CN108524804A

CN108524804A - A kind of Chinese medicine composition for treating pharyngo-laryngitis chronica

Info

Publication number: CN108524804A
Application number: CN201810663850.8A
Authority: CN
Inventors: 吴文喜; 吴创平
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-06-25
Filing date: 2018-06-25
Publication date: 2018-09-14

Abstract

The invention belongs to field of pharmaceutical technology, and in particular to a kind of Chinese medicine composition for treating pharyngo-laryngitis chronica, including following raw material are prepared：Puncturevine caltrop fruit, semen astragali complanati, smilax, paris polyphylla, radix paeoniae rubra, Radix Paeoniae Alba, Longstamen Onion Bulb, Snakegourd Fruit, Radix Rehmanniae, the root of straight ladybell, Radix Ophiopogonis, the root bark of white mulberry, root of Chinese trichosanthes, honeysuckle, Fructus Forsythiae, lophatherum gracile, campanulaceae, peppermint, Radix Glycyrrhizae, the stem of noble dendrobium, Chinese globeflower, the bletilla striata, asparagus fern, radix scrophulariae, rhizoma atractylodis, cordyceps sinensis, Rhizoma Atractylodis Macrocephalae.The application formula is three burnt unimpeded with being reconciled to medicine, reaches in vivo functionality benign cycle, comforts sun, a kind of of Eradicates heresy special effects treats pharyngo-laryngitis chronica, takes concentrated traditional Chinese medicine ball orally, therapeutic effect is significantly, it can be achieved that treating both manifestation and root cause of disease, securely and reliably, without any side effects.

Description

A kind of Chinese medicine composition for treating pharyngo-laryngitis chronica

【Technical field】

The invention belongs to field of pharmaceutical technology, and in particular to a kind of Chinese medicine composition for treating pharyngo-laryngitis chronica.

【Background technology】

Pharyngo-laryngitis chronica is the common disease of ENT ＆ HN Surgery Dept., and Different types of etiopathogenises is mainly shown as pharyngeal dry pain, foreign body sensation, sound Mute, pharyngeal discomfort sense, toposcopy have occupying the minority for abnormal change, most patients to have acute laryngopharyngitis medical history, mental element to account for Certain proportion eats overdrying, heat, hard food etc..Differential diagnosis in tcm has several sayings, has kidney water deficiency, flaring up of deficient fire, disappear bright lung It is cloudy, smoked burn throat caused by, treated with nourishiing yin to clear away the lung-heat nourishing yin and lessening fire.Have to invade lung system by damp and hot heresy and lose and control, misdiagnosis or sends out repeatedly Make, damage yin-fluid causes the deficiency of Yin, body fluid deficiency aqueous that cannot wet one's whistle, therefore its this disease is the deficiency of Yin, since constitution difference has void

It disturbs and causes on sun.Up to the present, preferable drug and therapy clinically there is no to effect a radical cure this disease, many patients are repeatedly To each hospital admission, the drug applied in the market has that drug price is higher mostly, the unsatisfactory disadvantage of therapeutic effect.

Ancestor, which cures the disease, to be good at dialectical, is good at medication, faces card as just before going into battle, medication is such as used military forces, it is necessary to shrewd syndrome, careful group in detail Side, medical knowledge is not known in flexible medication, i.e., difficult dialectical, dialectical unknown, has no way of making laws, and causes to pile up flavour of a drug then, disorderly and unsystematic, Xian Renkai When prescription, usually double medicines and book play synergistic effect person during the compatibility application of two medicine of implied meaning, have and mutually disappear that it is secondary with specially taking institute Elder has interaction to generate special-effect, is all referred to as to medicine；It is drug in pairs, really many small sides that surface, which is seen, With recipe essence, therefore ancestor uses it to medicine；Entitled medication actually uses side, medicine to be different from materia medica, because it has simply to learning Compatibility, from being pharmaceutically raised to prescription, without going research medicine to learning, then prescription oneself would not be removed, also absolute place Bad side illustrates the function to medicine, rather convenient in Clinical practice.Dialectical and medication is to compatibility, and effect of curing the disease is double, Chinese medicine generation Family is all kind to be caused with to unimpeded three coke of medicine, human body major part disease is all that three cokes are obstructed, and three cokes are that human-body viscera function is unimpeded heavy Channel is wanted, if viscera function is not smooth, vivotoxin can not be just discharged from stool, urine, skin, just will produce phlegm in vivo Turbid and negative and positive are not parallel.Part of the body cavity above the diaphragm housing the heart and lungs cardiopulmonary, middle burnt taste, kidney of lower warmer bladder, therefore the target burnt according to unobstructed three, the applicant match 5 prescriptions transfer cardiopulmonary, taste, kidney bladder according to first medication of curing the disease, and act synergistically, compatibility good toxin expelling work(turbid to clear up internal phlegm Can, to resist treatment disease, promote to treat pharyngo-laryngitis chronica effect, quickly and effectively.It cures the disease first dialectical, finds the root of the trouble under the verification of source Medication reconciliation viscera function cooperation resists the disease medicine again.Up to the present, clinical still quickly to be effected a radical cure without a kind of drug This disease.They produce and the use of the Chinese patent drug function for the treatment of pharyngo-laryngitis chronica are mainly antibacterial, analgesic, anti-inflammatory, with this medicine To treat pharyngo-laryngitis chronica, belongs to and control table and do not control root, so being unable to reach treatment optimum efficiency.

【Invention content】

For the present invention for compatibility is unable to using drug using treatment pharyngo-laryngitis chronica institute at present, therapeutic scheme is not comprehensive enough, controls table Root is not controlled, place that there are many deficiencies, and a kind of formula handed down from one's ancestors by several generations' clinical verification is provided, with to three coke of medicine reconciliation It is unimpeded, reach in vivo functionality benign cycle, comforts sun, a kind of of Eradicates heresy special effects treats pharyngo-laryngitis chronica, concentrated traditional Chinese medicine for oral administration Ball, therapeutic effect is significantly, it can be achieved that treating both manifestation and root cause of disease, securely and reliably, without any side effects.

To achieve the goals above, the technical solution adopted by the present invention is as follows：

A kind of Chinese medicine composition for treating pharyngo-laryngitis chronica includes 1-8 parts of puncturevine caltrop fruit, semen astragali complanati 0.5-6 in parts by weight Part, 1-8 parts of smilax, 0.5-9 parts of paris polyphylla, 1-8 parts of radix paeoniae rubra, 0.5-6 parts of Radix Paeoniae Alba, 1-7 parts of Longstamen Onion Bulb, 0.5-7 parts of Snakegourd Fruit, Radix Rehmanniae 1- 9 parts, 1-7 parts of the root of straight ladybell, Radix Ophiopogonis 0.5-6 part, 0.5-7 parts of the root bark of white mulberry, 1-6 parts of root of Chinese trichosanthes, 0.5-8 parts of honeysuckle, 0.5-8 parts of Fructus Forsythiae, 1-7 parts of lophatherum gracile, 0.5-7 parts of campanulaceae, 0.5-6 parts of peppermint, 0.5-6 parts of Radix Glycyrrhizae, 0.5-6 parts of the stem of noble dendrobium, 1-7 parts of Chinese globeflower, the bletilla striata 0.5-7 parts, 1-7 parts of asparagus fern, 0.5-7 parts of radix scrophulariae, 0.5-8 parts of rhizoma atractylodis, 0.01-3 parts of cordyceps sinensis, 1-5 parts of Rhizoma Atractylodis Macrocephalae.

Preferably, the Chinese medicine composition is prepared including following raw material in parts by weight：Puncturevine caltrop fruit 1.5- 5.5 parts, 1-4 parts of semen astragali complanati, 2-6 parts of smilax, 1-7 parts of paris polyphylla, 2-5 parts of radix paeoniae rubra, 1.5-4 parts of Radix Paeoniae Alba, 2-5 parts of Longstamen Onion Bulb, Snakegourd Fruit 1.5-5 parts, 2-6 parts of Radix Rehmanniae, 2-4 parts of the root of straight ladybell, Radix Ophiopogonis 1.5-4 part, 1.5-5 parts of the root bark of white mulberry, 2-5 parts of root of Chinese trichosanthes, 2-6 parts of honeysuckle, 2-5 parts of Fructus Forsythiae, 2-5 parts of lophatherum gracile, 2-5 parts of campanulaceae, 1-4 parts of peppermint, 1-3 parts of Radix Glycyrrhizae, 1-3 parts of the stem of noble dendrobium, 2-5 parts of Chinese globeflower, in vain 1.5-3.5 parts of splendid achnatherum, 1.5-4.5 parts of asparagus fern, 1.5-4 parts of radix scrophulariae, 1.5-4 parts of rhizoma atractylodis, 0.5-2 parts of cordyceps sinensis, Rhizoma Atractylodis Macrocephalae 1.5-3.5 Part.

It is further preferred that the Chinese medicine composition, is prepared including following raw material in parts by weight：Puncturevine caltrop fruit 2.5 parts, 2 parts of semen astragali complanati, 3 parts of smilax, 2 parts of paris polyphylla, 3 parts of radix paeoniae rubra, 2 parts of Radix Paeoniae Alba, 3 parts of Longstamen Onion Bulb, 2 parts of Snakegourd Fruit, 3 parts of Radix Rehmanniae, sand 3 parts of ginseng, 2 parts of Radix Ophiopogonis, 3 parts of the root bark of white mulberry, 3 parts of root of Chinese trichosanthes, 3.5 parts of honeysuckle, 3.5 parts of Fructus Forsythiae, 4 parts of lophatherum gracile, 3 parts of campanulaceae, peppermint 2 Part, 2 parts of Radix Glycyrrhizae, 2 parts of the stem of noble dendrobium, 3 parts of Chinese globeflower, 2.5 parts of the bletilla striata, 3 parts of asparagus fern, 3 parts of radix scrophulariae, 2.5 parts of rhizoma atractylodis, 1 part of cordyceps sinensis, 2.5 parts of Rhizoma Atractylodis Macrocephalae.

It further illustrates, above-mentioned raw material is：After the dry fruit of puncturevine caltrop fruit is carried out ultramicro grinding, mass concentration, which is added, is The ethanol solution of 75-85% carries out refluxing extraction 3-5 times, and each extraction time is 5-10 minutes, will extract filtered filter every time After liquid merges, it is concentrated into 1.11-1.12 times of relative density, you can obtain puncturevine caltrop fruit extract, main 4.5- containing flavone compound 5.1%, saponins compound 54.25-59.17 μ g/g, alkaloid 50.26-61.04mg/g, protein 1.65-2.14mg/g；

The seed of semen astragali complanati is carried out after being crushed, 5-10 times of aqueous solution progress water is added and carries, the temperature that water carries is 50-60 DEG C, is carried The time taken is 30-40 minutes, and water uses the ultrasonic wave that power is 100W to carry out assisted extraction, the filtrate after water is carried during carrying It is filtered, obtained Semen Astragali Complanati extract master contains flavonoids 6.24-7.31mg/g, alkaloid 2.15-2.47mg/g, triterpene Substance 1.58-2.31mg/g；

The 75wt% ethanol solutions that 3-5 times of volume is added after smilax is crushed extract 0.2-0.3 hours, filter to take filtrate, Filter residue repeats to extract, and merges 3 filtrate, you can obtain smilax extracting solution, the main 30.25-39.47mg/g of substance containing saponin, Succinic acid 1.69-2.38mg/g, palmitic acid 1.15-2.04mg/g, Quercetin 1.21-1.57mg/g；

The fresh quench stem of paris polyphylla is blended using blender, 5-8 times of water is subsequently added into and carries out Soxhlet extraction 1-2 hours, is collected Filtrate, by filtrate according to flow velocity be 0.1-0.2m/s by the Filter column containing polyether sulfone after, collect extracting solution, you can obtain flea Not extracting solution, the main 27.36-35.62mg/g of substance containing saponin, Flavonoid substances 15.36-21.04mg/g；

8-10 times of 65wt% ethanol solution is added after the rhizome of radix paeoniae rubra is crushed to extract at 60 DEG C 3 times, each extraction time It is 1-2 hours, the extracting solution extracted every time is subjected to ethyl alcohol recycling, concentration is dried to obtain red paeonia extract, main total containing Chinese herbaceous peony Glycosides 18.36-25.26mg/g, tannin class 8.12-10.68mg/g, flavonoids 6.26-8.36mg/g, volatile oil 3.25- 6.04mg/g；

After moisture content≤5% is dried in the rhizome of Radix Paeoniae Alba, 100 mesh sieve is smashed it through, by remaining large particulate matter after continued powder Broken to sieve with 100 mesh sieve, it is 1 according to volume ratio that will then filter powder with distilled water:It is carried out in 10 ratio input refluxing reactor Refluxing extraction 2-4 times, it is 1-2 hours each, filtrate is obtained after filtering, filtrate is spray-dried, and obtains radix paeoniae alba extraction, it is main Contain glycoside substance 20.36-29.21mg/g, Flavonoid substances 21.34-28.18mg/g, polysaccharide 105.36-124.30mg/g；

Longstamen Onion Bulb is added in diatomite and carries out mortar grinder into pureed, is subsequently added into 5-10 times of 75wt% ethanol solution at 60 DEG C It extracts 3 times, filters, merging filtrate, 5-10 times of 30wt% acetum, which is added, in filtrate carries out overnight precipitation extraction, filtering Take filtrate, you can obtain longstamen onion bulb extract, main A containing macrostemonoside 5.36-6.54mg/g, macrostemonoside D 1.26-3.20mg/g, Chinese onion White glycosides E 1.02-2.08mg/g, macrostemonoside F 1.12-2.64mg/g；

By the kernel of Snakegourd Fruit, pericarp according to weight ratio 1:Be put into steam copper and accumulated after 1 hybrid slicing, steam from lower part to The temperature of top injection heating, steam is 160-230 DEG C, and the high temperature mixing steam of boiling is directly transported by rising appendix Into the cooling tube of decline, collects with liquefied pot after being-4-- 2 DEG C cooling in temperature, condensed after repressurization liquefaction To substance in mainly 15.63-23.45mg/g containing flavonoid glycoside, triterpenoid saponin 25.36-31.05mg/g, protein 15.3- 20.6%, organic acid 10.6-13.5%, carbohydrate 12.63-21.30%, alkaloid 8.65-9.78% chemical compositions；

By after the cooking of Radix Rehmanniae, stirring is slurried, and water is added, is gradually added into 5-10 times of 50wt% acetum and stirs into a paste, It is subsequently added into water, the hydrolysis of 50wt% acetums mixing standing 15 hours, three layers of filtered through gauze is crossed, is incorporated as filtrate after filtering The activated carbon of filtrate total volume 0.1-0.2%, cleans, and can be obtained Radix Rehmanniae extracting solution after filtering, wherein paste, water, The volume ratio of 50wt% acetums is 1:2-3:8-10；By detecting Radix Rehmanniae extracting solution master 8.6-11.2% containing martynoside, dihydro Catalpol glycosides 2.6-3.1%, aucubin 1.2-2.8%, acteoside 0.9-1.6%, Catalpol glycosides 1.3-2.4%；

The root of straight ladybell rhizome is added to 10-12 times of water to decoct 1-2 times, it is 30-40 minutes each, merging filtrate is filtered, and depressurize while hot It is 1.01-1.02 times to be concentrated into relative density, is subsequently added into 75wt% ethanol solutions and is stirred, and is to be carried out at -5 DEG C in temperature Precipitation and separation takes supernatant, and ethanol solution is recovered by filtration, be concentrated into relative density be 1.02-1.10 times after, carry out spraying do It is dry, Radix Adenophorae (Radix Glehniae) extract is obtained, by detection, main 11.2-14.6% containing triterpenoid saponin and starch 21.6-30.5%；

It after cleaning Radix Ophiopogonis, is blended using blender, is subsequently added into 5-10 times of water and carries out refluxing extraction 3 times, each 1-2 is small When, filtering filtrate will merge three times, and after drying is concentrated into 1.01 times of original volume, 70-80 wt% ethanol solutions are added in-1-0 DEG C Lower progress alcohol extracting 5-8 hours, centrifugation take precipitation spray drying to obtain Radix Ophiopogonis activity extract, main to contain Radix Ophiopogonis by detection Saponin A 21.03-28.14mg/g, ophiopogonin B 30.21-39.54mg/g, ophiopogonone A 18.69-25.37mg/g；

It after the root skin of the root bark of white mulberry is dried, crushes, 70-80 wt% ethanol solutions is added and carry out refluxing extraction 3-5 times under room temperature, return Each 20-30 minutes of stream extraction, filters to take filtrate, obtains medicinal extract after being concentrated into 1.01-1.02 times of original volume while hot, medicinal extract is made Deproteinized is removed with Sevage methods, takes supernatant, after cellulase, absolute ethyl alcohol and stirring is added in supernatant, precipitates 10 hours, connects It and uses centrifuge 2000r/min centrifugal treatings 3-5 minutes, be filtered under diminished pressure taking precipitate and obtain mulberry bark extract, by inspection Survey main 60.2-74.6mg/g containing flavone compound；Wherein supernatant：Cellulase：The volume ratio of absolute ethyl alcohol is 2-3:1: 5-8；

Root of Chinese trichosanthes be added in 10-12 times of water and is decocted, decoction divides three phases, 50-60 DEG C of small fire to decoct 20-30 Minute, 80-90 DEG C of moderate heat decocts 10-20 minutes, and 50-60 DEG C of small fire decocts 10-20 minutes, and filtrate is then obtained by filtration, Absolute ethyl alcohol progress alcohol extracting is added to stay overnight, centrifuging and taking filtrate obtains root of Chinese trichosanthes extracting solution, by detection, mainly contains polysaccharide Substance 31.02-35.61mg/g, protein 15.68-20.17mg/g, saponin(e 10.24-13.54mg/g and amino acid 8.29- 11.37mg/g active ingredients；

