CN108513760A - A method of promoting Germination of Soybean Seed under Submergence stress - Google Patents
A method of promoting Germination of Soybean Seed under Submergence stress Download PDFInfo
- Publication number
- CN108513760A CN108513760A CN201810353078.XA CN201810353078A CN108513760A CN 108513760 A CN108513760 A CN 108513760A CN 201810353078 A CN201810353078 A CN 201810353078A CN 108513760 A CN108513760 A CN 108513760A
- Authority
- CN
- China
- Prior art keywords
- seed
- water
- soya seeds
- soybean seed
- germination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of under Submergence stress promotes the method for Germination of Soybean Seed, specially choose the soya seeds normally without apparent pest and disease damage, it puts it into water and causes 48 hours, water is advisable with just flooding seed, and ensure that seed is maintained at liquid level or less in this stage, it is removed from water later, room temperature condition is placed in and does next time.The method of the present invention is simple to operation, at low cost, to seed safety.
Description
Technical field
The invention belongs to seed processing technology fields, and in particular to a kind of to promote Germination of Soybean Seed under Submergence stress
Method.
Background technology
At present in terms of the initiation of soya seeds, existing technology is exactly to be carried out to seed using different seed initiators
Cause.Such as using hormone, plant growth regulator and PEG substances soak seed and then improves seed in sprouting and its children
Seedling build up after to the resistance of biotic and abiotic stress, have reached the effect of volume increase.
Although seed or seedling can be enhanced in these researchs before to a certain extent for the anti-of environment stress
Property, but it is still insufficient for the present invention.First, above-mentioned initiation conditions need specific initiator, increase one
Fixed cost input increases the input of production cost in the agricultural production process of large area.For the application, seed exists
Cause in water, greatly reduces the input of production cost；Secondly most of initiators are exactly a kind of abiotic for seed
Stress, such as PEG, salt, the dosage used is different in different seeds, holds that bad will be caused to seed can not
The injury retrieved, and water be nature it is very common be also one of indispensable principal element in Seed Germination, the present invention
It is middle to carry out seed priming using water, reduce the damage to seed in elicitation procedure.
Invention content
The purpose of the present invention is to provide a kind of in such a way that water causes soya seeds, to improve seed in waterflooding situation
Under germination rate.
The present invention is realized especially by following technical scheme：
A method of promoting Germination of Soybean Seed under Submergence stress, specifically includes following steps：
1) size chosen after natural air drying is uniform, full grains, and disease-free soya seeds are spare；
2) soya seeds of selection are placed in clear water and are caused；
3) after the completion of causing, it is removed from water, room temperature condition is placed in and does next time.
Further, the initiation time of the soya seeds in water is 4-8h.
Further, the clear water is advisable with just flooding seed, and initiating stage ensure seed be maintained at liquid level with
Under.
Further, time dry standard will not reduce as time went on for its weight.
Beneficial effects of the present invention are：
The present invention provides a kind of raising soya seeds in the method for the germination rate in waterflooding, and this method is simply easy
Operation, greatly reduces the input of production cost, while to seed safety in elicitation procedure, avoids using compared with prior art
Initiator discomfort causes the damage to seed, to influence yield.
Description of the drawings
Fig. 1 is that normal soya seeds are sprouted in different waters；
Fig. 2 is that the germination rate in different waters counts from the seed without initiation for seed by initiation；
Fig. 3 is to cause the germination rate for improving seed under the conditions of water logging；
Fig. 4 is that water causes the utilization ratio improved in Soybean Seeds Germination to oxygen；
Fig. 5 is expression quantity of the seed with the relevant gene of anaerobic respiration in germination process；
Fig. 6 is the measurement of conductivity in germination fluid in Seed Germination.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
The present invention is in such a way that water causes seed, to improve seed in the germination rate of waterflooding, primary operational mistake
Journey is as follows：
The size chosen after normal selection natural air drying is uniform, and full grains, disease-free soya seeds are put it into
Cause 4-8 hours in water, water is advisable with just flooding seed, and ensures that seed is maintained at liquid level or less in this stage.Later
It is removed from water, room temperature condition is placed in and does next time (returning dry standard will not reduce as time went on for its weight).
Embodiment 1
It is sprouted in different water by the soya seeds caused by water and normally without the soya seeds of water initiation.
