CN108576382A - A kind of feeding composite yeast bacteria additive - Google Patents

A kind of feeding composite yeast bacteria additive Download PDF

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CN108576382A
CN108576382A CN201810511766.4A CN201810511766A CN108576382A CN 108576382 A CN108576382 A CN 108576382A CN 201810511766 A CN201810511766 A CN 201810511766A CN 108576382 A CN108576382 A CN 108576382A
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freeze
dried powder
powder
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sea
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张奎昌
杨宪勇
花丽
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Xuzhou Blue Canal Agricultural Resources Development Co Ltd
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Xuzhou Blue Canal Agricultural Resources Development Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A kind of feeding composite yeast bacteria additive of the present invention, it is characterized in that being uniformly mixed by saccharomycete, head mold, trichoderma, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, the fermentation egg-shell meal for adding surplus again is uniformly mixed, and composite packaging is made:20000 100,000,000 hundred million, saccharomycete in every 1000g additives, head mold 8000 100,000,000 hundred million, trichoderma 8,000 30000 hundred million, 5 50g of sea-buckthorn freeze-dried powder, 5 50g of Rosa roxburghii freeze-dried powder, 3 30g of wintercherry freeze-dried powder, 3 30g of blackcurrant freeze-dried powder, 50 100g of cactus freeze-dried powder, remaining is fermentation egg-shell meal.The present invention is clear containing viable count, has specific application and Quantitatively Selecting conducive to user.The present invention is using sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder and fermentation egg-shell meal as the matrix of additive, not only improve the nutritive value of composite yeast bacteria additive, promote the metabolism of animal simultaneously, improve the disease resistance of body, promote growth of animal, finally improves the economic benefit of its aquaculture.

Description

A kind of feeding composite yeast bacteria additive
Technical field
The invention belongs to feed additive fields, are related to a kind of raising containing viable bacterias such as saccharomycete living for cultivated animals Feed additives.More particularly to a kind of feeding composite yeast bacteria additive.
Background technology
The saccharomycete being currently known has more than 490 kinds.The relationship of saccharomycete and the mankind are more close, therefore gradually form A kind of independent fungi group, classify widely used Luo De(Lodder)Monograph《Saccharomycete》(1970)In sorting technique. In modern industry, since saccharomycete is typical unicellular microorganism, he has, and individual is big, protein equal size is high, easy point It, easily culture, metabolite more, integrated application wide the features such as strong from, omnivorousness, other than being used for brewing drinks, alcohol, is additionally operable to Produce glycerine, food yeast, yeast for animal feeds, oil dewaxing, fermentation production of organic acid, enzyme preparation etc..Simultaneously as yeast cells In rich in ribonucleic acid, cromoci, coacetylase etc., saccharomycete be also pharmaceuticals industry, biotechnology industry important source material, yeast It is rich in protein and a variety of B family vitamins in thalline, currently there is relatively broad application in feed industry.Utilize saccharomycete With head mold(Rhizopus), trichoderma(Trichoderema)A kind of feed addictive is formed, it can not only be effectively after feeding animals Promote digestion, absorption of the livestock and poultry to feed, improves rate of body weight gain, fed especially with fermentation, using effect becomes apparent.Wherein Head mold is the products such as manufacture carbohydrase, a- amylase, steroid hormone, fumaric acid using bacterium.Trichoderma mainly generates cellulose Enzyme, it is also wine brewing, food processing, roughage fermentation etc. using bacterium.Existed using yeast, head mold, trichoderma alternate culture Containing base-material fiber degradation on the material such as the higher straw powder of crude fibre, health bran, is made, crude protein content is improved, can be used as feed adjusting The leavening of agent and roughage.But just how current many manufacturing enterprises are with regard to select microbe carrier(That is filler(Agent))System Relatively stable biological feed additive, that also does is inadequate, often only take into account raw material be easy to get and production cost, Chang Xuan With rice bran, wheat bran, corn flour, maize cob meal, peanut shell powder, oyster shell whiting, vermiculite power, mountain flour calcium carbonate, medical stone powder, zeolite powder One or more of mixture, some also use straw or grass meal as filler material.These are examined in line with production cost Consider, then has ignored the stability of microbial bacteria and the trophism value of feed addictive.
Some in microbial bacteria additive in order in use, have certain adhesion, be such that microbial bacteria raises
Feed additives can be adsorbed on animal feed when being mixed in admixing animal feed, convenient for equal in animal feeding Even property and the maximization for being fed into bacterium amount, some products take pains a certain amount of binder of addition.
Some continue in order to make the relative constant of raising animal stomach inner pH value after so that the foodstuff being fed into is entered gastrointestinal tract The effect of reaching acidification often utilize the acidulants such as citric acid, lactic acid, hydrochloric acid, fumaric acid, but these acidulants simultaneously in the product It not only directly locally causes acidity too strong microbial bacteria contained by additive, influences the survival of thalline, meanwhile, these acidifying substances To cultivated animals also without the nutritive value directly utilized, meanwhile, there is prodigious stimulation to the cub of livestock and poultry, the gastrointestinal tract of young fowl Property, compatibility is using not ideal enough.
Exploitation one kind can overcome above-mentioned deficiency, can either keep stability, the trophism of institute's mycetome, have again to thalline There is adhesion, while the filler to using has the additive for microbe feedstuff that nutritive value is provided to cultivated animals again, is Promote the basis of feed additive industry long-term stability development.
Invention content
The purpose of the present invention is to provide a kind of new feeding composite yeast bacteria additive, the feeding mulriple yeasts additions Agent not only there is thalline to stablize, and the thalline time-to-live is long, and used auxiliary agents have certain adhesiveness and absorption Property, thalline can be made preferably to be bonding on one with auxiliary agents, meanwhile, complementary additive has certain acidity, to dynamic After object feeding, abundant nutriment can not only be provided, while its acidic materials can keep stomach inner pH value relative constant, into stomach The effect of acidification is continued up to after enteron aisle.The purpose is to provide a kind of more feeding mulriple yeasts of stability and trophism feeding Feed additives.The additive can be used as feed conditioning agent and the leavening of roughage, can also be used as feed addictive use In mixed feed.
