CN108496083A - 用于检测包含组蛋白修饰和变体的核小体的方法 - Google Patents

用于检测包含组蛋白修饰和变体的核小体的方法 Download PDF

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Publication number
CN108496083A
CN108496083A CN201680074822.1A CN201680074822A CN108496083A CN 108496083 A CN108496083 A CN 108496083A CN 201680074822 A CN201680074822 A CN 201680074822A CN 108496083 A CN108496083 A CN 108496083A
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CN
China
Prior art keywords
histone
variant
nucleosome
modification
isotype
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Pending
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CN201680074822.1A
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English (en)
Chinese (zh)
Inventor
J.V.米卡莱夫
M.E.埃克莱斯顿
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Belgium Wills LLC
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Belgium Wills LLC
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Application filed by Belgium Wills LLC filed Critical Belgium Wills LLC
Publication of CN108496083A publication Critical patent/CN108496083A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201680074822.1A 2015-10-21 2016-10-21 用于检测包含组蛋白修饰和变体的核小体的方法 Pending CN108496083A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1518674.5A GB201518674D0 (en) 2015-10-21 2015-10-21 Method for detecting nuleosomes containing histone modifications and variants
GB1518674.5 2015-10-21
PCT/GB2016/053288 WO2017068359A1 (en) 2015-10-21 2016-10-21 Method for detecting nucleosomes containing histone modifications and variants

Publications (1)

Publication Number Publication Date
CN108496083A true CN108496083A (zh) 2018-09-04

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680074822.1A Pending CN108496083A (zh) 2015-10-21 2016-10-21 用于检测包含组蛋白修饰和变体的核小体的方法

Country Status (6)

Country Link
US (1) US10768182B2 (enExample)
EP (1) EP3365681B1 (enExample)
JP (1) JP6925328B2 (enExample)
CN (1) CN108496083A (enExample)
GB (1) GB201518674D0 (enExample)
WO (1) WO2017068359A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113480646A (zh) * 2021-07-01 2021-10-08 中国科学院生物物理研究所 特异性结合h1.4移码突变蛋白的单克隆抗体制备及其应用
CN114829939A (zh) * 2019-12-02 2022-07-29 比利时意志有限责任公司 无细胞核小体作为生物标志物的用途
CN117904196A (zh) * 2024-01-05 2024-04-19 安徽大学 一种非小细胞肺癌组蛋白h1.4赖氨酸乙酰化点突变细胞模型的构建及其应用

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107250799B (zh) 2015-02-10 2019-09-10 B.R.A.H.M.S 有限公司 作为生物标志物的游离组蛋白
GB202108185D0 (en) 2021-06-08 2021-07-21 Belgian Volition Sprl Standardisation of nucleosome assays using biologically derived calibrants
GB202117799D0 (en) 2021-12-09 2022-01-26 Belgian Volition Srl Method for the detection of blood cancer
WO2023170298A1 (en) 2022-03-11 2023-09-14 Belgian Volition Srl Differential diagnosis method
GB202205212D0 (en) 2022-04-08 2022-05-25 Belgian Volition Sprl Method for transplant organ health assessment
WO2024133225A1 (en) 2022-12-19 2024-06-27 Belgian Volition Srl Calibration
WO2024213795A1 (en) 2023-04-14 2024-10-17 Belgian Volition Srl Diagnosis method using cell free nucleosome levels
WO2024223947A1 (en) 2023-04-28 2024-10-31 Belgian Volition Srl Use of cell free nucleosomes as biomarkers of carcinomas
GB202307768D0 (en) 2023-05-24 2023-07-05 Belgian Volition Srl Use of cell free nucleosomes as biomarkers
GB202310137D0 (en) 2023-07-03 2023-08-16 Belgian Volition Srl Method for the detection of cancer
WO2025061853A1 (en) 2023-09-19 2025-03-27 Belgian Volition Srl Lateral flow test device
WO2025094148A1 (en) * 2023-11-03 2025-05-08 Onchilles Pharma, Inc. Histone h1 levels as biomarkers for cancer therapy
WO2025181348A1 (en) 2024-03-01 2025-09-04 Belgian Volition Srl Method for determining the origin of circulating dna
WO2025253002A1 (en) 2024-06-07 2025-12-11 Belgian Volition Srl Sample collection for liquid biopsy