The flower of honeysuckle is put into high temperature and pressure sealer, with steam pressure to 6-10 kilograms of pressure, temperature at 120 degree to 180 degree, Under the action of centrifugal rotation, the effective ingredient in Chinese medicine is separated in solution by 2-4 hours steam pressures, waits for natural cooling Afterwards, solution is put into freeze drier using micro computer control technology and is freeze-dried, obtain Flos Lonicerae extract, by inspection Survey mainly contains terpene 50.3-68.1mg/g, flavonoids 61.3-75.4mg/g, organic acid 15.6-24.7mg/g, ferro element 38.85-45.21 μ g/g, calcium constituent 30.21-38.69 μ g/g, Zn-ef ficiency 1.05-1.87 μ g/g, manganese element 6.21-7.50 μ g/ g；

It is put into high temperature and pressure sealer after the fruit of Fructus Forsythiae is crushed, with steam pressure to 8-12 kilograms of pressure, temperature is in 160- 200 °, under the action of centrifugal rotation, the effective ingredient in Chinese medicine is separated in solution by 2-4 hours steam pressures, waits for nature After cooling, solution is put into freeze drier using micro computer control technology and is freeze-dried, forsythia suspense extraction is obtained, passed through Cross the main 18.6-25.8mg/g containing flavonoids of detection, forsythin 62.3-71.4mg/g, Forsythinol glycosides C 9.5-12.4mg/g, Fructus Forsythiae Alcohol glycosides D 10.3-12.3mg/g；

6-7 times of water is added after lophatherum gracile is crushed, temperature is to be stirred 10-20 minutes at 50-60 DEG C, stands overnight, then heats Starting to stir after to 100 DEG C, constant temperature stirs 2 hours, and centrifugal filtration while hot takes filtrate, filter residue is repeated the above steps 2-3 times, Merging filtrate can be obtained henon bamboo leaf extract, by the main 1.59-2.15% containing general flavone of retrieval, polysaccharide 15.69-18.64%, egg White matter 13.6-15.2%, ferro element 56.36-72.06 μ g/g, calcium constituent 36.89-48.68 μ g/g, Zn-ef ficiency 1.26-2.64 μ g/ G, manganese element 6.02-8.38 μ g/g；

The rhizome of campanulaceae is extracted by SCF-CO 2 equipment, extract liquor is obtained, is subsequently added into 70-80 wt% second Alcoholic solution alcohol extracting 2-3 hours, centrifugal filtration take filtrate, are concentrated under reduced pressure up to no ethyl alcohol taste, are diluted with water to 0.8g/ crude drugs Ml, by macroporous resin column, washed resin is then eluted using 70-80 wt% ethanol solutions to clarifying, and eluent carries out It is dried under reduced pressure after reduced pressure, obtains Radix Platycodonis extract, contain total saposins 9.21-11.16% by retrieval is main；

It is put into steam copper and accumulates after the rhizome of peppermint is sliced, steam is sprayed from lower to upper part to be heated, the temperature of steam Degree is 180-200 DEG C, the high temperature mixing steam of boiling is delivered directly to by rising appendix in the cooling tube of decline, Yu Wen It spends to be collected after-4-- 2 DEG C of coolings with liquefied pot, repressurization is obtained in the substance that condensation obtains after liquefying mainly containing left-handed Menthol 70.2-78.5%, isomenthone 2.15-3.07%, pulegone 1.59-2.07%, peppermint isoflavone aglycone 3.05- 5.12mg/g, Rosmarinic acid 12.02-21.08mg/g；

It crushed 20 mesh sieve after the rhizome of Radix Glycyrrhizae is dried in the shade, weigh 1g, petroleum ether is added and flows back 2 hours and dries in the shade, is subsequently added into 65- 75wt% ethanol solution refluxing extractions, the temperature of extraction is 50-60 DEG C, the time of extraction is 30-40 minutes, and extraction is obtained by filtration Liquid is concentrated to dryness, and obtains licorice, by detection, mainly contains triterpenoid saponin 3.54-4.25 μ g/g and flavonoids Compound 112.03-134.26 μ g/g；

It is put into steam copper and accumulates after the rhizome of the stem of noble dendrobium is sliced, steam is sprayed from lower to upper part to be heated, the temperature of steam Degree is 180-200 DEG C, the high temperature mixing steam of boiling is delivered directly to by rising appendix in the cooling tube of decline, Yu Wen It obtains condensing after degree is-5-- 3 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains the stem of noble dendrobium in obtained substance Polysaccharide 124.55-134.26mg/g, dendrobine 26.45-29.34mg/g, amino acid 1 10.21-120.12mg/g, ketone chemical combination Object 34.23-38.04mg/g；

After Chinese globeflower removal of impurities, cleaning, 20 mesh sieve is crushed, is then soaked using the brine that water diluted concentration is 2-5mol/l Bubble 20-30 minutes, is then extracted 15-20 minutes under 30-50kpa pressure, to cooling under normal pressure after extraction, filters to take filtrate, It is concentrated under reduced pressure into 1.02-1.05 times of relative density and can be obtained Chinese globeflower extract, by the main 35.68- containing alkaloid of detection 54.12mg/g, Flavonoids 98.65-115.21mg/g, vitexin 32.26-41.95mg/g and orientoside 30.24- 38.18mg/g；

By the rhizome of the bletilla striata, after crushing sieves with 100 mesh sieve, 5-10 times of water is added, while it is 250,000 IU/g that suitable enzyme activity, which is added, Cellulase carry out refluxing extraction 3-5 time, merging filtrate, by filtrate addition 55-65wt% ethanol solutions, stirring is positioned over Temperature is to stand 10-18 hours under subzero 5-2 degree, takes supernatant to recycle ethanol solution, bletilla striata extract is obtained after low temperature drying, Contain bletilla polysaccharide 79.54-82.35% by detection is main；

The rhizome of asparagus fern is added after 10-12 times of water and impregnates 20-30 minutes, then 50-60 DEG C of small fire decoction 20-30 minutes, 70-80 DEG C of moderate heat decocts 10-15 minutes, closes natural cooling after fire, and then 90-100 DEG C of big fire decocts 10-20 minutes again, repeats 3 Filtrate is filtered to take after secondary and can be obtained asparagus fern extracting solution, and amino acid 245.62-261.24mg/g, polysaccharide are mainly contained by detection 154.85-194.68mg/g with saponins compound 15.02-17.14mg/g；

The rhizome of radix scrophulariae is smashed it through into 20 mesh sieve, the 55-65wt% ethanol solutions that 10-12 times of volume is added extract It 10-30 minutes, uses power to carry out assisted extraction for the ultrasonic wave of 300-400W during extraction, filtrate is filtered to take, by filter residue weight Multiple above-mentioned steps alcohol extracting 2-3 times, merging filtrate, by the spraying that inlet temperature is 200-300 DEG C, outlet temperature is 70-80 DEG C Drying can be obtained Radix Scrophulariae extract in drier, by detecting main 75.15-78.14% containing harpagide, harpagoside 2.56-3.14%, radix scrophulariae kind glycosides 1.24-1.65%；

It is put into steam copper and accumulates after the rhizome of rhizoma atractylodis is sliced, steam is sprayed from lower to upper part to be heated, the temperature of steam Degree is 180-200 DEG C, the high temperature mixing steam of boiling is delivered directly to by rising appendix in the cooling tube of decline, Yu Wen After degree is-4-- 2 DEG C cooling in collection and liquefied pot, obtain condensing after repressurization liquefaction mainly main containing terpene in obtained substance Class compound 11.25-13.15mg/g, polysaccharide 50.20-64.35mg/g, glucosides 89.23-97.24mg/g, amino acid 6.15- 7.24%, mineral element 1.05-2.36%；

After the rhizome crushing of cordyceps sinensis is sieved with 100 mesh sieve, 5-10 times of water is added, while it is 250,000 that suitable enzyme activity, which is added, The cellulase of IU/g carries out refluxing extraction 3-5 times, merging filtrate, 55-65wt% ethanol solutions is added in filtrate, stirring is put It is to stand 10-18 hours under subzero 5-2 degree to be placed in temperature, takes supernatant to recycle ethanol solution, is mainly lived after low temperature drying Property ingredient be Cordyceps sinensis polysaccharide 38.12-41.05mg/g；

The rhizome of Rhizoma Atractylodis Macrocephalae is added after 10-12 times of water and impregnates 20-30 minutes, then 50-60 DEG C of small fire decoction 20-30 minutes, 70-80 DEG C of moderate heat decocts 10-15 minutes, closes natural cooling after fire, and then 90-100 DEG C of big fire decocts 10-20 minutes again, repeats 3 Filtrate is filtered to take after secondary and can be obtained Rhizoma Atractylodis Macrocephalae extracting solution, by detecting main main 34.17-39.58mg/g containing polysaccharide, amino acid 8.12-10.24mg/g, flavones 10.26-14.58mg/g.

A kind of Chinese drugs agentia of Chinese medicine composition that treating pharyngo-laryngitis chronica is by the Chinese medicine group obtained by claim 1 Object is closed to be made plus common auxiliary material in pharmacy.

The dosage form of Chinese drugs agentia as described above is oral solution, tablet, capsule or pill.

Under be classified as the drug effect of raw material use herein：

Tribulus terrestris is annual herb, and stem is lieed down by base portion branch, filbert.It is 1.5-5 centimetres long, leaflet 6-14, to life, square Circle, 6-15 millimeters long, 2-5 millimeters wide, the sharp point in top or blunt, base portion slightly deflection, subcircular, full edge.Semen astragali complanati is planted for pulse family The mature seed of object astragatus complanatus.Nature and flavor：Sweet, warm.Channel tropism：Enter Liver Channel, kidney channel.It invigorates the kidney and stops nocturnal emission, nourishes the liver to improve visual acuity, tonifying liver, benefit Kidney, improving eyesight, controlling nocturnal emission with astringent drugs.Smilax, Chinese medicine name.For the dry rhizome of liliaceous plant smilacis glabra, climbed for perennial evergreen Shape shrub is born in hillside or hayashishita more.Be used as medicine part select its dry after rhizome.It is sweet in flavor, light, it is mild-natured.There is removing toxic substances, dehumidify, The effect of easing joint movement.Chinese medicine paris polyphylla be liliaceous plant paris polyphylla, RHIZOMA PARIDIS and its several congeners rhizome. Its function has：Clearing heat and detoxicating, antiasthmatic-antitussive, relieve dizziness, high fever, infantile convulsions, epilepsy, etc. arresting convulsion, detumescence hemostasis.It cures mainly：Carbuncle swells, furunculosis, scrofula, larynx numbness, chronic gas Guan Yan, child convulsion twitch, snakebite and bugbite, women's irregular menstruation, traumatic injury.Radix paeoniae rubra, Chinese medicine name.For ranunculaceae plant radix paeoniae rubra Or the dry root of river radix paeoniae rubra.Bitter is slightly cold.Return liver warp.There is the effect of clearing heat and cooling blood, promoting blood circulation.Radix Paeoniae Alba, Chinese medicine name.For hair The dry root of gelsemium section plant peony Paeonia tacti lora Pall..With nourishing blood for regulating menstruation, astringing YIN to stop sweating, soft liver relieves pain, The effect of suppressing liver-YANG.Longstamen Onion Bulb, Latin literary fame Allium macrostemon Bunge. belong to Liliaceae, allium.Acrid flavour, Hardship, it is warm-natured.The thoughts of returning home, lung, stomach, large intestine channel.Activate yang and remove abstruction, promoting the circulation of qi lead it is stagnant.Snakegourd Fruit, it is perennial to climb type herbaceous plant.Property slight bitter, It is cold, sweet in flavor, return lung, stomach, large intestine channel.With clearing away heat and eliminating phlegm, relieving stuffiness of the chest by dispersing aggregation of pathogens, moisturize laxation improves systema cariovasculare functional, eliminating the phlegm It is cough-relieving, antibacterial, antiulcer, antitumor and rush down inferior pharmacological activity.Radix Rehmanniae is the root tuber of scrophulariaceae rehmannia glutinosa plant.It is sweet in flavor, bitter, It is cold.Enter the heart, liver and kidney channel.Clearing heat and cooling blood, yin-nourishing are promoted the production of body fluid.The root of straight ladybell, Chinese medicine name.For adenophora tetraphylla Adenophora The root of tetraphylla (Thunb.) Fisch. plants.With replenishing the vital essence and removing heat, the effect of moistening lung for removing phlegm, reinforcing stomach reg fluid.Taste Sweet, slight bitter, cold nature.It attaches to the lung and stomach meridians.Radix Ophiopogonis perennial herb, small root tuber are Chinese medicine, there is the benefits of quench one's thirst of promoting the production of body fluid, moisten the lung and relieve the cough； Cold nature, sweet in flavor, slight bitter.Thoughts of returning home warp, lung channel, stomach.The root bark of white mulberry, Chinese medicine name.For the root of moraceae plants mulberry Morus alba L. Skin.It is distributed in all parts of the country.With removing heat from lung and relieving asthma, the effect of inducing diuresis for removing edema.It is usually used in dyspnea and cough due to lung-heat, water drink stops lung, turgor asthma It is anxious, oedema, tinea pedis, difficult urination.It is sweet in flavor, pungent, it is cold in nature.Return lung, the spleen channel.Removing heat from lung and relieving asthma, inducing diuresis for removing edema.Root of Chinese trichosanthes is Curcurbitaceae The root of plant Chinese juniper beach wormwood is a kind of Chinese medicine, is clearing heat-fire class drug, specific effect is clearing heat-fire, is promoted the production of body fluid to quench thirst, apocenosis disappears It is swollen.Sweet in flavor, slight bitter, cold nature.It attaches to the lung and stomach meridians.Honeysuckle, Chinese medicine name；Property is sweet, cold；It attaches to the lung and stomach meridians.With clearing heat and detoxicating, anti-inflammatory The effect of detumescence.Fructus Forsythiae, cold nature, bitter.Distributed in lung channel, the heart channel of Hang-Shaoyin, small intestinl channel.Clearing heat and detoxicating, dispersing swelling and dissipating binds.Lophatherum gracile, gas is micro-, It is lightly seasoned.It is cold in nature, it is sweet in flavor, light.Thoughts of returning home warp, stomach, small intestinl channel；Clearing heat and relieving fidgetness, diuresis；Clearing heat and relieving fidgetness, diuresis.Campanulaceae, it is cylindrical Or spindle, it slightly distorts, occasionally there is branch, 6-25 centimetres long, 0.5-2.5 centimetres of diameter.It is used as medicine root.Gas is micro-, after taste is slightly sweet slightly Hardship, it is mild-natured, it is bitter, pungent；It is distributed in lung channel；Clearing lung and eliminating the phlegm, relieving sore throat and evacuating pus.Peppermint, cool in nature, acrid flavour；Distributed in lung channel, Liver Channel；Dispelling wind-heat, Refresh oneself, promoting eruption.Radix Glycyrrhizae is a kind of help Chinese herbal medicine；Medicinal part is root and rhizome, and property is sweet, flat, enters the heart, lung, spleen, stomach； Invigorate the spleen and benefit qi, expelling phlegm and arresting coughing, relieving spasm to stop pain, clearing heat and detoxicating, coordinating the drug actions of a prescription.The stem of noble dendrobium is in elongated cylindrical, odorless, lightly seasoned.Property is micro- It is cold, it is sweet in flavor；Return stomach, kidney channel；Reinforcing stomach reg fluid, nourishing Yin and clearing heat.Trollflower, scientific name：Trollius chinensis Bunge are The plant of Ranunculaceae Trollius；Can be clearing heat and detoxicating, cure mainly tonsillitis, tympanitis；It is acute for acute and chronic tonsillitis Tympanitis, acute myringitis, acute conjunctivitis, acute lymphangitis.The bletilla striata（Scientific name：Bletilla striata）Bitter, it is sweet, It is puckery, cold in nature, return lung, stomach；Liver Channel.There are astringing to arrest bleeding, the work(of detumescence and promoting granulation, for all cards of interior external hemorrhage and carbuncle swells, scald, brothers Chap, anal fissure etc..Asparagus fern is usually used in dryness of the lung dry cough, and pertussis phlegm is glutinous, soreness of waist and knee joint, osteopyrexia and fever, Heat Diabetes, pyreticosis Tianjin wound, Dry throat and mouth, dry constipation of intestines.It is sweet in flavor, bitter, it is cold in nature；Return lung, kidney channel.Nourishing yin to moisten dryness, clearing lung-heat are promoted the production of body fluid.Radix scrophulariae, cold nature, sweet in flavor, It is bitter, salty.Distributed in lung channel, stomach, kidney channel.Cool blood enriching yin, purging intense heat and detonicating.Rhizoma atractylodis, warm-natured, acrid flavour, hardship.Converge to the spleen channel, stomach, Liver Channel. Drying damp and strengthening spleen, expelling wind and clearing away cold, improving eyesight.Pharmacological activity mainly plays the role of digestive system, anti-inflammatory effect, hypoglycemic effect, anti-lacks Oxygen effect, antibiosis and antiviral functions, hepatoprotective effect, effect, antiarrhythmic effect etc. to nervous system.Cordyceps sinensis is wheat Angle Cordycepps fungi cordyceps sinensis colonizes in the complex of stroma and larva corpse on Hepialidae insect larvae, mild-natured, sweet in flavor. Distributed in lung channel, kidney channel.Kidney-reinforcing Yang-strengthening, tonifying lung are relievingd asthma, hemostasis and phlegm, are had and are adjusted the multiple efficacies such as function of immune system, antifatigue. Rhizoma Atractylodis Macrocephalae, Classification system Atractylodes macrocephala.It is used as medicine with rhizome, there is multinomial medicinal function.Rhizoma Atractylodis Macrocephalae bitter, It is sweet, it is warm-natured.Returns spleen, stomach.With strengthening the spleen and replenishing qi, the effect of eliminate dampness and have diuretic effect, hidroschesis, tocolysis, it is used for spleen eating less, abdominal distension to be let out It rushes down, phlegm retention anti-dazzle nervous, oedema, spontaneous perspiration, fetal irritability.