A kind of feeding composite yeast bacteria additive of the present invention, it is characterized in that by saccharomycete, head mold and trichoderma with it is a certain amount of Seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, blackcurrant powder, cactus powder and fermentation egg-shell meal uniformly mix, and obtain a kind of new feeding multiple Yeast bacteria additive is closed, the thalline of this additive is stablized, and the thalline time-to-live is long, and seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, black Gallon powder sugar content is high, and especially all there is cactus powder preferable Adhesion property, fermentation egg-shell meal also to have preferable absorption Property.Wherein sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant are the ascorbic fruits of high-content, and acidity is extremely strong, not only give cultivated animals The nutriments such as abundant vitamin are provided, the acidic materials after feeding play the effect for promoting digestion well, to The purpose of the present invention is highlighted.
In order to realize the present invention, the following technical solutions are specifically adopted:
A kind of feeding composite yeast bacteria additive, feature it be first by saccharomycete, head mold, trichoderma, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt Freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus fermentation egg-shell meal it is mixed It closes uniformly, composite packaging is made:20000 hundred million -60000 hundred million, saccharomycete, head mold 8000 hundred million-in every 1000g additives 30000 hundred million, trichoderma 8000-30000 hundred million, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, Blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-100g, remaining is fermentation egg-shell meal.
Saccharomycete described above is appointing in Candida, red yeast, brewer's yeast, brewer's yeast, fodder yeast Meaning is a kind of.
Saccharomycete described above is saccharomycete dry powder product;Head mold described above is rhizopus dry powder product;It is above-mentioned The Trichoderma is Trichoderma dry powder product.
The saccharomycete dry powder product, the rhizopus dry powder product, the Trichoderma dry powder product, can make 200 hundred million preparations, the ferment are no less than with or as every gram of feed addictive containing viable bacteria concentration with food commercially available Female bacterium dry powder product, rhizopus dry powder product, Trichoderma dry powder product and the weight proportion of the filler material in formula are done by thalline The thalline content of powder and final products determines.
The saccharomycete dry powder product, rhizopus dry powder product, Trichoderma dry powder product can also utilize existing method Select the culture medium accordingly optimized then will be in zymotic fluid with high speed centrifugation mode respectively by liquid fermentation equipment culture Thalline is separated, and is placed in dry indoor cold wind or bacterium mud drying is made with vacuum freeze drying mode, obtain respectively Every gram of viable bacteria concentration no less than 200 hundred million thalline dry powder product.
Sea-buckthorn freeze-dried powder described above, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, It being made by following methods, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively, The impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, remove seed step;Rinsing condition described above is The citric acid of weight 0.1% is added with 40 DEG C of water, rinses 15min, and be stirred continuously, and is pulled out to be put into pure water and is rinsed 3 times;On It is that will be cooled to -45 DEG C or less rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively to state freezing;Above-mentioned distillation Dry process conditions are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w;It is above-mentioned Low-temperature grinding is the crushing that temperature of charge is in 0 DEG C or less progress, 100 mesh of grinding particle size >.
Composite packaging described above uses interior outer bag composite packaging, inner bag that polypropylene plastics pocket, outer packing is used to use aluminium Foil bag is filled with silica-gel desiccant pouch between interior outer bag.
Fermentation egg-shell meal described above, is to clean up eggshell, dries moisture, in advance drying process 1-2 hours, so After be ground into 40-50 mesh powder, suitable water, which is added, makes into water content in 45-55%/w, adds the ferment of egg-shell meal weight 20% Female bacterium is mixed thoroughly, is fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, after fermentation, fermentation materials is adjusted to temperature Carbonyl ammonia is carried out under the conditions of being 80-90% for 82-92 DEG C, humidity(Also referred to as Maillard reaction)Reaction 22-28 days, then, by material Moisture < 5%/w are dried under the conditions of no more than 100 DEG C using electric baking case, through crushing, are sieved with 100 mesh sieve to obtain the final product.
Drying described above uses heated-air drying or far-infrared ray drying;Dry condition is 180 DEG C -300 DEG C, drying Purpose one be to reach to kill the harmful substances such as microorganism and parasite, second is that the crisp matterization of eggshell can be made to be conducive to crush, and make powder Egg-shell meal after broken is loose conducive to fermentation.
The carbonyl ammonia reaction, i.e. Maillard reaction, also known as " non enzymatic browning reacton ", are carbonyls(Reduced sugar Class)And amino-compound(Amino acid and protein)Between reaction.
The above-mentioned purpose that eggshell coarse powder is used to saccharomycetes to make fermentation, first, can effectively make eggshell fully soft using fermentation process Change, keep the egg-shell meal after fermentation loose, is easy to mix with unclassified stores;Second is that can effectively be made in eggshell using saccharomycetes to make fermentation The ingredients such as calcium obtain effective bioconversion, form BIOLOGICAL CALCIUM, conducive to being absorbed and utilized for animal, saccharomycetes to make fermentation can make fermentate Material forms the mass propagation of more protein, especially saccharomycete, and it is peculiar to increase yeast institute for the aggregation for the ingredient that can have additional nutrients The multiple nutritional components such as glucose, protein, nucleic acid, vitamin, carbohydrate, lipid-like substances, especially B races dimension life Element such as B1、B2、B6、B12And the physiological activators such as folic acid, niacin, pantothenic acid, inositol get a promotion, these beneficial nutrients Matter ingredient undoubtedly plays the nutritional ingredient for improving product of the present invention, improves its nutritive validity.
The further carbonyl ammonia of egg-shell meal is reacted in the present invention, first, promoting the maturing process of eggshell, is further made wherein Calcareous ingredient obtain sufficient bioconversion, biological calcium component is formed, second is that the protein component in material is made to obtain fully Bioconversion, amino acid is degraded to, third, a large amount of aminoacid ingredients to be formed is made to be converted into the small molecules object such as small peptide and small peptide Matter, conducive to digesting and absorbing after animal feeding, the reaction of carbonyl ammonia not only promotes the trophic function of product, after the reaction of carbonyl ammonia Material can further increase gas porosity and be conducive to the application of mix feed, while make carbohydrate-modifying to be reduced sugar, produce simultaneously Raw abundant fragrance matter, not only improves the anti-immunity ability of product, further improves the flavor of product, increase the eating of animal Property.
