CN108587638A - A kind of biological carbon combines the preparation method of tool sheath micro- sheath algae desert crust forming agent - Google Patents

A kind of biological carbon combines the preparation method of tool sheath micro- sheath algae desert crust forming agent Download PDF

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CN108587638A
CN108587638A CN201810214956.XA CN201810214956A CN108587638A CN 108587638 A CN108587638 A CN 108587638A CN 201810214956 A CN201810214956 A CN 201810214956A CN 108587638 A CN108587638 A CN 108587638A
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sheath
algae
micro
biological carbon
desert
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孟宪刚
康小虎
闫侃
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    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/52Mulches
    • EFIXED CONSTRUCTIONS
    • E02HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
    • E02DFOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
    • E02D3/00Improving or preserving soil or rock, e.g. preserving permafrost soil
    • E02D3/12Consolidating by placing solidifying or pore-filling substances in the soil
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2105/00Erosion prevention
    • EFIXED CONSTRUCTIONS
    • E02HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
    • E02DFOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
    • E02D2300/00Materials
    • E02D2300/0004Synthetics

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Soil Sciences (AREA)
  • Materials Engineering (AREA)
  • Organic Chemistry (AREA)
  • Structural Engineering (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Inorganic Chemistry (AREA)
  • Mining & Mineral Resources (AREA)
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  • Civil Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses the preparation method that a kind of biological carbon combines tool sheath micro- sheath algae desert crust forming agent, the micro- sheath algae inoculation of tool sheath is placed in incubator in the medium, and daily illumination cultivation adds light culture to obtain algae;Rice husk is carbonized in top combustion type carbide furnace;After sand high steam processs and biological carbon be uniformly mixed to get sand biological carbon according to a certain percentage after be laid in a vinyl disc, then the micro- sheath algae powder of dry tool sheath is dissolved and is uniformly sprayed at sand biology carbon surface in deionized water;Plastics dish opening is placed in ventilation illumination box, and the daily optical culture that carries out adds light culture, in several days of the beginning of culture, finally obtains desert crust forming agent to vinyl disc supplement tap water daily.The beneficial effects of the invention are as follows the formation for significantly promoting desert skinning under drought condition, and sand-fixing effect is good, are ideal desert crust forming agents.