It is to extract each raw material to obtain corresponding active constituent, and it is further to pass through the applicant in the application The content of certain ingredients in research extraction prescription in the active constituent of 27 taste medicines reaches for use after certain proportion, then uses normal Each raw material is prepared into corresponding dosage form by the technological means of rule.Have compared to relatively directly dosage form is prepared into using active compound More preferable curative effect.

In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：

The applicant refers to modern pharmacological research achievement on the basis of studying Traditional Chinese Medicine Coryza Treated by Syndrome Differentiation, by examination for many years Test, multiple formulations screened, 27 taste medicine collective effects of full side complement each other, existing cold and cool product in side, also have warm nature it Medicine, using medicines of both cold and hot natures simultaneously, existing tonifying speen and tonifying kidney, clearing heat and detoxicating medicine, the product for also having diuresis, quenching one's thirst, mutually assistant system cooperate, should Prescription compatibility is proper, has used 11 pairs of medicines burnt with monarch drug in a prescription compatibility, unimpeded three, has promoted viscera function cooperation to resist the disease, disappear Except old complaint, pharmacodynamic feature is greatly improved, rapid healing is reached to pharyngo-laryngitis chronica disease, prepares and is easy, therapeutic effect is notable, can Realize treating both manifestation and root cause of disease, it is securely and reliably, without any side effects.

【Specific implementation mode】

Many details are elaborated in the following description in order to fully understand the present invention.But the present invention can be with very much Implement different from other manner described here, those skilled in the art can do without violating the connotation of the present invention It is similar to improve, therefore the present invention is not limited to the specific embodiments disclosed below.

Embodiment 1：

A kind of Chinese medicine composition for treating pharyngo-laryngitis chronica includes 1 part of puncturevine caltrop fruit, 0.5 part of semen astragali complanati, soil in parts by weight 1 part of Poria cocos, 0.5 part of paris polyphylla, 1 part of radix paeoniae rubra, 0.5 part of Radix Paeoniae Alba, 1 part of Longstamen Onion Bulb, 0.5 part of Snakegourd Fruit, 1 part of Radix Rehmanniae, 1 part of the root of straight ladybell, Radix Ophiopogonis 0.5 Part, 0.5 part of the root bark of white mulberry, 1 part of root of Chinese trichosanthes, 0.5 part of honeysuckle, 0.5 part of Fructus Forsythiae, 1 part of lophatherum gracile, 0.5 part of campanulaceae, 0.5 part of peppermint are sweet 0.5 part of grass, 0.5 part of the stem of noble dendrobium, 1 part of Chinese globeflower, 0.5 part of the bletilla striata, 1 part of asparagus fern, 0.5 part of radix scrophulariae, 0.5 part of rhizoma atractylodis, cordyceps sinensis 0.01 part, 1 part of Rhizoma Atractylodis Macrocephalae.

Further illustrate that above-mentioned is that the ethyl alcohol that mass concentration is 75% is added after the dry fruit of puncturevine caltrop fruit is carried out ultramicro grinding Solution carries out refluxing extraction 3 times, and each extraction time is 5 minutes, after extracting filtered filtrate merging every time, is concentrated into phase To 1.11 times of density, you can obtain puncturevine caltrop fruit extract, it is main containing flavone compound 4.5%, 54.25 μ g/g of saponins compound, Alkaloid 50.26mg/g, protein 1.65mg/g.

The seed of semen astragali complanati is carried out after being crushed, 5 times of aqueous solutions progress water are added and carry, the temperature that water carries is 50 DEG C, extraction Time be 30 minutes, water using the ultrasonic wave that power is 100W carries out assisted extraction during carrying, and filtrate after water is carried carries out Filtering, obtained Semen Astragali Complanati extract master contain flavonoids 6.24mg/g, alkaloid 2.15mg/g, triterpene substance 1.58mg/ g。

The 75wt% ethanol solutions that 3 times of volumes are added after the crushing of smilax are extracted 0.2 hour, filtrate is filtered to take, Filter residue repeats to extract, and merges 3 filtrate, you can obtains smilax extracting solution, the main 30.25mg/g of substance containing saponin, succinic acid 1.69mg/g, palmitic acid 1.15mg/g, Quercetin 1.21mg/g.

The fresh quench stem of paris polyphylla is blended using blender, 5 times of water is subsequently added into and carries out Soxhlet extraction 1 hour, is collected Filtrate, by filtrate according to flow velocity be 0.1m/s by the Filter column containing polyether sulfone after, collect extracting solution, you can obtain paris polyphylla and carry Take liquid, the main 27.36mg/g of substance containing saponin, Flavonoid substances 15.36mg/g.

8 times of 65wt% ethanol solutions are added after the rhizome of radix paeoniae rubra is crushed to extract at 60 DEG C 3 times, every time when extraction Between be 1 hour, the extracting solution extracted every time is subjected to ethyl alcohol recycling, concentration is dried to obtain red paeonia extract, main total containing Chinese herbaceous peony Glycosides 18.36mg/g, tannin class 8.12mg/g, flavonoids 6.26mg/g, volatile oil 3.25mg/g.

After moisture content≤5% is dried in the rhizome of Radix Paeoniae Alba, smash it through 100 mesh sieve, by remaining large particulate matter after Continued powder is broken to be sieved with 100 mesh sieve, and it is 1 according to volume ratio that will then filter powder with distilled water:In 10 ratio input refluxing reactor It carries out refluxing extraction 2 times, 1 hour every time, filtrate is obtained after filtering, filtrate is spray-dried, obtain radix paeoniae alba extraction, it is main Contain glycoside substance 20.36mg/g, Flavonoid substances 21.34mg/g, polysaccharide 105.36mg/g.

Longstamen Onion Bulb is added in diatomite and carries out mortar grinder into pureed, is subsequently added into 5 times of 75wt% ethanol solutions at 60 DEG C It extracts 3 times, filters, merging filtrate, 5 times of 30wt% acetums, which are added, in filtrate carries out overnight precipitation extraction, filters to take filter Liquid, you can obtain longstamen onion bulb extract, main A containing macrostemonoside 5.36mg/g, macrostemonoside D 1.26mg/g, macrostemonoside E 1.02mg/g, Macrostemonoside F 1.12mg/g.

By the kernel of Snakegourd Fruit, pericarp according to weight ratio 1:It is put into steam copper and is accumulated after 1 hybrid slicing, steam is under Portion is sprayed to top heats, and the temperature of steam is 160 DEG C, and the high temperature mixing steam of boiling is directly transported by rising appendix Into the cooling tube of decline, is collected with liquefied pot after being -4 DEG C cooling in temperature, obtain what condensation obtained after repressurization liquefaction Mainly 15.63mg/g containing flavonoid glycoside, triterpenoid saponin 25.36mg/g, protein 15.3%, organic acid 10.6%, carbohydrate in substance 12.63%, 8.65% chemical composition of alkaloid.

After Radix Rehmanniae is cooked, stirring is slurried, and water is added, is gradually added into 5 times of 50wt% acetums and stirs into a paste, It is subsequently added into water, the hydrolysis of 50wt% acetums mixing standing 15 hours, three layers of filtered through gauze is crossed, is incorporated as filtrate after filtering The activated carbon of filtrate total volume 0.1%, cleans, and Radix Rehmanniae extracting solution, wherein paste, water, 50wt% vinegar are can be obtained after filtering The volume ratio of acid solution is 1:2:8；By detecting Radix Rehmanniae extracting solution master containing martynoside 8.6%, dihydro Catalpol glycosides 2.6%, peach leaf coral Coral glycosides 1.2%, acteoside 0.9%, Catalpol glycosides 1.3%.

The root of straight ladybell rhizome is added to 10 times of water to decoct 1 time, 30 minutes every time, filters merging filtrate, and be concentrated under reduced pressure while hot It is 1.01 times to relative density, is subsequently added into 75wt% ethanol solutions and is stirred, is to carry out precipitation and separation at -5 DEG C in temperature, Take supernatant, ethanol solution be recovered by filtration, be concentrated into relative density be 1.02 times after, be spray-dried, obtain the root of straight ladybell extraction Object, it is main to contain triterpenoid saponin 11.2% and starch 21.6% by detection.

It after cleaning Radix Ophiopogonis, is blended using blender, is subsequently added into 5 times of water and carries out refluxing extraction 3 times, 1 is small every time When, filtering filtrate will merge three times, after drying is concentrated into 1.01 times of original volume, 70 wt% ethanol solutions be added and are carried out at -1 DEG C Alcohol extracting 5 hours, centrifugation take precipitation spray drying to obtain Radix Ophiopogonis activity extract, main to contain Ophiopogonin A by detection 21.03mg/g, ophiopogonin B 30.21mg/g, ophiopogonone A 18.69mg/g.

It after the root skin of the root bark of white mulberry is dried, crushes, 70 wt% ethanol solutions is added and carry out refluxing extraction 3 times under room temperature, return Stream extraction 20 minutes every time, filters to take filtrate, medicinal extract is obtained after being concentrated into 1.01 times of original volume while hot, and medicinal extract is used Sevage Method removes deproteinized, takes supernatant, and cellulase is added, after absolute ethyl alcohol and stirring in supernatant, is precipitated 10 hours, then use from Scheming 2000r/min centrifugal treatings 3 minutes, are filtered under diminished pressure taking precipitate and obtain mulberry bark extract, contain flavones by detection is main Class compound 60.2mg/g；Wherein supernatant：Cellulase：The volume ratio of absolute ethyl alcohol is 2:1:5,

Root of Chinese trichosanthes be added in 10 times of water and is decocted, decoction divides three phases, 50 DEG C of small fire to decoct 20 minutes, 80 DEG C Moderate heat decoct 10 minutes, 50 DEG C of small fire decocts 10 minutes, and filtrate is then obtained by filtration, and absolute ethyl alcohol is added and carries out alcohol extracting Night, centrifuging and taking filtrate obtain root of Chinese trichosanthes extracting solution, by detection, mainly contain polysaccharose substance 31.02mg/g, protein 15.68mg/g, saponin(e 10.24mg/g and amino acid 8.29mg/g active ingredients.

The flower of honeysuckle is put into high temperature and pressure sealer, with steam pressure to 6 kilograms of pressure, temperature is at 120 degree to 180 Degree, under the action of centrifugal rotation, is separated to the effective ingredient in Chinese medicine in solution by 2 hours steam pressures, waits for natural cooling Afterwards, solution is put into freeze drier using micro computer control technology and is freeze-dried, obtain Flos Lonicerae extract, by inspection Survey mainly contains terpene 50.3mg/g, flavonoids 61.3mg/g, organic acid 15.6mg/g, 38.85 μ g/g of ferro element, calcium constituent 30.21 μ g/g, 1.05 μ g/g of Zn-ef ficiency, 6.21 μ g/g of manganese element.

It is put into high temperature and pressure sealer after the fruit of Fructus Forsythiae is crushed, with steam pressure to 8 kilograms of pressure, temperature exists 160 °, under the action of centrifugal rotation, the effective ingredient in Chinese medicine is separated in solution by 2 hours steam pressures, waits for that nature is cold But after, solution is put into freeze drier using micro computer control technology and is freeze-dried, forsythia suspense extraction is obtained, passed through The main 18.6mg/g containing flavonoids of detection, forsythin 62.3mg/g, Forsythinol glycosides C 9.5mg/g, Forsythinol glycosides D 10.3mg/g.

6 times of water are added after lophatherum gracile is crushed, temperature is to be stirred 10 minutes at 50 DEG C, stands overnight, is then heated to 100 Start to stir after DEG C, constant temperature stirs 2 hours, and centrifugal filtration while hot takes filtrate, and filter residue is repeated the above steps 2 times, merging filtrate Henon bamboo leaf extract is can be obtained, it is main containing general flavone 1.59%, polysaccharide 15.69%, protein 13.6%, ferro element by retrieval 56.36 μ g/g, 36.89 μ g/g of calcium constituent, 1.26 μ g/g of Zn-ef ficiency, 6.02 μ g/g of manganese element.

The rhizome of campanulaceae is passed through into supercritical CO₂Extraction equipment is extracted, and extract liquor is obtained, and is subsequently added into 70 wt% second Alcoholic solution alcohol extracting 2 hours, centrifugal filtration take filtrate, are concentrated under reduced pressure up to no ethyl alcohol taste, are diluted with water to 0.8g/ crude drug ml, By macroporous resin column, washed resin is then eluted using 70 wt% ethanol solutions, eluent depressurize dense to clarifying It is dried under reduced pressure after contracting, obtains Radix Platycodonis extract, contain total saposins 9.21% by retrieval is main.

It is put into steam copper and accumulates after the rhizome of peppermint is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 180 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen It obtains condensing after degree is -4 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains levorotatory menthol in obtained substance 70.2%, isomenthone 2.15%, pulegone 1.59%, peppermint isoflavone aglycone 3.05mg/g, Rosmarinic acid 12.02mg/g.

It crushed 20 mesh sieve after the rhizome of Radix Glycyrrhizae is dried in the shade, weigh 1g, petroleum ether is added and flows back 2 hours and dries in the shade, then adds Enter 65wt% ethanol solution refluxing extractions, the temperature of extraction is 50 DEG C, the time of extraction is 30 minutes, and extracting solution is obtained by filtration and subtracts Pressure is concentrated to dryness, and obtains licorice, by detection, mainly contains 3.54 μ g/g of triterpenoid saponin and flavone compound 112.03μg/g。

It is put into steam copper and accumulates after the rhizome of the stem of noble dendrobium is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 180 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen It obtains condensing after degree is -5 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains dendrobium polysaccharide in obtained substance 124.55mg/g, dendrobine 26.45mg/g, amino acid 1 10.21mg/g, ketone compounds 34.23mg/g.

After Chinese globeflower removal of impurities, cleaning, 20 mesh sieve is crushed, the brine that water diluted concentration is 2mol/l is then used to carry out It impregnates 20 minutes, is then extracted 15 minutes under 30kpa pressure, to cooling under normal pressure after extraction, filter to take filtrate, be concentrated under reduced pressure Chinese globeflower extract is can be obtained again to relative density 1.02, by the main 35.68mg/g containing alkaloid of detection, total flavonoid chemical combination Object 98.65mg/g, vitexin 32.26mg/g and orientoside 30.24mg/g.

By the rhizome of the bletilla striata, after crushing sieves with 100 mesh sieve, 5 times of water are added, while it is 250,000 IU/g that suitable enzyme activity, which is added, Cellulase carry out refluxing extraction 3 times, 55wt% ethanol solutions are added in filtrate by merging filtrate, and stirring is positioned over temperature and is 10 hours are stood under subzero 5 degree, takes supernatant to recycle ethanol solution, bletilla striata extract is obtained after low temperature drying, is led by detection Contain bletilla polysaccharide 79.54%.

The rhizome of asparagus fern is added after 10 times of water and impregnates 20 minutes, then 50 DEG C of small fires decoctions 20 minutes, 70 DEG C of moderate heats It decocts 10 minutes, closes natural cooling after fire, then 90 DEG C of big fire decoct 10 minutes again, and filtrate is filtered to take after being repeated 3 times to be obtained To asparagus fern extracting solution, amino acid 245.62mg/g, polysaccharide 154.85mg/g and saponins compound are mainly contained by detection 15.02mg/g。

The rhizome of radix scrophulariae is smashed it through into 20 mesh sieve, the 55wt% ethanol solutions that 10 times of volumes are added extract 10 Minute, it uses power to carry out assisted extraction for the ultrasonic wave of 300W during extraction, filters to take filtrate, filter residue is repeated the above steps Alcohol extracting 2 times, merging filtrate can be obtained by drying in inlet temperature is 200 DEG C, outlet temperature is 70 DEG C spray dryer Radix Scrophulariae extract, it is main containing harpagide 75.15%, harpagoside 2.56%, radix scrophulariae kind glycosides 1.24% by detecting.

It is put into steam copper and accumulates after the rhizome of rhizoma atractylodis is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 180 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen After degree is -4 DEG C cooling in collection and liquefied pot, obtain condensing after repressurization liquefaction mainly main containing terpenoid in obtained substance Object 11.25mg/g, polysaccharide 50.20mg/g, glucosides 89.23mg/g, amino acid 6.15%, mineral element 1.05%.

After the rhizome crushing of cordyceps sinensis is sieved with 100 mesh sieve, 5 times of water are added, while it is 250,000 that suitable enzyme activity, which is added, The cellulase of IU/g carries out refluxing extraction 3 times, merging filtrate, 55wt% ethanol solutions is added in filtrate, stirring is positioned over temperature Degree is to stand 10 hours under subzero 5 degree, and supernatant is taken to recycle ethanol solution, and it is cordyceps sinensis that main active is obtained after low temperature drying Polysaccharide 38.12mg/g

The rhizome of Rhizoma Atractylodis Macrocephalae is added after 10 times of water and impregnates 20 minutes, then 50 DEG C of small fires decoct 20 minutes, 70 DEG C of moderate heats decoctions 10 minutes, close natural cooling after fire, then 90 DEG C of big fire decoct 10 minutes again, filtered to take after being repeated 3 times filtrate can be obtained it is white Art extracting solution, by detecting main main 34.17mg/g containing polysaccharide, amino acid 8.12mg/g, flavones 10.26mg/g.Preparation method： Oral solution is made in above-mentioned raw materials addition auxiliary material, you can take.Oral for patient, 2 times a day, each 10mL, 14 are one The course for the treatment of.