Eggshell described above is the mixed of any one or a few in egg shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell Close eggshell.
Saccharomycete described above(saccharomyces)For candida utili(Candida utilis), the torrid zone it is false Silk yeast(Candida tropicalis), brewer's yeast(Saccharomyces cerevisiae), brewer's yeast (Saccharomyces cerevisiae)And fodder yeast(Candida Tropicali)In any one.
Above-mentioned saccharomycete can be selected using all viable bacterias as food additives or feed addictive commercially available Concentration is no less than 200 every gram hundred million thalline dry powder products.
Existing method preparation can also be used in above-mentioned saccharomycete, such as can accordingly be optimized according to the selection of the property of strain Then thalline in zymotic fluid is separated with high speed centrifugation mode, is set by Liquid Culture Zymolysis Equipment culture by culture medium Bacterium mud drying is made in the indoor cold air drying of drying or with freeze-drying mode, it is every gram to obtain viable bacteria concentration respectively 200 hundred million thalline dry powder products.
A kind of prioritization scheme of feeding composite yeast bacteria additive of the present invention is, first by saccharomycete, head mold, trichoderma, sand Spine freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus The egg-shell meal that ferments is uniformly mixed, and composite packaging is made:30000 hundred million -60000 hundred million, saccharomycete, root in every 1000g additives Mould 10000 hundred million -30000 hundred million, trichoderma 10000 hundred million -30000 hundred million, sea-buckthorn freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder 8- 50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 60-100g, remaining is fermentation egg-shell meal.
The further prioritization scheme of the present invention is a kind of feeding composite yeast bacteria additive, it is characterized in that first by yeast Bacterium, head mold, trichoderma, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, the mixing of cactus freeze-dried powder are equal It is even, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging is made:40000, the saccharomycete in every 1000g additives Hundred million -60000 hundred million, head mold 12000 hundred million -30000 hundred million, trichoderma 12000 hundred million -30000 hundred million, sea-buckthorn freeze-dried powder 15- 50g, Rosa roxburghii freeze-dried powder 12-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 70-100g, Remaining is fermentation egg-shell meal.
The further prioritization scheme of the present invention is a kind of feeding composite yeast bacteria additive, it is characterized in that first by ferment Female bacterium, head mold, trichoderma, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, the mixing of cactus freeze-dried powder Uniformly, then add surplus fermentation egg-shell meal be uniformly mixed, composite packaging is made:The saccharomycete 50000 in every 1000g additives A hundred million -60000 hundred million, head mold 15000 hundred million -30000 hundred million, trichoderma 15000 hundred million -30000 hundred million, sea-buckthorn freeze-dried powder 20- 50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder 80-100g, Remaining is fermentation egg-shell meal.
Saccharomycete (saccharomyce) is a kind of unicellular microorganism, and yeast cell wall main component is glucan, carefully Intracellular contains the multiple nutritional components such as abundant protein, nucleic acid, vitamin, carbohydrate, lipid material, minerals.Its The main nutrient composition of dry yeast is:Protein 40-50%, fat 1.5%, ash content 7%, crude fibre 1.5%, carbon hydrate Object 30-35%, moisture 5-7%.In addition, saccharomycete is also rich in B family vitamin, such as vitamin B1、B2、B6、B12, and wheat steroid The physiological activators such as alcohol, niacin, folic acid, pantothenic acid, inositol, the wherein content of wheat sterol and selenium are compared with horn of plenty.Except Yeast protein Other than matter, yeast metallothionein(MT)It is also a kind of low molecular weight, rich in cysteine, the metal that can be generated by metal inducement Binding protein.It is primarily involved in storage, transport and the metabolism of trace element, has and removes hydroxyl radical free radical, antagonism ionization and non-electrical From different physiological roles such as radiation and heavy metal detoxifications.
Yeast Glutathione peptide content is up to 1%, and the content of material is up to 8% in homoglutathion yeast.Glutathione is joined Contain Fe with internal redox reaction, activation SH enzymes and raising2+The effects that enzymatic activity is a kind of important biochemical drug.Paddy The sweet peptide of Guang can shorten dough kneading time and resting period;As antioxidant, the holding time of fried food can be extended, extracted Object is more preferable than glutathione effect is used alone;As feed addictive, animal's liver function is protected, improves fish growth, is increased Yield with milk.
B family vitamin content is up to 2% in yeast, and B family vitamin is also important biochemical drug, has a variety of important Biological function, such as glycometabolism, amino acid metabolism, transmethylation etc..
Also the ergosterol containing higher level in yeast, ergosterol is the precursor substance of vitamin D, in ultraviolet lighting It penetrates down and is converted to vitamin D.
Yeast cell wall polyoses content reaches 30%-35%, and there is growth promotion, antiviral, enhancing to be immunized, adjust the micro- life of enteron aisle State environment, the effect for promoting Development of Immune Organs.Literature has shown that by yeast, head mold, the culture of trichoderma alternate containing crude fibre compared with On the base-materials such as high straw powder, bran, using different inoculation times, the lignocellulose degradation 12.9%-17.43% of base-material can be made, Crude protein improves 6.8%-11.7%, if being handled through low temperature drying, product is rich in saccharomycete, carbohydrase, pectase, cellulose Enzyme.The product can be used as feed conditioning agent and the leavening of roughage, can also be used as feed addictive in mixed feed. Show that the product can be effectively facilitated digestion, absorption of the livestock and poultry to feed through feeding comparison, improves rate of body weight gain 5%-15%, especially It is using fermentation feeding, using effect is fairly obvious(Shi Bo is edited, and novel fodder additive is develop and useedd, and chemical industry goes out Version society August the 1st edition, page 120 in 2005).