Description

A kind of biological carbon combines the preparation method of tool sheath micro- sheath algae desert crust forming agent
Technical field
The invention belongs to the environmental ecology recovery technique fields of biological carbon and the tool micro- sheath algae of sheath, are related to biological carbon and combine tool sheath The cultural method of micro- sheath algae desert crust forming agent.
Background technology
Biological carbon is biomass material (such as sawdust, stalk, animal waste) under conditions of anoxic or anaerobic, passes through heat A kind of solid-state carbon rich material matter that chemical conversion obtains.It is numerous research shows that biological carbon is ideal soil conditioner, improving soil Earth nutrient improves soil texture, improves soil water-retaining nutrition-retaining ability, improves edaphon abundance, diversity and improves group Fall structure etc. significant effect.
Have the micro- sheath algae of sheath (Microcoleus vaginatus) be a kind of terrestrial filamentous cyanobacteria, extreme drought, high temperature, Extremely strong adaptability is shown in the unfavorable habitats such as high salinity, high ultraviolet radioactive, is the most abundant advantage of Desert Area algae skinning Pioneer in species and deserta During Succession plants a colony biology.The tool micro- sheath algae biological breadcrust of sheath restores in deserta Have in initial period and reconstruction process and accelerates soil forming process, enhancing erosion-resisting characteristics, increase soil fertility, promote seed Library is established and the remarkable effects such as colonization of vascular plant, is arid biogeographic zone desertification treatment, the good pioneer of soil remediation, It is also the precondition of arid-desert areas vegetation succession.But tool the micro- sheath algae of sheath desert artificial infection formed biological breadcrust and The environmental factors such as inoculum density, matrix nutrition, husky water content, temperature are closely related;One piece of complete skinning clear-cutting forestland time from Several years to the more than ten years etc., the period was very long;And desert biological breadcrust can accelerate desertification process once being destroyed.Therefore Being seeded in desert surface formation biological breadcrust using the tool micro- sheath algae of sheath also needs to seek new approaches and methods.
Currently, the sustainable approach that the mankind are still effective, simple and practicable without administering the green of arid biogeographic zone Desertification. Traditional measure such as afforestation, straw checkerboard barrier sand-protecting barrier and clay sand-protecting barrier of checking winds and fixing drifting sand only has temporal effect in subrange, and And time-consuming and laborious, inefficiency, non-permanent, the basic countermeasure for solving the problems, such as soil desertification.Biological carbon combines the tool micro- sheath algae of sheath can Show to form new bio crust forming agent in desert, biological carbon therein provides carbon battalion for the growth and breeding for having the micro- sheath algae of sheath Foster, moisture and good proliferation space keep its sand-fixing effect more preferable, more permanent and simple and practicable.Our research group's joints are beautiful North Carolina State University of state has studied influence of biological carbon (rice hull carbon) addition to the micro- sheath algae growth of tool sheath, Yi Jigu for the first time Husky effect.The result shows that when ratio of the biological carbon in sand is 2%, has the micro- sheath algae biomass (being indicated with Chlorophyll-a Content) of sheath Maximum, sand-fixing effect is best, and the addition of biological carbon has been obviously promoted the formation of husky surface biological skinning under drought condition, is good Soil Biological Crusts method.
Invention content
The purpose of the present invention is to provide the preparation method that a kind of biological carbon combines tool sheath micro- sheath algae desert crust forming agent, this hairs Bright advantageous effect is to significantly promote the formation of desert skinning under drought condition, and sand-fixing effect is good, is ideal desert knot Skin agent.
The technical solution adopted in the present invention is to follow the steps below:
(1) algae culture:The tool micro- sheath algae of sheath is seeded in BG11 culture mediums, is placed in incubator, daily illumination cultivation adds Light culture, behind several days filtering obtain algae, algae drying is placed in grind in mortar and preserve;
(2) prepared by biological carbon:Rice husk is carbonized in top combustion type carbide furnace, is saved backup after being ground up, sieved;
(3) biological carbon is added in sand:It is uniformly mixed according to a certain percentage with biological carbon after sand high steam processs To sand-biological carbon;
(4) the inoculation tool micro- sheath algae of sheath:Sand-biological carbon is laid in after mixing in a vinyl disc, is then had dry The micro- sheath algae powder dissolving of sheath is uniformly sprayed at sand-biology carbon surface in deionized water;
(5) biological carbon combines the formation of tool sheath micro- sheath algae desert skinning:Plastics dish opening is placed in ventilation illumination box, The daily optical culture that carries out adds light culture, in several days of the beginning of culture, supplements tap water to vinyl disc daily, while incubator is protected Hold ventilation to keep doing, thermal environment, finally obtain desert crust forming agent.
Further, in step (1), it is 40 μm of ol photons m that daily illumination cultivation, which adds light culture method to be intensity of illumination,-2·s-1, optical culture 16h switchs to light culture 8h.
Further, husky in step (3):Biological carbon weight percent is 0%, 2%, 5%, 8%, 10%.
Further, 1g the tool micro- sheath algae powder of sheath is dried in step (4) to be dissolved in 300ml deionized waters.
Further, cultivation temperature is 30 DEG C ± 1 DEG C in ventilation illumination box in step (5).
Further, optical culture is carried out in step (5) daily light culture method is added to be intensity of illumination being 40 μm of ol photons m-2 S-1, optical culture 16h switch to light culture 8h.
Specific implementation mode
The present invention is described in detail With reference to embodiment.
(1) algae culture:The tool micro- sheath algae of sheath is derived from Jane Austen algal culture center, is seeded in BG-11 culture mediums, is placed in In pneumatic lifting photobiological incubator, 25 DEG C ± 2 DEG C of cultivation temperature, intensity of illumination is 40 μm of ol photons m-2·s-1, optical culture 16h Switch to light culture 8h, filtering acquisition algae after culture 14 days.Algae, which dries to be placed in mortar for 48 hours in 30 DEG C of baking ovens, grinds Mill, 4 DEG C of preservations.
(2) prepared by biological carbon:Rice husk is in top combustion type retort (600 DEG C of carburizing temperature, average heating rate 120 DEG C/h) in be carbonized, saved backup after being ground up, sieved (apertures 2mm).
(3) biological carbon is added in sand:Sand be derived from Tengger desert and through sieving (apertures 1mm), high steam processs it It is (husky according to a certain percentage with biological carbon afterwards:Biological carbon (w:W) ratio be 0% (control), 2%, 5%, 8%, 10%) uniformly mix It closes.
(4) it is inoculated with:Sand and rice husk biological carbon be laid in after mixing in a vinyl disc (300mm × 200mm × In 60mm), thickness is not less than 5cm.Then 1g is dried the tool micro- sheath algae powder of sheath to be dissolved in 300ml deionized waters, takes 30ml Algae solution is uniformly sprayed at sand-biology carbon surface.
(5) biological carbon combines the formation of tool sheath micro- sheath algae desert crust forming agent:Plastics dish opening is placed in ventilation illumination box Interior, cultivation temperature is 30 DEG C ± 1 DEG C, and intensity of illumination is 40 μm of ol photons m-2·s-1, optical culture 16h switchs to light culture 8h, cultivates 28 days.First 5 days started in culture, daily to vinyl disc supplement tap water 30ml.It or else keeps the skin wet later, while incubator Keep ventilation to keep doing, thermal environment.
The techniqueflow that the micro- sheath algae biological breadcrust of tool sheath is formed is combined by above-mentioned biological carbon, good desert can be obtained Crust forming agent.It is 8.2 ± 0.35mg g that the tool micro- sheath algae biomass of sheath in the biological breadcrust of formation is measured after 28 days-1(biomass is with leaf Green element a is indicated), skinning thickness can reach 21 ± 5mm, be significantly higher than control (not plus biological carbon).
Detailed description of the invention biological carbon combines tool sheath micro- sheath algae desert crust forming agent to prepare and its experiment of sand-fixing effect Verification, it is more longlasting to be formed in desert surface using the tool micro- sheath algae of sheath, it provides a kind of green more resistant to the biological breadcrust of wind erosion and has The new technique scheme of effect checks winds and fixes drifting sand to desert, the manual reversion of impaired desert ecosystem has important environmental ecology Learn meaning.
The above is only the better embodiment to the present invention, not makees limit in any form to the present invention System, every any simple modification that embodiment of above is made according to the technical essence of the invention, equivalent variations and modification, Belong in the range of technical solution of the present invention.

