CN108585219A - Utilize the method for composite enzyme treated sewage - Google Patents
Utilize the method for composite enzyme treated sewage Download PDFInfo
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- CN108585219A CN108585219A CN201810397238.0A CN201810397238A CN108585219A CN 108585219 A CN108585219 A CN 108585219A CN 201810397238 A CN201810397238 A CN 201810397238A CN 108585219 A CN108585219 A CN 108585219A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/001—Runoff or storm water
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The invention discloses a kind of methods using composite enzyme treated sewage, it is included in the first fermentation raw material containing malt sugar and carries out the first fermentation using plant enzyme, obtain the first zymotic fluid, then the second fermentation is carried out using first zymotic fluid in the second fermentation raw material containing malt sugar and wheat bran, obtains composite enzyme；Sewage is set to be contacted with composite enzyme, to carry out the processing of sewage.The method wastewater treatment efficiency of the present invention is good.
Description
Technical field
The present invention relates to a kind of sewage water treatment method more particularly to a kind of methods using composite enzyme treated sewage.
Background technology
With the quickening of urbanization process, the generation of municipal sewage is consequently increased.On the other hand, country wants environmental protection
The standard asked is increasingly strict.This puts forward higher requirements the technology of sewage disposal.
Traditional activated sludge process includes the following steps：Sewage disinfection treatment, sludge seeding, (appropriateness exposes sludge for agricultural use
Gas), one third of first gradually intaking simultaneously starts to be aerated, and progressively reaches design water level, starts secondary settling tank, and secondary settling tank water inlet rises
Dynamic sedimentation basin, sludge flow back at regular time and quantity.According to state regurgitant volume and time are adjusted, extra-nutrition object cultivates micro organism quantity
Sewage ability is decomposed with improving, follow-up sewage-treatment plant is started, at sludge settling pond plus chemicals, sanitizer and sludge
Manage device.Activated sludge front end is biological treatment, and the mode for taking aerobic-anaerobic to ferment decomposes harmful substance in water, in order to reach
To settled sludge and sterilisation purpose, centre uses chemicals, such as flocculant, rear end to take the modes such as carbon granule filtering that water is made to reach
Mark discharge.
However, there are problems for traditional activated sludge process.For example, processing rear end uses chemical agent, disinfection removal
Also beneficial microbe in sludge is killed while harmful substance, is made the reduction of returned sludge microbial activity, cannot fully be fermented
Decomposing harmful substances.The water activity reduction of discharge be easy to cause secondary pollution, no nutrition, it is impossible to be used in agricultural.Due to fermentation point
It is big to solve insufficient foul odour, and generates largely harmful sludge.
Invention content
In view of this, the purpose of the present invention is to provide a kind of method using composite enzyme treated sewage, without making
It is generated with the chemicals such as flocculant, processing procedure no foul smell.Specifically, the present invention includes the following contents.
The method using composite enzyme treated sewage of the present invention includes the following steps：
Composite enzyme preparation process：The first fermentation is carried out using plant enzyme in the first fermentation raw material containing malt sugar,
The first zymotic fluid is obtained, then carries out second using first zymotic fluid in the second fermentation raw material containing malt sugar and wheat bran
Fermentation, obtains composite enzyme, wherein total bacteria count is 3 × 10 in the composite enzyme5~3 × 109CFU/ml, lactic acid bacteria account for total bacterium
Several 80% or more, actinomyces, photosynthetic bacteria, nitrogen-fixing bacteria, Bacillus subtilis and saccharomycete account for 10~20%；It is walked with sewage disposal
Suddenly：Sewage is set to be contacted with the composite enzyme, to carry out the processing of sewage.