Sea-buckthorn(Hippophae rhamnoides Linn.), fruit contains abundant nutriment and biological active matter Matter can be widely applied to many fields of the national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fishery.Fructus hippophae and oil With very high medical value, there is a cough-relieving apophlegmatic, relieving dyspepsia, promoting blood circulation to remove blood stasis effect, can treat gastric duodenal ulcer with And indigestion etc..
Sea buckthorn fruit is full of nutrition, according to surveying and determination in its fruit containing multivitamin, aliphatic acid, trace element, sub- oil element, The various amino acid of Hippophate flavone, superoxides isoreactivity substance and needed by human body.Wherein Vitamin C content is high, every 100 grams In fruit juice, Vitamin C content can reach 825-1100 milligrams, be 2-3 times of Kiwi berry, be known as the laudatory title of the king of vitamin C.Contain Sugared 7.5%-10%, the 3%-5% containing acid.
Rosa roxburghii Tratt(Rosa roxbunghii)For the fruit of the perennial machaka rosa roxburghii of the rose family also known as mountain king fruit, thorn Certain kind of berries fruit, thatch pears etc..With clearing heat and promoting fluid, stomach strengthening and digestion promoting, relieving summer-heat and other effects.Rosa roxburghii Tratt contains abundant vitamin B, C, carrotene, The ingredients such as malic acid, citric acid are usually used in deficiency of stomach-Yin, anorexia, indigestion or eating accumulation, glutted full bored, diarrhea Loose stool;Pyreticosis or hot summer weather hinder Tianjin, thirsty, scanty drak urine.
Ripe Rosa roxburghii Tratt is fleshy hypertrophy, sweet and sour, fruit are rich in sugar, vitamin, carrotene, organic acid and more than 20 kinds of ammonia Base acid, more than 10 the kinds trace element and superoxide dismutase, especially Vitamin C content beneficial to human body are high, are to work as Highest in preceding fruit, 841.58 milligrams -3541.13 milligrams of content in every 100 grams of fresh fruits is 50 times of citrus, Kiwi berry 10 times, the laudatory title with " king of vitamin C ".Fruit of Grossularia burejensis Berger has very high nutritive value and medical value, sour, puckery, flat, Digestion-promoting spleen-invigorating restrains stopping leak, for treating having indigestion abdominal distension, dysentery, enteritis, vitamin C deficiency etc..
Wintercherry(Physali alkekengiL.)Also known as groundcherry, franchet groundcherry persistent calyx or fruit, cape gooseberry etc..Containing more in Fruit of Physalis Kind of nutritional ingredient, the content of wherein calcium is 73.1 times of tomato, 13.8 times of carrot, and ascorbic content is tomato 6.4 times, 5.4 times of carrot.With unique flavor and abundant nutrition.Fruit of Physalis aromatic flavour, delicious.Berry is rich Containing vitamin C, beta carotene, more than 20 kinds of mineral element and 18 kinds of amino acid, there is heat-clearing, removing toxic substances, diuresis effect to be usually used in It controls in heat-syndrome cough, pharyngalgia, jaundice, dysentery, water, the treatment of furunculosis, erysipelas etc..
Blackcurrant scientific name elements of black currant(Ribes nigrum L.)Also known as currant, purple plum etc., ripening fruits are black Small berries, it is interior rich in vitamin C, anthocyanidin.Blackcurrant is not only a kind of delicious flavour, fruit full of nutrition, and has Good medicinal efficacy.Blackcurrant fruit is rich in mineral matter element, and the Zn content in mineral matter element is high, can be used as and comes for zinc Source;Ascorbic content is high, hence it is evident that higher than citrus, hawthorn, also contains phosphorus, magnesium, potassium, calcium, anthocyanidin, aldehydes matter.Seed Linoleic acid content is high.Ascorbic content is very abundant in blackcurrant, it is often more important that also contains a large amount of antioxygen and is melted into Point, this assures ascorbic function and effect.Blackcurrant is rich in such as anthocyanidin, vitamin C, flavones, Quercetin, red bayberry The substances such as alcohol, phenolic acid, catechin and blackcurrant polysaccharide, these are all the bioactive substances with good anti-oxidation function.
Cactus(Scientific name:Opuntia stricta(Haw.)Haw.var.dillenii(Ker-Gawl.) Benson), It is a kind of plant of Opuntia.Alias celestial being palm, kwan-yin palm etc..Cactus is the meat shrub that grows thickly, the wide ovum of top branch Shape, obovate elliptic or subcircular;Flower spoke shape, holder obovate.It is used as medicine with complete stool.The four seasons can adopt.
Cactus is cool in nature, sour, nontoxic.Have the promoting flow of qi and blood circulation, heat-clearing toxin-expelling functions.Mainly have clearing heat and detoxicating, eliminating stasis to subdue swelling, The functions such as the promoting flow of qi and blood circulation, benefiting stomach and stopping pain.Cactus contains amino acid and various trace elements in needed by human 8, and embraces green The preciousness ingredient such as lotus, Kok Beattie cents, cholla stem, starches through cactus crude drug and analyzes, contain pectin, polysaccharide and glue exudate.From herb point From extracting to obtain freidelin ketone(friedelin), -3 α -ol of freidelin(friedelan-3-α-ol), taraxerone (taraxerone)And taraxerol(taraxerol).
Eggshell, what quantity was larger in China's poultry farming has chicken, duck, goose and quail etc., using egg, duck's egg, As in food processing process, the useless eggshell enormous amount generated is provided according to existing disclosed report for goose egg and quail egg Material display, the eggshell total amount that a medium-sized city is monthly discarded reach 60-100 tons, and account for egg size by Eggshell weight 11% calculates, The eggshell that about 1,500,000 tons of the daily output in China, not only pollutes environment, but also waste of resource in this way.Discarded eggshell is integrated It utilizes, resource utilization can not only be improved and avoids environmental pollution, and be greatly improved the economic benefit and social benefit of enterprise.
