CN108567733A - Whitening and anti-aging composition containing dendrobium stem cell extract and application thereof - Google Patents
Whitening and anti-aging composition containing dendrobium stem cell extract and application thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/9783—Angiosperms [Magnoliophyta]
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- A61Q19/08—Anti-ageing preparations
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The invention discloses a whitening anti-aging composition containing a dendrobium stem cell extract and application thereof, wherein the whitening anti-aging composition comprises the following components in parts by weight: 1-3 parts of dendrobium stem cell extract, 10-20 parts of cordyceps militaris extract, 5-15 parts of pawpaw extract and 5-15 parts of salvia miltiorrhiza flower/leaf/root extract. The cordyceps militaris extract, the pawpaw extract and the salvia miltiorrhiza flower/leaf/root extract are compounded with the dendrobium stem cell extract to play a synergistic effect of whitening and removing wrinkles, under the synergistic effect of various extracts, the product can obviously improve the skin quality, improve the skin elasticity, improve the skin proliferation capacity and reduce the skin aging process, the effect is obviously higher than that of singly adding one or two extracts, and the product is safe and non-irritant.
Description
Technical field
The present invention relates to cosmetic technical fields, more particularly to a kind of to contain stem of noble dendrobium stem cell extract whitening anti-aging group
Close object and application thereof.
Background technology
Skin whitening, despeckle beauty treatment, to remain youthful forever, slow down aging be the wonderful dream of people, and people are always thinking various do
Method solves the problems, such as this.With advancing age, the ability of the natural Green Tea Extract of human body, that is, under oxidation resistant ability is continuous
Drop, the cell meeting aging of people, the problems such as also will produce pachylosis, pigmentation in appearance of people, human tissue organ also can
Rapid aging.With China's expanding economy and the improvement of people's living standards, people have higher requirement to quality of life,
Skin whitening, despeckle beauty treatment, remain youthful forever, this world subject that slows down aging it is also of increasing concern in China.
There are many product of whitening or anti-wrinkle on the market at present, be largely be chemically synthesized it is obtained, although in short-term
In can bring effect, but solving the problems, such as at all, and phenomena such as excessive chemical products can lead to skin allergy,
So that skin problem is more serious.
The stem of noble dendrobium is China's rare traditional Chinese medicine, is put into world's endangered protection plant, 76 kinds known to China, the whole world is about
There are 1500 kinds.《Sheng Nong's herbal classic》Deng record, the stem of noble dendrobium has eliminating impediment, lower gas, tonifying five zang organs consumptive disease, by skin heat symptoms caused by an exopathgen, Qiang Yin
And other effects, it has been investigated that the stem of noble dendrobium can significantly improve superoxide dismutase (SOD) level, lipid peroxide (LPO) is reduced, is adjusted
It is horizontal to save brain monoaminergic transmitters, inhibits similar monoamine oxidase (MAO), plays the role of anti-aging.It received in recent years
The dendrobium polysaccharide of concern also has the multiple efficacies such as significant immune-enhancing activity and anti-aging, radioresistance, such as Tiepi Fengdou
The brilliant, stem of noble dendrobium dissipates, peaceful, the beneficial intestines soup of clearing, noble dendrobium stem distillate, stem of noble dendrobium essence, yin-nourishing oral solution and stem of noble dendrobium piece etc..With dendrobium candidum processing
" maple bucket " or " earrings dendrobium ", are referred to as, and " " golden ear dendrobium ", " dendrobium ring ", " Jin Fengdou " are in great demand in Southeast Asia and Europe
It is beautiful.
Plant stem cell (plant stem cell) is undifferentiated cell, has very strong self-renewing and persistently divides
Split ability.Studies have shown that the skin care item containing stem cell can stimulate cell turnover, promote collagen secretion, using dry thin
The effect of born of the same parents is more rapidly more effective than general skin care item.Chinese patent CN 102670438B disclose a kind of anti-aging beauty-care shield
Skin product, containing plant stem cell active matter 0.01~3wt% of 0.001~3wt%, Dragosine carnosine, the plant is dry thin
Cytoactive object is PhytocelltecTMMalusDomestica apples stem cell, PhytocelltecTMSolarVitis grapes
Stem cell, Phytocell tecTMAlp Rose rose leafs stem cell, PhytocelltecTM Argan nuts stem cell,
At least one of RegenistemRiceAp red rice stem cells.The cosmetics and skincare product has improvement skin quality, improves skin elasticity
Effect.
