CN108588014A - 一种猪精子培养基及其制备方法 - Google Patents

一种猪精子培养基及其制备方法 Download PDF

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CN108588014A
CN108588014A CN201810213345.3A CN201810213345A CN108588014A CN 108588014 A CN108588014 A CN 108588014A CN 201810213345 A CN201810213345 A CN 201810213345A CN 108588014 A CN108588014 A CN 108588014A
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boar spermatozoa
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melatonin
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王前
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Guangzhou Biaid Biological New Technology Development Co Ltd
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Abstract

本发明公开了一种猪精子培养基及其制备方法，包括如下成份:无水葡萄糖:18‑20g、柠檬酸三钠6‑7g、tris6‑7g、EDTA 7‑8g、碳酸氢钠1‑2g，CMC 0.1‑1g、抗结剂0.1‑1g、氯化钾0.1‑1g、柠檬酸3‑4g、半胱氨酸0.1‑1g、GHS 0.01‑0.1g、糜蛋白酶0.01‑0.05g、褪黑激素0.01‑0.05g、溶菌酶0.01‑0.1g、聚维酮碘0.01‑0.05g，本发明一种猪精子培养基及其制备方法操作制作简单先准备一份给三次蒸馏的蒸馏水1000ml，将上述培养基46g放入其中，用磁力搅拌器让其充分溶解，静置1‑2小时使可制成，且各材料成本低。

Description

一种猪精子培养基及其制备方法
技术领域
本发明涉及培养基域，尤其涉及一种猪精子培养基及其制备方法。
背景技术
现有的猪精子培养基及其制备方法操作复杂，成本高。
发明内容
本发明实施例所要解决的技术问题在于，提供一种操作方便的猪精子培养基及其制备方法。
所述猪精子培养基，包括如下成份:无水葡萄糖:18-20g、柠檬酸三钠6-7g、tris6-7g、EDTA 7-8g、碳酸氢钠 1-2g，CMC 0.1-1g、抗结剂 0.1-1g、氯化钾 0.1-1g、柠檬酸 3-4g、半胱氨酸 0.1-1g、GHS 0.01-0.1g、糜蛋白酶 0.01-0.05g、褪黑激素0.01-0.05g、溶菌酶 0.01-0.1g、聚维酮碘0.01-0.05g。
包括如下成份:无水葡萄糖:18.5g、柠檬酸三钠6.9g、tris6.5g、EDTA 7.5g、碳酸氢钠 1.3g，CMC 0.4g、抗结剂 0.6g、氯化钾 0.5g、柠檬酸 3.5g、半胱氨酸 0.15g、GHS0.03g、糜蛋白酶 0.02g、褪黑激素0.03g、溶菌酶 0.05g、聚维酮碘0.02g。
一种猪精子培养基的制备方法，包括如下步骤：先准备一份给三次蒸馏的蒸馏水1000ml，将上述培养基46g放入其中，用磁力搅拌器让其充分溶解，静置1-2小时。
实施本发明实施例，具有如下有益效果：
本发明实施例一种猪精子培养基及其制备方法，操作制作简单先准备一份给三次蒸馏的蒸馏水1000ml，将上述培养基46g放入其中，用磁力搅拌器让其充分溶解，静置1-2小时使可制成，且各材料成本低。
具体实施方式
为使本发明的目的、技术方案和优点更加清楚，下面对本发明作进一步地详细描述。
本发明实施例猪精子培养基包括如下成份:无水葡萄糖:18-20g、柠檬酸三钠6-7g、tris6-7g、EDTA 7-8g、碳酸氢钠 1-2g，CMC 0.1-1g、抗结剂 0.1-1g、氯化钾 0.1-1g、柠檬酸 3-4g、半胱氨酸 0.1-1g、GHS 0.01-0.1g、糜蛋白酶 0.01-0.05g、褪黑激素0.01-0.05g、溶菌酶 0.01-0.1g、聚维酮碘0.01-0.05g。
包括如下成份:无水葡萄糖:18.5g、柠檬酸三钠6.9g、tris6.5g、EDTA 7.5g、碳酸氢钠 1.3g，CMC 0.4g、抗结剂 0.6g、氯化钾 0.5g、柠檬酸 3.5g、半胱氨酸 0.15g、GHS0.03g、糜蛋白酶 0.02g、褪黑激素0.03g、溶菌酶 0.05g、聚维酮碘0.02g。
一种猪精子培养基的制备方法，包括如下步骤：先准备一份给三次蒸馏的蒸馏水1000ml，将上述培养基46g放入其中，用磁力搅拌器让其充分溶解，静置1-2小时。
本发明实施例一种猪精子培养基及其制备方法，操作制作简单先准备一份给三次蒸馏的蒸馏水1000ml，将上述培养基46g放入其中，用磁力搅拌器让其充分溶解，静置1-2小时使可制成，且各材料成本低。
以上所揭露的仅为本发明一种较佳实施例而已，当然不能以此来限定本发明之权利范围，因此依本发明权利要求所作的等同变化，仍属本发明所涵盖的范围。

Claims (3)

1.一种猪精子培养基，其特征在于：包括如下成份:无水葡萄糖:18-20g、柠檬酸三钠6-7g、tris6-7g、EDTA 7-8g、碳酸氢钠 1-2g，CMC 0.1-1g、抗结剂 0.1-1g、氯化钾 0.1-1g、柠檬酸 3-4g、半胱氨酸 0.1-1g、GHS 0.01-0.1g、糜蛋白酶 0.01-0.05g、褪黑激素0.01-0.05g、溶菌酶 0.01-0.1g、聚维酮碘0.01-0.05g。
2.如权利要求1所述的一种猪精子培养基，其特征在于，包括如下成份:无水葡萄糖:18.5g、柠檬酸三钠6.9g、tris6.5g、EDTA 7.5g、碳酸氢钠 1.3g，CMC 0.4g、抗结剂 0.6g、氯化钾 0.5g、柠檬酸 3.5g、半胱氨酸 0.15g、GHS 0.03g、糜蛋白酶 0.02g、褪黑激素0.03g、溶菌酶 0.05g、聚维酮碘0.02g。
3.一种如权利要求1到2任一项所述的猪精子培养基的制备方法，其特征在于，包括如下步骤：先准备一份给三次蒸馏的蒸馏水1000ml，将上述培养基46g放入其中，用磁力搅拌器让其充分溶解，静置1-2小时。
CN201810213345.3A 2018-03-15 2018-03-15 一种猪精子培养基及其制备方法 Pending CN108588014A (zh)

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CN1307480A (zh) * 1998-04-27 2001-08-08 尼卡健康产品有限公司 提高生育力的组合物及其应用
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Application publication date: 20180928