CN108496779B - Preparation method of sphagnum propagule for peat land recovery - Google Patents
Preparation method of sphagnum propagule for peat land recovery Download PDFInfo
- Publication number
- CN108496779B CN108496779B CN201810313779.0A CN201810313779A CN108496779B CN 108496779 B CN108496779 B CN 108496779B CN 201810313779 A CN201810313779 A CN 201810313779A CN 108496779 B CN108496779 B CN 108496779B
- Authority
- CN
- China
- Prior art keywords
- sphagnum
- peat
- propagule
- propagules
- particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000003415 peat Substances 0.000 title claims abstract description 51
- 241000736285 Sphagnum Species 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000011084 recovery Methods 0.000 title description 14
- 239000000725 suspension Substances 0.000 claims abstract description 21
- 239000001963 growth medium Substances 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 14
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000002245 particle Substances 0.000 claims abstract description 9
- 239000000661 sodium alginate Substances 0.000 claims abstract description 9
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 9
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 9
- 238000009630 liquid culture Methods 0.000 claims abstract description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000005708 Sodium hypochlorite Substances 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 6
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000002791 soaking Methods 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 2
- 239000007836 KH2PO4 Substances 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- NGPGDYLVALNKEG-UHFFFAOYSA-N azanium;azane;2,3,4-trihydroxy-4-oxobutanoate Chemical compound [NH4+].[NH4+].[O-]C(=O)C(O)C(O)C([O-])=O NGPGDYLVALNKEG-UHFFFAOYSA-N 0.000 claims description 2
- 229910052564 epsomite Inorganic materials 0.000 claims description 2
- 230000006872 improvement Effects 0.000 claims description 2
- 229910052603 melanterite Inorganic materials 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- 238000010008 shearing Methods 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 1
- 238000009331 sowing Methods 0.000 claims 1
- 239000002775 capsule Substances 0.000 abstract description 14
- 241000196324 Embryophyta Species 0.000 abstract description 13
- 230000001568 sexual effect Effects 0.000 abstract description 8
- 239000007863 gel particle Substances 0.000 abstract description 6
- 238000005520 cutting process Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
- 230000000249 desinfective effect Effects 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 239000000499 gel Substances 0.000 description 7
- 239000008223 sterile water Substances 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 239000010902 straw Substances 0.000 description 4
- 239000012467 final product Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000005065 mining Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000007931 coated granule Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002686 phosphate fertilizer Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H11/00—Bryophytes, e.g. mosses, liverworts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Inorganic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to the technical field of ecological restoration, in particular to a preparation method of sphagnum propagules for restoring peat lands. Collecting sphagnum with capsule from field, cleaning, separating sphagnum capsule from plant, disinfecting capsule with alcohol, crushing to obtain spore suspension as sphagnum sexual propagule, cutting plant into segments, disinfecting with sodium hypochlorite to obtain sphagnum asexual propagule, stirring sphagnum propagule, liquid culture medium and sodium alginate together to obtain uniform viscous liquid, and adding calcium chloride solution to harden to obtain sphagnum propagule gel particles. And (3) scattering the particles into the peat land to be restored, and then introducing vegetation in the peat land restoration process after the particles are planted. The method realizes nutrition and protection of the sphagnum moss propagules by using the gel in a field environment, improves the survival rate of the sphagnum moss propagules, has strong adaptability, can be widely applied to various degraded peat lands, and has the advantages that the used gel is easy to degrade and has small influence on the peat lands.
Description
Technical Field
The invention relates to the technical field of ecological restoration, in particular to a preparation method of sphagnum propagules for restoring peat lands.
Background
The peat field is an important wetland type, can fix carbon dioxide in the atmosphere in the form of accumulated peat layers, and is an important carbon sink. However, factors such as climate change and agricultural production change the environment of the peat land, and the hydrological conditions and vegetation states of the peat land are changed, so that the ecological function of the peat land is degraded. Therefore, the recovery of the peat land becomes a research problem worldwide, and researchers develop a large amount of research on the recovery of the peat land in europe and north america and obtain abundant results.