Currently, about stem of noble dendrobium stem cell in skin care item, there are no relevant report, therefore, the present invention selects the stem of noble dendrobium dry
Cell and other extracts compound, and work out not only anti-aging but also while capableing of the composition of whitening.
Invention content
The object of the present invention is to provide a kind of whitening anti-aging composition and its use containing stem of noble dendrobium stem cell extract
On the way, the preferable anti-aging of the anti-apolexis composition goes wrinkle function, while but also with whitening function.
To achieve the above object, the technical solution used in the present invention：A kind of whitening anti-aging composition, including it is following heavy
Measure the ingredient of part：1~3 part of stem of noble dendrobium stem cell extract, 10~20 parts of Cordyceps militaris extract, 5~15 parts of papaya extractives, Radix Salviae Miltiorrhizae
5~15 parts of flower/leaf/root extract.
Single extract is usually used in the prior art and carries out whitening anti-aging, however its effect is ideal not to the utmost, this Shen
Please inventor by numerous studies find, by stem of noble dendrobium stem cell extract, Cordyceps militaris extract, papaya extractives, salvia miltiorrhiza flower/
Leaf/root extract is compounded, and whitening anti-aging significant effect is better than independent extract；And it does not find to use in the prior art
Stem of noble dendrobium stem cell extract is applied in anti-aging skin care product, and there is stem of noble dendrobium stem cell powerful inoxidizability and anti-aging to live
Property, the concentration of the SOD in human plasma can be increased, the ability of oxygen radical is resisted in enhancing, but also can enhance collagen egg
White concentration, fundamentally the problem of the aging of prevention face.
Preferably, whitening anti-aging composition includes the ingredient of following parts by weight：Stem of noble dendrobium stem cell extract 1.5~2.5
Part, 15~20 parts of Cordyceps militaris extract, 10~15 parts of papaya extractives, salvia miltiorrhiza flower/8~15 parts of leaf/root extract.
In the present composition, the proportioning of component can influence the effect of composition, the study found that in the preferred weight
In part range, the whitening antioxidation of the present composition is better.
Preferably, whitening anti-aging composition includes the ingredient of following parts by weight：2 parts of stem of noble dendrobium stem cell extract, pupa worm
16 parts of careless extract, 15 parts of papaya extractives, salvia miltiorrhiza flower/10 parts of leaf/root extract.
Applicant has been surprisingly found that in many experiments, under the specific parts by weight, the whitening antioxygen of the present composition
Change effect and reaches best.
Preferably, the mass ratio of the stem of noble dendrobium stem cell extract and Cordyceps militaris extract is 1:8.
Applicant has been surprisingly found that in many experiments, under the specific weight proportion, the present composition it is anti-oxidant
Effect reaches best.
Preferably, the mass ratio of the stem of noble dendrobium stem cell extract and papaya extractives is 1:7.
Applicant has been surprisingly found that in many experiments, and under the specific quality proportioning, the whitening of the present composition is imitated
Fruit reaches best.
Application of the whitening anti-aging composition in preparing skin care item.
A kind of skin care item of the composition containing whitening anti-aging.
Preferably, mass percent of the whitening anti-aging composition in the skin care item is 1~6%.
Preferably, mass percent of the whitening anti-aging composition in the skin care item is 5%.
Preferably, the skin care item are face cream, lotion, toner, essence dew, facial cleanser.
