using pure biology
Extract prepares out water base sterilization bacteriostat, can will not generate any harm to people and animals, environment with direct body contact, this
It is simple and easy to get to invent the biological raw material used, prepares convenient and simple, is easy to popularization and application, can effectively kill Staphylococcus aureus
The bacteriums such as bacterium, Candida albicans, Escherichia coli, pathogenic pyogenic baeceria.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The preparation method of one Plant Extracts sterilization bacteriostat stoste, includes the following steps：
A, weigh according to a certain percentage gleditsia sinensis, rheum officinale, asparagus fern, Rhizoma Atractylodis Macrocephalae, radix euphorbiae lantu, numb seed, aloe dried plant raw material, powder
Breakdown mill is mixed to get powdered plants, and obtained powdered plants are put into heating container, and water then is added into heating container,
Process by immersion, heating, cooling, filtering obtains yellowish liquid.
It is heated in this step after water is added in container, it is as follows to heat each component mass ratio in container：It is gleditsia sinensis 5%~7%, big
Huang 2%~4%, asparagus fern 5%~7%, Rhizoma Atractylodis Macrocephalae 5%~7%, radix euphorbiae lantu 5%~7%, numb seed 3%~5%, aloe 5%~8%,
Surplus is water.Soaking time is 15~60 minutes, and heating temperature is 50~60 DEG C, 30~240 minutes postcoolings of heat preservation to room temperature.
B, it takes dry antibody source attrition grinding to obtain antibody source powder, a round is taken to be put into nutrient source, be added
A certain amount of afore mentioned antibodies source powder and water simultaneously stir, and so that mixture is become mixing body of paste, then be added into mixing paste body
Antibody lures source, lasting to stir, it is to be mixed uniformly after mixture is heated, keep certain temperature and humidity, finally will mixing
Object dries to obtain ferment.
Antibody source described in this step is gleditsia sinensis, rheum officinale, asparagus fern, Rhizoma Atractylodis Macrocephalae, the one or more mixtures of aloe, the nutrition
Source is starch, and it is strain that the antibody, which lures source,.Antibody source powder and starch are according to mass ratio (0.5~45)：100 mixing, strain
Quality be mix paste body mass ratio ten a ten thousandths to one thousandth, heating condition is to be gradually heated to 40~85 DEG C,
For humidity when heat preservation between relative humidity 50~95%, soaking time is 24~48 hours.
C, the yellowish liquid of step a is taken to be mixed according to certain mass ratio with the ferment of step b, in room temperature or heating
Under the conditions of stirring until all dissolving, obtain sterilization bacteriostat stoste.
Yellowish liquid described in this step is with ferment according to mass ratio 1000:(0.2~1.8) it mixes.Heating temperature can
It it is 40~75 DEG C, mixing time is 30~60 minutes.
By above-mentioned plant extracts sterilization bacteriostat stoste and water according to mass ratio 1:(20~200) are mixed to get a plant
Object extract sterilization bacteriostat.
Gleditsia sinensis B-G containing gleditschiasaponin (gleditsiasaaponin B-G), palmitic acid (palmitic acid), hard ester
Acid, oleic acid, sub- sterol, sitosterol, nonacosane (nonacosane), can subside a swelling eliminating toxic, apocenosis, desinsection.For at the beginning of ulcer
It rises or purulenceization is not burst.Rheum officinale contains anthraquinone and rheum emodin, have under rushing down, be antibacterial, hemostasis the effects that.Asparagus fern contains a variety of spiral steroid glucosides
Close nearly 20 kinds of amino acid and the oligosaccharide I such as object asparagus fern glucoside-IV~VII (Asp- IV~VII), asparagine, citrulling, serine
~VII, and contain 5-methoxy-methylfurfural (5-methoxy-methylfurfural), there are mosquitocidal fly and bacteriostasis.In vain
Art contains volatile oil, inside there is α-and β-Humuleno (humu-lene), β-elemol (β-elemol), α curcumenes (α-
Curcumene) etc., there is bacteriostasis.Radix euphorbiae lantu has sterilization infanticide Jue effects containing sterol, phenolic constituent, amino acid, triterpenes.Fiber crops
Seed contains linoleic acid and linolenic acid, there is lubrication osmosis.Aloe Aloin A containing isocitric acid, wound hormone and glycan peptide are sweet
Dew polysaccharide and multivitamin have anti-inflammation and sterilization, heat absorption detumescence, softening skin, keep cell viability function, curdlan and
Traumatic acid joint also has healing wounds activity.The present invention passes through the formulation optimization of above-mentioned plant extracts, more than rational proportion
Various plant live parts, are made the aqueous solution containing active ingredient, play excellent bactericidal bacteriostasis, are extracted using pure biology
Object prepares out water base sterilization bacteriostat, can will not generate any harm to people and animals, environment with direct body contact.
