CN108624637B - 一种利用大米生产海藻糖的方法 - Google Patents
一种利用大米生产海藻糖的方法 Download PDFInfo
- Publication number
- CN108624637B CN108624637B CN201810970927.6A CN201810970927A CN108624637B CN 108624637 B CN108624637 B CN 108624637B CN 201810970927 A CN201810970927 A CN 201810970927A CN 108624637 B CN108624637 B CN 108624637B
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- China
- Prior art keywords
- trehalose
- rice
- cooling
- hours
- sugar
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 title claims abstract description 83
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 title claims abstract description 83
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 title claims abstract description 79
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 47
- 235000009566 rice Nutrition 0.000 title claims abstract description 47
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 15
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 46
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- 108010065511 Amylases Proteins 0.000 claims abstract description 15
- 102000013142 Amylases Human genes 0.000 claims abstract description 15
- 102000004157 Hydrolases Human genes 0.000 claims abstract description 15
- 108090000604 Hydrolases Proteins 0.000 claims abstract description 15
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 15
- 235000019418 amylase Nutrition 0.000 claims abstract description 15
- 239000004382 Amylase Substances 0.000 claims abstract description 14
- 108010039327 maltooligosyl trehalose synthase Proteins 0.000 claims abstract description 10
- 238000001816 cooling Methods 0.000 claims description 50
- 239000000463 material Substances 0.000 claims description 36
- 235000020195 rice milk Nutrition 0.000 claims description 36
- 239000000706 filtrate Substances 0.000 claims description 30
- 238000001914 filtration Methods 0.000 claims description 23
- 238000002425 crystallisation Methods 0.000 claims description 17
- 230000008025 crystallization Effects 0.000 claims description 17
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 12
- 239000013078 crystal Substances 0.000 claims description 12
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 10
- 102000004169 proteins and genes Human genes 0.000 claims description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 239000003513 alkali Substances 0.000 claims description 9
- 238000000227 grinding Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 229910052740 iodine Inorganic materials 0.000 claims description 7
- 239000011630 iodine Substances 0.000 claims description 7
- 238000005070 sampling Methods 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 7
- 230000000415 inactivating effect Effects 0.000 claims description 6
- 239000003957 anion exchange resin Substances 0.000 claims description 5
- 239000003729 cation exchange resin Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 19
- 239000000758 substrate Substances 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 39
- 229940088598 enzyme Drugs 0.000 description 23
- 238000001514 detection method Methods 0.000 description 11
- 239000000047 product Substances 0.000 description 7
- 239000011347 resin Substances 0.000 description 6
- 229920005989 resin Polymers 0.000 description 6
- 102000003960 Ligases Human genes 0.000 description 5
- 108090000364 Ligases Proteins 0.000 description 5
- 239000003456 ion exchange resin Substances 0.000 description 5
- 229920003303 ion-exchange polymer Polymers 0.000 description 5
- 239000000126 substance Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical group CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 150000001450 anions Chemical class 0.000 description 3
- 150000001768 cations Chemical class 0.000 description 3
- 239000012295 chemical reaction liquid Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 239000012982 microporous membrane Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 229940100486 rice starch Drugs 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102100022624 Glucoamylase Human genes 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- AYRXSINWFIIFAE-SCLMCMATSA-N Isomaltose Natural products OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@@H](O)[C@@H](O)[C@@H]1O AYRXSINWFIIFAE-SCLMCMATSA-N 0.000 description 1