Whitening anti-aging composition main component and effect used in the present invention are as follows：
The stem of noble dendrobium：It has been investigated that the stem of noble dendrobium can significantly improve superoxide dismutase (SOD) level, lipid peroxide is reduced
(LPO), it is horizontal that brain monoaminergic transmitters are adjusted, inhibits similar monoamine oxidase (MAO), plays the role of anti-aging.Closely
A little dendrobium polysaccharide being concerned in year also have the multiple efficacies such as significant immune-enhancing activity and anti-aging, radioresistance, such as
Capsule Dendrobii, the stem of noble dendrobium dissipate, clearing is peaceful, beneficial intestines soup, noble dendrobium stem distillate, stem of noble dendrobium essence, yin-nourishing oral solution and stem of noble dendrobium piece etc., with dendrobium candidum
" the maple bucket " or " earrings dendrobium " of processing is referred to as " golden ear dendrobium ", " dendrobium ring ", " Jin Fengdou ", is in great demand in Southeast Asia
And it is American-European.
It is raw material that the present invention, which selects the stem cell of the stem of noble dendrobium, and plant stem cell includes about development of plants and to grow all
Formula possesses the cell of eternal life power, is the root (origin) of plant life power.Plant stem cell, which is present in, to be referred to as point
In the special tectonic of raw tissue, there is very surprising power of regeneration.These allow plant constantly to be grown between centuries,
And generate completely new organ.Plant stem cell is the primary raw material and source of skin progenitor cell metabolism, can quickly be activated not
Dormancy stem cell promotes stem cell self-regeneration update, the first viability of reduction epidermal stem cells to keep Skin Cell newborn in a steady stream, from
And reach promotion, firm skin, desalinate the anti-aging effects of wrinkle, while can be easier to be absorbed by the skin the nutrition of plant at
Point；Accelerate human metabolism.
Cordyceps militaris：It is the stroma and larva corpse that section ergot fungus cordyceps sinensis colonizes on Hepialidae insect larvae
Complex, be a kind of traditional rare tonic Chinese herbal medicine material, there is adjusting function of immune system, antitumor, antifatigue, reducing blood lipid etc.
Multiple efficacies.In pharmacology modern study result, cordyceps sinensis contains cordycepic acid about 7%, and carbohydrate 28.9%, fat is about
8.4%, protein about 25%, fat in 82.2% be unsaturated fatty acid, in addition, still contain vitamin B12, ergot lipidol,
Hexose alcohol, alkaloid etc..Cordyceps extract can improve subacute aging mouse red blood cell, and SOD lives in liver and brain
Property, and improve the activity of catalase and glutathione peroxidase in blood.
Pawpaw：It is warm-natured for the dry mature fruit of rosaceous plant chaenomeles lagenaria, sour, Return liver, the spleen channel.With soothing the liver,
The effect of relaxing tendons and activating collaterals, spleen benefiting and stimulating the appetite, analgesic, dispelling wind and eliminating dampness.
Chemical analysis：Pawpaw is containing papain, saponin, flavonoids, oleanolic acid, malic acid, tartaric acid, citric acid, black bearberry
Acid, tannic acid, the ingredients such as vitamin C.
It is anti-oxidant：Papaya powder has stronger antioxidation, can improve hepatocuprein in Patients with Cerebral Infarction blood
(Soo) active, malondialdehyde (MDA) content is reduced, the anti-oxidation efficacy of patients with cerebral ischemic infarction is improved.
Whitening：Studies have shown that papain can inhibit the formation of melanin, the effect of playing freckle removing and whitening.Skin sunburn, sparrow
Spot, chloasma etc. are to be caused by melanin pigmentation, and melanin is substantially the derivative of protein, formed with junket ammonia
The sunlight effect of sour enzyme is related.Research thinks that intracellular tyrosine can form melanin under the catalytic action of enzyme, and
Papain can be such that tyrosinase decomposes, and with the catalytic process for blocking tyrosinase, to reduce the black for forming chloasma
Element is formed.To have the function that eliminating chloasma.
It is anti-inflammatory:Papaya extractives to acetic acid cause mouse peritoneal capillary permeability, mice caused by dimethylbenzene xylene auricle edema and
The formation of swollen hyperplasia of rat granuloma has significant anti-inflammatory effect, can be obviously prolonged the pain threshold of mouse.Inhibit mouse peritoneal
Capillary permeability, dimethylbenzene Mice Auricle and swollen hyperplasia of rat granuloma are formed.