Method in accordance with the invention it is preferred that the first fermentation raw material includes 3~8 parts by weight of leaf, 3~8 weight of fruit
Part, 0.5~2 parts by weight of pine nut, 0.5~2 parts by weight of soybean residue, 0.5~2 parts by weight of soya-bean cake, 0.5~2 parts by weight of vegetable oil residue,
0.5~2 parts by weight of 0.5~2 parts by weight of rice bran and malt sugar；Second fermentation raw material includes 2~9 parts by weight of malt sugar, 1~5 weight of rice bran
Measure part, 0.5~5 parts by weight of 1~5 parts by weight of soya-bean cake, 1~5 parts by weight of soybean residue and wheat bran.In certain embodiments, first
Fermentation raw material includes 6~7.5 parts by weight of leaf, 6~7.5 parts by weight of fruit, 1~1.5 parts by weight of pine nut, 1~1.5 weight of soybean residue
Measure part, 1~1.5 parts by weight of soya-bean cake, 1~1.5 parts by weight of vegetable oil residue, 1~1.5 parts by weight of rice bran, 1~1.5 parts by weight of malt sugar.
It is further preferred that the first fermentation raw material includes leaf and each 6 parts by weight of fruit, other raw material (pine nut, soybean residue, soya-bean cake, plants
The object dregs of fat, rice bran, malt sugar) it is respectively 1 parts by weight.In certain embodiments, the second fermentation raw material includes 5~8 weight of malt sugar
Part, 2~3 parts by weight of rice bran, 2~3 parts by weight of soya-bean cake, 2~3 parts by weight of soybean residue, 0.8~2 parts by weight of wheat bran.Further preferably
The parts by weight on ground, malt sugar are 5, and the parts by weight of rice bran are 2, other each ingredients (soya-bean cake, soybean residue, wheat bran) are respectively 1 parts by weight.
Method in accordance with the invention it is preferred that plant enzyme may be used it is those of known in the art, for example, by using fruit
Ferment, pectase etc..Commercially available plant enzyme may be used, or prepared using existing method.For example,
CN103609985A, CN104012896A, CN104473170A disclose the preparation method of some fruit ferment, herein that it is complete
Text is incorporated herein.The leaf of the present invention may include Poplar leaves, pine tree leaf, Folium Pterocaryae, peach leaves, apple leaf and loguat leaf.It is preferred that
Ground, leaf are made of Poplar leaves, pine tree leaf, Folium Pterocaryae, peach leaves, apple leaf and loguat leaf.Above-mentioned leaf is provided with this field
Known meaning, which is not described herein again.The fruit of the present invention includes pears, apple, hawthorn, blueberry, orange, apricot and lichee.It is preferred that
Ground, fruit are made of pears, apple, hawthorn, blueberry, orange, apricot and lichee.In the present invention, Poplar leaves, pine tree leaf, Folium Pterocaryae,
The weight ratio of peach leaves, apple leaf and loguat leaf is 1~1.5:1~1.5:1~1.5:1~1.5:1~1.5:1~1.5；It is preferred that
It is 1~1.3:1~1.3:1~1.3:1~1.3:1~1.3:1~1.3.In the present invention, pears, apple, hawthorn, blueberry, tangerine
The weight ratio of son, apricot and lichee is 1~1.5:1~1.5:1~1.5:1~1.5:1~1.5:1~1.5:1~1.5；Preferably 1
~1.3:1~1.3:1~1.3:1~1.3:1~1.3:1~1.3.
Method in accordance with the invention it is preferred that when first ferments, the additive amount of plant enzyme is 2~6 parts by weight, excellent
Select 3~5 parts by weight, such as 4 parts by weight.It when first ferments, is aerated 20~150 minutes, fermentation time is 30~50 days.
When two fermentations, the additive amount of the first zymotic fluid is 5~15 parts by weight, preferably 6~10 parts by weight, more preferable 7~8 parts by weight.The
The fermentation times of two fermentations can be 20~50 days, such as 30~40 days.
Method in accordance with the invention it is preferred that 10~15 days fermentation temperatures are 30~40 DEG C before the first fermentation, with
Fermentation temperature afterwards is 20~30 DEG C, needs within first 20~25 days stirring 10~50 minutes daily.An implementation according to the present invention
Mode, first fermentation before 10~15 days fermentation temperature be 35~38 DEG C, subsequent fermentation temperature be 20~28 DEG C, preceding 20
Need within~25 days stirring 20~30 minutes daily.Using above-mentioned steps, is conducive to fermentation process and generates more beneficial bacteriums.