Wherein egg shell is in China's yield maximum, and egg shell accounts for the 10-12% in entire egg, by under film, shell on shell Film and eggshell three parts composition, eggshell is a kind of calcareous hard shell, is located on shell between film and shell lower film.In the change of egg shell In studying point, minerals account for 95%, and protein 3.3%, moisture 1%, fat 0.03%, remaining is carbohydrate and other have Machine substance(Certainly, a large amount of remaining egg white in eggshell in the above ingredient are not included).Eggshell mineral ingredient is mainly Calcium carbonate accounts for 94% or so, remaining is magnesium carbonate and calcium phosphate, and calcium, phosphorus content account for 34.9% and of eggshell dry matter respectively 0.2%.In eggshell, seven kinds of vitamins and various trace elements and some important enzyme, peptide matters can be measured, he Although content it is seldom, be all the substance needed in human body or animal body.
In the prior art, it is replenished the calcium with egg-shell meal, has the product of the grinds after sterilization by eggshell in people's product, also have The application of organic calcium is prepared into using primary or secondary clacining method.It is at present most widely in drug, food and nutriment In preparation, micron order, submicron order and nano level superfine powder are mostly used, is added to various food, nutrition kind, has reached very Good effect.Application of the eggshell in China's animal-breeding is taken mostly by eggshell cleaning, drying, and milling makes an addition in feed The method of feeding, since grinding particle size is larger, being absorbed and utilized for animal is poor, can't reach good application effect.
The present invention is first to be ground into coarse powder by after eggshell cleaning, high temperature drying, fermented using saccharomycete, then again Carbonyl ammonia reaction treatment is carried out, then dried, crush and be prepared into biological calcium product, the program not only improves what eggshell utilized Nutrition, while the main composition-calcium carbonate in eggshell being made to obtain biochemical reaction, the biological calcium carbonate for being more conducive to absorb is formed, Carbonyl ammonia reaction again so that its protein component is further converted to amino acid, make macromolecular protein, it is amino acid converting be small peptide Or the small-molecule substances such as small peptide, the advantageous effect for reaching collection eutrophy and being absorbed and utilized.
The beneficial effects of the invention are as follows:
(1)Product of the present invention can highlight the viable bacteria content of saccharomycete, head mold and trichoderma, significantly improve thalline in closed compound packet Survival rate in dress can be obviously improved the stability of product.
(2)Pass through sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder and ferment Female mycorhiza is mould, trichoderma mixing, due to the stickum in the colloidal substance that these dry powder formulations are rich in, especially cactus, energy The adhesive effect between additive and feed is preferably increased, and improves the utilization rate of additive;
(3)By sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder high-content amino acid, vitamin, mineral element, more Sugar.The nutritional ingredients such as the vitamin C of organic acid, especially high-content and various bioactivators, to improving animal immune energy Power is simultaneously obviously improved meat, Egg Quality, the acidic materials in especially above-mentioned dry powder, utilization, promotion animal to contained microorganism Digestion all has significant effect;
(4)Due to using Freeze Drying Technique means, enable sea-buckthorn obtained, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder Its active constituent is maximumlly remained, has distinctive sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the smell of cactus application and taste Taste, free from extraneous odour add the preference of animal.
(5)Using egg-shell meal first through saccharomycetes to make fermentation, carbonyl ammonia is then used(Maillard reaction)Reaction is prepared into rich in life The fermentation egg-shell meal of object calcium, not only makes the calcium carbonate in eggshell obtain bioconversion, obtains the BIOLOGICAL CALCIUM for being conducive to animal absorption, And further converted to forming a large amount of protein, amino acid, carbohydrate after saccharomycetes to make fermentation, make amino Acid, small peptide or small peptide small-molecule substance get a promotion, and more conducively animal is absorbed and utilized, and passes through and converts what carbohydrate was formed Inverted sugar and fragrance matter further improve the flavor and taste of product, increase preference of the animal to product.
(6)To the preparation of sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder in the present invention, do not remove the peel, remove seed step Suddenly, it is therefore an objective to by being crushed after freeze-drying, so that its skin and seed is worn into fine powder, make anthocyanidin, oil in its skin and seed Class, organic acid, bud fragrance substance are retained, and the nutritive value of its freeze-dried powder is promoted.
(7)The present invention is lyophilized using sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus Powder, matrix of the fermentation egg-shell meal as additive contain constants such as a large amount of calcium, phosphorus, potassium, sodium, magnesium, zinc and micro in matrix Element still contains abundant vitamin C, anthocyanidin, beta carotene, malic acid, citric acid, protein, amino acid, nucleic acid, Portugal Grape sugar, lipoidis, carbohydrate, B family vitamin such as B1、B2、B6、B12And the physiological activity such as folic acid, niacin, pantothenic acid, inositol Substance, not only improves the nutritive value of composite yeast bacteria additive, while promoting the metabolism of animal, improves the disease-resistant of body Ability promotes growth of animal, finally improves the economic benefit of its aquaculture.
(8)The feeding composite yeast bacteria additive of the present invention acts not only as feed addictive in mixed feed, going back It can be the leavening of feed conditioning agent and roughage.
(9)Product of the present invention uses interior outer bag composite packaging, interior outer bag interlayer to be filled with silica-gel desiccant, and product does not return Tide, no caking, product is loose, good fluidity, can preferably keep exterior quality and inherent quality.
Operation to its technical process for not having to state in detail in the present invention, be those skilled in the art common sense in Operation.With regard to the purchase and storage of heretofore described sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant fresh fruit and fresh cactus, the present invention The construction of thousand Co., Ltds of Yao Tang traditional Chinese medical science research institute of cooperation unit Jiangsu has 20,000 tons of freezer facility, is to have to storage fresh fruit material It ensures enough.
Specific implementation mode
Embodiment 1
The preparation of sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder:
Sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the cactus for taking fresh mature respectively, remove carpopodium, and the impurity such as rotten fruit are heavy with containing 40 DEG C of aqueous solutions of 0.1% citric acid are measured, 15min is rinsed under constant stirring and pulls out, be put into pure water and rinse 3 times, pull out It is drained;It will be moved into refrigerator after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively, be cooled to -45 DEG C rapidly Below;Respectively by the sea-buckthorn freezed, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus move into lyophilization machine in, sublimation temperature control- 22--32 DEG C, vacuum degree 120-150Pa makes dry materials to moisture < 6%(w), the time about needs 18 hours, is put into after taking-up In freezing crusher, control material temperature is no more than 0 DEG C, after being crushed to 100 mesh of fineness >, respectively obtains sea-buckthorn freeze-dried powder, thorn Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are fitted into polybag and seal, and are stored in 0 DEG C or less It is spare in freezer.