Radix Salviae Miltiorrhizae：Phenolic acid class, chlorophyll in Radix Salviae Miltiorrhizae (SalviamiltiorrhizaBge) etc. have anti-oxidant, removing certainly
By machine, the effect of anti-aging.
The beneficial effects of the present invention are：Stem of noble dendrobium stem cell has powerful inoxidizability and activity of fighting against senium, Ke Yizeng
Add the concentration of the SOD in human plasma, enhancing to resist the ability of oxygen radical, but also the concentration of collagen can be enhanced,
The problem of fundamentally preventing facial aging, while adding with whitening, skin-active, the extraction of oxygen radical Cordyceps militaris can be reduced
Object, papaya extractives, salvia miltiorrhiza flower/leaf/root extract, are compounded with stem of noble dendrobium stem cell extract, play whitening anti-aging
Synergy acts synergistically, and under a variety of extracts synergistic effect, product can be obviously improved skin quality, improve skin elasticity, improve skin
The Reproductive activity of skin reduces the ager process that declines of skin, with obvious effects higher than individually addition one of which or two kinds of extracts, and
And it is safe and non-stimulating.
Specific implementation mode
Each raw material involved by following embodiment is the commercially available universal product unless otherwise instructed.
The preparation method of stem of noble dendrobium stem cell extract of the present invention is：1) good stem of noble dendrobium leaf is chosen, and is cleaned
Disinfection, and it is cut into last shape, manufacture callus；2) callus is inoculated in solid medium and carries out Fiber differentiation, cut more
For the regeneration site of injured tissue to carry out squamous subculture, the product after culture, which be transferred in fluid nutrient medium, is enlarged training
It supports, transfers to bioreactor and carry out large-scale culture, until obtaining the stem of noble dendrobium stem cell suspension of saturation；3) 2) it will obtain in
Stem of noble dendrobium stem cell suspension is obtained, is filtered with 1 μm of filter, is removed culture medium, harvest the cell of retention；And cell is put into -80 DEG C
Superfreeze 2 hours, then take out defrosting 30 minutes, repeat aforesaid operations 5 times, after last time thaw at RT, with super
Sonication instrument smudge cells collect filtrate, in being concentrated in rotating vacuum evaporator after filter filtering, you can obtain stone
Dry measure used in former times stem cell extract.
The making of above-mentioned callus and cultural method and the squamous subculture used expand culture, extensive training
Foster mode is all made of method well known in the art, is not described in detail herein to obtain the stem cell of saturation.
Salvia miltiorrhiza flower/leaf/root extract can be used existing method be prepared or commercially gained；It can also adopt
It is made using the following method；
The preparation of salvia miltiorrhiza flower/leaf/root extract：1) 100~150 days Radix Salviae Miltiorrhizae blades of growth selection or root, flower, with clear
Water is cleaned；2) raw material is mixed and water is added, timing being boiled constantly since liquid is boiled 30~180 minutes；Radix Salviae Miltiorrhizae blade/root/
The mass volume ratio g/ml of flower and water is 0.5~1:10~20；3) stop heating, be cooled to 30~50 DEG C, filtering；
4) after the filtrate that step 3) obtains is filtered again, through vacuum filtration, that is, activity extract is prepared.
A kind of whitening anti-aging composition containing stem of noble dendrobium stem cell extract, includes the ingredient of following parts by weight：The stem of noble dendrobium
1~3 part of stem cell extract, 10~20 parts of Cordyceps militaris extract, 5~15 parts of papaya extractives, salvia miltiorrhiza flower/leaf/root extract 5
~15 parts.
The composition of the present invention can be by by stem of noble dendrobium stem cell extract, Cordyceps militaris extract, papaya extractives, Radix Salviae Miltiorrhizae
Flower/leaf/root extract is mixed to get by the parts by weight.
Embodiment 1
The whitening anti-aging composition of the present embodiment, includes the ingredient of following parts by weight：Stem of noble dendrobium stem cell extract 1.5
Part, 15 parts of Cordyceps militaris extract, 10 parts of papaya extractives, salvia miltiorrhiza flower/8 parts of leaf/root extract.