Method in accordance with the invention it is preferred that 10~15 days fermentation temperatures are 35~40 DEG C before the second fermentation, with
Fermentation temperature afterwards is 10~40 DEG C；Need within first 15~20 days stirring 10~50 minutes daily.An implementation according to the present invention
Mode, 10~15 days fermentation temperatures are 35~38 DEG C before the second fermentation, and subsequent fermentation temperature is 15~30 DEG C；Preceding 15
Need within~20 days stirring 20~30 minutes daily.Using above-mentioned steps, is conducive to fermentation process and generates more beneficial bacteriums.
Method in accordance with the invention it is preferred that in sewage treatment step, the additive amount of composite enzyme is sewage weight
0.05~5wt%, preferably 0.1~1wt%.Using the additive amount of above range, is conducive to save composite enzyme, improve simultaneously
Wastewater treatment efficiency.According to embodiment of the present invention, composite enzyme is divided into multiple batches, is separately added into multiple fermentations
Then pond is contacted with sewage successively.Wastewater treatment efficiency can further be improved in this way.
Method in accordance with the invention it is preferred that in sewage treatment step, the composite enzyme is directly and waste water.
That is, the composite enzyme can be contacted directly with sanitary sewage, to carry out fermentation decomposition to sewage.
Method in accordance with the invention it is preferred that in sewage treatment step, the composite enzyme connects with the sewage after fermentation
It touches.For example, the sewage after the composite enzyme is directly fermented with waste water first, then by the composite enzyme with
Waste water after the fermentation.
The sewage of the present invention is not particularly limited, and can be trade effluent or sanitary sewage.But from sewage disposal
For effect, sanitary sewage is preferred.According to embodiment of the present invention, the sewage is sanitary sewage.
In the method for the present invention, each microorganism in composite enzyme generates utility and secretion during growth
The matrix mutually grown and raw material are formed, stable structure, an energy are formed extensively with a variety of more by mutual Relationship With Proliferation
The biological flora of sample microbe colony, collaboration play a role, realize coexist, symbiosis, common prosperity environment purification target.
The present invention using the method for composite enzyme treated sewage from front end according to sewage yeastiness and variation of water,
Composite enzyme is launched, the beneficial microbe in sewage is activated, inhibits harmful microorganism, reaches environment purification purpose.Sludge settling
Pond front end can all aerobic fermentations, increase aeration quantity, processing procedure no foul smell generates.In addition, the present invention method without
The chemicals such as flocculant need to be added, and treated water is nutrient solution, is used for agricultural planting, can reduce or not use agriculture
Medicine, chemical fertilizer increase crop yield, realize sewage disposal recycling, and realize commercial.
Specific implementation mode
With reference to specific embodiment, the present invention is further illustrated, but protection scope of the present invention is not limited to
This.
Total bacteria count is measured using GB/T 13093-2006.Lactic acid bacteria is measured using GB4789.35-2016.Using GB/
T26428 measures other bacterial populations such as bacillus subtilis.
Fruit ferment is prepared using the method for CN103609985A disclosed embodiments 1.
Preparation example 1- composite enzymes
(1) the first fermentation required various leaves and fruit, pine nut, soybean residue, soya-bean cake, vegetable oil residue, rice bran are cleaned,
Malt sugar is diluted with 38 DEG C of warm water, spare.Raw material, which is weighed, according to the formula of table 1-1 carries out dispensing.