Embodiment 2：

A kind of Chinese medicine composition for treating pharyngo-laryngitis chronica, including 8 parts of puncturevine caltrop fruit, 6 parts of semen astragali complanati, 8 parts of smilax, 9 parts of paris polyphylla, 8 parts of radix paeoniae rubra, 6 parts of Radix Paeoniae Alba, 7 parts of Longstamen Onion Bulb, 7 parts of Snakegourd Fruit, 9 parts of Radix Rehmanniae, 7 parts of the root of straight ladybell, 6 parts of Radix Ophiopogonis, 7 parts of the root bark of white mulberry, 6 parts of root of Chinese trichosanthes, 8 parts of honeysuckle, 8 parts of Fructus Forsythiae, 7 parts of lophatherum gracile, 7 parts of campanulaceae, 6 parts of peppermint, 6 parts of Radix Glycyrrhizae, 6 parts of the stem of noble dendrobium, 7 parts of Chinese globeflower, 7 parts of the bletilla striata, 7 parts of asparagus fern, 7 parts of radix scrophulariae, 8 parts of rhizoma atractylodis, 3 parts of cordyceps sinensis, 5 parts of Rhizoma Atractylodis Macrocephalae.

It further illustrates, above-mentioned substance is after the dry fruit of puncturevine caltrop fruit is carried out ultramicro grinding, and it is 85% that mass concentration, which is added, Ethanol solution carry out refluxing extraction 5 times, each extraction time is 10 minutes, will be extracted every time after filtered filtrate merges, It is concentrated into 1.12 times of relative density, you can puncturevine caltrop fruit extract is obtained, it is main containing flavone compound 4.7%, saponins compound 57.25 μ g/g, alkaloid 55.75mg/g, protein 1.86mg/g.

The seed of semen astragali complanati is carried out after being crushed, 10 times of aqueous solutions progress water are added and carry, the temperature that water carries is 60 DEG C, extraction Time be 40 minutes, water using the ultrasonic wave that power is 100W carries out assisted extraction during carrying, and filtrate after water is carried carries out Filtering, obtained Semen Astragali Complanati extract master contain flavonoids 6.82mg/g, alkaloid 2.24mg/g, triterpene substance 1.92mg/ g。

The 75wt% ethanol solutions that 5 times of volumes are added after smilax is crushed extract 0.3 hour, filter to take filtrate, filter Slag repeats to extract, and merges 3 filtrate, you can obtains smilax extracting solution, the main 32.45mg/g of substance containing saponin, succinic acid 2.01mg/g, palmitic acid 1.54mg/g, Quercetin 1.32mg/g.

The fresh quench stem of paris polyphylla is blended using blender, 8 times of water is subsequently added into and carries out Soxhlet extraction 2 hours, is collected Filtrate, by filtrate according to flow velocity be 0.2m/s by the Filter column containing polyether sulfone after, collect extracting solution, you can obtain paris polyphylla and carry Take liquid, the main 29.34mg/g of substance containing saponin, Flavonoid substances 16.89mg/g.

10 times of 65wt% ethanol solutions are added after the rhizome of radix paeoniae rubra is crushed to extract at 60 DEG C 3 times, every time when extraction Between be 2 hours, the extracting solution extracted every time is subjected to ethyl alcohol recycling, concentration is dried to obtain red paeonia extract, main total containing Chinese herbaceous peony Glycosides 19.75mg/g, tannin class 9.05mg/g, flavonoids 6.97mg/g, volatile oil 4.25mg/g.

After moisture content≤2% is dried in the rhizome of Radix Paeoniae Alba, smash it through 100 mesh sieve, by remaining large particulate matter after Continued powder is broken to be sieved with 100 mesh sieve, and it is 1 according to volume ratio that will then filter powder with distilled water:In 10 ratio input refluxing reactor It carries out refluxing extraction 4 times, 2 hours every time, filtrate is obtained after filtering, filtrate is spray-dried, obtain radix paeoniae alba extraction, it is main Contain glycoside substance 25.63mg/g, Flavonoid substances 24.13mg/g, polysaccharide 110.21mg/g.

Longstamen Onion Bulb is added in diatomite and carries out mortar grinder into pureed, is subsequently added into 10 times of 75wt% ethanol solutions in 60 DEG C Under extract 3 times, filter, merging filtrate, 10 times of 30wt% acetums, which are added, in filtrate carries out overnight precipitation extractions, filtering Take filtrate, you can obtain longstamen onion bulb extract, main A containing macrostemonoside 5.94mg/g, macrostemonoside D 2.16mg/g, macrostemonoside E 1.64mg/g, macrostemonoside F 1.59mg/g.

By the kernel of Snakegourd Fruit, pericarp according to weight ratio 1:It is put into steam copper and is accumulated after 1 hybrid slicing, steam is under Portion is sprayed to top heats, and the temperature of steam is 230 DEG C, and the high temperature mixing steam of boiling is directly transported by rising appendix Into the cooling tube of decline, is collected with liquefied pot after being -2 DEG C cooling in temperature, obtain what condensation obtained after repressurization liquefaction In substance mainly 18.64mg/g containing flavonoid glycoside, triterpenoid saponin 28.67mg/g, protein 18.16%, organic acid 12.64%, sugar Class 18.24%, 8.99% chemical composition of alkaloid.

After Radix Rehmanniae is cooked, stirring is slurried, and water is added, is gradually added into 10 times of 50wt% acetums and stirs into a paste, It is subsequently added into water, the hydrolysis of 50wt% acetums mixing standing 15 hours, three layers of filtered through gauze is crossed, is incorporated as filtrate after filtering The activated carbon of filtrate total volume 0.2%, cleans, and Radix Rehmanniae extracting solution, wherein paste, water, 50wt% vinegar are can be obtained after filtering The volume ratio of acid solution is 1:3: 10；By detecting Radix Rehmanniae extracting solution master containing martynoside 9.1%, dihydro Catalpol glycosides 2.8%, peach leaf Coral glycosides 1.8%, acteoside 1.1%, Catalpol glycosides 1.7%.

The root of straight ladybell rhizome is added to 12 times of water to decoct 2 times, 40 minutes every time, filters merging filtrate, and be concentrated under reduced pressure while hot It is 1.02 times to relative density, is subsequently added into 75wt% ethanol solutions and is stirred, is to carry out precipitation and separation at -5 DEG C in temperature, Take supernatant, ethanol solution be recovered by filtration, be concentrated into relative density be 1.10 times after, be spray-dried, obtain the root of straight ladybell extraction Object, it is main to contain triterpenoid saponin 12.43% and starch 25.46% by detection.

It after cleaning Radix Ophiopogonis, is blended using blender, is subsequently added into 10 times of water and carries out refluxing extraction 3 times, 2 is small every time When, filtering filtrate will merge three times, after drying is concentrated into 1.01 times of original volume, 80 wt% ethanol solutions be added and are carried out at 0 DEG C Alcohol extracting 8 hours, centrifugation take precipitation spray drying to obtain Radix Ophiopogonis activity extract, main to contain Ophiopogonin A by detection 25.73mg/g, ophiopogonin B 34.02mg/g, ophiopogonone A 20.46mg/g.

It after the root skin of the root bark of white mulberry is dried, crushes, 80 wt% ethanol solutions is added and carry out refluxing extraction 5 times under room temperature, return Stream extraction 30 minutes every time, filters to take filtrate, medicinal extract is obtained after being concentrated into 1.02 times of original volume while hot, and medicinal extract is used Sevage Method removes deproteinized, takes supernatant, and cellulase is added, after absolute ethyl alcohol and stirring in supernatant, is precipitated 10 hours, then use from Scheming 2000r/min centrifugal treatings 5 minutes, are filtered under diminished pressure taking precipitate and obtain mulberry bark extract, contain flavones by detection is main Class compound 65.27mg/g；Wherein supernatant：Cellulase：The volume ratio of absolute ethyl alcohol is 3:1: 8.

Root of Chinese trichosanthes be added in 12 times of water and is decocted, decoction divides three phases, 60 DEG C of small fire to decoct 30 points Clock, 90 DEG C of moderate heat decoct 20 minutes, and 60 DEG C of small fire decocts 20 minutes, and filtrate is then obtained by filtration, be added absolute ethyl alcohol into Row alcohol extracting stay overnight, centrifuging and taking filtrate obtains root of Chinese trichosanthes extracting solution, by detection, mainly contain polysaccharose substance 32.36mg/g, Protein 17.26mg/g, saponin(e 11.26mg/g and amino acid 9.45mg/g active ingredients.

The flower of honeysuckle is put into high temperature and pressure sealer, with steam pressure to 10 kilograms of pressure, temperature in 180 degree, from Under the action of heart rotation, the effective ingredient in Chinese medicine is separated in solution by 4 hours steam pressures, after natural cooling, is utilized Solution is put into freeze drier by micro computer control technology to be freeze-dried, and Flos Lonicerae extract is obtained, main by detecting Contain terpene 58.12mg/g, flavonoids 63.54mg/g, organic acid 19.12mg/g, 40.27 μ g/g of ferro element, calcium constituent 34.59 μ g/g, 1.45 μ g/g of Zn-ef ficiency, 6.58 μ g/g of manganese element.

It is put into high temperature and pressure sealer after the fruit of Fructus Forsythiae is crushed, with steam pressure to 12 kilograms of pressure, temperature exists 200 °, under the action of centrifugal rotation, the effective ingredient in Chinese medicine is separated in solution by 4 hours steam pressures, waits for that nature is cold But after, solution is put into freeze drier using micro computer control technology and is freeze-dried, forsythia suspense extraction is obtained, passed through The main 20.1mg/g containing flavonoids of detection, forsythin 65.3mg/g, Forsythinol glycosides C 10.4mg/g, Forsythinol glycosides D 11.2mg/g.

7 times of water are added after lophatherum gracile is crushed, temperature is to be stirred 20 minutes at 60 DEG C, stands overnight, is then heated to 100 Start to stir after DEG C, constant temperature stirs 2 hours, and centrifugal filtration while hot takes filtrate, and filter residue is repeated the above steps 3 times, merging filtrate Henon bamboo leaf extract is can be obtained, it is main containing general flavone 1.82%, polysaccharide 16.37%, protein 14.12%, ferro element by retrieval 68.20 μ g/g, 39.68 μ g/g of calcium constituent, 1.57 μ g/g of Zn-ef ficiency, 6.84 μ g/g of manganese element.

The rhizome of campanulaceae is passed through into supercritical CO₂Extraction equipment is extracted, and extract liquor is obtained, and is subsequently added into 80 wt% second Alcoholic solution alcohol extracting 3 hours, centrifugal filtration take filtrate, are concentrated under reduced pressure up to no ethyl alcohol taste, are diluted with water to 0.8g/ crude drug ml, By macroporous resin column, washed resin is then eluted using 80 wt% ethanol solutions, eluent depressurize dense to clarifying It is dried under reduced pressure after contracting, obtains Radix Platycodonis extract, contain total saposins 10.57% by retrieval is main.

It is put into steam copper and accumulates after the rhizome of peppermint is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 200 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen It obtains condensing after degree is -2 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains levorotatory menthol in obtained substance 75.43%, isomenthone 2.68%, pulegone 1.85%, peppermint isoflavone aglycone 3.85mg/g, Rosmarinic acid 18.42mg/g.

It crushed 20 mesh sieve after the rhizome of Radix Glycyrrhizae is dried in the shade, weigh 1g, petroleum ether is added and flows back 2 hours and dries in the shade, then adds Enter 75wt% ethanol solution refluxing extractions, the temperature of extraction is 60 DEG C, the time of extraction is 40 minutes, and extracting solution is obtained by filtration and subtracts Pressure is concentrated to dryness, and obtains licorice, by detection, mainly contains 3.97 μ g/g of triterpenoid saponin and flavone compound 121.34μg/g。

It is put into steam copper and accumulates after the rhizome of the stem of noble dendrobium is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 200 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen It obtains condensing after degree is -3 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains dendrobium polysaccharide in obtained substance 134.26mg/g, dendrobine 28.46mg/g, amino acid 1 17.69mg/g, ketone compounds 36.48mg/g.

After Chinese globeflower removal of impurities, cleaning, 20 mesh sieve is crushed, the brine that water diluted concentration is 5mol/l is then used to carry out It impregnates 30 minutes, is then extracted 20 minutes under 50kpa pressure, to cooling under normal pressure after extraction, filter to take filtrate, be concentrated under reduced pressure Chinese globeflower extract is can be obtained again to relative density 1.05, by the main 49.35mg/g containing alkaloid of detection, total flavonoid chemical combination Object 100.24mg/g, vitexin 39.46mg/g and orientoside 35.46mg/g.

By the rhizome of the bletilla striata, after crushing sieves with 100 mesh sieve, 10 times of water are added, while it is 250,000 IU/ that suitable enzyme activity, which is added, The cellulase of g carries out refluxing extraction 5 times, merging filtrate, 65wt% ethanol solutions is added in filtrate, stirring is positioned over temperature It is to stand 18 hours under subzero 2 degree, takes supernatant to recycle ethanol solution, bletilla striata extract is obtained after low temperature drying, by detection It is main to contain bletilla polysaccharide 80.13%.

The rhizome of asparagus fern is added after 12 times of water and impregnates 30 minutes, then 60 DEG C of small fires decoctions 30 minutes, 80 DEG C of moderate heats It decocts 15 minutes, closes natural cooling after fire, then 100 DEG C of big fire decoct 20 minutes again, and filtrate is filtered to take after being repeated 3 times to be obtained To asparagus fern extracting solution, amino acid 258.43mg/g, polysaccharide 167.42mg/g and saponins compound are mainly contained by detection 16.91mg/g。

The rhizome of radix scrophulariae is smashed it through into 20 mesh sieve, the 65wt% ethanol solutions that 12 times of volumes are added extract 30 Minute, it uses power to carry out assisted extraction for the ultrasonic wave of 400W during extraction, filters to take filtrate, filter residue is repeated the above steps Alcohol extracting 3 times, merging filtrate can be obtained by drying in inlet temperature is 300 DEG C, outlet temperature is 80 DEG C spray dryer Radix Scrophulariae extract, it is main containing harpagide 76.42%, harpagoside 2.86%, radix scrophulariae kind glycosides 1.41% by detecting.

It is put into steam copper and accumulates after the rhizome of rhizoma atractylodis is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 200 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen After degree is -2 DEG C cooling in collection and liquefied pot, obtain condensing after repressurization liquefaction mainly main containing terpenoid in obtained substance Object 12.40mg/g, polysaccharide 56.48mg/g, glucosides 91.24mg/g, amino acid 6.85%, mineral element 1.81%.

After the rhizome crushing of cordyceps sinensis is sieved with 100 mesh sieve, 10 times of water are added, while it is 250,000 that suitable enzyme activity, which is added, The cellulase of IU/g carries out refluxing extraction 5 times, merging filtrate, 65wt% ethanol solutions is added in filtrate, stirring is positioned over temperature Degree is to stand 18 hours under subzero 2 degree, and supernatant is taken to recycle ethanol solution, and it is cordyceps sinensis that main active is obtained after low temperature drying Polysaccharide 40.61mg/g.

The rhizome of Rhizoma Atractylodis Macrocephalae is added after 12 times of water and impregnates 30 minutes, then 60 DEG C of small fires decoctions 30 minutes, 80 DEG C of moderate heats It decocts 15 minutes, closes natural cooling after fire, then 100 DEG C of big fire decoct 20 minutes again, and filtrate is filtered to take after being repeated 3 times to be obtained To Rhizoma Atractylodis Macrocephalae extracting solution, by detecting main main 38.12mg/g containing polysaccharide, amino acid 9.12mg/g, flavones 12.94mg/g.It prepares Method：Above-mentioned raw materials are condensed into medicinal extract, borneol and auxiliary material is added, pill is made.It is oral for patient, 3 times a day, every time 10mg, 14 as a treatment course.

Embodiment 3：

A kind of Chinese medicine composition for treating pharyngo-laryngitis chronica is prepared including following raw material in parts by weight：Puncturevine caltrop fruit 1.5 parts, 1 part of semen astragali complanati, 2 parts of smilax, 1 part of paris polyphylla, 2 parts of radix paeoniae rubra, 1.5 parts of Radix Paeoniae Alba, 2 parts of Longstamen Onion Bulb, 1.5 parts of Snakegourd Fruit, Radix Rehmanniae 2 Part, 2 parts of the root of straight ladybell, 1.5 parts of Radix Ophiopogonis, 1.5 parts of the root bark of white mulberry, 2 parts of root of Chinese trichosanthes, 2 parts of honeysuckle, 2 parts of Fructus Forsythiae, 2 parts of lophatherum gracile, 2 parts of campanulaceae, 1 part of peppermint, 1 part of Radix Glycyrrhizae, 1 part of the stem of noble dendrobium, 2 parts of Chinese globeflower, 1.5 parts of the bletilla striata, 1.5 parts of asparagus fern, 1.5 parts of radix scrophulariae, 1.5 parts of rhizoma atractylodis, winter 0.5 part of worm summer grass, 1.5 parts of Rhizoma Atractylodis Macrocephalae.