The main damage of peat fields originates from artificial drainage and vegetation removal for the convenience of mechanical operations in peat mining and agricultural production; over a period of mining and drainage, the native vegetation on the peat land will assume a degraded state and, within the decades after cessation of destruction, the vegetation will remain in this degraded state and the original foreign species will multiply there, and the ecological function of the peat land will be altered as a result. Rochefort et al performed over twenty years of work in Canada, utilizing mechanized moss layer grafting (moss layer)r transfer technique, MLTT) for recovery. This is the most widely used technique available, which has six main steps: 1. preparation of the plot, removing all the unwanted vegetation on the plot, and leveling the terrain to make the water level more evenly distributed; 2. collecting propagules, namely collecting plants from natural peat lands for recovery; 3. introduction of propagules, recovery of the propagules by introduction in a ratio of 1:10, i.e. from 1m2The propagules collected from the collection field are sown to 10m2To be recovered; 4. protecting the propagules, namely covering the propagules with a hay straw layer to protect the propagules; 5. fertilizing, namely applying a phosphate fertilizer to the sphagnum moss; 6. and (5) rewetting, namely damming and plugging the primary drainage ditch and the secondary drainage ditch by using soil blocks, so that the water level is gradually lifted and recovered. The peat land to be recovered can grow a large amount of sphagnum after years, so that the peat land can be effectively recovered. However, this method requires the preparation of large quantities of peat moss propagule fragments and straw to ensure that they are viable enough, and the decomposition of straw in the field can release greenhouse gases and affect the temperature and humidity of the peat field to be recovered, which can have a series of negative effects on the ecological recovery of the peat field.
Sodium alginate is a common gel raw material, is non-toxic and widely applied to the food industry, and sodium alginate solution is matched with calcium chloride to form gel at normal temperature to wrap living plants, which is an advantage that agar and the like which are melted at high temperature and then formed do not have. Because of the characteristics, the sodium alginate gel is a common material of artificial seed coats, is widely applied to the rapid propagation technology of plants, can rapidly cultivate a large number of plant larvae and can be successfully transplanted outdoors. The technology can be combined in the peat land recovery process, an optimized scheme is provided for introducing and protecting the peat moss propagules, the survival rate of the peat moss propagules is improved, and simultaneously, hay straws which are used in large quantities in the original recovery scheme are replaced, and the negative influence on the peat land during the recovery process is reduced.
Disclosure of Invention
The invention aims to provide a gel particle wrapped with peat moss propagules for the vegetation recovery of peat lands, fully exerts the characteristics that the gel material can contain water and nutrition required by plant growth, protects the peat moss propagules in the field environment, and can provide a suitable material for the vegetation recovery engineering of degraded peat lands.
In order to achieve the above object, the present invention provides a method for preparing sphagnum moss propagule gel-coated granules, which comprises the following steps:
(1) sphagnum propagule preparation
Collecting sphagnum from the field in the growth period of capsule, classifying, cleaning and shearing to obtain sphagnum capsule and fresh plant fragments which can be used as sphagnum propagation materials, and disinfecting different types of propagation bodies by soaking with 75% alcohol or 1% sodium hypochlorite for a certain time for later use.
Sterilizing sphagnum sporophore in 75% alcohol for 3min, washing with sterile water, and crushing in proper amount of liquid culture medium to prepare spore suspension.
Cutting fresh sphagnum plant into 1-2cm long segments, soaking and sterilizing with 1% sodium hypochlorite solution for 1min, or culturing sphagnum spore suspension on solid culture medium to germinate and grow gametophyte plant to obtain sphagnum vegetative propagule.
(2) Preparation of culture medium
The culture medium used in the invention is an improved formula of Knops culture medium:
the Knops basic culture medium comprises the following components in percentage by weight: ca (NO)3)2·4H2O 1g/L，FeSO4·7H2O 13.5mg/L，MgSO4·7H2O 0.25g/L，KCl 0.25g/L，KH2PO40.25g/L。
The improvement method is that 5g/L of sucrose, 1g/L of ammonium tartrate and 36.7mg/L of EDTA are added, and water is added to dilute the mixture to half concentration, and the pH value is adjusted to be between 5.5 and 6.
When in use, the liquid culture medium can be directly used or a solid culture medium can be prepared by adding 2% (w/v) agar according to the requirement.
(3) Putting the sphagnum moss propagule into a liquid culture medium to prepare suspension, adding 2-5% (w/v) sodium alginate into the suspension, stirring to obtain uniform viscous liquid, adding the mixed suspension into 0.1M calcium chloride solution by using a measuring spoon, and hardening for 30-60min to obtain the sphagnum moss propagule gel particles.
The diameter of the particles is 1cm-3 cm.
(4) The particles are scattered to an area needing to be restored at the density of 500-2000 particles per square meter for planting, and then the vegetation in the wetland restoration can be reintroduced.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention realizes the nutrition and protection of the sphagnum propagule by using the gel in the field environment and improves the survival rate of the sphagnum propagule.
2. The invention has strong adaptability and can be widely applied to various degraded peat lands under different conditions.
3. The gel used in the present invention is easily degradable and has less impact on the ecological environment than hay which is deposited in the recovery zone in large quantities after use and is waiting to be decomposed.