- 229920001218 Pullulan Polymers 0.000 description 1
- 239000004373 Pullulan Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- OCIBBXPLUVYKCH-QXVNYKTNSA-N alpha-maltohexaose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](O[C@H](O[C@@H]3[C@H](O[C@H](O[C@@H]4[C@H](O[C@H](O[C@@H]5[C@H](O[C@H](O)[C@H](O)[C@H]5O)CO)[C@H](O)[C@H]4O)CO)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O OCIBBXPLUVYKCH-QXVNYKTNSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- DJMVHSOAUQHPSN-UHFFFAOYSA-N malto-hexaose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(OC4C(C(O)C(O)C(CO)O4)O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 DJMVHSOAUQHPSN-UHFFFAOYSA-N 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002840 non-reducing disaccharides Chemical class 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 235000019423 pullulan Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 108010045348 trehalose synthase Proteins 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
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CN201810970927.6A CN108624637B (zh) | 2018-08-24 | 2018-08-24 | 一种利用大米生产海藻糖的方法 |
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CN201810970927.6A CN108624637B (zh) | 2018-08-24 | 2018-08-24 | 一种利用大米生产海藻糖的方法 |
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CN108624637A CN108624637A (zh) | 2018-10-09 |
CN108624637B true CN108624637B (zh) | 2021-10-19 |
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CN201810970927.6A Active CN108624637B (zh) | 2018-08-24 | 2018-08-24 | 一种利用大米生产海藻糖的方法 |
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CN112941130A (zh) * | 2021-04-20 | 2021-06-11 | 江苏省奥谷生物科技有限公司 | 一种多酶复配生产海藻糖的方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1085096A2 (en) * | 1993-07-20 | 2001-03-21 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Preparation of high trehalose content product |
CN102747120A (zh) * | 2012-07-18 | 2012-10-24 | 庄晟 | 一种月饼专用转化糖浆的制备方法 |
CN103946386A (zh) * | 2011-09-21 | 2014-07-23 | 株式会社林原 | 含有α,α-海藻糖二水合物晶体的粉末的制备方法 |
CN105925642A (zh) * | 2016-06-14 | 2016-09-07 | 湖南汇升生物科技有限公司 | 用微生物发酵法工业化生产海藻糖的方法 |
KR20170024993A (ko) * | 2015-08-27 | 2017-03-08 | (주)무성 | 밥류 및 떡류의 전분 노화억제용 조성물 및 그 제조방법 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102965412A (zh) * | 2012-12-13 | 2013-03-13 | 湖南润涛生物科技有限公司 | 一种海藻糖的制备方法 |
CN103952453B (zh) * | 2014-05-20 | 2016-04-06 | 彭燕辉 | 一种制备海藻糖的方法 |
-
2018
- 2018-08-24 CN CN201810970927.6A patent/CN108624637B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1085096A2 (en) * | 1993-07-20 | 2001-03-21 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Preparation of high trehalose content product |
CN103946386A (zh) * | 2011-09-21 | 2014-07-23 | 株式会社林原 | 含有α,α-海藻糖二水合物晶体的粉末的制备方法 |
CN102747120A (zh) * | 2012-07-18 | 2012-10-24 | 庄晟 | 一种月饼专用转化糖浆的制备方法 |
KR20170024993A (ko) * | 2015-08-27 | 2017-03-08 | (주)무성 | 밥류 및 떡류의 전분 노화억제용 조성물 및 그 제조방법 |
CN105925642A (zh) * | 2016-06-14 | 2016-09-07 | 湖南汇升生物科技有限公司 | 用微生物发酵法工业化生产海藻糖的方法 |
Non-Patent Citations (2)
Title |
---|
Simultaneous production of trehalose, bioethanol, and high-protein product from rice by an enzymatic process;Shu-Wei Chang等;《J Agric Food Chem》;20100310;第58卷(第5期);第2908-14页 * |
海藻糖生产工艺技术研究;刘峰;《中国优秀硕士学位论文全文数据库工程科技I辑》;20140915(第09期);B016-91页 * |
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Effective date of registration: 20220215 Address after: 421800 room 211-212, zijingfu (building a-4a, Mingang new town), beside National Highway 107, TIYU North Road, Xili neighborhood committee, Wulipai street, Leiyang City, Hengyang City, Hunan Province Patentee after: Hunan Jindai Technology Development Co.,Ltd. Address before: 421800 Building 1, Dongjiang Industrial Park, Leiyang Economic Development Zone, Hunan Province Patentee before: HUNAN HUISHENG BIOTECHNOLOGY Co.,Ltd. |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method of using rice to produce trehalose Effective date of registration: 20231215 Granted publication date: 20211019 Pledgee: Agricultural Bank of China Limited by Share Ltd. Leiyang branch Pledgor: Hunan Jindai Technology Development Co.,Ltd. Registration number: Y2023980071878 |