Table 1-1, the first fermentating formula
By 38 DEG C of water and fruit ferment, leaf (Poplar leaves, pine tree leaf, Folium Pterocaryae, peach leaves, apple leaf, loguat leaf),
Fruit (pears, apple, hawthorn, blueberry, orange, apricot and lichee), pine nut, soybean residue, soya-bean cake, vegetable oil residue, rice bran and malt sugar (add
Water dilutes, but the total amount of water is constant) mixing, it is aerated 60 minutes or so, obtains the first fermentation raw material.First fermentation raw material is being sealed
The fermentation cylinder for fermentation closed 30 days or so, obtains the first zymotic fluid.Preceding 15 days fermentation temperatures are 35 DEG C or so, subsequent 15 days
Fermentation temperature is 25 DEG C or so.It needs within first 25 days to open fermentation tank daily, stirs 30 minutes or so, then cover tightly fermentation tank.The
One successfully outstanding feature of fermenting is：Fermented liquid acetic acid taste, the distribution of zymotic fluid surface filiform.After testing, in the first zymotic fluid
Total bacteria count be 3 × 108~3 × 109CFU/ml, lactic acid bacteria are 80% or more, and actinomyces, photosynthetic bacteria, nitrogen-fixing bacteria, withered grass bud are full
Other probiotics such as bacillus, saccharomycete account for 5~20%.
(2) rice bran, soya-bean cake, soybean residue and the wheat bran needed for the second fermentation are cleaned, malt sugar is diluted with 38 DEG C of warm water, standby
With.Raw material, which is weighed, according to the formula of table 1-2 carries out dispensing.
Table 1-2, secondary fermentation formula
| Title | One time fermentation liquid | Malt sugar | Rice bran | Soya-bean cake | Soybean residue | Wheat bran | Water |
| Dosage (kg) | 10 | 8 | 2 | 2 | 2 | 1 | 75 |
38 DEG C of water is mixed with the first zymotic fluid, malt sugar, rice bran, soya-bean cake, soybean residue and wheat bran, is aerated 60 minutes left sides
The right side obtains the second fermentation raw material in closed fermentation cylinder for fermentation 30 days or so, obtains the second zymotic fluid.First 15 days fermentation temperature
Degree is 38 DEG C or so, and subsequent fermentation temperature is 25 DEG C or so；It needs within first 20 days to open fermentation tank daily, stirs 30 minutes left sides
Then the right side covers tightly fermentation tank.After testing, the total bacteria count in the second zymotic fluid is 3 × 105~3 × 109CFU/ml, lactic acid bacteria are
80wt% or more, other probiotics such as actinomyces, photosynthetic bacteria, nitrogen-fixing bacteria, Bacillus subtilis, saccharomycete account for 10~20%.
By the second zymotic fluid directly as composite enzyme；Or the second zymotic fluid is concentrated to give concentrate as compound ferment
Element；Or concentrate is dry, crushing forms pulverulent solids as composite enzyme.
Embodiment 1- sewage water treatment methods
The second zymotic fluid that above-mentioned preparation example 1 obtains is used for the sewage disposal of the present embodiment directly as composite enzyme, dirty
Water day output is 500 ton days.
1. influent quality
The water inlet of this item purpose is the sanitary sewage of resident, and water quality is shown in Table 2-1.
Table 2-1, influent quality table
| pH | Total phosphorus content TP (mg/L) | NH3-N(mg/L) | COD(mg/L) | BOD(mg/L) |
| 6-9 | 15-4 | 100-55 | 400-200 | 200-100 |
2. effluent quality controls standard
According to the country of table 2-2《Urban wastewater treatment firm pollutant emission standard》(GB18918-2002) level-one A in table
The index of standard and the field irrigation standard (GB5084-2005) of table 2-3 as water outlet qualification.
Table 2-2, water outlet contamination index numerical value list
| pH | Total phosphorus content TP (mg/L) | NH3-N(mg/L) | COD(mg/L) | BOD(mg/L) |
| 6-9 | 1 | 5 | 50 | 10 |
Table 2-3, field irrigation water quality list
3. sewage disposal process
The sewage of the present embodiment is discharged through former pond, the first fermentation vat, second fermentation vat etc. successively.Composite enzyme
Dosage and putting mode are as shown in following table 2-4, and sewage disposal result is as shown in following table 2-5.