Embodiment 2
Using the culture medium accordingly optimized, candida utili, torrid zone vacation are individually cultivated using Liquid Culture Zymolysis Equipment Silk yeast, brewer's yeast, brewer's yeast and fodder yeast, are then separated the thalline in zymotic fluid with supercentrifuge, Bacterium mud is dried with vacuum freeze drying mode, respectively obtain viable bacteria content be 200 hundred million/grams candida utili dry powder, Candida tropicalis dry powder that viable bacteria content is 200 hundred million/grams, the brewer's yeast dry powder that viable bacteria content is 200 hundred million/grams, work The feed yeast powder that the brewer's yeast dry powder and viable bacteria content that bacterial content is 200 hundred million/grams are 200 hundred million/grams.
Embodiment 3
The preparation of fermentation egg-shell meal:
One or more of mixing eggshells of egg shell or Ovum Anas domestica putamina, goose egg-shell, quail eggshell are cleaned up, moisture is dried, sets Enter in baking oven, after 1.5 hours, taking-up is let cool heated-air drying under the conditions of 240 DEG C, is ground into 45 mesh powder;It is public to weigh egg-shell meal 80 Jin, suitable pure water, which is added, makes into material moisture 50%(W), add the feed yeast powder 20 that embodiment 2 obtains Kilogram, it fully mixes rear sabot thoroughly, is placed in fermenting cellar, ferment 60-72 hours under the conditions of temperature is 31-33 DEG C, wait for fermentation ends Afterwards, it is 82 DEG C -92 DEG C, under the conditions of temperature is 80-90% fermentation materials to be adjusted to temperature, carries out carbonyl ammonia and reacts 22-28 days, so Material is dried to moisture < 5% using electric baking case under the conditions of no more than 100 DEG C afterwards(W), utilize omnipotent crushing Machine crushes, and crosses 100 sieves to get fermentation egg-shell meal.
With the preparation method of the fermentation egg-shell meal of embodiment 3, it is dry that the candida utili obtained in embodiment 2 can be respectively adopted Powder, candida tropicalis dry powder, brewer's yeast dry powder or brewer's yeast dry powder carry out preparing fermentation egg-shell meal, other process conditions Implement according to eggshell powder preparation technique parameter of fermenting in the application claim 1-4.
Embodiment 4
A kind of feeding composite yeast bacteria additive, be by saccharomycete dry powder, rhizopus dry powder, Trichoderma dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation eggshell of surplus Powder is uniformly mixed, and composite packaging is made:The saccharomyces cerevisiae 20000 hundred million in every 1000g additives, rhizopus 30000 hundred million, Trichoderma 30000 hundred million, sea-buckthorn freeze-dried powder 5g, Rosa roxburghii freeze-dried powder 50g, wintercherry freeze-dried powder 3g, blackcurrant freeze-dried powder 3g, cactus Freeze-dried powder 100g, remaining is fermentation egg-shell meal;Specifically production method is:Weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, thorn respectively Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder mixing, then by saccharomyces cerevisiae, rhizopus, trichoderma Bacterium dry powder is uniformly admixed, and after then addition surplus is sufficiently mixed uniformly by the fermentation egg-shell meal obtained of embodiment 3, carries out compound packet Dress;Composite packaging is that inner bag uses polypropylene plastics pocket, outer bag to use aluminium foil bag, add a few packet silica-gel desiccants between interior outer bag, To prevent water suction.
Embodiment 5
A kind of feeding composite yeast bacteria additive, be by saccharomycete dry powder, rhizopus dry powder, Trichoderma dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation eggshell of surplus Powder is uniformly mixed, and composite packaging is made:Candida utili bacterium 30000 hundred million, rhizopus 20000 in every 1000g additives A hundred million, Trichoderma 20000 hundred million, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 5g, wintercherry freeze-dried powder 5g, blackcurrant freeze-dried powder 5g, Cactus freeze-dried powder 70g, remaining is fermentation egg-shell meal;Specific production method is the same as embodiment 4.
Embodiment 6
A kind of feeding composite yeast bacteria additive, be by saccharomycete dry powder, rhizopus dry powder, Trichoderma dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation eggshell of surplus Powder is uniformly mixed, and composite packaging is made:Candida tropicalis 40000 hundred million, rhizopus 15000 in every 1000g additives A hundred million, Trichoderma 15000 hundred million, sea-buckthorn freeze-dried powder 30g, Rosa roxburghii freeze-dried powder 12g, wintercherry freeze-dried powder 10g, blackcurrant freeze-dried powder 10g, cactus freeze-dried powder 80g, remaining is fermentation egg-shell meal;Specific production method is the same as embodiment 4.
Embodiment 7
A kind of feeding composite yeast bacteria additive, be by saccharomycete dry powder, rhizopus dry powder, Trichoderma dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation eggshell of surplus Powder is uniformly mixed, and composite packaging is made:Red 60000 hundred million, the saccharomycete in every 1000g additives, rhizopus 8000 hundred million, Trichoderma 8000 hundred million, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 15g, wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 12g, celestial being Freeze-dried powder 90g is slapped, remaining is fermentation egg-shell meal;Specific production method is the same as embodiment 4.
Embodiment 8
A kind of feeding composite yeast bacteria additive, be by saccharomycete dry powder, rhizopus dry powder, Trichoderma dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation eggshell of surplus Powder is uniformly mixed, and composite packaging is made:The brewer's yeast bacterium 50000 hundred million in every 1000g additives, rhizopus 10000 hundred million, Trichoderma 10000 hundred million, sea-buckthorn freeze-dried powder 10g, Rosa roxburghii freeze-dried powder 30g, wintercherry freeze-dried powder 20g, blackcurrant freeze-dried powder 15g, celestial being People slaps freeze-dried powder 100g, remaining is fermentation egg-shell meal;Specific production method is the same as embodiment 4.