Above-mentioned whitening anti-aging composition is applied to cream, and cream includes the ingredient of following mass percent：Whitening is anti-
Anti-aging composition 5%, jojoba oil 8%, stearine 3%, cetostearyl alcohol 3%, PEG-10 stearates 2%, thoroughly
Bright matter acid sodium 0.15%, carbomer 0.5%, potassium sorbate 0.1%, surplus is deionized water.
1) jojoba oil, stearine, cetostearyl alcohol, PEG-100 stearates are mixed and is heated to
80 DEG C, keep the temperature 20 minutes；2) Sodium Hyaluronate, carbomer, deionized water, potassium sorbate are mixed and are heated to 80 DEG C,
Heat preservation 20 minutes；3) mixture in step 2) being added in step 1), homogeneous stirring, 80 DEG C keep the temperature 10 minutes, are cooled to 55 DEG C,
Whitening anti-aging composition is added, is cooled to 45 DEG C, is cooled to 35 DEG C, discharging is filling to obtain cream.
Embodiment 2
The whitening anti-aging composition of the present embodiment, includes the ingredient of following parts by weight：1 part of stem of noble dendrobium stem cell extract,
15 parts of Cordyceps militaris extract, 5 parts of papaya extractives, salvia miltiorrhiza flower/5 parts of leaf/root extract.
The whitening anti-aging composition of the present embodiment is applied in cream, wherein the quality percentage of whitening anti-aging combination
Than being 6%, remaining ingredient and content and cream preparation method are same as Example 1.
Embodiment 3
The whitening anti-aging composition of the present embodiment, includes the ingredient of following parts by weight：2 parts of stem of noble dendrobium stem cell extract,
16 parts of Cordyceps militaris extract, 15 parts of papaya extractives, salvia miltiorrhiza flower/10 parts of leaf/root extract.
It is used to prepare moisturizing emulsion using the composition, moisturizing emulsion includes following mass percent ingredient:Containing above-mentioned
Whitening anti-aging composition 5%, stearine 3%, mine ester 2.5%, dimethyl silicone polymer 6%, glycerine 5%, the third two
Alcohol 5%, dormant oil 5%, 1,3-BDO 20.0%, carbomer 0.5%, triethanolamine 0.5%, potassium sorbate 0.5%, surplus are
Deionized water.
The preparation method of the moisturizing emulsion, includes the following steps, 1) first use 1,3-BDO and portions of de-ionized water to dissolve
Stem of noble dendrobium stem cell extract, Cordyceps militaris extract, papaya extractives, salvia miltiorrhiza flower/leaf/root extract, filtering are for use；2) by mine
Oil, mine ester, stearine, propylene glycol, 75 DEG C -80 DEG C of dimethyl silicone polymer stir evenly；3) remaining deionized water, sweet
Oil, carbomer, 75 DEG C -80 DEG C of potassium sorbate stir evenly；4) by step 2) with 3) made from two kinds of mixtures be successively pumped into breast
Change pot, triethanolamine is added in homogeneous 3 minutes；5) stirring is cooled to 40 DEG C, and filtered stem of noble dendrobium stem cell extract, pupa is added
Cordyceps, papaya extractives, salvia miltiorrhiza flower/leaf/root extract, stir evenly, are cooled to room temperature.
Embodiment 4
The whitening anti-aging composition of the present embodiment includes the ingredient of following parts by weight, stem of noble dendrobium stem cell extract 2.5
Part, 20 parts of Cordyceps militaris extract, 12 parts of papaya extractives, salvia miltiorrhiza flower/15 parts of leaf/root extract.
The present embodiment is applied to prepare in moisturizing emulsion, and difference from Example 3 is, wherein whitening anti-aging combines
The mass percent of object is 1%.
Embodiment 5
The whitening anti-aging composition of the present embodiment, includes the ingredient of following parts by weight：2 parts of stem of noble dendrobium stem cell extract,
10 parts of Cordyceps militaris extract, 15 parts of papaya extractives, salvia miltiorrhiza flower/12 parts of leaf/root extract.
The present embodiment whitening anti-aging is applied in moisturizing emulsion, and difference from Example 3 is, wherein above-mentioned
The mass percent of whitening anti-aging composition is 5%.