In order to illustrate technical solutions according to the invention, illustrated below by specific embodiment.
5 parts of gleditsia sinensis, 3 parts of rheum officinale, 5 parts of asparagus fern, 5 parts of Rhizoma Atractylodis Macrocephalae, 5 parts of radix euphorbiae lantu, numb 3 parts of seed, aloe 6 are weighed by weight percentage
Part, attrition grinding is mixed to get powdered plants, obtained powdered plants is put into heatable container, then adds to heating container
Enter pure water or drinkable tap water to 100 parts, first impregnate 30 minutes in water, be then heated to 55 DEG C, is kept for 60 minutes, it is cold
But to room temperature, yellowish liquid is obtained by filtration.
Each 2 parts of dry gleditsia sinensis, asparagus fern, aloe are taken, attrition grinding obtains antibody source powder, is put into a round
Antibody source powder and 94 parts of starch, stir evenly, and being put into a small amount of water makes mixture become paste, adds 0.02 part of bacterium
Kind, it is uniformly mixed, is gradually heated to 60 DEG C and keeps temperature, keep humidity between relative humidity 90%, kept for 24 hours, obtained
To ferment, harvest drying is for use.
Take yellowish liquid with ferment according to weight ratio 1000:1 mixing is stirred at room temperature until all dissolvings, continue
Stirring 30 minutes, obtains sterilization bacteriostat stoste, by sterilization bacteriostat stoste according to 1:30 dilutions, take 5ml dilutions as real
Apply the sample of example.
Experiment one：Bacteriostatic test
Bacteriostatic test is carried out after 100 μ l bacteria suspensions of addition are mixed to the sample of embodiment and acts on different time respectively：
2min, 5min, 10min, 20min, inoculated and cultured, 37 DEG C of constant temperature incubation 48h carry out bacterium colony counting, calculate bacteriostasis rate, experiment weight
Again three times, concrete outcome is as shown in the table.
Staphylococcus aureus bacteriostatic test result
Positive controls clump count and range 4.77*104cfu/ml(4.40*104Cfu/ml~5.10*104cfu/ml)
Negative control group asepsis growth
Candida albicans bacteriostatic test result
Positive controls clump count and range 2.07*104cfu/ml(1.86*104Cfu/ml~2.25*104cfu/ml)
Negative control group asepsis growth
Escherichia coli bacteriostatic test result
Positive controls clump count and range 5.07*104cfu/ml(4.50*104Cfu/ml~6.10*104cfu/ml)
Negative control group asepsis growth
As can be seen from the table, through three times repeat experiment results proved, 1:The dilution of 30 sterilization bacteriostat stostes acts on
Different time different strain bacteriostasis rate average out to 100%, it is known that the bactericidal effect of this sterilization bacteriostat is good, and antibacterial type is wide
It is general.
Experiment two：Skin irritatin is tested
Skin irritatin testing experiment is carried out to the sample of embodiment.The 3 female big ear white race rabbit of regular grade, weight are taken to be
2.4~2.6kg.Animal feeding environment：Room temperature is (18~26) DEG C, relative humidity (40~70) %RH, single cage raising.