Embodiment 9
A kind of feeding composite yeast bacteria additive, be by saccharomycete dry powder, rhizopus dry powder, Trichoderma dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation eggshell of surplus Powder is uniformly mixed, and composite packaging is made:The saccharomyces cerevisiae 55000 hundred million in every 1000g additives, rhizopus 12000 hundred million, Trichoderma 12000 hundred million, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 25g, wintercherry freeze-dried powder 20g, blackcurrant freeze-dried powder 20g, celestial being People slaps freeze-dried powder 100g, remaining is fermentation egg-shell meal;Specific production method is the same as embodiment 4.
Embodiment 10
A kind of feeding composite yeast bacteria additive, be by saccharomycete dry powder, rhizopus dry powder, Trichoderma dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation eggshell of surplus Powder is uniformly mixed, and composite packaging is made:The brewer's yeast bacterium 60000 hundred million in every 1000g additives, rhizopus 20000 hundred million, Trichoderma 20000 hundred million, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 30g, wintercherry freeze-dried powder 30g, blackcurrant freeze-dried powder 30g, celestial being People slaps freeze-dried powder 60g, remaining is fermentation egg-shell meal;Specific production method is the same as embodiment 4.
Embodiment 11
A kind of feeding composite yeast bacteria additive, be by saccharomycete dry powder, rhizopus dry powder, Trichoderma dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation eggshell of surplus Powder is uniformly mixed, and composite packaging is made:Red 45000 hundred million, the saccharomycete in every 1000g additives, rhizopus 25000 hundred million, Trichoderma 25000 hundred million, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 40g, wintercherry freeze-dried powder 25g, blackcurrant freeze-dried powder 25g, celestial being People slaps freeze-dried powder 70g, remaining is fermentation egg-shell meal;Specific production method is the same as embodiment 4.
Embodiment 12
A kind of feeding composite yeast bacteria additive, be by saccharomycete dry powder, rhizopus dry powder, Trichoderma dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the fermentation eggshell of surplus Powder is uniformly mixed, and composite packaging is made:The brewer's yeast bacillus 25000 hundred million in every 1000g additives, rhizopus 30000 Hundred million, Trichoderma 30000 hundred million, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 50g, wintercherry freeze-dried powder 10g, blackcurrant freeze-dried powder 10g, Cactus freeze-dried powder 100g, remaining is fermentation egg-shell meal;Specific production method is the same as embodiment 4.

Claims (10)

1. a kind of feeding composite yeast bacteria additive, it is characterized in that first by saccharomycete, head mold, trichoderma, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt Freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus fermentation egg-shell meal it is mixed It closes uniformly, composite packaging is made:20000 hundred million -60000 hundred million, saccharomycete, head mold 8000 hundred million-in every 1000g additives 30000 hundred million, trichoderma 8000-30000 hundred million, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, Blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-100g, remaining is fermentation egg-shell meal;The sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt Freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are made by following methods, and technical process is to take respectively Fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus remove carpopodium, the impurity such as rotten fruit, rinsing, freezing, lyophilization, low temperature It crushes, does not remove the peel, remove seed step;Rinsing condition described above is the citric acid that weight 0.1% is added with 40 DEG C of water, rinsing 15min, and being stirred continuously, pulls out to be put into pure water and rinses 3 times;Above-mentioned freezing be respectively by sea-buckthorn, Rosa roxburghii Tratt, wintercherry, it is black plus It is cooled to -45 DEG C or less rapidly after logical sequence, cactus sabot;The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, very Reciprocal of duty cycle 120-150Pa is dry until moisture is less than 6%/w;The fermentation egg-shell meal, is to clean up eggshell, dries Moisture, in advance be dried 1-2 hours, be then comminuted into 40-50 mesh powder, be added suitable water make into water content 45-55%/ W, the saccharomycete for adding egg-shell meal weight 20% are mixed thoroughly, are fermented 60-72 hours under the conditions of temperature is 31 DEG C -33 DEG C, fermentation After, fermentation materials are adjusted to temperature and is 82-92 DEG C, carbonyl ammonia is carried out under the conditions of humidity is 80-90% reacts 22-28 days, Then, material is dried to moisture < 5%/w using electric baking case under the conditions of no more than 100 DEG C, through crushing, mistake 100 mesh sieve to obtain the final product.
2. a kind of feeding composite yeast bacteria additive, it is characterized in that first by saccharomycete, head mold, trichoderma, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt Freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus fermentation egg-shell meal it is mixed It closes uniformly, composite packaging is made:30000 hundred million -60000 hundred million, saccharomycete, head mold 10000 hundred million-in every 1000g additives 30000 hundred million, trichoderma 10000 hundred million -30000 hundred million, sea-buckthorn freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder 8-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 60-100g, remaining is fermentation egg-shell meal;The sea-buckthorn freeze-drying Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are made, technical process by following methods To take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus to remove carpopodium, the impurity such as rotten fruit, rinsing, freezing, distillation respectively Dry, low-temperature grinding, is not removed the peel, remove seed step;Rinsing condition described above is the lemon that weight 0.1% is added with 40 DEG C of water Lemon acid, rinses 15min, and is stirred continuously, and pulls out to be put into pure water and rinses 3 times;Above-mentioned freezing be respectively by sea-buckthorn, Rosa roxburghii Tratt, It is cooled to -45 DEG C or less rapidly after wintercherry, blackcurrant, cactus sabot;The process conditions of above-mentioned lyophilization are temperature-22- It is -32 DEG C, vacuum degree 120-150Pa, dry until moisture is less than 6%/w;The fermentation egg-shell meal is that eggshell cleaning is dry Only, moisture is dried, is dried 1-2 hours in advance, is then comminuted into 40-50 mesh powder, suitable water is added makes into water content and exist 45-55%/w, the saccharomycete for adding egg-shell meal weight 20% are mixed thoroughly, and fermentation 60-72 is small under the conditions of temperature is 31 DEG C -33 DEG C When, after fermentation, fermentation materials are adjusted to temperature and is 82-92 DEG C, carries out carbonyl ammonia reaction under the conditions of humidity is 80-90% 22-28 days, then, material is dried to moisture < 5%/w using electric baking case under the conditions of no more than 100 DEG C, passed through It crushes, sieves with 100 mesh sieve to obtain the final product.