Table 2-4, composite enzyme add situation list
Testing result compares before and after table 2-5, sewage disposal
As can be seen from the above-described embodiment, composite enzyme treated sewage using the present invention decomposes harmful substance in water；In
Between process no longer need to use chemicals (such as flocculant), rear end take carbon granule filtering etc. modes make water qualified discharge.For 500
Ton day Sewage Disposal below, without separately adding sludge treatment equipment.Water outlet be nutrient solution, be used for agricultural planting, can reduce or
Without using pesticide, chemical fertilizer, increase crop yield, realizes sewage disposal recycling, and realize commercial.Water outlet is through carbon granule mistake
Filter, meet field irrigation condition, filtered by membrane process, extract concentrated nutrition water become crops, herding nutrient solution, it can be achieved that
Commercial, discharge water meets state environmental emissioning standard.
Present invention is not limited to the embodiments described above, without departing from the essence of the present invention, this field skill
Any deformation, improvement, the replacement that art personnel are contemplated that each fall within the scope of the present invention.
Claims (10)
1. a kind of method using composite enzyme treated sewage, which is characterized in that include the following steps：
Composite enzyme preparation process：The first fermentation is carried out using plant enzyme in the first fermentation raw material containing malt sugar, is obtained
Then first zymotic fluid carries out the second hair in the second fermentation raw material containing malt sugar and wheat bran using first zymotic fluid
Ferment obtains composite enzyme, wherein total bacteria count is 3 × 10 in the composite enzyme5~3 × 109CFU/ml, lactic acid bacteria account for total bacteria count
80% or more, actinomyces, photosynthetic bacteria, nitrogen-fixing bacteria, Bacillus subtilis and saccharomycete account for 10~20%；With
Sewage treatment step：Sewage is set to be contacted with the composite enzyme, to carry out the processing of sewage.
2. according to the method described in claim 1, it is characterized in that, in composite enzyme preparation process, first proferment
Material include 3~8 parts by weight leaves, 3~8 weight fruits, 0.5~2 parts by weight pine nut, 0.5~2 parts by weight soybean residue, 0.5~
2 parts by weight soya-bean cakes, 0.5~2 parts by weight vegetable oil residue, 0.5~2 parts by weight rice bran and 0.5~2 parts by weight malt sugar；Described second
Fermentation raw material include 2~9 parts by weight malt sugars, 1~5 parts by weight rice bran, 1~5 parts by weight soya-bean cake, 1~5 parts by weight soybean residue and
0.5~5 parts by weight wheat bran.
3. according to the method described in claim 2, it is characterized in that, when first ferments, the additive amount of plant enzyme is 2~6
Parts by weight are fermented 30~50 days；When second ferments, the additive amount of the first zymotic fluid is 5~15 parts by weight, fermentation 20~50
It.
4. according to the method described in claim 3, it is characterized in that, 10~15 days fermentation temperatures are 30 before the first fermentation
~40 DEG C, subsequent fermentation temperature is 10~40 DEG C, needs within first 20~25 days stirring 10~50 minutes daily.
5. according to the method described in claim 3, it is characterized in that, 10~15 days fermentation temperatures are 35 before the second fermentation
~40 DEG C, subsequent fermentation temperature is 20~30 DEG C；Need within first 15~20 days stirring 20~50 minutes daily.
6. according to the method described in claim 1, it is characterized in that, in sewage treatment step, the addition of the composite enzyme
Amount is 0.05~5wt% of sewage weight.
7. according to the method described in claim 1, it is characterized in that, in sewage treatment step, the addition of the composite enzyme
Amount is 0.1~1wt% of sewage weight.
8. according to the method described in claim 6, it is characterized in that, in sewage treatment step, the composite enzyme directly with
Waste water.
9. according to the method described in claim 6, it is characterized in that, in sewage treatment step, the composite enzyme and fermentation
Waste water afterwards.
10. according to claim 1~9 any one of them method, which is characterized in that the sewage is sanitary sewage.
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Cited By (1)
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| CN109250883A (en) * | 2018-11-27 | 2019-01-22 | 内蒙古济世源环保生物科技有限公司 | Method for sludge treatment |
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| CN109250883A (en) * | 2018-11-27 | 2019-01-22 | 内蒙古济世源环保生物科技有限公司 | Method for sludge treatment |
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