Detailed Description
Example 1:
collecting sphagnum peat moss in the field, classifying, cleaning, collecting capsules, soaking and sterilizing the capsules in 75% alcohol for 3min, rinsing with sterile water, and crushing the capsules in a culture medium according to the proportion of adding 5mL of liquid culture medium into each capsule to prepare spore suspension, thus obtaining sphagnum peat moss sexual propagule suspension. Adding 2% (w/v) sodium alginate solution into the suspension, stirring to obtain uniform viscous liquid, and dripping the mixed suspension into 0.1M calcium chloride solution to harden for 30min to obtain the final product. The prepared sphagnum sexual propagule gel particles are scattered into a peat land to be restored according to the density of 500 sphagnum sexual propagules per square meter, and the vegetation reintroduction process required in the peat land restoration can be realized after the peat land is planted and grown.
Example 2:
collecting sphagnum in the field, classifying, cleaning, cutting the fresh plant into 2cm long segments, soaking the segments in 1% sodium hypochlorite solution for 1min, rinsing with sterile water, and adding into liquid culture medium to obtain suspension. Adding 5% (w/v) sodium alginate solution into the suspension, stirring to obtain uniform viscous liquid, and dripping the mixed suspension into 0.1M calcium chloride solution to harden for 60min to obtain the final product. The prepared sphagnum sexual propagule gel particles are scattered into a peat land to be restored according to the density of 2000 sphagnum sexual propagules per square meter, and the vegetation reintroduction process required in the peat land restoration can be realized after the peat land is planted and grown.
Example 3:
collecting sphagnum in the field, classifying, cleaning, collecting capsules, cutting fresh plants into 2 cm-long segments, soaking and sterilizing the capsules in 75% alcohol for 3min, rinsing the capsules with sterile water, crushing the capsules in a culture medium according to the proportion of adding 5mL of liquid culture medium into each capsule, preparing spore suspension, soaking and sterilizing the plant segments in 1% sodium hypochlorite solution for 1min, rinsing the plant segments with sterile water, and adding the rinsed plant segments into the spore suspension to obtain the sphagnum comprehensive propagule suspension. Adding 3% (w/v) sodium alginate solution into the suspension, stirring to obtain uniform viscous liquid, and dripping the mixed suspension into 0.1M calcium chloride solution to harden for 45min to obtain the final product. The prepared sphagnum sexual propagule gel particles are scattered into a peat land to be restored according to the density of 1000 sphagnum sexual propagules per square meter, and the vegetation reintroduction process required in the peat land restoration can be realized after the peat land is planted and grown.
Claims (3)
1. A preparation method of peat moss propagules for restoring peat lands is characterized by comprising the following steps:
(1) sphagnum propagule preparation
Collecting peat in the field, classifying, cleaning, shearing to obtain material for peat moss propagation as peat moss propagation body, and soaking different types of propagation bodies in alcohol or sodium hypochlorite solution for a certain time to sterilize for later use;
(2) selection of the culture Medium
The medium used in the present invention is KnThe improved formula of the ops culture medium comprises the following components in parts by weight: ca (NO)3)2·4H2O 1g/L，FeSO4·7H2O 13.5mgL/L，MgSO4·7H2O 0.25g/L，KCl 0.25g/L，KH2PO40.25 g/L; the improvement method comprises the steps of adding 5g/L of sucrose, 1g/L of ammonium tartrate and 36.7mg/L of EDTA, adding water to dilute to half of the concentration, and adjusting the pH value to be between 5.5 and 6;
(3) placing the sphagnum moss propagules in a liquid culture medium to prepare a suspension, adding sodium alginate with the mass volume fraction of 2-5% (w/v) into the suspension, stirring to obtain a uniform viscous liquid, adding the mixed suspension into a 0.1M calcium chloride solution by using a measuring spoon, and hardening for 30-60min to complete the preparation of the sphagnum moss propagules gel coated particles;
(4) the particles are scattered to an area needing to be restored according to a certain density to wait for planting, and then the vegetation in the wetland restoration can be reintroduced.