It further illustrates, above-mentioned substance is after the dry fruit of puncturevine caltrop fruit is carried out ultramicro grinding, and it is 78% that mass concentration, which is added, Ethanol solution carries out refluxing extraction 4 times, and each extraction time is 6 minutes, after filtered filtrate merging will be extracted every time, concentration To 1.11 times of relative density, you can puncturevine caltrop fruit extract is obtained, it is main containing flavone compound 5.1%, 59.17 μ of saponins compound G/g, alkaloid 61.04mg/g, protein 2.14mg/g.

The seed of semen astragali complanati is carried out after being crushed, 6 times of aqueous solutions progress water are added and carry, the temperature that water carries is 54 DEG C, extraction Time be 33 minutes, water using the ultrasonic wave that power is 100W carries out assisted extraction during carrying, and filtrate after water is carried carries out Filtering, obtained Semen Astragali Complanati extract master contain flavonoids 7.31mg/g, alkaloid 2.47mg/g, triterpene substance 2.31mg/ g。

The 75wt% ethanol solutions that 4 times of volumes are added after smilax is crushed extract 0.22 hour, filter to take filtrate, Filter residue repeats to extract, and merges 3 filtrate, you can obtains smilax extracting solution, the main 39.47mg/g of substance containing saponin, succinic acid 2.38mg/g, palmitic acid 2.04mg/g, Quercetin 1.57mg/g.

The fresh quench stem of paris polyphylla is blended using blender, 6 times of water is subsequently added into and carries out Soxhlet extraction 1.2 hours, is received Collect filtrate, by filtrate according to flow velocity be 0.12m/s by the Filter column containing polyether sulfone after, collect extracting solution, you can obtain flea Not extracting solution, the main 35.62mg/g of substance containing saponin, Flavonoid substances 21.04mg/g.

9 times of 65wt% ethanol solutions are added after the rhizome of radix paeoniae rubra is crushed to extract at 60 DEG C 3 times, every time when extraction Between be 1.2 hours, the extracting solution extracted every time is subjected to ethyl alcohol recycling, concentration is dried to obtain red paeonia extract, and master contains Chinese herbaceous peony Total glycosides 25.26mg/g, tannin class 10.68mg/g, flavonoids 8.36mg/g, volatile oil 6.04mg/g.

After moisture content≤4% is dried in the rhizome of Radix Paeoniae Alba, smash it through 100 mesh sieve, by remaining large particulate matter after Continued powder is broken to be sieved with 100 mesh sieve, and it is 1 according to volume ratio that will then filter powder with distilled water:In 10 ratio input refluxing reactor It carries out refluxing extraction 3 times, 1.1 hours every time, filtrate is obtained after filtering, filtrate is spray-dried, obtains radix paeoniae alba extraction, It is main to contain glycoside substance 29.21mg/g, Flavonoid substances 28.18mg/g, polysaccharide 124.30mg/g.

Longstamen Onion Bulb is added in diatomite and carries out mortar grinder into pureed, is subsequently added into 6 times of 75wt% ethanol solutions at 60 DEG C It extracts 3 times, filters, merging filtrate, 6 times of 30wt% acetums, which are added, in filtrate carries out overnight precipitation extraction, filters to take filter Liquid, you can obtain longstamen onion bulb extract, main A containing macrostemonoside 6.54mg/g, macrostemonoside D 3.20mg/g, macrostemonoside E 2.08mg/g, Macrostemonoside F 2.64mg/g.

By the kernel of Snakegourd Fruit, pericarp according to weight ratio 1:It is put into steam copper and is accumulated after 1 hybrid slicing, steam is under Portion is sprayed to top heats, and the temperature of steam is 180 DEG C, and the high temperature mixing steam of boiling is directly transported by rising appendix Into the cooling tube of decline, is collected with liquefied pot after being -3 DEG C cooling in temperature, obtain what condensation obtained after repressurization liquefaction Mainly 23.45mg/g containing flavonoid glycoside, triterpenoid saponin 31.05mg/g, protein 20.6%, organic acid 13.5%, carbohydrate in substance 21.30%, 9.78% chemical composition of alkaloid.

After Radix Rehmanniae is cooked, stirring is slurried, and water is added, is gradually added into 6 times of 50wt% acetums and stirs into a paste, It is subsequently added into water, the hydrolysis of 50wt% acetums mixing standing 15 hours, three layers of filtered through gauze is crossed, is incorporated as filtrate after filtering The activated carbon of filtrate total volume 0.12%, cleans, and Radix Rehmanniae extracting solution, wherein paste, water, 50wt% vinegar are can be obtained after filtering The volume ratio of acid solution is 1:2:9；By detecting Radix Rehmanniae extracting solution master containing martynoside 11.2%, dihydro Catalpol glycosides 3.1%, peach leaf coral Coral glycosides 2.8%, acteoside 1.6%, Catalpol glycosides 2.4%.

The root of straight ladybell rhizome is added to 11 times of water to decoct 2 times, 32 minutes every time, filters merging filtrate, and be concentrated under reduced pressure while hot It is 1.01 times to relative density, is subsequently added into 75wt% ethanol solutions and is stirred, is to carry out precipitation and separation at -5 DEG C in temperature, Take supernatant, ethanol solution be recovered by filtration, be concentrated into relative density be 1.04 times after, be spray-dried, obtain the root of straight ladybell extraction Object, it is main to contain triterpenoid saponin 14.6% and starch 30.5% by detection.

It after cleaning Radix Ophiopogonis, is blended using blender, is subsequently added into 7 times of water and carries out refluxing extraction 3 times, every time 1.2 Hour, filtering filtrate will merge three times, it is dry be concentrated into 1.01 times of original volume after, be added 73 wt% ethanol solutions at -1 DEG C into Row alcohol extracting 6 hours, centrifugation take precipitation spray drying to obtain Radix Ophiopogonis activity extract, main to contain Ophiopogonin A by detection 28.14mg/g, ophiopogonin B 39.54mg/g, ophiopogonone A 25.37mg/g.

It after the root skin of the root bark of white mulberry is dried, crushes, 74 wt% ethanol solutions is added and carry out refluxing extraction 4 times under room temperature, return Stream extraction 25 minutes every time, filters to take filtrate, medicinal extract is obtained after being concentrated into 1.02 times of original volume while hot, and medicinal extract is used Sevage Method removes deproteinized, takes supernatant, and cellulase is added, after absolute ethyl alcohol and stirring in supernatant, is precipitated 10 hours, then use from Scheming 2000r/min centrifugal treatings 4 minutes, are filtered under diminished pressure taking precipitate and obtain mulberry bark extract, contain flavones by detection is main Class compound 74.6mg/g；Wherein supernatant：Cellulase：The volume ratio of absolute ethyl alcohol is 3:1:5.

Root of Chinese trichosanthes be added in 11 times of water and is decocted, decoction divides three phases, 53 DEG C of small fire to decoct 25 minutes, 85 DEG C of moderate heat decocts 14 minutes, and 55 DEG C of small fire decocts 12 minutes, and filtrate is then obtained by filtration, and absolute ethyl alcohol is added and carries out alcohol It carries overnight, centrifuging and taking filtrate obtains root of Chinese trichosanthes extracting solution, by detection, mainly contains polysaccharose substance 35.61mg/g, albumen Matter 20.17mg/g, saponin(e 13.54mg/g and amino acid 1 1.37mg/g active ingredients.

The flower of honeysuckle is put into high temperature and pressure sealer, with steam pressure to 7 kilograms of pressure, temperature is being centrifuged at 130 degree Under the action of rotation, the effective ingredient in Chinese medicine is separated in solution by 3 hours steam pressures, after natural cooling, utilization is micro- Solution is put into freeze drier and is freeze-dried by computer control technology, obtains Flos Lonicerae extract, mainly contains by detection Have terpene 68.1mg/g, flavonoids 75.4mg/g, organic acid 24.7mg/g, 45.21 μ g/g of ferro element, 38.69 μ g/g of calcium constituent, 1.87 μ g/g of Zn-ef ficiency, 7.50 μ g/g of manganese element.

It is put into high temperature and pressure sealer after the fruit of Fructus Forsythiae is crushed, with steam pressure to 9 kilograms of pressure, temperature exists 185 °, under the action of centrifugal rotation, the effective ingredient in Chinese medicine is separated in solution by 2.2 hours steam pressures, waits for nature After cooling, solution is put into freeze drier using micro computer control technology and is freeze-dried, forsythia suspense extraction is obtained, passed through Cross the main 25.8mg/g containing flavonoids of detection, forsythin 71.4mg/g, Forsythinol glycosides C 12.4mg/g, Forsythinol glycosides D 12.3mg/ g。

6.2 times of water are added after lophatherum gracile is crushed, temperature is to be stirred 13 minutes at 53 DEG C, stands overnight, is then heated to Start to stir after 100 DEG C, constant temperature stirs 2 hours, and centrifugal filtration while hot takes filtrate, and filter residue is repeated the above steps 3 times, merges Filtrate can be obtained henon bamboo leaf extract, main containing general flavone 2.15%, polysaccharide 18.64%, protein 15.2%, iron member by retrieval 72.06 μ g/g of element, 48.68 μ g/g of calcium constituent, 2.64 μ g/g of Zn-ef ficiency, 8.38 μ g/g of manganese element.

The rhizome of campanulaceae is extracted by SCF-CO 2 equipment, extract liquor is obtained, is subsequently added into 75 wt% second Alcoholic solution alcohol extracting 2.2 hours, centrifugal filtration take filtrate, are concentrated under reduced pressure up to no ethyl alcohol taste, are diluted with water to 0.8g/ crude drugs Ml, by macroporous resin column, washed resin is then eluted using 75 wt% ethanol solutions, eluent is subtracted to clarifying It is dried under reduced pressure after pressure concentration, obtains Radix Platycodonis extract, contain total saposins 11.16% by retrieval is main.

It is put into steam copper and accumulates after the rhizome of peppermint is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 190 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen It obtains condensing after degree is -3.5 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains left-handed peppermint in obtained substance Alcohol 78.5%, isomenthone 3.07%, pulegone 2.07%, peppermint isoflavone aglycone 5.12mg/g, Rosmarinic acid 21.08mg/g.

It crushed 20 mesh sieve after the rhizome of Radix Glycyrrhizae is dried in the shade, weigh 1g, petroleum ether is added and flows back 2 hours and dries in the shade, then adds Enter 68wt% ethanol solution refluxing extractions, the temperature of extraction is 55 DEG C, the time of extraction is 33 minutes, and extracting solution is obtained by filtration and subtracts Pressure is concentrated to dryness, and obtains licorice, by detection, mainly contains 4.25 μ g/g of triterpenoid saponin and flavone compound 134.26μg/g。

It is put into steam copper and accumulates after the rhizome of the stem of noble dendrobium is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 185 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen It obtains condensing after degree is -4 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains dendrobium polysaccharide in obtained substance 134.26mg/g, dendrobine 529.34mg/g, amino acid 5120.12mg/g, ketone compounds 538.04mg/g.

After Chinese globeflower removal of impurities, cleaning, 20 mesh sieve is crushed, the brine that water diluted concentration is 5mol/l is then used to carry out It impregnates 23 minutes, is then extracted 16 minutes under 35kpa pressure, to cooling under normal pressure after extraction, filter to take filtrate, be concentrated under reduced pressure Chinese globeflower extract is can be obtained again to relative density 1.03, by the main 54.12mg/g containing alkaloid of detection, total flavonoid chemical combination Object 115.21mg/g, vitexin 41.95mg/g and orientoside 38.18mg/g.

By the rhizome of the bletilla striata, after crushing sieves with 100 mesh sieve, 7 times of water are added, while it is 250,000 IU/g that suitable enzyme activity, which is added, Cellulase carry out refluxing extraction 4 times, 58wt% ethanol solutions are added in filtrate by merging filtrate, and stirring is positioned over temperature and is 14 hours are stood under subzero 4 degree, takes supernatant to recycle ethanol solution, bletilla striata extract is obtained after low temperature drying, is led by detection Contain bletilla polysaccharide 82.35%.

The rhizome of asparagus fern is added after 11 times of water and impregnates 24 minutes, then 58 DEG C of small fires decoctions 25 minutes, 73 DEG C of moderate heats It decocts 12 minutes, closes natural cooling after fire, then 93 DEG C of big fire decoct 13 minutes again, and filtrate is filtered to take after being repeated 3 times to be obtained To asparagus fern extracting solution, amino acid 261.24mg/g, polysaccharide 194.68mg/g and saponins compound are mainly contained by detection 17.14mg/g

The rhizome of radix scrophulariae is smashed it through into 20 mesh sieve, the 57wt% ethanol solutions that 11 times of volumes are added extract 17 minutes, It uses power to carry out assisted extraction for the ultrasonic wave of 320W during extraction, filters to take filtrate, filter residue is repeated the above steps alcohol extracting 2.5 times, merging filtrate, by inlet temperature is 225 DEG C, outlet temperature is 75 DEG C spray dryer it is dry can be obtained it is profound Conopsea extraction, it is main containing harpagide 78.14%, harpagoside 3.14%, radix scrophulariae kind glycosides 1.65% by detecting.

It is put into steam copper and accumulates after the rhizome of rhizoma atractylodis is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 189 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen After degree is -3 DEG C cooling in collection and liquefied pot, obtain condensing after repressurization liquefaction mainly main containing terpenoid in obtained substance Object 13.15mg/g, polysaccharide 64.35mg/g, glucosides 97.24mg/g, amino acid 7.24%, mineral element 2.36%.

After the rhizome crushing of cordyceps sinensis is sieved with 100 mesh sieve, 6 times of water are added, while it is 250,000 that suitable enzyme activity, which is added, The cellulase of IU/g carries out refluxing extraction 4 times, merging filtrate, 62wt% ethanol solutions is added in filtrate, stirring is positioned over temperature Degree is to stand 15 hours under subzero 3 degree, and supernatant is taken to recycle ethanol solution, and it is cordyceps sinensis that main active is obtained after low temperature drying Polysaccharide 41.05mg/g

The rhizome of Rhizoma Atractylodis Macrocephalae is added after 11 times of water and impregnates 28 minutes, then 54 DEG C of small fires decoct 23 minutes, 78 DEG C of moderate heats decoctions 13 minutes, close natural cooling after fire, then 96 DEG C of big fire decoct 17 minutes again, filtered to take after being repeated 3 times filtrate can be obtained it is white Art extracting solution, by detecting main main 39.58mg/g containing polysaccharide, amino acid 1 0.24mg/g, flavones 14.58mg/g.Preparation side Method：Above-mentioned raw materials are merged into medicinal extract, borneol and auxiliary material is added, pill is made.It is oral for patient, 3 times a day, every time 10mL, 14 as a treatment course.

Embodiment 4：

A kind of Chinese medicine composition for treating pharyngo-laryngitis chronica is prepared including following raw material in parts by weight：Puncturevine caltrop fruit 5.5 parts, 4 parts of semen astragali complanati, 6 parts of smilax, 7 parts of paris polyphylla, 5 parts of radix paeoniae rubra, 4 parts of Radix Paeoniae Alba, 5 parts of Longstamen Onion Bulb, 5 parts of Snakegourd Fruit, 6 parts of Radix Rehmanniae, sand 4 parts of ginseng, 4 parts of Radix Ophiopogonis, 5 parts of the root bark of white mulberry, 5 parts of root of Chinese trichosanthes, 6 parts of honeysuckle, 5 parts of Fructus Forsythiae, 5 parts of lophatherum gracile, 5 parts of campanulaceae, 4 parts of peppermint, 3 parts of Radix Glycyrrhizae, 3 parts of the stem of noble dendrobium, 5 parts of Chinese globeflower, 3.5 parts of the bletilla striata, 4.5 parts of asparagus fern, 4 parts of radix scrophulariae, 4 parts of rhizoma atractylodis, 2 parts of cordyceps sinensis, in vain 3.5 parts of art.

Further illustrate that above-mentioned substance is after the dry fruit of puncturevine caltrop fruit is carried out ultramicro grinding, it is 78% that mass concentration, which is added, Ethanol solution carry out refluxing extraction 3 times, each extraction time is 7 minutes, will be extracted every time after filtered filtrate merges, dense It is reduced to 1.12 times of relative density, you can puncturevine caltrop fruit extract is obtained, it is main containing flavone compound 4.7%, saponins compound 56.01 μ g/g, alkaloid 54.20mg/g, protein 1.74mg/g.

The seed of semen astragali complanati is carried out after being crushed, 7 times of aqueous solutions progress water are added and carry, the temperature that water carries is 56 DEG C, extraction Time be 36 minutes, water using the ultrasonic wave that power is 100W carries out assisted extraction during carrying, and filtrate after water is carried carries out Filtering, obtained Semen Astragali Complanati extract master contain flavonoids 6.72mg/g, alkaloid 2.28mg/g, triterpene substance 1.86mg/ g。

The 75wt% ethanol solutions that 3 times of volumes are added after smilax is crushed extract 0.24 hour, filter to take filtrate, Filter residue repeats to extract, and merges 3 filtrate, you can obtains smilax extracting solution, the main 32.37mg/g of substance containing saponin, succinic acid 1.92mg/g, palmitic acid 1.42mg/g, Quercetin 1.41mg/g.

The fresh quench stem of paris polyphylla is blended using blender, 7 times of water is subsequently added into and carries out Soxhlet extraction 1.5 hours, is received Collect filtrate, by filtrate according to flow velocity be 0.14m/s by the Filter column containing polyether sulfone after, collect extracting solution, you can obtain flea Not extracting solution, the main 30.12mg/g of substance containing saponin, Flavonoid substances 17.52mg/g.