Concrete operations are chosen normally without the soya seeds for causing processing and each 20 of the soya seeds caused by water, by it
Be placed in the general 9cm culture dishes for having double-layer filter paper, add wherein different waters (water normally sprouted be per ware 20mL,
20mL, 30mL, 40mL, 50mL, 60mL are set in this experiment).Its germination rate is counted during seed imbibition.
As shown in Figure 1, normal soya seeds germination rate in the case of water logging is remarkably decreased.A-F is normal soybean
Seed sprouted in different water after phenotypic map.G is the final germination rate data of statistics.This experiment is in 9cm culture dishes
Carry out, it is uniform to choose size in each culture dish, the soya seeds without apparent pest and disease damage 20, after add wherein it is different
Water waits for statistical data and taking pictures after its sprouting.
As shown in Fig. 2, normal soya seeds after causing by water its significantly improved in water logging germination rate.A-
D is aged seed by causing the statistical data for handling and being sprouted in different water without processing is caused.This experiment uses 9cm
Culture dish chooses 20, the seed without apparent pest and disease damage of health per ware, adds different water, unite during its sprouting
Count germination rate.Wherein A is addition water 20mL；B is addition water 30mL；C is addition water 35mL；D is addition water 40mL.E is normal
New soya seeds by causing processing and without causing the data statistics sprouted in different waters of processing.
Experimental data shows that normal soya seeds germination rate in the case of waterflooding significantly declines, when water to 50-
The germination rate of seed extremely significantly reduces when 60mL, and final germination rate only has 40% or so (Fig. 1) under normal condition.By water
For the seed of initiation under the conditions of water logging, germination rate will be significantly higher than the seed (Fig. 2) without initiation.
Embodiment 2
It is sprouted in different water by the soya seeds caused by water and normally without the soya seeds of water initiation.
Concrete operations are chosen normally without the soya seeds for causing processing and each 20 of the soya seeds caused by water, by it
Be placed in the 9cm culture dishes for being covered with double-layer filter paper, add wherein different waters (water normally sprouted be per ware 20mL,
20mL, 30mL, 40mL, 50mL, 60mL are set in this experiment).Its germination rate is counted during seed imbibition, and most to it
Whole germination rate carries out phenotypic map and takes pictures.
As shown in figure 3, normal soya seeds after causing by water its in water logging germination rate and seed vitality
It significantly improves.A-D is phenotype photo of the aged seed after causing processing and being sprouted in different water without initiation processing.
Wherein A and C is phenotypic map of the normal seed after causing and being sprouted in 20mL water without initiation processing；B and D is warp
Cross the phenotypic map caused and to be sprouted in 35mL water by the soya seeds caused.E and F is respectively that the seed of different disposal exists
The data statistics of radicle length and radicle weight after being sprouted in different water.Pass through the seed that water causes as seen from the figure, in water logging
In the case of its germination rate be obviously improved, while root long and fresh weight significantly improve.This experiment uses 9cm culture dishes, per ware
20, the seed without apparent pest and disease damage for choosing health, adds different water, germination rate is counted during its sprouting.A-
D is phenotype photo of the aged seed after causing processing and being sprouted in different water without initiation processing.Wherein A and C are
Phenotypic map of the normal seed after causing and being sprouted in 20mL water without initiation processing；B and D is by causing and being
The phenotypic map that soya seeds by initiation are sprouted in 35mL water.E and F is respectively the seed of different disposal in different water
The data statistics of radicle length and radicle weight after sprouting.
As shown in figure 4, normal soya seeds after causing by water its after water logging and the case where isolation outside air
Lower germination rate significantly improves.A-D is by causing processing and completely cutting off the external world in different water without the seed for causing processing
Air sprout after phenotype photo.Wherein A and B is normal seed by causing and is handled in 20mL water without causing
Completely cut off the phenotypic map after being sprouted under extraneous air conditions；C and D be through initiation and without initiation soya seeds in 35mL
Completely cut off the phenotypic map sprouted under extraneous air conditions in water.E and F is respectively the seed of above-mentioned different disposal in different water
The data statistics of germination rate.E is to cause the starvation sprouting in 20mL water with undischarged seed, and F is to cause and do not cause
Seed in 35mL water starvation sprout.This experiment use 9cm culture dishes, per ware choose health without apparent pest and disease damage
20, seed, add different water, later sealed culture dish with sealed membrane, ensure and extraneous air exclusion.