Embodiment 6
The whitening anti-aging composition of the present embodiment, includes the ingredient of following parts by weight：3 parts of stem of noble dendrobium stem cell extract,
18 parts of Cordyceps militaris extract, 15 parts of papaya extractives, salvia miltiorrhiza flower/12 parts of leaf/root extract.
The present embodiment is applied in moisturizing emulsion, and difference from Example 3 is, wherein whitening anti-aging composition
Mass percent is 5%.
Comparative example 1
The whitening anti-aging composition of this comparative example, includes the ingredient of following parts by weight：16 parts of Cordyceps militaris extract, pawpaw
15 parts of extract, salvia miltiorrhiza flower/10 parts of leaf/root extract.
Comparative example 2
The whitening anti-aging composition of this comparative example, includes the ingredient of following parts by weight：2 parts of stem of noble dendrobium stem cell extract,
15 parts of papaya extractives, salvia miltiorrhiza flower/10 parts of leaf/root extract.
Comparative example 3
The whitening anti-aging composition of this comparative example, includes the ingredient of following parts by weight：2 parts of stem of noble dendrobium stem cell extract,
16 parts of Cordyceps militaris extract, salvia miltiorrhiza flower/10 parts of leaf/root extract.
Comparative example 4
The whitening anti-aging composition of this comparative example, includes the ingredient of following parts by weight：15 parts of papaya extractives, Radix Salviae Miltiorrhizae
10 parts of flower/leaf/root extract.
Comparative example 5
The whitening anti-aging composition of this comparative example, includes the ingredient of following parts by weight：16 parts of Cordyceps militaris extract, Radix Salviae Miltiorrhizae
10 parts of flower/leaf/root extract.
Comparative example 6
The whitening anti-aging composition of this comparative example, includes the ingredient of following parts by weight：5 parts of stem of noble dendrobium stem cell extract,
16 parts of Cordyceps militaris extract, 15 parts of papaya extractives, salvia miltiorrhiza flower/10 parts of leaf/root extract.
Comparative example 7
The whitening anti-aging composition of this comparative example, includes the ingredient of following parts by weight：2 parts of stem of noble dendrobium stem cell extract,
8 parts of Cordyceps militaris extract, 15 parts of papaya extractives, salvia miltiorrhiza flower/10 parts of leaf/root extract.
Effect example 1
1~6 senile-resistant efficacy evaluation test of the embodiment of the present invention
To the measurement of Hydroxyl Radical Scavenging, experimental method is carried out according to the method that following document provides：Lin Xiangcheng, soup
Spring, Luo Yanghe；The extraction of general flavone and antioxidant activity research [J] guangdong agricultural sciences in rhizoma homalonemae, 2012,5:96-98；
With reference to Fenton reaction methods, H2O2OH is generated after being mixed with ferrous ion, there is OH very high reaction to live
Property, the time-to-live is short, but salicylic acid is added in the reaction system, can effectively capture OH and generate color products, the production
Object has very strong absorption in 536nm, if the extract for having and removing OH functions is added, and can compete OH with salicylic acid,
To make the production quantity of purple product reduce, using set time reaction method, measure at 536nm containing the present composition and
The absorbance of the composition of comparative example, and being compared with blank solution, then can measured object to the Scavenging activity of OH, calculate public
Formula：OH clearance rates (%)=100 (A0-Ax)/A0× 100%.