Method：Daily every animal dosage is 0.5ml, and the healthy rabbits 3 for selecting skin intact for 24 hours will before experiment
Its back backbone both sides hair is cut, and the left and right each about 3cm*3cm of gross area is removed.The sample 0.5ml of Example is coated in when experiment
On the skin of side, as a contrast, the 4h after smearing is cleaned other side physiological saline with clear water, is removed residue, is smeared one daily
Secondary, unhairing before smearing can not damage epidermis, continuously smear 14d.Result is observed for 24 hours after smearing every time.Concrete outcome such as following table institute
Show：
As can be seen from the table, in experimental period, the tested side skin of animal subject has no obvious stimulation symptom, has no tested dynamic
There are other signs of toxicity in object.Situations such as without erythema, oedema.Tested material is 0 to experimental animal skin wound repair integral mean value,
According to the Ministry of Public Health《Disinfection technology standard》Skin irritatin strength grading standard determination in (version in 2002), this test specimen is to rabbit
Multiple intact skin irritant test result is nonirritant.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (9)
1. the preparation method of a Plant Extracts sterilization bacteriostat stoste, which is characterized in that the method includes following step：
A, weigh according to a certain percentage gleditsia sinensis, rheum officinale, asparagus fern, Rhizoma Atractylodis Macrocephalae, radix euphorbiae lantu, numb seed, aloe dried plant raw material, crushing grinds
Mill is mixed to get powdered plants, and obtained powdered plants are put into heating container, and water then is added into heating container, passes through
It impregnates, heat, is cooling, filtering process obtains yellowish liquid；
B, it takes dry antibody source attrition grinding to obtain antibody source powder, a round is taken to be put into nutrient source, be added certain
The afore mentioned antibodies source powder and water of amount simultaneously stir, and so that mixture is become mixing body of paste, then antibody is added into mixing paste body
Lure source, it is lasting to stir, it is to be mixed uniformly after mixture is heated, keep certain temperature and humidity, finally dry mixture
It is dry to obtain ferment；
C, the yellowish liquid of step a is taken to be mixed according to certain mass ratio with the ferment of step b, in room temperature or the condition of heating
Lower stirring obtains sterilization bacteriostat stoste until all dissolvings.
2. the preparation method of Plant Extracts sterilization bacteriostat stoste as described in claim 1, which is characterized in that step a
Heating container in be added water after, heat container in each component mass ratio it is as follows：Gleditsia sinensis 5%~7%, rheum officinale 2%~4%, day
Winter 5%~7%, Rhizoma Atractylodis Macrocephalae 5%~7%, radix euphorbiae lantu 5%~7%, numb seed 3%~5%, aloe 5%~8%, surplus is water.
3. the preparation method of Plant Extracts sterilization bacteriostat stoste as claimed in claim 2, which is characterized in that step a
In, soaking time is 15~60 minutes, and heating temperature is 50~60 DEG C, 30~240 minutes postcoolings of heat preservation to room temperature.
4. the preparation method of Plant Extracts sterilization bacteriostat stoste as described in claim 1, which is characterized in that step b
In, the antibody source is gleditsia sinensis, rheum officinale, asparagus fern, Rhizoma Atractylodis Macrocephalae, the one or more mixtures of aloe, and the nutrient source is starch, described
It is strain that antibody, which lures source,.
5. the preparation method of Plant Extracts sterilization bacteriostat stoste as claimed in claim 4, which is characterized in that step b
In antibody source powder and starch according to mass ratio (0.5~45)：The quality of 100 mixing, strain is mixing paste body mass ratio
Ten a ten thousandths to one thousandth, heating condition is to be gradually heated to 40~85 DEG C, and humidity when heat preservation is relative humidity 50
Between~95%, soaking time is 24~48 hours.
6. the preparation method of Plant Extracts sterilization bacteriostat stoste as described in claim 1, which is characterized in that step c
In, the yellowish liquid is with ferment according to mass ratio 1000:(0.2~1.8) it mixes.
7. the preparation method of Plant Extracts sterilization bacteriostat stoste as claimed in claim 6, which is characterized in that step c
In, heating temperature is 40~75 DEG C, and mixing time is 30~60 minutes.
8. a Plant Extracts sterilization bacteriostat stoste, which is characterized in that the plant extracts sterilization bacteriostat stoste by
Any one of claim 1-7 the methods are prepared.
9. a Plant Extracts sterilization bacteriostat, which is characterized in that the plant extracts sterilization bacteriostat is wanted by such as right
Ask the plant extracts sterilization bacteriostat stoste described in 8 with water according to mass ratio 1:(20~200) are mixed to get.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113170799A (en) * | 2021-05-13 | 2021-07-27 | 烟台市步鹤山农业科技有限公司 | Traditional Chinese medicine enzyme mixed pesticide for preventing and treating diseases and insect pests of tea garden |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113170799A (en) * | 2021-05-13 | 2021-07-27 | 烟台市步鹤山农业科技有限公司 | Traditional Chinese medicine enzyme mixed pesticide for preventing and treating diseases and insect pests of tea garden |
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