3. a kind of feeding composite yeast bacteria additive, it is characterized in that first by saccharomycete, head mold, trichoderma, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt Freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus fermentation egg-shell meal it is mixed It closes uniformly, composite packaging is made:40000 hundred million -60000 hundred million, saccharomycete, head mold 12000 hundred million-in every 1000g additives 30000 hundred million, trichoderma 12000 hundred million -30000 hundred million, sea-buckthorn freeze-dried powder 15-50g, Rosa roxburghii freeze-dried powder 12-50g, wintercherry freeze-drying Powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 70-100g, remaining is fermentation egg-shell meal;The sea-buckthorn freeze-drying Powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are made, technical process by following methods To take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus to remove carpopodium, the impurity such as rotten fruit, rinsing, freezing, distillation respectively Dry, low-temperature grinding, is not removed the peel, remove seed step;Rinsing condition described above is the lemon that weight 0.1% is added with 40 DEG C of water Lemon acid, rinses 15min, and is stirred continuously, and pulls out to be put into pure water and rinses 3 times;Above-mentioned freezing be respectively by sea-buckthorn, Rosa roxburghii Tratt, It is cooled to -45 DEG C or less rapidly after wintercherry, blackcurrant, cactus sabot;The process conditions of above-mentioned lyophilization are temperature-22- It is -32 DEG C, vacuum degree 120-150Pa, dry until moisture is less than 6%/w;The fermentation egg-shell meal is that eggshell cleaning is dry Only, moisture is dried, is dried 1-2 hours in advance, is then comminuted into 40-50 mesh powder, suitable water is added makes into water content and exist 45-55%/w, the saccharomycete for adding egg-shell meal weight 20% are mixed thoroughly, and fermentation 60-72 is small under the conditions of temperature is 31 DEG C -33 DEG C When, after fermentation, fermentation materials are adjusted to temperature and is 82-92 DEG C, carries out carbonyl ammonia reaction under the conditions of humidity is 80-90% 22-28 days, then, material is dried to moisture < 5%/w using electric baking case under the conditions of no more than 100 DEG C, passed through It crushes, sieves with 100 mesh sieve to obtain the final product.
4. a kind of feeding composite yeast bacteria additive, it is characterized in that first by saccharomycete, head mold, trichoderma, sea-buckthorn freeze-dried powder, Rosa roxburghii Tratt Freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add surplus fermentation egg-shell meal it is mixed It closes uniformly, composite packaging is made:50000 hundred million -60000 hundred million, saccharomycete, head mold 15000 hundred million-in every 1000g additives 30000 hundred million, trichoderma 15000 hundred million -30000 hundred million, sea-buckthorn freeze-dried powder 20-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-drying Powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder 80-100g, remaining is fermentation egg-shell meal;The sea-buckthorn is frozen Dry powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are made by following methods, technique mistake Journey is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium, the impurity such as rotten fruit respectively, and rinsing, rises at freezing Magnificent drying, low-temperature grinding are not removed the peel, remove seed step;Rinsing condition described above adds weight 0.1% with 40 DEG C of water Citric acid rinses 15min, and is stirred continuously, and pulls out to be put into pure water and rinses 3 times;Above-mentioned freezing is respectively by sea-buckthorn, thorn It is cooled to -45 DEG C or less rapidly after pears, wintercherry, blackcurrant, cactus sabot;The process conditions of above-mentioned lyophilization are temperature- It is 22-- 32 DEG C, vacuum degree 120-150Pa, dry until moisture is less than 6%/w;The fermentation egg-shell meal is by eggshell Clean up, dry moisture, in advance be dried 1-2 hours, be then comminuted into 40-50 mesh powder, be added suitable water make into containing Water is mixed thoroughly in 45-55%/w, the saccharomycete for adding egg-shell meal weight 20%, is fermented under the conditions of temperature is 31 DEG C -33 DEG C 60-72 hours, after fermentation, fermentation materials is adjusted to temperature and is 82-92 DEG C, carries out carbonyl under the conditions of humidity is 80-90% Ammonia reacts 22-28 days, then, material is dried to moisture < using electric baking case under the conditions of no more than 100 DEG C 5%/w is sieved with 100 mesh sieve through crushing to obtain the final product.
5. a kind of feeding composite yeast bacteria additive as described in claim 1-4, which is characterized in that the saccharomycete is false silk Any one in yeast, red yeast, brewer's yeast, brewer's yeast, fodder yeast.
6. a kind of feeding composite yeast bacteria additive as described in claim 1-4, which is characterized in that the saccharomycete, head mold, Trichoderma is every gram of thalline dry powder product that 200 hundred million are no less than containing viable bacteria concentration.
7. a kind of feeding composite yeast bacteria additive as described in claim 1-4, which is characterized in that the low-temperature grinding is object Material temperature degree is crushed in 0 DEG C or less.
8. a kind of feeding composite yeast bacteria additive as described in claim 1-4, which is characterized in that the composite packaging uses Interior outer bag composite packaging, inner bag use polypropylene plastics pocket, outer packing to use aluminium foil bag, silica dehydrator is filled between interior outer bag Agent pouch.
9. a kind of feeding composite yeast bacteria additive as described in claim 1-4, which is characterized in that the eggshell is egg Any one or a few mixing eggshell in shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell.
10. a kind of feeding composite yeast bacteria additive as described in claim 1-4, which is characterized in that described is dry using heat Air-dry dry or far-infrared ray drying;The drying condition is 180 DEG C -300 DEG C.
CN201810511766.4A 2018-05-25 2018-05-25 A kind of feeding composite yeast bacteria additive Withdrawn CN108576382A (en)

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