Experimental method：The 2mmol/L FeSO in 25ml colorimetric cylinders43ml、1mmol/L H2O23ml shakes up, and is subsequently added into
6mmol/L salicylic acid 3ml, shake up, and are taken out after 37 DEG C of heating water bath 15min, survey its absorbance A0.Then a certain concentration is added
Prepare liquid 0.6ml, then add distilled water 0.4ml, shake up, continue heating water bath 15min, take out survey its absorbance Ax。
For after elimination plus total 1.0ml prepare liquids and distilled water caused by system absorbance value reduction, method is same as above, constant temperature
Its absorbance value A is surveyed after 15min00, add 1ml distilled water, survey its primary absorbance A after shaking up againxx, AIt reduces=A00-Axx。
Prepare liquid is to OH clearance rates：OH clearance rates (%)=100 (A0-Ax-AIt reduces)/A0
Above-mentioned prepare liquid is the composition of Examples 1 to 6 and comparative example 1~7.Obtain table 1；
Table 1
As can be seen from Table 1, stem of noble dendrobium stem cell extract, Cordyceps militaris extract, papaya extractives, salvia miltiorrhiza flower/leaf/root carry
Object is taken to all have senile-resistant efficacy.Four all have senile-resistant efficacy, wherein papaya extractives, salvia miltiorrhiza flower/leaf/root extract two
Person's compounding is better than Cordyceps militaris extract, salvia miltiorrhiza flower/leaf/root extract compounding effect, and four to compound its senile-resistant efficacy best.
Stem of noble dendrobium stem cell extract, Cordyceps militaris extract, papaya extractives, salvia miltiorrhiza flower/leaf/root extraction in comparative example 6,7
The parts by weight of compositions are not complete within the scope of the present invention, it can be seen that its effect is not as good as the present invention, it is seen that stem of noble dendrobium stem cell carries
Take object, Cordyceps militaris extract, papaya extractives, salvia miltiorrhiza flower/four kinds of leaf/root extract extract by parts by weight group of the present invention
Be combined, keep the antioxidant effect of skin care item of the present invention more preferable, also, as shown in Table 1, when 2 parts of stem of noble dendrobium stem cell extract,
When 16 parts of Cordyceps militaris extract, 15 parts of papaya extractives, salvia miltiorrhiza flower/10 parts of leaf/root extract, skin care item of the present invention it is anti-oxidant
Effect is best, this is that applicant has been surprisingly found that.
Effect example 2
The whitening anti-aging composition of the present invention not only has the effect of anti-aging.Also there is white-skinned face function, this effect simultaneously
Fruit example has investigated stem of noble dendrobium stem cell extract and the mass ratio of papaya extractives imitates whitening anti-aging composition whitening of the present invention
The influence of fruit.In order to investigate the influence, we are prepared for different compositions according to the method for embodiment 1.Each experimental group combination
Object is made of following components in parts by weight, and 1~3 part of stem of noble dendrobium stem cell extract, 10~20 parts of Cordyceps militaris extract, pawpaw carry
Take 5~15 parts of object, salvia miltiorrhiza flower/5~15 parts of leaf/root extract；The wherein quality of stem of noble dendrobium stem cell extract and papaya extractives
Than as shown in table 2 below.
Table 2
Test method：
Tyrosinase inhibition rate
Tyrosinase is a kind of polyphenol oxidase, is key enzyme, rate-limiting enzyme in dermal melanin forming process, tyrosine
The activity of enzyme is proportionate with B16 cell amount, and activity change can cause dyschromicum skin disease.Tyrosinase inhibits
Agent prevents melanin from being formed, can be used to prevent and treat the diseases such as pigmentation by inhibiting tyrosinase activity.
Experiment material：L-tyrosine solution (0.5mg/ml), tyrosinase (100U/ml) method (are appointed with reference to the prior art
Clearly, gorgeous etc. in knowing, enzyme microb whitening anti-aging efficacy study, fragrance flavor and cosmetic, 3 phases in 2008) measure above-mentioned reality
The tyrosinase inhibition rate for testing group 1~4, obtains table 3.
2.2.2 experimental result
Table 3
| Experimental group | Tyrosinase inhibition rate (%) |
| Experimental group 1 | 85.6% |
| Experimental group 2 | 83.4% |
| Experimental group 3 | 82.7% |
| Experimental group 4 | 73.4% |
By table 3 as it can be seen that the skin care item tyrosinase inhibition rate highest of experimental group 1, it is seen then that stem of noble dendrobium stem cell extract and wood
The weight ratio of the weight ratio of melon extract is 1:When 7, the effect of skin care item whitening of the present invention reaches best.
Guinea pig skin stimulation test
0.5g Examples 1 to 6 whitening anti-aging compositions are respectively taken, being diluted to 100g using deionized water is configured to test sample
Product.Given the test agent using skin irritation test method (《Cosmetics health specification》2007 editions toxicology test methods) it is surveyed
Examination.