Claims (6)

1. a kind of biological carbon combines the preparation method of tool sheath micro- sheath algae desert crust forming agent, it is characterised in that according to the following steps into Row:
(1) algae culture:Having the micro- sheath algae inoculation of sheath in the medium, is placed in incubator, daily illumination cultivation adds light culture, if Filtering obtains algae behind dry day, and algae drying is placed in grind in mortar and preserve;
(2) prepared by biological carbon:Rice husk is carbonized in top combustion type carbide furnace, is saved backup after being ground up, sieved;
(3) biological carbon is added in sand:After sand high steam processs and biological carbon is uniformly mixed to get sand according to a certain percentage Son-biological carbon;
(4) the inoculation tool micro- sheath algae of sheath:Sand-biological carbon is laid in after mixing in a vinyl disc, then that dry tool sheath is micro- The dissolving of sheath algae powder is uniformly sprayed at sand-biology carbon surface in deionized water;
(5) biological carbon combines the formation of tool sheath micro- sheath algae desert skinning:Plastics dish opening is placed in ventilation illumination box, daily It carries out optical culture and adds light culture, in several days of the beginning of culture, supplement tap water to vinyl disc daily, while incubator keeps logical Wind to keep doing, thermal environment, finally obtain desert crust forming agent.
2. combining the preparation method of tool sheath micro- sheath algae desert crust forming agent according to a kind of biological carbon described in claim 1, feature exists In:In the step (1), it is 40 μm of ol photons m that daily illumination cultivation, which adds light culture method to be intensity of illumination,-2·s-1, optical culture 16h switchs to light culture 8h.
3. combining the preparation method of tool sheath micro- sheath algae desert crust forming agent according to a kind of biological carbon described in claim 1, feature exists In:It is husky in the step (3):Biological carbon weight percent is 0%, 2%, 5%, 8%, 10%.
4. combining the preparation method of tool sheath micro- sheath algae desert crust forming agent according to a kind of biological carbon described in claim 1, feature exists In:1g the tool micro- sheath algae powder of sheath is dried in the step (4) to be dissolved in 300ml deionized waters.
5. combining the preparation method of tool sheath micro- sheath algae desert crust forming agent according to a kind of biological carbon described in claim 1, feature exists In:Cultivation temperature is 30 DEG C ± 1 DEG C in ventilation illumination box in the step (5).
6. combining the preparation method of tool sheath micro- sheath algae desert crust forming agent according to a kind of biological carbon described in claim 1, feature exists In:Optical culture is carried out in the step (5) daily light culture method is added to be intensity of illumination being 40 μm of ol photons m-2·s-1, light training Foster 16h switchs to light culture 8h.
CN201810214956.XA 2018-03-15 2018-03-15 A kind of biological carbon combines the preparation method of tool sheath micro- sheath algae desert crust forming agent Pending CN108587638A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110294656A (en) * 2019-07-31 2019-10-01 郑州轻工业学院 One kind check winds and fix drifting sand nutrition whitewashing and preparation method thereof

Citations (3)

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Publication number Priority date Publication date Assignee Title
CN102268376A (en) * 2011-06-20 2011-12-07 中国科学院新疆生态与地理研究所 Management mode for indoor biological crust cultivation of microcoleus vaginatus and utilization thereof
CN104446691A (en) * 2013-09-24 2015-03-25 中国科学院上海高等研究院 Micro algae-biochar composite bio-fertilizer and preparation method thereof
CN106008106A (en) * 2016-05-27 2016-10-12 兰州交通大学 Sludge-based charcoal loessal soil conditioner and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102268376A (en) * 2011-06-20 2011-12-07 中国科学院新疆生态与地理研究所 Management mode for indoor biological crust cultivation of microcoleus vaginatus and utilization thereof
CN104446691A (en) * 2013-09-24 2015-03-25 中国科学院上海高等研究院 Micro algae-biochar composite bio-fertilizer and preparation method thereof
CN106008106A (en) * 2016-05-27 2016-10-12 兰州交通大学 Sludge-based charcoal loessal soil conditioner and preparation method thereof

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110294656A (en) * 2019-07-31 2019-10-01 郑州轻工业学院 One kind check winds and fix drifting sand nutrition whitewashing and preparation method thereof

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