9 times of 65wt% ethanol solutions are added after the rhizome of radix paeoniae rubra is crushed to extract at 60 DEG C 3 times, every time when extraction Between be 1.4 hours, the extracting solution extracted every time is subjected to ethyl alcohol recycling, concentration is dried to obtain red paeonia extract, and master contains Chinese herbaceous peony Total glycosides 20.21mg/g, tannin class 9.12mg/g, flavonoids 7.34mg/g, volatile oil 4.12mg/g.

After moisture content≤2% is dried in the rhizome of Radix Paeoniae Alba, smash it through 100 mesh sieve, by remaining large particulate matter after Continued powder is broken to be sieved with 100 mesh sieve, and it is 1 according to volume ratio that will then filter powder with distilled water:In 10 ratio input refluxing reactor It carries out refluxing extraction 3 times, 1.4 hours every time, filtrate is obtained after filtering, filtrate is spray-dried, obtains radix paeoniae alba extraction, It is main to contain glycoside substance 24.76mg/g, Flavonoid substances 25.17mg/g, polysaccharide 111.02mg/g.

Longstamen Onion Bulb is added in diatomite and carries out mortar grinder into pureed, is subsequently added into 7 times of 75wt% ethanol solutions at 60 DEG C It extracts 3 times, filters, merging filtrate, 6 times of 30wt% acetums, which are added, in filtrate carries out overnight precipitation extraction, filters to take filter Liquid, you can obtain longstamen onion bulb extract, main A containing macrostemonoside 5.72mg/g, macrostemonoside D 1.83mg/g, macrostemonoside E 1.81mg/g, Macrostemonoside F 1.72mg/g.

By the kernel of Snakegourd Fruit, pericarp according to weight ratio 1:It is put into steam copper and is accumulated after 1 hybrid slicing, steam is under Portion is sprayed to top heats, and the temperature of steam is 200 DEG C, and the high temperature mixing steam of boiling is directly transported by rising appendix Into the cooling tube of decline, is collected with liquefied pot after being -3 DEG C cooling in temperature, obtain what condensation obtained after repressurization liquefaction In substance mainly 18.34mg/g containing flavonoid glycoside, triterpenoid saponin 29.24mg/g, protein 19.34%, organic acid 11.72%, sugar Class 18.69%, 9.04% chemical composition of alkaloid.

After Radix Rehmanniae is cooked, stirring is slurried, and water is added, is gradually added into 7 times of 50wt% acetums and stirs into a paste, It is subsequently added into water, the hydrolysis of 50wt% acetums mixing standing 15 hours, three layers of filtered through gauze is crossed, is incorporated as filtrate after filtering The activated carbon of filtrate total volume 0.15%, cleans, and Radix Rehmanniae extracting solution, wherein paste, water, 50wt% vinegar are can be obtained after filtering The volume ratio of acid solution is 1:2:9；By detecting Radix Rehmanniae extracting solution master containing martynoside 10.5%, dihydro Catalpol glycosides 2.9%, peach leaf coral Coral glycosides 1.8%, acteoside 1.2%, Catalpol glycosides 1.7%.

The root of straight ladybell rhizome is added to 12 times of water to decoct 1 time, 38 minutes every time, filters merging filtrate, and be concentrated under reduced pressure while hot It is 1.01 times to relative density, is subsequently added into 75wt% ethanol solutions and is stirred, is to carry out precipitation and separation at -5 DEG C in temperature, Take supernatant, ethanol solution be recovered by filtration, be concentrated into relative density be 1.05 times after, be spray-dried, obtain the root of straight ladybell extraction Object, it is main to contain triterpenoid saponin 12.30% and starch 27.21% by detection.

It after cleaning Radix Ophiopogonis, is blended using blender, is subsequently added into 7 times of water and carries out refluxing extraction 3 times, every time 1.7 Hour, filtering filtrate will merge three times, it is dry be concentrated into 1.01 times of original volume after, be added 77wt% ethanol solutions at -1 DEG C into Row alcohol extracting 7 hours, centrifugation take precipitation spray drying to obtain Radix Ophiopogonis activity extract, main to contain Ophiopogonin A by detection 25.37mg/g, ophiopogonin B 38.21mg/g, ophiopogonone A 20.14mg/g.

It after the root skin of the root bark of white mulberry is dried, crushes, 78 wt% ethanol solutions is added and carry out refluxing extraction 4 times under room temperature, return Stream extraction 25 minutes every time, filters to take filtrate, medicinal extract is obtained after being concentrated into 1.02 times of original volume while hot, and medicinal extract is used Sevage Method removes deproteinized, takes supernatant, and cellulase is added, after absolute ethyl alcohol and stirring in supernatant, is precipitated 10 hours, then use from Scheming 2000r/min centrifugal treatings 3.5 minutes, are filtered under diminished pressure taking precipitate and obtain mulberry bark extract, main containing Huang by detection Ketone compounds 67.25mg/g；Wherein supernatant：Cellulase：The volume ratio of absolute ethyl alcohol is 2:1:7,

Root of Chinese trichosanthes be added in 11 times of water and is decocted, decoction divides three phases, 54 DEG C of small fire to decoct 25 minutes, 82 DEG C Moderate heat decoct 16 minutes, 57 DEG C of small fire decocts 18 minutes, and filtrate is then obtained by filtration, and absolute ethyl alcohol is added and carries out alcohol extracting Night, centrifuging and taking filtrate obtain root of Chinese trichosanthes extracting solution, by detection, mainly contain polysaccharose substance 32.37mg/g, protein 19.37mg/g, saponin(e 11.20mg/g and amino acid 1 0.23mg/g active ingredients.

The flower of honeysuckle is put into high temperature and pressure sealer, with steam pressure to 8 kilograms of pressure, temperature is being centrifuged at 155 degree Under the action of rotation, the effective ingredient in Chinese medicine is separated in solution by 3 hours steam pressures, after natural cooling, utilization is micro- Solution is put into freeze drier and is freeze-dried by computer control technology, obtains Flos Lonicerae extract, mainly contains by detection There are terpene 59.57mg/g, flavonoids 67.34mg/g, organic acid 19.37mg/g, 40.25 μ g/g of ferro element, 34.37 μ of calcium constituent G/g, 1.52 μ g/g of Zn-ef ficiency, 6.72 μ g/g of manganese element.

It is put into high temperature and pressure sealer after the fruit of Fructus Forsythiae is crushed, with steam pressure to 10 kilograms of pressure, temperature exists 170 °, under the action of centrifugal rotation, the effective ingredient in Chinese medicine is separated in solution by 2.8 hours steam pressures, waits for nature After cooling, solution is put into freeze drier using micro computer control technology and is freeze-dried, forsythia suspense extraction is obtained, passed through Cross the main 21.34mg/g containing flavonoids of detection, forsythin 67.25mg/g, Forsythinol glycosides C 10.27mg/g, Forsythinol glycosides D 11.20mg/g。

6 times of water are added after lophatherum gracile is crushed, temperature is to be stirred 19 minutes at 54 DEG C, stands overnight, is then heated to 100 Start to stir after DEG C, constant temperature stirs 2 hours, and centrifugal filtration while hot takes filtrate, and filter residue is repeated the above steps 2 times, merging filtrate Henon bamboo leaf extract is can be obtained, it is main containing general flavone 1.83%, polysaccharide 17.21%, protein 14.2%, ferro element by retrieval 65.34 μ g/g, 39.67 μ g/g of calcium constituent, 1.57 μ g/g of Zn-ef ficiency, 7.21 μ g/g of manganese element.

The rhizome of campanulaceae is extracted by SCF-CO 2 equipment, extract liquor is obtained, is subsequently added into 78 wt% second Alcoholic solution alcohol extracting 2.4 hours, centrifugal filtration take filtrate, are concentrated under reduced pressure up to no ethyl alcohol taste, are diluted with water to 0.8g/ crude drugs Ml, by macroporous resin column, washed resin is then eluted using 77wt% ethanol solutions, eluent is depressurized to clarifying It is dried under reduced pressure after concentration, obtains Radix Platycodonis extract, contain total saposins 10.23% by retrieval is main.

It is put into steam copper and accumulates after the rhizome of peppermint is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 192 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen It obtains condensing after degree is -4 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains levorotatory menthol in obtained substance 75.14%, isomenthone 2.57%, pulegone 1.86%, peppermint isoflavone aglycone 4.12mg/g, Rosmarinic acid 18.32mg/g.

It crushed 20 mesh sieve after the rhizome of Radix Glycyrrhizae is dried in the shade, weigh 1g, petroleum ether is added and flows back 2 hours and dries in the shade, then adds Enter 68wt% ethanol solution refluxing extractions, the temperature of extraction is 52 DEG C, the time of extraction is 38 minutes, and extracting solution is obtained by filtration and subtracts Pressure is concentrated to dryness, and obtains licorice, by detection, mainly contains 4.07 μ g/g of triterpenoid saponin and flavone compound 124.31μg/g。

It is put into steam copper and accumulates after the rhizome of the stem of noble dendrobium is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 193 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen It obtains condensing after degree is -5 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains dendrobium polysaccharide in obtained substance 130.22mg/g, dendrobine 27.54mg/g, amino acid 1 14.20mg/g, ketone compounds 35.67mg/g.

After Chinese globeflower removal of impurities, cleaning, 20 mesh sieve is crushed, the brine that water diluted concentration is 4mol/l is then used to carry out It impregnates 26 minutes, is then extracted 19 minutes under 42kpa pressure, to cooling under normal pressure after extraction, filter to take filtrate, be concentrated under reduced pressure Chinese globeflower extract is can be obtained again to relative density 1.04, by the main 42.37mg/g containing alkaloid of detection, total flavonoid chemical combination Object 102.36mg/g, vitexin 35.34mg/g and orientoside 37.20mg/g.

By the rhizome of the bletilla striata, after crushing sieves with 100 mesh sieve, 4 times of water are added, while it is 250,000 IU/g that suitable enzyme activity, which is added, Cellulase carry out refluxing extraction 4 times, 60wt% ethanol solutions are added in filtrate by merging filtrate, and stirring is positioned over temperature and is 12 hours are stood under subzero 3 degree, takes supernatant to recycle ethanol solution, bletilla striata extract is obtained after low temperature drying, is led by detection Contain bletilla polysaccharide 80.23%.

The rhizome of asparagus fern is added after 10 times of water and impregnates 27 minutes, then 56 DEG C of small fires decoctions 23 minutes, 78 DEG C of moderate heats It decocts 14 minutes, closes natural cooling after fire, then 96 DEG C of big fire decoct 17 minutes again, and filtrate is filtered to take after being repeated 3 times to be obtained To asparagus fern extracting solution, amino acid 250.28mg/g, polysaccharide 163.87mg/g and saponins compound are mainly contained by detection 16.54mg/g。

The rhizome of radix scrophulariae is smashed it through into 20 mesh sieve, the 62wt% ethanol solutions that 11 times of volumes are added extract 18 Minute, it uses power to carry out assisted extraction for the ultrasonic wave of 365W during extraction, filters to take filtrate, filter residue is repeated the above steps Alcohol extracting 2 times, merging filtrate can be obtained by drying in inlet temperature is 255 DEG C, outlet temperature is 77 DEG C spray dryer Radix Scrophulariae extract, it is main containing harpagide 77.35%, harpagoside 2.86%, radix scrophulariae kind glycosides 1.48% by detecting.

It is put into steam copper and accumulates after the rhizome of rhizoma atractylodis is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 188 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen After degree is -2 DEG C cooling in collection and liquefied pot, obtain condensing after repressurization liquefaction mainly main containing terpene in obtained substance Close object 12.04mg/g, polysaccharide 57.35mg/g, glucosides 90.35mg/g, amino acid 7.21%, mineral element 1.54%.

After the rhizome crushing of cordyceps sinensis is sieved with 100 mesh sieve, 7 times of water are added, while it is 250,000 that suitable enzyme activity, which is added, The cellulase of IU/g carries out refluxing extraction 4 times, merging filtrate, 59wt% ethanol solutions is added in filtrate, stirring is positioned over temperature Degree is to stand 12 hours under subzero 4 degree, and supernatant is taken to recycle ethanol solution, and it is cordyceps sinensis that main active is obtained after low temperature drying Polysaccharide 39.37mg/g.

The rhizome of Rhizoma Atractylodis Macrocephalae is added after 10 times of water and impregnates 27 minutes, then 55 DEG C of small fires decoctions 24 minutes, 77 DEG C of moderate heats It decocts 13 minutes, closes natural cooling after fire, then 93 DEG C of big fire decoct 16 minutes again, and filtrate is filtered to take after being repeated 3 times to be obtained To Rhizoma Atractylodis Macrocephalae extracting solution, by detecting main main 36.34mg/g containing polysaccharide, amino acid 9.34mg/g, flavones 11.34mg/g.It will be upper It states raw material and is merged into medicinal extract, borneol and auxiliary material is added, tablet is made.It is oral for patient, 3 times a day, 3 tablets each time, be within 14th One course for the treatment of.

Embodiment 5：

A kind of Chinese medicine composition for treating pharyngo-laryngitis chronica is prepared including following raw material in parts by weight：Puncturevine caltrop fruit 2.5 parts, 2 parts of semen astragali complanati, 3 parts of smilax, 2 parts of paris polyphylla, 3 parts of radix paeoniae rubra, 2 parts of Radix Paeoniae Alba, 3 parts of Longstamen Onion Bulb, 2 parts of Snakegourd Fruit, 3 parts of Radix Rehmanniae, sand 3 parts of ginseng, 2 parts of Radix Ophiopogonis, 3 parts of the root bark of white mulberry, 3 parts of root of Chinese trichosanthes, 3.5 parts of honeysuckle, 3.5 parts of Fructus Forsythiae, 4 parts of lophatherum gracile, 3 parts of campanulaceae, peppermint 2 Part, 2 parts of Radix Glycyrrhizae, 2 parts of the stem of noble dendrobium, 3 parts of Chinese globeflower, 2.5 parts of the bletilla striata, 3 parts of asparagus fern, 3 parts of radix scrophulariae, 2.5 parts of rhizoma atractylodis, 1 part of cordyceps sinensis, 2.5 parts of Rhizoma Atractylodis Macrocephalae.

It further illustrates, above-mentioned substance is specifically that after the dry fruit of puncturevine caltrop fruit is carried out ultramicro grinding, mass concentration is added Refluxing extraction is carried out for 82% ethanol solution 4 times, and each extraction time is 9 minutes, will extract filtered filtrate every time and merge Afterwards, it is concentrated into 1.11 times of relative density, you can puncturevine caltrop fruit extract is obtained, it is main containing flavone compound 4.9%, saponins chemical combination 56.37 μ g/g of object, alkaloid 53.68mg/g, protein 1.86mg/g.

The seed of semen astragali complanati is carried out after being crushed, 8 times of aqueous solutions progress water are added and carry, the temperature that water carries is 58 DEG C, extraction Time be 39 minutes, water using the ultrasonic wave that power is 100W carries out assisted extraction during carrying, and filtrate after water is carried carries out Filtering, obtained Semen Astragali Complanati extract master contain flavonoids 6.59mg/g, alkaloid 2.27mg/g, triterpene substance 2.04mg/ g。

The 75wt% ethanol solutions that 4 times of volumes are added after smilax is crushed extract 0.24 hour, filter to take filtrate, Filter residue repeats to extract, and merges 3 filtrate, you can obtains smilax extracting solution, the main 33.24mg/g of substance containing saponin, succinic acid 2.12mg/g, palmitic acid 1.71mg/g, Quercetin 1.42mg/g.

The fresh quench stem of paris polyphylla is blended using blender, 7 times of water is subsequently added into and carries out Soxhlet extraction 1.8 hours, is received Collect filtrate, by filtrate according to flow velocity be 0.14m/s by the Filter column containing polyether sulfone after, collect extracting solution, you can obtain flea Not extracting solution, the main 31.34mg/g of substance containing saponin, Flavonoid substances 17.52mg/g.

9 times of 65wt% ethanol solutions are added after the rhizome of radix paeoniae rubra is crushed to extract at 60 DEG C 3 times, every time when extraction Between be 1.9 hours, the extracting solution extracted every time is subjected to ethyl alcohol recycling, concentration is dried to obtain red paeonia extract, and master contains Chinese herbaceous peony Total glycosides 20.34mg/g, tannin class 8.97mg/g, flavonoids 7.23mg/g, volatile oil 5.31mg/g.

After moisture content≤1% is dried in the rhizome of Radix Paeoniae Alba, smash it through 100 mesh sieve, by remaining large particulate matter after Continued powder is broken to be sieved with 100 mesh sieve, and it is 1 according to volume ratio that will then filter powder with distilled water:In 10 ratio input refluxing reactor It carries out refluxing extraction 3 times, 1.7 hours every time, filtrate is obtained after filtering, filtrate is spray-dried, obtains radix paeoniae alba extraction, It is main to contain glycoside substance 23.27mg/g, Flavonoid substances 25.34mg/g, polysaccharide 111.11mg/g.

Longstamen Onion Bulb is added in diatomite and carries out mortar grinder into pureed, is subsequently added into 9 times of 75wt% ethanol solutions at 60 DEG C It extracts 3 times, filters, merging filtrate, 9 times of 30wt% acetums, which are added, in filtrate carries out overnight precipitation extraction, filters to take filter Liquid, you can obtain longstamen onion bulb extract, main A containing macrostemonoside 5.86mg/g, macrostemonoside D 2.07mg/g, macrostemonoside E 1.83mg/g, Macrostemonoside F 1.84mg/g.