Experimental animal：Cavy weight 250g~300g.
Experimental result：Through 14 days Skin Irritation Tests, stimulate the reaction is had no.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is protected to the present invention
The limitation of range is protected, although being explained in detail to the present invention with reference to preferred embodiment, those skilled in the art should
Understand, technical scheme of the present invention can be modified or replaced equivalently, without departing from the essence of technical solution of the present invention
And range.
Claims (10)
1. a kind of whitening anti-aging composition, which is characterized in that include the ingredient of following parts by weight：Stem of noble dendrobium stem cell extract 1
~3 parts, 10~20 parts of Cordyceps militaris extract, 5~15 parts of papaya extractives, salvia miltiorrhiza flower/5~15 parts of leaf/root extract.
2. whitening anti-aging composition according to claim 1, which is characterized in that include the ingredient of following parts by weight：Stone
1.5~2.5 parts of dry measure used in former times stem cell extract, 15~20 parts of Cordyceps militaris extract, 10~15 parts of papaya extractives, salvia miltiorrhiza flower/leaf/root
8~15 parts of extract.
3. whitening anti-aging composition according to claim 1, which is characterized in that include the ingredient of following parts by weight：Stone
2 parts of dry measure used in former times stem cell extract, 16 parts of Cordyceps militaris extract, 15 parts of papaya extractives, salvia miltiorrhiza flower/10 parts of leaf/root extract.
4. whitening anti-aging composition according to claim 1, which is characterized in that the stem of noble dendrobium stem cell extract and pupa
The mass ratio of Cordyceps is 1:8.
5. whitening anti-aging composition according to claim 1, which is characterized in that the stem of noble dendrobium stem cell extract and wood
The mass ratio of melon extract is 1:7.
6. according to application of any one of Claims 1 to 5 whitening anti-aging composition in preparing skin care item.
7. a kind of skin care item containing the whitening anti-aging composition as described in any one of Claims 1 to 5.
8. skin care item according to claim 7, which is characterized in that the whitening anti-aging composition is in the skin care item
Mass percent be 1~6%.
9. skin care item according to claim 7, which is characterized in that the whitening anti-aging composition is in the skin care item
Mass percent be 5%.
10. application of the whitening anti-aging composition according to claim 6 in preparing skin care item, which is characterized in that institute
It is face cream, lotion, toner, essence dew, facial cleanser to state skin care item.
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| CN113662892A (en) * | 2021-08-12 | 2021-11-19 | 楚香(上海)生物科技有限公司 | Anti-aging regulatory protein plant extraction activator and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107823107A (en) * | 2017-11-30 | 2018-03-23 | 广州赛莱拉干细胞科技股份有限公司 | A kind of anti-apolexis composition containing dried lily bulb cell extract and its application in skin care item |
| CN107961209A (en) * | 2017-12-04 | 2018-04-27 | 广州赛莱拉干细胞科技股份有限公司 | A kind of skin care item of whitening anti-aging and preparation method thereof |
| CN108030760A (en) * | 2017-12-08 | 2018-05-15 | 广州赛莱拉干细胞科技股份有限公司 | A kind of lightening compositions containing radix astragali stem cell extract and its application |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107823107A (en) * | 2017-11-30 | 2018-03-23 | 广州赛莱拉干细胞科技股份有限公司 | A kind of anti-apolexis composition containing dried lily bulb cell extract and its application in skin care item |
| CN107961209A (en) * | 2017-12-04 | 2018-04-27 | 广州赛莱拉干细胞科技股份有限公司 | A kind of skin care item of whitening anti-aging and preparation method thereof |
| CN108030760A (en) * | 2017-12-08 | 2018-05-15 | 广州赛莱拉干细胞科技股份有限公司 | A kind of lightening compositions containing radix astragali stem cell extract and its application |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113662892A (en) * | 2021-08-12 | 2021-11-19 | 楚香(上海)生物科技有限公司 | Anti-aging regulatory protein plant extraction activator and preparation method thereof |
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