As shown in figure 5, normal soya seeds after hair is handled by water after its obtained by anaerobic respiration approach
The ability of energy significantly enhances.Different time points is sampled during seed is sprouted, and detects anaerobic respiration respectively
Alcohol metabolism relevant key gene GmPDC2, GmADH1 and GmADH3 in the process.The above results show normal soya seeds
After water causes, the expression quantity of two key genes during anaerobic respiration significantly raises, and can obtain more
Energy maintain the progress of basic vital movement, and then improve germination rate.
As shown in fig. 6, the degree of injury of normal soya seeds its biomembrane after the hair processing after water is notable
Ground reduces, it may be possible to due to the repair ability enhancing of biomembrane during initiation.In figure by water cause with without
The seed that water causes is sprouted in different water, and conductance measurement is carried out in different germination processes.
Therefore, the seed caused by water not only can improve its germination rate in brine, but also can be certain
Enhance the vigor of seed in degree.Its radicle weight and radicle length all increase significantly (Fig. 3) after sprouting.And cause by water
Seed in the case of starvation, can significantly improve its germination efficiency.The sprouting of seed is one and needs oxygen
The process of heterotrophism needs to carry out oxygenolysis to the energy matter that Interior Seed stores, releases energy and provided for the sprouting of seed
Energy, in the case of isolation extraneous oxygen, it is first to strengthen Interior Seed that seed, which can still have stronger germination rate,
The ability of anaerobic respiration, and then energy (Fig. 4) is provided for the sprouting of seed, secondly, initiation processing improves seed and was sprouting
For the utilization ratio of oxygen in journey, the expression quantity (Fig. 5) of gene crucial in anaerobic respiration approach is increased；Finally, water draws
Hair handles the increment repair ability of biomembrane, reduces the extravasation of intracellular electrolyte, strengthens the vigor (figure of seed
6)。
Claims (4)
1. a kind of method promoting Germination of Soybean Seed under Submergence stress, which is characterized in that include the following steps：
1) size chosen after natural air drying is uniform, full grains, and disease-free soya seeds are spare；
2) soya seeds of selection are placed in clear water and are caused；
3) after the completion of causing, it is removed from water, room temperature condition is placed in and does next time.
2. a kind of method promoting Germination of Soybean Seed under Submergence stress according to claim 1, which is characterized in that institute
The initiation time of the soya seeds stated in water is 4-8h.
3. a kind of method promoting Germination of Soybean Seed under Submergence stress according to claim 1, which is characterized in that institute
The clear water stated is advisable with just flooding seed, and ensures that seed is maintained at liquid level or less in initiating stage.
4. a kind of method promoting Germination of Soybean Seed under Submergence stress according to claim 1, which is characterized in that institute
Time dry standard stated will not reduce as time went on for its weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810353078.XA CN108513760A (en) | 2018-04-19 | 2018-04-19 | A method of promoting Germination of Soybean Seed under Submergence stress |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810353078.XA CN108513760A (en) | 2018-04-19 | 2018-04-19 | A method of promoting Germination of Soybean Seed under Submergence stress |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108513760A true CN108513760A (en) | 2018-09-11 |
Family
ID=63429694
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810353078.XA Pending CN108513760A (en) | 2018-04-19 | 2018-04-19 | A method of promoting Germination of Soybean Seed under Submergence stress |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108513760A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112005653A (en) * | 2020-09-08 | 2020-12-01 | 哈尔滨理工大学 | Method for promoting mung bean seeds to germinate |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822140A (en) * | 2010-05-07 | 2010-09-08 | 中国农业科学院生物技术研究所 | Method for using nanometer materials for promoting sprouting and growth of soybean seeds under drought stress |