By the kernel of Snakegourd Fruit, pericarp according to weight ratio 1:It is put into steam copper and is accumulated after 1 hybrid slicing, steam is under Portion is sprayed to top heats, and the temperature of steam is 215 DEG C, and the high temperature mixing steam of boiling is directly transported by rising appendix Into the cooling tube of decline, is collected with liquefied pot after being -4 DEG C cooling in temperature, obtain what condensation obtained after repressurization liquefaction In substance mainly 17.58mg/g containing flavonoid glycoside, triterpenoid saponin 28.37mg/g, protein 19.35%, organic acid 11.14%, sugar Class 17.21%, 8.78% chemical composition of alkaloid.

After Radix Rehmanniae is cooked, stirring is slurried, and water is added, is gradually added into 9 times of 50wt% acetums and stirs into a paste, It is subsequently added into water, the hydrolysis of 50wt% acetums mixing standing 15 hours, three layers of filtered through gauze is crossed, is incorporated as filtrate after filtering The activated carbon of filtrate total volume 0.17%, cleans, and Radix Rehmanniae extracting solution, wherein paste, water, 50wt% vinegar are can be obtained after filtering The volume ratio of acid solution is 1:3:9；By detecting Radix Rehmanniae extracting solution master containing martynoside 9.35%, dihydro Catalpol glycosides 2.9%, peach leaf coral Coral glycosides 2.4%, acteoside 1.2%, Catalpol glycosides 1.7%.

The root of straight ladybell rhizome is added to 10 times of water to decoct 2 times, 38 minutes every time, filters merging filtrate, and be concentrated under reduced pressure while hot It is 1.01 times to relative density, is subsequently added into 75wt% ethanol solutions and is stirred, is to carry out precipitation and separation at -5 DEG C in temperature, Take supernatant, ethanol solution be recovered by filtration, be concentrated into relative density be 1.08 times after, be spray-dried, obtain the root of straight ladybell extraction Object, it is main to contain triterpenoid saponin 12.57% and starch 27.24% by detection.

It after cleaning Radix Ophiopogonis, is blended using blender, is subsequently added into 9 times of water and carries out refluxing extraction 3 times, every time 1.3 Hour, filtering filtrate will merge three times, it is dry be concentrated into 1.01 times of original volume after, be added 79wt% ethanol solutions at -1 DEG C into Row alcohol extracting 7 hours, centrifugation take precipitation spray drying to obtain Radix Ophiopogonis activity extract, main to contain Ophiopogonin A by detection 24.28mg/g, ophiopogonin B 35.55mg/g, ophiopogonone A 21.34mg/g.

It after the root skin of the root bark of white mulberry is dried, crushes, 77 wt% ethanol solutions is added and carry out refluxing extraction 4 times under room temperature, return Stream extraction 26 minutes every time, filters to take filtrate, medicinal extract is obtained after being concentrated into 1.01 times of original volume while hot, and medicinal extract is used Sevage Method removes deproteinized, takes supernatant, and cellulase is added, after absolute ethyl alcohol and stirring in supernatant, is precipitated 10 hours, then use from Scheming 2000r/min centrifugal treatings 4 minutes, are filtered under diminished pressure taking precipitate and obtain mulberry bark extract, contain flavones by detection is main Class compound 68.37mg/g；Wherein supernatant：Cellulase：The volume ratio of absolute ethyl alcohol is 2:1:7.

Root of Chinese trichosanthes be added in 12 times of water and is decocted, decoction divides three phases, 58 DEG C of small fire to decoct 27 minutes, 85 DEG C of moderate heat decocts 16 minutes, and 59 DEG C of small fire decocts 19 minutes, and filtrate is then obtained by filtration, and absolute ethyl alcohol is added and carries out alcohol It carries overnight, centrifuging and taking filtrate obtains root of Chinese trichosanthes extracting solution, by detection, mainly contains polysaccharose substance 34.37mg/g, albumen Matter 18.37mg/g, saponin(e 12.34mg/g and amino acid 9.37mg/g active ingredients.

The flower of honeysuckle is put into high temperature and pressure sealer, with steam pressure to 9 kilograms of pressure, temperature is being centrifuged at 175 degree Under the action of rotation, the effective ingredient in Chinese medicine is separated in solution by 2.8 hours steam pressures, after natural cooling, is utilized Solution is put into freeze drier by micro computer control technology to be freeze-dried, and Flos Lonicerae extract is obtained, main by detecting Contain terpene 57.69mg/g, flavonoids 68.34mg/g, organic acid 19.37mg/g, 40.35 μ g/g of ferro element, calcium constituent 34.08 μ g/g, 1.15 μ g/g of Zn-ef ficiency, 6.57 μ g/g of manganese element.

It is put into high temperature and pressure sealer after the fruit of Fructus Forsythiae is crushed, with steam pressure to 11 kilograms of pressure, temperature exists 195 °, under the action of centrifugal rotation, the effective ingredient in Chinese medicine is separated in solution by 3.5 hours steam pressures, waits for nature After cooling, solution is put into freeze drier using micro computer control technology and is freeze-dried, forsythia suspense extraction is obtained, passed through Cross the main 22.3mg/g containing flavonoids of detection, forsythin 68.7mg/g, Forsythinol glycosides C 10.3mg/g, Forsythinol glycosides D 11.4mg/ g。

6 times of water are added after lophatherum gracile is crushed, temperature is to be stirred 13 minutes at 54 DEG C, stands overnight, is then heated to 100 Start to stir after DEG C, constant temperature stirs 2 hours, and centrifugal filtration while hot takes filtrate, and filter residue is repeated the above steps 3 times, merging filtrate Henon bamboo leaf extract is can be obtained, it is main containing general flavone 1.82%, polysaccharide 16.37%, protein 14.25%, ferro element by retrieval 60.34 μ g/g, 40.57 μ g/g of calcium constituent, 1.85 μ g/g of Zn-ef ficiency, 7.34 μ g/g of manganese element.

The rhizome of campanulaceae is passed through into supercritical CO₂Extraction equipment is extracted, and extract liquor is obtained, and is subsequently added into 76wt% second Alcoholic solution alcohol extracting 2.5 hours, centrifugal filtration take filtrate, are concentrated under reduced pressure up to no ethyl alcohol taste, are diluted with water to 0.8g/ crude drugs Ml, by macroporous resin column, washed resin is then eluted using 77wt% ethanol solutions, eluent is depressurized to clarifying It is dried under reduced pressure after concentration, obtains Radix Platycodonis extract, contain total saposins 10.04% by retrieval is main.

It is put into steam copper and accumulates after the rhizome of peppermint is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 195 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen It obtains condensing after degree is -3 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains levorotatory menthol in obtained substance 75.24%, isomenthone 2.58%, pulegone 1.7.33%, peppermint isoflavone aglycone 3.76mg/g, Rosmarinic acid 18.37mg/g.

It crushed 20 mesh sieve after the rhizome of Radix Glycyrrhizae is dried in the shade, weigh 1g, petroleum ether is added and flows back 2 hours and dries in the shade, then adds Enter 72wt% ethanol solution refluxing extractions, the temperature of extraction is 57 DEG C, the time of extraction is 38 minutes, and extracting solution is obtained by filtration and subtracts Pressure is concentrated to dryness, and obtains licorice, by detection, mainly contains 3.88 μ g/g of triterpenoid saponin and flavone compound 121.24μg/g。

It is put into steam copper and accumulates after the rhizome of the stem of noble dendrobium is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 187 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen It obtains condensing after degree is -4.5 DEG C cooling in collection and liquefied pot, after repressurization liquefaction and mainly contains dendrobium polysaccharide in obtained substance 128.35mg/g, dendrobine 27.35mg/g, amino acid 1 18.24mg/g, ketone compounds 37.20mg/g.

By Chinese globeflower removal of impurities, clean after, crushed 20 mesh sieve, then use water diluted concentration be 3.5mol/l brine into Row impregnates 27 minutes, is then extracted 18 minutes under 42kpa pressure, to cooling under normal pressure after extraction, filters to take filtrate, decompression is dense It is reduced to relative density 1.03 and can be obtained Chinese globeflower extract again, by the main 41.21mg/g containing alkaloid of detection, total flavonoid Close object 100.47mg/g, vitexin 38.34mg/g and orientoside 35.34mg/g.

By the rhizome of the bletilla striata, after crushing sieves with 100 mesh sieve, 9 times of water are added, while it is 250,000 IU/g that suitable enzyme activity, which is added, Cellulase carry out refluxing extraction 3 times, 76wt% ethanol solutions are added in filtrate by merging filtrate, and stirring is positioned over temperature and is 17 hours are stood under subzero 4.5 degree, takes supernatant to recycle ethanol solution, bletilla striata extract is obtained after low temperature drying, by detection It is main to contain bletilla polysaccharide 80.27%.

The rhizome of asparagus fern is added after 11 times of water and impregnates 22 minutes, then 57 DEG C of small fires decoctions 26 minutes, 78 DEG C of moderate heats It decocts 14 minutes, closes natural cooling after fire, then 98 DEG C of big fire decoct 19 minutes again, and filtrate is filtered to take after being repeated 3 times to be obtained To asparagus fern extracting solution, amino acid 250.31mg/g, polysaccharide 175.31mg/g and saponins compound are mainly contained by detection 16.37mg/g。

The rhizome of radix scrophulariae is smashed it through into 20 mesh sieve, the 61wt% ethanol solutions that 11 times of volumes are added extract 17 Minute, it uses power to carry out assisted extraction for the ultrasonic wave of 355W during extraction, filters to take filtrate, filter residue is repeated the above steps Alcohol extracting 3 times, merging filtrate can be obtained by drying in inlet temperature is 290 DEG C, outlet temperature is 79 DEG C spray dryer Radix Scrophulariae extract, it is main containing harpagide 78.14%, harpagoside 2.83%, radix scrophulariae kind glycosides 1.54% by detecting.

It is put into steam copper and accumulates after the rhizome of rhizoma atractylodis is sliced, steam is sprayed from lower to upper part to be heated, steam Temperature be 186 DEG C, by the high temperature mixing steam of boiling by rising appendix be delivered directly in the cooling tube of decline, Yu Wen After degree is -3.5 DEG C cooling in collection and liquefied pot, obtain condensing after repressurization liquefaction mainly main containing terpene in obtained substance Close object 12.37mg/g, polysaccharide 58.37mg/g, glucosides 91.34mg/g, amino acid 6.57%, mineral element 1.76%.

After the rhizome crushing of cordyceps sinensis is sieved with 100 mesh sieve, 8 times of water are added, while it is 250,000 that suitable enzyme activity, which is added, The cellulase of IU/g carries out refluxing extraction 4 times, merging filtrate, 63wt% ethanol solutions is added in filtrate, stirring is positioned over temperature Degree is to stand 17 hours under subzero 3.5 degree, and supernatant is taken to recycle ethanol solution, and it is worm that main active is obtained after low temperature drying Grass polysaccharide 39..37mg/g.

The rhizome of Rhizoma Atractylodis Macrocephalae is added after 11 times of water and impregnates 29 minutes, then 58 DEG C of small fires decoctions 27 minutes, 78 DEG C of moderate heats It decocts 13 minutes, closes natural cooling after fire, then 97 DEG C of big fire decoct 13 minutes again, and filtrate is filtered to take after being repeated 3 times to be obtained To Rhizoma Atractylodis Macrocephalae extracting solution, by detecting main main 36.33mg/g containing polysaccharide, amino acid 9.34mg/g, flavones 12.37mg/g.It will be upper It states raw material and is merged into medicinal extract, borneol and auxiliary material is added, capsule is made.It is oral for patient, 3 times a day, 3 tablets each time, 14 days It is as a treatment course.

Verification experimental verification：

1. observe the extreme clearing heat and detoxicating, relieving sore throat and acesodyne of pharmaceutical composition, the effect to wet one's whistle of promoting the production of body fluid, for the medicine clinical test into Row is in the related Pharmacodynamics experiment of major function.

Decoction is made in 1.1 pharmaceutical composition pills.Content：Crude drug 4g/ml liquids, pH=4.20 are decocted using distilled water It boils.Buy healthy rat（200±20g）, mouse（20±5g）Guangxi University of Chinese Medicine's Experimental Animal Center provides, laboratory Temperature be 22-28 DEG C, humidity 50-70%.

1.1.1 the experiment of anti-inflammatory effect：

The effect always for the Mice Auricle inflammatory swelling that the extreme local application of pharmaceutical composition induces croton oil：Test mice Weight（20±5g）, each half of male and female.10 one group, 5 groups altogether, with containing 2% croton oil, 20% absolute ethyl alcohol, 5% distilled water, Two sides causes inflammation before and after the proinflammatory agent 0.05ml of 70% ether mixing is applied to the left ear of mouse, is given per hour before and after cause inflammation and causes inflammation survey ear Only, infatuated proinflammatory agent takes off after 4 hours at neck the pharmaceutical composition pill liquid 0.05ml/ of 3 various dose concentration of partial smearing Dead mouse.Cut two ears.With the rubber stopper hole making drill of a diameter of 8ml, the circle auricle at the same position of two ears is swept away.Assay balance claims Weight is calculated using two auricle weight difference of every mouse or so as inflammatory swelling.Compare group difference, the results are shown in Table 1：

1 pharmaceutical composition pill liquid local application of table causes croton oil the effect of mouse ear inflammatory swelling

Group	Size of animal/only	Dose concentration（Ml/ is only）	Mouse ear swelling degree（mg.X±SD）	Swelling inhibiting rate（%）
					Control group	10	Water -0.05	21.52±1.15	-
Low dose group	10	16%-0.05	14.54±2.67*	32.62
					Middle dose group	10	32%-0.05	12.34±1.65**	42.55
High dose group	10	64%-0.05	10.04±2.04**	53.21
					Compositus mentholi cream group	10	0.05	11.13±2.31**	47.98

Significance analysis：* P compared with the control group<0.05、**P<0.01.

The results show that the inflammatory swelling that pharmaceutical composition liquid local administration induces croton oil has obvious inhibiting effect.

1.1.2 the inhibiting effect for the Xiao Shu auricle inflammatory swellings that pharmaceutical composition liquid gastric infusion induces croton oil：

Test mice（20±5g）, each half of male and female.It 10 one group, 5 groups altogether, is administered to ig after being deprived of food but not water 12 hours, 1 Ear only being smeared with 2% croton oil proinflammatory agent 0.05ml/ after hour and causing inflammation, mouse, which is taken off neck, after 4 hours puts to death, and is claimed with assay balance Weight is calculated using two auricle weight difference of every mouse or so as inflammatory swelling.Compare group difference, the results are shown in Table 2：

2 pharmaceutical composition pill liquid ig administrations of table cause croton oil the effect of mouse ear inflammatory swelling

Group	Size of animal/only	Dose concentration（g/kg）	Mouse ear swelling degree（mg.X±SD）	Swelling inhibiting rate（%）
					Control group	10	Equivalent water	20.65±2.64	-
Low dose group	10	10	16.07±2.07*	22.2
					Middle dose group	10	20	13.43±3.02**	34.96
High dose group	10	40	10.01±3.15**	51.53
					Hydrocortisone group	10	25mg.ip	9.12±2.67**	55.84

Significance analysis：* P compared with the control group<0.05、**P<0.01.

The results show that ig administrations 10g/kg, 20 g/kg, 40 g/kg cause croton oil scorching Mice Auricle inflammatory swelling Play the role of apparent anti-inflammation detumescence I.

1.1.3 pharmaceutical composition liquid is locally applied to the granulomatous inhibiting effect of murine chronic

Test mice（20±5g）, each half of male and female.10 one group, 5 groups altogether, under the shallow fiber crops of ether, skin routinely disappears mouse Poison, sterile working.Abdomen skin 1cm is cut along median line, detaches subcutaneous tissue with vessel forceps, the skin at the groin of left and right respectively buries Enter to measure the filter paper 1 of antibiotic activity, skin suture.Started to be administered with the postoperative same day, local skin coating 0.05ml/ Only, three times a day, continuous 7 days, positive controls applied KANGZUO WUJI GAO to part.Animal, carefulness stripping granuloma are put to death after 7 days It is extracted together with filter paper, weighs after the drying 24 hours to constant weight of 60 DEG C of oven, subtract filter paper weight, as granuloma is net Weight, compares group difference, the results are shown in Table 3：

3 pharmaceutical composition pill liquid partial smearing of table is to the granulomatous inhibiting effect of murine chronic

Group	Size of animal/only	Dose concentration（Ml/, 3 times/day）	Granuloma weight（Mg/10gBW, X ± SD）	Swelling inhibiting rate（%）
					Control group	10	Equivalent water	11.26±2.16	-
Low dose group	10	16%-0.05	9.68±2.57*	14.03
					Middle dose group	10	32%-0.05	7.26±1.65**	35.52
High dose group	10	64%-0.05	5.79±2.04**	48.58
					KANGZUO WUJI GAO group	10	0.05	3.86±2.31**	65.72

Significance analysis：* P compared with the control group<0.05、**P<0.01.

The results show that caused by filter paper buries in the liquid local application that pharmaceutical composition dose concentration is 32%, 64% Chronic granuloma has apparent inhibiting effect.

1.1.4 pharmaceutical composition liquid bacteriostatic test

Culture medium：Chocolate agar, meat extract soup, serum broth, ordinary flat, blood plate.

Bacterium solution：Strain is a few days ago inoculated in ordinary flat by the strain that this laboratory reports an error, experiment（It is flat that second chain is inoculated in blood Plate）, 37 DEG C are incubated 18 hours and activate, and the microbionation activated is taken out when experiment in meat extract soup, after 37 DEG C are incubated 6 hours, is used It is spare that meat extract soup makes 100 times of diluted bacterium solutions, and second chain is inoculated in after 37 DEG C of serum broth is incubated 18 hours, is made pair of meat extract soup It is spare after diluting again.