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011150974A1 (en) * 2010-06-03 2011-12-08 Porto Conte Ricerche S.R.L. Biomarkers for lung neuroendocrine tumors
US20130230858A1 (en) * 2012-03-02 2013-09-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN104053993A (zh) * 2011-09-01 2014-09-17 新加坡意志私人有限公司 用于检测含组蛋白变体的核小体的方法
CN104067124A (zh) * 2011-09-01 2014-09-24 新加坡意志私人有限公司 用于检测含核苷酸的核小体的方法
CN104067125A (zh) * 2011-12-07 2014-09-24 新加坡意志私人有限公司 用于检测核小体加合物的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0319376D0 (en) * 2003-08-18 2003-09-17 Chroma Therapeutics Ltd Histone modification detection
GB201303575D0 (en) 2013-02-28 2013-04-10 Singapore Volition Pte Ltd Method for detecting histone modifications in nucleosomes

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011150974A1 (en) * 2010-06-03 2011-12-08 Porto Conte Ricerche S.R.L. Biomarkers for lung neuroendocrine tumors
CN104053993A (zh) * 2011-09-01 2014-09-17 新加坡意志私人有限公司 用于检测含组蛋白变体的核小体的方法
CN104067124A (zh) * 2011-09-01 2014-09-24 新加坡意志私人有限公司 用于检测含核苷酸的核小体的方法
CN104067125A (zh) * 2011-12-07 2014-09-24 新加坡意志私人有限公司 用于检测核小体加合物的方法
US20130230858A1 (en) * 2012-03-02 2013-09-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
C GABLER等: "Extranuclear detection of histones and nucleosomes in activated human lymphoblasts as an early event in apoptosis", 《ANN RHEUM DIS》 *
DELIGEZER, UGUR 等: "Sequence-specific histone methylation is detectable on circulating nucleosomes in plasma", 《CLINICAL CHEMISTRY》 *
SAKAMOTO, JUN 等: "Immunoprecipitation of nucleosomal DNA is a novel procedure to improve the sensitivity of serum screening for the p16 hypermethylation associated with colon cancer", 《CANCER EPIDEMIOLOGY》 *
SCAFFIDI, PAOLA: "Histone H1 alterations in cancer", 《BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114829939A (zh) * 2019-12-02 2022-07-29 比利时意志有限责任公司 无细胞核小体作为生物标志物的用途
CN114829939B (zh) * 2019-12-02 2025-10-21 比利时意志有限责任公司 无细胞核小体作为生物标志物的用途
CN113480646A (zh) * 2021-07-01 2021-10-08 中国科学院生物物理研究所 特异性结合h1.4移码突变蛋白的单克隆抗体制备及其应用
CN113480646B (zh) * 2021-07-01 2022-03-15 中国科学院生物物理研究所 特异性结合h1.4移码突变蛋白的单克隆抗体制备及其应用
CN117904196A (zh) * 2024-01-05 2024-04-19 安徽大学 一种非小细胞肺癌组蛋白h1.4赖氨酸乙酰化点突变细胞模型的构建及其应用

Also Published As

Publication number Publication date
WO2017068359A1 (en) 2017-04-27
EP3365681B1 (en) 2023-06-07
EP3365681A1 (en) 2018-08-29
JP2018536849A (ja) 2018-12-13
JP6925328B2 (ja) 2021-08-25
US10768182B2 (en) 2020-09-08
US20190064183A1 (en) 2019-02-28
GB201518674D0 (en) 2015-12-02

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Application publication date: 20180904

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