| WO2012046821A1 (en) * | 2010-10-04 | 2012-04-12 | Sumitomo Chemical Company, Limited | Method for reducing water stress in plants |
| CN106941810A (en) * | 2017-03-23 | 2017-07-14 | 四川农业大学 | A kind of method of the lower Germination of Soybean Seed of raising NaCl stress |
| CN107371530A (en) * | 2017-08-23 | 2017-11-24 | 四川农业大学 | A kind of method for improving anti-adversity ability of the soya seeds in duration of germination |
-
2018
- 2018-04-19 CN CN201810353078.XA patent/CN108513760A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822140A (en) * | 2010-05-07 | 2010-09-08 | 中国农业科学院生物技术研究所 | Method for using nanometer materials for promoting sprouting and growth of soybean seeds under drought stress |
| WO2012046821A1 (en) * | 2010-10-04 | 2012-04-12 | Sumitomo Chemical Company, Limited | Method for reducing water stress in plants |
| CN106941810A (en) * | 2017-03-23 | 2017-07-14 | 四川农业大学 | A kind of method of the lower Germination of Soybean Seed of raising NaCl stress |
| CN107371530A (en) * | 2017-08-23 | 2017-11-24 | 四川农业大学 | A kind of method for improving anti-adversity ability of the soya seeds in duration of germination |
Non-Patent Citations (5)
| Title |
|---|
| KAZEM GHASSEMI-GOLEZANI: "Seed hydro-priming, a simple way for improving mung-bean performance under water stress", 《INTERNATIONAL JOURNAL OF BIOSCIENCES》 * |
| RATIKANTA MAITI: "Seed priming: an efficient farmers" technology to improve seedling vigour, seedling establishment and crop productivity.", 《INTERNATIONAL JOURNAL OF BIO-RESOURCE AND STRESS MANAGEMENT》 * |
| 于红茹主编: "《现代育苗技术》", 28 February 2014 * |
| 李艳茹: "不同的引发方式对大豆种子活力的影响", 《安徽农业科学》 * |
| 杨小环: "种子引发对水分胁迫下大豆幼苗生理特性的影响", 《中国生态农业学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112005653A (en) * | 2020-09-08 | 2020-12-01 | 哈尔滨理工大学 | Method for promoting mung bean seeds to germinate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101817708B (en) | Plant source seed soaking agent and preparation method thereof | |
| CN107155569B (en) | High-quality and high-efficiency production method of pressed-sand sweet potato melons | |
| CN101898917B (en) | Pepper seed initiator | |
| CN105474959B (en) | Pinus massoniana tissue culture seedling twig short shoot cutting seedling method | |
| CN103999594A (en) | Method for economically and quickly breaking dormancy of sand rice seeds and accelerating sand rice seeds to germinate | |
| CN110150086A (en) | A kind of matrix and cultural method of the cultivation of salt-soda soil Tomato in Greenhouse | |
| CN108323270A (en) | The method for promoting tinosporae seed to sprout | |
| CN110100526A (en) | A kind of cultural method improving Thesium chinese survival rate | |
| CN105359856A (en) | Method and device for batch identification of wheat salt tolerance | |
| CN108513760A (en) | A method of promoting Germination of Soybean Seed under Submergence stress | |
| CN104604642A (en) | Energy-saving irrigation method for high yield of rice | |
| CN108393338B (en) | Method for repairing metal strontium polluted soil by reinforced ryegrass and application | |
| CN104381103A (en) | Identification method for salt tolerance of sweet sorghum | |
| CN105027746A (en) | Pelleting powder used for wrapping special-shaped and micro seeds | |
| CN104982271B (en) | A kind of method of Rice seeds treated and rice shoot to improve paddy rice resistance | |
| CN105660335B (en) | A kind of method and system for identifying moisture-proof barley variety | |
| CN104152443A (en) | Molecular marker linked with turnip clubroot resistant gene and method for obtaining molecular marker | |
| CN111990009B (en) | Method for promoting germination of Cotinus coggygria seeds in Sichuan | |
| CN109220058B (en) | Method for improving germination rate of wild buckwheat rhizome seeds | |
| CN109006298B (en) | Pinellia ternata planting method for inhibiting cadmium content enrichment | |
| CN103918378B (en) | Seed processing method for improving the capability of soybean for resisting saline-alkali stress | |
| CN105557443A (en) | Method for planting radix et caulis acanthopanacis senticosi in forest lands in northern China | |
| CN105340743A (en) | Method for rapidly detecting transgenic glyphosate-resistant cotton | |
| CN109618844A (en) | A kind of your round-grained rice 8 cultural methods of rice evening round-grained rice | |
| CN109320375B (en) | Organic fertilizer for promoting generation of iron film on rice root surface and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180911 |
|
| RJ01 | Rejection of invention patent application after publication |