Method：Various concentration liquid is added in chocolate agar, drug containing a concentration of 1 is respectively prepared:10、1:15、1:20、 1:40 chocolate agar plate, colleague prepare not drug containing chocolate tablet and use as a contrast.0.01ml bacterium solutions are taken to be inoculated with respectively In above-mentioned tablet, 37 DEG C be incubated 24 hours after observe result.It the results are shown in Table 4：

4 pharmaceutical composition liquid MIC measurement results of table

The results show that dilution 1:20 pharmaceutical composition liquid link coccus B-mode to A races, staphylococcus aureus have inhibition to make With dilution 1:10 liquid has inhibiting effect to Candida albicans.

1.2 the experiment of cough-suppressing phlegm-dispelling functions：

1.2.1 resolve phlegm effect of the pharmaceutical composition liquid to mouse：

Experiment mice（20±5g）, each half of male and female.10 one group, 5 groups altogether, after being deprived of food but not water 12 hours, ig administrations 30 After minute, 5% phenol red aqueous solution 0.1ml/10gBW is injected intraperitoneally, neck is taken off after 30 minutes and is put to death, fixed, separator tube, No. 7 flat Scalp acupuncture head is inserted into tracheal strips 0.3cm, and ligation is fixed, and 2.24mmoL/L sodium bicarbonate solutions 0.5ML is drawn with 1ml orchid stamen syringes Washing lotion is sucked out in light and handy lavation back and forth 3 times, injects in colorimetric cylinder, draws sodium bicarbonate solution 0.5ml, same method again Washing lotion is sucked out in lavation 3 times, then third time repeats.Washing lotion merging three times is placed in colorimetric cylinder, adds 1moL/L sodium bicarbonate solutions 0.1ML, with flushing liquor in alkalinity.With 721 type spectrophotometers, wavelength 546nm surveys OD.A standard curve is done with phenol red, is utilized Curve calculates phenol red content（μg/ml）, compared with the control group, the results are shown in Table 5：

5 pharmaceutical composition liquid of table is to the phenol red method resolve phlegm effect of mouse

Group	Size of animal/only	Dose concentration（G/kg, 3 times/day）	Phenol red discharge rate（μ g/ml, X ± SD）	Relative percentage（%）
					Control group	10	Equivalent water	1.26±0.35	100
Low dose group	10	10	2.45±0.67*	194
					Middle dose group	10	20	3.07±0.58**	244
High dose group	10	40	3.94±0.81**	313
					Ammonium chloride group	10	1	3.27±0.66**	260

Significance analysis：* P compared with the control group<0.05、**P<0.01.

As a result it proves：Pharmaceutical composition liquid is in 3 dosage of 10,20,40 g/kg, and one time gastric infusion has obviously Resolve phlegm effect.

1.2.2 influence of the pharmaceutical composition liquid to rat expectoration amount（Capillary glass-tube method）

Rat（200±20g）, each half of male and female.10 one group, 5 groups altogether, after being deprived of food but not water 12 hours, yellow Jackets 30mg/kg, ip are anaesthetized, fixed, separator tube, with sharp injection needle between thyroid gland cartilage lower edge hits exactly two cartilaginous rings Aperture is pricked, capillary glass tube one is inserted into（Internal diameter 0.8mm, long 4-5cm）.Capillary glass-tube is set just to contact tracheae bottom table , when being full of by sputum in capillary, one is replaced immediately, with capillary glass-tube sputum in face to draw the sputum at tracheae rear portion Resolve phlegm effect of the length as evaluation drug, after first 2 hours normal secretions amounts are administered in record, gastric infusion 4 hours.Compare to Medicine forward backward averaging secretory volume per hour, the results are shown in Table 6：

Phlegm-dispelling functions of the 6 pharmaceutical composition liquid of table to rat（Capillary glass-tube method）

Significance analysis：* P compared with the control group<0.05、**P<0.01.

As a result it proves：In 3 dosage of 10,20,40 g/kg of pharmaceutical composition liquid, one time gastric infusion has to rat Apparent phlegm-dispelling functions.

1.2.3 antitussive action of the pharmaceutical composition liquid to mouse（Concentrated ammonia liquor spray-on process）

Experiment mice（20±5g）, each half of male and female.10 one group, 5 groups altogether, after being deprived of food but not water 12 hours, gastric infusion 1 Hour is followed by being sprayed, constant-pressure atomization device：By glass sprayer, pressure gauge（Special mercury decompression meter）, vacuum pump, mouse glass Glass container connection composition.By concentrated ammonia liquor under the constant pressure of 18.7Kpa（12.5%）Spray into 15 in mouse glass container ", stop 15 " Cause mouse cough, number of coughing in record mouse cough incubation period and 3 minutes compares group difference, the results are shown in Table 7：

7 pharmaceutical composition liquid of table（ig）Mouse is to antitussive action

Group	Size of animal/only	Dose concentration（g/kg）	Cough latent period（s）	Cough number/3min
					Control group	10	Equivalent water	40.26±5.64	71.5±13.50
Low dose group	10	10	43.62±6.15	28.63±5.26*
					Middle dose group	10	20	68.31±9.37*	27.02±4.85*
High dose group	10	40	81.23±8.67**	24.84±7.64**
					Codeine phosphate group	10	30mg.ip	75.02±4.26*	12.07±8.25**

Significance analysis：* P compared with the control group<0.05、**P<0.01.

The results show that in two dosage groups of a concentration of 20,40 g/kg of test dose, concentrated ammonia liquor spraying is drawn in administration The mouse cough risen has antitussive action.

1.2.4 antitussive action of the pharmaceutical composition liquid to mouse（Cough method is drawn in citric acid spraying）

Cavy is used in experiment（200±20g）, each half of male and female.10 one group, altogether 5 groups.Gastric infusion 1 hour sprays into 17.5% Citric acid soln, constant pressure 53.4Kpa（400mmHg）, it sprays 90 seconds, animal glass container 3L, the cough in record cavy 2 minutes Number, compared with the control group, t is examined administration group between carrying out group, the results are shown in Table 8：

8 pharmaceutical composition liquid of table draws cavy citric acid the antitussive action of cough

Group	Size of animal/only	Dose concentration（g/kg）	Cough number/3min
				Control group	10	Equivalent water	35.50±10.20
Low dose group	10	10	25.15±4.62*
				Middle dose group	10	20	20.20±5.50**
High dose group	10	40	20.00±4.51**
				Codeine phosphate group	10	5mg.ip	18.56±3.55**

Significance analysis：* P compared with the control group<0.05、**P<0.01.

Experiments have shown that：Pharmaceutical composition liquid 20,40 g/kg two dosage groups in, administration to dense citric acid spraying draws The guinea pig cough risen has antitussive action.

1.3 pharmaceutical composition liquid DNFB induce hour delayed allergy（DTH）Influence：

ICR mouse are selected in experiment（20±5g）, each half of male and female.10 one group, altogether 6 groups.Ig is administered, once a day, continuous 7 It, sensitization：Mouse web portion 3 × 3cm of depilation, 1%DNFB solution 50UL is uniformly smeared, and is strengthened 1 time within second day.The production of DTH reactions Raw and measurement：The 5th day after sensitization, 1%DNFB solution 10UL is uniformly applied to mouse right ear（Two sides）It is attacked, control group It is same to apply ear but non-sensitization.After attack after 24 hours, mouse or so ear interior thickness, formic acid or so are measured with digimatic calipers Ear thickness difference（10^-3m）, as mice auricle swelling degree；Then cervical dislocation puts to death mouse, takes mouse thymus, spleen, thymus gland （Spleen）Index=thymus gland（Spleen）Weight（mg）/ weight（g）It indicates, observes the difference of each group；It the results are shown in Table 9：

Influence of the 9 pharmaceutical composition liquid of table to DNFB inducing mouses DTH

Group	Size of animal/only	Dose concentration（g/kg）	Auricle swelling degree（10^-3M, X ± SD）	Thymus index（Mg/g, X ± SD）	Index and spleen index（Mg/g, X ± SD）
						Control group	10	Equivalent water	0.02±0.01	1.68±0.42	4.86±1.09
DTH groups	10	25mg.ip	0.48±0.03	3.68±0.83	5.86±1.28
						Low dose group	10	10	0.30±0.02	3.11±0.56	5.01±1.31*
Middle dose group	10	20	0.24±0.02	2.06±0.72**	4.20±1.12**
						High dose group	10	40	0.20±0.03	2.11±0.81**	4.06±1.18**
Cetacort group	10	25mg.ip	0.23±0.02	2.08±0.56**	4.09±1.20**

Significance analysis：* P compared with the control group<0.05、**P<0.01.

As a result it proves：Pharmaceutical composition liquid 20,40 g/kg two dosage groups in, administration it is slow to DNFB inducing mouses Hair style allergy has inhibiting effect.

Analgesic test of the 1.4 pharmaceutical composition liquids to hot plate method in mice

Male mice is selected in experiment, and by mouse as in hot-plate instrument, temperature is 55 ± 0.5 DEG C, measures the pain threshold of mouse, surveys 2 It is secondary, be spaced 5 minutes, using mouse lick metapedes be used as observation index, such as mouse on hot plate 60S still without pain react, taking-up according to 60S is counted, and the qualified mouse of selection pre-selection is randomly divided into 5 groups, and every group 12, each group measures the pain threshold before administration, then fills Stomach is administered, and the volume of administration is 10ml/kg, 1 time/d, for three days on end, 60,90,120 points is measured respectively after the administration of third day Measure pain threshold after clock, the numerical value that each time point measures is respectively compared with distilled water control group, using t check analyses, as a result It is shown in Table 10.

10 pharmaceutical composition liquid of table does mouse spy the influence of sore threshold（S, X ± SD）

Significance analysis：* P compared with the control group<0.05、**P<0.01.

As a result it proves：Pharmaceutical composition liquid local application in basic, normal, high dose concentration can improve hot plate method in mice The threshold of pain of caused induced pain.

Influence research of the 1.5 pharmaceutical composition liquids to mouse tail electro photoluminescence pain reaction

Select experiment mice（20±5g）, each half of male and female.10 one group, 5 groups altogether, mouse is placed in fixator when experiment, Light is carried on the back.The alcohol of tail portion mass concentration 75% cleans skin degreasing, applies leading for last layer thin uniform layer again after alcohol volatilizes Electricity paste, with YSD-5 type pharmacology physiographs.Stimulation mode：Single.0.5 second time, wave wide 100ms, voltage 50V.It is adjusted before administration Voltage, selects continued stimulus 3 times, is spaced 5 minutes, whipping is caused to position the pain of the mouse, change to mouse after measuring the threshold of pain Tail portion partial smearing liquid 0.05ml/ only, surveys pain threshold after 15 minutes, the results are shown in Table 11 again.

11 pharmaceutical composition liquid office of table applies the inhibiting effect to mouse tail electro photoluminescence（V, X ± SD）

Significance analysis：* P compared with the control group<0.05、**P<0.01.

As a result it proves：Pharmaceutical composition liquid has to improve to stimulate in 16%, 32%, 64% dose concentration local application to be caused The pain threshold of mouse pain sensation reaction.

The clinical test of 2 treatment pharyngo-laryngitis chronicas

2.1 test method

2.1.1 inclusion criteria：Bottleneck throat pain, odynophagia, dry pain, the feeling of burning heat sensation, fever and aversion to cold are included in disease Example.

2.1.2 100 people of selector combination of syndromes example required standard person.

The pill that the pharyngo-laryngitis chronica Chinese medicine composition of the application is prepared into, treatment group is 3 times edible daily, 5 tablets each time, Total 10g, takes after postprandial half an hour, 14 days as a treatment course in the morning, afternoon and evening every time.

2.3 treatment standards：

Recovery from illness：Symptom all disappears, and throat restores as before；

Effectively：Symptom is substantially reduced, and pharyngitis, feeling of pain disappear substantially, with obvious effects；

Effect unobvious：Before and after treatment without significant change or deterioration.

2.4 treatment results

After one course for the treatment of of data, wherein that fully recovers has 65 people, effective 32 people, inapparent 3 people of effect, cure rate reaches 65%, Effective percentage reaches 32%, and total effective rate reaches 97%.

Model case：

She so-and-so, man, 38 years old, people from Liuzhou, pharyngeal dry pain 2 months or so, it was invalid after 2 weeks once to take antibiotic, using this The pill that the pharyngo-laryngitis chronica Chinese medicine composition of application is prepared into, after two courses for the treatment of, symptom is improved, symptom after three courses for the treatment of All disappear, recovery from illness.

It is deep certain, female, 42 years old, Guangxi Yulin people, pharyngeal pain swallowed pain 1 month, took the pharyngo-laryngitis chronica of the application The pill that Chinese medicine composition is prepared into, after a course for the treatment of, cardinal symptom disappears, and after two courses for the treatment of, symptom is substantially reduced, feeling of pain It is basic to disappear, it is with obvious effects.

Liu, man, 28 years old, Guangxi people from Wuzhou, throat had foreign body sensation, pharyngeal secretion to be not easy to bring up symptom and continue for 2 wheat harvesting periods, take the pill that the pharyngo-laryngitis chronica Chinese medicine composition of the application is prepared into, and after a course for the treatment of, cardinal symptom disappears, After two courses for the treatment of, symptom is substantially reduced, and feeling of pain disappears substantially, with obvious effects.

Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims

1. a kind of Chinese medicine composition for treating pharyngo-laryngitis chronica, it is characterised in that：Include puncturevine caltrop fruit 1-8 in parts by weight Part, 0.5-6 parts of semen astragali complanati, 1-8 parts of smilax, 0.5-9 parts of paris polyphylla, 1-8 parts of radix paeoniae rubra, 0.5-6 parts of Radix Paeoniae Alba, 1-7 parts of Longstamen Onion Bulb, Snakegourd Fruit 0.5-7 parts, 1-9 parts of Radix Rehmanniae, 1-7 parts of the root of straight ladybell, Radix Ophiopogonis 0.5-6 part, 0.5-7 parts of the root bark of white mulberry, 1-6 parts of root of Chinese trichosanthes, honeysuckle 0.5-8 Part, 0.5-8 parts of Fructus Forsythiae, 1-7 parts of lophatherum gracile, 0.5-7 parts of campanulaceae, 0.5-6 parts of peppermint, 0.5-6 parts of Radix Glycyrrhizae, 0.5-6 parts of the stem of noble dendrobium, gold Even 1-7 parts of flower, 0.5-7 parts of the bletilla striata, 1-7 parts of asparagus fern, 0.5-7 parts of radix scrophulariae, 0.5-8 parts of rhizoma atractylodis, 0.01-3 parts of cordyceps sinensis, Rhizoma Atractylodis Macrocephalae 1-5 parts.

2. Chinese medicine composition according to claim 1, it is characterised in that：Include prepared by following raw material in parts by weight It forms：1.5-5.5 parts of puncturevine caltrop fruit, 1-4 parts of semen astragali complanati, 2-6 parts of smilax, 1-7 parts of paris polyphylla, 2-5 parts of radix paeoniae rubra, 1.5-4 parts of Radix Paeoniae Alba, 2-5 parts of Longstamen Onion Bulb, 1.5-5 parts of Snakegourd Fruit, 2-6 parts of Radix Rehmanniae, 2-4 parts of the root of straight ladybell, Radix Ophiopogonis 1.5-4 part, 1.5-5 parts of the root bark of white mulberry, root of Chinese trichosanthes 2-5 Part, 2-6 parts of honeysuckle, 2-5 parts of Fructus Forsythiae, 2-5 parts of lophatherum gracile, 2-5 parts of campanulaceae, 1-4 parts of peppermint, 1-3 parts of Radix Glycyrrhizae, 1-3 parts of the stem of noble dendrobium, gold Even 2-5 parts of flower, 1.5-3.5 parts of the bletilla striata, 1.5-4.5 parts of asparagus fern, 1.5-4 parts of radix scrophulariae, 1.5-4 parts of rhizoma atractylodis, cordyceps sinensis 0.5-2 Part, 1.5-3.5 parts of Rhizoma Atractylodis Macrocephalae.

3. Chinese medicine composition according to claim 1, it is characterised in that：Include prepared by following raw material in parts by weight It forms：2.5 parts of puncturevine caltrop fruit, 2 parts of semen astragali complanati, 3 parts of smilax, 2 parts of paris polyphylla, 3 parts of radix paeoniae rubra, 2 parts of Radix Paeoniae Alba, 3 parts of Longstamen Onion Bulb, 2 parts of Snakegourd Fruit, 3 parts of Radix Rehmanniae, 3 parts of the root of straight ladybell, 2 parts of Radix Ophiopogonis, 3 parts of the root bark of white mulberry, 3 parts of root of Chinese trichosanthes, 3.5 parts of honeysuckle, 3.5 parts of Fructus Forsythiae, 4 parts of lophatherum gracile, tangerine 3 parts of stalk, 2 parts of peppermint, 2 parts of Radix Glycyrrhizae, 2 parts of the stem of noble dendrobium, 3 parts of Chinese globeflower, 2.5 parts of the bletilla striata, 3 parts of asparagus fern, 3 parts of radix scrophulariae, 2.5 parts of rhizoma atractylodis, 1 part of cordyceps sinensis, 2.5 parts of Rhizoma Atractylodis Macrocephalae.

4. a kind of Chinese drugs agentia of Chinese medicine composition that treating pharyngo-laryngitis chronica, it is characterised in that：It is obtained by claim 1 Chinese medicine composition be made plus common auxiliary material in pharmacy.

5. Chinese drugs agentia according to claim 4, it is characterised in that：The dosage form of the Chinese drugs agentia be oral solution, tablet, Capsule or pill.