2. The method of claim 1, wherein the particles have a diameter of 1 to 3 cm.
3. The method as set forth in claim 1, characterized in that the peat land restoration sowing method is to sow the above particles at a density of 500-2000 per square meter to an area needing restoration and wait for planting to realize the reintroduction of vegetation in wetland restoration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810313779.0A CN108496779B (en) | 2018-04-10 | 2018-04-10 | Preparation method of sphagnum propagule for peat land recovery |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810313779.0A CN108496779B (en) | 2018-04-10 | 2018-04-10 | Preparation method of sphagnum propagule for peat land recovery |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108496779A CN108496779A (en) | 2018-09-07 |
| CN108496779B true CN108496779B (en) | 2020-06-02 |
Family
ID=63381164
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810313779.0A Expired - Fee Related CN108496779B (en) | 2018-04-10 | 2018-04-10 | Preparation method of sphagnum propagule for peat land recovery |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108496779B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2601311B (en) * | 2020-11-24 | 2022-11-23 | Micropropagation Services E M Ltd | Methods of in vitro culturing sphagnum |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609443A (en) * | 2013-11-15 | 2014-03-05 | 华东师范大学 | Method for obtaining and cultivating sphagnum protonema |
| CN103875527A (en) * | 2013-12-13 | 2014-06-25 | 岭南园林股份有限公司 | Method for preparing artificial seeds of submerged plant, vallisneria natans |
| CN104798498A (en) * | 2015-04-29 | 2015-07-29 | 广西大学 | Preparation method of cunninghamia lanceolata synthetic seed |
| CN106342448A (en) * | 2016-08-24 | 2017-01-25 | 徐金蝶 | Vegetable seed coating process |
-
2018
- 2018-04-10 CN CN201810313779.0A patent/CN108496779B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609443A (en) * | 2013-11-15 | 2014-03-05 | 华东师范大学 | Method for obtaining and cultivating sphagnum protonema |
| CN103875527A (en) * | 2013-12-13 | 2014-06-25 | 岭南园林股份有限公司 | Method for preparing artificial seeds of submerged plant, vallisneria natans |
| CN104798498A (en) * | 2015-04-29 | 2015-07-29 | 广西大学 | Preparation method of cunninghamia lanceolata synthetic seed |
| CN106342448A (en) * | 2016-08-24 | 2017-01-25 | 徐金蝶 | Vegetable seed coating process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108496779A (en) | 2018-09-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107517727B (en) | Plateau vegetation restoration composition and plateau area vegetation restoration method | |
| CN105170623B (en) | A kind of method of charcoal and plant combined restoration of soil polluted by heavy metal | |
| CN110089337B (en) | Method for collecting and raising seedlings of native mangrove plant elegans plum seeds | |
| CN102845253A (en) | Special base stock bag for oil-tea tree seedling cultivation and preparation method thereof | |
| CN104996138A (en) | Method for shortening seedling raising time limit of uncaria rhynchophylla seeds | |
| KR20180079227A (en) | Method for purifying heavy metal contaminated soil | |
| CN108738960A (en) | A kind of implantation methods of citrus | |
| Setiyo et al. | Improving physical and chemical soil characteristic on potatoes (Solanum tuberosum L.) cultivation by implementation of Leisa system | |
| CN101595831B (en) | Seedlings culturing method of rare or endangered species-acer yangbiense seedlings and cultivating method thereof | |
| CN105145033B (en) | A kind of cultural method of black fruit fructus lycii | |
| CN104429553A (en) | Production method for raising blueberry seedlings by cutting through containers | |
| CN103947442A (en) | Two-step seedling culture method of graft container seedlings of tea-oil tree | |
| CN113249224A (en) | Portable arbuscular mycorrhizal fungus application method | |
| CN105594500A (en) | Palm container seedling culture method | |
| CN100333634C (en) | Method for breeding bulb of east lily in test tube | |
| CN104770177B (en) | A kind of Rosa roxburghii isolated shoot method for culturing seedlings and the application in Rocky Desertification Control | |
| CN108496779B (en) | Preparation method of sphagnum propagule for peat land recovery | |
| CN105145257A (en) | High-efficiency paraphlomis javanica var.coronata forest building method | |
| CN211185232U (en) | Production facility of tea-oil camellia matrix of growing seedlings | |
| CN106954443A (en) | A kind of new rhizoma Gastrodiae implantation methods | |
| CN109169107A (en) | A kind of novel Rhizoma Gastrodiae implantation methods | |
| CN108541555B (en) | Transplanting nutrient medium and cultivation management method for sedum alfredii tissue culture seedlings | |
| CN110574660A (en) | production equipment and preparation method of camellia oleifera seedling raising matrix | |
| CN110558128A (en) | Industrial root seedling breeding method for hybrid paper mulberry | |
| CN113079966B (en) | Composite porous substrate for cistanche deserticola planting and preparation method and inoculation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Bu Zhaojun Inventor after: Liu Yu Inventor after: Wang Zhongqiang Inventor before: Liu Yu Inventor before: Bu Zhaojun Inventor before: Wang Zhongqiang |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200602 |